RchiOBHm_Chr1g0333871

Belongs to the peptidase M16 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000891
Physical Location & Seq
Reverse (-)
25999530 .. 26005491
5962 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ56265

Sequence Viewer

Length: 1770 bp
ATGGGGAGTATAGAATTTCCAAATGAAAATGAGTTCTTTGTTTCACCCCTTGTAAAAAATGAAGCCATGGAGCAGGAGGTTCAGGCTGCAGATTCAGGGAATAAAAAGAGCTTGAGTGTTGCCATGGAAAAAGGGATCAACTTGCGGGAACAAATATTGAAATTGTACACAGATTATTACCATGGTGAATTGATGAAGCTAGTTGTAATAGGTGGAGAGGACCAGAGGTTGATGTTGGAGAACACAATTAAGCATTGTGGGTTTGTCAGTAATGGTGTTCATAAATCTCTTGATGTACTCGAGAATTGGGTTTTGGAATTGTTTGGTAACGTCAAAAAAGGTCCCCCAGTAAATCTGGAATTCAAGGCTGAAGGTCCAATTTGGAAAGCTGGAAAACTTTACAGGCTAGAGGCTATTAAAGATGTTCATATACTCCACTTAACATGGACATTGCCATGCCTTCGTCAAGACTATTTGAAGAAATCAGAAGATTATTTATCTCATCTCCTTGGGCATGCAACATCTCTGGCTGCTGGTGTTGGGGATGACGGGATGCATCATTCTTTTTTGGCGTATGTCTTTCGCATGGACATTCATCTCACTGACTCTGGATTGGATAAGATTTTTGATATAATTGGCTTAGTCTACCGATACATAAAGTTATTGCATCAAGTGTCCCCACAACAATGGATATTTAAGGAACTGCAGGATACTGGGAACATGGATTTTAGATTTACAGAGGAGCAGCCTCAGATGATTATGCCTCAGAACTTGCAGGAAGTGTCTCATAGATGGTTTTTTCGTGTTATCCCTTGTAATTTTATTATTTGTGACTCAGAGTGGACCATGATTTCTCTGTCTATGTATATGTTAACTCATGCCATAAGCATTTGTTTTCTGGATTTCCGATGCGAACCTTGGTTTGGGTCACATTATATTGAGGAAGATATATCTCCATCTTTGATAGATTTGTGGAAGGACCCTCCAGAAATTGATGTTTCATTGCATCTCCCAGAAAAGAATGAGTTCATTCCTACTGATTTTTCCATCTGTTCTGATGGTCTTGATACTACAAATACAGCTTCTCCGAGATGTTTACTGGATGAGCCATCGGCTAGTGTTGCCAAGCTGGAAACTTTGGTATCTGTATCGACTGACAGGCTGGAGCTAAGGGTCTATGGTTTCAATGACAAGCTTCCAGCTCTGTTGTCAAAGATTCTGAAAACAACCAAAAGTTTCATGCCAATTTCTGATCGTTTTATGCCTCTGAGTCACTCTACATACTTAAGAGTGCAAGTTTTGTTCCAGAGATTGTATGATGTTGATGAGAAGTTGCACATTTTAAGTGGACTTTCTATTTCTGATGTGAAGTCATTTATTCCTCAGCTTTGGTCCAAGAATTATTTCAACTTACTGTCTCCTCAATATGCACGGGTGTACATTGAAGGCCTCTGTCATGGAAATTTGTCAGAAAAAGAAGCAATCAGGCTTTCAGATATATTTAAAACAAATTTTGGTGTACAACCACTTCCAATTGAACTGATGCATAGTGAACAGTGTATTTGTCTTCCTCCTGCTAACCTCATTAGAGATGTCAGTGTGAAGAATAAGACGAAGGAGCAGCTAGGTTATACTGTTCATTGTGGCCCGTGGGTTACTTGCAATATATTTGGATTTTATTTTTATGTTCAGTCAGCTGAGTACATCCCAATCTGCTTGCAAGGGACAGTGGAGAACTTTATAAATAGACTGGAGGAGTTGTTGCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0003674 GO:0003824 GO:0004175 GO:0004222 GO:0005102 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005777 GO:0005782 GO:0005829 GO:0006508 GO:0006518 GO:0006605 GO:0006625 GO:0006807 GO:0006810 GO:0006886 GO:0006996 GO:0007031 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007169 GO:0007275 GO:0007568 GO:0008104 GO:0008144 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0008270 GO:0008286 GO:0008340 GO:0009056 GO:0009057 GO:0009719 GO:0009725 GO:0009893 GO:0009894 GO:0009896 GO:0009986 GO:0009987 GO:0010033 GO:0010243 GO:0010259 GO:0010604 GO:0010815 GO:0010992 GO:0015031 GO:0015833 GO:0016043 GO:0016787 GO:0017046 GO:0017076 GO:0017144 GO:0019222 GO:0019538 GO:0019725 GO:0022607 GO:0023052 GO:0030163 GO:0030554 GO:0031334 GO:0031907 GO:0031974 GO:0032459 GO:0032461 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0032868 GO:0032869 GO:0032870 GO:0033036 GO:0033218 GO:0033365 GO:0034613 GO:0034641 GO:0035639 GO:0036094 GO:0042176 GO:0042221 GO:0042277 GO:0042562 GO:0042579 GO:0042592 GO:0042737 GO:0042802 GO:0042803 GO:0042886 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043171 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043254 GO:0043434 GO:0043559 GO:0043574 GO:0043603 GO:0043933 GO:0044085 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044421 GO:0044422 GO:0044424 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0045732 GO:0046872 GO:0046907 GO:0046914 GO:0046983 GO:0048518 GO:0048522 GO:0048856 GO:0050435 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051259 GO:0051260 GO:0051603 GO:0051641 GO:0051649 GO:0051716 GO:0060255 GO:0065003 GO:0065007 GO:0065008 GO:0070011 GO:0070013 GO:0070727 GO:0070887 GO:0071310 GO:0071375 GO:0071417 GO:0071495 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072662 GO:0072663 GO:0080090 GO:0097159 GO:0097242 GO:0097367 GO:0140030 GO:0140035 GO:0140036 GO:0140096 GO:1901142 GO:1901143 GO:1901265 GO:1901363 GO:1901564 GO:1901565 GO:1901575 GO:1901652 GO:1901653 GO:1901698 GO:1901699 GO:1901700 GO:1901701
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_M16 Peptidase_M16_C Peptidase_M16_M

Protein Analysis

589

Amino Acids

67.68

Weight (kDa)

5.42

Isoelectric Point (pI)

42.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M16_M PF16187 304 - 370 1.5e-13 Middle or third domain of peptidase_M16
Peptidase_M16_M PF16187 372 - 424 2.8e-09 Middle or third domain of peptidase_M16
PqqF-like_C_4 PF22456 538 - 588 3.5e-07 PQQ synthase PqqF-like, C-terminal lobe domain 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1742
AccI GTMKAC 1 cut(s) 645
AciI CCGC 1 cut(s) 145
AclWI GGATC 1 cut(s) 143
AcsI RAATTY 4 cut(s) 14, 359, 1462, 1510
AcuI CTGAAG 1 cut(s) 390
AfaI GTAC 5 cut(s) 167, 297, 1439, 1521, 1703
AfiI CCNNNNNNNGG 1 cut(s) 923
AflII CTTAAG 1 cut(s) 1285
AgsI TTSAA 7 cut(s) 160, 364, 478, 1186, 1408, 1445, 1538
AjuI GAANNNNNNNTTGG 6 cut(s) 296, 328, 906, 938, 1388, 1420
Alw26I GTCTC 2 cut(s) 789, 1422
AlwI GGATC 1 cut(s) 143
Ama87I CYCGRG 1 cut(s) 299
AoxI GGCC 2 cut(s) 1447, 1645
ApeKI GCWGC 4 cut(s) 86, 530, 745, 1621
ApoI RAATTY 4 cut(s) 14, 359, 1462, 1510
Asp700I GAANNNNTTC 2 cut(s) 1025, 1403
AspS9I GGNCC 7 cut(s) 220, 341, 374, 843, 979, 1392, 1646
AsuHPI GGTGA 2 cut(s) 36, 197
AvaI CYCGRG 1 cut(s) 299
AvaII GGWCC 6 cut(s) 220, 341, 374, 843, 979, 1392
BaeI ACNNNNGTAYC 2 cut(s) 1125, 1158
BarI GAAGNNNNNNTAC 2 cut(s) 1512, 1544
BbsI GAAGAC 1 cut(s) 1559
BbvCI CCTCAGC 1 cut(s) 1383
BbvI GCAGC 4 cut(s) 73, 517, 757, 1633
BccI CCATC 5 cut(s) 786, 964, 1052, 1055, 1117
BciVI GTATCC 1 cut(s) 703
BcoDI GTCTC 2 cut(s) 789, 1422
BfaI CTAG 4 cut(s) 200, 407, 1116, 1625
BfmI CTRYAG 2 cut(s) 87, 704
BfrI CTTAAG 1 cut(s) 1285
BfuI GTATCC 1 cut(s) 703
BisI GCNGC 4 cut(s) 87, 531, 746, 1622
BlsI GCNGC 4 cut(s) 88, 532, 747, 1623
Bme18I GGWCC 6 cut(s) 220, 341, 374, 843, 979, 1392
BmeT110I CYCGRG 1 cut(s) 299
BmgT120I GGNCC 7 cut(s) 220, 341, 374, 843, 979, 1392, 1646
BmiI GGNNCC 2 cut(s) 343, 981
BmrI ACTGGG 2 cut(s) 341, 723
BmsI GCATC 6 cut(s) 543, 565, 676, 899, 1015, 1533
BmuI ACTGGG 2 cut(s) 341, 723
BpiI GAAGAC 1 cut(s) 1559
BpmI CTGGAG 2 cut(s) 969, 1184
Bpu10I CCTNAGC 2 cut(s) 1169, 1383
BpuEI CTTGAG 1 cut(s) 133
BsaJI CCNNGG 6 cut(s) 66, 123, 181, 508, 917, 1649
BsaXI ACNNNNNCTCC 3 cut(s) 1069, 1099, 1745
Bsc4I CCNNNNNNNGG 1 cut(s) 923
Bse1I ACTGG 4 cut(s) 347, 718, 1104, 1755
Bse3DI GCAATG 2 cut(s) 449, 1001
BseDI CCNNGG 6 cut(s) 66, 123, 181, 508, 917, 1649
BseGI GGATG 4 cut(s) 550, 558, 1108, 1704
BseLI CCNNNNNNNGG 1 cut(s) 923
BseMI GCAATG 2 cut(s) 449, 1001
BseMII CTCAG 6 cut(s) 764, 779, 849, 1259, 1397, 1689
BseNI ACTGG 4 cut(s) 347, 718, 1104, 1755
BseRI GAGGAG 3 cut(s) 755, 1410, 1769
BseXI GCAGC 4 cut(s) 73, 517, 757, 1633
BshFI GGCC 2 cut(s) 1449, 1647
BsiHKCI CYCGRG 1 cut(s) 299
BslFI GGGAC 3 cut(s) 327, 661, 1738
BslI CCNNNNNNNGG 1 cut(s) 923
BsmAI GTCTC 2 cut(s) 789, 1422
BsmFI GGGAC 3 cut(s) 327, 661, 1738
BsnI GGCC 2 cut(s) 1449, 1647
BsoBI CYCGRG 1 cut(s) 299
Bsp1407I TGTACA 3 cut(s) 165, 1437, 1519
Bsp143I GATC 2 cut(s) 135, 1252
Bsp19I CCATGG 3 cut(s) 66, 123, 181
BspACI CCGC 1 cut(s) 145
BspANI GGCC 2 cut(s) 1449, 1647
BspCNI CTCAG 6 cut(s) 763, 778, 848, 1260, 1396, 1690
BspLI GGNNCC 2 cut(s) 343, 981
BspMAI CTGCAG 2 cut(s) 91, 708
BspPI GGATC 1 cut(s) 143
BspTI CTTAAG 1 cut(s) 1285
BsrDI GCAATG 2 cut(s) 449, 1001
BsrGI TGTACA 3 cut(s) 165, 1437, 1519
BsrI ACTGG 4 cut(s) 347, 718, 1104, 1755
BssECI CCNNGG 6 cut(s) 66, 123, 181, 508, 917, 1649
BssMI GATC 2 cut(s) 135, 1252
BssT1I CCWWGG 5 cut(s) 66, 123, 181, 508, 917
Bst4CI ACNGT 4 cut(s) 1416, 1557, 1636, 1729
BstAFI CTTAAG 1 cut(s) 1285
BstAUI TGTACA 3 cut(s) 165, 1437, 1519
BstC8I GCNNGC 2 cut(s) 516, 1718
BstDEI CTNAG 8 cut(s) 640, 750, 765, 835, 1169, 1268, 1383, 1698
BstDSI CCRYGG 4 cut(s) 66, 123, 181, 1649
BstF5I GGATG 4 cut(s) 550, 558, 1108, 1704
BstKTI GATC 2 cut(s) 138, 1255
BstMAI GTCTC 2 cut(s) 789, 1422
BstMBI GATC 2 cut(s) 135, 1252
BstMWI GCNNNNNNNGC 1 cut(s) 1121
BstNSI RCATGY 1 cut(s) 518
BstSFI CTRYAG 2 cut(s) 87, 704
BstV1I GCAGC 4 cut(s) 73, 517, 757, 1633
BstV2I GAAGAC 1 cut(s) 1559
BstXI CCANNNNNNTGG 1 cut(s) 687
BsuI GTATCC 1 cut(s) 703
BsuRI GGCC 2 cut(s) 1449, 1647
BtgI CCRYGG 4 cut(s) 66, 123, 181, 1649
BtsCI GGATG 4 cut(s) 550, 558, 1108, 1704
BtsIMutI CAGTG 4 cut(s) 600, 1562, 1603, 1734
Cac8I GCNNGC 2 cut(s) 516, 1718
Cfr13I GGNCC 7 cut(s) 220, 341, 374, 843, 979, 1392, 1646
Csp6I GTAC 5 cut(s) 166, 296, 1438, 1520, 1702
CviQI GTAC 5 cut(s) 166, 296, 1438, 1520, 1702
DdeI CTNAG 8 cut(s) 640, 750, 765, 835, 1169, 1268, 1383, 1698
DpnI GATC 2 cut(s) 137, 1254
DpnII GATC 2 cut(s) 135, 1252
DraI TTTAAA 1 cut(s) 1504
Eco130I CCWWGG 5 cut(s) 66, 123, 181, 508, 917
Eco147I AGGCCT 1 cut(s) 1449
Eco47I GGWCC 6 cut(s) 220, 341, 374, 843, 979, 1392
Eco57I CTGAAG 1 cut(s) 390
Eco88I CYCGRG 1 cut(s) 299
EcoO109I RGGNCCY 2 cut(s) 341, 979
EcoRI GAATTC 1 cut(s) 359
EcoT14I CCWWGG 5 cut(s) 66, 123, 181, 508, 917
EcoT22I ATGCAT 2 cut(s) 558, 1548
ErhI CCWWGG 5 cut(s) 66, 123, 181, 508, 917
FaqI GGGAC 3 cut(s) 327, 661, 1738
FauI CCCGC 1 cut(s) 138
FblI GTMKAC 1 cut(s) 645
Fnu4HI GCNGC 4 cut(s) 87, 531, 746, 1622
FokI GGATG 4 cut(s) 557, 565, 1115, 1691
Fsp4HI GCNGC 4 cut(s) 87, 531, 746, 1622
FspBI CTAG 4 cut(s) 200, 407, 1116, 1625
GluI GCNGC 4 cut(s) 87, 531, 746, 1622
GsuI CTGGAG 2 cut(s) 969, 1184
HaeIII GGCC 2 cut(s) 1449, 1647
HincII GTYRAC 1 cut(s) 873
HindII GTYRAC 1 cut(s) 873
HindIII AAGCTT 1 cut(s) 1193
HinfI GANTC 5 cut(s) 92, 605, 833, 1216, 1270
HpaI GTTAAC 1 cut(s) 873
HphI GGTGA 2 cut(s) 36, 197
Hpy166II GTNNAC 9 cut(s) 168, 646, 843, 873, 1097, 1349, 1438, 1520, 1553
Hpy188III TCNNGA 9 cut(s) 290, 301, 356, 467, 609, 899, 986, 1064, 1306
Hpy8I GTNNAC 9 cut(s) 168, 646, 843, 873, 1097, 1349, 1438, 1520, 1553
HpyAV CCTTC 5 cut(s) 365, 470, 970, 1439, 1609
HpyCH4III ACNGT 4 cut(s) 1416, 1557, 1636, 1729
HpyCH4IV ACGT 1 cut(s) 330
HpyF10VI GCNNNNNNNGC 1 cut(s) 1121
HpyF3I CTNAG 8 cut(s) 640, 750, 765, 835, 1169, 1268, 1383, 1698
HpySE526I ACGT 1 cut(s) 330
KspAI GTTAAC 1 cut(s) 873
Kzo9I GATC 2 cut(s) 135, 1252
LmnI GCTCC 4 cut(s) 70, 742, 1165, 1618
Lsp1109I GCAGC 4 cut(s) 73, 517, 757, 1633
LweI GCATC 6 cut(s) 543, 565, 676, 899, 1015, 1533
MaeI CTAG 4 cut(s) 200, 407, 1116, 1625
MaeII ACGT 1 cut(s) 330
MaeIII GTNAC 5 cut(s) 326, 830, 927, 1271, 1654
MalI GATC 2 cut(s) 137, 1254
MboI GATC 2 cut(s) 135, 1252
MboII GAAGA 5 cut(s) 490, 500, 956, 1559, 1615
MfeI CAATTG 1 cut(s) 1533
MlyI GAGTC 3 cut(s) 599, 827, 1279
MmeI TCCRAC 1 cut(s) 216
Mph1103I ATGCAT 2 cut(s) 558, 1548
MroXI GAANNNNTTC 2 cut(s) 1025, 1403
MseI TTAA 8 cut(s) 249, 417, 440, 696, 872, 1286, 1343, 1503
MslI CAYNNNNRTG 2 cut(s) 454, 685
MspA1I CMGCKG 1 cut(s) 1697
MspCI CTTAAG 1 cut(s) 1285
MunI CAATTG 1 cut(s) 1533
MwoI GCNNNNNNNGC 1 cut(s) 1121
NcoI CCATGG 3 cut(s) 66, 123, 181
NdeII GATC 2 cut(s) 135, 1252
NlaIV GGNNCC 2 cut(s) 343, 981
NmuCI GTSAC 3 cut(s) 830, 927, 1271
NsiI ATGCAT 2 cut(s) 558, 1548
NspI RCATGY 1 cut(s) 518
PaeI GCATGC 1 cut(s) 518
PaeR7I CTCGAG 1 cut(s) 299
PceI AGGCCT 1 cut(s) 1449
PdmI GAANNNNTTC 2 cut(s) 1025, 1403
PfeI GAWTC 2 cut(s) 92, 1216
PkrI GCNGC 4 cut(s) 88, 532, 747, 1623
PleI GAGTC 3 cut(s) 599, 827, 1278
PpsI GAGTC 3 cut(s) 599, 827, 1278
PpuMI RGGWCCY 2 cut(s) 341, 979
PsiI TTATAA 1 cut(s) 1742
Psp5II RGGWCCY 2 cut(s) 341, 979
PspN4I GGNNCC 2 cut(s) 343, 981
PspPI GGNCC 7 cut(s) 220, 341, 374, 843, 979, 1392, 1646
PspPPI RGGWCCY 2 cut(s) 341, 979
PstI CTGCAG 2 cut(s) 91, 708
PvuII CAGCTG 1 cut(s) 1697
RsaI GTAC 5 cut(s) 167, 297, 1439, 1521, 1703
RsaNI GTAC 5 cut(s) 166, 296, 1438, 1520, 1702
RseI CAYNNNNRTG 2 cut(s) 454, 685
SaqAI TTAA 8 cut(s) 249, 417, 440, 696, 872, 1286, 1343, 1503
SatI GCNGC 4 cut(s) 87, 531, 746, 1622
Sau3AI GATC 2 cut(s) 135, 1252
Sau96I GGNCC 7 cut(s) 220, 341, 374, 843, 979, 1392, 1646
SchI GAGTC 3 cut(s) 599, 827, 1279
SfaNI GCATC 6 cut(s) 543, 565, 676, 899, 1015, 1533
SfcI CTRYAG 2 cut(s) 87, 704
Sfr274I CTCGAG 1 cut(s) 299
SinI GGWCC 6 cut(s) 220, 341, 374, 843, 979, 1392
SlaI CTCGAG 1 cut(s) 299
SmiMI CAYNNNNRTG 2 cut(s) 454, 685
SmlI CTYRAG 3 cut(s) 112, 299, 1285
SmoI CTYRAG 3 cut(s) 112, 299, 1285
SphI GCATGC 1 cut(s) 518
SseBI AGGCCT 1 cut(s) 1449
SsiI CCGC 1 cut(s) 145
SspI AATATT 1 cut(s) 156
SspMI CTAG 4 cut(s) 200, 407, 1116, 1625
StuI AGGCCT 1 cut(s) 1449
StyI CCWWGG 5 cut(s) 66, 123, 181, 508, 917
TaaI ACNGT 4 cut(s) 1416, 1557, 1636, 1729
TaiI ACGT 1 cut(s) 333
TaqI TCGA 2 cut(s) 300, 1151
TatI WGTACW 5 cut(s) 165, 295, 1437, 1519, 1701
TfiI GAWTC 2 cut(s) 92, 1216
Tru1I TTAA 8 cut(s) 249, 417, 440, 696, 872, 1286, 1343, 1503
Tru9I TTAA 8 cut(s) 249, 417, 440, 696, 872, 1286, 1343, 1503
TscAI CASTG 4 cut(s) 607, 1562, 1603, 1734
TseFI GTSAC 3 cut(s) 830, 927, 1271
TseI GCWGC 4 cut(s) 86, 530, 745, 1621
Tsp45I GTSAC 3 cut(s) 830, 927, 1271
TspRI CASTG 4 cut(s) 607, 1562, 1603, 1734
Vha464I CTTAAG 1 cut(s) 1285
VpaK11BI GGWCC 6 cut(s) 220, 341, 374, 843, 979, 1392
XapI RAATTY 4 cut(s) 14, 359, 1462, 1510
XceI RCATGY 1 cut(s) 518
XhoI CTCGAG 1 cut(s) 299
XmiI GTMKAC 1 cut(s) 645
XmnI GAANNNNTTC 2 cut(s) 1025, 1403
XspI CTAG 4 cut(s) 200, 407, 1116, 1625
Zsp2I ATGCAT 2 cut(s) 558, 1548
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.