Belongs to the peptidase M16 family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 1 cut(s) | 319 |
| AcsI | RAATTY | 1 cut(s) | 117 |
| AfaI | GTAC | 1 cut(s) | 210 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 291 |
| AflII | CTTAAG | 1 cut(s) | 94 |
| AflIII | ACRYGT | 1 cut(s) | 357 |
| AgsI | TTSAA | 1 cut(s) | 178 |
| AjnI | CCWGG | 1 cut(s) | 204 |
| AleI | CACNNNNGTG | 1 cut(s) | 221 |
| AluBI | AGCT | 1 cut(s) | 196 |
| AluI | AGCT | 1 cut(s) | 196 |
| Ama87I | CYCGRG | 1 cut(s) | 191 |
| ApoI | RAATTY | 1 cut(s) | 117 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 182 |
| AsuC2I | CCSGG | 1 cut(s) | 293 |
| AvaI | CYCGRG | 1 cut(s) | 191 |
| BciT130I | CCWGG | 1 cut(s) | 206 |
| BcnI | CCSGG | 1 cut(s) | 293 |
| BfrI | CTTAAG | 1 cut(s) | 94 |
| Bme1390I | CCNGG | 2 cut(s) | 206, 293 |
| BmeT110I | CYCGRG | 1 cut(s) | 191 |
| BmrFI | CCNGG | 2 cut(s) | 206, 293 |
| BmsI | GCATC | 1 cut(s) | 118 |
| BpmI | CTGGAG | 1 cut(s) | 271 |
| BpuEI | CTTGAG | 1 cut(s) | 251 |
| BpuMI | CCSGG | 1 cut(s) | 293 |
| BsaJI | CCNNGG | 1 cut(s) | 204 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 291 |
| BseBI | CCWGG | 1 cut(s) | 206 |
| BseDI | CCNNGG | 1 cut(s) | 204 |
| BseGI | GGATG | 2 cut(s) | 160, 281 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 291 |
| BsiHKCI | CYCGRG | 1 cut(s) | 191 |
| BsiSI | CCGG | 1 cut(s) | 292 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 291 |
| BsoBI | CYCGRG | 1 cut(s) | 191 |
| Bsp143I | GATC | 1 cut(s) | 13 |
| BspACI | CCGC | 1 cut(s) | 319 |
| BspHI | TCATGA | 1 cut(s) | 304 |
| BspTI | CTTAAG | 1 cut(s) | 94 |
| BssECI | CCNNGG | 1 cut(s) | 204 |
| BssMI | GATC | 1 cut(s) | 13 |
| Bst2UI | CCWGG | 1 cut(s) | 206 |
| Bst4CI | ACNGT | 1 cut(s) | 248 |
| BstAFI | CTTAAG | 1 cut(s) | 94 |
| BstDEI | CTNAG | 1 cut(s) | 77 |
| BstF5I | GGATG | 2 cut(s) | 160, 281 |
| BstKTI | GATC | 1 cut(s) | 16 |
| BstMBI | GATC | 1 cut(s) | 13 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 325 |
| BstNI | CCWGG | 1 cut(s) | 206 |
| BstNSI | RCATGY | 2 cut(s) | 149, 361 |
| BstSCI | CCNGG | 2 cut(s) | 204, 291 |
| BtsCI | GGATG | 2 cut(s) | 160, 281 |
| CciI | TCATGA | 1 cut(s) | 304 |
| Csp6I | GTAC | 1 cut(s) | 209 |
| CviAII | CATG | 5 cut(s) | 146, 305, 316, 340, 358 |
| CviJI | RGCY | 4 cut(s) | 73, 196, 291, 328 |
| CviKI_1 | RGCY | 4 cut(s) | 73, 196, 291, 328 |
| CviQI | GTAC | 1 cut(s) | 209 |
| DdeI | CTNAG | 1 cut(s) | 77 |
| DpnI | GATC | 1 cut(s) | 15 |
| DpnII | GATC | 1 cut(s) | 13 |
| Eco88I | CYCGRG | 1 cut(s) | 191 |
| EcoRII | CCWGG | 1 cut(s) | 204 |
| FaeI | CATG | 5 cut(s) | 149, 308, 319, 343, 361 |
| FatI | CATG | 5 cut(s) | 145, 304, 315, 339, 357 |
| FokI | GGATG | 2 cut(s) | 167, 268 |
| GsuI | CTGGAG | 1 cut(s) | 271 |
| HapII | CCGG | 1 cut(s) | 292 |
| Hin1II | CATG | 5 cut(s) | 149, 308, 319, 343, 361 |
| HpaII | CCGG | 1 cut(s) | 292 |
| Hpy166II | GTNNAC | 1 cut(s) | 211 |
| Hpy188I | TCNGA | 1 cut(s) | 172 |
| Hpy188III | TCNNGA | 3 cut(s) | 191, 305, 332 |
| Hpy8I | GTNNAC | 1 cut(s) | 211 |
| HpyAV | CCTTC | 1 cut(s) | 42 |
| HpyCH4III | ACNGT | 1 cut(s) | 248 |
| HpyCH4V | TGCA | 3 cut(s) | 103, 131, 145 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 325 |
| HpyF3I | CTNAG | 1 cut(s) | 77 |
| Hsp92II | CATG | 5 cut(s) | 149, 308, 319, 343, 361 |
| Kzo9I | GATC | 1 cut(s) | 13 |
| LpnPI | CCDG | 6 cut(s) | 191, 218, 225, 301, 305, 310 |
| LweI | GCATC | 1 cut(s) | 118 |
| MaeIII | GTNAC | 3 cut(s) | 80, 235, 242 |
| MalI | GATC | 1 cut(s) | 15 |
| MboI | GATC | 1 cut(s) | 13 |
| MboII | GAAGA | 1 cut(s) | 47 |
| MluCI | AATT | 3 cut(s) | 117, 158, 254 |
| MnlI | CCTC | 3 cut(s) | 84, 295, 347 |
| MroXI | GAANNNNTTC | 1 cut(s) | 182 |
| MseI | TTAA | 5 cut(s) | 21, 30, 53, 95, 275 |
| MslI | CAYNNNNRTG | 2 cut(s) | 65, 221 |
| MspCI | CTTAAG | 1 cut(s) | 94 |
| MspI | CCGG | 1 cut(s) | 292 |
| MspR9I | CCNGG | 2 cut(s) | 206, 293 |
| MvaI | CCWGG | 1 cut(s) | 206 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 325 |
| NciI | CCSGG | 1 cut(s) | 293 |
| NdeII | GATC | 1 cut(s) | 13 |
| NlaIII | CATG | 5 cut(s) | 149, 308, 319, 343, 361 |
| NmuCI | GTSAC | 1 cut(s) | 80 |
| NspI | RCATGY | 2 cut(s) | 149, 361 |
| OliI | CACNNNNGTG | 1 cut(s) | 221 |
| PaeR7I | CTCGAG | 1 cut(s) | 191 |
| PagI | TCATGA | 1 cut(s) | 304 |
| PciI | ACATGT | 1 cut(s) | 357 |
| PdmI | GAANNNNTTC | 1 cut(s) | 182 |
| PscI | ACATGT | 1 cut(s) | 357 |
| Psp6I | CCWGG | 1 cut(s) | 204 |
| PspGI | CCWGG | 1 cut(s) | 204 |
| RsaI | GTAC | 1 cut(s) | 210 |
| RsaNI | GTAC | 1 cut(s) | 209 |
| RseI | CAYNNNNRTG | 2 cut(s) | 65, 221 |
| SaqAI | TTAA | 5 cut(s) | 21, 30, 53, 95, 275 |
| Sau3AI | GATC | 1 cut(s) | 13 |
| ScrFI | CCNGG | 2 cut(s) | 206, 293 |
| SetI | ASST | 7 cut(s) | 27, 53, 109, 198, 210, 244, 339 |
| SfaNI | GCATC | 1 cut(s) | 118 |
| Sfr274I | CTCGAG | 1 cut(s) | 191 |
| SlaI | CTCGAG | 1 cut(s) | 191 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 65, 221 |
| SmlI | CTYRAG | 3 cut(s) | 94, 191, 266 |
| SmoI | CTYRAG | 3 cut(s) | 94, 191, 266 |
| Sse9I | AATT | 3 cut(s) | 117, 158, 254 |
| SsiI | CCGC | 1 cut(s) | 319 |
| StyD4I | CCNGG | 2 cut(s) | 204, 291 |
| TaaI | ACNGT | 1 cut(s) | 248 |
| TaqI | TCGA | 1 cut(s) | 192 |
| TasI | AATT | 3 cut(s) | 117, 158, 254 |
| Tru1I | TTAA | 5 cut(s) | 21, 30, 53, 95, 275 |
| Tru9I | TTAA | 5 cut(s) | 21, 30, 53, 95, 275 |
| TseFI | GTSAC | 1 cut(s) | 80 |
| Tsp45I | GTSAC | 1 cut(s) | 80 |
| TspDTI | ATGAA | 5 cut(s) | 56, 83, 171, 175, 293 |
| Vha464I | CTTAAG | 1 cut(s) | 94 |
| XapI | RAATTY | 1 cut(s) | 117 |
| XceI | RCATGY | 2 cut(s) | 149, 361 |
| XhoI | CTCGAG | 1 cut(s) | 191 |
| XmnI | GAANNNNTTC | 1 cut(s) | 182 |