RchiOBHm_Chr1g0333951

Belongs to the peptidase M16 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000891
Physical Location & Seq
Reverse (-)
26073056 .. 26075697
2642 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ56272

Sequence Viewer

Length: 957 bp
ATGAGATTGTTTATCTGGTTATTACCTCATCTCATATTGACAAAAGCCTTTGTGGCTAGTGCTGCCAATCTGGAAACTTTGGTATCTGTATCGACTGACAGGCTGGAGCTAAGGGTCTACGGTTTCAATGACAAGCTTCCAGCTCTGTTGTCAAAGATTCTGAAAACAACCAAAAGTTTCATGCCAATTTCTGATCGTTTTATGAGATTGTATGATGTTGATGAGAAGTTGCACATTTTAAGTGGACTTTCTATTTCTGATGTGAAGTCATTTATTCCTCAGCTTTGGTCCAAGGTGTACATTGAAAGCCTCTGTCATGGAAATTTGTCAGAAAAAGAAGCAATCAGGCTTTCAGATATATTTAAAACAAATTTTGGTGTACAACCACTTCCAATTGAACTGATGCGTAGTGAACAGTGTATTTGTCTTCCTCCTTCTGCTAACCTCATTAGAGATATCAGTGTGAAGAATAAGTCTAAAACAAATTCGCTTCAAATTGAGCGGGAATTGAGGATCGAGTCCCCTAGATTGAGAGCCGTGTTAGATCTTTTCAATGAAATTGTGGAGGAACCACTTTTCAATCAGCTAAGGACGAAGGAGCAGCTTGGTTGTACTGTTCATTGTGGCCCGTGGGTTACTTGCAATACATTTGGATTTTATTTTTGCGTTCAGTCAGCTGAGGACAACCCGATCTACTTGCAAAGGAGAGTTGAGAACTTTATAAATAGACTGGAGGAGTTGTTGCTCGTCAATGACTGGATGATGTCTCTTTTGAGAATTACAGAGCTGGACCAATTGCAAAGCTATTGGAGAAAGATCCATCCCTCCATTATGAAACCAATCGATATTGGTTACAGATTACCGATAAAATGTAGTGTATATATCCTCTCTGCCTGCTGGAAGCTTTGCCTAGACCTTTCTAATGCTCGAGCTCTGAACTATTCAAATAAATGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0003674 GO:0003824 GO:0004175 GO:0004222 GO:0005102 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005777 GO:0005782 GO:0005829 GO:0006508 GO:0006518 GO:0006605 GO:0006625 GO:0006807 GO:0006810 GO:0006886 GO:0006996 GO:0007031 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007169 GO:0007275 GO:0007568 GO:0008104 GO:0008144 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0008270 GO:0008286 GO:0008340 GO:0009056 GO:0009057 GO:0009719 GO:0009725 GO:0009893 GO:0009894 GO:0009896 GO:0009986 GO:0009987 GO:0010033 GO:0010243 GO:0010259 GO:0010604 GO:0010815 GO:0010992 GO:0015031 GO:0015833 GO:0016043 GO:0016787 GO:0017046 GO:0017076 GO:0017144 GO:0019222 GO:0019538 GO:0019725 GO:0022607 GO:0023052 GO:0030163 GO:0030554 GO:0031334 GO:0031907 GO:0031974 GO:0032459 GO:0032461 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0032868 GO:0032869 GO:0032870 GO:0033036 GO:0033218 GO:0033365 GO:0034613 GO:0034641 GO:0035639 GO:0036094 GO:0042176 GO:0042221 GO:0042277 GO:0042562 GO:0042579 GO:0042592 GO:0042737 GO:0042802 GO:0042803 GO:0042886 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043171 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043254 GO:0043434 GO:0043559 GO:0043574 GO:0043603 GO:0043933 GO:0044085 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044421 GO:0044422 GO:0044424 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0045732 GO:0046872 GO:0046907 GO:0046914 GO:0046983 GO:0048518 GO:0048522 GO:0048856 GO:0050435 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051259 GO:0051260 GO:0051603 GO:0051641 GO:0051649 GO:0051716 GO:0060255 GO:0065003 GO:0065007 GO:0065008 GO:0070011 GO:0070013 GO:0070727 GO:0070887 GO:0071310 GO:0071375 GO:0071417 GO:0071495 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072662 GO:0072663 GO:0080090 GO:0097159 GO:0097242 GO:0097367 GO:0140030 GO:0140035 GO:0140036 GO:0140096 GO:1901142 GO:1901143 GO:1901265 GO:1901363 GO:1901564 GO:1901565 GO:1901575 GO:1901652 GO:1901653 GO:1901698 GO:1901699 GO:1901700 GO:1901701
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_M16 Peptidase_M16_C Peptidase_M16_M

Protein Analysis

318

Amino Acids

36.85

Weight (kDa)

8.58

Isoelectric Point (pI)

49.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M16_M PF16187 2 - 71 3.7e-11 Middle or third domain of peptidase_M16
Peptidase_M16_C PF05193 83 - 247 3.2e-09 Peptidase M16 inactive domain
PqqF-like_C_4 PF22456 183 - 248 4.5e-13 PQQ synthase PqqF-like, C-terminal lobe domain 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 722
AccBSI CCGCTC 1 cut(s) 502
AccI GTMKAC 1 cut(s) 117
AciI CCGC 1 cut(s) 502
AclWI GGATC 2 cut(s) 521, 811
AcsI RAATTY 3 cut(s) 322, 370, 484
AfaI GTAC 3 cut(s) 299, 381, 613
AgsI TTSAA 7 cut(s) 127, 305, 398, 494, 553, 580, 945
Alw21I GWGCWC 1 cut(s) 934
Alw26I GTCTC 1 cut(s) 771
AlwI GGATC 2 cut(s) 521, 811
Ama87I CYCGRG 1 cut(s) 927
AoxI GGCC 1 cut(s) 625
ApeKI GCWGC 2 cut(s) 62, 601
ApoI RAATTY 3 cut(s) 322, 370, 484
AspS9I GGNCC 3 cut(s) 288, 626, 790
AvaI CYCGRG 1 cut(s) 927
AvaII GGWCC 2 cut(s) 288, 790
BaeI ACNNNNGTAYC 2 cut(s) 66, 99
BanII GRGCYC 1 cut(s) 934
BarI GAAGNNNNNNTAC 2 cut(s) 372, 404
BbsI GAAGAC 1 cut(s) 419
Bbv12I GWGCWC 1 cut(s) 934
BbvCI CCTCAGC 2 cut(s) 279, 678
BbvI GCAGC 2 cut(s) 49, 613
BccI CCATC 1 cut(s) 828
BceAI ACGGC 1 cut(s) 521
BcgI CGANNNNNNTGC 2 cut(s) 679, 713
BcoDI GTCTC 1 cut(s) 771
BfaI CTAG 3 cut(s) 57, 525, 911
BglI GCCNNNNNGGC 1 cut(s) 53
BglII AGATCT 1 cut(s) 544
BisI GCNGC 2 cut(s) 63, 602
BlsI GCNGC 2 cut(s) 64, 603
Bme18I GGWCC 2 cut(s) 288, 790
BmeT110I CYCGRG 1 cut(s) 927
BmgT120I GGNCC 3 cut(s) 288, 626, 790
BmiI GGNNCC 1 cut(s) 570
BmsI GCATC 1 cut(s) 393
BpiI GAAGAC 1 cut(s) 419
BpmI CTGGAG 2 cut(s) 125, 752
Bpu10I CCTNAGC 4 cut(s) 110, 279, 587, 678
Bsa29I ATCGAT 1 cut(s) 843
BsaJI CCNNGG 2 cut(s) 291, 629
BsaXI ACNNNNNCTCC 2 cut(s) 725, 755
Bse1I ACTGG 2 cut(s) 735, 761
BseCI ATCGAT 1 cut(s) 843
BseDI CCNNGG 2 cut(s) 291, 629
BseGI GGATG 2 cut(s) 765, 820
BseMII CTCAG 2 cut(s) 293, 669
BseNI ACTGG 2 cut(s) 735, 761
BseRI GAGGAG 1 cut(s) 749
BseXI GCAGC 2 cut(s) 49, 613
BshFI GGCC 1 cut(s) 627
BshVI ATCGAT 1 cut(s) 843
BsiHKAI GWGCWC 1 cut(s) 934
BsiHKCI CYCGRG 1 cut(s) 927
BslFI GGGAC 1 cut(s) 505
BsmAI GTCTC 1 cut(s) 771
BsmFI GGGAC 1 cut(s) 505
BsnI GGCC 1 cut(s) 627
BsoBI CYCGRG 1 cut(s) 927
Bsp1286I GDGCHC 1 cut(s) 934
Bsp1407I TGTACA 2 cut(s) 297, 379
Bsp143I GATC 5 cut(s) 193, 513, 544, 690, 816
BspACI CCGC 1 cut(s) 502
BspANI GGCC 1 cut(s) 627
BspCNI CTCAG 2 cut(s) 292, 670
BspDI ATCGAT 1 cut(s) 843
BspLI GGNNCC 1 cut(s) 570
BspPI GGATC 2 cut(s) 521, 811
BsrBI CCGCTC 1 cut(s) 502
BsrGI TGTACA 2 cut(s) 297, 379
BsrI ACTGG 2 cut(s) 735, 761
BssECI CCNNGG 2 cut(s) 291, 629
BssMI GATC 5 cut(s) 193, 513, 544, 690, 816
BssT1I CCWWGG 1 cut(s) 291
Bst4CI ACNGT 3 cut(s) 122, 417, 616
BstAUI TGTACA 2 cut(s) 297, 379
BstC8I GCNNGC 1 cut(s) 895
BstDEI CTNAG 4 cut(s) 110, 279, 587, 678
BstDSI CCRYGG 1 cut(s) 629
BstF5I GGATG 2 cut(s) 765, 820
BstKTI GATC 5 cut(s) 196, 516, 547, 693, 819
BstMAI GTCTC 1 cut(s) 771
BstMBI GATC 5 cut(s) 193, 513, 544, 690, 816
BstMWI GCNNNNNNNGC 2 cut(s) 53, 62
BstV1I GCAGC 2 cut(s) 49, 613
BstV2I GAAGAC 1 cut(s) 419
BstX2I RGATCY 2 cut(s) 544, 816
BstYI RGATCY 2 cut(s) 544, 816
Bsu15I ATCGAT 1 cut(s) 843
BsuRI GGCC 1 cut(s) 627
BsuTUI ATCGAT 1 cut(s) 843
BtgI CCRYGG 1 cut(s) 629
BtsCI GGATG 2 cut(s) 765, 820
BtsIMutI CAGTG 2 cut(s) 422, 466
Cac8I GCNNGC 1 cut(s) 895
Cfr13I GGNCC 3 cut(s) 288, 626, 790
ClaI ATCGAT 1 cut(s) 843
Csp6I GTAC 3 cut(s) 298, 380, 612
CviAII CATG 2 cut(s) 181, 317
CviQI GTAC 3 cut(s) 298, 380, 612
DdeI CTNAG 4 cut(s) 110, 279, 587, 678
DpnI GATC 5 cut(s) 195, 515, 546, 692, 818
DpnII GATC 5 cut(s) 193, 513, 544, 690, 816
DraI TTTAAA 1 cut(s) 364
Ecl136II GAGCTC 1 cut(s) 932
Eco130I CCWWGG 1 cut(s) 291
Eco24I GRGCYC 1 cut(s) 934
Eco32I GATATC 1 cut(s) 457
Eco47I GGWCC 2 cut(s) 288, 790
Eco53kI GAGCTC 1 cut(s) 932
Eco88I CYCGRG 1 cut(s) 927
EcoICRI GAGCTC 1 cut(s) 932
EcoRV GATATC 1 cut(s) 457
EcoT14I CCWWGG 1 cut(s) 291
EcoT38I GRGCYC 1 cut(s) 934
ErhI CCWWGG 1 cut(s) 291
FaeI CATG 2 cut(s) 184, 320
FaqI GGGAC 1 cut(s) 505
FatI CATG 2 cut(s) 180, 316
FauI CCCGC 1 cut(s) 495
FblI GTMKAC 1 cut(s) 117
Fnu4HI GCNGC 2 cut(s) 63, 602
FokI GGATG 2 cut(s) 772, 807
FriOI GRGCYC 1 cut(s) 934
Fsp4HI GCNGC 2 cut(s) 63, 602
FspBI CTAG 3 cut(s) 57, 525, 911
GluI GCNGC 2 cut(s) 63, 602
GsuI CTGGAG 2 cut(s) 125, 752
HaeIII GGCC 1 cut(s) 627
Hin1II CATG 2 cut(s) 184, 320
HindIII AAGCTT 2 cut(s) 134, 902
HinfI GANTC 2 cut(s) 157, 518
Hpy166II GTNNAC 5 cut(s) 118, 245, 298, 380, 413
Hpy188I TCNGA 6 cut(s) 162, 193, 259, 331, 355, 936
Hpy188III TCNNGA 1 cut(s) 71
Hpy8I GTNNAC 5 cut(s) 118, 245, 298, 380, 413
HpyAV CCTTC 2 cut(s) 444, 589
HpyCH4III ACNGT 3 cut(s) 122, 417, 616
HpyCH4V TGCA 4 cut(s) 232, 642, 700, 799
HpyF10VI GCNNNNNNNGC 2 cut(s) 53, 62
HpyF3I CTNAG 4 cut(s) 110, 279, 587, 678
Hsp92II CATG 2 cut(s) 184, 320
Kzo9I GATC 5 cut(s) 193, 513, 544, 690, 816
LmnI GCTCC 2 cut(s) 106, 598
Lsp1109I GCAGC 2 cut(s) 49, 613
LweI GCATC 1 cut(s) 393
MaeI CTAG 3 cut(s) 57, 525, 911
MaeIII GTNAC 2 cut(s) 634, 851
MalI GATC 5 cut(s) 195, 515, 546, 692, 818
MbiI CCGCTC 1 cut(s) 502
MboI GATC 5 cut(s) 193, 513, 544, 690, 816
MboII GAAGA 2 cut(s) 419, 478
MfeI CAATTG 2 cut(s) 393, 794
MflI RGATCY 2 cut(s) 544, 816
MhlI GDGCHC 1 cut(s) 934
MlyI GAGTC 1 cut(s) 527
MseI TTAA 2 cut(s) 239, 363
MspA1I CMGCKG 1 cut(s) 677
MunI CAATTG 2 cut(s) 393, 794
MwoI GCNNNNNNNGC 2 cut(s) 53, 62
NdeII GATC 5 cut(s) 193, 513, 544, 690, 816
NlaIII CATG 2 cut(s) 184, 320
NlaIV GGNNCC 1 cut(s) 570
PaeR7I CTCGAG 1 cut(s) 927
PfeI GAWTC 1 cut(s) 157
PkrI GCNGC 2 cut(s) 64, 603
PleI GAGTC 1 cut(s) 526
PpsI GAGTC 1 cut(s) 526
PsiI TTATAA 1 cut(s) 722
Psp124BI GAGCTC 1 cut(s) 934
PspN4I GGNNCC 1 cut(s) 570
PspPI GGNCC 3 cut(s) 288, 626, 790
PspXI VCTCGAGB 1 cut(s) 927
PsuI RGATCY 2 cut(s) 544, 816
PvuII CAGCTG 1 cut(s) 677
RsaI GTAC 3 cut(s) 299, 381, 613
RsaNI GTAC 3 cut(s) 298, 380, 612
SacI GAGCTC 1 cut(s) 934
SaqAI TTAA 2 cut(s) 239, 363
SatI GCNGC 2 cut(s) 63, 602
Sau3AI GATC 5 cut(s) 193, 513, 544, 690, 816
Sau96I GGNCC 3 cut(s) 288, 626, 790
SchI GAGTC 1 cut(s) 527
SduI GDGCHC 1 cut(s) 934
SfaNI GCATC 1 cut(s) 393
Sfr274I CTCGAG 1 cut(s) 927
SinI GGWCC 2 cut(s) 288, 790
SlaI CTCGAG 1 cut(s) 927
SmlI CTYRAG 1 cut(s) 927
SmoI CTYRAG 1 cut(s) 927
SsiI CCGC 1 cut(s) 502
SspMI CTAG 3 cut(s) 57, 525, 911
SstI GAGCTC 1 cut(s) 934
StyI CCWWGG 1 cut(s) 291
TaaI ACNGT 3 cut(s) 122, 417, 616
TaqI TCGA 4 cut(s) 92, 516, 843, 928
TatI WGTACW 3 cut(s) 297, 379, 611
TfiI GAWTC 1 cut(s) 157
Tru1I TTAA 2 cut(s) 239, 363
Tru9I TTAA 2 cut(s) 239, 363
TscAI CASTG 2 cut(s) 422, 466
TseI GCWGC 2 cut(s) 62, 601
TspDTI ATGAA 4 cut(s) 169, 570, 608, 848
TspRI CASTG 2 cut(s) 422, 466
VpaK11BI GGWCC 2 cut(s) 288, 790
XapI RAATTY 3 cut(s) 322, 370, 484
XhoI CTCGAG 1 cut(s) 927
XmiI GTMKAC 1 cut(s) 117
XspI CTAG 3 cut(s) 57, 525, 911
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.