RchiOBHm_Chr1g0333061

Belongs to the peptidase M16 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000891
Physical Location & Seq
Forward (+)
25004308 .. 25008396
4089 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ56190

Sequence Viewer

Length: 837 bp
ATGTGGGTAGGAATAGGCAGCTTCTCTGACCCTCCTGAGCCACAGGGGCTTGCACACTTTCTAGAACACATGCTTTTCATGGGGAGTACGGAATTTCCAGATGAAAATGAGCACGGAGGGTGGTCAAATCCATATGCAGAAGTAGAGCATACTTGCTACCATTTTGAAGTGAAACAAGAGTTTCTCAAGGGTGCCTTGACAAGGATCTGTGGTCTCTTTGTTTCACCCCTTGTAAAAAATGAAGCCATGGAGCGGGAGGTACAGGCTGTAGATTCAGAGTTTAACCAGGTTCTGCAGAACGGTGCTTGCCACCTTGAACAACTTCAATGCCATACAGCCTCAACTGAATCTCTTGATGTAATTGAGAATTGGGTTTTGGAATTGTTTGGTAAAGTCAAAGAAGGTCCCCCAGTAAATCTGGAATTCAAGGCTGAAGGTCCAATTTGGAAAGCTGGAAAACTTTACAGGCTAGGGGCTGTTAAAGATGTTCGTATACTCCACTTAACATGGACATTGCCATGCCCTCGTCAAGACTATTTGAAGAAATCAGAAGAATATTTATCTCATCTCCTTGGGCATGAGGGCAGGGGAAGTTTGCATTCCTATTTCAAAGTTAAAGGGTGGGCAACATCTCTGGCTGCTGGTGTTGGGGATGGCGGGATGCATCATTTTTTTTTTGGTTTATGTCTTTCGCACGGACATTCATCTCACTGGCCAGTCTCTGGATTGGATAAGATTTTTTATATAATTGGCTTGGTCTACCAATACATAAATTTATTGCATCAAGTGTCCCCACAACAATGGATATTTAAGGAACTGCAGGATACTGGGATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0003674 GO:0003824 GO:0004175 GO:0004222 GO:0005102 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005777 GO:0005782 GO:0005829 GO:0006508 GO:0006518 GO:0006605 GO:0006625 GO:0006807 GO:0006810 GO:0006886 GO:0006996 GO:0007031 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007169 GO:0007275 GO:0007568 GO:0008104 GO:0008144 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0008270 GO:0008286 GO:0008340 GO:0009056 GO:0009057 GO:0009719 GO:0009725 GO:0009893 GO:0009894 GO:0009896 GO:0009986 GO:0009987 GO:0010033 GO:0010243 GO:0010259 GO:0010604 GO:0010815 GO:0010992 GO:0015031 GO:0015833 GO:0016043 GO:0016787 GO:0017046 GO:0017076 GO:0017144 GO:0019222 GO:0019538 GO:0019725 GO:0022607 GO:0023052 GO:0030163 GO:0030554 GO:0031334 GO:0031907 GO:0031974 GO:0032459 GO:0032461 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0032868 GO:0032869 GO:0032870 GO:0033036 GO:0033218 GO:0033365 GO:0034613 GO:0034641 GO:0035639 GO:0036094 GO:0042176 GO:0042221 GO:0042277 GO:0042562 GO:0042579 GO:0042592 GO:0042737 GO:0042802 GO:0042803 GO:0042886 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043171 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043254 GO:0043434 GO:0043559 GO:0043574 GO:0043603 GO:0043933 GO:0044085 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044421 GO:0044422 GO:0044424 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0045732 GO:0046872 GO:0046907 GO:0046914 GO:0046983 GO:0048518 GO:0048522 GO:0048856 GO:0050435 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051259 GO:0051260 GO:0051603 GO:0051641 GO:0051649 GO:0051716 GO:0060255 GO:0065003 GO:0065007 GO:0065008 GO:0070011 GO:0070013 GO:0070727 GO:0070887 GO:0071310 GO:0071375 GO:0071417 GO:0071495 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072662 GO:0072663 GO:0080090 GO:0097159 GO:0097242 GO:0097367 GO:0140030 GO:0140035 GO:0140036 GO:0140096 GO:1901142 GO:1901143 GO:1901265 GO:1901363 GO:1901564 GO:1901565 GO:1901575 GO:1901652 GO:1901653 GO:1901698 GO:1901699 GO:1901700 GO:1901701
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_M16 Peptidase_M16_C Peptidase_M16_M

Protein Analysis

278

Amino Acids

31.46

Weight (kDa)

5.75

Isoelectric Point (pI)

29.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M16 PF00675 1 - 101 2.8e-20 Insulinase (Peptidase family M16)
Peptidase_M16_C PF05193 116 - 227 3.3e-07 Peptidase M16 inactive domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 191
AccB7I CCANNNNNTGG 1 cut(s) 722
AccBSI CCGCTC 1 cut(s) 253
AccI GTMKAC 2 cut(s) 493, 759
AciI CCGC 2 cut(s) 253, 657
AclWI GGATC 1 cut(s) 212
AcoI YGGCCR 1 cut(s) 713
AcsI RAATTY 3 cut(s) 92, 422, 772
AcuI CTGAAG 1 cut(s) 453
AfaI GTAC 2 cut(s) 88, 261
AfiI CCNNNNNNNGG 3 cut(s) 201, 252, 722
AgsI TTSAA 6 cut(s) 167, 317, 326, 427, 541, 610
AjnI CCWGG 1 cut(s) 285
AjuI GAANNNNNNNTTGG 2 cut(s) 359, 391
AluBI AGCT 2 cut(s) 21, 452
AluI AGCT 2 cut(s) 21, 452
Alw21I GWGCWC 1 cut(s) 114
Alw26I GTCTC 2 cut(s) 218, 724
AlwI GGATC 1 cut(s) 212
AlwNI CAGNNNCTG 2 cut(s) 292, 722
AoxI GGCC 1 cut(s) 713
ApeKI GCWGC 2 cut(s) 18, 638
ApoI RAATTY 3 cut(s) 92, 422, 772
Asp700I GAANNNNTTC 1 cut(s) 321
AspS9I GGNCC 2 cut(s) 404, 437
AsuHPI GGTGA 1 cut(s) 216
AvaII GGWCC 2 cut(s) 404, 437
BalI TGGCCA 1 cut(s) 715
BanI GGYRCC 1 cut(s) 191
Bbv12I GWGCWC 1 cut(s) 114
BbvI GCAGC 2 cut(s) 30, 625
BccI CCATC 1 cut(s) 647
BciT130I CCWGG 1 cut(s) 287
BciVI GTATCC 1 cut(s) 817
BcoDI GTCTC 2 cut(s) 218, 724
BfaI CTAG 2 cut(s) 62, 470
BfmI CTRYAG 3 cut(s) 267, 293, 818
BfuI GTATCC 1 cut(s) 817
BglI GCCNNNNNGGC 1 cut(s) 46
BisI GCNGC 2 cut(s) 19, 639
BlsI GCNGC 2 cut(s) 20, 640
Bme1390I CCNGG 1 cut(s) 287
Bme18I GGWCC 2 cut(s) 404, 437
BmgT120I GGNCC 2 cut(s) 404, 437
BmiI GGNNCC 2 cut(s) 193, 406
BmrFI CCNGG 1 cut(s) 287
BmrI ACTGGG 2 cut(s) 404, 837
BmsI GCATC 3 cut(s) 651, 673, 790
BmuI ACTGGG 2 cut(s) 404, 837
Bpu10I CCTNAGC 1 cut(s) 36
BpuEI CTTGAG 1 cut(s) 170
BsaI GGTCTC 1 cut(s) 218
BsaJI CCNNGG 2 cut(s) 246, 571
Bsc4I CCNNNNNNNGG 3 cut(s) 201, 252, 722
Bse1I ACTGG 4 cut(s) 410, 716, 716, 832
Bse3DI GCAATG 1 cut(s) 512
BseBI CCWGG 1 cut(s) 287
BseDI CCNNGG 2 cut(s) 246, 571
BseGI GGATG 2 cut(s) 658, 666
BseLI CCNNNNNNNGG 3 cut(s) 201, 252, 722
BseMI GCAATG 1 cut(s) 512
BseMII CTCAG 1 cut(s) 27
BseNI ACTGG 4 cut(s) 410, 716, 716, 832
BseXI GCAGC 2 cut(s) 30, 625
BshFI GGCC 1 cut(s) 715
BshNI GGYRCC 1 cut(s) 191
BsiHKAI GWGCWC 1 cut(s) 114
BslFI GGGAC 2 cut(s) 390, 775
BslI CCNNNNNNNGG 3 cut(s) 201, 252, 722
BsmAI GTCTC 2 cut(s) 218, 724
BsmFI GGGAC 2 cut(s) 390, 775
BsmI GAATGC 1 cut(s) 598
BsnI GGCC 1 cut(s) 715
Bso31I GGTCTC 1 cut(s) 218
Bsp1286I GDGCHC 1 cut(s) 114
Bsp143I GATC 1 cut(s) 204
Bsp19I CCATGG 1 cut(s) 246
BspACI CCGC 2 cut(s) 253, 657
BspANI GGCC 1 cut(s) 715
BspCNI CTCAG 1 cut(s) 28
BspLI GGNNCC 2 cut(s) 193, 406
BspMAI CTGCAG 2 cut(s) 297, 822
BspPI GGATC 1 cut(s) 212
BspT107I GGYRCC 1 cut(s) 191
BspTNI GGTCTC 1 cut(s) 218
BsrBI CCGCTC 1 cut(s) 253
BsrDI GCAATG 1 cut(s) 512
BsrI ACTGG 4 cut(s) 410, 716, 716, 832
BssECI CCNNGG 2 cut(s) 246, 571
BssMI GATC 1 cut(s) 204
BssNAI GTATAC 1 cut(s) 494
BssT1I CCWWGG 2 cut(s) 246, 571
Bst1107I GTATAC 1 cut(s) 494
Bst2UI CCWGG 1 cut(s) 287
Bst4CI ACNGT 1 cut(s) 302
BstC8I GCNNGC 2 cut(s) 51, 307
BstDEI CTNAG 1 cut(s) 36
BstDSI CCRYGG 1 cut(s) 246
BstENI CCTNNNNNAGG 1 cut(s) 199
BstF5I GGATG 2 cut(s) 658, 666
BstKTI GATC 1 cut(s) 207
BstMAI GTCTC 2 cut(s) 218, 724
BstMBI GATC 1 cut(s) 204
BstMWI GCNNNNNNNGC 1 cut(s) 46
BstNI CCWGG 1 cut(s) 287
BstNSI RCATGY 1 cut(s) 73
BstSCI CCNGG 1 cut(s) 285
BstSFI CTRYAG 3 cut(s) 267, 293, 818
BstV1I GCAGC 2 cut(s) 30, 625
BstX2I RGATCY 1 cut(s) 204
BstXI CCANNNNNNTGG 1 cut(s) 801
BstYI RGATCY 1 cut(s) 204
BstZ17I GTATAC 1 cut(s) 494
BsuI GTATCC 1 cut(s) 817
BsuRI GGCC 1 cut(s) 715
BtgI CCRYGG 1 cut(s) 246
BtsCI GGATG 2 cut(s) 658, 666
BtsIMutI CAGTG 1 cut(s) 709
Cac8I GCNNGC 2 cut(s) 51, 307
CaiI CAGNNNCTG 2 cut(s) 292, 722
Cfr13I GGNCC 2 cut(s) 404, 437
CsiI ACCWGGT 1 cut(s) 285
Csp6I GTAC 2 cut(s) 87, 260
CviAII CATG 6 cut(s) 70, 79, 247, 507, 519, 578
CviQI GTAC 2 cut(s) 87, 260
DdeI CTNAG 1 cut(s) 36
DpnI GATC 1 cut(s) 206
DpnII GATC 1 cut(s) 204
EaeI YGGCCR 1 cut(s) 713
Eco130I CCWWGG 2 cut(s) 246, 571
Eco31I GGTCTC 1 cut(s) 218
Eco47I GGWCC 2 cut(s) 404, 437
Eco57I CTGAAG 1 cut(s) 453
EcoNI CCTNNNNNAGG 1 cut(s) 199
EcoO109I RGGNCCY 1 cut(s) 404
EcoRI GAATTC 1 cut(s) 422
EcoRII CCWGG 1 cut(s) 285
EcoT14I CCWWGG 2 cut(s) 246, 571
EcoT22I ATGCAT 1 cut(s) 666
ErhI CCWWGG 2 cut(s) 246, 571
FaeI CATG 6 cut(s) 73, 82, 250, 510, 522, 581
FalI AAGNNNNNCTT 2 cut(s) 179, 211
FaqI GGGAC 2 cut(s) 390, 775
FatI CATG 6 cut(s) 69, 78, 246, 506, 518, 577
FauI CCCGC 2 cut(s) 246, 650
FauNDI CATATG 1 cut(s) 133
FblI GTMKAC 2 cut(s) 493, 759
Fnu4HI GCNGC 2 cut(s) 19, 639
FokI GGATG 2 cut(s) 665, 673
Fsp4HI GCNGC 2 cut(s) 19, 639
FspBI CTAG 2 cut(s) 62, 470
GluI GCNGC 2 cut(s) 19, 639
HaeIII GGCC 1 cut(s) 715
Hin1II CATG 6 cut(s) 73, 82, 250, 510, 522, 581
HinfI GANTC 2 cut(s) 272, 347
HphI GGTGA 1 cut(s) 216
Hpy166II GTNNAC 2 cut(s) 494, 760
Hpy188I TCNGA 3 cut(s) 28, 277, 550
Hpy188III TCNNGA 7 cut(s) 35, 62, 98, 353, 419, 530, 723
Hpy8I GTNNAC 2 cut(s) 494, 760
HpyAV CCTTC 2 cut(s) 395, 428
HpyCH4III ACNGT 1 cut(s) 302
HpyCH4V TGCA 7 cut(s) 53, 137, 295, 598, 664, 781, 820
HpyF10VI GCNNNNNNNGC 1 cut(s) 46
HpyF3I CTNAG 1 cut(s) 36
Hsp92II CATG 6 cut(s) 73, 82, 250, 510, 522, 581
Kzo9I GATC 1 cut(s) 204
LmnI GCTCC 1 cut(s) 250
Lsp1109I GCAGC 2 cut(s) 30, 625
LweI GCATC 3 cut(s) 651, 673, 790
MabI ACCWGGT 1 cut(s) 285
MaeI CTAG 2 cut(s) 62, 470
MalI GATC 1 cut(s) 206
MbiI CCGCTC 1 cut(s) 253
MboI GATC 1 cut(s) 204
MboII GAAGA 2 cut(s) 553, 563
MflI RGATCY 1 cut(s) 204
MhlI GDGCHC 1 cut(s) 114
MlsI TGGCCA 1 cut(s) 715
MluCI AATT 8 cut(s) 92, 360, 367, 380, 422, 441, 747, 772
MluNI TGGCCA 1 cut(s) 715
MnlI CCTC 6 cut(s) 42, 110, 250, 349, 534, 574
Mox20I TGGCCA 1 cut(s) 715
Mph1103I ATGCAT 1 cut(s) 666
MroXI GAANNNNTTC 1 cut(s) 321
MscI TGGCCA 1 cut(s) 715
MseI TTAA 5 cut(s) 282, 480, 503, 615, 810
MslI CAYNNNNRTG 2 cut(s) 517, 799
Msp20I TGGCCA 1 cut(s) 715
MspR9I CCNGG 1 cut(s) 287
Mva1269I GAATGC 1 cut(s) 598
MvaI CCWGG 1 cut(s) 287
MwoI GCNNNNNNNGC 1 cut(s) 46
NcoI CCATGG 1 cut(s) 246
NdeI CATATG 1 cut(s) 133
NdeII GATC 1 cut(s) 204
NlaIII CATG 6 cut(s) 73, 82, 250, 510, 522, 581
NlaIV GGNNCC 2 cut(s) 193, 406
NsiI ATGCAT 1 cut(s) 666
NspI RCATGY 1 cut(s) 73
PctI GAATGC 1 cut(s) 598
PdmI GAANNNNTTC 1 cut(s) 321
PfeI GAWTC 2 cut(s) 272, 347
PflMI CCANNNNNTGG 1 cut(s) 722
PkrI GCNGC 2 cut(s) 20, 640
PpuMI RGGWCCY 1 cut(s) 404
Psp5II RGGWCCY 1 cut(s) 404
Psp6I CCWGG 1 cut(s) 285
PspGI CCWGG 1 cut(s) 285
PspN4I GGNNCC 2 cut(s) 193, 406
PspPI GGNCC 2 cut(s) 404, 437
PspPPI RGGWCCY 1 cut(s) 404
PstI CTGCAG 2 cut(s) 297, 822
PstNI CAGNNNCTG 2 cut(s) 292, 722
PsuI RGATCY 1 cut(s) 204
RsaI GTAC 2 cut(s) 88, 261
RsaNI GTAC 2 cut(s) 87, 260
RseI CAYNNNNRTG 2 cut(s) 517, 799
SaqAI TTAA 5 cut(s) 282, 480, 503, 615, 810
SatI GCNGC 2 cut(s) 19, 639
Sau3AI GATC 1 cut(s) 204
Sau96I GGNCC 2 cut(s) 404, 437
ScrFI CCNGG 1 cut(s) 287
SduI GDGCHC 1 cut(s) 114
SetI ASST 7 cut(s) 23, 261, 291, 315, 406, 439, 454
SexAI ACCWGGT 1 cut(s) 285
SfaNI GCATC 3 cut(s) 651, 673, 790
SfcI CTRYAG 3 cut(s) 267, 293, 818
SinI GGWCC 2 cut(s) 404, 437
SmiMI CAYNNNNRTG 2 cut(s) 517, 799
SmlI CTYRAG 1 cut(s) 185
SmoI CTYRAG 1 cut(s) 185
Sse9I AATT 8 cut(s) 92, 360, 367, 380, 422, 441, 747, 772
SsiI CCGC 2 cut(s) 253, 657
SspI AATATT 1 cut(s) 557
SspMI CTAG 2 cut(s) 62, 470
StyD4I CCNGG 1 cut(s) 285
StyI CCWWGG 2 cut(s) 246, 571
TaaI ACNGT 1 cut(s) 302
TasI AATT 8 cut(s) 92, 360, 367, 380, 422, 441, 747, 772
TfiI GAWTC 2 cut(s) 272, 347
Tru1I TTAA 5 cut(s) 282, 480, 503, 615, 810
Tru9I TTAA 5 cut(s) 282, 480, 503, 615, 810
TscAI CASTG 1 cut(s) 716
TseI GCWGC 2 cut(s) 18, 638
TspDTI ATGAA 4 cut(s) 67, 117, 255, 693
TspGWI ACGGA 3 cut(s) 104, 129, 711
TspRI CASTG 1 cut(s) 716
Van91I CCANNNNNTGG 1 cut(s) 722
VpaK11BI GGWCC 2 cut(s) 404, 437
XagI CCTNNNNNAGG 1 cut(s) 199
XapI RAATTY 3 cut(s) 92, 422, 772
XbaI TCTAGA 1 cut(s) 61
XceI RCATGY 1 cut(s) 73
XmiI GTMKAC 2 cut(s) 493, 759
XmnI GAANNNNTTC 1 cut(s) 321
XspI CTAG 2 cut(s) 62, 470
Zsp2I ATGCAT 1 cut(s) 666
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.