Rh1DG140200

Belongs to the peptidase M16 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0000891
Physical Location & Seq
Reverse (-)
29672525 .. 29680892
8368 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG140200.1

Sequence Viewer

Length: 354 bp
ATGCCAGATTTGAGCAGAAAGTCGAGGTCCTGGAACTGCGAGGGCCTTGAGGCACTGGAGAGTGGAGAGGAGGTTGGGGAGGAGAGTGTAGAGAAAGGGTTCTCAGCCCAGTGCATGGAGTCAGAGATGTTAGAGAAGGAGGTCTGGTGGGAGGCCAGCTTGGAGTGGAAGGATCGAACGGCAGCAGCAGCAATGTGTGTAGGAATAGGCAGCTTCTCTGACCCTCCTGAGGCACAGGGGCTTGCACACTTTCTAGAACACATGCTTTTCATGGGGAGTACAGAATTTCCAGATGAAAATGAGTTAGTTACTTATCCAAGCACGGAGGGTGTTCAAATCCATATGCAGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0003674 GO:0003824 GO:0004175 GO:0004222 GO:0005102 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005777 GO:0005782 GO:0005829 GO:0006508 GO:0006518 GO:0006605 GO:0006625 GO:0006807 GO:0006810 GO:0006886 GO:0006996 GO:0007031 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007169 GO:0007275 GO:0007568 GO:0008104 GO:0008144 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0008270 GO:0008286 GO:0008340 GO:0009056 GO:0009057 GO:0009719 GO:0009725 GO:0009893 GO:0009894 GO:0009896 GO:0009986 GO:0009987 GO:0010033 GO:0010243 GO:0010259 GO:0010604 GO:0010815 GO:0010992 GO:0015031 GO:0015833 GO:0016043 GO:0016787 GO:0017046 GO:0017076 GO:0017144 GO:0019222 GO:0019538 GO:0019725 GO:0022607 GO:0023052 GO:0030163 GO:0030554 GO:0031334 GO:0031907 GO:0031974 GO:0032459 GO:0032461 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0032868 GO:0032869 GO:0032870 GO:0033036 GO:0033218 GO:0033365 GO:0034613 GO:0034641 GO:0035639 GO:0036094 GO:0042176 GO:0042221 GO:0042277 GO:0042562 GO:0042579 GO:0042592 GO:0042737 GO:0042802 GO:0042803 GO:0042886 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043171 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043254 GO:0043434 GO:0043559 GO:0043574 GO:0043603 GO:0043933 GO:0044085 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044421 GO:0044422 GO:0044424 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0045732 GO:0046872 GO:0046907 GO:0046914 GO:0046983 GO:0048518 GO:0048522 GO:0048856 GO:0050435 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051259 GO:0051260 GO:0051603 GO:0051641 GO:0051649 GO:0051716 GO:0060255 GO:0065003 GO:0065007 GO:0065008 GO:0070011 GO:0070013 GO:0070727 GO:0070887 GO:0071310 GO:0071375 GO:0071417 GO:0071495 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072662 GO:0072663 GO:0080090 GO:0097159 GO:0097242 GO:0097367 GO:0140030 GO:0140035 GO:0140036 GO:0140096 GO:1901142 GO:1901143 GO:1901265 GO:1901363 GO:1901564 GO:1901565 GO:1901575 GO:1901652 GO:1901653 GO:1901698 GO:1901699 GO:1901700 GO:1901701
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_M16 Peptidase_M16_C Peptidase_M16_M

Protein Analysis

117

Amino Acids

13.12

Weight (kDa)

4.32

Isoelectric Point (pI)

35.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M16 PF00675 57 - 112 8.3e-14 Insulinase (Peptidase family M16)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 115
AclWI GGATC 1 cut(s) 180
AcsI RAATTY 1 cut(s) 284
AfaI GTAC 1 cut(s) 280
AfiI CCNNNNNNNGG 2 cut(s) 115, 229
AgsI TTSAA 1 cut(s) 335
AjnI CCWGG 1 cut(s) 29
AluBI AGCT 2 cut(s) 159, 213
AluI AGCT 2 cut(s) 159, 213
AlwI GGATC 1 cut(s) 180
AoxI GGCC 2 cut(s) 43, 153
ApeKI GCWGC 4 cut(s) 182, 185, 188, 210
ApoI RAATTY 1 cut(s) 284
Asp700I GAANNNNTTC 1 cut(s) 98
AspS9I GGNCC 2 cut(s) 27, 43
AvaII GGWCC 1 cut(s) 27
AxyI CCTNAGG 1 cut(s) 228
BbvI GCAGC 4 cut(s) 194, 197, 200, 222
BceAI ACGGC 1 cut(s) 195
BciT130I CCWGG 1 cut(s) 31
BfaI CTAG 1 cut(s) 254
BisI GCNGC 4 cut(s) 183, 186, 189, 211
BlsI GCNGC 4 cut(s) 184, 187, 190, 212
Bme1390I CCNGG 1 cut(s) 31
Bme18I GGWCC 1 cut(s) 27
BmgT120I GGNCC 2 cut(s) 27, 43
BmrFI CCNGG 1 cut(s) 31
BmrI ACTGGG 1 cut(s) 103
BmuI ACTGGG 1 cut(s) 103
BpmI CTGGAG 1 cut(s) 77
BpuEI CTTGAG 1 cut(s) 68
BsaXI ACNNNNNCTCC 4 cut(s) 57, 87, 131, 161
Bsc4I CCNNNNNNNGG 2 cut(s) 115, 229
Bse1I ACTGG 2 cut(s) 60, 109
Bse21I CCTNAGG 1 cut(s) 228
Bse3DI GCAATG 1 cut(s) 198
BseBI CCWGG 1 cut(s) 31
BseLI CCNNNNNNNGG 2 cut(s) 115, 229
BseMI GCAATG 1 cut(s) 198
BseMII CTCAG 2 cut(s) 117, 219
BseNI ACTGG 2 cut(s) 60, 109
BseRI GAGGAG 2 cut(s) 83, 95
BseXI GCAGC 4 cut(s) 194, 197, 200, 222
BshFI GGCC 2 cut(s) 45, 155
BslI CCNNNNNNNGG 2 cut(s) 115, 229
BsnI GGCC 2 cut(s) 45, 155
Bsp143I GATC 1 cut(s) 172
BspANI GGCC 2 cut(s) 45, 155
BspCNI CTCAG 2 cut(s) 116, 220
BspPI GGATC 1 cut(s) 180
BsrDI GCAATG 1 cut(s) 198
BsrI ACTGG 2 cut(s) 60, 109
BssMI GATC 1 cut(s) 172
Bst2UI CCWGG 1 cut(s) 31
BstC8I GCNNGC 2 cut(s) 157, 243
BstDEI CTNAG 2 cut(s) 103, 228
BstKTI GATC 1 cut(s) 175
BstMBI GATC 1 cut(s) 172
BstMWI GCNNNNNNNGC 1 cut(s) 188
BstNI CCWGG 1 cut(s) 31
BstNSI RCATGY 1 cut(s) 265
BstSCI CCNGG 1 cut(s) 29
BstV1I GCAGC 4 cut(s) 194, 197, 200, 222
Bsu36I CCTNAGG 1 cut(s) 228
BsuRI GGCC 2 cut(s) 45, 155
BtsIMutI CAGTG 2 cut(s) 53, 116
Cac8I GCNNGC 2 cut(s) 157, 243
Cfr13I GGNCC 2 cut(s) 27, 43
Csp6I GTAC 1 cut(s) 279
CviAII CATG 3 cut(s) 115, 262, 271
CviJI RGCY 6 cut(s) 45, 107, 155, 159, 213, 241
CviKI_1 RGCY 6 cut(s) 45, 107, 155, 159, 213, 241
CviQI GTAC 1 cut(s) 279
DdeI CTNAG 2 cut(s) 103, 228
DpnI GATC 1 cut(s) 174
DpnII GATC 1 cut(s) 172
Eco47I GGWCC 1 cut(s) 27
Eco81I CCTNAGG 1 cut(s) 228
EcoO109I RGGNCCY 2 cut(s) 27, 43
EcoRII CCWGG 1 cut(s) 29
FaeI CATG 3 cut(s) 118, 265, 274
FaiI YATR 5 cut(s) 116, 263, 272, 342, 344
FatI CATG 3 cut(s) 114, 261, 270
FauNDI CATATG 1 cut(s) 342
Fnu4HI GCNGC 4 cut(s) 183, 186, 189, 211
Fsp4HI GCNGC 4 cut(s) 183, 186, 189, 211
FspBI CTAG 1 cut(s) 254
GluI GCNGC 4 cut(s) 183, 186, 189, 211
GsuI CTGGAG 1 cut(s) 77
HaeIII GGCC 2 cut(s) 45, 155
Hin1II CATG 3 cut(s) 118, 265, 274
HinfI GANTC 1 cut(s) 119
Hpy188I TCNGA 2 cut(s) 124, 220
Hpy188III TCNNGA 3 cut(s) 227, 254, 290
HpyAV CCTTC 2 cut(s) 130, 163
HpyCH4V TGCA 3 cut(s) 114, 245, 346
HpyF10VI GCNNNNNNNGC 1 cut(s) 188
HpyF3I CTNAG 2 cut(s) 103, 228
Hsp92II CATG 3 cut(s) 118, 265, 274
Kzo9I GATC 1 cut(s) 172
Lsp1109I GCAGC 4 cut(s) 194, 197, 200, 222
MaeI CTAG 1 cut(s) 254
MaeIII GTNAC 1 cut(s) 307
MalI GATC 1 cut(s) 174
MboI GATC 1 cut(s) 172
MluCI AATT 1 cut(s) 284
MlyI GAGTC 1 cut(s) 128
MroXI GAANNNNTTC 1 cut(s) 98
MspR9I CCNGG 1 cut(s) 31
MvaI CCWGG 1 cut(s) 31
MwoI GCNNNNNNNGC 1 cut(s) 188
NdeI CATATG 1 cut(s) 342
NdeII GATC 1 cut(s) 172
NlaIII CATG 3 cut(s) 118, 265, 274
NspI RCATGY 1 cut(s) 265
PdmI GAANNNNTTC 1 cut(s) 98
PflMI CCANNNNNTGG 1 cut(s) 115
PfoI TCCNGGA 1 cut(s) 29
PkrI GCNGC 4 cut(s) 184, 187, 190, 212
PleI GAGTC 1 cut(s) 127
PpsI GAGTC 1 cut(s) 127
PpuMI RGGWCCY 1 cut(s) 27
Psp5II RGGWCCY 1 cut(s) 27
Psp6I CCWGG 1 cut(s) 29
PspGI CCWGG 1 cut(s) 29
PspPI GGNCC 2 cut(s) 27, 43
PspPPI RGGWCCY 1 cut(s) 27
RsaI GTAC 1 cut(s) 280
RsaNI GTAC 1 cut(s) 279
SatI GCNGC 4 cut(s) 183, 186, 189, 211
Sau3AI GATC 1 cut(s) 172
Sau96I GGNCC 2 cut(s) 27, 43
SchI GAGTC 1 cut(s) 128
ScrFI CCNGG 1 cut(s) 31
SetI ASST 5 cut(s) 29, 75, 144, 161, 215
SinI GGWCC 1 cut(s) 27
SmlI CTYRAG 1 cut(s) 47
SmoI CTYRAG 1 cut(s) 47
Sse9I AATT 1 cut(s) 284
SspMI CTAG 1 cut(s) 254
StyD4I CCNGG 1 cut(s) 29
TaqI TCGA 2 cut(s) 23, 175
TasI AATT 1 cut(s) 284
TatI WGTACW 1 cut(s) 278
TscAI CASTG 2 cut(s) 60, 116
TseI GCWGC 4 cut(s) 182, 185, 188, 210
TspDTI ATGAA 2 cut(s) 259, 309
TspGWI ACGGA 1 cut(s) 338
TspRI CASTG 2 cut(s) 60, 116
Van91I CCANNNNNTGG 1 cut(s) 115
VpaK11BI GGWCC 1 cut(s) 27
XapI RAATTY 1 cut(s) 284
XbaI TCTAGA 1 cut(s) 253
XceI RCATGY 1 cut(s) 265
XmnI GAANNNNTTC 1 cut(s) 98
XspI CTAG 1 cut(s) 254
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.