Rroxscaffold_5G00334990

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0000821
Physical Location & Seq
Reverse (-)
2429502 .. 2435543
6042 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00334990.1

Sequence Viewer

Length: 1296 bp
ATGGGGCGAGGGGGAAGGAATACCAGCAATTATGGTTTTAGGAGTGTCACATTCTTTGGCACTGATTATAGTTTCGTTTTCTCCAGGGAAATCAAGACTTCGGAAATGGTAGAACCAACTGTTCTGAGTGAACAAATAGAGAATGCACCCGTATTGTTCTCGCAGGAATGGTGTATCTATAGGGTTCCCAGCCAGCTCCGCAATGTAGATGAAGCAGCATACACTCCTCAAACACTTTCAATAGGGCCTTTCCACCATGGAAATCCAAAACTGAGGGACATGGTAGCCCATAAGAACAAATATTATGAGTTGTTTTGCAGACGTTCTGGGAAAAGTGAGGATGAACTAAGGAGCTTCATGATGGATCATCGAGAAACAGTTCTAAATTGTTATGCCGTTTTTAAGGCATACACTAAGGGAAAATCTGTCAGGAATGGATTTGAGCCGAAACACTTCACTGATTTGGTTAGACACTTTCTATGTCCATACAAGGAACTGTTGTGGGTAAAAAATTATGATCAGCCTGTCAAAATTAAATACCATGCAACAAAGCTGAAGGTTGCAGGAGTGAAGTTTGTGCCACTTAGAGAACCATTTGTTATAAAGGAAGACCCTGTGTTGACGTTTAACTCAGCATGTTTGGCACATAAGGAGTTGAAGCTTACAAGACTTTGGGCAGAGTATGAGGCAGGATGCATATTTCGAAACACCATGGCCTTGGAACAACTTATGTATCCAGAAAAGCCTTATATTTGCAGTTATTTTTCGCTGATGAATCAACTCATTAATTCTAAGGAAGATGTGGATGTTCTGATTGAGGATGGAGTAATCTTCAACTTGCTAGGCAGCAACAAAGCAGTGTTTGGTACCACCCTTGGTAATAACGAGACAGGTTTACTTCAAGGATCTTTGGAGAGGCAGTACAGCTATACTAGGATTCGTTGTCCTAATTTTCACAATTCTTCAATCCCTTCAGGCATTCGACTCGATTCGAAAAAGCAGTTCGAAAATAAGACAACAATTCGTACAAAAAAATTCCTAAAATCAAACAGATTAAATCGAGCTAAAAGCCAAACAGATTGGATAAAGCTAAAGAAACAAAAGCAAACCGCAGTGGAGGAACACAAAGAGCATCTCGATCTCGATCCATGTGAAAATCGCAGCAGATTTTCACTCTCTATGAATCGCAAATCTTCTACCACCAGCTCTAAATACAAATCGCAAGAGTTTCACACACCGAAATCGCCGGAAAGCTTCACAAACCAGAACCAGGGAGCTTCTCTCTCCTGTCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

431

Amino Acids

49.9

Weight (kDa)

9.34

Isoelectric Point (pI)

42.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 59 - 132 1.5e-17 Plant protein of unknown function
DUF247 PF03140 144 - 288 2e-24 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 602
Acc65I GGTACC 1 cut(s) 866
AccB1I GGYRCC 1 cut(s) 866
AciI CCGC 2 cut(s) 199, 1110
AclWI GGATC 3 cut(s) 372, 913, 1139
AcsI RAATTY 1 cut(s) 1034
AcuI CTGAAG 2 cut(s) 575, 957
AfaI GTAC 3 cut(s) 868, 923, 1027
AfiI CCNNNNNNNGG 1 cut(s) 1270
AgsI TTSAA 5 cut(s) 240, 658, 835, 902, 966
AjnI CCWGG 2 cut(s) 83, 1269
Alw26I GTCTC 1 cut(s) 881
AlwI GGATC 3 cut(s) 372, 913, 1139
AoxI GGCC 2 cut(s) 245, 714
ApeKI GCWGC 3 cut(s) 215, 846, 1161
ApoI RAATTY 1 cut(s) 1034
AseI ATTAAT 1 cut(s) 786
Asp700I GAANNNNTTC 2 cut(s) 378, 452
Asp718I GGTACC 1 cut(s) 866
AspS9I GGNCC 1 cut(s) 245
AsuII TTCGAA 3 cut(s) 703, 992, 1005
BaeI ACNNNNGTAYC 2 cut(s) 716, 749
BanI GGYRCC 1 cut(s) 866
BbsI GAAGAC 1 cut(s) 615
BbvI GCAGC 3 cut(s) 227, 858, 1173
BccI CCATC 2 cut(s) 355, 815
BceAI ACGGC 1 cut(s) 380
BcgI CGANNNNNNTGC 2 cut(s) 967, 1001
BciT130I CCWGG 2 cut(s) 85, 1271
BciVI GTATCC 1 cut(s) 744
BclI TGATCA 1 cut(s) 517
BcoDI GTCTC 1 cut(s) 881
BfaI CTAG 2 cut(s) 842, 933
BfmI CTRYAG 1 cut(s) 178
BfuI GTATCC 1 cut(s) 744
BisI GCNGC 3 cut(s) 216, 847, 1162
BlsI GCNGC 3 cut(s) 217, 848, 1163
Bme1390I CCNGG 2 cut(s) 85, 1271
BmgT120I GGNCC 1 cut(s) 245
BmiI GGNNCC 2 cut(s) 186, 868
BmrFI CCNGG 2 cut(s) 85, 1271
BmsI GCATC 2 cut(s) 683, 1141
BpiI GAAGAC 1 cut(s) 615
BplI GAGNNNNNCTC 1 cut(s) 1266
BpmI CTGGAG 1 cut(s) 67
Bpu14I TTCGAA 3 cut(s) 703, 992, 1005
BsaBI GATNNNNATC 1 cut(s) 1143
BsaJI CCNNGG 6 cut(s) 84, 256, 711, 717, 874, 1270
BsaXI ACNNNNNCTCC 2 cut(s) 905, 935
Bsc4I CCNNNNNNNGG 1 cut(s) 1270
Bse3DI GCAATG 1 cut(s) 208
Bse8I GATNNNNATC 1 cut(s) 1143
BseBI CCWGG 2 cut(s) 85, 1271
BseDI CCNNGG 6 cut(s) 84, 256, 711, 717, 874, 1270
BseGI GGATG 4 cut(s) 346, 698, 811, 826
BseJI GATNNNNATC 1 cut(s) 1143
BseLI CCNNNNNNNGG 1 cut(s) 1270
BseMI GCAATG 1 cut(s) 208
BseMII CTCAG 3 cut(s) 116, 263, 645
BseRI GAGGAG 1 cut(s) 216
BseXI GCAGC 3 cut(s) 227, 858, 1173
BseYI CCCAGC 1 cut(s) 188
BshFI GGCC 2 cut(s) 247, 716
BshNI GGYRCC 1 cut(s) 866
BsiSI CCGG 1 cut(s) 1247
BslFI GGGAC 1 cut(s) 290
BslI CCNNNNNNNGG 1 cut(s) 1270
BsmAI GTCTC 1 cut(s) 881
BsmFI GGGAC 1 cut(s) 290
BsmI GAATGC 2 cut(s) 148, 978
BsnI GGCC 2 cut(s) 247, 716
Bsp119I TTCGAA 3 cut(s) 703, 992, 1005
Bsp143I GATC 5 cut(s) 364, 517, 905, 1138, 1144
Bsp19I CCATGG 2 cut(s) 256, 711
BspACI CCGC 2 cut(s) 199, 1110
BspANI GGCC 2 cut(s) 247, 716
BspCNI CTCAG 3 cut(s) 117, 264, 644
BspHI TCATGA 1 cut(s) 357
BspLI GGNNCC 2 cut(s) 186, 868
BspPI GGATC 3 cut(s) 372, 913, 1139
BspT104I TTCGAA 3 cut(s) 703, 992, 1005
BspT107I GGYRCC 1 cut(s) 866
BsrDI GCAATG 1 cut(s) 208
BssECI CCNNGG 6 cut(s) 84, 256, 711, 717, 874, 1270
BssMI GATC 5 cut(s) 364, 517, 905, 1138, 1144
BssT1I CCWWGG 4 cut(s) 256, 711, 717, 874
Bst2UI CCWGG 2 cut(s) 85, 1271
Bst4CI ACNGT 3 cut(s) 121, 379, 498
BstBI TTCGAA 3 cut(s) 703, 992, 1005
BstC8I GCNNGC 1 cut(s) 194
BstDEI CTNAG 7 cut(s) 125, 272, 347, 414, 584, 631, 792
BstDSI CCRYGG 2 cut(s) 256, 711
BstF5I GGATG 4 cut(s) 346, 698, 811, 826
BstKTI GATC 5 cut(s) 367, 520, 908, 1141, 1147
BstMAI GTCTC 1 cut(s) 881
BstMBI GATC 5 cut(s) 364, 517, 905, 1138, 1144
BstMWI GCNNNNNNNGC 2 cut(s) 198, 641
BstNI CCWGG 2 cut(s) 85, 1271
BstNSI RCATGY 1 cut(s) 639
BstSCI CCNGG 2 cut(s) 83, 1269
BstSFI CTRYAG 1 cut(s) 178
BstV1I GCAGC 3 cut(s) 227, 858, 1173
BstV2I GAAGAC 1 cut(s) 615
BstX2I RGATCY 1 cut(s) 905
BstXI CCANNNNNNTGG 1 cut(s) 718
BstYI RGATCY 1 cut(s) 905
BsuI GTATCC 1 cut(s) 744
BsuRI GGCC 2 cut(s) 247, 716
BtgI CCRYGG 2 cut(s) 256, 711
BtsCI GGATG 4 cut(s) 346, 698, 811, 826
BtsI GCAGTG 2 cut(s) 864, 1119
BtsIMutI CAGTG 4 cut(s) 60, 456, 864, 1119
Cac8I GCNNGC 1 cut(s) 194
CciI TCATGA 1 cut(s) 357
Cfr13I GGNCC 1 cut(s) 245
Csp6I GTAC 3 cut(s) 867, 922, 1026
CviAII CATG 7 cut(s) 257, 280, 358, 542, 636, 712, 1149
CviQI GTAC 3 cut(s) 867, 922, 1026
DdeI CTNAG 7 cut(s) 125, 272, 347, 414, 584, 631, 792
DpnI GATC 5 cut(s) 366, 519, 907, 1140, 1146
DpnII GATC 5 cut(s) 364, 517, 905, 1138, 1144
Eco130I CCWWGG 4 cut(s) 256, 711, 717, 874
Eco57I CTGAAG 2 cut(s) 575, 957
EcoO109I RGGNCCY 1 cut(s) 245
EcoRII CCWGG 2 cut(s) 83, 1269
EcoT14I CCWWGG 4 cut(s) 256, 711, 717, 874
EcoT22I ATGCAT 1 cut(s) 698
ErhI CCWWGG 4 cut(s) 256, 711, 717, 874
FaeI CATG 7 cut(s) 260, 283, 361, 545, 639, 715, 1152
FaqI GGGAC 1 cut(s) 290
FatI CATG 7 cut(s) 256, 279, 357, 541, 635, 711, 1148
FbaI TGATCA 1 cut(s) 517
Fnu4HI GCNGC 3 cut(s) 216, 847, 1162
FokI GGATG 4 cut(s) 353, 705, 818, 833
Fsp4HI GCNGC 3 cut(s) 216, 847, 1162
FspBI CTAG 2 cut(s) 842, 933
GluI GCNGC 3 cut(s) 216, 847, 1162
GsaI CCCAGC 1 cut(s) 192
GsuI CTGGAG 1 cut(s) 67
HaeIII GGCC 2 cut(s) 247, 716
HapII CCGG 1 cut(s) 1247
Hin1II CATG 7 cut(s) 260, 283, 361, 545, 639, 715, 1152
HincII GTYRAC 1 cut(s) 621
HindII GTYRAC 1 cut(s) 621
HindIII AAGCTT 2 cut(s) 659, 1252
HinfI GANTC 5 cut(s) 775, 937, 984, 989, 1183
HpaII CCGG 1 cut(s) 1247
Hpy166II GTNNAC 3 cut(s) 131, 621, 896
Hpy188I TCNGA 3 cut(s) 103, 126, 813
Hpy188III TCNNGA 7 cut(s) 94, 358, 371, 430, 737, 1136, 1142
Hpy8I GTNNAC 3 cut(s) 131, 621, 896
HpyAV CCTTC 3 cut(s) 9, 550, 981
HpyCH4III ACNGT 3 cut(s) 121, 379, 498
HpyCH4IV ACGT 2 cut(s) 322, 623
HpyCH4V TGCA 6 cut(s) 146, 318, 545, 563, 696, 756
HpyF10VI GCNNNNNNNGC 2 cut(s) 198, 641
HpyF3I CTNAG 7 cut(s) 125, 272, 347, 414, 584, 631, 792
HpySE526I ACGT 2 cut(s) 322, 623
Hsp92II CATG 7 cut(s) 260, 283, 361, 545, 639, 715, 1152
KpnI GGTACC 1 cut(s) 870
Ksp22I TGATCA 1 cut(s) 517
Kzo9I GATC 5 cut(s) 364, 517, 905, 1138, 1144
LmnI GCTCC 3 cut(s) 201, 351, 1274
Lsp1109I GCAGC 3 cut(s) 227, 858, 1173
LweI GCATC 2 cut(s) 683, 1141
MaeI CTAG 2 cut(s) 842, 933
MaeII ACGT 2 cut(s) 322, 623
MaeIII GTNAC 1 cut(s) 46
MalI GATC 5 cut(s) 366, 519, 907, 1140, 1146
MboI GATC 5 cut(s) 364, 517, 905, 1138, 1144
MboII GAAGA 5 cut(s) 620, 809, 823, 954, 1185
MflI RGATCY 1 cut(s) 905
MluCI AATT 9 cut(s) 28, 385, 511, 531, 787, 949, 958, 1020, 1034
MlyI GAGTC 1 cut(s) 978
MnlI CCTC 7 cut(s) 237, 267, 331, 679, 811, 909, 1111
Mph1103I ATGCAT 1 cut(s) 698
MroXI GAANNNNTTC 2 cut(s) 378, 452
MseI TTAA 5 cut(s) 402, 534, 627, 786, 1055
MspI CCGG 1 cut(s) 1247
MspR9I CCNGG 2 cut(s) 85, 1271
Mva1269I GAATGC 2 cut(s) 148, 978
MvaI CCWGG 2 cut(s) 85, 1271
MwoI GCNNNNNNNGC 2 cut(s) 198, 641
NcoI CCATGG 2 cut(s) 256, 711
NdeII GATC 5 cut(s) 364, 517, 905, 1138, 1144
NlaIII CATG 7 cut(s) 260, 283, 361, 545, 639, 715, 1152
NlaIV GGNNCC 2 cut(s) 186, 868
NmuCI GTSAC 1 cut(s) 46
NsiI ATGCAT 1 cut(s) 698
NspI RCATGY 1 cut(s) 639
NspV TTCGAA 3 cut(s) 703, 992, 1005
PagI TCATGA 1 cut(s) 357
PctI GAATGC 2 cut(s) 148, 978
PdmI GAANNNNTTC 2 cut(s) 378, 452
PfeI GAWTC 4 cut(s) 775, 937, 989, 1183
PkrI GCNGC 3 cut(s) 217, 848, 1163
PleI GAGTC 1 cut(s) 978
PpsI GAGTC 1 cut(s) 978
PshBI ATTAAT 1 cut(s) 786
PsiI TTATAA 1 cut(s) 602
Psp6I CCWGG 2 cut(s) 83, 1269
PspFI CCCAGC 1 cut(s) 188
PspGI CCWGG 2 cut(s) 83, 1269
PspN4I GGNNCC 2 cut(s) 186, 868
PspPI GGNCC 1 cut(s) 245
PsuI RGATCY 1 cut(s) 905
RsaI GTAC 3 cut(s) 868, 923, 1027
RsaNI GTAC 3 cut(s) 867, 922, 1026
SaqAI TTAA 5 cut(s) 402, 534, 627, 786, 1055
SatI GCNGC 3 cut(s) 216, 847, 1162
Sau3AI GATC 5 cut(s) 364, 517, 905, 1138, 1144
Sau96I GGNCC 1 cut(s) 245
SchI GAGTC 1 cut(s) 978
ScrFI CCNGG 2 cut(s) 85, 1271
SfaNI GCATC 2 cut(s) 683, 1141
SfcI CTRYAG 1 cut(s) 178
SfuI TTCGAA 3 cut(s) 703, 992, 1005
Sse9I AATT 9 cut(s) 28, 385, 511, 531, 787, 949, 958, 1020, 1034
SsiI CCGC 2 cut(s) 199, 1110
SspI AATATT 1 cut(s) 302
SspMI CTAG 2 cut(s) 842, 933
StyD4I CCNGG 2 cut(s) 83, 1269
StyI CCWWGG 4 cut(s) 256, 711, 717, 874
TaaI ACNGT 3 cut(s) 121, 379, 498
TaiI ACGT 2 cut(s) 325, 626
TaqI TCGA 9 cut(s) 370, 703, 982, 987, 992, 1005, 1060, 1137, 1143
TasI AATT 9 cut(s) 28, 385, 511, 531, 787, 949, 958, 1020, 1034
TatI WGTACW 1 cut(s) 921
TfiI GAWTC 4 cut(s) 775, 937, 989, 1183
Tru1I TTAA 5 cut(s) 402, 534, 627, 786, 1055
Tru9I TTAA 5 cut(s) 402, 534, 627, 786, 1055
TscAI CASTG 4 cut(s) 67, 463, 864, 1119
TseFI GTSAC 1 cut(s) 46
TseI GCWGC 3 cut(s) 215, 846, 1161
Tsp45I GTSAC 1 cut(s) 46
TspDTI ATGAA 5 cut(s) 225, 346, 357, 788, 1196
TspRI CASTG 4 cut(s) 67, 463, 864, 1119
VspI ATTAAT 1 cut(s) 786
XapI RAATTY 1 cut(s) 1034
XceI RCATGY 1 cut(s) 639
XmnI GAANNNNTTC 2 cut(s) 378, 452
XspI CTAG 2 cut(s) 842, 933
Zsp2I ATGCAT 1 cut(s) 698
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.