Rw5G044290

Belongs to the peptidase M16 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000891
Physical Location & Seq
Reverse (-)
77893738 .. 77901914
8177 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G044290.1

Sequence Viewer

Length: 3084 bp
ATGGCTCGCCGGACTTTCTCATCGGACGACGTCGTTTTGAAGTCTCCGAATGATAGACGACTGTACCGATTAATCGAGCTCGAGAATGGCCTCACTGCTTTGCTAGTCCACGATCCTGAGATTGGAGATTCCCAACTCTCCAATGGCTCTGAGGAAGCTGAGATGGAAGAAGAAGAAGAAGGAATGGAGGAGGATGAGGATGAGGAAGACGACGATGAAGAAGACGAAGATAGTGAAGGAGAGGATGAAGAGATGGATGAAGATGATGAGGGAGAAGATGAAGATGGACTGAAGAAGAAAAAGGGAGGGGATTCTCAGACTAAGAAGGCAGCAGCGGCAATGTGTGTAGGAATAGGCAGCTTCTCTGACCCTCCTGAGGCACCGGGGCTTGCACACTTTCTAGAACACATGCTCTTTATGGGGAGTACAGAATTTCCAGATGAAAATGAGTATGACAGTTACTTGTCCAAGCATGGAGGGTGTTCAAATGCATACACGGAGGTAGAGCATACTTGCTACCATTTTGAAGTGAAACGAGAGTTTCTCAAGGGTGCCTTGACCAGATTTTCTCAGTTCTTTGTTTCACCCCTTGTAAAAATTGAAGCCATGGAGCGGGAGGTACAGGCTGTAGATTCAGAGTTTAACCAGGTTCAGCAGAACGATGCTTGCCGCCTTGAACAACTTCAATGCCATACAGCCTCACCTGGTCACCCATTTAATAGATTCGCATGGGGGAATAAAAAAAGCTTGAGCAATGCCATGGAAAAAGGGATCAACTTGCGGGAACAAATATTGAAATTGTACAGAGATTATTACCATGGTGGATTGATGAAGCTAGTTGTCATAGGAGGAGAATCTCTTGATGTACTTGAGAATTGGGTTTTGGAATTGTTTGGTGACGTCAAAAAAGGTCCCCAAGTAAATCTGGAATTCAAGGCGGAAGGTCCCATTTGGAAAGCTGGAAAAGTTTACAGGCTAGAGGCTGTTAAAGATGTTCATATACTCCACTTAACATGGACATTGCCATGCCTTCGTCAAGACTATTTGAAGAAATCAGAAGATTATTTATCTCATCTCCTTGGGCATGAGGGCAGGGGAAGTTTGCATTCCCATTTCAAAGTTAAGGGGTGGGCAACATCTCTGGCTGCTGGTGTTGGGGATGACGGGATGCATCGTTCTTCTGTGTCTTATGTCTTTCGCATGGACATTCATCTCACTGACTCTGGATTGGATAAGATTTTTGATATAATTGGCTTGGTCTACCAATACATAAAGTTATTGCATCAAATGTCCCCACAAGAATGGATATTTAAGGAACTGCAGGATACTGGGAACATGGAATTTAGATTTGCAGAGGAGCAGCCTCAGGATGATTATGCCTCAGAACTTGCAGGAAATTTACTAATTTATGCAGCAGAACATGTTATTTATGGGTCCTACGCATACAAGATTTGGGATGAGGAATCGATCAAATATGTTCTTGATTTCCTTAGACCAGAAAACATGAGGATTGATGTTGTATCAAAGCCCTCATTTAAGTCAGAAGATTTCCAGTGCGAACCGTGGTTTGGGTCACATTATACTGAGGAAGATATATCTCCATCTTTGATAGATTTGTGGAAGGACCCTCCAGAAATTGATGTTTCATTGCATCTCCCAGAAAAGAATGAGTTCATTCCTACTGATTTTTCCATTCGTTCTGAAGATCTTGATACTACAGATACAGCTTTTCCGAGATGTATACTGGATGAGCCATTGGTGAAGTTCTGGTACAAACTTGATAATACTTTTAAACTTCCGCGGGCAAATACTTATTTCCGCGTCAATCCCAAGGGTGGATATGATAATGTGAAGAGTTGTGTCTTGACTGAACTATATGTATCCCTCCTCAAAGATGAGCTGAATGAGATTGTTTATCAGGCTAGTGTTGCCAAGCTGGAAACTTCGGTATCTGTATCGACTGACAAGCTGGAGCTAAAGGTCTACGGTTTCAATGACAAGCTTCCAGCTCTGTTGTCAAAGATTCTGAAAACAACCAAAAGTTTCATGCCAACTTCTGATCGTTTTATGGTTATCAAAGAAGATATGGAGCGAAAGTTAAAGAACACCAATATGAAGCCTCTGAGTCACTCTACATACTTGAGAGTGCAAGTTTTGTTCCAGAGATTCTATGATGTTGATGAGAAGTTGCATGTTTTAAGTGGACTGTCTATTTCTGATATGAAGTCATTTATTCCCCAACTTTGGTCCCAGGTATACATTGAAGGCCTCTGTCATGGAAATTTGTCAGAAAAAGAAGCAATCAGCCTTTCAGATATATTTAAAACAAATTTTTGTGTACAACCACTTCCAATTGAACTGAGGCGCAGTGAACAGTGTATTTGTCTTCCTCCTTCTGCTAACCTCATTAGAGATGTCAGTGTGAAGAATCAGTCTGAAAAAAATTCAGTGATTGAGCTGTATTTTCAAATTGAGCGGGAATTGAGGATCGAGTCCCCCAGATTGAGAGCCCTGATAGATCTTTTCAATGAAATTGTGGAGGAACCACTTTTCAATCAGCTAAGGACGAAGGAGCAGCTTGGTTATACTGTTCATTGTGGCCCACGGGTTACTTGCAATATATTTGGATTTTATTTTTGCGTTCAGTCAGCTGAGTACAACCCAATCTACTTGCAAGGGAGAGTTGAGAACTTTATAAATAGTCTGGAGGAGTTGTTGCAAGGACTGGATGATGACTCTTTTGAGGATTACAGAGCTGGGCTAATTGCAAAGCTGTTGGAGAAAGATCCATCCCTCCATTATGAAACCAATCGATATTGGTTACAGATTACCGATAAAATGTATATGTTTGACTATACAAAACAGGAGGCAGAACGACTCAAGAGCATTCAGAAGGAGGATGTCATCAACTGGTACAAAACATATTTGCAACAATCATCTCCCAAGTGTCGGAGACTTGCAATTCGTGTTTGGGGATGCAACACAGACTTGAAAGAGGCTGAAGCACGGCCGGAGTCTGTAAAAGTCATTGAAGACCTTGCAGCCTTTAAGATGTCATCTGAGTTCTATCCAAGCATTTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0003674 GO:0003824 GO:0004175 GO:0004222 GO:0005102 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005777 GO:0005782 GO:0005829 GO:0006508 GO:0006518 GO:0006605 GO:0006625 GO:0006807 GO:0006810 GO:0006886 GO:0006996 GO:0007031 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007169 GO:0007275 GO:0007568 GO:0008104 GO:0008144 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0008270 GO:0008286 GO:0008340 GO:0009056 GO:0009057 GO:0009719 GO:0009725 GO:0009893 GO:0009894 GO:0009896 GO:0009986 GO:0009987 GO:0010033 GO:0010243 GO:0010259 GO:0010604 GO:0010815 GO:0010992 GO:0015031 GO:0015833 GO:0016043 GO:0016787 GO:0017046 GO:0017076 GO:0017144 GO:0019222 GO:0019538 GO:0019725 GO:0022607 GO:0023052 GO:0030163 GO:0030554 GO:0031334 GO:0031907 GO:0031974 GO:0032459 GO:0032461 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0032868 GO:0032869 GO:0032870 GO:0033036 GO:0033218 GO:0033365 GO:0034613 GO:0034641 GO:0035639 GO:0036094 GO:0042176 GO:0042221 GO:0042277 GO:0042562 GO:0042579 GO:0042592 GO:0042737 GO:0042802 GO:0042803 GO:0042886 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043171 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043254 GO:0043434 GO:0043559 GO:0043574 GO:0043603 GO:0043933 GO:0044085 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044421 GO:0044422 GO:0044424 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0045732 GO:0046872 GO:0046907 GO:0046914 GO:0046983 GO:0048518 GO:0048522 GO:0048856 GO:0050435 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051259 GO:0051260 GO:0051603 GO:0051641 GO:0051649 GO:0051716 GO:0060255 GO:0065003 GO:0065007 GO:0065008 GO:0070011 GO:0070013 GO:0070727 GO:0070887 GO:0071310 GO:0071375 GO:0071417 GO:0071495 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072662 GO:0072663 GO:0080090 GO:0097159 GO:0097242 GO:0097367 GO:0140030 GO:0140035 GO:0140036 GO:0140096 GO:1901142 GO:1901143 GO:1901265 GO:1901363 GO:1901564 GO:1901565 GO:1901575 GO:1901652 GO:1901653 GO:1901698 GO:1901699 GO:1901700 GO:1901701
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_M16 Peptidase_M16_C Peptidase_M16_M

Protein Analysis

1027

Amino Acids

118.48

Weight (kDa)

4.82

Isoelectric Point (pI)

46.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M16 PF00675 105 - 228 1.1e-32 Insulinase (Peptidase family M16)
Peptidase_M16_C PF05193 261 - 441 8.4e-19 Peptidase M16 inactive domain
Peptidase_M16_M PF16187 448 - 731 1.1e-90 Middle or third domain of peptidase_M16
Peptidase_M16_C PF05193 736 - 921 6.2e-17 Peptidase M16 inactive domain
PqqF-like_C_4 PF22456 841 - 940 3.4e-25 PQQ synthase PqqF-like, C-terminal lobe domain 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2696
AatII GACGTC 2 cut(s) 33, 903
AccB1I GGYRCC 2 cut(s) 379, 551
AccBSI CCGCTC 2 cut(s) 613, 2476
AccI GTMKAC 4 cut(s) 1260, 1741, 1983, 2256
AccII CGCG 2 cut(s) 1801, 1821
AciI CCGC 9 cut(s) 335, 613, 670, 781, 938, 1799, 1801, 1819, 2476
AclWI GGATC 4 cut(s) 107, 779, 2495, 2780
AcoI YGGCCR 1 cut(s) 3008
AcsI RAATTY 7 cut(s) 431, 929, 1340, 1396, 2281, 2329, 2443
AcuI CTGAAG 3 cut(s) 311, 1722, 3021
AcyI GRCGYC 2 cut(s) 30, 900
AfaI GTAC 9 cut(s) 65, 427, 621, 803, 867, 1772, 2340, 2657, 2915
AfiI CCNNNNNNNGG 7 cut(s) 122, 376, 612, 1568, 1835, 2244, 2949
AflIII ACRYGT 1 cut(s) 1420
AjnI CCWGG 3 cut(s) 645, 703, 2250
AjuI GAANNNNNNNTTGG 4 cut(s) 866, 898, 1551, 1583
AloI GAACNNNNNNTCC 2 cut(s) 1159, 1191
Alw21I GWGCWC 1 cut(s) 81
Alw26I GTCTC 2 cut(s) 48, 2947
AlwI GGATC 4 cut(s) 107, 779, 2495, 2780
Ama87I CYCGRG 1 cut(s) 80
AoxI GGCC 4 cut(s) 88, 2266, 2599, 3008
ApeKI GCWGC 8 cut(s) 329, 332, 357, 1145, 1360, 1412, 2575, 3041
ApoI RAATTY 7 cut(s) 431, 929, 1340, 1396, 2281, 2329, 2443
ArsI GACNNNNNNTTYG 2 cut(s) 2946, 2978
AseI ATTAAT 1 cut(s) 71
Asp700I GAANNNNTTC 2 cut(s) 681, 1670
AspLEI GCGC 1 cut(s) 2367
AspS9I GGNCC 6 cut(s) 911, 944, 1434, 1624, 2247, 2600
AsuC2I CCSGG 1 cut(s) 384
AsuHPI GGTGA 5 cut(s) 576, 693, 701, 908, 1771
AvaI CYCGRG 1 cut(s) 80
AvaII GGWCC 5 cut(s) 911, 944, 1434, 1624, 2247
AxyI CCTNAGG 2 cut(s) 375, 1365
BaeI ACNNNNGTAYC 4 cut(s) 47, 80, 1932, 1965
BanI GGYRCC 2 cut(s) 379, 551
BanII GRGCYC 2 cut(s) 81, 2512
BarI GAAGNNNNNNTAC 4 cut(s) 1754, 1786, 2331, 2363
BbsI GAAGAC 4 cut(s) 213, 228, 2378, 3039
Bbv12I GWGCWC 1 cut(s) 81
BbvI GCAGC 8 cut(s) 341, 344, 369, 1132, 1372, 1424, 2587, 3053
BccI CCATC 5 cut(s) 157, 247, 278, 1609, 2797
BceAI ACGGC 1 cut(s) 3023
BciT130I CCWGG 3 cut(s) 647, 705, 2252
BciVI GTATCC 2 cut(s) 1318, 1891
BcnI CCSGG 1 cut(s) 384
BcoDI GTCTC 2 cut(s) 48, 2947
BfaI CTAG 5 cut(s) 104, 401, 836, 977, 1923
BfmI CTRYAG 3 cut(s) 627, 1319, 1716
BfuI GTATCC 2 cut(s) 1318, 1891
BglII AGATCT 2 cut(s) 1705, 2518
Bme1390I CCNGG 4 cut(s) 384, 647, 705, 2252
Bme18I GGWCC 5 cut(s) 911, 944, 1434, 1624, 2247
BmeT110I CYCGRG 1 cut(s) 80
BmgT120I GGNCC 6 cut(s) 911, 944, 1434, 1624, 2247, 2600
BmiI GGNNCC 8 cut(s) 381, 553, 913, 946, 1435, 1626, 2249, 2544
BmrFI CCNGG 4 cut(s) 384, 647, 705, 2252
BmrI ACTGGG 1 cut(s) 1338
BmsI GCATC 6 cut(s) 652, 1158, 1180, 1291, 1660, 2966
BmuI ACTGGG 1 cut(s) 1338
BpiI GAAGAC 4 cut(s) 213, 228, 2378, 3039
BplI GAGNNNNNCTC 2 cut(s) 528, 560
BpmI CTGGAG 3 cut(s) 1614, 1991, 2726
Bpu10I CCTNAGC 1 cut(s) 2561
BpuEI CTTGAG 5 cut(s) 530, 769, 890, 2161, 2864
BpuMI CCSGG 1 cut(s) 384
Bsa29I ATCGAT 2 cut(s) 1466, 2812
BsaHI GRCGYC 2 cut(s) 30, 900
BsaXI ACNNNNNCTCC 4 cut(s) 2081, 2111, 2699, 2729
Bsc4I CCNNNNNNNGG 7 cut(s) 122, 376, 612, 1568, 1835, 2244, 2949
Bse1I ACTGG 5 cut(s) 1333, 1552, 1749, 2730, 2915
Bse21I CCTNAGG 2 cut(s) 375, 1365
Bse3DI GCAATG 4 cut(s) 345, 760, 1019, 1646
BseBI CCWGG 3 cut(s) 647, 705, 2252
BseCI ATCGAT 2 cut(s) 1466, 2812
BseLI CCNNNNNNNGG 7 cut(s) 122, 376, 612, 1568, 1835, 2244, 2949
BseMI GCAATG 4 cut(s) 345, 760, 1019, 1646
BseNI ACTGG 5 cut(s) 1333, 1552, 1749, 2730, 2915
BseRI GAGGAG 5 cut(s) 203, 864, 1370, 1877, 2723
BseX3I CGGCCG 1 cut(s) 3008
BseXI GCAGC 8 cut(s) 341, 344, 369, 1132, 1372, 1424, 2587, 3053
BseYI CCCAGC 1 cut(s) 2756
Bsh1236I CGCG 2 cut(s) 1801, 1821
Bsh1285I CGRYCG 1 cut(s) 3011
BshFI GGCC 4 cut(s) 90, 2268, 2601, 3010
BshNI GGYRCC 2 cut(s) 379, 551
BshVI ATCGAT 2 cut(s) 1466, 2812
BsiEI CGRYCG 1 cut(s) 3011
BsiHKAI GWGCWC 1 cut(s) 81
BsiHKCI CYCGRG 1 cut(s) 80
BsiSI CCGG 3 cut(s) 10, 383, 3011
BslFI GGGAC 5 cut(s) 897, 930, 1276, 2233, 2479
BslI CCNNNNNNNGG 7 cut(s) 122, 376, 612, 1568, 1835, 2244, 2949
BsmAI GTCTC 2 cut(s) 48, 2947
BsmFI GGGAC 5 cut(s) 897, 930, 1276, 2233, 2479
BsmI GAATGC 2 cut(s) 1105, 2886
BsnI GGCC 4 cut(s) 90, 2268, 2601, 3010
BsoBI CYCGRG 1 cut(s) 80
Bsp1286I GDGCHC 2 cut(s) 81, 2512
Bsp1407I TGTACA 2 cut(s) 801, 2338
Bsp143I GATC 8 cut(s) 112, 771, 1467, 1705, 2059, 2487, 2518, 2785
Bsp19I CCATGG 3 cut(s) 606, 759, 817
BspACI CCGC 9 cut(s) 335, 613, 670, 781, 938, 1799, 1801, 1819, 2476
BspANI GGCC 4 cut(s) 90, 2268, 2601, 3010
BspDI ATCGAT 2 cut(s) 1466, 2812
BspFNI CGCG 2 cut(s) 1801, 1821
BspLI GGNNCC 8 cut(s) 381, 553, 913, 946, 1435, 1626, 2249, 2544
BspMAI CTGCAG 1 cut(s) 1323
BspPI GGATC 4 cut(s) 107, 779, 2495, 2780
BspT107I GGYRCC 2 cut(s) 379, 551
BsrBI CCGCTC 2 cut(s) 613, 2476
BsrDI GCAATG 4 cut(s) 345, 760, 1019, 1646
BsrGI TGTACA 2 cut(s) 801, 2338
BsrI ACTGG 5 cut(s) 1333, 1552, 1749, 2730, 2915
BssMI GATC 8 cut(s) 112, 771, 1467, 1705, 2059, 2487, 2518, 2785
BssNAI GTATAC 2 cut(s) 1742, 2257
BssNI GRCGYC 2 cut(s) 30, 900
BssT1I CCWWGG 5 cut(s) 606, 759, 817, 1078, 1830
Bst1107I GTATAC 2 cut(s) 1742, 2257
Bst2UI CCWGG 3 cut(s) 647, 705, 2252
Bst4CI ACNGT 7 cut(s) 63, 458, 1563, 1988, 2208, 2376, 2590
Bst6I CTCTTC 2 cut(s) 243, 1847
BstACI GRCGYC 2 cut(s) 30, 900
BstAUI TGTACA 2 cut(s) 801, 2338
BstC8I GCNNGC 4 cut(s) 7, 390, 667, 1803
BstDSI CCRYGG 6 cut(s) 606, 759, 817, 1562, 1799, 2603
BstEII GGTNACC 1 cut(s) 707
BstFNI CGCG 2 cut(s) 1801, 1821
BstHHI GCGC 1 cut(s) 2367
BstKTI GATC 8 cut(s) 115, 774, 1470, 1708, 2062, 2490, 2521, 2788
BstMAI GTCTC 2 cut(s) 48, 2947
BstMBI GATC 8 cut(s) 112, 771, 1467, 1705, 2059, 2487, 2518, 2785
BstMCI CGRYCG 1 cut(s) 3011
BstMWI GCNNNNNNNGC 2 cut(s) 335, 1928
BstNI CCWGG 3 cut(s) 647, 705, 2252
BstNSI RCATGY 3 cut(s) 412, 1424, 2195
BstPI GGTNACC 1 cut(s) 707
BstSCI CCNGG 4 cut(s) 382, 645, 703, 2250
BstSFI CTRYAG 3 cut(s) 627, 1319, 1716
BstUI CGCG 2 cut(s) 1801, 1821
BstV1I GCAGC 8 cut(s) 341, 344, 369, 1132, 1372, 1424, 2587, 3053
BstV2I GAAGAC 4 cut(s) 213, 228, 2378, 3039
BstX2I RGATCY 3 cut(s) 1705, 2518, 2785
BstXI CCANNNNNNTGG 1 cut(s) 1302
BstYI RGATCY 3 cut(s) 1705, 2518, 2785
BstZ17I GTATAC 2 cut(s) 1742, 2257
BstZI CGGCCG 1 cut(s) 3008
Bsu15I ATCGAT 2 cut(s) 1466, 2812
Bsu36I CCTNAGG 2 cut(s) 375, 1365
BsuI GTATCC 2 cut(s) 1318, 1891
BsuRI GGCC 4 cut(s) 90, 2268, 2601, 3010
BsuTUI ATCGAT 2 cut(s) 1466, 2812
BtgI CCRYGG 6 cut(s) 606, 759, 817, 1562, 1799, 2603
BtsI GCAGTG 2 cut(s) 93, 2374
BtsIMutI CAGTG 7 cut(s) 93, 1215, 1559, 2374, 2381, 2425, 2454
Cac8I GCNNGC 4 cut(s) 7, 390, 667, 1803
CfoI GCGC 1 cut(s) 2367
Cfr13I GGNCC 6 cut(s) 911, 944, 1434, 1624, 2247, 2600
Cfr42I CCGCGG 1 cut(s) 1802
ClaI ATCGAT 2 cut(s) 1466, 2812
CseI GACGC 1 cut(s) 1810
CsiI ACCWGGT 2 cut(s) 645, 703
Csp6I GTAC 9 cut(s) 64, 426, 620, 802, 866, 1771, 2339, 2656, 2914
CviQI GTAC 9 cut(s) 64, 426, 620, 802, 866, 1771, 2339, 2656, 2914
DpnI GATC 8 cut(s) 114, 773, 1469, 1707, 2061, 2489, 2520, 2787
DpnII GATC 8 cut(s) 112, 771, 1467, 1705, 2059, 2487, 2518, 2785
DraI TTTAAA 2 cut(s) 1792, 2323
EaeI YGGCCR 1 cut(s) 3008
EagI CGGCCG 1 cut(s) 3008
Eam1104I CTCTTC 2 cut(s) 243, 1847
EarI CTCTTC 2 cut(s) 243, 1847
EciI GGCGGA 1 cut(s) 953
Ecl136II GAGCTC 1 cut(s) 79
EclXI CGGCCG 1 cut(s) 3008
Eco130I CCWWGG 5 cut(s) 606, 759, 817, 1078, 1830
Eco147I AGGCCT 1 cut(s) 2268
Eco24I GRGCYC 2 cut(s) 81, 2512
Eco47I GGWCC 5 cut(s) 911, 944, 1434, 1624, 2247
Eco52I CGGCCG 1 cut(s) 3008
Eco53kI GAGCTC 1 cut(s) 79
Eco57I CTGAAG 3 cut(s) 311, 1722, 3021
Eco81I CCTNAGG 2 cut(s) 375, 1365
Eco88I CYCGRG 1 cut(s) 80
Eco91I GGTNACC 1 cut(s) 707
EcoICRI GAGCTC 1 cut(s) 79
EcoO109I RGGNCCY 4 cut(s) 911, 944, 1434, 1624
EcoO65I GGTNACC 1 cut(s) 707
EcoRI GAATTC 1 cut(s) 929
EcoRII CCWGG 3 cut(s) 645, 703, 2250
EcoT14I CCWWGG 5 cut(s) 606, 759, 817, 1078, 1830
EcoT22I ATGCAT 2 cut(s) 493, 1173
EcoT38I GRGCYC 2 cut(s) 81, 2512
ErhI CCWWGG 5 cut(s) 606, 759, 817, 1078, 1830
FalI AAGNNNNNCTT 2 cut(s) 539, 571
FaqI GGGAC 5 cut(s) 897, 930, 1276, 2233, 2479
FauI CCCGC 4 cut(s) 606, 774, 1794, 2469
FblI GTMKAC 4 cut(s) 1260, 1741, 1983, 2256
FriOI GRGCYC 2 cut(s) 81, 2512
FspBI CTAG 5 cut(s) 104, 401, 836, 977, 1923
GlaI GCGC 1 cut(s) 2366
GsaI CCCAGC 1 cut(s) 2760
GsuI CTGGAG 3 cut(s) 1614, 1991, 2726
HaeIII GGCC 4 cut(s) 90, 2268, 2601, 3010
HapII CCGG 3 cut(s) 10, 383, 3011
HgaI GACGC 1 cut(s) 1810
HhaI GCGC 1 cut(s) 2367
Hin1I GRCGYC 2 cut(s) 30, 900
Hin6I GCGC 1 cut(s) 2365
HinP1I GCGC 1 cut(s) 2365
HindIII AAGCTT 2 cut(s) 745, 2000
HpaII CCGG 3 cut(s) 10, 383, 3011
HphI GGTGA 5 cut(s) 576, 693, 701, 908, 1771
Hpy166II GTNNAC 9 cut(s) 109, 970, 1261, 1742, 1984, 2204, 2257, 2339, 2372
Hpy8I GTNNAC 9 cut(s) 109, 970, 1261, 1742, 1984, 2204, 2257, 2339, 2372
Hpy99I CGWCG 3 cut(s) 32, 35, 215
HpyCH4III ACNGT 7 cut(s) 63, 458, 1563, 1988, 2208, 2376, 2590
HpyCH4IV ACGT 2 cut(s) 30, 900
HpyF10VI GCNNNNNNNGC 2 cut(s) 335, 1928
HpySE526I ACGT 2 cut(s) 30, 900
Hsp92I GRCGYC 2 cut(s) 30, 900
HspAI GCGC 1 cut(s) 2365
KspI CCGCGG 1 cut(s) 1802
Kzo9I GATC 8 cut(s) 112, 771, 1467, 1705, 2059, 2487, 2518, 2785
LmnI GCTCC 5 cut(s) 610, 1357, 1972, 2089, 2572
Lsp1109I GCAGC 8 cut(s) 341, 344, 369, 1132, 1372, 1424, 2587, 3053
LweI GCATC 6 cut(s) 652, 1158, 1180, 1291, 1660, 2966
MabI ACCWGGT 2 cut(s) 645, 703
MaeI CTAG 5 cut(s) 104, 401, 836, 977, 1923
MaeII ACGT 2 cut(s) 30, 900
MaeIII GTNAC 7 cut(s) 458, 707, 896, 1572, 2126, 2608, 2820
MalI GATC 8 cut(s) 114, 773, 1469, 1707, 2061, 2489, 2520, 2787
MbiI CCGCTC 2 cut(s) 613, 2476
MboI GATC 8 cut(s) 112, 771, 1467, 1705, 2059, 2487, 2518, 2785
MfeI CAATTG 1 cut(s) 2352
MflI RGATCY 3 cut(s) 1705, 2518, 2785
MhlI GDGCHC 2 cut(s) 81, 2512
MlyI GAGTC 6 cut(s) 1214, 2134, 2501, 2729, 2871, 3023
MmeI TCCRAC 2 cut(s) 2757, 2930
Mph1103I ATGCAT 2 cut(s) 493, 1173
MroXI GAANNNNTTC 2 cut(s) 681, 1670
MslI CAYNNNNRTG 3 cut(s) 1024, 1300, 2111
MspA1I CMGCKG 3 cut(s) 335, 1801, 2651
MspI CCGG 3 cut(s) 10, 383, 3011
MspR9I CCNGG 4 cut(s) 384, 647, 705, 2252
MunI CAATTG 1 cut(s) 2352
Mva1269I GAATGC 2 cut(s) 1105, 2886
MvaI CCWGG 3 cut(s) 647, 705, 2252
MvnI CGCG 2 cut(s) 1801, 1821
MwoI GCNNNNNNNGC 2 cut(s) 335, 1928
NciI CCSGG 1 cut(s) 384
NcoI CCATGG 3 cut(s) 606, 759, 817
NdeII GATC 8 cut(s) 112, 771, 1467, 1705, 2059, 2487, 2518, 2785
NlaIV GGNNCC 8 cut(s) 381, 553, 913, 946, 1435, 1626, 2249, 2544
NmuCI GTSAC 4 cut(s) 707, 896, 1572, 2126
NsiI ATGCAT 2 cut(s) 493, 1173
NspI RCATGY 3 cut(s) 412, 1424, 2195
PaeR7I CTCGAG 1 cut(s) 80
PceI AGGCCT 1 cut(s) 2268
PciI ACATGT 1 cut(s) 1420
PcsI WCGNNNNNNNCGW 1 cut(s) 64
PctI GAATGC 2 cut(s) 1105, 2886
PdmI GAANNNNTTC 2 cut(s) 681, 1670
PfeI GAWTC 9 cut(s) 128, 311, 632, 723, 854, 1463, 2023, 2166, 2428
PflFI GACNNNGTC 1 cut(s) 29
PleI GAGTC 6 cut(s) 1214, 2133, 2500, 2729, 2871, 3022
PpsI GAGTC 6 cut(s) 1214, 2133, 2500, 2729, 2871, 3022
PpuMI RGGWCCY 4 cut(s) 911, 944, 1434, 1624
PscI ACATGT 1 cut(s) 1420
PshBI ATTAAT 1 cut(s) 71
PsiI TTATAA 1 cut(s) 2696
Psp124BI GAGCTC 1 cut(s) 81
Psp5II RGGWCCY 4 cut(s) 911, 944, 1434, 1624
Psp6I CCWGG 3 cut(s) 645, 703, 2250
PspEI GGTNACC 1 cut(s) 707
PspFI CCCAGC 1 cut(s) 2756
PspGI CCWGG 3 cut(s) 645, 703, 2250
PspN4I GGNNCC 8 cut(s) 381, 553, 913, 946, 1435, 1626, 2249, 2544
PspPI GGNCC 6 cut(s) 911, 944, 1434, 1624, 2247, 2600
PspPPI RGGWCCY 4 cut(s) 911, 944, 1434, 1624
PstI CTGCAG 1 cut(s) 1323
PsuI RGATCY 3 cut(s) 1705, 2518, 2785
PsyI GACNNNGTC 1 cut(s) 29
PvuII CAGCTG 1 cut(s) 2651
RsaI GTAC 9 cut(s) 65, 427, 621, 803, 867, 1772, 2340, 2657, 2915
RsaNI GTAC 9 cut(s) 64, 426, 620, 802, 866, 1771, 2339, 2656, 2914
RseI CAYNNNNRTG 3 cut(s) 1024, 1300, 2111
SacI GAGCTC 1 cut(s) 81
SacII CCGCGG 1 cut(s) 1802
Sau3AI GATC 8 cut(s) 112, 771, 1467, 1705, 2059, 2487, 2518, 2785
Sau96I GGNCC 6 cut(s) 911, 944, 1434, 1624, 2247, 2600
SchI GAGTC 6 cut(s) 1214, 2134, 2501, 2729, 2871, 3023
ScrFI CCNGG 4 cut(s) 384, 647, 705, 2252
SduI GDGCHC 2 cut(s) 81, 2512
SexAI ACCWGGT 2 cut(s) 645, 703
SfaNI GCATC 6 cut(s) 652, 1158, 1180, 1291, 1660, 2966
SfcI CTRYAG 3 cut(s) 627, 1319, 1716
Sfr274I CTCGAG 1 cut(s) 80
Sfr303I CCGCGG 1 cut(s) 1802
SgrBI CCGCGG 1 cut(s) 1802
SinI GGWCC 5 cut(s) 911, 944, 1434, 1624, 2247
SlaI CTCGAG 1 cut(s) 80
SmiMI CAYNNNNRTG 3 cut(s) 1024, 1300, 2111
SmlI CTYRAG 6 cut(s) 80, 545, 748, 869, 2140, 2879
SmoI CTYRAG 6 cut(s) 80, 545, 748, 869, 2140, 2879
SseBI AGGCCT 1 cut(s) 2268
SsiI CCGC 9 cut(s) 335, 613, 670, 781, 938, 1799, 1801, 1819, 2476
SspI AATATT 1 cut(s) 792
SspMI CTAG 5 cut(s) 104, 401, 836, 977, 1923
SstI GAGCTC 1 cut(s) 81
StuI AGGCCT 1 cut(s) 2268
StyD4I CCNGG 4 cut(s) 382, 645, 703, 2250
StyI CCWWGG 5 cut(s) 606, 759, 817, 1078, 1830
TaaI ACNGT 7 cut(s) 63, 458, 1563, 1988, 2208, 2376, 2590
TaiI ACGT 2 cut(s) 33, 903
TaqI TCGA 6 cut(s) 75, 81, 1466, 1958, 2490, 2812
TatI WGTACW 5 cut(s) 425, 801, 865, 2338, 2655
TauI GCSGC 2 cut(s) 338, 672
TfiI GAWTC 9 cut(s) 128, 311, 632, 723, 854, 1463, 2023, 2166, 2428
TscAI CASTG 7 cut(s) 100, 1222, 1559, 2374, 2381, 2425, 2454
TseFI GTSAC 4 cut(s) 707, 896, 1572, 2126
TseI GCWGC 8 cut(s) 329, 332, 357, 1145, 1360, 1412, 2575, 3041
Tsp45I GTSAC 4 cut(s) 707, 896, 1572, 2126
TspGWI ACGGA 1 cut(s) 512
TspRI CASTG 7 cut(s) 100, 1222, 1559, 2374, 2381, 2425, 2454
Tth111I GACNNNGTC 1 cut(s) 29
VpaK11BI GGWCC 5 cut(s) 911, 944, 1434, 1624, 2247
VspI ATTAAT 1 cut(s) 71
XapI RAATTY 7 cut(s) 431, 929, 1340, 1396, 2281, 2329, 2443
XbaI TCTAGA 1 cut(s) 400
XceI RCATGY 3 cut(s) 412, 1424, 2195
XcmI CCANNNNNNNNNTGG 1 cut(s) 140
XhoI CTCGAG 1 cut(s) 80
XmiI GTMKAC 4 cut(s) 1260, 1741, 1983, 2256
XmnI GAANNNNTTC 2 cut(s) 681, 1670
XspI CTAG 5 cut(s) 104, 401, 836, 977, 1923
ZraI GACGTC 2 cut(s) 31, 901
Zsp2I ATGCAT 2 cut(s) 493, 1173
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.