pycom03g04160

Belongs to the peptidase M16 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0000891
Physical Location & Seq
Forward (+)
3255155 .. 3262209
7055 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g04160.3

Sequence Viewer

Length: 2541 bp
ATGGCTCGCTGCACTTTCAAATCGGACGATATCACAATAAAGTCGCCGAATGACAGACGATTATACAGATTAATCAAGCTCGAGAATGGCCTCTCGGCATTGCTCGTTCACGATCCTGAGATTTACCCAGAAGGGCCGCCTGATCACTCCAAATCCGTCGAACACAGTGAAGCTGAAGAGGAAGAAGACGAAGATATGGACGACGAGGACGGAGATGAGGGGGAAGACAGTGAAGGAGAAGAGGATGAAGAGGACGATGACGAAGAAGGCGGAGATGGTGAACTGAAGAAGAAGGGGAAGGGTGGAGACTCTCAGACTAAGAAGGCAGCAGCAGCAATGTGTGTGGGAATAGGCAGCTTCTCCGACCCTTTTGAGGCACAAGGGCTTGCACACTTTCTAGAACACATGCTCTTCATGGGGAGTACAGAGTTTCCAGATGAAAATGAGTATGATAGTTACTTGTCCAAGCATGGAGGTTCATCAAATGCATACACAGAAGCAGAGCATACCTGCTACCATTTTGAAGTGAAGCCAGAGTTTCTTAAGGGTTCCTTGAGAAGATTTTCTCAGTTCTTTGTTTCACCTCTAGTGAAAATTGAAGCAATGGAGCGAGAGGTACAGGCTATAGATTCAGAATTTAACCAGGTTCTGCAGAATGATTCTTGCCGCCTTGAACAAATTCAGTGTCATACATCCGCACCCGGTCACCCATTTAATAGGTTCTGTTGGGGAAATAAGAAGAGCTTGGTTGATGCAATGGAAAAAGGGATCAATTTACGAGAACAAATACTAAAGTTGTATAAGGATTATTACCATGGTGGACTGATGAAGCTAGTTGTCATTGGTGGAGAATCTCTTGATTTACTTGAGGATTGGGTTGTGGAATTGTTTGGTAATGTCAAGAAAGGCCCCCAAGTAAAACTGGAGTTCAAGGCGGAAGGTCCTATCTGGAAAGTTGGCAAACTATACAGGCTAGAGGCTGTTAGAGATGTTAACATACTCAACCTTACATGGACATTTCCATGTCTTCATCAAGACTATTTGAAGAAACCAGAAGATTATTTAGCTCATCTACTTGGACATGAGGGCCGGGGAAGTTTGCATTTTTACCTCAAAACCAGAGGGTGGGCAACATCTTTGTCTGCTGGTGTTGGGGACGAGGGGATGCATCGATCTTCTGTGGCTTATGTCTTCCGCATGTCCATACACCTCACTGACTCTGGATTGGAAAAGATTTCCGAAATAATTTGCTATGTCTACCAATACATTAAGTTACTGCATCAAGTGTCTCCACAAGAATGGATATTTAGGGAACTTCAGGATATTGGGAATATGGATTTTAGATTTGCAGAGGAGCAGCCCCAAGATGATTATGCTGCAGAACTTGCAGAAAATTTACTACTGTATCCAGCAGAGAACGTTATTTATGGGGACTATGTGTACAAAATTTGGGATGCAGAATTGATAAAGTATGTTCTTGGTTTCTTCACACCAGAAAACATGAGGGTTGATGTGGTATCAAAGTCCTCAATTAAGTTGAAAGATTTCCATTGCGAGCCTTGGTTTGGGTCACATTATACTGAGGAAGATATATCTCCATCTTTGATGGATATGTGGAAGAATCCCCCAGAAATTGATGACTCATTGCATCTCCCTTCAAAGAATGAGTTCATTCCTAGTGATTTTTCCATCCGCTCTGATAACCTGTGCATTGATCGATCAAATATATCTTATCCAAGATGTATAATTGATGAACCATTAATAAAGTTCTGGTACAAGCTCGATGATTCATTTAAACTTCCACGGGCAAATACATACTTCCGCATCAATCTGAAGGGTGGATATGCTAACCTGAAAAGTTGTGTTTTGACTGAACTATATATTCTCCTTCTTAAGGATGAGCTGAATGAGATTGTGTACCAGGCCATTGTTGCCAAGCTGGAAACTTCTGTCTCTGTGTTCAGTGACAAACTGGAGCTGAAGGTCTATGGATTTAATGATAAACTTCCAGCTCTCTTGTCAAAAGTGTTGGCAACTGCCAAAAGTTTCATGCCAACAGATCGATCGTTTCAAGACCTTGTCGTTAAAGAAGATATGAAGCGAAGGTTAAAGAATACCAATATGAAGCCTCTGAGTCACTCATCATACTTAAGACTGCAAGTTCTGTGTCAAATTTTTTATGATGCAGATGAGAAGTTGCATGTTTTGGATGAATTGTCTATTTCTGATTTGAAGTCATTCATTCCCGAGCTTTGTTCCCAGCTATACATTGAGGGCCTTTGCCATGGAAATTTGTTAGAAGACGAAGCAATTACCCTGTCAAATATATTCAAATCTAATTTTTCCGTACCACCACTGCCTATCGAATTGAGGCATAAAGAGCATGTTATTTGTCTTCCTCCTGGTGCTAACCTGATTAGAGATTCCAACGTGAAGAATAAGTCGGAAACGAACTCTGTGATTGAGCTGTATTTTCAAGTTGAGCAAGAAGCAGGGATTGAGTCAATCCGATTGAAAGCATTGCGGCATCGATCTTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0003674 GO:0003824 GO:0004175 GO:0004222 GO:0005102 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005777 GO:0005782 GO:0005829 GO:0006508 GO:0006518 GO:0006605 GO:0006625 GO:0006807 GO:0006810 GO:0006886 GO:0006996 GO:0007031 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007169 GO:0007275 GO:0007568 GO:0008104 GO:0008144 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0008270 GO:0008286 GO:0008340 GO:0009056 GO:0009057 GO:0009719 GO:0009725 GO:0009893 GO:0009894 GO:0009896 GO:0009986 GO:0009987 GO:0010033 GO:0010243 GO:0010259 GO:0010604 GO:0010815 GO:0010992 GO:0015031 GO:0015833 GO:0016043 GO:0016787 GO:0017046 GO:0017076 GO:0017144 GO:0019222 GO:0019538 GO:0019725 GO:0022607 GO:0023052 GO:0030163 GO:0030554 GO:0031334 GO:0031907 GO:0031974 GO:0032459 GO:0032461 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0032868 GO:0032869 GO:0032870 GO:0033036 GO:0033218 GO:0033365 GO:0034613 GO:0034641 GO:0035639 GO:0036094 GO:0042176 GO:0042221 GO:0042277 GO:0042562 GO:0042579 GO:0042592 GO:0042737 GO:0042802 GO:0042803 GO:0042886 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043171 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043254 GO:0043434 GO:0043559 GO:0043574 GO:0043603 GO:0043933 GO:0044085 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044421 GO:0044422 GO:0044424 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0045732 GO:0046872 GO:0046907 GO:0046914 GO:0046983 GO:0048518 GO:0048522 GO:0048856 GO:0050435 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051259 GO:0051260 GO:0051603 GO:0051641 GO:0051649 GO:0051716 GO:0060255 GO:0065003 GO:0065007 GO:0065008 GO:0070011 GO:0070013 GO:0070727 GO:0070887 GO:0071310 GO:0071375 GO:0071417 GO:0071495 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072662 GO:0072663 GO:0080090 GO:0097159 GO:0097242 GO:0097367 GO:0140030 GO:0140035 GO:0140036 GO:0140096 GO:1901142 GO:1901143 GO:1901265 GO:1901363 GO:1901564 GO:1901565 GO:1901575 GO:1901652 GO:1901653 GO:1901698 GO:1901699 GO:1901700 GO:1901701
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_M16 Peptidase_M16_C Peptidase_M16_M

Protein Analysis

847

Amino Acids

96.98

Weight (kDa)

4.98

Isoelectric Point (pI)

41.85

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 518
AccB7I CCANNNNNTGG 1 cut(s) 1125
AccBSI CCGCTC 1 cut(s) 1695
AccI GTMKAC 1 cut(s) 1257
AciI CCGC 9 cut(s) 137, 270, 667, 696, 935, 1195, 1693, 1822, 2524
AclI AACGTT 1 cut(s) 1419
AclWI GGATC 2 cut(s) 107, 776
AcsI RAATTY 6 cut(s) 635, 678, 1393, 1446, 2172, 2290
AcuI CTGAAG 5 cut(s) 195, 305, 1301, 1853, 2000
AfaI GTAC 6 cut(s) 424, 618, 1442, 1775, 1919, 2349
AfiI CCNNNNNNNGG 4 cut(s) 373, 1125, 1565, 1894
AflII CTTAAG 3 cut(s) 542, 1892, 2149
AjnI CCWGG 3 cut(s) 642, 1920, 2401
Alw26I GTCTC 3 cut(s) 300, 1293, 1957
AlwI GGATC 2 cut(s) 107, 776
AlwNI CAGNNNCTG 1 cut(s) 649
Ama87I CYCGRG 2 cut(s) 80, 2246
AoxI GGCC 6 cut(s) 88, 134, 907, 1087, 1923, 2275
ApeKI GCWGC 7 cut(s) 9, 326, 329, 332, 354, 1357, 1376
ApoI RAATTY 6 cut(s) 635, 678, 1393, 1446, 2172, 2290
ArsI GACNNNNNNTTYG 2 cut(s) 670, 702
AseI ATTAAT 2 cut(s) 71, 1760
Asp700I GAANNNNTTC 5 cut(s) 562, 678, 1544, 1667, 2237
AspS9I GGNCC 5 cut(s) 134, 908, 941, 1087, 2275
AsuC2I CCSGG 2 cut(s) 702, 1091
AsuHPI GGTGA 3 cut(s) 290, 573, 698
AvaI CYCGRG 2 cut(s) 80, 2246
AvaII GGWCC 1 cut(s) 941
BaeI ACNNNNGTAYC 2 cut(s) 1388, 1421
BbsI GAAGAC 6 cut(s) 192, 231, 1019, 1183, 2307, 2387
BbvI GCAGC 6 cut(s) 338, 341, 344, 366, 1363, 1369
BccI CCATC 4 cut(s) 269, 1600, 1606, 1697
BciT130I CCWGG 3 cut(s) 644, 1922, 2403
BciVI GTATCC 1 cut(s) 1416
BclI TGATCA 1 cut(s) 142
BcnI CCSGG 2 cut(s) 702, 1091
BcoDI GTCTC 3 cut(s) 300, 1293, 1957
BfaI CTAG 5 cut(s) 398, 587, 833, 974, 1677
BfmI CTRYAG 3 cut(s) 624, 650, 1377
BfrI CTTAAG 3 cut(s) 542, 1892, 2149
BfuAI ACCTGC 1 cut(s) 518
BfuI GTATCC 1 cut(s) 1416
Bme1390I CCNGG 5 cut(s) 644, 702, 1091, 1922, 2403
Bme18I GGWCC 1 cut(s) 941
BmeT110I CYCGRG 2 cut(s) 80, 2246
BmgT120I GGNCC 5 cut(s) 134, 908, 941, 1087, 2275
BmiI GGNNCC 2 cut(s) 550, 910
BmrFI CCNGG 5 cut(s) 644, 702, 1091, 1922, 2403
BmsI GCATC 9 cut(s) 742, 1155, 1177, 1288, 1444, 1657, 1833, 2173, 2536
BpiI GAAGAC 6 cut(s) 192, 231, 1019, 1183, 2307, 2387
BpmI CTGGAG 2 cut(s) 944, 1994
BpuEI CTTGAG 2 cut(s) 574, 887
BpuMI CCSGG 2 cut(s) 702, 1091
Bsa29I ATCGAT 4 cut(s) 1171, 1717, 2062, 2530
BsaJI CCNNGG 5 cut(s) 814, 1090, 1559, 1802, 2284
Bsc4I CCNNNNNNNGG 4 cut(s) 373, 1125, 1565, 1894
Bse1I ACTGG 2 cut(s) 927, 1977
Bse3DI GCAATG 7 cut(s) 98, 342, 609, 762, 1549, 1643, 2519
BseBI CCWGG 3 cut(s) 644, 1922, 2403
BseCI ATCGAT 4 cut(s) 1171, 1717, 2062, 2530
BseDI CCNNGG 5 cut(s) 814, 1090, 1559, 1802, 2284
BseGI GGATG 7 cut(s) 250, 692, 1170, 1459, 1689, 1903, 2215
BseLI CCNNNNNNNGG 4 cut(s) 373, 1125, 1565, 1894
BseMI GCAATG 7 cut(s) 98, 342, 609, 762, 1549, 1643, 2519
BseMII CTCAG 5 cut(s) 108, 326, 581, 1572, 2123
BseNI ACTGG 2 cut(s) 927, 1977
BseRI GAGGAG 1 cut(s) 1367
BseXI GCAGC 6 cut(s) 338, 341, 344, 366, 1363, 1369
BseYI CCCAGC 1 cut(s) 2259
Bsh1285I CGRYCG 1 cut(s) 2066
BshFI GGCC 6 cut(s) 90, 136, 909, 1089, 1925, 2277
BshVI ATCGAT 4 cut(s) 1171, 1717, 2062, 2530
BsiEI CGRYCG 1 cut(s) 2066
BsiHKCI CYCGRG 2 cut(s) 80, 2246
BsiSI CCGG 2 cut(s) 702, 1090
BslFI GGGAC 2 cut(s) 1169, 1445
BslI CCNNNNNNNGG 4 cut(s) 373, 1125, 1565, 1894
BsmAI GTCTC 3 cut(s) 300, 1293, 1957
BsmFI GGGAC 2 cut(s) 1169, 1445
BsnI GGCC 6 cut(s) 90, 136, 909, 1089, 1925, 2277
BsoBI CYCGRG 2 cut(s) 80, 2246
Bsp1407I TGTACA 1 cut(s) 1440
Bsp143I GATC 9 cut(s) 112, 142, 768, 1172, 1714, 1718, 2059, 2063, 2531
Bsp19I CCATGG 2 cut(s) 814, 2284
BspACI CCGC 9 cut(s) 137, 270, 667, 696, 935, 1195, 1693, 1822, 2524
BspANI GGCC 6 cut(s) 90, 136, 909, 1089, 1925, 2277
BspCNI CTCAG 5 cut(s) 109, 325, 580, 1573, 2124
BspDI ATCGAT 4 cut(s) 1171, 1717, 2062, 2530
BspLI GGNNCC 2 cut(s) 550, 910
BspMAI CTGCAG 2 cut(s) 654, 1381
BspMI ACCTGC 1 cut(s) 518
BspPI GGATC 2 cut(s) 107, 776
BspQI GCTCTTC 2 cut(s) 416, 734
BspTI CTTAAG 3 cut(s) 542, 1892, 2149
BsrBI CCGCTC 1 cut(s) 1695
BsrDI GCAATG 7 cut(s) 98, 342, 609, 762, 1549, 1643, 2519
BsrGI TGTACA 1 cut(s) 1440
BsrI ACTGG 2 cut(s) 927, 1977
BssECI CCNNGG 5 cut(s) 814, 1090, 1559, 1802, 2284
BssMI GATC 9 cut(s) 112, 142, 768, 1172, 1714, 1718, 2059, 2063, 2531
BssT1I CCWWGG 3 cut(s) 814, 1559, 2284
Bst2UI CCWGG 3 cut(s) 644, 1922, 2403
Bst4CI ACNGT 3 cut(s) 167, 230, 1404
Bst6I CTCTTC 5 cut(s) 171, 234, 243, 416, 734
BstAFI CTTAAG 3 cut(s) 542, 1892, 2149
BstAPI GCANNNNNTGC 1 cut(s) 1385
BstAUI TGTACA 1 cut(s) 1440
BstC8I GCNNGC 3 cut(s) 7, 387, 1556
BstDEI CTNAG 6 cut(s) 117, 312, 318, 567, 1581, 2132
BstDSI CCRYGG 3 cut(s) 814, 1802, 2284
BstEII GGTNACC 1 cut(s) 704
BstENI CCTNNNNNAGG 1 cut(s) 1892
BstF5I GGATG 7 cut(s) 250, 692, 1170, 1459, 1689, 1903, 2215
BstKTI GATC 9 cut(s) 115, 145, 771, 1175, 1717, 1721, 2062, 2066, 2534
BstMAI GTCTC 3 cut(s) 300, 1293, 1957
BstMBI GATC 9 cut(s) 112, 142, 768, 1172, 1714, 1718, 2059, 2063, 2531
BstMCI CGRYCG 1 cut(s) 2066
BstMWI GCNNNNNNNGC 4 cut(s) 332, 1385, 1931, 2380
BstNI CCWGG 3 cut(s) 644, 1922, 2403
BstNSI RCATGY 4 cut(s) 409, 1201, 2204, 2387
BstPI GGTNACC 1 cut(s) 704
BstSCI CCNGG 5 cut(s) 642, 700, 1089, 1920, 2401
BstSFI CTRYAG 3 cut(s) 624, 650, 1377
BstV1I GCAGC 6 cut(s) 338, 341, 344, 366, 1363, 1369
BstV2I GAAGAC 6 cut(s) 192, 231, 1019, 1183, 2307, 2387
BstXI CCANNNNNNTGG 1 cut(s) 1299
Bsu15I ATCGAT 4 cut(s) 1171, 1717, 2062, 2530
BsuI GTATCC 1 cut(s) 1416
BsuRI GGCC 6 cut(s) 90, 136, 909, 1089, 1925, 2277
BsuTUI ATCGAT 4 cut(s) 1171, 1717, 2062, 2530
BtgI CCRYGG 3 cut(s) 814, 1802, 2284
BtsCI GGATG 7 cut(s) 250, 692, 1170, 1459, 1689, 1903, 2215
BtsI GCAGTG 1 cut(s) 2354
BtsIMutI CAGTG 6 cut(s) 172, 235, 689, 1212, 1969, 2354
BveI ACCTGC 1 cut(s) 518
Cac8I GCNNGC 3 cut(s) 7, 387, 1556
CaiI CAGNNNCTG 1 cut(s) 649
Cfr13I GGNCC 5 cut(s) 134, 908, 941, 1087, 2275
ClaI ATCGAT 4 cut(s) 1171, 1717, 2062, 2530
CsiI ACCWGGT 1 cut(s) 642
Csp6I GTAC 6 cut(s) 423, 617, 1441, 1774, 1918, 2348
CspCI CAANNNNNGTGG 2 cut(s) 324, 359
CviQI GTAC 6 cut(s) 423, 617, 1441, 1774, 1918, 2348
DdeI CTNAG 6 cut(s) 117, 312, 318, 567, 1581, 2132
DpnI GATC 9 cut(s) 114, 144, 770, 1174, 1716, 1720, 2061, 2065, 2533
DpnII GATC 9 cut(s) 112, 142, 768, 1172, 1714, 1718, 2059, 2063, 2531
DraI TTTAAA 1 cut(s) 1795
Eam1104I CTCTTC 5 cut(s) 171, 234, 243, 416, 734
EarI CTCTTC 5 cut(s) 171, 234, 243, 416, 734
EciI GGCGGA 2 cut(s) 285, 950
Eco130I CCWWGG 3 cut(s) 814, 1559, 2284
Eco32I GATATC 1 cut(s) 31
Eco47I GGWCC 1 cut(s) 941
Eco57I CTGAAG 5 cut(s) 195, 305, 1301, 1853, 2000
Eco88I CYCGRG 2 cut(s) 80, 2246
Eco91I GGTNACC 1 cut(s) 704
EcoNI CCTNNNNNAGG 1 cut(s) 1892
EcoO109I RGGNCCY 3 cut(s) 908, 941, 2275
EcoO65I GGTNACC 1 cut(s) 704
EcoRII CCWGG 3 cut(s) 642, 1920, 2401
EcoRV GATATC 1 cut(s) 31
EcoT14I CCWWGG 3 cut(s) 814, 1559, 2284
EcoT22I ATGCAT 2 cut(s) 490, 1170
ErhI CCWWGG 3 cut(s) 814, 1559, 2284
FalI AAGNNNNNCTT 4 cut(s) 536, 568, 728, 760
FaqI GGGAC 2 cut(s) 1169, 1445
FbaI TGATCA 1 cut(s) 142
FblI GTMKAC 1 cut(s) 1257
FokI GGATG 7 cut(s) 257, 679, 1177, 1466, 1676, 1910, 2222
FspBI CTAG 5 cut(s) 398, 587, 833, 974, 1677
GsaI CCCAGC 1 cut(s) 2263
GsuI CTGGAG 2 cut(s) 944, 1994
HaeIII GGCC 6 cut(s) 90, 136, 909, 1089, 1925, 2277
HapII CCGG 2 cut(s) 702, 1090
HincII GTYRAC 1 cut(s) 994
HindII GTYRAC 1 cut(s) 994
HpaI GTTAAC 1 cut(s) 994
HpaII CCGG 2 cut(s) 702, 1090
HphI GGTGA 3 cut(s) 290, 573, 698
Hpy166II GTNNAC 7 cut(s) 109, 281, 821, 994, 1258, 1441, 1918
Hpy8I GTNNAC 7 cut(s) 109, 281, 821, 994, 1258, 1441, 1918
Hpy99I CGWCG 2 cut(s) 161, 206
HpyCH4III ACNGT 3 cut(s) 167, 230, 1404
HpyCH4IV ACGT 2 cut(s) 1419, 2430
HpyF10VI GCNNNNNNNGC 4 cut(s) 332, 1385, 1931, 2380
HpyF3I CTNAG 6 cut(s) 117, 312, 318, 567, 1581, 2132
HpySE526I ACGT 2 cut(s) 1419, 2430
Ksp22I TGATCA 1 cut(s) 142
KspAI GTTAAC 1 cut(s) 994
Kzo9I GATC 9 cut(s) 112, 142, 768, 1172, 1714, 1718, 2059, 2063, 2531
LguI GCTCTTC 2 cut(s) 416, 734
LmnI GCTCC 3 cut(s) 607, 1354, 1975
Lsp1109I GCAGC 6 cut(s) 338, 341, 344, 366, 1363, 1369
LweI GCATC 9 cut(s) 742, 1155, 1177, 1288, 1444, 1657, 1833, 2173, 2536
MabI ACCWGGT 1 cut(s) 642
MaeI CTAG 5 cut(s) 398, 587, 833, 974, 1677
MaeII ACGT 2 cut(s) 1419, 2430
MaeIII GTNAC 6 cut(s) 455, 704, 1272, 1569, 1964, 2135
MalI GATC 9 cut(s) 114, 144, 770, 1174, 1716, 1720, 2061, 2065, 2533
MbiI CCGCTC 1 cut(s) 1695
MboI GATC 9 cut(s) 112, 142, 768, 1172, 1714, 1718, 2059, 2063, 2531
MlyI GAGTC 5 cut(s) 302, 1211, 1634, 2143, 2510
MmeI TCCRAC 3 cut(s) 387, 2424, 2451
Mph1103I ATGCAT 2 cut(s) 490, 1170
MroXI GAANNNNTTC 5 cut(s) 562, 678, 1544, 1667, 2237
MslI CAYNNNNRTG 2 cut(s) 1021, 1297
MspCI CTTAAG 3 cut(s) 542, 1892, 2149
MspI CCGG 2 cut(s) 702, 1090
MspR9I CCNGG 5 cut(s) 644, 702, 1091, 1922, 2403
MvaI CCWGG 3 cut(s) 644, 1922, 2403
MwoI GCNNNNNNNGC 4 cut(s) 332, 1385, 1931, 2380
NciI CCSGG 2 cut(s) 702, 1091
NcoI CCATGG 2 cut(s) 814, 2284
NdeII GATC 9 cut(s) 112, 142, 768, 1172, 1714, 1718, 2059, 2063, 2531
NlaIV GGNNCC 2 cut(s) 550, 910
NmeAIII GCCGAG 1 cut(s) 74
NmuCI GTSAC 4 cut(s) 704, 1569, 1964, 2135
NsiI ATGCAT 2 cut(s) 490, 1170
NspI RCATGY 4 cut(s) 409, 1201, 2204, 2387
PaeR7I CTCGAG 1 cut(s) 80
PciSI GCTCTTC 2 cut(s) 416, 734
PdmI GAANNNNTTC 5 cut(s) 562, 678, 1544, 1667, 2237
PfeI GAWTC 6 cut(s) 629, 659, 851, 1621, 1787, 2423
PflFI GACNNNGTC 1 cut(s) 2078
PflMI CCANNNNNTGG 1 cut(s) 1125
Ple19I CGATCG 1 cut(s) 2066
PleI GAGTC 5 cut(s) 302, 1211, 1634, 2142, 2509
PpsI GAGTC 5 cut(s) 302, 1211, 1634, 2142, 2509
PpuMI RGGWCCY 1 cut(s) 941
PshBI ATTAAT 2 cut(s) 71, 1760
Psp1406I AACGTT 1 cut(s) 1419
Psp5II RGGWCCY 1 cut(s) 941
Psp6I CCWGG 3 cut(s) 642, 1920, 2401
PspEI GGTNACC 1 cut(s) 704
PspFI CCCAGC 1 cut(s) 2259
PspGI CCWGG 3 cut(s) 642, 1920, 2401
PspN4I GGNNCC 2 cut(s) 550, 910
PspPI GGNCC 5 cut(s) 134, 908, 941, 1087, 2275
PspPPI RGGWCCY 1 cut(s) 941
PstI CTGCAG 2 cut(s) 654, 1381
PstNI CAGNNNCTG 1 cut(s) 649
PsyI GACNNNGTC 1 cut(s) 2078
PvuI CGATCG 1 cut(s) 2066
RsaI GTAC 6 cut(s) 424, 618, 1442, 1775, 1919, 2349
RsaNI GTAC 6 cut(s) 423, 617, 1441, 1774, 1918, 2348
RseI CAYNNNNRTG 2 cut(s) 1021, 1297
SapI GCTCTTC 2 cut(s) 416, 734
Sau3AI GATC 9 cut(s) 112, 142, 768, 1172, 1714, 1718, 2059, 2063, 2531
Sau96I GGNCC 5 cut(s) 134, 908, 941, 1087, 2275
SchI GAGTC 5 cut(s) 302, 1211, 1634, 2143, 2510
ScrFI CCNGG 5 cut(s) 644, 702, 1091, 1922, 2403
SexAI ACCWGGT 1 cut(s) 642
SfaNI GCATC 9 cut(s) 742, 1155, 1177, 1288, 1444, 1657, 1833, 2173, 2536
SfcI CTRYAG 3 cut(s) 624, 650, 1377
Sfr274I CTCGAG 1 cut(s) 80
SinI GGWCC 1 cut(s) 941
SlaI CTCGAG 1 cut(s) 80
SmiMI CAYNNNNRTG 2 cut(s) 1021, 1297
SmlI CTYRAG 6 cut(s) 80, 542, 553, 866, 1892, 2149
SmoI CTYRAG 6 cut(s) 80, 542, 553, 866, 1892, 2149
SsiI CCGC 9 cut(s) 137, 270, 667, 696, 935, 1195, 1693, 1822, 2524
SspMI CTAG 5 cut(s) 398, 587, 833, 974, 1677
StyD4I CCNGG 5 cut(s) 642, 700, 1089, 1920, 2401
StyI CCWWGG 3 cut(s) 814, 1559, 2284
TaaI ACNGT 3 cut(s) 167, 230, 1404
TaiI ACGT 2 cut(s) 1422, 2433
TaqI TCGA 8 cut(s) 81, 159, 1171, 1717, 1782, 2062, 2364, 2530
TatI WGTACW 2 cut(s) 422, 1440
TauI GCSGC 3 cut(s) 139, 669, 2527
TfiI GAWTC 6 cut(s) 629, 659, 851, 1621, 1787, 2423
TscAI CASTG 6 cut(s) 172, 235, 689, 1219, 1969, 2361
TseFI GTSAC 4 cut(s) 704, 1569, 1964, 2135
TseI GCWGC 7 cut(s) 9, 326, 329, 332, 354, 1357, 1376
Tsp45I GTSAC 4 cut(s) 704, 1569, 1964, 2135
TspGWI ACGGA 3 cut(s) 145, 225, 2335
TspRI CASTG 6 cut(s) 172, 235, 689, 1219, 1969, 2361
Tth111I GACNNNGTC 1 cut(s) 2078
Van91I CCANNNNNTGG 1 cut(s) 1125
Vha464I CTTAAG 3 cut(s) 542, 1892, 2149
VpaK11BI GGWCC 1 cut(s) 941
VspI ATTAAT 2 cut(s) 71, 1760
XagI CCTNNNNNAGG 1 cut(s) 1892
XapI RAATTY 6 cut(s) 635, 678, 1393, 1446, 2172, 2290
XbaI TCTAGA 1 cut(s) 397
XceI RCATGY 4 cut(s) 409, 1201, 2204, 2387
XhoI CTCGAG 1 cut(s) 80
XmiI GTMKAC 1 cut(s) 1257
XmnI GAANNNNTTC 5 cut(s) 562, 678, 1544, 1667, 2237
XspI CTAG 5 cut(s) 398, 587, 833, 974, 1677
Zsp2I ATGCAT 2 cut(s) 490, 1170
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.