RLG00000030375

Belongs to the peptidase M16 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0000891
Physical Location & Seq
Reverse (-)
60287169 .. 60309612
22444 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030375

Sequence Viewer

Length: 1392 bp
ATGATGGAGAAGACGAAGATAGTGAAAGAGGGGATGAAGAAGAAAAAGGGAGGGGATTCTCAGACTAACAAGGTAGCAGCAGCAATGTGTGTACGAGTAGGCAACTTCTCTGACCCTCTTGAGGCACAGGGGCTTGCACACTTTCTAGAACACATGCTCTTTATGTGGAAATCGCTTGATGTACATGAGAATTGGGTTTTGGAATTGTTTGGTAACGTCAAAAAAGGTCCCCAAGTAAATCTGGAATTCAAGGCATGCAGAAGGTCCAATTTGGAAAGCTGGAAAACTTTACAGGCTAGAGGCTGGAAAGTTTGCATTCCTATTTCAAAGTTAAAGGATAGGCAACATCTCTGGCCGGTTGCTGGTGTTGGGGATGACGGGATGCATCATTCTTTTGTGGCTTATGTCTTTCGCATGGACATTCATCTCACTGACTATGGATTGGATAAGAATTGCAGGATACTGGGAGCATGGAATTTAGATTTGCAGAGGAGCAGCCTCAGGATGGTCATGCCTCAGAACTTGCAGGTTTCTTTTTATTTGTTTAAACTGTTTCATATTTCCGATGCGAACCTTTGGTTTGGGTCACATTATACTGAGGAAGATATATCTCCATCTTTGATAGATTTGTGGAAGGACCCTCCAGAAATTGATGTTTCATTGCATCTCCCAGAAAAGAATGAGTTCATTCCTACTGATTTTTCCATTTTTTCTGATGTGTTGCCAAGCTGGAAACTTCGGTATTTGTATCAACTGACAAGCTGGAAGGTCTACGGTTTTAATGACAAGCTTCCAGCTCTGTTGTCAAAGATTCTGAAAACAACCAAAAGTTTCATGCCAACTTCTGATCGTTTTACGTTAAAGAACACCAATATGAAGCCTCTGAGTCACTCTACATACTTGAGAGTGCAAGTTTTGTTCCAGAGATTCTATGATGTTGATGAGAAGTTGCATGTTTTAAGTGGACTGTCTATTTGTGATTCTAGCATTGTTTCCAGCCTCTGTCATGGAAATTTGTCAGAAAAAGAAGCAATCAGCCTTTCAGATATATTTAAAGCAAATTTTGGTGTACAACCACTTCCAATTGAACTGATGCATAGTGAACAGTGTATTTGTCTTCCTCCTGCTAGCCTTATTAGAGATGTAAGTGTGAAGGATAAATCTGAAACAAATTCAGAAGCTAGATCTTGCAACTTGAGGTGCCATAATGAAATGTACAAGGCATTTCTTGATCATGGGAATCTGATTTTATTCTATTATGCTCGCCAAGGACTGGACGATGACTGTTTGGAGAATTACAGAGCTAGGCTAATTGCAAAGCTATTGGAGAAAGATCCACCCCTCCATTATGAAACCAATCGATATCGGTCACAGATTACCGATAAAATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0003674 GO:0003824 GO:0004175 GO:0004222 GO:0005102 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005777 GO:0005782 GO:0005829 GO:0006508 GO:0006518 GO:0006605 GO:0006625 GO:0006807 GO:0006810 GO:0006886 GO:0006996 GO:0007031 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007169 GO:0007275 GO:0007568 GO:0008104 GO:0008144 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0008270 GO:0008286 GO:0008340 GO:0009056 GO:0009057 GO:0009719 GO:0009725 GO:0009893 GO:0009894 GO:0009896 GO:0009986 GO:0009987 GO:0010033 GO:0010243 GO:0010259 GO:0010604 GO:0010815 GO:0010992 GO:0015031 GO:0015833 GO:0016043 GO:0016787 GO:0017046 GO:0017076 GO:0017144 GO:0019222 GO:0019538 GO:0019725 GO:0022607 GO:0023052 GO:0030163 GO:0030554 GO:0031334 GO:0031907 GO:0031974 GO:0032459 GO:0032461 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0032868 GO:0032869 GO:0032870 GO:0033036 GO:0033218 GO:0033365 GO:0034613 GO:0034641 GO:0035639 GO:0036094 GO:0042176 GO:0042221 GO:0042277 GO:0042562 GO:0042579 GO:0042592 GO:0042737 GO:0042802 GO:0042803 GO:0042886 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043171 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043254 GO:0043434 GO:0043559 GO:0043574 GO:0043603 GO:0043933 GO:0044085 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044421 GO:0044422 GO:0044424 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0045732 GO:0046872 GO:0046907 GO:0046914 GO:0046983 GO:0048518 GO:0048522 GO:0048856 GO:0050435 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051259 GO:0051260 GO:0051603 GO:0051641 GO:0051649 GO:0051716 GO:0060255 GO:0065003 GO:0065007 GO:0065008 GO:0070011 GO:0070013 GO:0070727 GO:0070887 GO:0071310 GO:0071375 GO:0071417 GO:0071495 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072662 GO:0072663 GO:0080090 GO:0097159 GO:0097242 GO:0097367 GO:0140030 GO:0140035 GO:0140036 GO:0140096 GO:1901142 GO:1901143 GO:1901265 GO:1901363 GO:1901564 GO:1901565 GO:1901575 GO:1901652 GO:1901653 GO:1901698 GO:1901699 GO:1901700 GO:1901701
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_M16 Peptidase_M16_C Peptidase_M16_M

Protein Analysis

464

Amino Acids

53.5

Weight (kDa)

8.17

Isoelectric Point (pI)

43.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M16 PF00675 20 - 55 6e-07 Insulinase (Peptidase family M16)
Peptidase_M16_M PF16187 157 - 238 1.3e-12 Middle or third domain of peptidase_M16
Peptidase_M16_M PF16187 256 - 318 1.5e-06 Middle or third domain of peptidase_M16
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 517
AccB1I GGYRCC 1 cut(s) 1200
AccB7I CCANNNNNTGG 1 cut(s) 1273
AccI GTMKAC 1 cut(s) 771
AclWI GGATC 1 cut(s) 1328
AcoI YGGCCR 1 cut(s) 353
AcsI RAATTY 5 cut(s) 245, 475, 1012, 1060, 1171
AfaI GTAC 4 cut(s) 93, 183, 1071, 1217
AfiI CCNNNNNNNGG 4 cut(s) 121, 362, 505, 1273
AgsI TTSAA 3 cut(s) 250, 327, 1088
AjuI GAANNNNNNNTTGG 2 cut(s) 182, 214
AluBI AGCT 8 cut(s) 279, 729, 762, 790, 797, 1181, 1304, 1321
AluI AGCT 8 cut(s) 279, 729, 762, 790, 797, 1181, 1304, 1321
AlwI GGATC 1 cut(s) 1328
AlwNI CAGNNNCTG 1 cut(s) 1002
AoxI GGCC 1 cut(s) 353
ApeKI GCWGC 3 cut(s) 77, 80, 495
ApoI RAATTY 5 cut(s) 245, 475, 1012, 1060, 1171
ArsI GACNNNNNNTTYG 2 cut(s) 957, 989
Asp700I GAANNNNTTC 1 cut(s) 683
AspS9I GGNCC 3 cut(s) 227, 264, 637
AsuNHI GCTAGC 1 cut(s) 1127
AvaII GGWCC 3 cut(s) 227, 264, 637
AxyI CCTNAGG 1 cut(s) 500
BanI GGYRCC 1 cut(s) 1200
BarI GAAGNNNNNNTAC 2 cut(s) 1062, 1094
BbsI GAAGAC 2 cut(s) 17, 1109
BbvI GCAGC 3 cut(s) 89, 92, 507
BccI CCATC 2 cut(s) 499, 622
BciVI GTATCC 1 cut(s) 453
BclI TGATCA 1 cut(s) 1231
BfaI CTAG 6 cut(s) 146, 297, 984, 1128, 1182, 1305
BfuAI ACCTGC 1 cut(s) 517
BfuI GTATCC 1 cut(s) 453
BglII AGATCT 1 cut(s) 1184
BisI GCNGC 3 cut(s) 78, 81, 496
BlsI GCNGC 3 cut(s) 79, 82, 497
Bme18I GGWCC 3 cut(s) 227, 264, 637
BmgT120I GGNCC 3 cut(s) 227, 264, 637
BmiI GGNNCC 3 cut(s) 229, 639, 1202
BmrI ACTGGG 1 cut(s) 473
BmsI GCATC 5 cut(s) 372, 394, 556, 673, 1083
BmtI GCTAGC 1 cut(s) 1131
BmuI ACTGGG 1 cut(s) 473
BpiI GAAGAC 2 cut(s) 17, 1109
BpmI CTGGAG 1 cut(s) 627
BpuEI CTTGAG 3 cut(s) 140, 922, 1216
Bsa29I ATCGAT 1 cut(s) 1360
BsaJI CCNNGG 1 cut(s) 1267
Bsc4I CCNNNNNNNGG 4 cut(s) 121, 362, 505, 1273
Bse118I RCCGGY 1 cut(s) 355
Bse1I ACTGG 2 cut(s) 468, 1278
Bse21I CCTNAGG 1 cut(s) 500
Bse3DI GCAATG 2 cut(s) 90, 659
BseCI ATCGAT 1 cut(s) 1360
BseDI CCNNGG 1 cut(s) 1267
BseGI GGATG 4 cut(s) 39, 379, 387, 510
BseLI CCNNNNNNNGG 4 cut(s) 121, 362, 505, 1273
BseMI GCAATG 2 cut(s) 90, 659
BseMII CTCAG 5 cut(s) 74, 514, 530, 588, 875
BseNI ACTGG 2 cut(s) 468, 1278
BseRI GAGGAG 1 cut(s) 505
BseXI GCAGC 3 cut(s) 89, 92, 507
BshFI GGCC 1 cut(s) 355
BshNI GGYRCC 1 cut(s) 1200
BshVI ATCGAT 1 cut(s) 1360
BsiSI CCGG 1 cut(s) 356
BslFI GGGAC 1 cut(s) 213
BslI CCNNNNNNNGG 4 cut(s) 121, 362, 505, 1273
BsmFI GGGAC 1 cut(s) 213
BsmI GAATGC 1 cut(s) 315
BsnI GGCC 1 cut(s) 355
Bsp1407I TGTACA 3 cut(s) 181, 1069, 1215
Bsp143I GATC 4 cut(s) 847, 1184, 1231, 1333
BspANI GGCC 1 cut(s) 355
BspCNI CTCAG 5 cut(s) 73, 513, 529, 589, 876
BspDI ATCGAT 1 cut(s) 1360
BspLI GGNNCC 3 cut(s) 229, 639, 1202
BspMI ACCTGC 1 cut(s) 517
BspOI GCTAGC 1 cut(s) 1131
BspPI GGATC 1 cut(s) 1328
BspT107I GGYRCC 1 cut(s) 1200
BsrDI GCAATG 2 cut(s) 90, 659
BsrFI RCCGGY 1 cut(s) 355
BsrGI TGTACA 3 cut(s) 181, 1069, 1215
BsrI ACTGG 2 cut(s) 468, 1278
BssAI RCCGGY 1 cut(s) 355
BssECI CCNNGG 1 cut(s) 1267
BssMI GATC 4 cut(s) 847, 1184, 1231, 1333
BssT1I CCWWGG 1 cut(s) 1267
Bst4CI ACNGT 5 cut(s) 552, 776, 969, 1107, 1286
BstAUI TGTACA 3 cut(s) 181, 1069, 1215
BstC8I GCNNGC 4 cut(s) 135, 256, 1129, 1264
BstDEI CTNAG 5 cut(s) 60, 500, 516, 597, 884
BstF5I GGATG 4 cut(s) 39, 379, 387, 510
BstKTI GATC 4 cut(s) 850, 1187, 1234, 1336
BstMBI GATC 4 cut(s) 847, 1184, 1231, 1333
BstNSI RCATGY 3 cut(s) 157, 258, 956
BstV1I GCAGC 3 cut(s) 89, 92, 507
BstV2I GAAGAC 2 cut(s) 17, 1109
BstX2I RGATCY 2 cut(s) 1184, 1333
BstYI RGATCY 2 cut(s) 1184, 1333
Bsu15I ATCGAT 1 cut(s) 1360
Bsu36I CCTNAGG 1 cut(s) 500
BsuI GTATCC 1 cut(s) 453
BsuRI GGCC 1 cut(s) 355
BsuTUI ATCGAT 1 cut(s) 1360
BtsCI GGATG 4 cut(s) 39, 379, 387, 510
BtsIMutI CAGTG 2 cut(s) 429, 1112
BveI ACCTGC 1 cut(s) 517
Cac8I GCNNGC 4 cut(s) 135, 256, 1129, 1264
CaiI CAGNNNCTG 1 cut(s) 1002
Cfr10I RCCGGY 1 cut(s) 355
Cfr13I GGNCC 3 cut(s) 227, 264, 637
ClaI ATCGAT 1 cut(s) 1360
Csp6I GTAC 4 cut(s) 92, 182, 1070, 1216
CviQI GTAC 4 cut(s) 92, 182, 1070, 1216
DdeI CTNAG 5 cut(s) 60, 500, 516, 597, 884
DpnI GATC 4 cut(s) 849, 1186, 1233, 1335
DpnII GATC 4 cut(s) 847, 1184, 1231, 1333
DraI TTTAAA 2 cut(s) 547, 1054
EaeI YGGCCR 1 cut(s) 353
Eco130I CCWWGG 1 cut(s) 1267
Eco32I GATATC 1 cut(s) 1364
Eco47I GGWCC 3 cut(s) 227, 264, 637
Eco81I CCTNAGG 1 cut(s) 500
EcoO109I RGGNCCY 2 cut(s) 227, 637
EcoRI GAATTC 1 cut(s) 245
EcoRV GATATC 1 cut(s) 1364
EcoT14I CCWWGG 1 cut(s) 1267
EcoT22I ATGCAT 2 cut(s) 387, 1098
ErhI CCWWGG 1 cut(s) 1267
FaqI GGGAC 1 cut(s) 213
FbaI TGATCA 1 cut(s) 1231
FblI GTMKAC 1 cut(s) 771
Fnu4HI GCNGC 3 cut(s) 78, 81, 496
FokI GGATG 4 cut(s) 46, 386, 394, 517
Fsp4HI GCNGC 3 cut(s) 78, 81, 496
FspBI CTAG 6 cut(s) 146, 297, 984, 1128, 1182, 1305
GluI GCNGC 3 cut(s) 78, 81, 496
GsuI CTGGAG 1 cut(s) 627
HaeIII GGCC 1 cut(s) 355
HapII CCGG 1 cut(s) 356
HindIII AAGCTT 1 cut(s) 788
HinfI GANTC 6 cut(s) 56, 811, 886, 927, 980, 1240
HpaII CCGG 1 cut(s) 356
Hpy166II GTNNAC 5 cut(s) 92, 772, 965, 1070, 1103
Hpy188III TCNNGA 7 cut(s) 119, 146, 242, 502, 644, 922, 1229
Hpy8I GTNNAC 5 cut(s) 92, 772, 965, 1070, 1103
HpyAV CCTTC 4 cut(s) 255, 628, 760, 1147
HpyCH4III ACNGT 5 cut(s) 552, 776, 969, 1107, 1286
HpyCH4IV ACGT 2 cut(s) 216, 857
HpyF3I CTNAG 5 cut(s) 60, 500, 516, 597, 884
HpySE526I ACGT 2 cut(s) 216, 857
Ksp22I TGATCA 1 cut(s) 1231
Kzo9I GATC 4 cut(s) 847, 1184, 1231, 1333
LmnI GCTCC 2 cut(s) 467, 492
Lsp1109I GCAGC 3 cut(s) 89, 92, 507
LweI GCATC 5 cut(s) 372, 394, 556, 673, 1083
MaeI CTAG 6 cut(s) 146, 297, 984, 1128, 1182, 1305
MaeII ACGT 2 cut(s) 216, 857
MaeIII GTNAC 4 cut(s) 212, 585, 887, 1368
MalI GATC 4 cut(s) 849, 1186, 1233, 1335
MboI GATC 4 cut(s) 847, 1184, 1231, 1333
MboII GAAGA 6 cut(s) 22, 28, 49, 52, 614, 1109
MfeI CAATTG 1 cut(s) 1083
MflI RGATCY 2 cut(s) 1184, 1333
MlyI GAGTC 1 cut(s) 895
Mph1103I ATGCAT 2 cut(s) 387, 1098
MroXI GAANNNNTTC 1 cut(s) 683
MseI TTAA 6 cut(s) 332, 546, 780, 860, 959, 1053
MslI CAYNNNNRTG 1 cut(s) 872
MspI CCGG 1 cut(s) 356
MssI GTTTAAAC 1 cut(s) 547
MunI CAATTG 1 cut(s) 1083
Mva1269I GAATGC 1 cut(s) 315
NdeII GATC 4 cut(s) 847, 1184, 1231, 1333
NheI GCTAGC 1 cut(s) 1127
NlaIV GGNNCC 3 cut(s) 229, 639, 1202
NmuCI GTSAC 3 cut(s) 585, 887, 1368
NsiI ATGCAT 2 cut(s) 387, 1098
NspI RCATGY 3 cut(s) 157, 258, 956
PaeI GCATGC 1 cut(s) 258
PctI GAATGC 1 cut(s) 315
PdmI GAANNNNTTC 1 cut(s) 683
PfeI GAWTC 5 cut(s) 56, 811, 927, 980, 1240
PflMI CCANNNNNTGG 1 cut(s) 1273
PkrI GCNGC 3 cut(s) 79, 82, 497
PleI GAGTC 1 cut(s) 894
PmeI GTTTAAAC 1 cut(s) 547
PpsI GAGTC 1 cut(s) 894
PpuMI RGGWCCY 2 cut(s) 227, 637
Psp5II RGGWCCY 2 cut(s) 227, 637
PspN4I GGNNCC 3 cut(s) 229, 639, 1202
PspPI GGNCC 3 cut(s) 227, 264, 637
PspPPI RGGWCCY 2 cut(s) 227, 637
PstNI CAGNNNCTG 1 cut(s) 1002
PsuI RGATCY 2 cut(s) 1184, 1333
RsaI GTAC 4 cut(s) 93, 183, 1071, 1217
RsaNI GTAC 4 cut(s) 92, 182, 1070, 1216
RseI CAYNNNNRTG 1 cut(s) 872
SaqAI TTAA 6 cut(s) 332, 546, 780, 860, 959, 1053
SatI GCNGC 3 cut(s) 78, 81, 496
Sau3AI GATC 4 cut(s) 847, 1184, 1231, 1333
Sau96I GGNCC 3 cut(s) 227, 264, 637
SchI GAGTC 1 cut(s) 895
SfaNI GCATC 5 cut(s) 372, 394, 556, 673, 1083
SinI GGWCC 3 cut(s) 227, 264, 637
SmiMI CAYNNNNRTG 1 cut(s) 872
SmlI CTYRAG 3 cut(s) 119, 901, 1195
SmoI CTYRAG 3 cut(s) 119, 901, 1195
SphI GCATGC 1 cut(s) 258
SspMI CTAG 6 cut(s) 146, 297, 984, 1128, 1182, 1305
StyI CCWWGG 1 cut(s) 1267
TaaI ACNGT 5 cut(s) 552, 776, 969, 1107, 1286
TaiI ACGT 2 cut(s) 219, 860
TaqI TCGA 1 cut(s) 1360
TaqII GACCGA 1 cut(s) 1356
TatI WGTACW 3 cut(s) 181, 1069, 1215
TfiI GAWTC 5 cut(s) 56, 811, 927, 980, 1240
Tru1I TTAA 6 cut(s) 332, 546, 780, 860, 959, 1053
Tru9I TTAA 6 cut(s) 332, 546, 780, 860, 959, 1053
TscAI CASTG 2 cut(s) 436, 1112
TseFI GTSAC 3 cut(s) 585, 887, 1368
TseI GCWGC 3 cut(s) 77, 80, 495
Tsp45I GTSAC 3 cut(s) 585, 887, 1368
TspDTI ATGAA 9 cut(s) 50, 413, 545, 648, 676, 823, 890, 1224, 1365
TspRI CASTG 2 cut(s) 436, 1112
Van91I CCANNNNNTGG 1 cut(s) 1273
VpaK11BI GGWCC 3 cut(s) 227, 264, 637
XapI RAATTY 5 cut(s) 245, 475, 1012, 1060, 1171
XbaI TCTAGA 1 cut(s) 145
XceI RCATGY 3 cut(s) 157, 258, 956
XmiI GTMKAC 1 cut(s) 771
XmnI GAANNNNTTC 1 cut(s) 683
XspI CTAG 6 cut(s) 146, 297, 984, 1128, 1182, 1305
Zsp2I ATGCAT 2 cut(s) 387, 1098
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.