AT1G65985

UPF0481 protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0000821
Physical Location & Seq
Reverse (-)
24562398 .. 24568966
6569 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G65985.2

Sequence Viewer

Length: 1371 bp
ATGGAAGTTCATATAGATTTGGATGAAAAAGTTCAGGAACCCTGCATTTATAGAGTACCAAATCGGATGCGAGTAGTGAAACCTGAAGCCTATACACCCAAGGTGGTCGTCATTGGCCCACTTCATCGTTCTCTCAAATCAACTGCTAAGGACGGCGCTGAAACAAGTAGCAATCCATGGCATCTTAAAAAAGATTATATCAAGATGGAAGTCACAAAGAAGACGTACTTCGAATCTTTCACCAAAAGAGTCGGGGAAGATACTATTGAGGAAATGAGGAAAACCATACAAACCGAAGAAAAAAATATTCGGAATTGCTATGAAGAATCAACTGAATGGATACCATCTGAATTATTCGTAGACCTTATCCTTCAGGATTCGATTTTCATTATGGAGTTAATTATCAGACTTTCTGGATTTGATCGAATACATTCAGATGACAGAAATTTAGACTTTGCAATCGTTATGAATGATTTACTCTTGCTCGAGAATCAGCTTCCCTATTTCATTTTGGATAGCTTGTTCAGTTTCAGTATGACGAAGTATTTGGATTCACACACCCTTGACAGTTTCATCTTGCGCTGTTTTGGCCTAAAGATCGAAGAGAAGAAAAACTTCATGCATTTGACAGACATGTACCGGTGTGTTTATAAAGAATCGCTTGACTACATTCCTCCACGAGAGTACTCACTGAATCGCGTTAAATGGTCTATCACAGAGTTGCGGAACGCTGAAAACCTATCAAATGCAGGAGTTAATTTCAAGTCATACGATTATTTAGATCTCTCAAAACAAGACGAGAAACTTGACATAAAGATTACGCGAGACAAGTCTGACTCTGAACTTACGAAATTACCAACAAATAAAAGAGAGAGGGTTGAAGGTGTTTCATTGCATGTGGAGTTAAAGAAAGGTTGCTTATCGATGCCGAGTTTCTGGGCTAATGATAGGACAGATATGATTCTAAGAAATGTAATTGCATTTGAGCAGTGCCAGCATGGCATAATACCCTTTACCTCGCACTACATACAATTCTTAAATGCCCTGATAGTTTCCGACAGAGACGTCGAGCTACTCAGCGAAAAAGGGGTGGTAATGAACAATATGGGTCGGCCAAGCTTGGTGGTGGACATGGTGAACAAGTTAAAGGTTGGTGTGTACGAAGGATACACCTCCCAATACTATGACATAGCCATAGATCTCAAAGCTCACTATAAATCTCGAAGAAAGAGGTGTTGGGCCACCCTCAGAAAAGTTTACTTCAGCGACTTGTGGACCGGCACAGCTACTTTAGCCGCCGTGCTTCTCTTGCTTTTGACTCTGGTTGGGACGGTGGCTTCTGTCATCCAAGCTTACGAAAGCTTCAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

456

Amino Acids

53.13

Weight (kDa)

6.41

Isoelectric Point (pI)

35.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 16 - 439 7.1e-88 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 651
AasI GACNNNNNNGTC 1 cut(s) 1064
AatII GACGTC 1 cut(s) 1068
AccI GTMKAC 1 cut(s) 360
AccII CGCG 2 cut(s) 699, 823
AciI CCGC 2 cut(s) 724, 1296
AcoI YGGCCR 1 cut(s) 1112
AcsI RAATTY 1 cut(s) 445
AcuI CTGAAG 3 cut(s) 105, 356, 1246
AcyI GRCGYC 1 cut(s) 1065
AfaI GTAC 5 cut(s) 57, 227, 638, 686, 1160
AflIII ACRYGT 1 cut(s) 633
AgeI ACCGGT 1 cut(s) 639
AgsI TTSAA 3 cut(s) 763, 881, 1366
AjuI GAANNNNNNNTTGG 2 cut(s) 1219, 1251
AloI GAACNNNNNNTCC 2 cut(s) 506, 538
AluBI AGCT 8 cut(s) 496, 519, 1072, 1119, 1208, 1286, 1352, 1362
AluI AGCT 8 cut(s) 496, 519, 1072, 1119, 1208, 1286, 1352, 1362
Alw26I GTCTC 2 cut(s) 819, 1056
Ama87I CYCGRG 1 cut(s) 485
AoxI GGCC 4 cut(s) 115, 589, 1112, 1239
ApoI RAATTY 1 cut(s) 445
ArsI GACNNNNNNTTYG 2 cut(s) 529, 561
AsiGI ACCGGT 1 cut(s) 639
Asp700I GAANNNNTTC 3 cut(s) 30, 430, 614
AspLEI GCGC 2 cut(s) 158, 582
AspS9I GGNCC 3 cut(s) 116, 1239, 1275
AsuHPI GGTGA 2 cut(s) 232, 1147
AsuII TTCGAA 1 cut(s) 231
AvaI CYCGRG 1 cut(s) 485
AvaII GGWCC 1 cut(s) 1275
BauI CACGAG 1 cut(s) 678
BbsI GAAGAC 1 cut(s) 227
BccI CCATC 2 cut(s) 199, 352
BceAI ACGGC 2 cut(s) 169, 1283
BciVI GTATCC 2 cut(s) 333, 1160
BcoDI GTCTC 2 cut(s) 819, 1056
BfoI RGCGCY 1 cut(s) 159
BfuI GTATCC 2 cut(s) 333, 1160
BglI GCCNNNNNGGC 1 cut(s) 999
BglII AGATCT 2 cut(s) 781, 1198
BisI GCNGC 1 cut(s) 1296
BlsI GCNGC 1 cut(s) 1297
BmcAI AGTACT 1 cut(s) 686
Bme18I GGWCC 1 cut(s) 1275
BmeT110I CYCGRG 1 cut(s) 485
BmgT120I GGNCC 3 cut(s) 116, 1239, 1275
BmiI GGNNCC 1 cut(s) 39
BmsI GCATC 3 cut(s) 57, 190, 915
BpiI GAAGAC 1 cut(s) 227
Bpu10I CCTNAGC 1 cut(s) 147
Bpu14I TTCGAA 1 cut(s) 231
Bsa29I ATCGAT 1 cut(s) 923
BsaHI GRCGYC 1 cut(s) 1065
BsaJI CCNNGG 2 cut(s) 99, 176
BsaWI WCCGGW 1 cut(s) 639
Bse118I RCCGGY 2 cut(s) 639, 1277
Bse3DI GCAATG 1 cut(s) 890
BseCI ATCGAT 1 cut(s) 923
BseDI CCNNGG 2 cut(s) 99, 176
BseGI GGATG 3 cut(s) 28, 72, 1344
BseMI GCAATG 1 cut(s) 890
BseMII CTCAG 2 cut(s) 1090, 1261
Bsh1236I CGCG 2 cut(s) 699, 823
BshFI GGCC 4 cut(s) 117, 591, 1114, 1241
BshTI ACCGGT 1 cut(s) 639
BshVI ATCGAT 1 cut(s) 923
BsiHKCI CYCGRG 1 cut(s) 485
BsiSI CCGG 2 cut(s) 640, 1278
BslFI GGGAC 1 cut(s) 1342
BsmAI GTCTC 2 cut(s) 819, 1056
BsmBI CGTCTC 1 cut(s) 1056
BsmFI GGGAC 1 cut(s) 1342
BsnI GGCC 4 cut(s) 117, 591, 1114, 1241
BsoBI CYCGRG 1 cut(s) 485
Bsp119I TTCGAA 1 cut(s) 231
Bsp143I GATC 4 cut(s) 421, 597, 781, 1198
Bsp19I CCATGG 1 cut(s) 176
BspACI CCGC 2 cut(s) 724, 1296
BspANI GGCC 4 cut(s) 117, 591, 1114, 1241
BspCNI CTCAG 2 cut(s) 1089, 1260
BspDI ATCGAT 1 cut(s) 923
BspFNI CGCG 2 cut(s) 699, 823
BspLI GGNNCC 1 cut(s) 39
BspT104I TTCGAA 1 cut(s) 231
BsrDI GCAATG 1 cut(s) 890
BsrFI RCCGGY 2 cut(s) 639, 1277
BssAI RCCGGY 2 cut(s) 639, 1277
BssECI CCNNGG 2 cut(s) 99, 176
BssMI GATC 4 cut(s) 421, 597, 781, 1198
BssNI GRCGYC 1 cut(s) 1065
BssSI CACGAG 1 cut(s) 678
BssT1I CCWWGG 2 cut(s) 99, 176
Bst2BI CACGAG 1 cut(s) 678
Bst4CI ACNGT 2 cut(s) 569, 1333
Bst6I CTCTTC 1 cut(s) 597
BstACI GRCGYC 1 cut(s) 1065
BstBI TTCGAA 1 cut(s) 231
BstC8I GCNNGC 1 cut(s) 995
BstDEI CTNAG 4 cut(s) 147, 965, 1076, 1247
BstDSI CCRYGG 1 cut(s) 176
BstF5I GGATG 3 cut(s) 28, 72, 1344
BstFNI CGCG 2 cut(s) 699, 823
BstH2I RGCGCY 1 cut(s) 159
BstHHI GCGC 2 cut(s) 158, 582
BstKTI GATC 4 cut(s) 424, 600, 784, 1201
BstMAI GTCTC 2 cut(s) 819, 1056
BstMBI GATC 4 cut(s) 421, 597, 781, 1198
BstMWI GCNNNNNNNGC 5 cut(s) 588, 994, 999, 1292, 1309
BstNSI RCATGY 2 cut(s) 637, 899
BstUI CGCG 2 cut(s) 699, 823
BstV2I GAAGAC 1 cut(s) 227
BstX2I RGATCY 2 cut(s) 781, 1198
BstYI RGATCY 2 cut(s) 781, 1198
Bsu15I ATCGAT 1 cut(s) 923
BsuI GTATCC 2 cut(s) 333, 1160
BsuRI GGCC 4 cut(s) 117, 591, 1114, 1241
BsuTUI ATCGAT 1 cut(s) 923
BtgI CCRYGG 1 cut(s) 176
BtsCI GGATG 3 cut(s) 28, 72, 1344
BtsI GCAGTG 1 cut(s) 995
BtsIMutI CAGTG 2 cut(s) 689, 995
Cac8I GCNNGC 1 cut(s) 995
CfoI GCGC 2 cut(s) 158, 582
Cfr10I RCCGGY 2 cut(s) 639, 1277
Cfr13I GGNCC 3 cut(s) 116, 1239, 1275
ClaI ATCGAT 1 cut(s) 923
Csp6I GTAC 5 cut(s) 56, 226, 637, 685, 1159
CspAI ACCGGT 1 cut(s) 639
CspCI CAANNNNNGTGG 2 cut(s) 1104, 1139
CviAII CATG 6 cut(s) 177, 619, 634, 896, 998, 1132
CviQI GTAC 5 cut(s) 56, 226, 637, 685, 1159
DdeI CTNAG 4 cut(s) 147, 965, 1076, 1247
DpnI GATC 4 cut(s) 423, 599, 783, 1200
DpnII GATC 4 cut(s) 421, 597, 781, 1198
DrdI GACNNNNNNGTC 1 cut(s) 1064
DseDI GACNNNNNNGTC 1 cut(s) 1064
EaeI YGGCCR 1 cut(s) 1112
Eam1104I CTCTTC 1 cut(s) 597
EarI CTCTTC 1 cut(s) 597
Eco130I CCWWGG 2 cut(s) 99, 176
Eco47I GGWCC 1 cut(s) 1275
Eco57I CTGAAG 3 cut(s) 105, 356, 1246
Eco88I CYCGRG 1 cut(s) 485
EcoT14I CCWWGG 2 cut(s) 99, 176
EcoT22I ATGCAT 1 cut(s) 624
ErhI CCWWGG 2 cut(s) 99, 176
Esp3I CGTCTC 1 cut(s) 1056
FaeI CATG 6 cut(s) 180, 622, 637, 899, 1001, 1135
FalI AAGNNNNNCTT 6 cut(s) 212, 244, 599, 631, 645, 677
FaqI GGGAC 1 cut(s) 1342
FatI CATG 6 cut(s) 176, 618, 633, 895, 997, 1131
FblI GTMKAC 1 cut(s) 360
Fnu4HI GCNGC 1 cut(s) 1296
FokI GGATG 3 cut(s) 35, 79, 1331
Fsp4HI GCNGC 1 cut(s) 1296
GlaI GCGC 2 cut(s) 157, 581
GluI GCNGC 1 cut(s) 1296
HaeII RGCGCY 1 cut(s) 159
HaeIII GGCC 4 cut(s) 117, 591, 1114, 1241
HapII CCGG 2 cut(s) 640, 1278
HhaI GCGC 2 cut(s) 158, 582
Hin1I GRCGYC 1 cut(s) 1065
Hin1II CATG 6 cut(s) 180, 622, 637, 899, 1001, 1135
Hin6I GCGC 2 cut(s) 156, 580
HinP1I GCGC 2 cut(s) 156, 580
HindIII AAGCTT 3 cut(s) 1117, 1350, 1360
HpaII CCGG 2 cut(s) 640, 1278
HphI GGTGA 2 cut(s) 232, 1147
Hpy166II GTNNAC 6 cut(s) 361, 1129, 1138, 1159, 1258, 1275
Hpy188I TCNGA 9 cut(s) 66, 312, 349, 407, 436, 835, 841, 1057, 1250
Hpy188III TCNNGA 6 cut(s) 35, 202, 374, 414, 487, 1221
Hpy8I GTNNAC 6 cut(s) 361, 1129, 1138, 1159, 1258, 1275
Hpy99I CGWCG 1 cut(s) 1070
HpyAV CCTTC 3 cut(s) 380, 875, 1157
HpyCH4III ACNGT 2 cut(s) 569, 1333
HpyCH4IV ACGT 2 cut(s) 224, 1065
HpyCH4V TGCA 6 cut(s) 45, 458, 622, 749, 895, 980
HpyF10VI GCNNNNNNNGC 5 cut(s) 588, 994, 999, 1292, 1309
HpyF3I CTNAG 4 cut(s) 147, 965, 1076, 1247
HpySE526I ACGT 2 cut(s) 224, 1065
Hsp92I GRCGYC 1 cut(s) 1065
Hsp92II CATG 6 cut(s) 180, 622, 637, 899, 1001, 1135
HspAI GCGC 2 cut(s) 156, 580
Kzo9I GATC 4 cut(s) 421, 597, 781, 1198
LweI GCATC 3 cut(s) 57, 190, 915
MaeII ACGT 2 cut(s) 224, 1065
MaeIII GTNAC 1 cut(s) 211
MalI GATC 4 cut(s) 423, 599, 783, 1200
MboI GATC 4 cut(s) 421, 597, 781, 1198
MboII GAAGA 7 cut(s) 232, 269, 308, 335, 614, 619, 1236
MflI RGATCY 2 cut(s) 781, 1198
MluCI AATT 8 cut(s) 313, 350, 399, 445, 757, 851, 975, 1031
MlyI GAGTC 3 cut(s) 258, 830, 1312
MmeI TCCRAC 1 cut(s) 1080
MnlI CCTC 8 cut(s) 262, 270, 684, 867, 1027, 1183, 1224, 1256
Mph1103I ATGCAT 1 cut(s) 624
MroXI GAANNNNTTC 3 cut(s) 30, 430, 614
MseI TTAA 7 cut(s) 186, 398, 702, 756, 905, 1037, 1145
MslI CAYNNNNRTG 1 cut(s) 435
MspI CCGG 2 cut(s) 640, 1278
MvnI CGCG 2 cut(s) 699, 823
MwoI GCNNNNNNNGC 5 cut(s) 588, 994, 999, 1292, 1309
NcoI CCATGG 1 cut(s) 176
NdeII GATC 4 cut(s) 421, 597, 781, 1198
NlaIII CATG 6 cut(s) 180, 622, 637, 899, 1001, 1135
NlaIV GGNNCC 1 cut(s) 39
NmeAIII GCCGAG 1 cut(s) 954
NmuCI GTSAC 1 cut(s) 211
NsiI ATGCAT 1 cut(s) 624
NspI RCATGY 2 cut(s) 637, 899
NspV TTCGAA 1 cut(s) 231
PaeR7I CTCGAG 1 cut(s) 485
PciI ACATGT 1 cut(s) 633
PdmI GAANNNNTTC 3 cut(s) 30, 430, 614
PfeI GAWTC 8 cut(s) 233, 326, 377, 490, 551, 656, 694, 961
PinAI ACCGGT 1 cut(s) 639
PkrI GCNGC 1 cut(s) 1297
PleI GAGTC 3 cut(s) 257, 830, 1312
PpsI GAGTC 3 cut(s) 257, 830, 1312
PscI ACATGT 1 cut(s) 633
PsiI TTATAA 1 cut(s) 651
PspN4I GGNNCC 1 cut(s) 39
PspPI GGNCC 3 cut(s) 116, 1239, 1275
PsuI RGATCY 2 cut(s) 781, 1198
RsaI GTAC 5 cut(s) 57, 227, 638, 686, 1160
RsaNI GTAC 5 cut(s) 56, 226, 637, 685, 1159
RseI CAYNNNNRTG 1 cut(s) 435
SaqAI TTAA 7 cut(s) 186, 398, 702, 756, 905, 1037, 1145
SatI GCNGC 1 cut(s) 1296
Sau3AI GATC 4 cut(s) 421, 597, 781, 1198
Sau96I GGNCC 3 cut(s) 116, 1239, 1275
ScaI AGTACT 1 cut(s) 686
SchI GAGTC 3 cut(s) 258, 830, 1312
SfaNI GCATC 3 cut(s) 57, 190, 915
Sfr274I CTCGAG 1 cut(s) 485
SfuI TTCGAA 1 cut(s) 231
SinI GGWCC 1 cut(s) 1275
SlaI CTCGAG 1 cut(s) 485
SmiMI CAYNNNNRTG 1 cut(s) 435
SmlI CTYRAG 1 cut(s) 485
SmoI CTYRAG 1 cut(s) 485
Sse9I AATT 8 cut(s) 313, 350, 399, 445, 757, 851, 975, 1031
SsiI CCGC 2 cut(s) 724, 1296
SspI AATATT 1 cut(s) 307
StyI CCWWGG 2 cut(s) 99, 176
TaaI ACNGT 2 cut(s) 569, 1333
TaiI ACGT 2 cut(s) 227, 1068
TaqI TCGA 8 cut(s) 231, 380, 424, 486, 600, 923, 1068, 1222
TasI AATT 8 cut(s) 313, 350, 399, 445, 757, 851, 975, 1031
TatI WGTACW 1 cut(s) 684
TauI GCSGC 1 cut(s) 1298
TfiI GAWTC 8 cut(s) 233, 326, 377, 490, 551, 656, 694, 961
Tru1I TTAA 7 cut(s) 186, 398, 702, 756, 905, 1037, 1145
Tru9I TTAA 7 cut(s) 186, 398, 702, 756, 905, 1037, 1145
TscAI CASTG 2 cut(s) 696, 995
TseFI GTSAC 1 cut(s) 211
Tsp45I GTSAC 1 cut(s) 211
TspRI CASTG 2 cut(s) 696, 995
VpaK11BI GGWCC 1 cut(s) 1275
XapI RAATTY 1 cut(s) 445
XceI RCATGY 2 cut(s) 637, 899
XhoI CTCGAG 1 cut(s) 485
XmiI GTMKAC 1 cut(s) 360
XmnI GAANNNNTTC 3 cut(s) 30, 430, 614
ZraI GACGTC 1 cut(s) 1066
ZrmI AGTACT 1 cut(s) 686
Zsp2I ATGCAT 1 cut(s) 624
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.