RLG00000036355

Belongs to the peptidase M16 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000183
Physical Location & Seq
Reverse (-)
79625508 .. 79643729
18222 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000036355

Sequence Viewer

Length: 3603 bp
ATGGGTGTGATGGGTTGCTGCACTTTCTCGTCGGACGACATTGTAATAAAGTCTCCTAATGATAAACGACTCTCCAGAGTGATTAAGCTTGAGAATGGCCTTACTGCATTGCTCATTCACGATCCTCAGGTCTACCCACAAGGACCACCCCACCACTCCTCCGACCTTGAACAACAACAAGATAGTGAAGCTGTAGAAGAAGAAGAAGAGGAAGAGGAAGAAGAAGAAGATAGTGAAGCTGTAGAAGAAGAAGAAGAAGAGGAAGAGGAAGAGGAAGAGGAAGAGGAAGGTGAAGTTAAAAGGAAGGAAGAGGGAGGTGCTTCTGAGACTAAGAAGGCAGCAGCAGCAATGTGTGTCAGAATAGGCAGTTTCTCTGACCCTCTCGAGGCTCAGGGGCTTGCACATTTTCTAGAACACATGCTGTTCATGGGGAGTACAAAGTTCCCAGATGAAAATGAGTATGCTAGTTACTTGTCCAAGCATGGAGGGTCGTCAAATGCATACACAGAAGCAGAACATACTTGCTACTACTTTAATGTGAAACGAGAGTTTCTTAAGGGTGCCTTGAAAAGATTTTCTCAGTTCTTTGTTTCACCTCTAATGAAAAGTGAAGCCATGGAGAGAGAGGTGCAGGCTATAGACTCAGAGTTTAAAAAGGTTCTGCAAAACGATTTCTGCCGCCTTCGACAACTTCAGGGCCATACATCCTCACCTGGTCACCCATTTAATAAATTCTCTTGGGGAAATAAGAAGAGCTTGGATGATGCAAAGGAAAAAGGGATCAACTTGCAGGAACGAATACTGAAATTGTACAGAGACTATTACCACGGTGGATTAATGAAGCTAGTTGTCATTGGTGGAGAATCTCTTGATGTACTTGAGCACTGGGTTTTGGAATTGTTTGGAGATGTCAAAAAAGGTCCCCAAGTAAATCTGGAGTTCAAGGCAGAAGGTCCTATTTGGAAAGCTGGAAAACTTTACAGGCTAGAGGCTGTTGACAATGTTCATATACTCCACCTAGCATGGACACTTCCATGTCTTCAAGAACACTATTTGAGGAAACCAGAAGATTACTTGAGTCATCTGCTTGGGCATGAGGGCAGGGGAAGTTTGCATTTCTACCTGAAAGCTAGAGGGTGGGTAACATCTTTAGATACTTGTTCAAGCGGGATGGACTGCTCTTCTGTGGCTTATATCTTTTGCATGGTCATATACCTCACTGACTCTGGATTGGAGAAGATTTTTGAAATAATTGGGTTCGTGTATCAATACATTGAGTTTTTACGTCAAATGTCGCCACAAGAATGGATATTTCGGGAACTTCAGGATATTGGGAACATGGACTTTAGATTTGCAGAGGAGCAGGATCAGGATGATTATGCTTCACAACTTGCAGGAAATAAGAAGAGCCTGGATGATGCAACGAAAAAGGGGATCAACTTGCAGGAACAAATACTGAAATTGTACAGAAACTATTACCGCGGTGGATCAATGAAGCTAGTTGTCATTGGTGGAGAGTCGCTCGATGTACTGGAGAAGTGGATTTTGGAATTGTATAGAGATGTCAAAACCGGACCCCAAGTAAATCTGGAGTTCAAGGTAGACGGTCCTATTTGGAAAGCTGGAAAAGTTTACAGGCTAGAGGCTGTTCAAGATGTTCATATACTGCAATTGTCATGGAAACTTCCATGCCTTCTTCAACACTATTTGAAAGGCCCAGAATTTTATTTACATCATCTCCTTGTGCATGAGGGCAGGGGAAGTTTGCATTTCTACCTCAAAGCTAGAGGGTGGGCAACGTATCTAGGTGCTTCGGTTAGCCGCTATCCTGTGGCTGATGTCTTTTGCACGATCATATACCTCACTGACTCTGGACTGGAGAAGATTTTTGAAATAATTGGGTTGGTGTATCAATACATTAAGTTACTACGTCAGGAGACGCCAGAATGGATATTTCGGGAACTCCAGGATATAGGGAACATGTGCTTTAGATTTGCAGAGGAGGAGCCTTTCCAGGATGATTATGTTTCAAACCTTGCAGAATTTCAGTGTGAGCCTTGGTTCGGGTCACATTATACTGAGGAAGATATATCTCCAGCTTTGATGGAATTGTGGAAGGATCCTCCAGAAACTGATGTATCGTTGCATCTGCGGGCAAAAAATGAGTTCATTCCCAGTGACTTTTCCATCCGTTCTGATGGGATGTGTCTTGATATTTCAAATATATCTTCTCCGGCATGTATGTTTGATGAACCATTGATGAAGATCTGGTACAAGCTTGATAGTACTTTTAAACTTCCACGGGCAAATGCGTACTTCTTCATCAGAATGAAAGGTGGATATGATAATGTAAAAAGTAGTGTTTTGACAGACTTATACATTGACCTTCTCAAAGATGAGCTCAGTGAGATCATCTATCAGGCTTATGTTGCCTGCTTGGGGACTTCTATTTCTGTGTTTACCGACAAACTCCAGCTGCAGGTGTACGGTTTCAATGATAAGCTTCCAGCTCTGTTGTCAAAAATTGTGAAAACAGTAAAGAGCTTCTTGCCAACTGATGATCGTTTTGAGGTTATTAAAGAAGACATGGTGCGAGTGTATAGGAATGCCAATATGAATCCTTGGTGTCACTCAACATACTTGAGAGAGCAAGTTTTGTTGCAGAGTTTCTACAACGTAGATGAGCTGTTTCATGTTTTGAATGGGTTGTCTGTTTCTGATTTGAAGTCATTTATTCCTGAGCTTTTCTCCCAGATCTACATTGAGGGCCTTTTCCATGGTAATTTGTCAGAAGAAGAAGCAATTAGCCTTTCAAATTTATTCAAGACAATCTTTTCCGTAAAACCACTTCCTGTTGAATTGATGTATAGAAATCATTGTATTTGTCTTCCTCCTAATGCTAACCTCATTAGAGATGCTACTGTGAAGAACAAGTCAGAAACAAACTCTGTGACAGAGCTGTATTTTCAAATTGAGTGGGCCGTAGGGAGCGAGTCCGTGAGATGGAAAGTTTTGATAGATCTTTTCGACGCAGTTGTGAAGGAACCACTTTTTAATCAGCTAAGGACAAAGGAGCAGCTGGGGTATGGAGTTCAGTGTGGCCGGGATTTAAAATGGGGTGTTTTTGGCATTCTTTTCTCTGTTCAGTCATCTGAGTACAACCCCATCCACCTACAAGGAAGACTAGACAACTTCATAAATGGTCTGGAAGATTTGTTGGAAGGACTGGATGATGATTCCTTTGAGAATTATAAAGCTGGACTAATGGAAAATCTTTTGAAGAAAGATACATCCCTCACTTGTGAAACCAATCGATTGTGGAATGAGATTTATATTAAAAGGTATATGTTTGACTACTCAAAAAAGGCAGCAGAAGAACTCAGAAGCATTCAGAAGGAGGATGTTATCATTTTTTACCAGACATATTTGCAACAATCATCTCCCAAGTGTCGAAGACTTGCAACTCGTGTTTGGGGTTGCAACACCGACTTCAAAGAGGCAGAAGTAGCACGACTGGAGTCTGTGCAAATCATTGAAGACGTTGCAGCCTTTAAGATGTCATCGAAGTTCTATGAACCTAAAATAAATACTCTGGATACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0003674 GO:0003824 GO:0004175 GO:0004222 GO:0005102 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005777 GO:0005782 GO:0005829 GO:0006508 GO:0006518 GO:0006605 GO:0006625 GO:0006807 GO:0006810 GO:0006886 GO:0006996 GO:0007031 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007169 GO:0007275 GO:0007568 GO:0008104 GO:0008144 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0008270 GO:0008286 GO:0008340 GO:0009056 GO:0009057 GO:0009719 GO:0009725 GO:0009893 GO:0009894 GO:0009896 GO:0009986 GO:0009987 GO:0010033 GO:0010243 GO:0010259 GO:0010604 GO:0010815 GO:0010992 GO:0015031 GO:0015833 GO:0016043 GO:0016787 GO:0017046 GO:0017076 GO:0017144 GO:0019222 GO:0019538 GO:0019725 GO:0022607 GO:0023052 GO:0030163 GO:0030554 GO:0031334 GO:0031907 GO:0031974 GO:0032459 GO:0032461 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0032868 GO:0032869 GO:0032870 GO:0033036 GO:0033218 GO:0033365 GO:0034613 GO:0034641 GO:0035639 GO:0036094 GO:0042176 GO:0042221 GO:0042277 GO:0042562 GO:0042579 GO:0042592 GO:0042737 GO:0042802 GO:0042803 GO:0042886 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043171 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043254 GO:0043434 GO:0043559 GO:0043574 GO:0043603 GO:0043933 GO:0044085 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044421 GO:0044422 GO:0044424 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0045732 GO:0046872 GO:0046907 GO:0046914 GO:0046983 GO:0048518 GO:0048522 GO:0048856 GO:0050435 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051259 GO:0051260 GO:0051603 GO:0051641 GO:0051649 GO:0051716 GO:0060255 GO:0065003 GO:0065007 GO:0065008 GO:0070011 GO:0070013 GO:0070727 GO:0070887 GO:0071310 GO:0071375 GO:0071417 GO:0071495 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072662 GO:0072663 GO:0080090 GO:0097159 GO:0097242 GO:0097367 GO:0140030 GO:0140035 GO:0140036 GO:0140096 GO:1901142 GO:1901143 GO:1901265 GO:1901363 GO:1901564 GO:1901565 GO:1901575 GO:1901652 GO:1901653 GO:1901698 GO:1901699 GO:1901700 GO:1901701
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_M16 Peptidase_M16_C Peptidase_M16_M

Protein Analysis

1201

Amino Acids

138.55

Weight (kDa)

5.0

Isoelectric Point (pI)

48.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M16 PF00675 109 - 237 5.3e-34 Insulinase (Peptidase family M16)
Peptidase_M16_C PF05193 265 - 442 4.8e-18 Peptidase M16 inactive domain
Peptidase_M16_C PF05193 483 - 656 8.1e-16 Peptidase M16 inactive domain
Peptidase_M16_M PF16187 683 - 897 1.1e-59 Middle or third domain of peptidase_M16
Peptidase_M16_C PF05193 903 - 1088 7.3e-18 Peptidase M16 inactive domain
PqqF-like_C_4 PF22456 1009 - 1107 5.2e-22 PQQ synthase PqqF-like, C-terminal lobe domain 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000183)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G01440
fragaria_vesca FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40361 FvH4_3g40370 FvH4_3g40410 FvH4_3g40410 FvH4_3g40411 FvH4_3g40412 FvH4_3g40413 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310 FvH4_5g00310
rosa_chinensis RchiOBHm_Chr1g0321501 RchiOBHm_Chr1g0334011 RchiOBHm_Chr1g0334041 RchiOBHm_Chr1g0335131 RchiOBHm_Chr2g0145491 RchiOBHm_Chr3g0492731 RchiOBHm_Chr5g0072791 RchiOBHm_Chr5g0072891 RchiOBHm_Chr5g0072931 RchiOBHm_Chr5g0072951 RchiOBHm_Chr5g0073041 RchiOBHm_Chr5g0073051 RchiOBHm_Chr5g0073061 RchiOBHm_Chr5g0073071 RchiOBHm_Chr5g0074151 RchiOBHm_Chr5g0074161 RchiOBHm_Chr7g0202261
rosa_laevigata RLG00000014828 RLG00000036342 RLG00000036343 RLG00000036346 RLG00000036350 RLG00000036353 RLG00000036355 RLG00000036356
rosa_multiflora Rmu_co8227831.1_g000001 Rmu_co8517625.1_g000001 Rmu_sc0001113.1_g000013 Rmu_sc0001394.1_g000005 Rmu_sc0002548.1_g000005 Rmu_sc0003160.1_g000003 Rmu_sc0003160.1_g000023 Rmu_sc0005069.1_g000004 Rmu_sc0005069.1_g000026 Rmu_sc0005592.1_g000031 Rmu_sc0005592.1_g000032 Rmu_sc0005592.1_g000033 Rmu_sc0007034.1_g000002 Rmu_sc0008926.1_g000001 Rmu_sc0008926.1_g000005 Rmu_sc0010523.1_g000004 Rmu_sc0010523.1_g000005 Rmu_sc0010900.1_g000006 Rmu_sc0010900.1_g000008 Rmu_sc0018267.1_g000001 Rmu_sc0040908.1_g000001
rosa_roxburghii Rroxscaffold_1G00008160 Rroxscaffold_1G00008170 Rroxscaffold_1G00008240 Rroxscaffold_1G00008250 Rroxscaffold_1G00008280 Rroxscaffold_1G00008290 Rroxscaffold_1G00008310 Rroxscaffold_1G00008340 Rroxscaffold_1G00008360 Rroxscaffold_2G00147720 Rroxscaffold_3G00254700 Rroxscaffold_5G00358330
rosa_rugosa Rorug01G0030700 Rorug03G0256700 Rorug03G0282500 Rorug04G0070500 Rorug05G0104700 Rorug05G0241200 Rorug05G0414700 Rorug05G0418900 Rorug05G0420400 Rorug05G0420500 Rorug05G0420500 Rorug05G0420700 Rorug05G0420800 Rorug05G0420900 Rorug06G0037800 Rorug07G0069500 Rorug07G0069600 Rorug07G0069600
rosa_samantha Rh2DG665200 Rh3AG306000 Rh3DG242400 Rh5AG458000 Rh5AG477100 Rh5AG477200 Rh5AG477400 Rh5AG477500 Rh5AG477600 Rh5AG487100 Rh5AG501000 Rh5BG497200 Rh5BG497400 Rh5BG497600 Rh5BG497700 Rh5BG498100 Rh5BG498200 Rh5BG498300 Rh5BG498400 Rh5CG521500 Rh5DG501800 Rh5DG509300 Rh5DG510000 Rh5DG510100 Rh5DG510400 Rh5DG520000 Rh6BG100200 Rh6BG523800 Rh6CG196600 Rh7AG255600 Rh7AG267200 Rh7DG203300
rosa_wichuraiana Rw1G031050 Rw5G044330 Rw5G044340 Rw5G044360 Rw5G044370 Rw5G046550 Rw7G017220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3252
AarI CACCTGC 1 cut(s) 2470
Acc36I ACCTGC 1 cut(s) 2470
AccB1I GGYRCC 1 cut(s) 560
AccI GTMKAC 2 cut(s) 132, 1602
AccII CGCG 1 cut(s) 1482
AciI CCGC 6 cut(s) 679, 1167, 1480, 1482, 1822, 2152
AclWI GGATC 7 cut(s) 116, 788, 1374, 1442, 1495, 2114, 2127
AcoI YGGCCR 1 cut(s) 3100
AcsI RAATTY 4 cut(s) 731, 1721, 2042, 2815
AcuI CTGAAG 2 cut(s) 677, 1307
AcyI GRCGYC 1 cut(s) 1940
AfiI CCNNNNNNNGG 5 cut(s) 385, 2063, 2439, 2479, 3003
AflII CTTAAG 1 cut(s) 554
AflIII ACRYGT 1 cut(s) 1980
AjnI CCWGG 4 cut(s) 712, 1410, 1965, 2013
AjuI GAANNNNNNNTTGG 2 cut(s) 1529, 1561
Alw21I GWGCWC 2 cut(s) 885, 2403
Alw26I GTCTC 4 cut(s) 57, 320, 810, 1931
AlwI GGATC 7 cut(s) 116, 788, 1374, 1442, 1495, 2114, 2127
AlwNI CAGNNNCTG 1 cut(s) 2132
Ama87I CYCGRG 1 cut(s) 383
AoxI GGCC 6 cut(s) 97, 697, 1714, 2767, 2979, 3100
ApeKI GCWGC 8 cut(s) 18, 338, 341, 344, 2476, 3076, 3368, 3545
ApoI RAATTY 4 cut(s) 731, 1721, 2042, 2815
ArsI GACNNNNNNTTYG 2 cut(s) 1334, 1366
AseI ATTAAT 1 cut(s) 836
AspS9I GGNCC 9 cut(s) 143, 697, 920, 953, 1574, 1607, 1715, 2767, 2979
AsuC2I CCSGG 1 cut(s) 3104
AsuHPI GGTGA 4 cut(s) 302, 585, 702, 710
AvaI CYCGRG 1 cut(s) 383
AvaII GGWCC 5 cut(s) 143, 920, 953, 1574, 1607
AxyI CCTNAGG 1 cut(s) 126
BaeI ACNNNNGTAYC 2 cut(s) 2121, 2154
BamHI GGATCC 1 cut(s) 2119
BanI GGYRCC 1 cut(s) 560
BanII GRGCYC 1 cut(s) 2403
BarI GAAGNNNNNNTAC 4 cut(s) 2255, 2287, 2832, 2864
BauI CACGAG 1 cut(s) 3465
BbsI GAAGAC 6 cut(s) 1031, 2589, 2879, 3187, 3460, 3543
Bbv12I GWGCWC 2 cut(s) 885, 2403
BbvI GCAGC 8 cut(s) 5, 350, 353, 356, 2463, 3088, 3380, 3557
BccI CCATC 7 cut(s) 4, 1165, 2098, 2192, 2195, 2997, 3173
BceAI ACGGC 1 cut(s) 2966
BciT130I CCWGG 4 cut(s) 714, 1412, 1967, 2015
BciVI GTATCC 1 cut(s) 3589
BcnI CCSGG 1 cut(s) 3104
BcoDI GTCTC 4 cut(s) 57, 320, 810, 1931
BfmI CTRYAG 4 cut(s) 192, 240, 636, 2477
BfrI CTTAAG 1 cut(s) 554
BfuAI ACCTGC 1 cut(s) 2470
BfuI GTATCC 1 cut(s) 3589
BglII AGATCT 3 cut(s) 2265, 2754, 3019
BmcAI AGTACT 1 cut(s) 2287
Bme1390I CCNGG 5 cut(s) 714, 1412, 1967, 2015, 3104
Bme18I GGWCC 5 cut(s) 143, 920, 953, 1574, 1607
BmeT110I CYCGRG 1 cut(s) 383
BmgT120I GGNCC 9 cut(s) 143, 697, 920, 953, 1574, 1607, 1715, 2767, 2979
BmiI GGNNCC 6 cut(s) 562, 922, 1576, 2007, 2121, 3045
BmrFI CCNGG 5 cut(s) 714, 1412, 1967, 2015, 3104
BmrI ACTGGG 2 cut(s) 895, 2169
BmsI GCATC 4 cut(s) 754, 1408, 2155, 2905
BmuI ACTGGG 2 cut(s) 895, 2169
BoxI GACNNNNGTC 1 cut(s) 3517
BpiI GAAGAC 6 cut(s) 1031, 2589, 2879, 3187, 3460, 3543
BplI GAGNNNNNCTC 4 cut(s) 1506, 1538, 2732, 2764
Bpu10I CCTNAGC 3 cut(s) 390, 2739, 3062
BpuEI CTTGAG 4 cut(s) 110, 899, 1096, 2662
BpuMI CCSGG 1 cut(s) 3104
Bsa29I ATCGAT 1 cut(s) 3313
BsaBI GATNNNNATC 1 cut(s) 2264
BsaHI GRCGYC 1 cut(s) 1940
BsaJI CCNNGG 7 cut(s) 615, 826, 1480, 2057, 2300, 2621, 2776
BsaWI WCCGGW 1 cut(s) 1571
BsaXI ACNNNNNCTCC 4 cut(s) 143, 173, 1722, 1752
Bsc4I CCNNNNNNNGG 5 cut(s) 385, 2063, 2439, 2479, 3003
Bse1I ACTGG 6 cut(s) 890, 1536, 1881, 2175, 3231, 3519
Bse21I CCTNAGG 1 cut(s) 126
Bse3DI GCAATG 2 cut(s) 107, 354
Bse8I GATNNNNATC 1 cut(s) 2264
BseBI CCWGG 4 cut(s) 714, 1412, 1967, 2015
BseCI ATCGAT 1 cut(s) 3313
BseDI CCNNGG 7 cut(s) 615, 826, 1480, 2057, 2300, 2621, 2776
BseJI GATNNNNATC 1 cut(s) 2264
BseLI CCNNNNNNNGG 5 cut(s) 385, 2063, 2439, 2479, 3003
BseMI GCAATG 2 cut(s) 107, 354
BseNI ACTGG 6 cut(s) 890, 1536, 1881, 2175, 3231, 3519
BseRI GAGGAG 4 cut(s) 148, 1373, 2015, 2018
BseXI GCAGC 8 cut(s) 5, 350, 353, 356, 2463, 3088, 3380, 3557
BseYI CCCAGC 1 cut(s) 3079
BsgI GTGCAG 2 cut(s) 4, 650
Bsh1236I CGCG 1 cut(s) 1482
BshFI GGCC 6 cut(s) 99, 699, 1716, 2769, 2981, 3102
BshNI GGYRCC 1 cut(s) 560
BshVI ATCGAT 1 cut(s) 3313
BsiHKAI GWGCWC 2 cut(s) 885, 2403
BsiHKCI CYCGRG 1 cut(s) 383
BsiSI CCGG 3 cut(s) 1572, 2234, 3103
BslFI GGGAC 2 cut(s) 906, 2455
BslI CCNNNNNNNGG 5 cut(s) 385, 2063, 2439, 2479, 3003
BsmAI GTCTC 4 cut(s) 57, 320, 810, 1931
BsmBI CGTCTC 1 cut(s) 1931
BsmFI GGGAC 2 cut(s) 906, 2455
BsmI GAATGC 3 cut(s) 2611, 3129, 3387
BsnI GGCC 6 cut(s) 99, 699, 1716, 2769, 2981, 3102
BsoBI CYCGRG 1 cut(s) 383
Bsp1286I GDGCHC 2 cut(s) 885, 2403
Bsp1407I TGTACA 2 cut(s) 810, 1464
Bsp19I CCATGG 2 cut(s) 615, 2776
BspACI CCGC 6 cut(s) 679, 1167, 1480, 1482, 1822, 2152
BspANI GGCC 6 cut(s) 99, 699, 1716, 2769, 2981, 3102
BspDI ATCGAT 1 cut(s) 3313
BspFNI CGCG 1 cut(s) 1482
BspLI GGNNCC 6 cut(s) 562, 922, 1576, 2007, 2121, 3045
BspMAI CTGCAG 1 cut(s) 2481
BspMI ACCTGC 1 cut(s) 2470
BspPI GGATC 7 cut(s) 116, 788, 1374, 1442, 1495, 2114, 2127
BspQI GCTCTTC 3 cut(s) 746, 1186, 1400
BspT107I GGYRCC 1 cut(s) 560
BspTI CTTAAG 1 cut(s) 554
BsrDI GCAATG 2 cut(s) 107, 354
BsrGI TGTACA 2 cut(s) 810, 1464
BsrI ACTGG 6 cut(s) 890, 1536, 1881, 2175, 3231, 3519
BssECI CCNNGG 7 cut(s) 615, 826, 1480, 2057, 2300, 2621, 2776
BssNI GRCGYC 1 cut(s) 1940
BssSI CACGAG 1 cut(s) 3465
BssT1I CCWWGG 4 cut(s) 615, 2057, 2621, 2776
Bst2BI CACGAG 1 cut(s) 3465
Bst2UI CCWGG 4 cut(s) 714, 1412, 1967, 2015
Bst4CI ACNGT 5 cut(s) 830, 1607, 2489, 2536, 2923
BstACI GRCGYC 1 cut(s) 1940
BstAFI CTTAAG 1 cut(s) 554
BstAUI TGTACA 2 cut(s) 810, 1464
BstC8I GCNNGC 4 cut(s) 399, 633, 2154, 2434
BstDSI CCRYGG 5 cut(s) 615, 826, 1480, 2300, 2776
BstEII GGTNACC 1 cut(s) 716
BstFNI CGCG 1 cut(s) 1482
BstMAI GTCTC 4 cut(s) 57, 320, 810, 1931
BstMWI GCNNNNNNNGC 3 cut(s) 344, 2429, 3506
BstNI CCWGG 4 cut(s) 714, 1412, 1967, 2015
BstNSI RCATGY 3 cut(s) 421, 1984, 2241
BstPAI GACNNNNGTC 1 cut(s) 3517
BstPI GGTNACC 1 cut(s) 716
BstSCI CCNGG 5 cut(s) 712, 1410, 1965, 2013, 3102
BstSFI CTRYAG 4 cut(s) 192, 240, 636, 2477
BstUI CGCG 1 cut(s) 1482
BstV1I GCAGC 8 cut(s) 5, 350, 353, 356, 2463, 3088, 3380, 3557
BstV2I GAAGAC 6 cut(s) 1031, 2589, 2879, 3187, 3460, 3543
BstX2I RGATCY 4 cut(s) 2119, 2265, 2754, 3019
BstXI CCANNNNNNTGG 1 cut(s) 1305
BstYI RGATCY 4 cut(s) 2119, 2265, 2754, 3019
Bsu15I ATCGAT 1 cut(s) 3313
Bsu36I CCTNAGG 1 cut(s) 126
BsuI GTATCC 1 cut(s) 3589
BsuRI GGCC 6 cut(s) 99, 699, 1716, 2769, 2981, 3102
BsuTUI ATCGAT 1 cut(s) 3313
BtgI CCRYGG 5 cut(s) 615, 826, 1480, 2300, 2776
BtsIMutI CAGTG 7 cut(s) 883, 1218, 1863, 2054, 2182, 2410, 3101
BveI ACCTGC 1 cut(s) 2470
Cac8I GCNNGC 4 cut(s) 399, 633, 2154, 2434
CaiI CAGNNNCTG 1 cut(s) 2132
Cfr13I GGNCC 9 cut(s) 143, 697, 920, 953, 1574, 1607, 1715, 2767, 2979
Cfr42I CCGCGG 1 cut(s) 1483
ClaI ATCGAT 1 cut(s) 3313
CseI GACGC 2 cut(s) 1948, 3038
CsiI ACCWGGT 1 cut(s) 712
CspCI CAANNNNNGTGG 2 cut(s) 2957, 2992
DraI TTTAAA 3 cut(s) 652, 2293, 3111
EaeI YGGCCR 1 cut(s) 3100
Ecl136II GAGCTC 1 cut(s) 2401
Eco130I CCWWGG 4 cut(s) 615, 2057, 2621, 2776
Eco24I GRGCYC 1 cut(s) 2403
Eco47I GGWCC 5 cut(s) 143, 920, 953, 1574, 1607
Eco53kI GAGCTC 1 cut(s) 2401
Eco57I CTGAAG 2 cut(s) 677, 1307
Eco81I CCTNAGG 1 cut(s) 126
Eco88I CYCGRG 1 cut(s) 383
Eco91I GGTNACC 1 cut(s) 716
EcoICRI GAGCTC 1 cut(s) 2401
EcoO109I RGGNCCY 3 cut(s) 920, 953, 2767
EcoO65I GGTNACC 1 cut(s) 716
EcoRII CCWGG 4 cut(s) 712, 1410, 1965, 2013
EcoT14I CCWWGG 4 cut(s) 615, 2057, 2621, 2776
EcoT22I ATGCAT 1 cut(s) 502
EcoT38I GRGCYC 1 cut(s) 2403
ErhI CCWWGG 4 cut(s) 615, 2057, 2621, 2776
Esp3I CGTCTC 1 cut(s) 1931
FalI AAGNNNNNCTT 8 cut(s) 548, 580, 740, 772, 2531, 2563, 2816, 2848
FaqI GGGAC 2 cut(s) 906, 2455
FauI CCCGC 2 cut(s) 1160, 2145
FblI GTMKAC 2 cut(s) 132, 1602
FriOI GRGCYC 1 cut(s) 2403
GsaI CCCAGC 1 cut(s) 3083
HaeIII GGCC 6 cut(s) 99, 699, 1716, 2769, 2981, 3102
HapII CCGG 3 cut(s) 1572, 2234, 3103
HgaI GACGC 2 cut(s) 1948, 3038
Hin1I GRCGYC 1 cut(s) 1940
HincII GTYRAC 1 cut(s) 997
HindII GTYRAC 1 cut(s) 997
HindIII AAGCTT 3 cut(s) 86, 2276, 2501
HpaII CCGG 3 cut(s) 1572, 2234, 3103
HphI GGTGA 4 cut(s) 302, 585, 702, 710
Hpy166II GTNNAC 6 cut(s) 133, 997, 1603, 1633, 2460, 2485
Hpy8I GTNNAC 6 cut(s) 133, 997, 1603, 1633, 2460, 2485
Hpy99I CGWCG 2 cut(s) 34, 3032
HpyCH4III ACNGT 5 cut(s) 830, 1607, 2489, 2536, 2923
HpyCH4IV ACGT 5 cut(s) 1285, 1799, 1930, 2676, 3540
HpyF10VI GCNNNNNNNGC 3 cut(s) 344, 2429, 3506
HpySE526I ACGT 5 cut(s) 1285, 1799, 1930, 2676, 3540
Hsp92I GRCGYC 1 cut(s) 1940
KspI CCGCGG 1 cut(s) 1483
LguI GCTCTTC 3 cut(s) 746, 1186, 1400
LmnI GCTCC 4 cut(s) 1360, 2005, 2988, 3073
Lsp1109I GCAGC 8 cut(s) 5, 350, 353, 356, 2463, 3088, 3380, 3557
LweI GCATC 4 cut(s) 754, 1408, 2155, 2905
MabI ACCWGGT 1 cut(s) 712
MaeII ACGT 5 cut(s) 1285, 1799, 1930, 2676, 3540
MaeIII GTNAC 8 cut(s) 467, 716, 1141, 1923, 2067, 2177, 2627, 2950
MfeI CAATTG 1 cut(s) 1670
MflI RGATCY 4 cut(s) 2119, 2265, 2754, 3019
MhlI GDGCHC 2 cut(s) 885, 2403
MlyI GAGTC 8 cut(s) 63, 635, 1087, 1217, 1526, 1862, 3002, 3527
MmeI TCCRAC 3 cut(s) 12, 186, 3198
Mph1103I ATGCAT 1 cut(s) 502
MslI CAYNNNNRTG 3 cut(s) 1033, 1303, 2327
MspA1I CMGCKG 3 cut(s) 1482, 2476, 3079
MspCI CTTAAG 1 cut(s) 554
MspI CCGG 3 cut(s) 1572, 2234, 3103
MspR9I CCNGG 5 cut(s) 714, 1412, 1967, 2015, 3104
MunI CAATTG 1 cut(s) 1670
Mva1269I GAATGC 3 cut(s) 2611, 3129, 3387
MvaI CCWGG 4 cut(s) 714, 1412, 1967, 2015
MvnI CGCG 1 cut(s) 1482
MwoI GCNNNNNNNGC 3 cut(s) 344, 2429, 3506
NciI CCSGG 1 cut(s) 3104
NcoI CCATGG 2 cut(s) 615, 2776
NlaIV GGNNCC 6 cut(s) 562, 922, 1576, 2007, 2121, 3045
NmuCI GTSAC 5 cut(s) 716, 2067, 2177, 2627, 2950
NsiI ATGCAT 1 cut(s) 502
NspI RCATGY 3 cut(s) 421, 1984, 2241
PaeR7I CTCGAG 1 cut(s) 383
PaqCI CACCTGC 1 cut(s) 2470
PciI ACATGT 1 cut(s) 1980
PciSI GCTCTTC 3 cut(s) 746, 1186, 1400
PctI GAATGC 3 cut(s) 2611, 3129, 3387
PfeI GAWTC 3 cut(s) 863, 2617, 3236
PfoI TCCNGGA 2 cut(s) 1965, 2013
PleI GAGTC 8 cut(s) 63, 635, 1086, 1217, 1525, 1862, 3001, 3526
PpsI GAGTC 8 cut(s) 63, 635, 1086, 1217, 1525, 1862, 3001, 3526
PpuMI RGGWCCY 2 cut(s) 920, 953
PscI ACATGT 1 cut(s) 1980
PshAI GACNNNNGTC 1 cut(s) 3517
PshBI ATTAAT 1 cut(s) 836
PsiI TTATAA 1 cut(s) 3252
Psp124BI GAGCTC 1 cut(s) 2403
Psp5II RGGWCCY 2 cut(s) 920, 953
Psp6I CCWGG 4 cut(s) 712, 1410, 1965, 2013
PspEI GGTNACC 1 cut(s) 716
PspFI CCCAGC 1 cut(s) 3079
PspGI CCWGG 4 cut(s) 712, 1410, 1965, 2013
PspN4I GGNNCC 6 cut(s) 562, 922, 1576, 2007, 2121, 3045
PspPI GGNCC 9 cut(s) 143, 697, 920, 953, 1574, 1607, 1715, 2767, 2979
PspPPI RGGWCCY 2 cut(s) 920, 953
PstI CTGCAG 1 cut(s) 2481
PstNI CAGNNNCTG 1 cut(s) 2132
PsuI RGATCY 4 cut(s) 2119, 2265, 2754, 3019
PvuII CAGCTG 2 cut(s) 2476, 3079
RseI CAYNNNNRTG 3 cut(s) 1033, 1303, 2327
SacI GAGCTC 1 cut(s) 2403
SacII CCGCGG 1 cut(s) 1483
SapI GCTCTTC 3 cut(s) 746, 1186, 1400
Sau96I GGNCC 9 cut(s) 143, 697, 920, 953, 1574, 1607, 1715, 2767, 2979
ScaI AGTACT 1 cut(s) 2287
SchI GAGTC 8 cut(s) 63, 635, 1087, 1217, 1526, 1862, 3002, 3527
ScrFI CCNGG 5 cut(s) 714, 1412, 1967, 2015, 3104
SduI GDGCHC 2 cut(s) 885, 2403
SexAI ACCWGGT 1 cut(s) 712
SfaNI GCATC 4 cut(s) 754, 1408, 2155, 2905
SfcI CTRYAG 4 cut(s) 192, 240, 636, 2477
Sfr274I CTCGAG 1 cut(s) 383
Sfr303I CCGCGG 1 cut(s) 1483
SgrBI CCGCGG 1 cut(s) 1483
SinI GGWCC 5 cut(s) 143, 920, 953, 1574, 1607
SlaI CTCGAG 1 cut(s) 383
SmiMI CAYNNNNRTG 3 cut(s) 1033, 1303, 2327
SmlI CTYRAG 6 cut(s) 89, 383, 554, 878, 1075, 2641
SmoI CTYRAG 6 cut(s) 89, 383, 554, 878, 1075, 2641
SsiI CCGC 6 cut(s) 679, 1167, 1480, 1482, 1822, 2152
SstI GAGCTC 1 cut(s) 2403
StyD4I CCNGG 5 cut(s) 712, 1410, 1965, 2013, 3102
StyI CCWWGG 4 cut(s) 615, 2057, 2621, 2776
TaaI ACNGT 5 cut(s) 830, 1607, 2489, 2536, 2923
TaiI ACGT 5 cut(s) 1288, 1802, 1933, 2679, 3543
TaqI TCGA 7 cut(s) 384, 685, 1524, 3027, 3313, 3451, 3563
TatI WGTACW 7 cut(s) 434, 810, 874, 1464, 1528, 2285, 3156
TauI GCSGC 2 cut(s) 681, 1824
TfiI GAWTC 3 cut(s) 863, 2617, 3236
TscAI CASTG 7 cut(s) 890, 1225, 1870, 2054, 2182, 2410, 3101
TseFI GTSAC 5 cut(s) 716, 2067, 2177, 2627, 2950
TseI GCWGC 8 cut(s) 18, 338, 341, 344, 2476, 3076, 3368, 3545
Tsp45I GTSAC 5 cut(s) 716, 2067, 2177, 2627, 2950
TspGWI ACGGA 3 cut(s) 2180, 2827, 2986
TspRI CASTG 7 cut(s) 890, 1225, 1870, 2054, 2182, 2410, 3101
Vha464I CTTAAG 1 cut(s) 554
VpaK11BI GGWCC 5 cut(s) 143, 920, 953, 1574, 1607
VspI ATTAAT 1 cut(s) 836
XapI RAATTY 4 cut(s) 731, 1721, 2042, 2815
XbaI TCTAGA 1 cut(s) 409
XceI RCATGY 3 cut(s) 421, 1984, 2241
XhoI CTCGAG 1 cut(s) 383
XmiI GTMKAC 2 cut(s) 132, 1602
ZrmI AGTACT 1 cut(s) 2287
Zsp2I ATGCAT 1 cut(s) 502
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.