Belongs to the peptidase M16 family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 405 |
| Acc36I | ACCTGC | 1 cut(s) | 533 |
| AccB1I | GGYRCC | 1 cut(s) | 563 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1140 |
| AccBSI | CCGCTC | 1 cut(s) | 1710 |
| AccI | GTMKAC | 1 cut(s) | 1272 |
| AccII | CGCG | 1 cut(s) | 1472 |
| AciI | CCGC | 8 cut(s) | 137, 281, 682, 711, 1210, 1472, 1708, 1837 |
| AclI | AACGTT | 1 cut(s) | 1434 |
| AclWI | GGATC | 4 cut(s) | 107, 791, 1699, 1724 |
| AcsI | RAATTY | 4 cut(s) | 650, 693, 1408, 1811 |
| AcuI | CTGAAG | 4 cut(s) | 195, 323, 1868, 1889 |
| AfaI | GTAC | 8 cut(s) | 155, 439, 633, 815, 879, 1280, 1457, 1790 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 1140, 1580, 1909 |
| AflII | CTTAAG | 2 cut(s) | 557, 1907 |
| AgsI | TTSAA | 9 cut(s) | 19, 389, 539, 614, 689, 946, 1060, 1334, 1674 |
| AjnI | CCWGG | 2 cut(s) | 657, 715 |
| AluBI | AGCT | 9 cut(s) | 79, 173, 372, 638, 759, 847, 1082, 1795, 1958 |
| AluI | AGCT | 9 cut(s) | 79, 173, 372, 638, 759, 847, 1082, 1795, 1958 |
| Alw21I | GWGCWC | 2 cut(s) | 81, 165 |
| Alw26I | GTCTC | 1 cut(s) | 1308 |
| AlwI | GGATC | 4 cut(s) | 107, 791, 1699, 1724 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 664 |
| Ama87I | CYCGRG | 1 cut(s) | 80 |
| AoxI | GGCC | 4 cut(s) | 88, 134, 922, 1102 |
| ApeKI | GCWGC | 6 cut(s) | 9, 341, 344, 347, 1372, 1391 |
| ApoI | RAATTY | 4 cut(s) | 650, 693, 1408, 1811 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 685, 717 |
| Asp700I | GAANNNNTTC | 3 cut(s) | 577, 693, 1682 |
| AspLEI | GCGC | 2 cut(s) | 406, 1918 |
| AspS9I | GGNCC | 4 cut(s) | 134, 923, 956, 1102 |
| AsuC2I | CCSGG | 1 cut(s) | 1106 |
| AsuHPI | GGTGA | 3 cut(s) | 308, 588, 713 |
| AsuII | TTCGAA | 1 cut(s) | 1254 |
| AvaI | CYCGRG | 1 cut(s) | 80 |
| AvaII | GGWCC | 1 cut(s) | 956 |
| BaeI | ACNNNNGTAYC | 3 cut(s) | 1403, 1436, 1954 |
| BanI | GGYRCC | 1 cut(s) | 563 |
| BanII | GRGCYC | 1 cut(s) | 81 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 1772, 1804 |
| BbsI | GAAGAC | 3 cut(s) | 234, 1034, 1198 |
| Bbv12I | GWGCWC | 2 cut(s) | 81, 165 |
| BbvI | GCAGC | 5 cut(s) | 353, 356, 359, 1378, 1384 |
| BccI | CCATC | 5 cut(s) | 206, 287, 613, 1615, 1621 |
| BceAI | ACGGC | 1 cut(s) | 103 |
| BciT130I | CCWGG | 2 cut(s) | 659, 717 |
| BciVI | GTATCC | 1 cut(s) | 1431 |
| BcnI | CCSGG | 1 cut(s) | 1106 |
| BcoDI | GTCTC | 1 cut(s) | 1308 |
| BfaI | CTAG | 5 cut(s) | 413, 602, 848, 989, 1692 |
| BfmI | CTRYAG | 3 cut(s) | 639, 665, 1392 |
| BfrI | CTTAAG | 2 cut(s) | 557, 1907 |
| BfuAI | ACCTGC | 1 cut(s) | 533 |
| BfuI | GTATCC | 1 cut(s) | 1431 |
| BisI | GCNGC | 8 cut(s) | 10, 137, 342, 345, 348, 682, 1373, 1392 |
| BlsI | GCNGC | 8 cut(s) | 11, 138, 343, 346, 349, 683, 1374, 1393 |
| Bme1390I | CCNGG | 3 cut(s) | 659, 717, 1106 |
| Bme18I | GGWCC | 1 cut(s) | 956 |
| BmeT110I | CYCGRG | 1 cut(s) | 80 |
| BmgT120I | GGNCC | 4 cut(s) | 134, 923, 956, 1102 |
| BmiI | GGNNCC | 2 cut(s) | 565, 925 |
| BmrFI | CCNGG | 3 cut(s) | 659, 717, 1106 |
| BmrI | ACTGGG | 1 cut(s) | 577 |
| BmsI | GCATC | 4 cut(s) | 757, 1170, 1848, 1903 |
| BmuI | ACTGGG | 1 cut(s) | 577 |
| BpiI | GAAGAC | 3 cut(s) | 234, 1034, 1198 |
| BpmI | CTGGAG | 1 cut(s) | 959 |
| Bpu14I | TTCGAA | 1 cut(s) | 1254 |
| BpuEI | CTTGAG | 2 cut(s) | 589, 902 |
| BpuMI | CCSGG | 1 cut(s) | 1106 |
| BsaJI | CCNNGG | 4 cut(s) | 829, 1105, 1574, 1817 |
| BsaWI | WCCGGW | 1 cut(s) | 1066 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 1140, 1580, 1909 |
| Bse1I | ACTGG | 4 cut(s) | 583, 942, 1276, 1564 |
| Bse3DI | GCAATG | 1 cut(s) | 98 |
| BseBI | CCWGG | 2 cut(s) | 659, 717 |
| BseDI | CCNNGG | 4 cut(s) | 829, 1105, 1574, 1817 |
| BseGI | GGATG | 5 cut(s) | 217, 707, 1185, 1613, 1918 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 1140, 1580, 1909 |
| BseMI | GCAATG | 1 cut(s) | 98 |
| BseMII | CTCAG | 3 cut(s) | 108, 341, 1587 |
| BseNI | ACTGG | 4 cut(s) | 583, 942, 1276, 1564 |
| BseRI | GAGGAG | 2 cut(s) | 333, 1382 |
| BseXI | GCAGC | 5 cut(s) | 353, 356, 359, 1378, 1384 |
| Bsh1236I | CGCG | 1 cut(s) | 1472 |
| BshFI | GGCC | 4 cut(s) | 90, 136, 924, 1104 |
| BshNI | GGYRCC | 1 cut(s) | 563 |
| BsiHKAI | GWGCWC | 2 cut(s) | 81, 165 |
| BsiHKCI | CYCGRG | 1 cut(s) | 80 |
| BsiSI | CCGG | 4 cut(s) | 140, 548, 1067, 1105 |
| BslFI | GGGAC | 4 cut(s) | 28, 1184, 1460, 1481 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 1140, 1580, 1909 |
| BsmAI | GTCTC | 1 cut(s) | 1308 |
| BsmFI | GGGAC | 4 cut(s) | 28, 1184, 1460, 1481 |
| BsnI | GGCC | 4 cut(s) | 90, 136, 924, 1104 |
| BsoBI | CYCGRG | 1 cut(s) | 80 |
| Bsp119I | TTCGAA | 1 cut(s) | 1254 |
| Bsp1286I | GDGCHC | 2 cut(s) | 81, 165 |
| Bsp1407I | TGTACA | 2 cut(s) | 813, 1455 |
| Bsp143I | GATC | 5 cut(s) | 112, 783, 1187, 1704, 1729 |
| Bsp19I | CCATGG | 1 cut(s) | 829 |
| BspACI | CCGC | 8 cut(s) | 137, 281, 682, 711, 1210, 1472, 1708, 1837 |
| BspANI | GGCC | 4 cut(s) | 90, 136, 924, 1104 |
| BspCNI | CTCAG | 3 cut(s) | 109, 340, 1588 |
| BspFNI | CGCG | 1 cut(s) | 1472 |
| BspLI | GGNNCC | 2 cut(s) | 565, 925 |
| BspMAI | CTGCAG | 2 cut(s) | 669, 1396 |
| BspMI | ACCTGC | 1 cut(s) | 533 |
| BspPI | GGATC | 4 cut(s) | 107, 791, 1699, 1724 |
| BspQI | GCTCTTC | 2 cut(s) | 431, 749 |
| BspT104I | TTCGAA | 1 cut(s) | 1254 |
| BspT107I | GGYRCC | 1 cut(s) | 563 |
| BspTI | CTTAAG | 2 cut(s) | 557, 1907 |
| BsrBI | CCGCTC | 1 cut(s) | 1710 |
| BsrDI | GCAATG | 1 cut(s) | 98 |
| BsrGI | TGTACA | 2 cut(s) | 813, 1455 |
| BsrI | ACTGG | 4 cut(s) | 583, 942, 1276, 1564 |
| BssECI | CCNNGG | 4 cut(s) | 829, 1105, 1574, 1817 |
| BssMI | GATC | 5 cut(s) | 112, 783, 1187, 1704, 1729 |
| BssT1I | CCWWGG | 2 cut(s) | 829, 1574 |
| Bst2UI | CCWGG | 2 cut(s) | 659, 717 |
| Bst4CI | ACNGT | 2 cut(s) | 233, 1949 |
| Bst6I | CTCTTC | 5 cut(s) | 171, 246, 431, 749, 1865 |
| BstAFI | CTTAAG | 2 cut(s) | 557, 1907 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1400 |
| BstAUI | TGTACA | 2 cut(s) | 813, 1455 |
| BstBI | TTCGAA | 1 cut(s) | 1254 |
| BstC8I | GCNNGC | 3 cut(s) | 7, 402, 1571 |
| BstDEI | CTNAG | 4 cut(s) | 117, 327, 333, 1596 |
| BstDSI | CCRYGG | 2 cut(s) | 829, 1817 |
| BstEII | GGTNACC | 1 cut(s) | 719 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1907 |
| BstF5I | GGATG | 5 cut(s) | 217, 707, 1185, 1613, 1918 |
| BstFNI | CGCG | 1 cut(s) | 1472 |
| BstHHI | GCGC | 2 cut(s) | 406, 1918 |
| BstKTI | GATC | 5 cut(s) | 115, 786, 1190, 1707, 1732 |
| BstMAI | GTCTC | 1 cut(s) | 1308 |
| BstMBI | GATC | 5 cut(s) | 112, 783, 1187, 1704, 1729 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 347, 1400, 1950 |
| BstNI | CCWGG | 2 cut(s) | 659, 717 |
| BstNSI | RCATGY | 2 cut(s) | 424, 1216 |
| BstPI | GGTNACC | 1 cut(s) | 719 |
| BstSCI | CCNGG | 3 cut(s) | 657, 715, 1104 |
| BstSFI | CTRYAG | 3 cut(s) | 639, 665, 1392 |
| BstUI | CGCG | 1 cut(s) | 1472 |
| BstV1I | GCAGC | 5 cut(s) | 353, 356, 359, 1378, 1384 |
| BstV2I | GAAGAC | 3 cut(s) | 234, 1034, 1198 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1314 |
| BsuI | GTATCC | 1 cut(s) | 1431 |
| BsuRI | GGCC | 4 cut(s) | 90, 136, 924, 1104 |
| BtgI | CCRYGG | 2 cut(s) | 829, 1817 |
| BtgZI | GCGATG | 2 cut(s) | 365, 632 |
| BtsCI | GGATG | 5 cut(s) | 217, 707, 1185, 1613, 1918 |
| BtsIMutI | CAGTG | 6 cut(s) | 238, 704, 1227, 1571, 1943, 1945 |
| BveI | ACCTGC | 1 cut(s) | 533 |
| Cac8I | GCNNGC | 3 cut(s) | 7, 402, 1571 |
| CaiI | CAGNNNCTG | 1 cut(s) | 664 |
| CfoI | GCGC | 2 cut(s) | 406, 1918 |
| Cfr13I | GGNCC | 4 cut(s) | 134, 923, 956, 1102 |
| CseI | GACGC | 2 cut(s) | 1283, 1478 |
| CsiI | ACCWGGT | 2 cut(s) | 657, 715 |
| Csp6I | GTAC | 8 cut(s) | 154, 438, 632, 814, 878, 1279, 1456, 1789 |
| CviAII | CATG | 8 cut(s) | 421, 430, 485, 830, 1038, 1097, 1213, 1516 |
| CviQI | GTAC | 8 cut(s) | 154, 438, 632, 814, 878, 1279, 1456, 1789 |
| DdeI | CTNAG | 4 cut(s) | 117, 327, 333, 1596 |
| DpnI | GATC | 5 cut(s) | 114, 785, 1189, 1706, 1731 |
| DpnII | GATC | 5 cut(s) | 112, 783, 1187, 1704, 1729 |
| DraI | TTTAAA | 1 cut(s) | 1810 |
| Eam1104I | CTCTTC | 5 cut(s) | 171, 246, 431, 749, 1865 |
| EarI | CTCTTC | 5 cut(s) | 171, 246, 431, 749, 1865 |
| Ecl136II | GAGCTC | 1 cut(s) | 79 |
| Eco130I | CCWWGG | 2 cut(s) | 829, 1574 |
| Eco24I | GRGCYC | 1 cut(s) | 81 |
| Eco32I | GATATC | 1 cut(s) | 31 |
| Eco47I | GGWCC | 1 cut(s) | 956 |
| Eco53kI | GAGCTC | 1 cut(s) | 79 |
| Eco57I | CTGAAG | 4 cut(s) | 195, 323, 1868, 1889 |
| Eco88I | CYCGRG | 1 cut(s) | 80 |
| Eco91I | GGTNACC | 1 cut(s) | 719 |
| EcoICRI | GAGCTC | 1 cut(s) | 79 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1907 |
| EcoO109I | RGGNCCY | 2 cut(s) | 923, 956 |
| EcoO65I | GGTNACC | 1 cut(s) | 719 |
| EcoRII | CCWGG | 2 cut(s) | 657, 715 |
| EcoRV | GATATC | 1 cut(s) | 31 |
| EcoT14I | CCWWGG | 2 cut(s) | 829, 1574 |
| EcoT22I | ATGCAT | 1 cut(s) | 505 |
| EcoT38I | GRGCYC | 1 cut(s) | 81 |
| ErhI | CCWWGG | 2 cut(s) | 829, 1574 |
| FaeI | CATG | 8 cut(s) | 424, 433, 488, 833, 1041, 1100, 1216, 1519 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 551, 583, 743, 775 |
| FaqI | GGGAC | 4 cut(s) | 28, 1184, 1460, 1481 |
| FatI | CATG | 8 cut(s) | 420, 429, 484, 829, 1037, 1096, 1212, 1515 |
| FblI | GTMKAC | 1 cut(s) | 1272 |
| Fnu4HI | GCNGC | 8 cut(s) | 10, 137, 342, 345, 348, 682, 1373, 1392 |
| FokI | GGATG | 5 cut(s) | 224, 694, 1192, 1600, 1925 |
| FriOI | GRGCYC | 1 cut(s) | 81 |
| Fsp4HI | GCNGC | 8 cut(s) | 10, 137, 342, 345, 348, 682, 1373, 1392 |
| FspBI | CTAG | 5 cut(s) | 413, 602, 848, 989, 1692 |
| FspI | TGCGCA | 1 cut(s) | 405 |
| GlaI | GCGC | 2 cut(s) | 405, 1917 |
| GluI | GCNGC | 8 cut(s) | 10, 137, 342, 345, 348, 682, 1373, 1392 |
| GsuI | CTGGAG | 1 cut(s) | 959 |
| HaeIII | GGCC | 4 cut(s) | 90, 136, 924, 1104 |
| HapII | CCGG | 4 cut(s) | 140, 548, 1067, 1105 |
| HgaI | GACGC | 2 cut(s) | 1283, 1478 |
| HhaI | GCGC | 2 cut(s) | 406, 1918 |
| Hin1II | CATG | 8 cut(s) | 424, 433, 488, 833, 1041, 1100, 1216, 1519 |
| Hin6I | GCGC | 2 cut(s) | 404, 1916 |
| HinP1I | GCGC | 2 cut(s) | 404, 1916 |
| HincII | GTYRAC | 1 cut(s) | 1009 |
| HindII | GTYRAC | 1 cut(s) | 1009 |
| HindIII | AAGCTT | 1 cut(s) | 370 |
| HinfI | GANTC | 9 cut(s) | 323, 644, 674, 866, 1232, 1359, 1636, 1655, 1802 |
| HpaI | GTTAAC | 1 cut(s) | 1009 |
| HpaII | CCGG | 4 cut(s) | 140, 548, 1067, 1105 |
| HphI | GGTGA | 3 cut(s) | 308, 588, 713 |
| Hpy166II | GTNNAC | 7 cut(s) | 109, 299, 836, 1009, 1029, 1273, 1456 |
| Hpy188I | TCNGA | 8 cut(s) | 25, 330, 379, 649, 1555, 1714, 1848, 1869 |
| Hpy8I | GTNNAC | 7 cut(s) | 109, 299, 836, 1009, 1029, 1273, 1456 |
| Hpy99I | CGWCG | 2 cut(s) | 161, 209 |
| HpyCH4III | ACNGT | 2 cut(s) | 233, 1949 |
| HpyCH4IV | ACGT | 2 cut(s) | 156, 1434 |
| HpyCH4V | TGCA | 9 cut(s) | 12, 98, 503, 667, 1117, 1183, 1394, 1403, 1736 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 347, 1400, 1950 |
| HpyF3I | CTNAG | 4 cut(s) | 117, 327, 333, 1596 |
| HpySE526I | ACGT | 2 cut(s) | 156, 1434 |
| Hsp92II | CATG | 8 cut(s) | 424, 433, 488, 833, 1041, 1100, 1216, 1519 |
| HspAI | GCGC | 2 cut(s) | 404, 1916 |
| KspAI | GTTAAC | 1 cut(s) | 1009 |
| Kzo9I | GATC | 5 cut(s) | 112, 783, 1187, 1704, 1729 |
| LguI | GCTCTTC | 2 cut(s) | 431, 749 |
| LmnI | GCTCC | 2 cut(s) | 622, 1369 |
| Lsp1109I | GCAGC | 5 cut(s) | 353, 356, 359, 1378, 1384 |
| LweI | GCATC | 4 cut(s) | 757, 1170, 1848, 1903 |
| MabI | ACCWGGT | 2 cut(s) | 657, 715 |
| MaeI | CTAG | 5 cut(s) | 413, 602, 848, 989, 1692 |
| MaeII | ACGT | 2 cut(s) | 156, 1434 |
| MaeIII | GTNAC | 4 cut(s) | 470, 719, 1287, 1584 |
| MalI | GATC | 5 cut(s) | 114, 785, 1189, 1706, 1731 |
| MbiI | CCGCTC | 1 cut(s) | 1710 |
| MboI | GATC | 5 cut(s) | 112, 783, 1187, 1704, 1729 |
| MhlI | GDGCHC | 2 cut(s) | 81, 165 |
| MlyI | GAGTC | 2 cut(s) | 1226, 1649 |
| MmeI | TCCRAC | 3 cut(s) | 340, 402, 1533 |
| Mph1103I | ATGCAT | 1 cut(s) | 505 |
| MroXI | GAANNNNTTC | 3 cut(s) | 577, 693, 1682 |
| MseI | TTAA | 9 cut(s) | 71, 558, 654, 729, 1008, 1284, 1548, 1809, 1908 |
| MslI | CAYNNNNRTG | 2 cut(s) | 1036, 1312 |
| MspCI | CTTAAG | 2 cut(s) | 557, 1907 |
| MspI | CCGG | 4 cut(s) | 140, 548, 1067, 1105 |
| MspR9I | CCNGG | 3 cut(s) | 659, 717, 1106 |
| MvaI | CCWGG | 2 cut(s) | 659, 717 |
| MvnI | CGCG | 1 cut(s) | 1472 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 347, 1400, 1950 |
| NciI | CCSGG | 1 cut(s) | 1106 |
| NcoI | CCATGG | 1 cut(s) | 829 |
| NdeII | GATC | 5 cut(s) | 112, 783, 1187, 1704, 1729 |
| NlaIII | CATG | 8 cut(s) | 424, 433, 488, 833, 1041, 1100, 1216, 1519 |
| NlaIV | GGNNCC | 2 cut(s) | 565, 925 |
| NmuCI | GTSAC | 2 cut(s) | 719, 1584 |
| NsbI | TGCGCA | 1 cut(s) | 405 |
| NsiI | ATGCAT | 1 cut(s) | 505 |
| NspI | RCATGY | 2 cut(s) | 424, 1216 |
| NspV | TTCGAA | 1 cut(s) | 1254 |
| PaeR7I | CTCGAG | 1 cut(s) | 80 |
| PciSI | GCTCTTC | 2 cut(s) | 431, 749 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 165 |
| PdmI | GAANNNNTTC | 3 cut(s) | 577, 693, 1682 |
| PfeI | GAWTC | 7 cut(s) | 323, 644, 674, 866, 1359, 1636, 1802 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1140 |
| PkrI | GCNGC | 8 cut(s) | 11, 138, 343, 346, 349, 683, 1374, 1393 |
| PleI | GAGTC | 2 cut(s) | 1226, 1649 |
| PpsI | GAGTC | 2 cut(s) | 1226, 1649 |
| PpuMI | RGGWCCY | 1 cut(s) | 956 |
| Psp124BI | GAGCTC | 1 cut(s) | 81 |
| Psp1406I | AACGTT | 1 cut(s) | 1434 |
| Psp5II | RGGWCCY | 1 cut(s) | 956 |
| Psp6I | CCWGG | 2 cut(s) | 657, 715 |
| PspEI | GGTNACC | 1 cut(s) | 719 |
| PspGI | CCWGG | 2 cut(s) | 657, 715 |
| PspN4I | GGNNCC | 2 cut(s) | 565, 925 |
| PspPI | GGNCC | 4 cut(s) | 134, 923, 956, 1102 |
| PspPPI | RGGWCCY | 1 cut(s) | 956 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 137, 169 |
| PstI | CTGCAG | 2 cut(s) | 669, 1396 |
| PstNI | CAGNNNCTG | 1 cut(s) | 664 |
| RsaI | GTAC | 8 cut(s) | 155, 439, 633, 815, 879, 1280, 1457, 1790 |
| RsaNI | GTAC | 8 cut(s) | 154, 438, 632, 814, 878, 1279, 1456, 1789 |
| RseI | CAYNNNNRTG | 2 cut(s) | 1036, 1312 |
| SacI | GAGCTC | 1 cut(s) | 81 |
| SapI | GCTCTTC | 2 cut(s) | 431, 749 |
| SaqAI | TTAA | 9 cut(s) | 71, 558, 654, 729, 1008, 1284, 1548, 1809, 1908 |
| SatI | GCNGC | 8 cut(s) | 10, 137, 342, 345, 348, 682, 1373, 1392 |
| Sau3AI | GATC | 5 cut(s) | 112, 783, 1187, 1704, 1729 |
| Sau96I | GGNCC | 4 cut(s) | 134, 923, 956, 1102 |
| SchI | GAGTC | 2 cut(s) | 1226, 1649 |
| ScrFI | CCNGG | 3 cut(s) | 659, 717, 1106 |
| SduI | GDGCHC | 2 cut(s) | 81, 165 |
| SexAI | ACCWGGT | 2 cut(s) | 657, 715 |
| SfaNI | GCATC | 4 cut(s) | 757, 1170, 1848, 1903 |
| SfcI | CTRYAG | 3 cut(s) | 639, 665, 1392 |
| Sfr274I | CTCGAG | 1 cut(s) | 80 |
| SfuI | TTCGAA | 1 cut(s) | 1254 |
| SinI | GGWCC | 1 cut(s) | 956 |
| SlaI | CTCGAG | 1 cut(s) | 80 |
| SmiI | ATTTAAAT | 1 cut(s) | 1810 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 1036, 1312 |
| SmlI | CTYRAG | 5 cut(s) | 80, 557, 568, 881, 1907 |
| SmoI | CTYRAG | 5 cut(s) | 80, 557, 568, 881, 1907 |
| SsiI | CCGC | 8 cut(s) | 137, 281, 682, 711, 1210, 1472, 1708, 1837 |
| SspMI | CTAG | 5 cut(s) | 413, 602, 848, 989, 1692 |
| SstI | GAGCTC | 1 cut(s) | 81 |
| StyD4I | CCNGG | 3 cut(s) | 657, 715, 1104 |
| StyI | CCWWGG | 2 cut(s) | 829, 1574 |
| SwaI | ATTTAAAT | 1 cut(s) | 1810 |
| TaaI | ACNGT | 2 cut(s) | 233, 1949 |
| TaiI | ACGT | 2 cut(s) | 159, 1437 |
| TaqI | TCGA | 6 cut(s) | 75, 81, 159, 1254, 1703, 1797 |
| TatI | WGTACW | 5 cut(s) | 437, 813, 877, 1278, 1455 |
| TauI | GCSGC | 2 cut(s) | 139, 684 |
| TfiI | GAWTC | 7 cut(s) | 323, 644, 674, 866, 1359, 1636, 1802 |
| Tru1I | TTAA | 9 cut(s) | 71, 558, 654, 729, 1008, 1284, 1548, 1809, 1908 |
| Tru9I | TTAA | 9 cut(s) | 71, 558, 654, 729, 1008, 1284, 1548, 1809, 1908 |
| TscAI | CASTG | 6 cut(s) | 238, 704, 1234, 1571, 1943, 1952 |
| TseFI | GTSAC | 2 cut(s) | 719, 1584 |
| TseI | GCWGC | 6 cut(s) | 9, 341, 344, 347, 1372, 1391 |
| Tsp45I | GTSAC | 2 cut(s) | 719, 1584 |
| TspRI | CASTG | 6 cut(s) | 238, 704, 1234, 1571, 1943, 1952 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1140 |
| Vha464I | CTTAAG | 2 cut(s) | 557, 1907 |
| VpaK11BI | GGWCC | 1 cut(s) | 956 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1907 |
| XapI | RAATTY | 4 cut(s) | 650, 693, 1408, 1811 |
| XbaI | TCTAGA | 1 cut(s) | 412 |
| XceI | RCATGY | 2 cut(s) | 424, 1216 |
| XhoI | CTCGAG | 1 cut(s) | 80 |
| XmiI | GTMKAC | 1 cut(s) | 1272 |
| XmnI | GAANNNNTTC | 3 cut(s) | 577, 693, 1682 |
| XspI | CTAG | 5 cut(s) | 413, 602, 848, 989, 1692 |
| Zsp2I | ATGCAT | 1 cut(s) | 505 |