Rh5AG477000

Belongs to the peptidase M16 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0000891
Physical Location & Seq
Reverse (-)
81898063 .. 81905683
7621 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG477000.1

Sequence Viewer

Length: 1752 bp
ATGTGTGTAGGAATAGGCAGCTTCTCTGACCCTCCTGAGGCACCGGGGCTTGCACACTTTCTAGAACACATGCTCTTTATGGGGAGTACAGAATTTCCAGATGAAAATGAGTATGACAGTTACTTGTCCAAGCATGGAGGGTGTTCAAATGCATACACGGAAGTAGAGCATACTTGCTACCATTTTGAAGTGAAACGAGAGTTTCTCAAGGGTGCCTTGACAAGATTTTCTCAGTTCTTTGTTTCACCCCTTGTAAAAATTGAAGCCATGGAGCGGGAGGTACAGGCTGTAGATTCAGAGTTTAACCAGGTTCTGCAGAACGATGCTTGCCGCCTTGAACAACTTCAATGCCATACAGCCTCACCTGGTCACCCATTTAATAGATTTGCATGGGGGAATAAAAAAAGCTTGAGCAATGCCATGGAAAAAGGGATCAACTTGCGGGAACAAATATTGAAATTGTACAGAGATTATTACCATGGTGGATTGATGAAGCTAGTTGTCATAGGAGGAGGAAATTTACTAATTTATGCAGCAGAACATGTTATTTATGGGTCCTACGCATACAAGATTTGGGATGAGGAATCAATCAAATATGTTCTTGATTTCCTTAGACCAGAAAACATGAGGATTGATGTTGTATCAAAGCCCTCATTTAAGTCAGAAGATTTCCAGTGCGAACCTTGGTTTGGGTCACATTATACTGAGGAAGATATATCTCCATCTTTGATAGATTTGTGGAAGGACCCTCCAGAAATTGATGTTTCATTGCATCTCCCAGAAAAGAATGAGTTCATTCCTACTGATTTTTCCATTCGTTCCGATGGTCTTGATACTACAGATACAGCTTCTCCGAGATGTATACTGGATGAGCCATTGGTGAAGTTCTGGTACAAACTTGATAATACTTTTAAACTACCGCGGGCAAATACTTATTTCCGCATCAATCCCAATGGTGGATATGATAATGTGAAGAGTTGTGTCTTGACTGAACTATATGTATCCCTCCTCAAAGATGAGCTGAATGAGATTGTTTATCAGGCTAGTGTTGCCAAGCTGGAAACTTCGGTATCTGTATCGACTGACAAGCTGGAGCTAAAGGTCTACGGTTTCAATGACAAGCTTCCAGCTCTGTTGTCACAGATTCTGAAAACAACCAAAAGTTTCATGCCAACTTCTGATCGTTTTATGGTTATCAAAGAAGATATGGAGCGAAAGTTAAAGAACACCAATATGAAGCCTCTGAGTCACTCTACATACTTGAGAGTGCAAGTTTTGTTCCAGAGATTCTATGATGTTGATGAGAAGTTGCATTCAGCTGAGTACAACCCAATCTACTTGCAAGGGAGAGTTGAGAACTTTATAAATAGTCTGGAGGAGTTGTTGCAAGGACTGGACGATGACTCTTTTGAGGATTACAGAGCTGGGCTAATTGCAAAGCTGTTGGAGAAAGATCCATCCCTCCATTATGAAACCAATCGATATTGGTTACAGATTACCGATAAAATGTATATGTTTGACTATACAAAACAGGAGGCAGAACGACTCAGGAGCATTCAGAAGGAGGATGTCATCAACTGGTACAAAACATATTTGCAACAATCATCTCCCAAGTGTCGGAGACTTGCAATTCGTGTTTGGGGATGCAACACAGACTTGAAAGAGGCTGAAGCACGGCCGGAGTCTGTACAAGTCATTGAAGACCTTGCAGCCTTTAAGATGTCATCTGAGTTCTATCCAAGCATTTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0003674 GO:0003824 GO:0004175 GO:0004222 GO:0005102 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005777 GO:0005782 GO:0005829 GO:0006508 GO:0006518 GO:0006605 GO:0006625 GO:0006807 GO:0006810 GO:0006886 GO:0006996 GO:0007031 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007169 GO:0007275 GO:0007568 GO:0008104 GO:0008144 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0008270 GO:0008286 GO:0008340 GO:0009056 GO:0009057 GO:0009719 GO:0009725 GO:0009893 GO:0009894 GO:0009896 GO:0009986 GO:0009987 GO:0010033 GO:0010243 GO:0010259 GO:0010604 GO:0010815 GO:0010992 GO:0015031 GO:0015833 GO:0016043 GO:0016787 GO:0017046 GO:0017076 GO:0017144 GO:0019222 GO:0019538 GO:0019725 GO:0022607 GO:0023052 GO:0030163 GO:0030554 GO:0031334 GO:0031907 GO:0031974 GO:0032459 GO:0032461 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0032868 GO:0032869 GO:0032870 GO:0033036 GO:0033218 GO:0033365 GO:0034613 GO:0034641 GO:0035639 GO:0036094 GO:0042176 GO:0042221 GO:0042277 GO:0042562 GO:0042579 GO:0042592 GO:0042737 GO:0042802 GO:0042803 GO:0042886 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043171 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043254 GO:0043434 GO:0043559 GO:0043574 GO:0043603 GO:0043933 GO:0044085 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044421 GO:0044422 GO:0044424 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0045732 GO:0046872 GO:0046907 GO:0046914 GO:0046983 GO:0048518 GO:0048522 GO:0048856 GO:0050435 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051259 GO:0051260 GO:0051603 GO:0051641 GO:0051649 GO:0051716 GO:0060255 GO:0065003 GO:0065007 GO:0065008 GO:0070011 GO:0070013 GO:0070727 GO:0070887 GO:0071310 GO:0071375 GO:0071417 GO:0071495 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072662 GO:0072663 GO:0080090 GO:0097159 GO:0097242 GO:0097367 GO:0140030 GO:0140035 GO:0140036 GO:0140096 GO:1901142 GO:1901143 GO:1901265 GO:1901363 GO:1901564 GO:1901565 GO:1901575 GO:1901652 GO:1901653 GO:1901698 GO:1901699 GO:1901700 GO:1901701
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_M16 Peptidase_M16_C Peptidase_M16_M

Protein Analysis

583

Amino Acids

67.65

Weight (kDa)

5.17

Isoelectric Point (pI)

41.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M16 PF00675 1 - 122 3.4e-30 Insulinase (Peptidase family M16)
Peptidase_M16_M PF16187 173 - 438 3.4e-84 Middle or third domain of peptidase_M16
PqqF-like_C_4 PF22456 439 - 496 2.7e-08 PQQ synthase PqqF-like, C-terminal lobe domain 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1364
AccB1I GGYRCC 2 cut(s) 40, 212
AccBSI CCGCTC 1 cut(s) 274
AccI GTMKAC 2 cut(s) 862, 1104
AccII CGCG 1 cut(s) 922
AciI CCGC 6 cut(s) 274, 331, 442, 920, 922, 940
AclWI GGATC 2 cut(s) 440, 1448
AcoI YGGCCR 1 cut(s) 1676
AcsI RAATTY 2 cut(s) 92, 517
AcuI CTGAAG 1 cut(s) 1689
AfaI GTAC 7 cut(s) 88, 282, 464, 893, 1325, 1583, 1689
AfiI CCNNNNNNNGG 5 cut(s) 37, 273, 689, 956, 1617
AflIII ACRYGT 1 cut(s) 541
AgsI TTSAA 9 cut(s) 147, 188, 263, 338, 347, 457, 1114, 1660, 1700
AjnI CCWGG 2 cut(s) 306, 364
AjuI GAANNNNNNNTTGG 2 cut(s) 672, 704
Alw26I GTCTC 1 cut(s) 1615
AlwI GGATC 2 cut(s) 440, 1448
AlwNI CAGNNNCTG 2 cut(s) 313, 1147
AoxI GGCC 1 cut(s) 1676
ApeKI GCWGC 3 cut(s) 18, 533, 1709
ApoI RAATTY 2 cut(s) 92, 517
ArsI GACNNNNNNTTYG 2 cut(s) 1614, 1646
Asp700I GAANNNNTTC 2 cut(s) 342, 791
AspS9I GGNCC 2 cut(s) 555, 745
AsuC2I CCSGG 1 cut(s) 45
AsuHPI GGTGA 4 cut(s) 237, 354, 362, 892
AvaII GGWCC 2 cut(s) 555, 745
AxyI CCTNAGG 1 cut(s) 36
BaeI ACNNNNGTAYC 2 cut(s) 1053, 1086
BanI GGYRCC 2 cut(s) 40, 212
BarI GAAGNNNNNNTAC 2 cut(s) 875, 907
BbsI GAAGAC 1 cut(s) 1707
BbvI GCAGC 3 cut(s) 30, 545, 1721
BccI CCATC 3 cut(s) 730, 818, 1465
BceAI ACGGC 1 cut(s) 1691
BciT130I CCWGG 2 cut(s) 308, 366
BciVI GTATCC 1 cut(s) 1012
BcnI CCSGG 1 cut(s) 45
BcoDI GTCTC 1 cut(s) 1615
BfaI CTAG 3 cut(s) 62, 497, 1044
BfmI CTRYAG 3 cut(s) 288, 314, 837
BfuI GTATCC 1 cut(s) 1012
BisI GCNGC 4 cut(s) 19, 331, 534, 1710
BlsI GCNGC 4 cut(s) 20, 332, 535, 1711
Bme1390I CCNGG 3 cut(s) 45, 308, 366
Bme18I GGWCC 2 cut(s) 555, 745
BmgT120I GGNCC 2 cut(s) 555, 745
BmiI GGNNCC 4 cut(s) 42, 214, 556, 747
BmrFI CCNGG 3 cut(s) 45, 308, 366
BmsI GCATC 4 cut(s) 313, 781, 951, 1634
BpiI GAAGAC 1 cut(s) 1707
BplI GAGNNNNNCTC 2 cut(s) 189, 221
BpmI CTGGAG 3 cut(s) 735, 1112, 1394
BpuEI CTTGAG 3 cut(s) 191, 430, 1282
BpuMI CCSGG 1 cut(s) 45
Bsa29I ATCGAT 1 cut(s) 1480
BsaJI CCNNGG 6 cut(s) 44, 267, 420, 478, 683, 920
BsaXI ACNNNNNCTCC 6 cut(s) 835, 865, 1202, 1232, 1367, 1397
Bsc4I CCNNNNNNNGG 5 cut(s) 37, 273, 689, 956, 1617
Bse1I ACTGG 4 cut(s) 673, 870, 1398, 1583
Bse21I CCTNAGG 1 cut(s) 36
Bse3DI GCAATG 2 cut(s) 421, 767
BseBI CCWGG 2 cut(s) 308, 366
BseCI ATCGAT 1 cut(s) 1480
BseDI CCNNGG 6 cut(s) 44, 267, 420, 478, 683, 920
BseGI GGATG 5 cut(s) 583, 874, 1457, 1573, 1649
BseLI CCNNNNNNNGG 5 cut(s) 37, 273, 689, 956, 1617
BseMI GCAATG 2 cut(s) 421, 767
BseMII CTCAG 7 cut(s) 27, 245, 696, 1235, 1311, 1561, 1719
BseNI ACTGG 4 cut(s) 673, 870, 1398, 1583
BseRI GAGGAG 3 cut(s) 525, 998, 1391
BseX3I CGGCCG 1 cut(s) 1676
BseXI GCAGC 3 cut(s) 30, 545, 1721
BseYI CCCAGC 1 cut(s) 1424
Bsh1236I CGCG 1 cut(s) 922
Bsh1285I CGRYCG 1 cut(s) 1679
BshFI GGCC 1 cut(s) 1678
BshNI GGYRCC 2 cut(s) 40, 212
BshVI ATCGAT 1 cut(s) 1480
BsiEI CGRYCG 1 cut(s) 1679
BsiSI CCGG 2 cut(s) 44, 1679
BslI CCNNNNNNNGG 5 cut(s) 37, 273, 689, 956, 1617
BsmAI GTCTC 1 cut(s) 1615
BsmI GAATGC 2 cut(s) 1312, 1554
BsnI GGCC 1 cut(s) 1678
Bsp1407I TGTACA 2 cut(s) 462, 1687
Bsp143I GATC 3 cut(s) 432, 1180, 1453
Bsp19I CCATGG 3 cut(s) 267, 420, 478
BspACI CCGC 6 cut(s) 274, 331, 442, 920, 922, 940
BspANI GGCC 1 cut(s) 1678
BspCNI CTCAG 7 cut(s) 28, 244, 697, 1236, 1312, 1560, 1720
BspDI ATCGAT 1 cut(s) 1480
BspFNI CGCG 1 cut(s) 922
BspLI GGNNCC 4 cut(s) 42, 214, 556, 747
BspMAI CTGCAG 1 cut(s) 318
BspPI GGATC 2 cut(s) 440, 1448
BspT107I GGYRCC 2 cut(s) 40, 212
BsrBI CCGCTC 1 cut(s) 274
BsrDI GCAATG 2 cut(s) 421, 767
BsrGI TGTACA 2 cut(s) 462, 1687
BsrI ACTGG 4 cut(s) 673, 870, 1398, 1583
BssECI CCNNGG 6 cut(s) 44, 267, 420, 478, 683, 920
BssMI GATC 3 cut(s) 432, 1180, 1453
BssNAI GTATAC 1 cut(s) 863
BssT1I CCWWGG 4 cut(s) 267, 420, 478, 683
Bst1107I GTATAC 1 cut(s) 863
Bst2UI CCWGG 2 cut(s) 308, 366
Bst4CI ACNGT 2 cut(s) 119, 1109
Bst6I CTCTTC 1 cut(s) 968
BstAUI TGTACA 2 cut(s) 462, 1687
BstC8I GCNNGC 3 cut(s) 51, 328, 924
BstDEI CTNAG 8 cut(s) 36, 231, 611, 705, 1244, 1320, 1547, 1728
BstDSI CCRYGG 4 cut(s) 267, 420, 478, 920
BstEII GGTNACC 1 cut(s) 368
BstF5I GGATG 5 cut(s) 583, 874, 1457, 1573, 1649
BstFNI CGCG 1 cut(s) 922
BstKTI GATC 3 cut(s) 435, 1183, 1456
BstMAI GTCTC 1 cut(s) 1615
BstMBI GATC 3 cut(s) 432, 1180, 1453
BstMCI CGRYCG 1 cut(s) 1679
BstMWI GCNNNNNNNGC 1 cut(s) 1049
BstNI CCWGG 2 cut(s) 308, 366
BstNSI RCATGY 2 cut(s) 73, 545
BstPI GGTNACC 1 cut(s) 368
BstSCI CCNGG 3 cut(s) 43, 306, 364
BstSFI CTRYAG 3 cut(s) 288, 314, 837
BstUI CGCG 1 cut(s) 922
BstV1I GCAGC 3 cut(s) 30, 545, 1721
BstV2I GAAGAC 1 cut(s) 1707
BstX2I RGATCY 1 cut(s) 1453
BstYI RGATCY 1 cut(s) 1453
BstZ17I GTATAC 1 cut(s) 863
BstZI CGGCCG 1 cut(s) 1676
Bsu15I ATCGAT 1 cut(s) 1480
Bsu36I CCTNAGG 1 cut(s) 36
BsuI GTATCC 1 cut(s) 1012
BsuRI GGCC 1 cut(s) 1678
BsuTUI ATCGAT 1 cut(s) 1480
BtgI CCRYGG 4 cut(s) 267, 420, 478, 920
BtsCI GGATG 5 cut(s) 583, 874, 1457, 1573, 1649
BtsIMutI CAGTG 1 cut(s) 680
Cac8I GCNNGC 3 cut(s) 51, 328, 924
CaiI CAGNNNCTG 2 cut(s) 313, 1147
Cfr13I GGNCC 2 cut(s) 555, 745
Cfr42I CCGCGG 1 cut(s) 923
ClaI ATCGAT 1 cut(s) 1480
CsiI ACCWGGT 2 cut(s) 306, 364
Csp6I GTAC 7 cut(s) 87, 281, 463, 892, 1324, 1582, 1688
CviAII CATG 9 cut(s) 70, 134, 268, 390, 421, 479, 542, 625, 1168
CviQI GTAC 7 cut(s) 87, 281, 463, 892, 1324, 1582, 1688
DdeI CTNAG 8 cut(s) 36, 231, 611, 705, 1244, 1320, 1547, 1728
DpnI GATC 3 cut(s) 434, 1182, 1455
DpnII GATC 3 cut(s) 432, 1180, 1453
DraI TTTAAA 1 cut(s) 913
EaeI YGGCCR 1 cut(s) 1676
EagI CGGCCG 1 cut(s) 1676
Eam1104I CTCTTC 1 cut(s) 968
EarI CTCTTC 1 cut(s) 968
EclXI CGGCCG 1 cut(s) 1676
Eco130I CCWWGG 4 cut(s) 267, 420, 478, 683
Eco47I GGWCC 2 cut(s) 555, 745
Eco52I CGGCCG 1 cut(s) 1676
Eco57I CTGAAG 1 cut(s) 1689
Eco81I CCTNAGG 1 cut(s) 36
Eco91I GGTNACC 1 cut(s) 368
EcoO109I RGGNCCY 2 cut(s) 555, 745
EcoO65I GGTNACC 1 cut(s) 368
EcoRII CCWGG 2 cut(s) 306, 364
EcoT14I CCWWGG 4 cut(s) 267, 420, 478, 683
EcoT22I ATGCAT 1 cut(s) 154
ErhI CCWWGG 4 cut(s) 267, 420, 478, 683
FaeI CATG 9 cut(s) 73, 137, 271, 393, 424, 482, 545, 628, 1171
FalI AAGNNNNNCTT 2 cut(s) 200, 232
FatI CATG 9 cut(s) 69, 133, 267, 389, 420, 478, 541, 624, 1167
FauI CCCGC 3 cut(s) 267, 435, 915
FblI GTMKAC 2 cut(s) 862, 1104
Fnu4HI GCNGC 4 cut(s) 19, 331, 534, 1710
FokI GGATG 5 cut(s) 590, 881, 1444, 1580, 1656
Fsp4HI GCNGC 4 cut(s) 19, 331, 534, 1710
FspBI CTAG 3 cut(s) 62, 497, 1044
GluI GCNGC 4 cut(s) 19, 331, 534, 1710
GsaI CCCAGC 1 cut(s) 1428
GsuI CTGGAG 3 cut(s) 735, 1112, 1394
HaeIII GGCC 1 cut(s) 1678
HapII CCGG 2 cut(s) 44, 1679
Hin1II CATG 9 cut(s) 73, 137, 271, 393, 424, 482, 545, 628, 1171
HindIII AAGCTT 2 cut(s) 406, 1121
HinfI GANTC 8 cut(s) 293, 584, 1144, 1246, 1287, 1403, 1545, 1682
HpaII CCGG 2 cut(s) 44, 1679
HphI GGTGA 4 cut(s) 237, 354, 362, 892
Hpy166II GTNNAC 2 cut(s) 863, 1105
Hpy8I GTNNAC 2 cut(s) 863, 1105
HpyAV CCTTC 2 cut(s) 736, 1555
HpyCH4III ACNGT 2 cut(s) 119, 1109
HpyF10VI GCNNNNNNNGC 1 cut(s) 1049
HpyF3I CTNAG 8 cut(s) 36, 231, 611, 705, 1244, 1320, 1547, 1728
Hsp92II CATG 9 cut(s) 73, 137, 271, 393, 424, 482, 545, 628, 1171
KspI CCGCGG 1 cut(s) 923
Kzo9I GATC 3 cut(s) 432, 1180, 1453
LmnI GCTCC 4 cut(s) 271, 1093, 1210, 1551
Lsp1109I GCAGC 3 cut(s) 30, 545, 1721
LweI GCATC 4 cut(s) 313, 781, 951, 1634
MabI ACCWGGT 2 cut(s) 306, 364
MaeI CTAG 3 cut(s) 62, 497, 1044
MaeIII GTNAC 6 cut(s) 119, 368, 693, 1137, 1247, 1488
MalI GATC 3 cut(s) 434, 1182, 1455
MbiI CCGCTC 1 cut(s) 274
MboI GATC 3 cut(s) 432, 1180, 1453
MboII GAAGA 5 cut(s) 677, 722, 985, 1214, 1712
MflI RGATCY 1 cut(s) 1453
MluCI AATT 8 cut(s) 92, 258, 458, 517, 525, 756, 1431, 1629
MlyI GAGTC 4 cut(s) 1255, 1397, 1539, 1691
MmeI TCCRAC 2 cut(s) 1425, 1598
Mph1103I ATGCAT 1 cut(s) 154
MroXI GAANNNNTTC 2 cut(s) 342, 791
MseI TTAA 6 cut(s) 303, 378, 657, 912, 1220, 1716
MslI CAYNNNNRTG 1 cut(s) 1232
MspA1I CMGCKG 2 cut(s) 922, 1319
MspI CCGG 2 cut(s) 44, 1679
MspR9I CCNGG 3 cut(s) 45, 308, 366
Mva1269I GAATGC 2 cut(s) 1312, 1554
MvaI CCWGG 2 cut(s) 308, 366
MvnI CGCG 1 cut(s) 922
MwoI GCNNNNNNNGC 1 cut(s) 1049
NciI CCSGG 1 cut(s) 45
NcoI CCATGG 3 cut(s) 267, 420, 478
NdeII GATC 3 cut(s) 432, 1180, 1453
NlaIII CATG 9 cut(s) 73, 137, 271, 393, 424, 482, 545, 628, 1171
NlaIV GGNNCC 4 cut(s) 42, 214, 556, 747
NmuCI GTSAC 4 cut(s) 368, 693, 1137, 1247
NsiI ATGCAT 1 cut(s) 154
NspI RCATGY 2 cut(s) 73, 545
PciI ACATGT 1 cut(s) 541
PctI GAATGC 2 cut(s) 1312, 1554
PdmI GAANNNNTTC 2 cut(s) 342, 791
PfeI GAWTC 4 cut(s) 293, 584, 1144, 1287
PkrI GCNGC 4 cut(s) 20, 332, 535, 1711
PleI GAGTC 4 cut(s) 1254, 1397, 1539, 1690
PpsI GAGTC 4 cut(s) 1254, 1397, 1539, 1690
PpuMI RGGWCCY 2 cut(s) 555, 745
PscI ACATGT 1 cut(s) 541
PsiI TTATAA 1 cut(s) 1364
Psp5II RGGWCCY 2 cut(s) 555, 745
Psp6I CCWGG 2 cut(s) 306, 364
PspEI GGTNACC 1 cut(s) 368
PspFI CCCAGC 1 cut(s) 1424
PspGI CCWGG 2 cut(s) 306, 364
PspN4I GGNNCC 4 cut(s) 42, 214, 556, 747
PspPI GGNCC 2 cut(s) 555, 745
PspPPI RGGWCCY 2 cut(s) 555, 745
PstI CTGCAG 1 cut(s) 318
PstNI CAGNNNCTG 2 cut(s) 313, 1147
PsuI RGATCY 1 cut(s) 1453
PvuII CAGCTG 1 cut(s) 1319
RsaI GTAC 7 cut(s) 88, 282, 464, 893, 1325, 1583, 1689
RsaNI GTAC 7 cut(s) 87, 281, 463, 892, 1324, 1582, 1688
RseI CAYNNNNRTG 1 cut(s) 1232
SacII CCGCGG 1 cut(s) 923
SaqAI TTAA 6 cut(s) 303, 378, 657, 912, 1220, 1716
SatI GCNGC 4 cut(s) 19, 331, 534, 1710
Sau3AI GATC 3 cut(s) 432, 1180, 1453
Sau96I GGNCC 2 cut(s) 555, 745
SchI GAGTC 4 cut(s) 1255, 1397, 1539, 1691
ScrFI CCNGG 3 cut(s) 45, 308, 366
SexAI ACCWGGT 2 cut(s) 306, 364
SfaNI GCATC 4 cut(s) 313, 781, 951, 1634
SfcI CTRYAG 3 cut(s) 288, 314, 837
Sfr303I CCGCGG 1 cut(s) 923
SgrBI CCGCGG 1 cut(s) 923
SinI GGWCC 2 cut(s) 555, 745
SmiMI CAYNNNNRTG 1 cut(s) 1232
SmlI CTYRAG 3 cut(s) 206, 409, 1261
SmoI CTYRAG 3 cut(s) 206, 409, 1261
Sse9I AATT 8 cut(s) 92, 258, 458, 517, 525, 756, 1431, 1629
SsiI CCGC 6 cut(s) 274, 331, 442, 920, 922, 940
SspI AATATT 1 cut(s) 453
SspMI CTAG 3 cut(s) 62, 497, 1044
StyD4I CCNGG 3 cut(s) 43, 306, 364
StyI CCWWGG 4 cut(s) 267, 420, 478, 683
TaaI ACNGT 2 cut(s) 119, 1109
TaqI TCGA 2 cut(s) 1079, 1480
TasI AATT 8 cut(s) 92, 258, 458, 517, 525, 756, 1431, 1629
TatI WGTACW 4 cut(s) 86, 462, 1323, 1687
TauI GCSGC 1 cut(s) 333
TfiI GAWTC 4 cut(s) 293, 584, 1144, 1287
Tru1I TTAA 6 cut(s) 303, 378, 657, 912, 1220, 1716
Tru9I TTAA 6 cut(s) 303, 378, 657, 912, 1220, 1716
TscAI CASTG 1 cut(s) 680
TseFI GTSAC 4 cut(s) 368, 693, 1137, 1247
TseI GCWGC 3 cut(s) 18, 533, 1709
Tsp45I GTSAC 4 cut(s) 368, 693, 1137, 1247
TspDTI ATGAA 7 cut(s) 117, 506, 756, 784, 1156, 1250, 1485
TspGWI ACGGA 1 cut(s) 173
TspRI CASTG 1 cut(s) 680
VpaK11BI GGWCC 2 cut(s) 555, 745
XapI RAATTY 2 cut(s) 92, 517
XbaI TCTAGA 1 cut(s) 61
XceI RCATGY 2 cut(s) 73, 545
XmiI GTMKAC 2 cut(s) 862, 1104
XmnI GAANNNNTTC 2 cut(s) 342, 791
XspI CTAG 3 cut(s) 62, 497, 1044
Zsp2I ATGCAT 1 cut(s) 154
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.