MD11G1054400.v1.1

Belongs to the peptidase M16 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0000891
Physical Location & Seq
Forward (+)
4630284 .. 4639175
8892 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1054400.v1.1.491

Sequence Viewer

Length: 3105 bp
ATGGCTCGCTGCACTTTCAAATCGGACGATATCATAATAAAGTCCCCGAATGACAGACGATTATACAGGTTAATCGAGCTCGAGAACGGCCTCTCTGCATTGCTTGTTCACGATCCTGAGATTTACCCAGAAGGGCCGCCGGAGAACTCCAAGTCCGTCGAGCACCGCGAAGCTGAAGAGGAAGAAGAAGATGATGATATGGACGACGAGGATGGGGAGGAAGGGGAAGACGGTGAAGGAGAGGACGATGAAGAGGACGATGACGATGAAGATGAAGAAGCGGAAGGCAGAGATGGTGAACTGAAGAAGAAGGGGAAAGGAGGAGATTCTCATACTAAGAAAGCAGCAGCAGCAATGTGCGTTGGAATAGGAAGCTTCTCCGACCCTTTTGAAGCACAGGGGCTTGCGCATTTTCTAGAACACATGCTCTTCATGGGGAGTACAGAGTTTCCAGATGAAAATGAGTATGATAGTTACTTGTCCAAGCATGGAGGTTCATCAAATGCATACACAGAAGCAGAGCATACCTGCTACCATTTTGAAGTGAAGCCAGAGTTTCTTAAGGGTGCCTTGAGAAGATTTTCCCAGTTCTTTGTTTCACCTCTAGTGAAAATTGAAGCGATGGAACGAGAGGTACAAGCTATAGATTCTGAATTTAACCAGGTTCTGCAGAATGATTCTTGCCGCCTTGAACAAATTCAGTGTCATACATCCGCACCTGGTCACCCATTTAATAGGTTCTGTTGGGGAAATAAGAAGAGCTTGGTTGATGCAATGGAAAAAGGGATCAACTTGCGAGAACAAATACTAAAATTGTACAAGGATTATTACCATGGTGGACTAATGAAGCTAGTTGTCATTGGTGGAGAATCTCTTGATGTACTTGAGGATTGGGTTGTGGAATTGTATGGTAATGTCAAGAAAGGCCCCCAAGTAAAACTGGAGTTCAAGGCTGAAGGTCCTATCTGGAAAGTTGGCAAACTATATAGGCTAGAGGCTGTTAGAGATGTTAACATACTCAACCTTACATGGACATTTCCATGTCTTCATCAAGACTATTTGAAGAAACCAGAAGATTATTTAGCTCATCTACTTGGGCATGAGGGCCGGGGAAGTTTGCATTTTTACCTCAAAACCAGAGGGTGGGCAACATCTTTGTCTGCTGGTGTTGGGGACGAGGGGATGCAACGATCTTCTGTGGCTTATGTCTTCCGCATGTCCATACACCTCACTGACTCTGGATTGGAAAAGATTTCCGAAATAATTTGCTATGTCTACCAGTACATTAAGTTACTGCATCAAGTGTCTCCACAAGAATGGATATTTAGGGAACTTCAAGATATTGGGAATATGGATTTTAGATTCGCAGAGGAGCAGCCCCAAGATGATTATGCTGCAGAACTTGCAGAAAATTTACTACTGTATCCAGCAGAGAACGTTATTTATGGGGACTATGTGTACAAGATTTGGGATGCGGAACTGATAAAATATGTTCTTGGTTTCTTCACACCAGAAAACATGAGGGTTGATGTGGTATCAAAGTCCTCAATTAAGTCAGAAGATTTCCAGTGCGAGCCTTGGTTTGGGTCACATTATACTGAGGAAGATATATCTCCATCTTTGATGGATTTGTGGAAGAATCCCCCAGAAATTGATGACTCATTGTATCTACCTTCAAAGAATGAGTTCATTCCTAGTGATTTTTCGATCCGCTCTGATAACTCGTGCCTTGATCCTGCAAATATATCTTATCCAAGATGTATAATTGATGAACCATTGATGAAGTTCTGGTACAAACTCGATGATTCATTTAAATTTCCGCGGGCAAATACATACTTCCGCATCAATCTGAAGGGTGGATATGCTAATCTGAAGAGTTGTGTTTTGACTGAACTATATATTCTCCTTCTTAAGGATGAGCTGAATGAGATTGTCTATCAGGCCATTGTTGCCAAGCTGGAAACTTCTGTATCTATGTTTAGTGACAAACTGGAGCTGAAGGTCTATGGTTTTAATGATAAACTTCCAGCTCTCTTTTCAAAAGTTTTGGAAACAGCCAAAAGTTTCATGCCGACTGATGATCGTTTTAAGGATGTTAAAGAAGATATGAAGCGAACGTTAAAGAACACCAATATGAAGCCTCTGAGTCACTCATCATACTTAAGGCTGCAAGTTCTGTGTCAAACTTTTTATGATGCAGATGAGAAGTTGCATGTTTTGAATGAATTGTCTATATCTGATTTGAAGTCATTCATTCCCGAGCTCTGTTCCCAGCTATACATTGAGGGCCTTTGCCATGGAAATTTGTTAGAAGAAGAAGCAATTAACCTGTCAAATATATTTAAATCGACTTTTACCGTACCACCACTGCCTATCGAATTGAGGCATAAAGAGCATGTTATTTGTCTTCCTCCTGGTGCTAACCTGATTAGAGATTCCAATGTGAAGAATAAGTCGGAAACGAACTCTGTGATTGAGCTGTATTTTCAAATTGAGCAAGAAGCAGGGATTGAGTCAGTCAGATTGAAATCCTTGATAGATCTTTTCGATGAAATTGTGGAGGAACCACTTTTCAATCAACTAAGGACGAAGGAGCAGCTTGGATATGTTGTCCAGTGTAGCCCACGGGTAACATACCGTGTACTTGGCTTTTGTTTCTGTGTTCAATCGTCTGAGTACGACCCAATATACTTACAAGGGAGAGTTGATAACTTTATAGATGGTCTGCAAGAATTGTTGGACGGGCTTGATGATGAATCATTCGAGAATTATAAAAATGGACTACTTGCTAAGCTATTGGAGAAAGATCCGTCCCTCACATATGAAACCAATAGATTTTGGAATCAGATTATCGATAAAAGGTATATGTTCGACCTGTCAAAACGAGAAGCAGAAGAACTCAGCAGTATTCACAAGAAGGACGTTATCAACTGGTACAAAATGTATTTGCAACAATCATCTCCCAAGTGTCGTAGACTAGCGATCCGTGTTTGGGGTTGCAACACTAACCCGAAAGAGGCGGAAGCACAACCGAAATCCATCCAAGCCATCGAAGACCCTGCAACGTTTAAGAAGTCATCTAAGTTCTATCCTAGCCTTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0003674 GO:0003824 GO:0004175 GO:0004222 GO:0005102 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005777 GO:0005782 GO:0005829 GO:0006508 GO:0006518 GO:0006605 GO:0006625 GO:0006807 GO:0006810 GO:0006886 GO:0006996 GO:0007031 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007169 GO:0007275 GO:0007568 GO:0008104 GO:0008144 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0008270 GO:0008286 GO:0008340 GO:0009056 GO:0009057 GO:0009719 GO:0009725 GO:0009893 GO:0009894 GO:0009896 GO:0009986 GO:0009987 GO:0010033 GO:0010243 GO:0010259 GO:0010604 GO:0010815 GO:0010992 GO:0015031 GO:0015833 GO:0016043 GO:0016787 GO:0017046 GO:0017076 GO:0017144 GO:0019222 GO:0019538 GO:0019725 GO:0022607 GO:0023052 GO:0030163 GO:0030554 GO:0031334 GO:0031907 GO:0031974 GO:0032459 GO:0032461 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0032868 GO:0032869 GO:0032870 GO:0033036 GO:0033218 GO:0033365 GO:0034613 GO:0034641 GO:0035639 GO:0036094 GO:0042176 GO:0042221 GO:0042277 GO:0042562 GO:0042579 GO:0042592 GO:0042737 GO:0042802 GO:0042803 GO:0042886 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043171 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043254 GO:0043434 GO:0043559 GO:0043574 GO:0043603 GO:0043933 GO:0044085 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044421 GO:0044422 GO:0044424 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0045732 GO:0046872 GO:0046907 GO:0046914 GO:0046983 GO:0048518 GO:0048522 GO:0048856 GO:0050435 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051259 GO:0051260 GO:0051603 GO:0051641 GO:0051649 GO:0051716 GO:0060255 GO:0065003 GO:0065007 GO:0065008 GO:0070011 GO:0070013 GO:0070727 GO:0070887 GO:0071310 GO:0071375 GO:0071417 GO:0071495 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072662 GO:0072663 GO:0080090 GO:0097159 GO:0097242 GO:0097367 GO:0140030 GO:0140035 GO:0140036 GO:0140096 GO:1901142 GO:1901143 GO:1901265 GO:1901363 GO:1901564 GO:1901565 GO:1901575 GO:1901652 GO:1901653 GO:1901698 GO:1901699 GO:1901700 GO:1901701
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_M16 Peptidase_M16_C Peptidase_M16_M

Protein Analysis

1035

Amino Acids

119.08

Weight (kDa)

4.82

Isoelectric Point (pI)

43.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M16 PF00675 110 - 226 2.5e-31 Insulinase (Peptidase family M16)
Peptidase_M16_C PF05193 266 - 446 4.1e-17 Peptidase M16 inactive domain
Peptidase_M16_M PF16187 453 - 738 9.1e-87 Middle or third domain of peptidase_M16
Peptidase_M16_C PF05193 743 - 928 5.7e-15 Peptidase M16 inactive domain
PqqF-like_C_4 PF22456 848 - 947 8.1e-26 PQQ synthase PqqF-like, C-terminal lobe domain 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2772
Acc16I TGCGCA 1 cut(s) 408
Acc36I ACCTGC 1 cut(s) 536
AccB1I GGYRCC 1 cut(s) 566
AccB7I CCANNNNNTGG 1 cut(s) 1143
AccBSI CCGCTC 1 cut(s) 1713
AccI GTMKAC 2 cut(s) 1275, 2974
AccII CGCG 2 cut(s) 168, 1822
AclI AACGTT 3 cut(s) 1437, 2117, 3065
AclWI GGATC 6 cut(s) 107, 794, 1702, 1727, 2801, 2977
AcsI RAATTY 5 cut(s) 653, 696, 1411, 1814, 2302
AcuI CTGAAG 6 cut(s) 195, 323, 975, 1871, 1892, 2018
AfiI CCNNNNNNNGG 5 cut(s) 1143, 1583, 1912, 2992, 3016
AflII CTTAAG 3 cut(s) 560, 1910, 2161
AjnI CCWGG 3 cut(s) 660, 718, 2413
AloI GAACNNNNNNTCC 2 cut(s) 137, 169
Alw21I GWGCWC 3 cut(s) 81, 165, 2265
Alw26I GTCTC 1 cut(s) 1311
AlwI GGATC 6 cut(s) 107, 794, 1702, 1727, 2801, 2977
AlwNI CAGNNNCTG 1 cut(s) 667
Ama87I CYCGRG 2 cut(s) 80, 2258
AoxI GGCC 6 cut(s) 88, 134, 925, 1105, 1941, 2287
ApeKI GCWGC 8 cut(s) 9, 344, 347, 350, 1375, 1394, 2167, 2596
ApoI RAATTY 5 cut(s) 653, 696, 1411, 1814, 2302
ArsI GACNNNNNNTTYG 2 cut(s) 688, 720
Asp700I GAANNNNTTC 4 cut(s) 580, 696, 1685, 2249
AspLEI GCGC 1 cut(s) 409
AspS9I GGNCC 5 cut(s) 134, 926, 959, 1105, 2287
AsuC2I CCSGG 1 cut(s) 1109
AsuHPI GGTGA 4 cut(s) 245, 308, 591, 716
AvaI CYCGRG 2 cut(s) 80, 2258
AvaII GGWCC 1 cut(s) 959
BaeI ACNNNNGTAYC 4 cut(s) 1406, 1439, 1953, 1986
BanI GGYRCC 1 cut(s) 566
BanII GRGCYC 2 cut(s) 81, 2265
BarI GAAGNNNNNNTAC 2 cut(s) 1775, 1807
BauI CACGAG 1 cut(s) 1723
BbsI GAAGAC 5 cut(s) 234, 1037, 1201, 2399, 3060
Bbv12I GWGCWC 3 cut(s) 81, 165, 2265
BbvI GCAGC 7 cut(s) 356, 359, 362, 1381, 1387, 2154, 2608
BccI CCATC 8 cut(s) 206, 287, 616, 1618, 1624, 2714, 3047, 3056
BceAI ACGGC 1 cut(s) 103
BcgI CGANNNNNNTGC 2 cut(s) 3041, 3075
BciT130I CCWGG 3 cut(s) 662, 720, 2415
BciVI GTATCC 1 cut(s) 1434
BcnI CCSGG 1 cut(s) 1109
BcoDI GTCTC 1 cut(s) 1311
BfaI CTAG 7 cut(s) 416, 605, 851, 992, 1695, 2978, 3093
BfmI CTRYAG 3 cut(s) 642, 668, 1395
BfrI CTTAAG 3 cut(s) 560, 1910, 2161
BfuAI ACCTGC 1 cut(s) 536
BfuI GTATCC 1 cut(s) 1434
BglII AGATCT 1 cut(s) 2539
BlpI GCTNAGC 1 cut(s) 2790
Bme1390I CCNGG 4 cut(s) 662, 720, 1109, 2415
Bme18I GGWCC 1 cut(s) 959
BmeT110I CYCGRG 2 cut(s) 80, 2258
BmgT120I GGNCC 5 cut(s) 134, 926, 959, 1105, 2287
BmiI GGNNCC 3 cut(s) 568, 928, 2565
BmrFI CCNGG 4 cut(s) 662, 720, 1109, 2415
BmrI ACTGGG 1 cut(s) 580
BmsI GCATC 6 cut(s) 760, 1173, 1306, 1462, 1851, 2185
BmuI ACTGGG 1 cut(s) 580
BpiI GAAGAC 5 cut(s) 234, 1037, 1201, 2399, 3060
BpmI CTGGAG 2 cut(s) 962, 2012
Bpu1102I GCTNAGC 1 cut(s) 2790
BpuEI CTTGAG 2 cut(s) 592, 905
BpuMI CCSGG 1 cut(s) 1109
Bsa29I ATCGAT 1 cut(s) 2853
BsaBI GATNNNNATC 1 cut(s) 2527
BsaJI CCNNGG 6 cut(s) 832, 1108, 1577, 1820, 2296, 2624
Bsc4I CCNNNNNNNGG 5 cut(s) 1143, 1583, 1912, 2992, 3016
Bse1I ACTGG 7 cut(s) 586, 945, 1279, 1567, 1995, 2614, 2936
Bse3DI GCAATG 3 cut(s) 98, 360, 780
Bse8I GATNNNNATC 1 cut(s) 2527
BseBI CCWGG 3 cut(s) 662, 720, 2415
BseCI ATCGAT 1 cut(s) 2853
BseDI CCNNGG 6 cut(s) 832, 1108, 1577, 1820, 2296, 2624
BseGI GGATG 7 cut(s) 217, 710, 1188, 1477, 1921, 2098, 3039
BseJI GATNNNNATC 1 cut(s) 2527
BseLI CCNNNNNNNGG 5 cut(s) 1143, 1583, 1912, 2992, 3016
BseMI GCAATG 3 cut(s) 98, 360, 780
BseMII CTCAG 5 cut(s) 108, 1590, 2135, 2664, 2914
BseNI ACTGG 7 cut(s) 586, 945, 1279, 1567, 1995, 2614, 2936
BseRI GAGGAG 2 cut(s) 336, 1385
BseXI GCAGC 7 cut(s) 356, 359, 362, 1381, 1387, 2154, 2608
BseYI CCCAGC 1 cut(s) 2271
Bsh1236I CGCG 2 cut(s) 168, 1822
BshFI GGCC 6 cut(s) 90, 136, 927, 1107, 1943, 2289
BshNI GGYRCC 1 cut(s) 566
BshVI ATCGAT 1 cut(s) 2853
BsiHKAI GWGCWC 3 cut(s) 81, 165, 2265
BsiHKCI CYCGRG 2 cut(s) 80, 2258
BsiSI CCGG 2 cut(s) 140, 1108
BslFI GGGAC 4 cut(s) 28, 1187, 1463, 2797
BslI CCNNNNNNNGG 5 cut(s) 1143, 1583, 1912, 2992, 3016
BsmAI GTCTC 1 cut(s) 1311
BsmFI GGGAC 4 cut(s) 28, 1187, 1463, 2797
BsnI GGCC 6 cut(s) 90, 136, 927, 1107, 1943, 2289
BsoBI CYCGRG 2 cut(s) 80, 2258
Bsp1286I GDGCHC 3 cut(s) 81, 165, 2265
Bsp1407I TGTACA 2 cut(s) 816, 1458
Bsp143I GATC 9 cut(s) 112, 786, 1190, 1707, 1732, 2080, 2539, 2806, 2982
Bsp1720I GCTNAGC 1 cut(s) 2790
Bsp19I CCATGG 2 cut(s) 832, 2296
BspANI GGCC 6 cut(s) 90, 136, 927, 1107, 1943, 2289
BspCNI CTCAG 5 cut(s) 109, 1591, 2136, 2665, 2913
BspDI ATCGAT 1 cut(s) 2853
BspFNI CGCG 2 cut(s) 168, 1822
BspLI GGNNCC 3 cut(s) 568, 928, 2565
BspMAI CTGCAG 2 cut(s) 672, 1399
BspMI ACCTGC 1 cut(s) 536
BspPI GGATC 6 cut(s) 107, 794, 1702, 1727, 2801, 2977
BspQI GCTCTTC 2 cut(s) 434, 752
BspT107I GGYRCC 1 cut(s) 566
BspTI CTTAAG 3 cut(s) 560, 1910, 2161
BsrBI CCGCTC 1 cut(s) 1713
BsrDI GCAATG 3 cut(s) 98, 360, 780
BsrGI TGTACA 2 cut(s) 816, 1458
BsrI ACTGG 7 cut(s) 586, 945, 1279, 1567, 1995, 2614, 2936
BssECI CCNNGG 6 cut(s) 832, 1108, 1577, 1820, 2296, 2624
BssMI GATC 9 cut(s) 112, 786, 1190, 1707, 1732, 2080, 2539, 2806, 2982
BssSI CACGAG 1 cut(s) 1723
BssT1I CCWWGG 3 cut(s) 832, 1577, 2296
Bst2BI CACGAG 1 cut(s) 1723
Bst2UI CCWGG 3 cut(s) 662, 720, 2415
Bst4CI ACNGT 4 cut(s) 233, 1422, 2359, 2639
Bst6I CTCTTC 5 cut(s) 171, 246, 434, 752, 1868
BstAFI CTTAAG 3 cut(s) 560, 1910, 2161
BstAPI GCANNNNNTGC 1 cut(s) 1403
BstAUI TGTACA 2 cut(s) 816, 1458
BstC8I GCNNGC 4 cut(s) 7, 405, 1574, 1824
BstDEI CTNAG 9 cut(s) 117, 336, 1599, 2144, 2582, 2673, 2790, 2900, 3081
BstDSI CCRYGG 4 cut(s) 832, 1820, 2296, 2624
BstEII GGTNACC 1 cut(s) 722
BstENI CCTNNNNNAGG 1 cut(s) 1910
BstF5I GGATG 7 cut(s) 217, 710, 1188, 1477, 1921, 2098, 3039
BstFNI CGCG 2 cut(s) 168, 1822
BstHHI GCGC 1 cut(s) 409
BstKTI GATC 9 cut(s) 115, 789, 1193, 1710, 1735, 2083, 2542, 2809, 2985
BstMAI GTCTC 1 cut(s) 1311
BstMBI GATC 9 cut(s) 112, 786, 1190, 1707, 1732, 2080, 2539, 2806, 2982
BstMWI GCNNNNNNNGC 4 cut(s) 350, 1403, 1949, 2392
BstNI CCWGG 3 cut(s) 662, 720, 2415
BstNSI RCATGY 4 cut(s) 427, 1219, 2216, 2399
BstPI GGTNACC 1 cut(s) 722
BstSCI CCNGG 4 cut(s) 660, 718, 1107, 2413
BstSFI CTRYAG 3 cut(s) 642, 668, 1395
BstUI CGCG 2 cut(s) 168, 1822
BstV1I GCAGC 7 cut(s) 356, 359, 362, 1381, 1387, 2154, 2608
BstV2I GAAGAC 5 cut(s) 234, 1037, 1201, 2399, 3060
BstX2I RGATCY 2 cut(s) 2539, 2806
BstXI CCANNNNNNTGG 1 cut(s) 1317
BstYI RGATCY 2 cut(s) 2539, 2806
Bsu15I ATCGAT 1 cut(s) 2853
BsuI GTATCC 1 cut(s) 1434
BsuRI GGCC 6 cut(s) 90, 136, 927, 1107, 1943, 2289
BsuTUI ATCGAT 1 cut(s) 2853
BtgI CCRYGG 4 cut(s) 832, 1820, 2296, 2624
BtgZI GCGATG 1 cut(s) 635
BtsCI GGATG 7 cut(s) 217, 710, 1188, 1477, 1921, 2098, 3039
BtsI GCAGTG 1 cut(s) 2366
BtsIMutI CAGTG 5 cut(s) 707, 1230, 1574, 2366, 2621
BveI ACCTGC 1 cut(s) 536
Cac8I GCNNGC 4 cut(s) 7, 405, 1574, 1824
CaiI CAGNNNCTG 1 cut(s) 667
CfoI GCGC 1 cut(s) 409
Cfr13I GGNCC 5 cut(s) 134, 926, 959, 1105, 2287
Cfr42I CCGCGG 1 cut(s) 1823
ClaI ATCGAT 1 cut(s) 2853
CsiI ACCWGGT 2 cut(s) 660, 718
DdeI CTNAG 9 cut(s) 117, 336, 1599, 2144, 2582, 2673, 2790, 2900, 3081
DpnI GATC 9 cut(s) 114, 788, 1192, 1709, 1734, 2082, 2541, 2808, 2984
DpnII GATC 9 cut(s) 112, 786, 1190, 1707, 1732, 2080, 2539, 2806, 2982
DraI TTTAAA 2 cut(s) 1813, 2344
Eam1104I CTCTTC 5 cut(s) 171, 246, 434, 752, 1868
EarI CTCTTC 5 cut(s) 171, 246, 434, 752, 1868
EciI GGCGGA 1 cut(s) 3035
Ecl136II GAGCTC 2 cut(s) 79, 2263
Eco130I CCWWGG 3 cut(s) 832, 1577, 2296
Eco24I GRGCYC 2 cut(s) 81, 2265
Eco32I GATATC 1 cut(s) 31
Eco47I GGWCC 1 cut(s) 959
Eco53kI GAGCTC 2 cut(s) 79, 2263
Eco57I CTGAAG 6 cut(s) 195, 323, 975, 1871, 1892, 2018
Eco88I CYCGRG 2 cut(s) 80, 2258
Eco91I GGTNACC 1 cut(s) 722
EcoICRI GAGCTC 2 cut(s) 79, 2263
EcoNI CCTNNNNNAGG 1 cut(s) 1910
EcoO109I RGGNCCY 3 cut(s) 926, 959, 2287
EcoO65I GGTNACC 1 cut(s) 722
EcoRII CCWGG 3 cut(s) 660, 718, 2413
EcoRV GATATC 1 cut(s) 31
EcoT14I CCWWGG 3 cut(s) 832, 1577, 2296
EcoT22I ATGCAT 1 cut(s) 508
EcoT38I GRGCYC 2 cut(s) 81, 2265
ErhI CCWWGG 3 cut(s) 832, 1577, 2296
FalI AAGNNNNNCTT 4 cut(s) 554, 586, 746, 778
FaqI GGGAC 4 cut(s) 28, 1187, 1463, 2797
FauI CCCGC 1 cut(s) 1815
FauNDI CATATG 1 cut(s) 2821
FblI GTMKAC 2 cut(s) 1275, 2974
FokI GGATG 7 cut(s) 224, 697, 1195, 1484, 1928, 2105, 3026
FriOI GRGCYC 2 cut(s) 81, 2265
FspBI CTAG 7 cut(s) 416, 605, 851, 992, 1695, 2978, 3093
FspI TGCGCA 1 cut(s) 408
GlaI GCGC 1 cut(s) 408
GsaI CCCAGC 1 cut(s) 2275
GsuI CTGGAG 2 cut(s) 962, 2012
HaeIII GGCC 6 cut(s) 90, 136, 927, 1107, 1943, 2289
HapII CCGG 2 cut(s) 140, 1108
HhaI GCGC 1 cut(s) 409
Hin6I GCGC 1 cut(s) 407
HinP1I GCGC 1 cut(s) 407
HincII GTYRAC 1 cut(s) 1012
HindII GTYRAC 1 cut(s) 1012
HindIII AAGCTT 1 cut(s) 373
HpaI GTTAAC 1 cut(s) 1012
HpaII CCGG 2 cut(s) 140, 1108
HphI GGTGA 4 cut(s) 245, 308, 591, 716
Hpy166II GTNNAC 8 cut(s) 109, 299, 839, 1012, 1276, 1459, 2642, 2975
Hpy8I GTNNAC 8 cut(s) 109, 299, 839, 1012, 1276, 1459, 2642, 2975
Hpy99I CGWCG 2 cut(s) 161, 209
HpyCH4III ACNGT 4 cut(s) 233, 1422, 2359, 2639
HpyCH4IV ACGT 4 cut(s) 1437, 2117, 2922, 3065
HpyF10VI GCNNNNNNNGC 4 cut(s) 350, 1403, 1949, 2392
HpyF3I CTNAG 9 cut(s) 117, 336, 1599, 2144, 2582, 2673, 2790, 2900, 3081
HpySE526I ACGT 4 cut(s) 1437, 2117, 2922, 3065
HspAI GCGC 1 cut(s) 407
KspAI GTTAAC 1 cut(s) 1012
KspI CCGCGG 1 cut(s) 1823
Kzo9I GATC 9 cut(s) 112, 786, 1190, 1707, 1732, 2080, 2539, 2806, 2982
LguI GCTCTTC 2 cut(s) 434, 752
LmnI GCTCC 3 cut(s) 1372, 1993, 2593
Lsp1109I GCAGC 7 cut(s) 356, 359, 362, 1381, 1387, 2154, 2608
LweI GCATC 6 cut(s) 760, 1173, 1306, 1462, 1851, 2185
MabI ACCWGGT 2 cut(s) 660, 718
MaeI CTAG 7 cut(s) 416, 605, 851, 992, 1695, 2978, 3093
MaeII ACGT 4 cut(s) 1437, 2117, 2922, 3065
MaeIII GTNAC 7 cut(s) 473, 722, 1290, 1587, 1982, 2147, 2629
MalI GATC 9 cut(s) 114, 788, 1192, 1709, 1734, 2082, 2541, 2808, 2984
MbiI CCGCTC 1 cut(s) 1713
MboI GATC 9 cut(s) 112, 786, 1190, 1707, 1732, 2080, 2539, 2806, 2982
MflI RGATCY 2 cut(s) 2539, 2806
MhlI GDGCHC 3 cut(s) 81, 165, 2265
MlyI GAGTC 4 cut(s) 1229, 1652, 2155, 2522
MmeI TCCRAC 4 cut(s) 343, 405, 2436, 2718
Mph1103I ATGCAT 1 cut(s) 508
MroXI GAANNNNTTC 4 cut(s) 580, 696, 1685, 2249
MslI CAYNNNNRTG 3 cut(s) 1039, 1315, 2132
MspA1I CMGCKG 1 cut(s) 1822
MspCI CTTAAG 3 cut(s) 560, 1910, 2161
MspI CCGG 2 cut(s) 140, 1108
MspR9I CCNGG 4 cut(s) 662, 720, 1109, 2415
MvaI CCWGG 3 cut(s) 662, 720, 2415
MvnI CGCG 2 cut(s) 168, 1822
MwoI GCNNNNNNNGC 4 cut(s) 350, 1403, 1949, 2392
NciI CCSGG 1 cut(s) 1109
NcoI CCATGG 2 cut(s) 832, 2296
NdeI CATATG 1 cut(s) 2821
NdeII GATC 9 cut(s) 112, 786, 1190, 1707, 1732, 2080, 2539, 2806, 2982
NlaIV GGNNCC 3 cut(s) 568, 928, 2565
NmuCI GTSAC 4 cut(s) 722, 1587, 1982, 2147
NsbI TGCGCA 1 cut(s) 408
NsiI ATGCAT 1 cut(s) 508
NspI RCATGY 4 cut(s) 427, 1219, 2216, 2399
PaeR7I CTCGAG 1 cut(s) 80
PciSI GCTCTTC 2 cut(s) 434, 752
PcsI WCGNNNNNNNCGW 1 cut(s) 165
PdmI GAANNNNTTC 4 cut(s) 580, 696, 1685, 2249
PflMI CCANNNNNTGG 1 cut(s) 1143
PleI GAGTC 4 cut(s) 1229, 1652, 2154, 2521
PpsI GAGTC 4 cut(s) 1229, 1652, 2154, 2521
PpuMI RGGWCCY 1 cut(s) 959
PsiI TTATAA 1 cut(s) 2772
Psp124BI GAGCTC 2 cut(s) 81, 2265
Psp1406I AACGTT 3 cut(s) 1437, 2117, 3065
Psp5II RGGWCCY 1 cut(s) 959
Psp6I CCWGG 3 cut(s) 660, 718, 2413
PspEI GGTNACC 1 cut(s) 722
PspFI CCCAGC 1 cut(s) 2271
PspGI CCWGG 3 cut(s) 660, 718, 2413
PspN4I GGNNCC 3 cut(s) 568, 928, 2565
PspPI GGNCC 5 cut(s) 134, 926, 959, 1105, 2287
PspPPI RGGWCCY 1 cut(s) 959
PsrI GAACNNNNNNTAC 2 cut(s) 618, 650
PstI CTGCAG 2 cut(s) 672, 1399
PstNI CAGNNNCTG 1 cut(s) 667
PsuI RGATCY 2 cut(s) 2539, 2806
RseI CAYNNNNRTG 3 cut(s) 1039, 1315, 2132
SacI GAGCTC 2 cut(s) 81, 2265
SacII CCGCGG 1 cut(s) 1823
SapI GCTCTTC 2 cut(s) 434, 752
Sau3AI GATC 9 cut(s) 112, 786, 1190, 1707, 1732, 2080, 2539, 2806, 2982
Sau96I GGNCC 5 cut(s) 134, 926, 959, 1105, 2287
SchI GAGTC 4 cut(s) 1229, 1652, 2155, 2522
ScrFI CCNGG 4 cut(s) 662, 720, 1109, 2415
SduI GDGCHC 3 cut(s) 81, 165, 2265
SexAI ACCWGGT 2 cut(s) 660, 718
SfaNI GCATC 6 cut(s) 760, 1173, 1306, 1462, 1851, 2185
SfcI CTRYAG 3 cut(s) 642, 668, 1395
Sfr274I CTCGAG 1 cut(s) 80
Sfr303I CCGCGG 1 cut(s) 1823
SgrBI CCGCGG 1 cut(s) 1823
SinI GGWCC 1 cut(s) 959
SlaI CTCGAG 1 cut(s) 80
SmiI ATTTAAAT 2 cut(s) 1813, 2344
SmiMI CAYNNNNRTG 3 cut(s) 1039, 1315, 2132
SmlI CTYRAG 6 cut(s) 80, 560, 571, 884, 1910, 2161
SmoI CTYRAG 6 cut(s) 80, 560, 571, 884, 1910, 2161
SspMI CTAG 7 cut(s) 416, 605, 851, 992, 1695, 2978, 3093
SstI GAGCTC 2 cut(s) 81, 2265
StyD4I CCNGG 4 cut(s) 660, 718, 1107, 2413
StyI CCWWGG 3 cut(s) 832, 1577, 2296
SwaI ATTTAAAT 2 cut(s) 1813, 2344
TaaI ACNGT 4 cut(s) 233, 1422, 2359, 2639
TaiI ACGT 4 cut(s) 1440, 2120, 2925, 3068
TatI WGTACW 6 cut(s) 440, 816, 880, 1281, 1458, 2641
TauI GCSGC 2 cut(s) 139, 687
TscAI CASTG 5 cut(s) 707, 1237, 1574, 2373, 2621
TseFI GTSAC 4 cut(s) 722, 1587, 1982, 2147
TseI GCWGC 8 cut(s) 9, 344, 347, 350, 1375, 1394, 2167, 2596
Tsp45I GTSAC 4 cut(s) 722, 1587, 1982, 2147
TspGWI ACGGA 3 cut(s) 145, 2799, 2975
TspRI CASTG 5 cut(s) 707, 1237, 1574, 2373, 2621
Van91I CCANNNNNTGG 1 cut(s) 1143
Vha464I CTTAAG 3 cut(s) 560, 1910, 2161
VpaK11BI GGWCC 1 cut(s) 959
XagI CCTNNNNNAGG 1 cut(s) 1910
XapI RAATTY 5 cut(s) 653, 696, 1411, 1814, 2302
XbaI TCTAGA 1 cut(s) 415
XceI RCATGY 4 cut(s) 427, 1219, 2216, 2399
XhoI CTCGAG 1 cut(s) 80
XmiI GTMKAC 2 cut(s) 1275, 2974
XmnI GAANNNNTTC 4 cut(s) 580, 696, 1685, 2249
XspI CTAG 7 cut(s) 416, 605, 851, 992, 1695, 2978, 3093
Zsp2I ATGCAT 1 cut(s) 508
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.