RchiOBHm_Chr5g0072781

Belongs to the peptidase M16 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000891
Physical Location & Seq
Reverse (-)
78832881 .. 78841563
8683 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ34770

Sequence Viewer

Length: 3081 bp
ATGGCTCGCCGGACTTTCTCATCGGACGACGTCGTTTTGAAGTCTCCGAACGATAGGCGACTGTACCGATTGATCAAGCTCGAGAATGGCCTCACTGCATTGCTAGTTCACGATCCTGAGATTGGAGATTCCCAACTCCCCATGGGCTCTGAGGAAGCTGAGATGGAAGAAGAAGAAGGAATGGAGGAGGATGAGGATGAGGAAGATGACGATGAAGAAGACGAAGATAGTGAAGGAGAGGATGAAGAGATGGATGAAGATGATGAGGGAGAAGATGAAGATGGACTGAAGAAGAAAAAGGGAGGGGATTCTCAGACTAAGAAGGCAGCAGCGGCAATGTGTGTAGGAATAGGCAGCTTCTCTGATCCTCCTGAGGCACCGGGGCTTGCACACTTTCTAGAACACATGCTCTTTATGGGGAGTACAGAATTTCCAGATGAAAATGAGTATGACAGTTACTTGTCCAAGCATGGAGGGTGTTCAAATGCATACACGGAGGTAGAGCATACTTGCTACCATTTTGAAGTGAAACGAGAGTTTCTCAAGGGTGCCTTGACAAGATTTTCTCAGTTCTTTGTTTCACCCCTTGTAAAAATTGAAGCCATGGAGCGGGAGGTACAGGCTGTAGATTCAGAGTTTAACCAGGTTCTGCAGAACGATGCTTGCCGCCTTGAACAACTTCAATGCCATACAGCCTCACCTGGTCACCCATTTAATAGATTCGCATGGGGGAATAAAAAAAGCTTGAGCAATGCCATGGAAAAAGGGATCAACTTGCGGGAACAAATATTGAAATTGTACAGAGATTATTACCATGGTGGATTGATGAAGCTAGTTGTCATAGGAGGAGAATCTCTTGATGTACTTGAGAATTGGGTTTTGGAATTGTTTGGTAACGTCAAAAAAGGTCCCCAAGTAAATCTGGAATTCAAGGCGGAAGGTCCTATTTGGAAAGCTGGAAAAGTTTACAGGCTAGAGGCTGTTAAAGATGTTCATATACTCCACTTAACATGGACATTGCCATGCCTTCGTCAAGACTATTTGAAGAAATCAGAAGATTATTTATCTCATATCCTTGGGCATGAGGGCAGGGGAAGTTTGCATTCCTATTTCAAAGTTAAGGGGTGGGCAACATCTCTGGCTGCTGGTGTTGGGGATGACGGGATGCATCATTCTTCTGTGTCTTATGTCTTTCGCATGGACATTCATCTCACTGACTCTGGATTGGATAAGATTTTTGATATAATTGGCTTGGTCTACCAATACATAAAGTTATTGCATCAAGTGTCCCCACAAGAATGGATATTTAAGGAACTGCAGGATACTGGGAACATGGAATTTAGATTTGCAGAGGAGCAGCCTCAGGATGATTATGCCTCAGAACTTGCAGGAAATTTACTAATTTATGCAGCAGAACATGTTATTTATGGGTCCTACGCATACAAGATTTGGGATGAGGAATCGATCAAATATGTTCTTGATTTCCTTAGACCAGAAAACATGAGGATTGATGTTGTATCAAAGCCCTCATTTAAGTCAGAAGATTTCCAGTGCGAACCTTGGTTTGGGTCACATTATACTGAGGAAGATCTATCTCCATCTTTGATAGATTTGTGGAAGGAGCCTCCAGAAATTGATGTTTCATTGCATCTCCCAGAAAAGAATGAGTTCATTCCTACTGATTTTTCCATTCGTTCTGATGATCTTGATACTACAGATACAGCTTTTCCGAGATGTATACTGGATGAGCCATTGGTGAAGTTCTGGTACAAACTTGATAATACTTTTAAACTTCCGCGGGCAAATACTTATTTCCGCATCAATCCCAAGGGTGGATATGATAATGTGAAGAGTTGTGTCTTGACTGAACTATATGTATCCCTCCTCAAAGATGAGCTGAATGAGATTGTTTATCAGGCTAGTGTTGCCAAGCTGGAAACTTCGGTATCTGTATCGACTGACAAGCTGGAGCTAAAGGTCTACGGTTTCAATGACAAGCTTCCAGCTCTGTTGTCAAAGATTATGAAAACAACCAAAAGTTTCATGCCAACTTCTGATCGTTTTATGGTTATCAAAGAAGATATGGAGCGAAAGTTAAAGAACACCAATATGAAGCCTCTAAGTCACTCTACATACTTGAGAGTGCAAGTTCTGTTCCAGAGATTCTATGATGTTGATGAGAAGTTGCATGTTTTAAGTGGACTGTCTATTTCTGATATGAAGTCATTTATTCCCCAACTTTGGTCCCAGGTATACATTGAAGGCCTCTGTCATGGAAATTTGTCAGAAAAAGAAGCAATCAGCCTTTCAGATATATTTAAAACAAATTTTTGTGTACAACCACTTCCAATTGAACTGAGGCGCAGTGAACAGTGTATTTGTCTTCCTCCTTCTGCTAACCTCATTAGAGATGTCAGTGTGAAGAATAAGTCTGAAAAAAATTCGGTGATTGAGCTGTATTTTCAAATTGAGCGGGAATTGAGGATCGAGTCCCCCAGATTGAGAGCCCTGATAGATCTTTTCAATGAAATTGTGGAGGAACCACTTTTCAATCAGCTAAGGACGAAGGAGCAGCTTGGTTATACTGTTCATTGTGGCCCACGGGTTACTTGCAATATATTTGGATTTTATTTTTGCGTTCAGTCAGCTGAGTACAACCCAATCTACTTGCAAGGGAGAGTTGAGAACTTTATAAATAGTCTGGAGGAGTTGTTGCAAGGACTGGACGGTGACTCTTTTGAGGATTACAGAGCTGGGCTAATTGCAAAGCTGTTGGAGAAAGATCCATCCCTCCATTATGAAACCAATCGATATTGGTTACAGATTACTGATAAAATGTATATGTTTGACTATACAAAACAGGAGGCAGAACGACTCAGGAGCATTCAGAAGGAGGATGTCATCAACTGGTACAAAACATATTTGCAACAATCATCTCCCAAGTGTCGGAGACTTGCAATTCGTGTTTGGGGATGCAACACAGACTTGAAAGAGGCTGAAGCACGGCCGGAGTCTGTACTAGTCATTGAAGACCTTGCATCCTTTAAGATGTCATCTGAGTTCTATCCAAGCATTTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0003674 GO:0003824 GO:0004175 GO:0004222 GO:0005102 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005777 GO:0005782 GO:0005829 GO:0006508 GO:0006518 GO:0006605 GO:0006625 GO:0006807 GO:0006810 GO:0006886 GO:0006996 GO:0007031 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007169 GO:0007275 GO:0007568 GO:0008104 GO:0008144 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0008270 GO:0008286 GO:0008340 GO:0009056 GO:0009057 GO:0009719 GO:0009725 GO:0009893 GO:0009894 GO:0009896 GO:0009986 GO:0009987 GO:0010033 GO:0010243 GO:0010259 GO:0010604 GO:0010815 GO:0010992 GO:0015031 GO:0015833 GO:0016043 GO:0016787 GO:0017046 GO:0017076 GO:0017144 GO:0019222 GO:0019538 GO:0019725 GO:0022607 GO:0023052 GO:0030163 GO:0030554 GO:0031334 GO:0031907 GO:0031974 GO:0032459 GO:0032461 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0032868 GO:0032869 GO:0032870 GO:0033036 GO:0033218 GO:0033365 GO:0034613 GO:0034641 GO:0035639 GO:0036094 GO:0042176 GO:0042221 GO:0042277 GO:0042562 GO:0042579 GO:0042592 GO:0042737 GO:0042802 GO:0042803 GO:0042886 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043171 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043254 GO:0043434 GO:0043559 GO:0043574 GO:0043603 GO:0043933 GO:0044085 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044421 GO:0044422 GO:0044424 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0045732 GO:0046872 GO:0046907 GO:0046914 GO:0046983 GO:0048518 GO:0048522 GO:0048856 GO:0050435 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051259 GO:0051260 GO:0051603 GO:0051641 GO:0051649 GO:0051716 GO:0060255 GO:0065003 GO:0065007 GO:0065008 GO:0070011 GO:0070013 GO:0070727 GO:0070887 GO:0071310 GO:0071375 GO:0071417 GO:0071495 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072662 GO:0072663 GO:0080090 GO:0097159 GO:0097242 GO:0097367 GO:0140030 GO:0140035 GO:0140036 GO:0140096 GO:1901142 GO:1901143 GO:1901265 GO:1901363 GO:1901564 GO:1901565 GO:1901575 GO:1901652 GO:1901653 GO:1901698 GO:1901699 GO:1901700 GO:1901701
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_M16 Peptidase_M16_C Peptidase_M16_M

Protein Analysis

1026

Amino Acids

118.34

Weight (kDa)

4.87

Isoelectric Point (pI)

46.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M16 PF00675 104 - 234 1.6e-32 Insulinase (Peptidase family M16)
Peptidase_M16_C PF05193 260 - 440 1.9e-17 Peptidase M16 inactive domain
Peptidase_M16_M PF16187 447 - 730 1.4e-90 Middle or third domain of peptidase_M16
Peptidase_M16_C PF05193 735 - 920 4.5e-16 Peptidase M16 inactive domain
PqqF-like_C_4 PF22456 840 - 939 1.4e-24 PQQ synthase PqqF-like, C-terminal lobe domain 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2693
AatII GACGTC 1 cut(s) 33
AccB1I GGYRCC 2 cut(s) 376, 548
AccBSI CCGCTC 2 cut(s) 610, 2473
AccI GTMKAC 4 cut(s) 1257, 1738, 1980, 2253
AccII CGCG 1 cut(s) 1798
AciI CCGC 9 cut(s) 332, 610, 667, 778, 935, 1796, 1798, 1816, 2473
AclWI GGATC 5 cut(s) 107, 359, 776, 2492, 2777
AcoI YGGCCR 1 cut(s) 3005
AcsI RAATTY 7 cut(s) 428, 926, 1337, 1393, 2278, 2326, 2440
AcuI CTGAAG 2 cut(s) 308, 3018
AcyI GRCGYC 1 cut(s) 30
AfiI CCNNNNNNNGG 6 cut(s) 122, 609, 1565, 1832, 2241, 2946
AflIII ACRYGT 1 cut(s) 1417
AhlI ACTAGT 1 cut(s) 3019
AjnI CCWGG 3 cut(s) 642, 700, 2247
AjuI GAANNNNNNNTTGG 4 cut(s) 863, 895, 1548, 1580
Alw26I GTCTC 2 cut(s) 48, 2944
AlwI GGATC 5 cut(s) 107, 359, 776, 2492, 2777
AlwNI CAGNNNCTG 1 cut(s) 649
Ama87I CYCGRG 1 cut(s) 80
AoxI GGCC 4 cut(s) 88, 2263, 2596, 3005
ApeKI GCWGC 7 cut(s) 326, 329, 354, 1142, 1357, 1409, 2572
ApoI RAATTY 7 cut(s) 428, 926, 1337, 1393, 2278, 2326, 2440
ArsI GACNNNNNNTTYG 2 cut(s) 2943, 2975
Asp700I GAANNNNTTC 2 cut(s) 678, 1667
AspLEI GCGC 1 cut(s) 2364
AspS9I GGNCC 5 cut(s) 908, 941, 1431, 2244, 2597
AsuC2I CCSGG 1 cut(s) 381
AsuHPI GGTGA 6 cut(s) 573, 690, 698, 1768, 2458, 2741
AvaI CYCGRG 1 cut(s) 80
AvaII GGWCC 4 cut(s) 908, 941, 1431, 2244
AxyI CCTNAGG 2 cut(s) 372, 1362
BaeI ACNNNNGTAYC 2 cut(s) 1929, 1962
BanI GGYRCC 2 cut(s) 376, 548
BanII GRGCYC 2 cut(s) 149, 2509
BarI GAAGNNNNNNTAC 4 cut(s) 1751, 1783, 2328, 2360
BbsI GAAGAC 3 cut(s) 225, 2375, 3036
BbvI GCAGC 7 cut(s) 338, 341, 366, 1129, 1369, 1421, 2584
BccI CCATC 5 cut(s) 157, 244, 275, 1606, 2794
BceAI ACGGC 1 cut(s) 3020
BciT130I CCWGG 3 cut(s) 644, 702, 2249
BciVI GTATCC 2 cut(s) 1315, 1888
BclI TGATCA 1 cut(s) 72
BcnI CCSGG 1 cut(s) 381
BcoDI GTCTC 2 cut(s) 48, 2944
BcuI ACTAGT 1 cut(s) 3019
BfaI CTAG 6 cut(s) 104, 398, 833, 974, 1920, 3020
BfmI CTRYAG 4 cut(s) 624, 650, 1316, 1713
BfuI GTATCC 2 cut(s) 1315, 1888
BglII AGATCT 2 cut(s) 1588, 2515
BisI GCNGC 9 cut(s) 327, 330, 333, 355, 667, 1143, 1358, 1410, 2573
BlsI GCNGC 9 cut(s) 328, 331, 334, 356, 668, 1144, 1359, 1411, 2574
Bme1390I CCNGG 4 cut(s) 381, 644, 702, 2249
Bme18I GGWCC 4 cut(s) 908, 941, 1431, 2244
BmeT110I CYCGRG 1 cut(s) 80
BmgT120I GGNCC 5 cut(s) 908, 941, 1431, 2244, 2597
BmiI GGNNCC 7 cut(s) 378, 550, 910, 1432, 1623, 2246, 2541
BmrFI CCNGG 4 cut(s) 381, 644, 702, 2249
BmrI ACTGGG 1 cut(s) 1335
BmsI GCATC 8 cut(s) 649, 1155, 1177, 1288, 1657, 1827, 2963, 3047
BmuI ACTGGG 1 cut(s) 1335
BpiI GAAGAC 3 cut(s) 225, 2375, 3036
BplI GAGNNNNNCTC 2 cut(s) 525, 557
BpmI CTGGAG 3 cut(s) 1611, 1988, 2723
Bpu10I CCTNAGC 1 cut(s) 2558
BpuEI CTTGAG 4 cut(s) 527, 766, 887, 2158
BpuMI CCSGG 1 cut(s) 381
Bsa29I ATCGAT 2 cut(s) 1463, 2809
BsaHI GRCGYC 1 cut(s) 30
BsaXI ACNNNNNCTCC 4 cut(s) 2078, 2108, 2696, 2726
Bsc4I CCNNNNNNNGG 6 cut(s) 122, 609, 1565, 1832, 2241, 2946
Bse1I ACTGG 5 cut(s) 1330, 1549, 1746, 2727, 2912
Bse21I CCTNAGG 2 cut(s) 372, 1362
Bse3DI GCAATG 5 cut(s) 98, 342, 757, 1016, 1643
BseBI CCWGG 3 cut(s) 644, 702, 2249
BseCI ATCGAT 2 cut(s) 1463, 2809
BseLI CCNNNNNNNGG 6 cut(s) 122, 609, 1565, 1832, 2241, 2946
BseMI GCAATG 5 cut(s) 98, 342, 757, 1016, 1643
BseNI ACTGG 5 cut(s) 1330, 1549, 1746, 2727, 2912
BseRI GAGGAG 5 cut(s) 200, 861, 1367, 1874, 2720
BseX3I CGGCCG 1 cut(s) 3005
BseXI GCAGC 7 cut(s) 338, 341, 366, 1129, 1369, 1421, 2584
BseYI CCCAGC 1 cut(s) 2753
Bsh1236I CGCG 1 cut(s) 1798
Bsh1285I CGRYCG 1 cut(s) 3008
BshFI GGCC 4 cut(s) 90, 2265, 2598, 3007
BshNI GGYRCC 2 cut(s) 376, 548
BshVI ATCGAT 2 cut(s) 1463, 2809
BsiEI CGRYCG 1 cut(s) 3008
BsiHKCI CYCGRG 1 cut(s) 80
BsiSI CCGG 3 cut(s) 10, 380, 3008
BslFI GGGAC 4 cut(s) 894, 1273, 2230, 2476
BslI CCNNNNNNNGG 6 cut(s) 122, 609, 1565, 1832, 2241, 2946
BsmAI GTCTC 2 cut(s) 48, 2944
BsmFI GGGAC 4 cut(s) 894, 1273, 2230, 2476
BsmI GAATGC 2 cut(s) 1102, 2883
BsnI GGCC 4 cut(s) 90, 2265, 2598, 3007
BsoBI CYCGRG 1 cut(s) 80
Bsp1286I GDGCHC 2 cut(s) 149, 2509
Bsp1407I TGTACA 2 cut(s) 798, 2335
Bsp19I CCATGG 4 cut(s) 141, 603, 756, 814
BspACI CCGC 9 cut(s) 332, 610, 667, 778, 935, 1796, 1798, 1816, 2473
BspANI GGCC 4 cut(s) 90, 2265, 2598, 3007
BspDI ATCGAT 2 cut(s) 1463, 2809
BspFNI CGCG 1 cut(s) 1798
BspLI GGNNCC 7 cut(s) 378, 550, 910, 1432, 1623, 2246, 2541
BspMAI CTGCAG 2 cut(s) 654, 1320
BspPI GGATC 5 cut(s) 107, 359, 776, 2492, 2777
BspT107I GGYRCC 2 cut(s) 376, 548
BsrBI CCGCTC 2 cut(s) 610, 2473
BsrDI GCAATG 5 cut(s) 98, 342, 757, 1016, 1643
BsrGI TGTACA 2 cut(s) 798, 2335
BsrI ACTGG 5 cut(s) 1330, 1549, 1746, 2727, 2912
BssNAI GTATAC 2 cut(s) 1739, 2254
BssNI GRCGYC 1 cut(s) 30
BssT1I CCWWGG 7 cut(s) 141, 603, 756, 814, 1075, 1559, 1827
Bst1107I GTATAC 2 cut(s) 1739, 2254
Bst2UI CCWGG 3 cut(s) 644, 702, 2249
Bst4CI ACNGT 7 cut(s) 63, 455, 1985, 2205, 2373, 2587, 2729
Bst6I CTCTTC 2 cut(s) 240, 1844
BstACI GRCGYC 1 cut(s) 30
BstAUI TGTACA 2 cut(s) 798, 2335
BstC8I GCNNGC 4 cut(s) 7, 387, 664, 1800
BstDSI CCRYGG 6 cut(s) 141, 603, 756, 814, 1796, 2600
BstEII GGTNACC 1 cut(s) 704
BstFNI CGCG 1 cut(s) 1798
BstHHI GCGC 1 cut(s) 2364
BstMAI GTCTC 2 cut(s) 48, 2944
BstMCI CGRYCG 1 cut(s) 3008
BstMWI GCNNNNNNNGC 2 cut(s) 332, 1925
BstNI CCWGG 3 cut(s) 644, 702, 2249
BstNSI RCATGY 3 cut(s) 409, 1421, 2192
BstPI GGTNACC 1 cut(s) 704
BstSCI CCNGG 4 cut(s) 379, 642, 700, 2247
BstSFI CTRYAG 4 cut(s) 624, 650, 1316, 1713
BstUI CGCG 1 cut(s) 1798
BstV1I GCAGC 7 cut(s) 338, 341, 366, 1129, 1369, 1421, 2584
BstV2I GAAGAC 3 cut(s) 225, 2375, 3036
BstX2I RGATCY 3 cut(s) 1588, 2515, 2782
BstXI CCANNNNNNTGG 1 cut(s) 1299
BstYI RGATCY 3 cut(s) 1588, 2515, 2782
BstZ17I GTATAC 2 cut(s) 1739, 2254
BstZI CGGCCG 1 cut(s) 3005
Bsu15I ATCGAT 2 cut(s) 1463, 2809
Bsu36I CCTNAGG 2 cut(s) 372, 1362
BsuI GTATCC 2 cut(s) 1315, 1888
BsuRI GGCC 4 cut(s) 90, 2265, 2598, 3007
BsuTUI ATCGAT 2 cut(s) 1463, 2809
BtgI CCRYGG 6 cut(s) 141, 603, 756, 814, 1796, 2600
BtsI GCAGTG 2 cut(s) 93, 2371
BtsIMutI CAGTG 6 cut(s) 93, 1212, 1556, 2371, 2378, 2422
Cac8I GCNNGC 4 cut(s) 7, 387, 664, 1800
CaiI CAGNNNCTG 1 cut(s) 649
CfoI GCGC 1 cut(s) 2364
Cfr13I GGNCC 5 cut(s) 908, 941, 1431, 2244, 2597
Cfr42I CCGCGG 1 cut(s) 1799
ClaI ATCGAT 2 cut(s) 1463, 2809
CsiI ACCWGGT 2 cut(s) 642, 700
DraI TTTAAA 2 cut(s) 1789, 2320
EaeI YGGCCR 1 cut(s) 3005
EagI CGGCCG 1 cut(s) 3005
Eam1104I CTCTTC 2 cut(s) 240, 1844
EarI CTCTTC 2 cut(s) 240, 1844
EciI GGCGGA 1 cut(s) 950
EclXI CGGCCG 1 cut(s) 3005
Eco130I CCWWGG 7 cut(s) 141, 603, 756, 814, 1075, 1559, 1827
Eco147I AGGCCT 1 cut(s) 2265
Eco24I GRGCYC 2 cut(s) 149, 2509
Eco47I GGWCC 4 cut(s) 908, 941, 1431, 2244
Eco52I CGGCCG 1 cut(s) 3005
Eco57I CTGAAG 2 cut(s) 308, 3018
Eco81I CCTNAGG 2 cut(s) 372, 1362
Eco88I CYCGRG 1 cut(s) 80
Eco91I GGTNACC 1 cut(s) 704
EcoO109I RGGNCCY 3 cut(s) 908, 941, 1431
EcoO65I GGTNACC 1 cut(s) 704
EcoRI GAATTC 1 cut(s) 926
EcoRII CCWGG 3 cut(s) 642, 700, 2247
EcoT14I CCWWGG 7 cut(s) 141, 603, 756, 814, 1075, 1559, 1827
EcoT22I ATGCAT 2 cut(s) 490, 1170
EcoT38I GRGCYC 2 cut(s) 149, 2509
ErhI CCWWGG 7 cut(s) 141, 603, 756, 814, 1075, 1559, 1827
FalI AAGNNNNNCTT 2 cut(s) 536, 568
FaqI GGGAC 4 cut(s) 894, 1273, 2230, 2476
FauI CCCGC 4 cut(s) 603, 771, 1791, 2466
FbaI TGATCA 1 cut(s) 72
FblI GTMKAC 4 cut(s) 1257, 1738, 1980, 2253
Fnu4HI GCNGC 9 cut(s) 327, 330, 333, 355, 667, 1143, 1358, 1410, 2573
FriOI GRGCYC 2 cut(s) 149, 2509
Fsp4HI GCNGC 9 cut(s) 327, 330, 333, 355, 667, 1143, 1358, 1410, 2573
FspBI CTAG 6 cut(s) 104, 398, 833, 974, 1920, 3020
GlaI GCGC 1 cut(s) 2363
GluI GCNGC 9 cut(s) 327, 330, 333, 355, 667, 1143, 1358, 1410, 2573
GsaI CCCAGC 1 cut(s) 2757
GsuI CTGGAG 3 cut(s) 1611, 1988, 2723
HaeIII GGCC 4 cut(s) 90, 2265, 2598, 3007
HapII CCGG 3 cut(s) 10, 380, 3008
HhaI GCGC 1 cut(s) 2364
Hin1I GRCGYC 1 cut(s) 30
Hin6I GCGC 1 cut(s) 2362
HinP1I GCGC 1 cut(s) 2362
HindIII AAGCTT 2 cut(s) 742, 1997
HpaII CCGG 3 cut(s) 10, 380, 3008
HphI GGTGA 6 cut(s) 573, 690, 698, 1768, 2458, 2741
Hpy166II GTNNAC 9 cut(s) 109, 967, 1258, 1739, 1981, 2201, 2254, 2336, 2369
Hpy8I GTNNAC 9 cut(s) 109, 967, 1258, 1739, 1981, 2201, 2254, 2336, 2369
Hpy99I CGWCG 2 cut(s) 32, 35
HpyCH4III ACNGT 7 cut(s) 63, 455, 1985, 2205, 2373, 2587, 2729
HpyCH4IV ACGT 2 cut(s) 30, 897
HpyF10VI GCNNNNNNNGC 2 cut(s) 332, 1925
HpySE526I ACGT 2 cut(s) 30, 897
Hsp92I GRCGYC 1 cut(s) 30
HspAI GCGC 1 cut(s) 2362
Ksp22I TGATCA 1 cut(s) 72
KspI CCGCGG 1 cut(s) 1799
LmnI GCTCC 7 cut(s) 607, 1354, 1621, 1969, 2086, 2569, 2880
Lsp1109I GCAGC 7 cut(s) 338, 341, 366, 1129, 1369, 1421, 2584
LweI GCATC 8 cut(s) 649, 1155, 1177, 1288, 1657, 1827, 2963, 3047
MabI ACCWGGT 2 cut(s) 642, 700
MaeI CTAG 6 cut(s) 104, 398, 833, 974, 1920, 3020
MaeII ACGT 2 cut(s) 30, 897
MaeIII GTNAC 8 cut(s) 455, 704, 893, 1569, 2123, 2605, 2729, 2817
MbiI CCGCTC 2 cut(s) 610, 2473
MfeI CAATTG 1 cut(s) 2349
MflI RGATCY 3 cut(s) 1588, 2515, 2782
MhlI GDGCHC 2 cut(s) 149, 2509
MlyI GAGTC 5 cut(s) 1211, 2498, 2726, 2868, 3020
MmeI TCCRAC 2 cut(s) 2754, 2927
Mph1103I ATGCAT 2 cut(s) 490, 1170
MroXI GAANNNNTTC 2 cut(s) 678, 1667
MslI CAYNNNNRTG 3 cut(s) 1021, 1297, 2108
MspA1I CMGCKG 3 cut(s) 332, 1798, 2648
MspI CCGG 3 cut(s) 10, 380, 3008
MspR9I CCNGG 4 cut(s) 381, 644, 702, 2249
MunI CAATTG 1 cut(s) 2349
Mva1269I GAATGC 2 cut(s) 1102, 2883
MvaI CCWGG 3 cut(s) 644, 702, 2249
MvnI CGCG 1 cut(s) 1798
MwoI GCNNNNNNNGC 2 cut(s) 332, 1925
NciI CCSGG 1 cut(s) 381
NcoI CCATGG 4 cut(s) 141, 603, 756, 814
NlaIV GGNNCC 7 cut(s) 378, 550, 910, 1432, 1623, 2246, 2541
NmuCI GTSAC 4 cut(s) 704, 1569, 2123, 2729
NsiI ATGCAT 2 cut(s) 490, 1170
NspI RCATGY 3 cut(s) 409, 1421, 2192
PaeR7I CTCGAG 1 cut(s) 80
PceI AGGCCT 1 cut(s) 2265
PciI ACATGT 1 cut(s) 1417
PctI GAATGC 2 cut(s) 1102, 2883
PdmI GAANNNNTTC 2 cut(s) 678, 1667
PfeI GAWTC 7 cut(s) 128, 308, 629, 720, 851, 1460, 2163
PflFI GACNNNGTC 1 cut(s) 29
PkrI GCNGC 9 cut(s) 328, 331, 334, 356, 668, 1144, 1359, 1411, 2574
PleI GAGTC 5 cut(s) 1211, 2497, 2726, 2868, 3019
PpsI GAGTC 5 cut(s) 1211, 2497, 2726, 2868, 3019
PpuMI RGGWCCY 3 cut(s) 908, 941, 1431
PscI ACATGT 1 cut(s) 1417
PsiI TTATAA 1 cut(s) 2693
Psp5II RGGWCCY 3 cut(s) 908, 941, 1431
Psp6I CCWGG 3 cut(s) 642, 700, 2247
PspEI GGTNACC 1 cut(s) 704
PspFI CCCAGC 1 cut(s) 2753
PspGI CCWGG 3 cut(s) 642, 700, 2247
PspN4I GGNNCC 7 cut(s) 378, 550, 910, 1432, 1623, 2246, 2541
PspPI GGNCC 5 cut(s) 908, 941, 1431, 2244, 2597
PspPPI RGGWCCY 3 cut(s) 908, 941, 1431
PstI CTGCAG 2 cut(s) 654, 1320
PstNI CAGNNNCTG 1 cut(s) 649
PsuI RGATCY 3 cut(s) 1588, 2515, 2782
PsyI GACNNNGTC 1 cut(s) 29
PvuII CAGCTG 1 cut(s) 2648
RseI CAYNNNNRTG 3 cut(s) 1021, 1297, 2108
SacII CCGCGG 1 cut(s) 1799
SatI GCNGC 9 cut(s) 327, 330, 333, 355, 667, 1143, 1358, 1410, 2573
Sau96I GGNCC 5 cut(s) 908, 941, 1431, 2244, 2597
SchI GAGTC 5 cut(s) 1211, 2498, 2726, 2868, 3020
ScrFI CCNGG 4 cut(s) 381, 644, 702, 2249
SduI GDGCHC 2 cut(s) 149, 2509
SexAI ACCWGGT 2 cut(s) 642, 700
SfaNI GCATC 8 cut(s) 649, 1155, 1177, 1288, 1657, 1827, 2963, 3047
SfcI CTRYAG 4 cut(s) 624, 650, 1316, 1713
Sfr274I CTCGAG 1 cut(s) 80
Sfr303I CCGCGG 1 cut(s) 1799
SgrBI CCGCGG 1 cut(s) 1799
SinI GGWCC 4 cut(s) 908, 941, 1431, 2244
SlaI CTCGAG 1 cut(s) 80
SmiMI CAYNNNNRTG 3 cut(s) 1021, 1297, 2108
SmlI CTYRAG 5 cut(s) 80, 542, 745, 866, 2137
SmoI CTYRAG 5 cut(s) 80, 542, 745, 866, 2137
SpeI ACTAGT 1 cut(s) 3019
SseBI AGGCCT 1 cut(s) 2265
SsiI CCGC 9 cut(s) 332, 610, 667, 778, 935, 1796, 1798, 1816, 2473
SspI AATATT 1 cut(s) 789
SspMI CTAG 6 cut(s) 104, 398, 833, 974, 1920, 3020
StuI AGGCCT 1 cut(s) 2265
StyD4I CCNGG 4 cut(s) 379, 642, 700, 2247
StyI CCWWGG 7 cut(s) 141, 603, 756, 814, 1075, 1559, 1827
TaaI ACNGT 7 cut(s) 63, 455, 1985, 2205, 2373, 2587, 2729
TaiI ACGT 2 cut(s) 33, 900
TaqI TCGA 5 cut(s) 81, 1463, 1955, 2487, 2809
TatI WGTACW 6 cut(s) 422, 798, 862, 2335, 2652, 3016
TauI GCSGC 2 cut(s) 335, 669
TfiI GAWTC 7 cut(s) 128, 308, 629, 720, 851, 1460, 2163
TscAI CASTG 6 cut(s) 100, 1219, 1556, 2371, 2378, 2422
TseFI GTSAC 4 cut(s) 704, 1569, 2123, 2729
TseI GCWGC 7 cut(s) 326, 329, 354, 1142, 1357, 1409, 2572
Tsp45I GTSAC 4 cut(s) 704, 1569, 2123, 2729
TspGWI ACGGA 1 cut(s) 509
TspRI CASTG 6 cut(s) 100, 1219, 1556, 2371, 2378, 2422
Tth111I GACNNNGTC 1 cut(s) 29
VpaK11BI GGWCC 4 cut(s) 908, 941, 1431, 2244
XapI RAATTY 7 cut(s) 428, 926, 1337, 1393, 2278, 2326, 2440
XbaI TCTAGA 1 cut(s) 397
XceI RCATGY 3 cut(s) 409, 1421, 2192
XhoI CTCGAG 1 cut(s) 80
XmiI GTMKAC 4 cut(s) 1257, 1738, 1980, 2253
XmnI GAANNNNTTC 2 cut(s) 678, 1667
XspI CTAG 6 cut(s) 104, 398, 833, 974, 1920, 3020
ZraI GACGTC 1 cut(s) 31
Zsp2I ATGCAT 2 cut(s) 490, 1170
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.