Prupe.6G041900_v2.0.a1

Belongs to the peptidase M16 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0000891
Physical Location & Seq
Forward (+)
3085883 .. 3094694
8812 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G041900.1

Sequence Viewer

Length: 3111 bp
ATGGGCCGTTGCACTTTCTCGTCAGACAATATCGTAATAACTCCTCCGAATGATATACGATCATACAGACTAATTGAGCTCGAGAATGGCCTCACTGCATTGCTCGTTCACGATCCTGAAATTTACCCAGAAGGGCCACCTGAACAAGCTGAAGAAGAATTAGAGGAAGATGAGGGCGGCAAGGATGGAGAGGCAGAGGAACATAGGGAAGGAGAGGTTGAAGAGAACGGTGTAGGAGATGAAGATGATGAACAACCGGAAGGTGGAGACGGTGAACTGAGAAGGAAGAGAAAGGGAAAGGGAGGTGCTTCCCAGACCAAGAGAAAGAGAAAGGGAGATGCTTCCCAAACCAAGAAGGCAGCTGCAGCAATGTCCATAGGAATAGGCAGCTTCTCTGACCCGATCGAGGCACAGGGACTTGCGCATTTTCTTGAACACATGCTTTTCATGGGGAGTACAGAGTTTCCAGATGAAAATGAGTATGATAGTTACTTGTCCAAGCATGGAGGGTCATCAAATGCATACACAGAAGCAGAGCATACCTGCTACCATTTTGAAGTGAAACAGGAGTTTCTTAAGGGTGCGTTGAGAAGATTTTCTCAGTTCTTTGTTTCCCCCCTAATGAAAATTGAAGCCATGGAGCGAGAGGTACAGGCTGTAGATTCAGAATTTAACCAGGTTTTGCAGAATGAGTCGTGCCGCCTTGAACAATTTCACTGCCATACAGCCGCACCTGGTCACCCATTTAATAAGTTCTTTTGGGGAAATAAGAAGAGCTTGGTTGATGCAATGGAAAATGGGATCAACTTGCGCGAACGAATACTAAAATTGTACAGAGATTATTACCATGGTGGACTGATGAAGCTAGTTGTCATTGGTGGAGAATCTCTTGATATACTTGAGGATTGGGTTGTGGAGTTGTTTACTAATGTCAAAAAAGGTCCCCAAGAAAAGCTGCAGTTCAAGGCAGAAGGCCCTATCTGGAAAGCTGGAAAACTTTACAGGCTAGAGCCTGTTAGAGATTTTCATATGCTCTACTTGACATGGACACTTCCATGTCTTCATCAAGAATATTTGAAGAAACCAGAAGATTATTTAGCTCATCTCCTTGGACACGAGGGCCGGGGAAGTTTGCATTTTTACCTCAAAGCTAGAGGGTGGGCAACATATTTGGCTGCTGGTGCTGGCGGTGGATGTATTTACCAAACTTCTGTGGCTTATGTCTTCAACATGACCATACACCTCACTGATTCTGGATTGGAAAAGATGTTTGACGTAATTGGCATTGTGTATCAATACATTAAGTTACTGCATCAAGTGTCTCCACAAGAATGGATATTTAGGGAACTCCAGGATATTGGAAATATGGAATTTAAATTCTCAGAGGAACCGGCCCAGGATTCTTATGCTTCAGGACTTGCAGGAAGTTTACTATTTTATCCAGCAAAATATATAATTTATGGTGGCTATGCATACGAGAGTTGGGATGAGGAATTGATAAAACATGTTCTTGGTTTCTTCACACCAGATAACATGAGGGTTGATTTGGTATCAAAGTCCTCAATTAAGTCAGAAGATTTCCAGTGCGAGCCTTGGTTTGGGTCAAAATATACTGAGGAGGATATATCTCCTTCTTTGATGGATTTGTGGAAGGATCCTCTAGAAATTGATGTTTCATTGCACCTCCCTTCTAAAAACGAGTTCATTCCTTTTGATTTTTCCATCCGTTCCGATAACTCGTGTCATGATCCTGCAAATATATCTTCCCCAAGATGTATAATTGATGAACCATTAATGAAGCTCTGGTACAAGCTTGATACCACTTTTAAGCTTCCGCGGGTAAATACATACTTCCGCATCAGTCTGAAGGGTGGATGTGCTAATCTGAGGAATTCTATTTTGATGGAACTATATGGTCGCCTTCTTAGAGATGAGCTGAATGAGATTATATATCAGGCACTGCTTGCCAATCTGGAAGCTTATGTAGGTCCGGTTGGTGAAAAACTGGAGATCAAGGTCAGTGGTTTCAACGATAAGCTTCCAGCTCTCTTATCAAAAATCTTGGCCACAGTCAAAAATTTTTTGCCAACCGATGATCGCTTGAAGGTTATTAAAGAAAATATGAAGCGAAGGATAAAGAACACGAATATGAATCCTCTGGTGCACACATCATACTTGAGACTGCAAGTTCTGTGGCAGACTTTCTATGATCCAGATGACAAGTTGCATGTTTTAGACGAATTGTCTATTTCCGATTTGAATTCATTTATTCCGGAGCTTTGGTCCCAGGTATTCATTGAGGGCCTTTGCCATGGCAATATGTTAGAAGAAGAAGCAATTAGCCTTTCAAATATATTCAAAATGAATTTTTCCGGACAACCACTTCCTACCGAATTGTGGCATAGAGAACGTGTTCTTTGTCTTCCTCCTGGTGCTAACCTGGTCAGAGATGCCAGTGTGAAGAACAAGTCGGAAACAAACTCTGTAACTGAGCTGCATTTTCAAATTAAGCAGGACGTGTTGACCACAATGAACGGAGTGATAGATCTTTTGATTGCAATTATATGGGAACCACTTTTCAATCAGCTAAGGACGAAGGAGCAGCTTGGATATATTGTTGAGTGTGGCCCACGGATAACATACCGTGTTTTCGGCTTTATTTTCTATGTTCAGTCGTCTGAGTACAGCCCAGTCTACTTACAAGAGAGAATTGACAACTTTATAGATGGTCTGGAAGAATTGTTGGAAGGGATTGATGATGATTTGTTTGAAAATTATAGAAGTGGGCTAATGGCTGGCCTATTGGAGAAAGATCTATCCCTCACTTGTGAAACCGATAGATATTGGTGTGAGATTGTTGGAAAAAGGTATATGTTTGACTTTGCTGCAGAGGAAGCAGAAGAGCTCAAAACCATTCACAAGGAGGATGTTATCAACTGGTACAGAACATATCTACAACAATCATCGCCCAAGTGTCGTAGACTTGCGACTCGGGTTTGGGGTTGCAACACTGACCCGAAAGAGGCTGAAGCGCGATCGGAGTCGATGAAAGTCATCGAAGACCCTGCAACCTTCAAGATGTCATCTACGTTCTACCCCAGCCTCTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0003674 GO:0003824 GO:0004175 GO:0004222 GO:0005102 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005777 GO:0005782 GO:0005829 GO:0006508 GO:0006518 GO:0006605 GO:0006625 GO:0006807 GO:0006810 GO:0006886 GO:0006996 GO:0007031 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007169 GO:0007275 GO:0007568 GO:0008104 GO:0008144 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0008270 GO:0008286 GO:0008340 GO:0009056 GO:0009057 GO:0009719 GO:0009725 GO:0009893 GO:0009894 GO:0009896 GO:0009986 GO:0009987 GO:0010033 GO:0010243 GO:0010259 GO:0010604 GO:0010815 GO:0010992 GO:0015031 GO:0015833 GO:0016043 GO:0016787 GO:0017046 GO:0017076 GO:0017144 GO:0019222 GO:0019538 GO:0019725 GO:0022607 GO:0023052 GO:0030163 GO:0030554 GO:0031334 GO:0031907 GO:0031974 GO:0032459 GO:0032461 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0032868 GO:0032869 GO:0032870 GO:0033036 GO:0033218 GO:0033365 GO:0034613 GO:0034641 GO:0035639 GO:0036094 GO:0042176 GO:0042221 GO:0042277 GO:0042562 GO:0042579 GO:0042592 GO:0042737 GO:0042802 GO:0042803 GO:0042886 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043171 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043254 GO:0043434 GO:0043559 GO:0043574 GO:0043603 GO:0043933 GO:0044085 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044421 GO:0044422 GO:0044424 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0045732 GO:0046872 GO:0046907 GO:0046914 GO:0046983 GO:0048518 GO:0048522 GO:0048856 GO:0050435 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051259 GO:0051260 GO:0051603 GO:0051641 GO:0051649 GO:0051716 GO:0060255 GO:0065003 GO:0065007 GO:0065008 GO:0070011 GO:0070013 GO:0070727 GO:0070887 GO:0071310 GO:0071375 GO:0071417 GO:0071495 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072662 GO:0072663 GO:0080090 GO:0097159 GO:0097242 GO:0097367 GO:0140030 GO:0140035 GO:0140036 GO:0140096 GO:1901142 GO:1901143 GO:1901265 GO:1901363 GO:1901564 GO:1901565 GO:1901575 GO:1901652 GO:1901653 GO:1901698 GO:1901699 GO:1901700 GO:1901701
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_M16 Peptidase_M16_C Peptidase_M16_M

Protein Analysis

1037

Amino Acids

118.85

Weight (kDa)

5.05

Isoelectric Point (pI)

46.62

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 423
Acc36I ACCTGC 1 cut(s) 551
AccI GTMKAC 2 cut(s) 2694, 2980
AccII CGCG 3 cut(s) 813, 1837, 3034
AccIII TCCGGA 2 cut(s) 2272, 2372
AciI CCGC 7 cut(s) 177, 700, 729, 1188, 1835, 1837, 1855
AclWI GGATC 6 cut(s) 107, 809, 1649, 1662, 1742, 2204
AcoI YGGCCR 1 cut(s) 2064
AcsI RAATTY 8 cut(s) 120, 668, 1370, 1376, 1891, 2077, 2260, 2365
AcuI CTGAAG 4 cut(s) 171, 1395, 1886, 3048
AfaI GTAC 6 cut(s) 457, 651, 833, 1808, 2684, 2942
AfiI CCNNNNNNNGG 5 cut(s) 263, 406, 1598, 2397, 3022
AflII CTTAAG 1 cut(s) 575
AflIII ACRYGT 3 cut(s) 1504, 2410, 2517
AhdI GACNNNNNGTC 1 cut(s) 2242
AjiI CACGTC 1 cut(s) 2518
AjnI CCWGG 7 cut(s) 675, 733, 1350, 1395, 2286, 2428, 2439
Alw21I GWGCWC 3 cut(s) 81, 2166, 2907
Alw26I GTCTC 3 cut(s) 261, 1326, 2173
Alw44I GTGCAC 1 cut(s) 2162
AlwI GGATC 6 cut(s) 107, 809, 1649, 1662, 1742, 2204
AlwNI CAGNNNCTG 2 cut(s) 1960, 3105
Ama87I CYCGRG 2 cut(s) 80, 2991
Aor13HI TCCGGA 2 cut(s) 2272, 2372
ApaLI GTGCAC 1 cut(s) 2162
ApeKI GCWGC 9 cut(s) 359, 362, 365, 387, 955, 1175, 2494, 2602, 2885
ApoI RAATTY 8 cut(s) 120, 668, 1370, 1376, 1891, 2077, 2260, 2365
AseI ATTAAT 1 cut(s) 1793
Asp700I GAANNNNTTC 3 cut(s) 595, 711, 2412
AspLEI GCGC 3 cut(s) 424, 813, 3034
AsuC2I CCSGG 1 cut(s) 1124
AsuHPI GGTGA 3 cut(s) 284, 731, 2009
AvaI CYCGRG 2 cut(s) 80, 2991
AvaII GGWCC 3 cut(s) 941, 1988, 2283
BaeGI GKGCMC 1 cut(s) 2166
BalI TGGCCA 1 cut(s) 2066
BamHI GGATCC 1 cut(s) 1654
BanII GRGCYC 2 cut(s) 81, 2907
BarI GAAGNNNNNNTAC 2 cut(s) 1790, 1822
BauI CACGAG 2 cut(s) 1115, 1738
BbsI GAAGAC 4 cut(s) 1052, 1216, 2414, 3066
Bbv12I GWGCWC 3 cut(s) 81, 2166, 2907
BbvI GCAGC 9 cut(s) 349, 371, 377, 399, 942, 1162, 2481, 2614, 2872
BccI CCATC 5 cut(s) 179, 1633, 1730, 1897, 2720
BcgI CGANNNNNNTGC 2 cut(s) 3047, 3081
BciT130I CCWGG 7 cut(s) 677, 735, 1352, 1397, 2288, 2430, 2441
BcnI CCSGG 1 cut(s) 1124
BcoDI GTCTC 3 cut(s) 261, 1326, 2173
BfaI CTAG 4 cut(s) 866, 1007, 1152, 1661
BfmI CTRYAG 4 cut(s) 363, 657, 956, 2886
BfrI CTTAAG 1 cut(s) 575
BfuAI ACCTGC 1 cut(s) 551
BglII AGATCT 2 cut(s) 2545, 2812
Bme1390I CCNGG 8 cut(s) 677, 735, 1124, 1352, 1397, 2288, 2430, 2441
Bme18I GGWCC 3 cut(s) 941, 1988, 2283
BmeRI GACNNNNNGTC 1 cut(s) 2242
BmeT110I CYCGRG 2 cut(s) 80, 2991
BmgBI CACGTC 1 cut(s) 2518
BmiI GGNNCC 5 cut(s) 943, 1389, 1656, 2285, 2571
BmrFI CCNGG 8 cut(s) 677, 735, 1124, 1352, 1397, 2288, 2430, 2441
BmrI ACTGGG 1 cut(s) 2684
BmsI GCATC 5 cut(s) 328, 775, 1321, 1866, 2440
BmuI ACTGGG 1 cut(s) 2684
BpiI GAAGAC 4 cut(s) 1052, 1216, 2414, 3066
BpmI CTGGAG 2 cut(s) 1334, 2027
Bpu10I CCTNAGC 1 cut(s) 2588
BpuEI CTTGAG 2 cut(s) 920, 2197
BpuMI CCSGG 1 cut(s) 1124
BsaWI WCCGGW 4 cut(s) 256, 1990, 2272, 2372
BsaXI ACNNNNNCTCC 2 cut(s) 2267, 2297
Bsc4I CCNNNNNNNGG 5 cut(s) 263, 406, 1598, 2397, 3022
Bse118I RCCGGY 1 cut(s) 1390
Bse1I ACTGG 5 cut(s) 1582, 2010, 2454, 2690, 2942
Bse3DI GCAATG 4 cut(s) 98, 375, 795, 1676
BseAI TCCGGA 2 cut(s) 2272, 2372
BseBI CCWGG 7 cut(s) 677, 735, 1352, 1397, 2288, 2430, 2441
BseGI GGATG 6 cut(s) 190, 1199, 1492, 1722, 1880, 2932
BseLI CCNNNNNNNGG 5 cut(s) 263, 406, 1598, 2397, 3022
BseMI GCAATG 4 cut(s) 98, 375, 795, 1676
BseMII CTCAG 7 cut(s) 269, 614, 1395, 1605, 1877, 2481, 2670
BseNI ACTGG 5 cut(s) 1582, 2010, 2454, 2690, 2942
BseRI GAGGAG 2 cut(s) 33, 1631
BseSI GKGCMC 1 cut(s) 2166
BseXI GCAGC 9 cut(s) 349, 371, 377, 399, 942, 1162, 2481, 2614, 2872
BseYI CCCAGC 1 cut(s) 3098
Bsh1236I CGCG 3 cut(s) 813, 1837, 3034
Bsh1285I CGRYCG 2 cut(s) 405, 3038
BsiEI CGRYCG 2 cut(s) 405, 3038
BsiHKAI GWGCWC 3 cut(s) 81, 2166, 2907
BsiHKCI CYCGRG 2 cut(s) 80, 2991
BsiSI CCGG 6 cut(s) 257, 1123, 1391, 1991, 2273, 2373
BslFI GGGAC 3 cut(s) 429, 927, 2269
BslI CCNNNNNNNGG 5 cut(s) 263, 406, 1598, 2397, 3022
BsmAI GTCTC 3 cut(s) 261, 1326, 2173
BsmBI CGTCTC 1 cut(s) 261
BsmFI GGGAC 3 cut(s) 429, 927, 2269
BsoBI CYCGRG 2 cut(s) 80, 2991
Bsp1286I GDGCHC 3 cut(s) 81, 2166, 2907
Bsp13I TCCGGA 2 cut(s) 2272, 2372
Bsp1407I TGTACA 1 cut(s) 831
Bsp19I CCATGG 3 cut(s) 636, 847, 2311
BspACI CCGC 7 cut(s) 177, 700, 729, 1188, 1835, 1837, 1855
BspCNI CTCAG 7 cut(s) 270, 613, 1394, 1606, 1878, 2482, 2671
BspEI TCCGGA 2 cut(s) 2272, 2372
BspFNI CGCG 3 cut(s) 813, 1837, 3034
BspHI TCATGA 1 cut(s) 1744
BspLI GGNNCC 5 cut(s) 943, 1389, 1656, 2285, 2571
BspMAI CTGCAG 3 cut(s) 367, 960, 2890
BspMI ACCTGC 1 cut(s) 551
BspPI GGATC 6 cut(s) 107, 809, 1649, 1662, 1742, 2204
BspQI GCTCTTC 2 cut(s) 767, 2895
BspTI CTTAAG 1 cut(s) 575
BsrDI GCAATG 4 cut(s) 98, 375, 795, 1676
BsrFI RCCGGY 1 cut(s) 1390
BsrGI TGTACA 1 cut(s) 831
BsrI ACTGG 5 cut(s) 1582, 2010, 2454, 2690, 2942
BssAI RCCGGY 1 cut(s) 1390
BssSI CACGAG 2 cut(s) 1115, 1738
BssT1I CCWWGG 5 cut(s) 636, 847, 1108, 1592, 2311
Bst2BI CACGAG 2 cut(s) 1115, 1738
Bst2UI CCWGG 7 cut(s) 677, 735, 1352, 1397, 2288, 2430, 2441
Bst4CI ACNGT 4 cut(s) 230, 272, 2071, 2645
Bst6I CTCTTC 4 cut(s) 216, 281, 767, 2895
BstAFI CTTAAG 1 cut(s) 575
BstAPI GCANNNNNTGC 1 cut(s) 1964
BstAUI TGTACA 1 cut(s) 831
BstC8I GCNNGC 4 cut(s) 1186, 1589, 1965, 2797
BstDEI CTNAG 9 cut(s) 278, 600, 1381, 1614, 1886, 1925, 2490, 2588, 2679
BstDSI CCRYGG 5 cut(s) 636, 847, 1835, 2311, 2630
BstEII GGTNACC 1 cut(s) 737
BstF5I GGATG 6 cut(s) 190, 1199, 1492, 1722, 1880, 2932
BstFNI CGCG 3 cut(s) 813, 1837, 3034
BstHHI GCGC 3 cut(s) 424, 813, 3034
BstMAI GTCTC 3 cut(s) 261, 1326, 2173
BstMCI CGRYCG 2 cut(s) 405, 3038
BstMWI GCNNNNNNNGC 4 cut(s) 365, 1181, 1964, 2894
BstNI CCWGG 7 cut(s) 677, 735, 1352, 1397, 2288, 2430, 2441
BstNSI RCATGY 3 cut(s) 442, 1508, 2231
BstPI GGTNACC 1 cut(s) 737
BstSCI CCNGG 8 cut(s) 675, 733, 1122, 1350, 1395, 2286, 2428, 2439
BstSFI CTRYAG 4 cut(s) 363, 657, 956, 2886
BstSLI GKGCMC 1 cut(s) 2166
BstUI CGCG 3 cut(s) 813, 1837, 3034
BstV1I GCAGC 9 cut(s) 349, 371, 377, 399, 942, 1162, 2481, 2614, 2872
BstV2I GAAGAC 4 cut(s) 1052, 1216, 2414, 3066
BstX2I RGATCY 3 cut(s) 1654, 2545, 2812
BstXI CCANNNNNNTGG 2 cut(s) 1332, 1358
BstYI RGATCY 3 cut(s) 1654, 2545, 2812
BtgI CCRYGG 5 cut(s) 636, 847, 1835, 2311, 2630
BtgZI GCGATG 1 cut(s) 2949
BtrI CACGTC 1 cut(s) 2518
BtsCI GGATG 6 cut(s) 190, 1199, 1492, 1722, 1880, 2932
BtsI GCAGTG 3 cut(s) 93, 715, 1958
BtsIMutI CAGTG 8 cut(s) 93, 715, 1245, 1589, 1958, 2026, 2461, 3009
BveI ACCTGC 1 cut(s) 551
Cac8I GCNNGC 4 cut(s) 1186, 1589, 1965, 2797
CaiI CAGNNNCTG 2 cut(s) 1960, 3105
CciI TCATGA 1 cut(s) 1744
CfoI GCGC 3 cut(s) 424, 813, 3034
Cfr10I RCCGGY 1 cut(s) 1390
Cfr42I CCGCGG 1 cut(s) 1838
CsiI ACCWGGT 3 cut(s) 675, 733, 2439
Csp6I GTAC 6 cut(s) 456, 650, 832, 1807, 2683, 2941
CspCI CAANNNNNGTGG 4 cut(s) 2002, 2037, 2174, 2209
CviQI GTAC 6 cut(s) 456, 650, 832, 1807, 2683, 2941
DdeI CTNAG 9 cut(s) 278, 600, 1381, 1614, 1886, 1925, 2490, 2588, 2679
DraI TTTAAA 1 cut(s) 1375
DriI GACNNNNNGTC 1 cut(s) 2242
EaeI YGGCCR 1 cut(s) 2064
Eam1104I CTCTTC 4 cut(s) 216, 281, 767, 2895
Eam1105I GACNNNNNGTC 1 cut(s) 2242
EarI CTCTTC 4 cut(s) 216, 281, 767, 2895
Ecl136II GAGCTC 2 cut(s) 79, 2905
Eco130I CCWWGG 5 cut(s) 636, 847, 1108, 1592, 2311
Eco24I GRGCYC 2 cut(s) 81, 2907
Eco47I GGWCC 3 cut(s) 941, 1988, 2283
Eco53kI GAGCTC 2 cut(s) 79, 2905
Eco57I CTGAAG 4 cut(s) 171, 1395, 1886, 3048
Eco88I CYCGRG 2 cut(s) 80, 2991
Eco91I GGTNACC 1 cut(s) 737
EcoICRI GAGCTC 2 cut(s) 79, 2905
EcoO109I RGGNCCY 3 cut(s) 941, 974, 2302
EcoO65I GGTNACC 1 cut(s) 737
EcoRI GAATTC 2 cut(s) 1891, 2260
EcoRII CCWGG 7 cut(s) 675, 733, 1350, 1395, 2286, 2428, 2439
EcoT14I CCWWGG 5 cut(s) 636, 847, 1108, 1592, 2311
EcoT22I ATGCAT 2 cut(s) 523, 1474
EcoT38I GRGCYC 2 cut(s) 81, 2907
ErhI CCWWGG 5 cut(s) 636, 847, 1108, 1592, 2311
Esp3I CGTCTC 1 cut(s) 261
FalI AAGNNNNNCTT 2 cut(s) 761, 793
FaqI GGGAC 3 cut(s) 429, 927, 2269
FauI CCCGC 1 cut(s) 1830
FauNDI CATATG 1 cut(s) 1029
FblI GTMKAC 2 cut(s) 2694, 2980
FokI GGATG 6 cut(s) 197, 1206, 1499, 1709, 1887, 2939
FriOI GRGCYC 2 cut(s) 81, 2907
FspBI CTAG 4 cut(s) 866, 1007, 1152, 1661
FspI TGCGCA 1 cut(s) 423
GlaI GCGC 3 cut(s) 423, 812, 3033
GsaI CCCAGC 1 cut(s) 3102
GsuI CTGGAG 2 cut(s) 1334, 2027
HapII CCGG 6 cut(s) 257, 1123, 1391, 1991, 2273, 2373
HhaI GCGC 3 cut(s) 424, 813, 3034
Hin6I GCGC 3 cut(s) 422, 811, 3032
HinP1I GCGC 3 cut(s) 422, 811, 3032
HincII GTYRAC 1 cut(s) 2523
HindII GTYRAC 1 cut(s) 2523
HindIII AAGCTT 4 cut(s) 1811, 1829, 1977, 2036
HinfI GANTC 8 cut(s) 662, 692, 884, 1250, 1400, 2152, 2989, 3041
HpaII CCGG 6 cut(s) 257, 1123, 1391, 1991, 2273, 2373
HphI GGTGA 3 cut(s) 284, 731, 2009
Hpy166II GTNNAC 9 cut(s) 109, 275, 854, 924, 1430, 2164, 2523, 2695, 2981
Hpy8I GTNNAC 9 cut(s) 109, 275, 854, 924, 1430, 2164, 2523, 2695, 2981
HpyCH4III ACNGT 4 cut(s) 230, 272, 2071, 2645
HpyCH4IV ACGT 4 cut(s) 1275, 2410, 2517, 3089
HpyF10VI GCNNNNNNNGC 4 cut(s) 365, 1181, 1964, 2894
HpyF3I CTNAG 9 cut(s) 278, 600, 1381, 1614, 1886, 1925, 2490, 2588, 2679
HpySE526I ACGT 4 cut(s) 1275, 2410, 2517, 3089
HspAI GCGC 3 cut(s) 422, 811, 3032
Kpn2I TCCGGA 2 cut(s) 2272, 2372
KspI CCGCGG 1 cut(s) 1838
LguI GCTCTTC 2 cut(s) 767, 2895
LmnI GCTCC 3 cut(s) 640, 2275, 2599
Lsp1109I GCAGC 9 cut(s) 349, 371, 377, 399, 942, 1162, 2481, 2614, 2872
LweI GCATC 5 cut(s) 328, 775, 1321, 1866, 2440
MabI ACCWGGT 3 cut(s) 675, 733, 2439
MaeI CTAG 4 cut(s) 866, 1007, 1152, 1661
MaeII ACGT 4 cut(s) 1275, 2410, 2517, 3089
MaeIII GTNAC 4 cut(s) 488, 737, 1305, 2485
MflI RGATCY 3 cut(s) 1654, 2545, 2812
MhlI GDGCHC 3 cut(s) 81, 2166, 2907
MlsI TGGCCA 1 cut(s) 2066
MluNI TGGCCA 1 cut(s) 2066
MlyI GAGTC 3 cut(s) 701, 2983, 3050
MmeI TCCRAC 3 cut(s) 2451, 2724, 2839
Mox20I TGGCCA 1 cut(s) 2066
Mph1103I ATGCAT 2 cut(s) 523, 1474
MroI TCCGGA 2 cut(s) 2272, 2372
MroXI GAANNNNTTC 3 cut(s) 595, 711, 2412
MscI TGGCCA 1 cut(s) 2066
MslI CAYNNNNRTG 3 cut(s) 1054, 1330, 2147
Msp20I TGGCCA 1 cut(s) 2066
MspA1I CMGCKG 2 cut(s) 362, 1837
MspCI CTTAAG 1 cut(s) 575
MspI CCGG 6 cut(s) 257, 1123, 1391, 1991, 2273, 2373
MspR9I CCNGG 8 cut(s) 677, 735, 1124, 1352, 1397, 2288, 2430, 2441
MvaI CCWGG 7 cut(s) 677, 735, 1352, 1397, 2288, 2430, 2441
MvnI CGCG 3 cut(s) 813, 1837, 3034
MwoI GCNNNNNNNGC 4 cut(s) 365, 1181, 1964, 2894
NciI CCSGG 1 cut(s) 1124
NcoI CCATGG 3 cut(s) 636, 847, 2311
NdeI CATATG 1 cut(s) 1029
NlaIV GGNNCC 5 cut(s) 943, 1389, 1656, 2285, 2571
NmuCI GTSAC 1 cut(s) 737
NsbI TGCGCA 1 cut(s) 423
NsiI ATGCAT 2 cut(s) 523, 1474
NspI RCATGY 3 cut(s) 442, 1508, 2231
PaeR7I CTCGAG 1 cut(s) 80
PagI TCATGA 1 cut(s) 1744
PciI ACATGT 1 cut(s) 1504
PciSI GCTCTTC 2 cut(s) 767, 2895
PdmI GAANNNNTTC 3 cut(s) 595, 711, 2412
PfeI GAWTC 5 cut(s) 662, 884, 1250, 1400, 2152
PfoI TCCNGGA 1 cut(s) 1350
Ple19I CGATCG 2 cut(s) 405, 3038
PleI GAGTC 3 cut(s) 700, 2983, 3049
PpsI GAGTC 3 cut(s) 700, 2983, 3049
PpuMI RGGWCCY 1 cut(s) 941
PscI ACATGT 1 cut(s) 1504
PshBI ATTAAT 1 cut(s) 1793
Psp124BI GAGCTC 2 cut(s) 81, 2907
Psp5II RGGWCCY 1 cut(s) 941
Psp6I CCWGG 7 cut(s) 675, 733, 1350, 1395, 2286, 2428, 2439
PspEI GGTNACC 1 cut(s) 737
PspFI CCCAGC 1 cut(s) 3098
PspGI CCWGG 7 cut(s) 675, 733, 1350, 1395, 2286, 2428, 2439
PspN4I GGNNCC 5 cut(s) 943, 1389, 1656, 2285, 2571
PspPPI RGGWCCY 1 cut(s) 941
PstI CTGCAG 3 cut(s) 367, 960, 2890
PstNI CAGNNNCTG 2 cut(s) 1960, 3105
PsuI RGATCY 3 cut(s) 1654, 2545, 2812
PvuI CGATCG 2 cut(s) 405, 3038
PvuII CAGCTG 1 cut(s) 362
RsaI GTAC 6 cut(s) 457, 651, 833, 1808, 2684, 2942
RsaNI GTAC 6 cut(s) 456, 650, 832, 1807, 2683, 2941
RseI CAYNNNNRTG 3 cut(s) 1054, 1330, 2147
SacI GAGCTC 2 cut(s) 81, 2907
SacII CCGCGG 1 cut(s) 1838
SapI GCTCTTC 2 cut(s) 767, 2895
SchI GAGTC 3 cut(s) 701, 2983, 3050
ScrFI CCNGG 8 cut(s) 677, 735, 1124, 1352, 1397, 2288, 2430, 2441
SduI GDGCHC 3 cut(s) 81, 2166, 2907
SexAI ACCWGGT 3 cut(s) 675, 733, 2439
SfaNI GCATC 5 cut(s) 328, 775, 1321, 1866, 2440
SfcI CTRYAG 4 cut(s) 363, 657, 956, 2886
Sfr274I CTCGAG 1 cut(s) 80
Sfr303I CCGCGG 1 cut(s) 1838
SgrBI CCGCGG 1 cut(s) 1838
SinI GGWCC 3 cut(s) 941, 1988, 2283
SlaI CTCGAG 1 cut(s) 80
SmiI ATTTAAAT 1 cut(s) 1375
SmiMI CAYNNNNRTG 3 cut(s) 1054, 1330, 2147
SmlI CTYRAG 4 cut(s) 80, 575, 899, 2176
SmoI CTYRAG 4 cut(s) 80, 575, 899, 2176
SsiI CCGC 7 cut(s) 177, 700, 729, 1188, 1835, 1837, 1855
SspI AATATT 1 cut(s) 1073
SspMI CTAG 4 cut(s) 866, 1007, 1152, 1661
SstI GAGCTC 2 cut(s) 81, 2907
StyD4I CCNGG 8 cut(s) 675, 733, 1122, 1350, 1395, 2286, 2428, 2439
StyI CCWWGG 5 cut(s) 636, 847, 1108, 1592, 2311
SwaI ATTTAAAT 1 cut(s) 1375
TaaI ACNGT 4 cut(s) 230, 272, 2071, 2645
TaiI ACGT 4 cut(s) 1278, 2413, 2520, 3092
TaqI TCGA 4 cut(s) 81, 405, 3044, 3057
TatI WGTACW 3 cut(s) 455, 831, 2682
TauI GCSGC 3 cut(s) 180, 702, 731
TfiI GAWTC 5 cut(s) 662, 884, 1250, 1400, 2152
TscAI CASTG 8 cut(s) 100, 722, 1252, 1589, 1965, 2026, 2461, 3016
TseFI GTSAC 1 cut(s) 737
TseI GCWGC 9 cut(s) 359, 362, 365, 387, 955, 1175, 2494, 2602, 2885
Tsp45I GTSAC 1 cut(s) 737
TspGWI ACGGA 3 cut(s) 1715, 2550, 2647
TspRI CASTG 8 cut(s) 100, 722, 1252, 1589, 1965, 2026, 2461, 3016
Vha464I CTTAAG 1 cut(s) 575
VneI GTGCAC 1 cut(s) 2162
VpaK11BI GGWCC 3 cut(s) 941, 1988, 2283
VspI ATTAAT 1 cut(s) 1793
XapI RAATTY 8 cut(s) 120, 668, 1370, 1376, 1891, 2077, 2260, 2365
XbaI TCTAGA 1 cut(s) 1660
XceI RCATGY 3 cut(s) 442, 1508, 2231
XhoI CTCGAG 1 cut(s) 80
XmiI GTMKAC 2 cut(s) 2694, 2980
XmnI GAANNNNTTC 3 cut(s) 595, 711, 2412
XspI CTAG 4 cut(s) 866, 1007, 1152, 1661
Zsp2I ATGCAT 2 cut(s) 523, 1474
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.