pycom11g04430

Belongs to the peptidase M16 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0000891
Physical Location & Seq
Forward (+)
3432003 .. 3433977
1975 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom11g04430.6

Sequence Viewer

Length: 972 bp
ATGAAGCCTCTGAGTCACTCATCATACTTAAGGCTGCAAGTTCTGTGTCAAACTTTTTATGATGCAGATGAGAAGTTGCATGTTTTGAATGCATTGTCTATTTCTGATTTGAAGTCATTCATTCCCGAGCTCTGTTCCCAGCTATACATTGAGGGCCTTTGCCATGGAAATTTGTTAGAAGAAGAAGCAATTACCCTGTCAAATATATTTAAATTGACTTTTACCGTACCACCACTGCCTATCGAATTGAGGCATAAAGAGCATGTTATTTGTCTTCCTCCTGGTGCTAACCTGATTAGAGATTCCAATGTGAAGAATAAGTCGGAAACGAACTCTGTGATTGAGCTGTATTTTCAAATTGAGCAAGAAGCAGGGATTGAGTCAATCAGATTGAAATCCTTGATAGATCTTTTCGATGAAATTGTGGAGGAACCAGTTTTCAATCAACTAAGGACGAAGGAGCAGCTTGGATATGTTGTCCAGTGTAGCCCACGGGTAACATACCGTGTACTTGGCTTTTGTTTCTGTGTTCAATCGTCTGAGTACGACCCAATCTACTTACAAGGGAGAGTTGATAACTTTATAGAAGGTCTGCAAGAATTGTTGGACGGGCTTGATGATGAATCATTTGAGAATTATAAAAATGGACTACTTGCTAAGCTATTGGAGAAAGACCCGTCCCTCACATATGAAACCAATAGATTTTGGAATCAGATTATCGATAAAAGGTATATGTTCGACCTGTCAAAACGAGAAGCACAAGAACTCAGCAGTATTCACAAGAAGGACGTTATCAACTGGTACAAAATGTATTTGCAACAATCATCTCCCAAGTGTCGTAGACTAGCGATCCGTGTTTGGGGTTGCAACACTAACCCGAAAGAGGCGGGAGCACAACCGAAATCTATCCAAGCCATCGAAGACCCTGCAACATTTAAGAAGTCATCTAAGTTCTATCCTAGCCTTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0003674 GO:0003824 GO:0004175 GO:0004222 GO:0005102 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005777 GO:0005782 GO:0005829 GO:0006508 GO:0006518 GO:0006605 GO:0006625 GO:0006807 GO:0006810 GO:0006886 GO:0006996 GO:0007031 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007169 GO:0007275 GO:0007568 GO:0008104 GO:0008144 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0008270 GO:0008286 GO:0008340 GO:0009056 GO:0009057 GO:0009719 GO:0009725 GO:0009893 GO:0009894 GO:0009896 GO:0009986 GO:0009987 GO:0010033 GO:0010243 GO:0010259 GO:0010604 GO:0010815 GO:0010992 GO:0015031 GO:0015833 GO:0016043 GO:0016787 GO:0017046 GO:0017076 GO:0017144 GO:0019222 GO:0019538 GO:0019725 GO:0022607 GO:0023052 GO:0030163 GO:0030554 GO:0031334 GO:0031907 GO:0031974 GO:0032459 GO:0032461 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0032868 GO:0032869 GO:0032870 GO:0033036 GO:0033218 GO:0033365 GO:0034613 GO:0034641 GO:0035639 GO:0036094 GO:0042176 GO:0042221 GO:0042277 GO:0042562 GO:0042579 GO:0042592 GO:0042737 GO:0042802 GO:0042803 GO:0042886 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043171 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043254 GO:0043434 GO:0043559 GO:0043574 GO:0043603 GO:0043933 GO:0044085 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044421 GO:0044422 GO:0044424 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0045732 GO:0046872 GO:0046907 GO:0046914 GO:0046983 GO:0048518 GO:0048522 GO:0048856 GO:0050435 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051259 GO:0051260 GO:0051603 GO:0051641 GO:0051649 GO:0051716 GO:0060255 GO:0065003 GO:0065007 GO:0065008 GO:0070011 GO:0070013 GO:0070727 GO:0070887 GO:0071310 GO:0071375 GO:0071417 GO:0071495 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072662 GO:0072663 GO:0080090 GO:0097159 GO:0097242 GO:0097367 GO:0140030 GO:0140035 GO:0140036 GO:0140096 GO:1901142 GO:1901143 GO:1901265 GO:1901363 GO:1901564 GO:1901565 GO:1901575 GO:1901652 GO:1901653 GO:1901698 GO:1901699 GO:1901700 GO:1901701
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_M16 Peptidase_M16_C Peptidase_M16_M

Protein Analysis

324

Amino Acids

37.28

Weight (kDa)

5.54

Isoelectric Point (pI)

59.42

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 639
AccI GTMKAC 1 cut(s) 841
AciI CCGC 1 cut(s) 887
AclWI GGATC 1 cut(s) 844
AcsI RAATTY 1 cut(s) 169
AfaI GTAC 4 cut(s) 228, 510, 545, 803
AfiI CCNNNNNNNGG 2 cut(s) 859, 883
AflII CTTAAG 1 cut(s) 28
AgsI TTSAA 6 cut(s) 88, 112, 356, 394, 442, 533
AjnI CCWGG 1 cut(s) 280
AluBI AGCT 5 cut(s) 130, 142, 346, 466, 661
AluI AGCT 5 cut(s) 130, 142, 346, 466, 661
Alw21I GWGCWC 2 cut(s) 132, 895
AlwI GGATC 1 cut(s) 844
Ama87I CYCGRG 1 cut(s) 125
AoxI GGCC 1 cut(s) 154
ApeKI GCWGC 2 cut(s) 34, 463
ApoI RAATTY 1 cut(s) 169
Asp700I GAANNNNTTC 1 cut(s) 116
AspS9I GGNCC 1 cut(s) 154
AvaI CYCGRG 1 cut(s) 125
BanII GRGCYC 1 cut(s) 132
BbsI GAAGAC 2 cut(s) 266, 927
Bbv12I GWGCWC 2 cut(s) 132, 895
BbvI GCAGC 2 cut(s) 21, 475
BccI CCATC 1 cut(s) 923
BcgI CGANNNNNNTGC 2 cut(s) 908, 942
BciT130I CCWGG 1 cut(s) 282
BfaI CTAG 2 cut(s) 845, 960
BfrI CTTAAG 1 cut(s) 28
BglII AGATCT 1 cut(s) 406
BisI GCNGC 2 cut(s) 35, 464
BlpI GCTNAGC 1 cut(s) 657
BlsI GCNGC 2 cut(s) 36, 465
Bme1390I CCNGG 1 cut(s) 282
BmeT110I CYCGRG 1 cut(s) 125
BmgT120I GGNCC 1 cut(s) 154
BmiI GGNNCC 1 cut(s) 432
BmrFI CCNGG 1 cut(s) 282
BmsI GCATC 1 cut(s) 52
BpiI GAAGAC 2 cut(s) 266, 927
Bpu1102I GCTNAGC 1 cut(s) 657
Bsa29I ATCGAT 1 cut(s) 720
BsaBI GATNNNNATC 1 cut(s) 394
BsaJI CCNNGG 2 cut(s) 163, 491
Bsc4I CCNNNNNNNGG 2 cut(s) 859, 883
Bse1I ACTGG 3 cut(s) 434, 481, 803
Bse8I GATNNNNATC 1 cut(s) 394
BseBI CCWGG 1 cut(s) 282
BseCI ATCGAT 1 cut(s) 720
BseDI CCNNGG 2 cut(s) 163, 491
BseJI GATNNNNATC 1 cut(s) 394
BseLI CCNNNNNNNGG 2 cut(s) 859, 883
BseMII CTCAG 2 cut(s) 531, 781
BseNI ACTGG 3 cut(s) 434, 481, 803
BseXI GCAGC 2 cut(s) 21, 475
BseYI CCCAGC 1 cut(s) 138
BshFI GGCC 1 cut(s) 156
BshVI ATCGAT 1 cut(s) 720
BsiHKAI GWGCWC 2 cut(s) 132, 895
BsiHKCI CYCGRG 1 cut(s) 125
BslFI GGGAC 1 cut(s) 664
BslI CCNNNNNNNGG 2 cut(s) 859, 883
BsmFI GGGAC 1 cut(s) 664
BsmI GAATGC 1 cut(s) 94
BsnI GGCC 1 cut(s) 156
BsoBI CYCGRG 1 cut(s) 125
Bsp1286I GDGCHC 2 cut(s) 132, 895
Bsp143I GATC 2 cut(s) 406, 849
Bsp1720I GCTNAGC 1 cut(s) 657
Bsp19I CCATGG 1 cut(s) 163
BspACI CCGC 1 cut(s) 887
BspANI GGCC 1 cut(s) 156
BspCNI CTCAG 2 cut(s) 532, 780
BspDI ATCGAT 1 cut(s) 720
BspLI GGNNCC 1 cut(s) 432
BspPI GGATC 1 cut(s) 844
BspTI CTTAAG 1 cut(s) 28
BsrI ACTGG 3 cut(s) 434, 481, 803
BssECI CCNNGG 2 cut(s) 163, 491
BssMI GATC 2 cut(s) 406, 849
BssT1I CCWWGG 1 cut(s) 163
Bst2UI CCWGG 1 cut(s) 282
Bst4CI ACNGT 2 cut(s) 226, 506
BstAFI CTTAAG 1 cut(s) 28
BstDEI CTNAG 6 cut(s) 11, 449, 540, 657, 767, 948
BstDSI CCRYGG 2 cut(s) 163, 491
BstKTI GATC 2 cut(s) 409, 852
BstMBI GATC 2 cut(s) 406, 849
BstMWI GCNNNNNNNGC 1 cut(s) 259
BstNI CCWGG 1 cut(s) 282
BstNSI RCATGY 2 cut(s) 83, 266
BstSCI CCNGG 1 cut(s) 280
BstV1I GCAGC 2 cut(s) 21, 475
BstV2I GAAGAC 2 cut(s) 266, 927
BstX2I RGATCY 1 cut(s) 406
BstYI RGATCY 1 cut(s) 406
Bsu15I ATCGAT 1 cut(s) 720
BsuRI GGCC 1 cut(s) 156
BsuTUI ATCGAT 1 cut(s) 720
BtgI CCRYGG 2 cut(s) 163, 491
BtsI GCAGTG 1 cut(s) 233
BtsIMutI CAGTG 2 cut(s) 233, 488
Cfr13I GGNCC 1 cut(s) 154
ClaI ATCGAT 1 cut(s) 720
Csp6I GTAC 4 cut(s) 227, 509, 544, 802
CviAII CATG 3 cut(s) 80, 164, 263
CviQI GTAC 4 cut(s) 227, 509, 544, 802
DdeI CTNAG 6 cut(s) 11, 449, 540, 657, 767, 948
DpnI GATC 2 cut(s) 408, 851
DpnII GATC 2 cut(s) 406, 849
DraI TTTAAA 1 cut(s) 211
Ecl136II GAGCTC 1 cut(s) 130
Eco130I CCWWGG 1 cut(s) 163
Eco24I GRGCYC 1 cut(s) 132
Eco53kI GAGCTC 1 cut(s) 130
Eco88I CYCGRG 1 cut(s) 125
EcoICRI GAGCTC 1 cut(s) 130
EcoO109I RGGNCCY 1 cut(s) 154
EcoRII CCWGG 1 cut(s) 280
EcoT14I CCWWGG 1 cut(s) 163
EcoT22I ATGCAT 1 cut(s) 94
EcoT38I GRGCYC 1 cut(s) 132
ErhI CCWWGG 1 cut(s) 163
FaeI CATG 3 cut(s) 83, 167, 266
FaqI GGGAC 1 cut(s) 664
FatI CATG 3 cut(s) 79, 163, 262
FauI CCCGC 1 cut(s) 880
FauNDI CATATG 1 cut(s) 688
FblI GTMKAC 1 cut(s) 841
Fnu4HI GCNGC 2 cut(s) 35, 464
FriOI GRGCYC 1 cut(s) 132
Fsp4HI GCNGC 2 cut(s) 35, 464
FspBI CTAG 2 cut(s) 845, 960
GluI GCNGC 2 cut(s) 35, 464
GsaI CCCAGC 1 cut(s) 142
HaeIII GGCC 1 cut(s) 156
Hin1II CATG 3 cut(s) 83, 167, 266
HinfI GANTC 5 cut(s) 13, 302, 380, 623, 709
Hpy166II GTNNAC 2 cut(s) 509, 842
Hpy188I TCNGA 6 cut(s) 12, 106, 325, 389, 541, 714
Hpy188III TCNNGA 2 cut(s) 125, 969
Hpy8I GTNNAC 2 cut(s) 509, 842
HpyAV CCTTC 3 cut(s) 451, 581, 778
HpyCH4III ACNGT 2 cut(s) 226, 506
HpyCH4IV ACGT 1 cut(s) 789
HpyCH4V TGCA 8 cut(s) 37, 65, 79, 92, 595, 817, 867, 929
HpyF10VI GCNNNNNNNGC 1 cut(s) 259
HpyF3I CTNAG 6 cut(s) 11, 449, 540, 657, 767, 948
HpySE526I ACGT 1 cut(s) 789
Hsp92II CATG 3 cut(s) 83, 167, 266
Kzo9I GATC 2 cut(s) 406, 849
LmnI GCTCC 2 cut(s) 460, 890
Lsp1109I GCAGC 2 cut(s) 21, 475
LweI GCATC 1 cut(s) 52
MaeI CTAG 2 cut(s) 845, 960
MaeII ACGT 1 cut(s) 789
MaeIII GTNAC 2 cut(s) 14, 496
MalI GATC 2 cut(s) 408, 851
MboI GATC 2 cut(s) 406, 849
MboII GAAGA 5 cut(s) 191, 194, 266, 325, 932
MflI RGATCY 1 cut(s) 406
MhlI GDGCHC 2 cut(s) 132, 895
MluCI AATT 8 cut(s) 169, 189, 212, 245, 357, 420, 599, 634
MlyI GAGTC 2 cut(s) 22, 389
MmeI TCCRAC 2 cut(s) 303, 585
MnlI CCTC 7 cut(s) 18, 145, 243, 288, 421, 692, 877
Mph1103I ATGCAT 1 cut(s) 94
MroXI GAANNNNTTC 1 cut(s) 116
MseI TTAA 3 cut(s) 29, 210, 936
MspCI CTTAAG 1 cut(s) 28
MspR9I CCNGG 1 cut(s) 282
Mva1269I GAATGC 1 cut(s) 94
MvaI CCWGG 1 cut(s) 282
MwoI GCNNNNNNNGC 1 cut(s) 259
NcoI CCATGG 1 cut(s) 163
NdeI CATATG 1 cut(s) 688
NdeII GATC 2 cut(s) 406, 849
NlaIII CATG 3 cut(s) 83, 167, 266
NlaIV GGNNCC 1 cut(s) 432
NmuCI GTSAC 1 cut(s) 14
NsiI ATGCAT 1 cut(s) 94
NspI RCATGY 2 cut(s) 83, 266
PctI GAATGC 1 cut(s) 94
PdmI GAANNNNTTC 1 cut(s) 116
PfeI GAWTC 3 cut(s) 302, 623, 709
PkrI GCNGC 2 cut(s) 36, 465
PleI GAGTC 2 cut(s) 21, 388
PpsI GAGTC 2 cut(s) 21, 388
PsiI TTATAA 1 cut(s) 639
Psp124BI GAGCTC 1 cut(s) 132
Psp6I CCWGG 1 cut(s) 280
PspFI CCCAGC 1 cut(s) 138
PspGI CCWGG 1 cut(s) 280
PspN4I GGNNCC 1 cut(s) 432
PspPI GGNCC 1 cut(s) 154
PsuI RGATCY 1 cut(s) 406
RsaI GTAC 4 cut(s) 228, 510, 545, 803
RsaNI GTAC 4 cut(s) 227, 509, 544, 802
SacI GAGCTC 1 cut(s) 132
SaqAI TTAA 3 cut(s) 29, 210, 936
SatI GCNGC 2 cut(s) 35, 464
Sau3AI GATC 2 cut(s) 406, 849
Sau96I GGNCC 1 cut(s) 154
SchI GAGTC 2 cut(s) 22, 389
ScrFI CCNGG 1 cut(s) 282
SduI GDGCHC 2 cut(s) 132, 895
SfaNI GCATC 1 cut(s) 52
SmiI ATTTAAAT 1 cut(s) 211
SmlI CTYRAG 1 cut(s) 28
SmoI CTYRAG 1 cut(s) 28
Sse9I AATT 8 cut(s) 169, 189, 212, 245, 357, 420, 599, 634
SsiI CCGC 1 cut(s) 887
SspMI CTAG 2 cut(s) 845, 960
SstI GAGCTC 1 cut(s) 132
StyD4I CCNGG 1 cut(s) 280
StyI CCWWGG 1 cut(s) 163
SwaI ATTTAAAT 1 cut(s) 211
TaaI ACNGT 2 cut(s) 226, 506
TaiI ACGT 1 cut(s) 792
TaqI TCGA 5 cut(s) 243, 414, 720, 738, 918
TasI AATT 8 cut(s) 169, 189, 212, 245, 357, 420, 599, 634
TatI WGTACW 1 cut(s) 508
TfiI GAWTC 3 cut(s) 302, 623, 709
Tru1I TTAA 3 cut(s) 29, 210, 936
Tru9I TTAA 3 cut(s) 29, 210, 936
TscAI CASTG 2 cut(s) 240, 488
TseFI GTSAC 1 cut(s) 14
TseI GCWGC 2 cut(s) 34, 463
Tsp45I GTSAC 1 cut(s) 14
TspDTI ATGAA 5 cut(s) 17, 109, 432, 636, 705
TspGWI ACGGA 1 cut(s) 842
TspRI CASTG 2 cut(s) 240, 488
Vha464I CTTAAG 1 cut(s) 28
XapI RAATTY 1 cut(s) 169
XceI RCATGY 2 cut(s) 83, 266
XmiI GTMKAC 1 cut(s) 841
XmnI GAANNNNTTC 1 cut(s) 116
XspI CTAG 2 cut(s) 845, 960
Zsp2I ATGCAT 1 cut(s) 94
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.