Rroxscaffold_1G00008380

Belongs to the peptidase M16 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000891
Physical Location & Seq
Forward (+)
10623071 .. 10631586
8516 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00008380.1

Sequence Viewer

Length: 2517 bp
ATGGCTCGCCGGACTTTCTCATCGGACGACGTCGTTTTGAAGTCTCCGAACGATAGGCGACTGTACCGATTGATCAAGCTCGAGAATGGCCTCACTGCATTGCTAGTTCACGATCCTGAGATTGGAGATTCCCAACTCCCCAAGGGCTCTGAGGAAGCTGAGATGGAAGAAGAAGAAGAAGAAGAAGAAGGCATGGAGGAGGATGAGGATGAGGAAGACGACGATGAAGAAGACGAAGATAGTGAAGGAGAGGATGAAGAGATGGATGAAGATGATGAGGGAGAAGATGAAGATGGACTGAAGAAGAAAAAGGAGGGGATTCTCAGACTAAGAAGGTTGGCAGCTGCGGCAATGTGTGTAGGAATAGGCAGCTTCTCTGACCCTCCTGAGGCACCGGGGCTTGCACACTTTCTAGAACACATGCTCTTTATGGGGAGTACAGAATTTCCAGATGAAAATGAGTATGACAGTTACTTGTCCAAGCATGGAGGGTGTTCAAATGCATACACGGAGGTAGAGCATACATGCTACCATTTTGAAGTGAAACGAGAGTTTCTCAAGGAATCTCTTGATGTACTTGAGAATTGGGTTTTGGAATTGTTTGGTAACGTCAAAAAAGGTCCCCAAGTAAATCTGGAATTCAAGGCGGAAGGTCCCATTTGGAAAGCTGGAAAAGTTTACAGGCTAGAGGCTGTTAAAGATGTTCATATACTCCACTTAACATGGACATTGCCATGCCTTCGTCAAGACTATTTGAAGAAATCAGAAGATTATTTATCTCATCTCCTTGGGCATGAGGGCAGGGGAAGTTTGCATTCCCATTTCAAAGTTAAGGGGTGGGCAACATCTCTGGCTGCTGGTGTTGGGGATGACGGGATGCATCGTTCTTCTGTGTCTTATGTCTTTCGCATGGACATTCATCTCACTGACTCTGGATTGGATAAGATTTTTGATATAATTGGCTTGGTCTACCAATACATAAAGTTATTGCATCAAGTGTCCCCACAAGAATGGATATTTAAGGAACTGCAGGATACTGGGAACATGGAATTTAGATTTGCAGAGGAGCAGCCTCAGGATGATTATGCCTCAGAACTTGCAGGAAATTTACTAATTTATGCAGCAGAACATGTTATTTATGGGTCCTATGCATACAAGATTTGGGATGAGGAATCGATCAAATATGTTCTTGATTTCCTTAGACCAGAAAACATGAGGATTGATGTTGTATCAAAGCCCTCATTTAAGTCAGAAGATTTCCAGTGCGAACCTTGGTTTGGGTCACATTATACTGAGGAAGATATATCTCCATCTTTGATAGAGTTGTGGAAGGACCCTCCAGAAATTGATGTTTCATTGCATCTCCCAGAAAAGAATGAGTTCATTCCTACTGATTTTTCCATTCGTTCTGATGATCTTGATACTACAGATACAGCTATTCCAAGATGTATACTGGATGAGCCATTGGTGAAGTTCTGGTACAAACTTGATAATACTTTTAAACTTCCGCGGGCAAATACTTATTTCCGCATCAATCCCAAGGGTGGATATGATAATGTGAAGAGTTGTGTCTTGACTGAACTATATGTATCCCTCCTCAAAGATGAGCTGAATGAGATTGTTTATCAGGCTAGTGTTGCCAAGCTGGAAACTTCGGTTTCTGTATCGACTGACAAGCTGGAGCTAAAGGTCTACGGTTTCAATGACAAGCTTCCAGCTCTGTTGTCAAAGATTCTGAAAACAACCAAAAGTTTCATGCCAACTTCTGATCGTTTTATGGTTATCAAAGAAGATATGGAGCGAAAGTTAAAGAACACCAATATGAAACCTCTGAGTCACTCTACATACTTGAGAGTGCAAGTTTTGTTCCAGAGATTCTATGATGTTGATGAGAAGTTGCATGTTTTAAGTGGACTGTCTATTTCTGATATGAAGTCATTCATTCCCCAACTTTGGTCCCAGGTATACATTGAAGGCCTCTGTCATGGAAATTTGTCAGAAAAAGAAGCAATCAGCCTTTCAGATATATTTAAAACAAATTTTTGTGTACAACCACTTCCAATTGAACTGAGGCGCAGTGAACAGTGTATTTGTCTTCCTCCTTCTGCTAACCTCATTAGAGATGTCAGCGTGAAGAATAAGTCTGAAAAAAATTCAGTGATTGAGCTGTATTTTCAAATTGAGCGGGAATTGAGGATTGAGTCCCCCAGATTGAGAGCCCTGATAGATCTTTTCAATGAAATTGTGGAGGAACCACTTTTCAATCAGCTAAGGTATATGTTTGACTATACAAAACAGGAGGCAGAACGACTCAGGAGCATTCAGAAGGAGGATGTCATCAACTGGTACAAAACATATTTGCAACAATCATCTCCCAAGTGTCGGAGACTTGCAATTCGTGTTTGGGGATGCAACACAGACTTGAAAGAGGCTGAAGCACGGCCAGAGTCTGTACAAGTCATTGAAGACCTTGCAGCCTTTAAGATGTCATCTGAGTTCTATCCAAGCATTTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0003674 GO:0003824 GO:0004175 GO:0004222 GO:0005102 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005777 GO:0005782 GO:0005829 GO:0006508 GO:0006518 GO:0006605 GO:0006625 GO:0006807 GO:0006810 GO:0006886 GO:0006996 GO:0007031 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007169 GO:0007275 GO:0007568 GO:0008104 GO:0008144 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0008270 GO:0008286 GO:0008340 GO:0009056 GO:0009057 GO:0009719 GO:0009725 GO:0009893 GO:0009894 GO:0009896 GO:0009986 GO:0009987 GO:0010033 GO:0010243 GO:0010259 GO:0010604 GO:0010815 GO:0010992 GO:0015031 GO:0015833 GO:0016043 GO:0016787 GO:0017046 GO:0017076 GO:0017144 GO:0019222 GO:0019538 GO:0019725 GO:0022607 GO:0023052 GO:0030163 GO:0030554 GO:0031334 GO:0031907 GO:0031974 GO:0032459 GO:0032461 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0032868 GO:0032869 GO:0032870 GO:0033036 GO:0033218 GO:0033365 GO:0034613 GO:0034641 GO:0035639 GO:0036094 GO:0042176 GO:0042221 GO:0042277 GO:0042562 GO:0042579 GO:0042592 GO:0042737 GO:0042802 GO:0042803 GO:0042886 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043171 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043254 GO:0043434 GO:0043559 GO:0043574 GO:0043603 GO:0043933 GO:0044085 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044421 GO:0044422 GO:0044424 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0045732 GO:0046872 GO:0046907 GO:0046914 GO:0046983 GO:0048518 GO:0048522 GO:0048856 GO:0050435 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051259 GO:0051260 GO:0051603 GO:0051641 GO:0051649 GO:0051716 GO:0060255 GO:0065003 GO:0065007 GO:0065008 GO:0070011 GO:0070013 GO:0070727 GO:0070887 GO:0071310 GO:0071375 GO:0071417 GO:0071495 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072662 GO:0072663 GO:0080090 GO:0097159 GO:0097242 GO:0097367 GO:0140030 GO:0140035 GO:0140036 GO:0140096 GO:1901142 GO:1901143 GO:1901265 GO:1901363 GO:1901564 GO:1901565 GO:1901575 GO:1901652 GO:1901653 GO:1901698 GO:1901699 GO:1901700 GO:1901701
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_M16 Peptidase_M16_C Peptidase_M16_M

Protein Analysis

838

Amino Acids

96.93

Weight (kDa)

4.77

Isoelectric Point (pI)

48.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M16 PF00675 114 - 198 1e-25 Insulinase (Peptidase family M16)
Peptidase_M16_C PF05193 189 - 344 3.1e-09 Peptidase M16 inactive domain
Peptidase_M16_M PF16187 351 - 634 4.3e-91 Middle or third domain of peptidase_M16
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 33
AccB1I GGYRCC 1 cut(s) 391
AccBSI CCGCTC 1 cut(s) 2185
AccI GTMKAC 4 cut(s) 969, 1450, 1692, 1965
AccII CGCG 1 cut(s) 1510
AciI CCGC 6 cut(s) 347, 647, 1508, 1510, 1528, 2185
AclWI GGATC 1 cut(s) 107
AcoI YGGCCR 1 cut(s) 2441
AcsI RAATTY 7 cut(s) 443, 638, 1049, 1105, 1990, 2038, 2152
AcuI CTGAAG 2 cut(s) 320, 2454
AcyI GRCGYC 1 cut(s) 30
AfaI GTAC 7 cut(s) 65, 439, 576, 1481, 2049, 2348, 2454
AfiI CCNNNNNNNGG 6 cut(s) 122, 388, 1277, 1544, 1953, 2382
AflIII ACRYGT 1 cut(s) 1129
AjnI CCWGG 1 cut(s) 1959
AjuI GAANNNNNNNTTGG 4 cut(s) 575, 607, 1260, 1292
AloI GAACNNNNNNTCC 2 cut(s) 868, 900
Alw26I GTCTC 2 cut(s) 48, 2380
AlwI GGATC 1 cut(s) 107
AlwNI CAGNNNCTG 1 cut(s) 2450
Ama87I CYCGRG 1 cut(s) 80
AoxI GGCC 3 cut(s) 88, 1975, 2441
ApeKI GCWGC 7 cut(s) 341, 344, 369, 854, 1069, 1121, 2474
ApoI RAATTY 7 cut(s) 443, 638, 1049, 1105, 1990, 2038, 2152
ArsI GACNNNNNNTTYG 2 cut(s) 2379, 2411
Asp700I GAANNNNTTC 2 cut(s) 1379, 1937
AspLEI GCGC 1 cut(s) 2076
AspS9I GGNCC 5 cut(s) 620, 653, 1143, 1333, 1956
AsuC2I CCSGG 1 cut(s) 396
AsuHPI GGTGA 1 cut(s) 1480
AvaI CYCGRG 1 cut(s) 80
AvaII GGWCC 5 cut(s) 620, 653, 1143, 1333, 1956
AxyI CCTNAGG 2 cut(s) 387, 1074
BanI GGYRCC 1 cut(s) 391
BanII GRGCYC 2 cut(s) 149, 2221
BarI GAAGNNNNNNTAC 4 cut(s) 1463, 1495, 2040, 2072
BbsI GAAGAC 4 cut(s) 222, 237, 2087, 2472
BbvI GCAGC 7 cut(s) 331, 353, 381, 841, 1081, 1133, 2486
BccI CCATC 4 cut(s) 157, 256, 287, 1318
BceAI ACGGC 1 cut(s) 2456
BciT130I CCWGG 1 cut(s) 1961
BciVI GTATCC 2 cut(s) 1027, 1600
BclI TGATCA 1 cut(s) 72
BcnI CCSGG 1 cut(s) 396
BcoDI GTCTC 2 cut(s) 48, 2380
BfaI CTAG 4 cut(s) 104, 413, 686, 1632
BfmI CTRYAG 2 cut(s) 1028, 1425
BfuI GTATCC 2 cut(s) 1027, 1600
BglII AGATCT 1 cut(s) 2227
BisI GCNGC 8 cut(s) 342, 345, 348, 370, 855, 1070, 1122, 2475
BlsI GCNGC 8 cut(s) 343, 346, 349, 371, 856, 1071, 1123, 2476
Bme1390I CCNGG 2 cut(s) 396, 1961
Bme18I GGWCC 5 cut(s) 620, 653, 1143, 1333, 1956
BmeT110I CYCGRG 1 cut(s) 80
BmgT120I GGNCC 5 cut(s) 620, 653, 1143, 1333, 1956
BmiI GGNNCC 7 cut(s) 393, 622, 655, 1144, 1335, 1958, 2253
BmrFI CCNGG 2 cut(s) 396, 1961
BmrI ACTGGG 1 cut(s) 1047
BmsI GCATC 6 cut(s) 867, 889, 1000, 1369, 1539, 2399
BmuI ACTGGG 1 cut(s) 1047
BpiI GAAGAC 4 cut(s) 222, 237, 2087, 2472
BplI GAGNNNNNCTC 2 cut(s) 540, 572
BpmI CTGGAG 2 cut(s) 1323, 1700
Bpu10I CCTNAGC 1 cut(s) 2270
BpuEI CTTGAG 3 cut(s) 542, 599, 1870
BpuMI CCSGG 1 cut(s) 396
Bsa29I ATCGAT 1 cut(s) 1175
BsaHI GRCGYC 1 cut(s) 30
BsaJI CCNNGG 7 cut(s) 141, 395, 787, 1271, 1508, 1539, 1959
BsaXI ACNNNNNCTCC 2 cut(s) 1790, 1820
Bsc4I CCNNNNNNNGG 6 cut(s) 122, 388, 1277, 1544, 1953, 2382
Bse1I ACTGG 4 cut(s) 1042, 1261, 1458, 2348
Bse21I CCTNAGG 2 cut(s) 387, 1074
Bse3DI GCAATG 4 cut(s) 98, 357, 728, 1355
BseBI CCWGG 1 cut(s) 1961
BseCI ATCGAT 1 cut(s) 1175
BseDI CCNNGG 7 cut(s) 141, 395, 787, 1271, 1508, 1539, 1959
BseLI CCNNNNNNNGG 6 cut(s) 122, 388, 1277, 1544, 1953, 2382
BseMI GCAATG 4 cut(s) 98, 357, 728, 1355
BseNI ACTGG 4 cut(s) 1042, 1261, 1458, 2348
BseRI GAGGAG 3 cut(s) 212, 1079, 1586
BseXI GCAGC 7 cut(s) 331, 353, 381, 841, 1081, 1133, 2486
Bsh1236I CGCG 1 cut(s) 1510
BshFI GGCC 3 cut(s) 90, 1977, 2443
BshNI GGYRCC 1 cut(s) 391
BshVI ATCGAT 1 cut(s) 1175
BsiHKCI CYCGRG 1 cut(s) 80
BsiSI CCGG 2 cut(s) 10, 395
BslFI GGGAC 5 cut(s) 606, 639, 985, 1942, 2188
BslI CCNNNNNNNGG 6 cut(s) 122, 388, 1277, 1544, 1953, 2382
BsmAI GTCTC 2 cut(s) 48, 2380
BsmFI GGGAC 5 cut(s) 606, 639, 985, 1942, 2188
BsmI GAATGC 2 cut(s) 814, 2319
BsnI GGCC 3 cut(s) 90, 1977, 2443
BsoBI CYCGRG 1 cut(s) 80
Bsp1286I GDGCHC 2 cut(s) 149, 2221
Bsp1407I TGTACA 2 cut(s) 2047, 2452
Bsp143I GATC 6 cut(s) 72, 112, 1176, 1414, 1768, 2227
BspACI CCGC 6 cut(s) 347, 647, 1508, 1510, 1528, 2185
BspANI GGCC 3 cut(s) 90, 1977, 2443
BspDI ATCGAT 1 cut(s) 1175
BspFNI CGCG 1 cut(s) 1510
BspLI GGNNCC 7 cut(s) 393, 622, 655, 1144, 1335, 1958, 2253
BspMAI CTGCAG 1 cut(s) 1032
BspPI GGATC 1 cut(s) 107
BspT107I GGYRCC 1 cut(s) 391
BsrBI CCGCTC 1 cut(s) 2185
BsrDI GCAATG 4 cut(s) 98, 357, 728, 1355
BsrGI TGTACA 2 cut(s) 2047, 2452
BsrI ACTGG 4 cut(s) 1042, 1261, 1458, 2348
BssECI CCNNGG 7 cut(s) 141, 395, 787, 1271, 1508, 1539, 1959
BssMI GATC 6 cut(s) 72, 112, 1176, 1414, 1768, 2227
BssNAI GTATAC 2 cut(s) 1451, 1966
BssNI GRCGYC 1 cut(s) 30
BssT1I CCWWGG 4 cut(s) 141, 787, 1271, 1539
Bst1107I GTATAC 2 cut(s) 1451, 1966
Bst2UI CCWGG 1 cut(s) 1961
Bst4CI ACNGT 5 cut(s) 63, 470, 1697, 1917, 2085
Bst6I CTCTTC 2 cut(s) 252, 1556
BstACI GRCGYC 1 cut(s) 30
BstAUI TGTACA 2 cut(s) 2047, 2452
BstC8I GCNNGC 3 cut(s) 7, 402, 1512
BstDSI CCRYGG 1 cut(s) 1508
BstFNI CGCG 1 cut(s) 1510
BstHHI GCGC 1 cut(s) 2076
BstKTI GATC 6 cut(s) 75, 115, 1179, 1417, 1771, 2230
BstMAI GTCTC 2 cut(s) 48, 2380
BstMBI GATC 6 cut(s) 72, 112, 1176, 1414, 1768, 2227
BstMWI GCNNNNNNNGC 2 cut(s) 347, 1637
BstNI CCWGG 1 cut(s) 1961
BstNSI RCATGY 4 cut(s) 424, 528, 1133, 1904
BstSCI CCNGG 2 cut(s) 394, 1959
BstSFI CTRYAG 2 cut(s) 1028, 1425
BstUI CGCG 1 cut(s) 1510
BstV1I GCAGC 7 cut(s) 331, 353, 381, 841, 1081, 1133, 2486
BstV2I GAAGAC 4 cut(s) 222, 237, 2087, 2472
BstX2I RGATCY 1 cut(s) 2227
BstXI CCANNNNNNTGG 1 cut(s) 1011
BstYI RGATCY 1 cut(s) 2227
BstZ17I GTATAC 2 cut(s) 1451, 1966
Bsu15I ATCGAT 1 cut(s) 1175
Bsu36I CCTNAGG 2 cut(s) 387, 1074
BsuI GTATCC 2 cut(s) 1027, 1600
BsuRI GGCC 3 cut(s) 90, 1977, 2443
BsuTUI ATCGAT 1 cut(s) 1175
BtgI CCRYGG 1 cut(s) 1508
BtsI GCAGTG 2 cut(s) 93, 2083
BtsIMutI CAGTG 6 cut(s) 93, 924, 1268, 2083, 2090, 2163
Cac8I GCNNGC 3 cut(s) 7, 402, 1512
CaiI CAGNNNCTG 1 cut(s) 2450
CfoI GCGC 1 cut(s) 2076
Cfr13I GGNCC 5 cut(s) 620, 653, 1143, 1333, 1956
Cfr42I CCGCGG 1 cut(s) 1511
ClaI ATCGAT 1 cut(s) 1175
Csp6I GTAC 7 cut(s) 64, 438, 575, 1480, 2048, 2347, 2453
CviQI GTAC 7 cut(s) 64, 438, 575, 1480, 2048, 2347, 2453
DpnI GATC 6 cut(s) 74, 114, 1178, 1416, 1770, 2229
DpnII GATC 6 cut(s) 72, 112, 1176, 1414, 1768, 2227
DraI TTTAAA 2 cut(s) 1501, 2032
EaeI YGGCCR 1 cut(s) 2441
Eam1104I CTCTTC 2 cut(s) 252, 1556
EarI CTCTTC 2 cut(s) 252, 1556
EciI GGCGGA 1 cut(s) 662
Eco130I CCWWGG 4 cut(s) 141, 787, 1271, 1539
Eco147I AGGCCT 1 cut(s) 1977
Eco24I GRGCYC 2 cut(s) 149, 2221
Eco47I GGWCC 5 cut(s) 620, 653, 1143, 1333, 1956
Eco57I CTGAAG 2 cut(s) 320, 2454
Eco81I CCTNAGG 2 cut(s) 387, 1074
Eco88I CYCGRG 1 cut(s) 80
EcoO109I RGGNCCY 4 cut(s) 620, 653, 1143, 1333
EcoRI GAATTC 1 cut(s) 638
EcoRII CCWGG 1 cut(s) 1959
EcoT14I CCWWGG 4 cut(s) 141, 787, 1271, 1539
EcoT22I ATGCAT 3 cut(s) 505, 882, 1153
EcoT38I GRGCYC 2 cut(s) 149, 2221
ErhI CCWWGG 4 cut(s) 141, 787, 1271, 1539
FaqI GGGAC 5 cut(s) 606, 639, 985, 1942, 2188
FauI CCCGC 2 cut(s) 1503, 2178
FbaI TGATCA 1 cut(s) 72
FblI GTMKAC 4 cut(s) 969, 1450, 1692, 1965
Fnu4HI GCNGC 8 cut(s) 342, 345, 348, 370, 855, 1070, 1122, 2475
FriOI GRGCYC 2 cut(s) 149, 2221
Fsp4HI GCNGC 8 cut(s) 342, 345, 348, 370, 855, 1070, 1122, 2475
FspBI CTAG 4 cut(s) 104, 413, 686, 1632
GlaI GCGC 1 cut(s) 2075
GluI GCNGC 8 cut(s) 342, 345, 348, 370, 855, 1070, 1122, 2475
GsuI CTGGAG 2 cut(s) 1323, 1700
HaeIII GGCC 3 cut(s) 90, 1977, 2443
HapII CCGG 2 cut(s) 10, 395
HhaI GCGC 1 cut(s) 2076
Hin1I GRCGYC 1 cut(s) 30
Hin6I GCGC 1 cut(s) 2074
HinP1I GCGC 1 cut(s) 2074
HindIII AAGCTT 1 cut(s) 1709
HpaII CCGG 2 cut(s) 10, 395
HphI GGTGA 1 cut(s) 1480
Hpy166II GTNNAC 9 cut(s) 109, 679, 970, 1451, 1693, 1913, 1966, 2048, 2081
Hpy8I GTNNAC 9 cut(s) 109, 679, 970, 1451, 1693, 1913, 1966, 2048, 2081
Hpy99I CGWCG 3 cut(s) 32, 35, 224
HpyAV CCTTC 9 cut(s) 182, 239, 327, 644, 749, 1324, 1967, 2112, 2320
HpyCH4III ACNGT 5 cut(s) 63, 470, 1697, 1917, 2085
HpyCH4IV ACGT 2 cut(s) 30, 609
HpyF10VI GCNNNNNNNGC 2 cut(s) 347, 1637
HpySE526I ACGT 2 cut(s) 30, 609
Hsp92I GRCGYC 1 cut(s) 30
HspAI GCGC 1 cut(s) 2074
Ksp22I TGATCA 1 cut(s) 72
KspI CCGCGG 1 cut(s) 1511
Kzo9I GATC 6 cut(s) 72, 112, 1176, 1414, 1768, 2227
LmnI GCTCC 4 cut(s) 1066, 1681, 1798, 2316
Lsp1109I GCAGC 7 cut(s) 331, 353, 381, 841, 1081, 1133, 2486
LweI GCATC 6 cut(s) 867, 889, 1000, 1369, 1539, 2399
MaeI CTAG 4 cut(s) 104, 413, 686, 1632
MaeII ACGT 2 cut(s) 30, 609
MaeIII GTNAC 4 cut(s) 470, 605, 1281, 1835
MalI GATC 6 cut(s) 74, 114, 1178, 1416, 1770, 2229
MbiI CCGCTC 1 cut(s) 2185
MboI GATC 6 cut(s) 72, 112, 1176, 1414, 1768, 2227
MfeI CAATTG 1 cut(s) 2061
MflI RGATCY 1 cut(s) 2227
MhlI GDGCHC 2 cut(s) 149, 2221
MlyI GAGTC 5 cut(s) 923, 1843, 2210, 2304, 2456
MmeI TCCRAC 1 cut(s) 2363
Mph1103I ATGCAT 3 cut(s) 505, 882, 1153
MroXI GAANNNNTTC 2 cut(s) 1379, 1937
MslI CAYNNNNRTG 3 cut(s) 733, 1009, 1820
MspA1I CMGCKG 2 cut(s) 344, 1510
MspI CCGG 2 cut(s) 10, 395
MspR9I CCNGG 2 cut(s) 396, 1961
MunI CAATTG 1 cut(s) 2061
Mva1269I GAATGC 2 cut(s) 814, 2319
MvaI CCWGG 1 cut(s) 1961
MvnI CGCG 1 cut(s) 1510
MwoI GCNNNNNNNGC 2 cut(s) 347, 1637
NciI CCSGG 1 cut(s) 396
NdeII GATC 6 cut(s) 72, 112, 1176, 1414, 1768, 2227
NlaIV GGNNCC 7 cut(s) 393, 622, 655, 1144, 1335, 1958, 2253
NmuCI GTSAC 2 cut(s) 1281, 1835
NsiI ATGCAT 3 cut(s) 505, 882, 1153
NspI RCATGY 4 cut(s) 424, 528, 1133, 1904
PaeR7I CTCGAG 1 cut(s) 80
PceI AGGCCT 1 cut(s) 1977
PciI ACATGT 1 cut(s) 1129
PctI GAATGC 2 cut(s) 814, 2319
PdmI GAANNNNTTC 2 cut(s) 1379, 1937
PfeI GAWTC 6 cut(s) 128, 319, 563, 1172, 1732, 1875
PflFI GACNNNGTC 1 cut(s) 29
PkrI GCNGC 8 cut(s) 343, 346, 349, 371, 856, 1071, 1123, 2476
PleI GAGTC 5 cut(s) 923, 1842, 2209, 2304, 2455
PpsI GAGTC 5 cut(s) 923, 1842, 2209, 2304, 2455
PpuMI RGGWCCY 4 cut(s) 620, 653, 1143, 1333
PscI ACATGT 1 cut(s) 1129
Psp5II RGGWCCY 4 cut(s) 620, 653, 1143, 1333
Psp6I CCWGG 1 cut(s) 1959
PspGI CCWGG 1 cut(s) 1959
PspN4I GGNNCC 7 cut(s) 393, 622, 655, 1144, 1335, 1958, 2253
PspPI GGNCC 5 cut(s) 620, 653, 1143, 1333, 1956
PspPPI RGGWCCY 4 cut(s) 620, 653, 1143, 1333
PstI CTGCAG 1 cut(s) 1032
PstNI CAGNNNCTG 1 cut(s) 2450
PsuI RGATCY 1 cut(s) 2227
PsyI GACNNNGTC 1 cut(s) 29
PvuII CAGCTG 1 cut(s) 344
RsaI GTAC 7 cut(s) 65, 439, 576, 1481, 2049, 2348, 2454
RsaNI GTAC 7 cut(s) 64, 438, 575, 1480, 2048, 2347, 2453
RseI CAYNNNNRTG 3 cut(s) 733, 1009, 1820
SacII CCGCGG 1 cut(s) 1511
SatI GCNGC 8 cut(s) 342, 345, 348, 370, 855, 1070, 1122, 2475
Sau3AI GATC 6 cut(s) 72, 112, 1176, 1414, 1768, 2227
Sau96I GGNCC 5 cut(s) 620, 653, 1143, 1333, 1956
SchI GAGTC 5 cut(s) 923, 1843, 2210, 2304, 2456
ScrFI CCNGG 2 cut(s) 396, 1961
SduI GDGCHC 2 cut(s) 149, 2221
SfaNI GCATC 6 cut(s) 867, 889, 1000, 1369, 1539, 2399
SfcI CTRYAG 2 cut(s) 1028, 1425
Sfr274I CTCGAG 1 cut(s) 80
Sfr303I CCGCGG 1 cut(s) 1511
SgrBI CCGCGG 1 cut(s) 1511
SinI GGWCC 5 cut(s) 620, 653, 1143, 1333, 1956
SlaI CTCGAG 1 cut(s) 80
SmiMI CAYNNNNRTG 3 cut(s) 733, 1009, 1820
SmlI CTYRAG 4 cut(s) 80, 557, 578, 1849
SmoI CTYRAG 4 cut(s) 80, 557, 578, 1849
SseBI AGGCCT 1 cut(s) 1977
SsiI CCGC 6 cut(s) 347, 647, 1508, 1510, 1528, 2185
SspMI CTAG 4 cut(s) 104, 413, 686, 1632
StuI AGGCCT 1 cut(s) 1977
StyD4I CCNGG 2 cut(s) 394, 1959
StyI CCWWGG 4 cut(s) 141, 787, 1271, 1539
TaaI ACNGT 5 cut(s) 63, 470, 1697, 1917, 2085
TaiI ACGT 2 cut(s) 33, 612
TaqI TCGA 3 cut(s) 81, 1175, 1667
TatI WGTACW 4 cut(s) 437, 574, 2047, 2452
TauI GCSGC 1 cut(s) 350
TfiI GAWTC 6 cut(s) 128, 319, 563, 1172, 1732, 1875
TscAI CASTG 6 cut(s) 100, 931, 1268, 2083, 2090, 2163
TseFI GTSAC 2 cut(s) 1281, 1835
TseI GCWGC 7 cut(s) 341, 344, 369, 854, 1069, 1121, 2474
Tsp45I GTSAC 2 cut(s) 1281, 1835
TspGWI ACGGA 1 cut(s) 524
TspRI CASTG 6 cut(s) 100, 931, 1268, 2083, 2090, 2163
Tth111I GACNNNGTC 1 cut(s) 29
VpaK11BI GGWCC 5 cut(s) 620, 653, 1143, 1333, 1956
XapI RAATTY 7 cut(s) 443, 638, 1049, 1105, 1990, 2038, 2152
XbaI TCTAGA 1 cut(s) 412
XceI RCATGY 4 cut(s) 424, 528, 1133, 1904
XhoI CTCGAG 1 cut(s) 80
XmiI GTMKAC 4 cut(s) 969, 1450, 1692, 1965
XmnI GAANNNNTTC 2 cut(s) 1379, 1937
XspI CTAG 4 cut(s) 104, 413, 686, 1632
ZraI GACGTC 1 cut(s) 31
Zsp2I ATGCAT 3 cut(s) 505, 882, 1153
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.