pycom02g18260

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0000821
Physical Location & Seq
Reverse (-)
16549317 .. 16550985
1669 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g18260.4

Sequence Viewer

Length: 1239 bp
ATGAAATATCGACGGAAGAACCTAAGAAGGATAAAGACAACATCACAAATCGGGGAGAAAAATGATTGCGAACAAGATTTCCATGACCACGTACTAGTCTTGGACCAGCCAGACAAGTGGTGTATCTACAGGGTTCCCAGCAAACTTCGCAAAGTAAACGAAGCAGCATACACTCCTCAGTTACTATCAATCGGCCTTTTCCACTACGGCAAGCCGGAACTCAAGGACATGGAGACCCACAAAAATATGTACTACAAGAATTTTTCCGCGCGTTGTTCGAAGAAGGACTCTGCACTAAAAAAATTCATCAGGGCTCGTCAAGAACACATTCTTCGTTGTTATGCAGGGGCCATTGAGCTTGAAGAAGATCCTGTTGACATAATTGTGGTTGATGCCTGCTTCATCATTGAGCTCTTTTTGATAAACTCCTCTGAAACAAACAATCATGAAAACGATTACATTTTAAGATCACCGTGGCTGAGGAAAGCTGTAGAGCAGGAACTAATATTGCTTGAAAACCAGCTTCCGTATTCTCTTCTTCAAGAGCTATATGACTTTGCGGTGCCAGCCTCCAGTTCCCCTCATTCCAAAGAACGCTCAGGTGATCATTGTATTGATGTTTCAGAAGCTGAACCTCTCCTTGATAGTACAGATGTTCCAGAAGCTGAACCTGTCCCTGATGATCCCTTTCTCAAGCTTACATGCGAGTTCTTTAAAGATTACAGCAAGGGAAAATCCGTCAAGAATGGGGTAAAACCAAAACACTTCACTGATTTGGTAAGACATTTTCTCTGTCCCGAAGAAGAAATGACCTTTGTACATAATGCTTGTCCTATAAAAAATATATATGCTGTAAGGAAGCTGAAGGCAGCAGGGGTGAAGTTTACGCCACTTCATGAAGACGGATACTTTATCATAAAAGGAGACAAGCCTACTAAATGCAAGTTTAACTTGGCGTGTTTTCGAAATATGAACTTAAAGCTTGCACAATTTTGTGTCAAGGATGAGACAGAGTTCGTTATTCGAAACATCATGGCCTTGGAGCAGTTTTTGTACCCGGACAAGCCTTATATCTTGGCAAAGCTGCTTAACAAGCACTATGAGAATTTCTGGAACCATCATGTGGCGACCCTGAAACGAGTTTACTTCAAGGATCTTTGGACAAGCAGTTCGACTATACTGGGACTTTTCGTCCTGGTTTTCTCCATCATTGGAAGCATAAAGTCGCTCAAATCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

413

Amino Acids

47.9

Weight (kDa)

8.3

Isoelectric Point (pI)

48.23

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 562
AccB7I CCANNNNNTGG 1 cut(s) 1123
AccII CGCG 2 cut(s) 269, 271
AciI CCGC 2 cut(s) 267, 560
AclWI GGATC 3 cut(s) 362, 677, 1161
AcsI RAATTY 3 cut(s) 259, 302, 1105
AcuI CTGAAG 1 cut(s) 884
AfaI GTAC 5 cut(s) 93, 251, 649, 819, 1055
AfiI CCNNNNNNNGG 1 cut(s) 1123
AgsI TTSAA 4 cut(s) 362, 515, 542, 1150
AhdI GACNNNNNGTC 1 cut(s) 1190
AhlI ACTAGT 1 cut(s) 94
AjnI CCWGG 1 cut(s) 1194
AloI GAACNNNNNNTCC 4 cut(s) 63, 95, 1153, 1185
Alw21I GWGCWC 1 cut(s) 414
Alw26I GTCTC 3 cut(s) 227, 918, 1001
AlwI GGATC 3 cut(s) 362, 677, 1161
AlwNI CAGNNNCTG 2 cut(s) 629, 665
AoxI GGCC 3 cut(s) 193, 348, 1035
ApeKI GCWGC 3 cut(s) 164, 869, 1084
ApoI RAATTY 3 cut(s) 259, 302, 1105
ArsI GACNNNNNNTTYG 2 cut(s) 1153, 1185
Asp700I GAANNNNTTC 1 cut(s) 327
AspLEI GCGC 1 cut(s) 271
AspS9I GGNCC 2 cut(s) 103, 348
AsuC2I CCSGG 1 cut(s) 1058
AsuHPI GGTGA 3 cut(s) 462, 614, 889
AsuII TTCGAA 3 cut(s) 278, 964, 1024
AvaII GGWCC 1 cut(s) 103
BanI GGYRCC 1 cut(s) 562
BanII GRGCYC 2 cut(s) 316, 414
BbsI GAAGAC 1 cut(s) 906
Bbv12I GWGCWC 1 cut(s) 414
BbvCI CCTCAGC 1 cut(s) 479
BbvI GCAGC 3 cut(s) 176, 881, 1071
BccI CCATC 2 cut(s) 1125, 1214
BceAI ACGGC 1 cut(s) 223
BciT130I CCWGG 1 cut(s) 1196
BciVI GTATCC 1 cut(s) 899
BclI TGATCA 1 cut(s) 604
BcnI CCSGG 1 cut(s) 1058
BcoDI GTCTC 3 cut(s) 227, 918, 1001
BcuI ACTAGT 1 cut(s) 94
BfaI CTAG 1 cut(s) 95
BfmI CTRYAG 2 cut(s) 127, 489
BfuI GTATCC 1 cut(s) 899
BisI GCNGC 3 cut(s) 165, 870, 1085
BlsI GCNGC 3 cut(s) 166, 871, 1086
Bme1390I CCNGG 2 cut(s) 1058, 1196
Bme18I GGWCC 1 cut(s) 103
BmeRI GACNNNNNGTC 1 cut(s) 1190
BmgT120I GGNCC 2 cut(s) 103, 348
BmiI GGNNCC 4 cut(s) 135, 349, 564, 1115
BmrFI CCNGG 2 cut(s) 1058, 1196
BmrI ACTGGG 1 cut(s) 1190
BmsI GCATC 1 cut(s) 382
BmuI ACTGGG 1 cut(s) 1190
BpiI GAAGAC 1 cut(s) 906
BpmI CTGGAG 1 cut(s) 556
Bpu10I CCTNAGC 2 cut(s) 479, 598
Bpu14I TTCGAA 3 cut(s) 278, 964, 1024
BpuEI CTTGAG 2 cut(s) 206, 677
BpuMI CCSGG 1 cut(s) 1058
BsaAI YACGTR 1 cut(s) 91
BsaI GGTCTC 1 cut(s) 227
BsaJI CCNNGG 2 cut(s) 473, 1038
Bsc4I CCNNNNNNNGG 1 cut(s) 1123
Bse1I ACTGG 2 cut(s) 573, 1185
BseBI CCWGG 1 cut(s) 1196
BseDI CCNNGG 2 cut(s) 473, 1038
BseGI GGATG 1 cut(s) 1009
BseLI CCNNNNNNNGG 1 cut(s) 1123
BseMII CTCAG 3 cut(s) 191, 470, 612
BseNI ACTGG 2 cut(s) 573, 1185
BseRI GAGGAG 2 cut(s) 165, 418
BseXI GCAGC 3 cut(s) 176, 881, 1071
BseYI CCCAGC 1 cut(s) 137
BsgI GTGCAG 1 cut(s) 276
Bsh1236I CGCG 2 cut(s) 269, 271
BshFI GGCC 3 cut(s) 195, 350, 1037
BshNI GGYRCC 1 cut(s) 562
BsiHKAI GWGCWC 1 cut(s) 414
BsiSI CCGG 2 cut(s) 215, 1058
BslFI GGGAC 3 cut(s) 659, 780, 1197
BslI CCNNNNNNNGG 1 cut(s) 1123
BsmAI GTCTC 3 cut(s) 227, 918, 1001
BsmFI GGGAC 3 cut(s) 659, 780, 1197
BsnI GGCC 3 cut(s) 195, 350, 1037
Bso31I GGTCTC 1 cut(s) 227
Bsp119I TTCGAA 3 cut(s) 278, 964, 1024
Bsp1286I GDGCHC 2 cut(s) 316, 414
Bsp1407I TGTACA 1 cut(s) 817
Bsp143I GATC 5 cut(s) 367, 467, 604, 682, 1153
BspACI CCGC 2 cut(s) 267, 560
BspANI GGCC 3 cut(s) 195, 350, 1037
BspCNI CTCAG 3 cut(s) 190, 471, 611
BspFNI CGCG 2 cut(s) 269, 271
BspHI TCATGA 2 cut(s) 445, 895
BspLI GGNNCC 4 cut(s) 135, 349, 564, 1115
BspPI GGATC 3 cut(s) 362, 677, 1161
BspT104I TTCGAA 3 cut(s) 278, 964, 1024
BspT107I GGYRCC 1 cut(s) 562
BspTNI GGTCTC 1 cut(s) 227
BsrGI TGTACA 1 cut(s) 817
BsrI ACTGG 2 cut(s) 573, 1185
BssECI CCNNGG 2 cut(s) 473, 1038
BssMI GATC 5 cut(s) 367, 467, 604, 682, 1153
BssT1I CCWWGG 1 cut(s) 1038
Bst2UI CCWGG 1 cut(s) 1196
Bst4CI ACNGT 1 cut(s) 474
Bst6I CTCTTC 1 cut(s) 540
BstAUI TGTACA 1 cut(s) 817
BstBAI YACGTR 1 cut(s) 91
BstBI TTCGAA 3 cut(s) 278, 964, 1024
BstC8I GCNNGC 4 cut(s) 212, 397, 567, 984
BstDEI CTNAG 4 cut(s) 23, 177, 479, 598
BstDSI CCRYGG 1 cut(s) 473
BstF5I GGATG 1 cut(s) 1009
BstFNI CGCG 2 cut(s) 269, 271
BstHHI GCGC 1 cut(s) 271
BstKTI GATC 5 cut(s) 370, 470, 607, 685, 1156
BstMAI GTCTC 3 cut(s) 227, 918, 1001
BstMBI GATC 5 cut(s) 367, 467, 604, 682, 1153
BstMWI GCNNNNNNNGC 3 cut(s) 147, 566, 1093
BstNI CCWGG 1 cut(s) 1196
BstNSI RCATGY 1 cut(s) 705
BstSCI CCNGG 2 cut(s) 1056, 1194
BstSFI CTRYAG 2 cut(s) 127, 489
BstUI CGCG 2 cut(s) 269, 271
BstV1I GCAGC 3 cut(s) 176, 881, 1071
BstV2I GAAGAC 1 cut(s) 906
BstX2I RGATCY 2 cut(s) 367, 1153
BstXI CCANNNNNNTGG 1 cut(s) 117
BstYI RGATCY 2 cut(s) 367, 1153
BsuI GTATCC 1 cut(s) 899
BsuRI GGCC 3 cut(s) 195, 350, 1037
BtgI CCRYGG 1 cut(s) 473
BtsCI GGATG 1 cut(s) 1009
BtsIMutI CAGTG 1 cut(s) 768
Cac8I GCNNGC 4 cut(s) 212, 397, 567, 984
CaiI CAGNNNCTG 2 cut(s) 629, 665
CciI TCATGA 2 cut(s) 445, 895
CfoI GCGC 1 cut(s) 271
Cfr13I GGNCC 2 cut(s) 103, 348
Csp6I GTAC 5 cut(s) 92, 250, 648, 818, 1054
CviAII CATG 7 cut(s) 83, 229, 446, 702, 896, 1033, 1121
CviQI GTAC 5 cut(s) 92, 250, 648, 818, 1054
DdeI CTNAG 4 cut(s) 23, 177, 479, 598
DpnI GATC 5 cut(s) 369, 469, 606, 684, 1155
DpnII GATC 5 cut(s) 367, 467, 604, 682, 1153
DraI TTTAAA 1 cut(s) 715
DriI GACNNNNNGTC 1 cut(s) 1190
Eam1104I CTCTTC 1 cut(s) 540
Eam1105I GACNNNNNGTC 1 cut(s) 1190
EarI CTCTTC 1 cut(s) 540
Ecl136II GAGCTC 1 cut(s) 412
Eco130I CCWWGG 1 cut(s) 1038
Eco24I GRGCYC 2 cut(s) 316, 414
Eco31I GGTCTC 1 cut(s) 227
Eco47I GGWCC 1 cut(s) 103
Eco53kI GAGCTC 1 cut(s) 412
Eco57I CTGAAG 1 cut(s) 884
EcoICRI GAGCTC 1 cut(s) 412
EcoRII CCWGG 1 cut(s) 1194
EcoT14I CCWWGG 1 cut(s) 1038
EcoT38I GRGCYC 2 cut(s) 316, 414
ErhI CCWWGG 1 cut(s) 1038
FaeI CATG 7 cut(s) 86, 232, 449, 705, 899, 1036, 1124
FalI AAGNNNNNCTT 2 cut(s) 935, 967
FaqI GGGAC 3 cut(s) 659, 780, 1197
FatI CATG 7 cut(s) 82, 228, 445, 701, 895, 1032, 1120
FbaI TGATCA 1 cut(s) 604
Fnu4HI GCNGC 3 cut(s) 165, 870, 1085
FokI GGATG 1 cut(s) 1016
FriOI GRGCYC 2 cut(s) 316, 414
Fsp4HI GCNGC 3 cut(s) 165, 870, 1085
FspBI CTAG 1 cut(s) 95
GlaI GCGC 1 cut(s) 270
GluI GCNGC 3 cut(s) 165, 870, 1085
GsaI CCCAGC 1 cut(s) 141
GsuI CTGGAG 1 cut(s) 556
HaeIII GGCC 3 cut(s) 195, 350, 1037
HapII CCGG 2 cut(s) 215, 1058
HhaI GCGC 1 cut(s) 271
Hin1II CATG 7 cut(s) 86, 232, 449, 705, 899, 1036, 1124
Hin6I GCGC 1 cut(s) 269
HinP1I GCGC 1 cut(s) 269
HincII GTYRAC 1 cut(s) 376
HindII GTYRAC 1 cut(s) 376
HindIII AAGCTT 2 cut(s) 695, 980
HinfI GANTC 1 cut(s) 287
HpaII CCGG 2 cut(s) 215, 1058
HphI GGTGA 3 cut(s) 462, 614, 889
Hpy166II GTNNAC 4 cut(s) 157, 376, 885, 1144
Hpy188I TCNGA 2 cut(s) 433, 625
Hpy188III TCNNGA 8 cut(s) 320, 446, 542, 659, 742, 797, 896, 1111
Hpy8I GTNNAC 4 cut(s) 157, 376, 885, 1144
Hpy99I CGWCG 1 cut(s) 15
HpyAV CCTTC 3 cut(s) 21, 277, 859
HpyCH4III ACNGT 1 cut(s) 474
HpyCH4IV ACGT 1 cut(s) 90
HpyCH4V TGCA 4 cut(s) 293, 344, 942, 986
HpyF10VI GCNNNNNNNGC 3 cut(s) 147, 566, 1093
HpyF3I CTNAG 4 cut(s) 23, 177, 479, 598
HpySE526I ACGT 1 cut(s) 90
Hsp92II CATG 7 cut(s) 86, 232, 449, 705, 899, 1036, 1124
HspAI GCGC 1 cut(s) 269
Ksp22I TGATCA 1 cut(s) 604
Kzo9I GATC 5 cut(s) 367, 467, 604, 682, 1153
LmnI GCTCC 1 cut(s) 1042
Lsp1109I GCAGC 3 cut(s) 176, 881, 1071
LweI GCATC 1 cut(s) 382
MaeI CTAG 1 cut(s) 95
MaeII ACGT 1 cut(s) 90
MaeIII GTNAC 1 cut(s) 180
MalI GATC 5 cut(s) 369, 469, 606, 684, 1155
MboI GATC 5 cut(s) 367, 467, 604, 682, 1153
MflI RGATCY 2 cut(s) 367, 1153
MhlI GDGCHC 2 cut(s) 316, 414
MluCI AATT 5 cut(s) 259, 302, 381, 989, 1105
MlyI GAGTC 1 cut(s) 281
MnlI CCTC 6 cut(s) 186, 439, 474, 580, 591, 645
MroXI GAANNNNTTC 1 cut(s) 327
MseI TTAA 5 cut(s) 464, 714, 948, 977, 1089
MslI CAYNNNNRTG 1 cut(s) 383
MspI CCGG 2 cut(s) 215, 1058
MspR9I CCNGG 2 cut(s) 1058, 1196
MvaI CCWGG 1 cut(s) 1196
MvnI CGCG 2 cut(s) 269, 271
MwoI GCNNNNNNNGC 3 cut(s) 147, 566, 1093
NciI CCSGG 1 cut(s) 1058
NdeII GATC 5 cut(s) 367, 467, 604, 682, 1153
NlaIII CATG 7 cut(s) 86, 232, 449, 705, 899, 1036, 1124
NlaIV GGNNCC 4 cut(s) 135, 349, 564, 1115
NspI RCATGY 1 cut(s) 705
NspV TTCGAA 3 cut(s) 278, 964, 1024
PagI TCATGA 2 cut(s) 445, 895
PdmI GAANNNNTTC 1 cut(s) 327
PflMI CCANNNNNTGG 1 cut(s) 1123
PkrI GCNGC 3 cut(s) 166, 871, 1086
PleI GAGTC 1 cut(s) 281
PpsI GAGTC 1 cut(s) 281
Ppu21I YACGTR 1 cut(s) 91
Psp124BI GAGCTC 1 cut(s) 414
Psp6I CCWGG 1 cut(s) 1194
PspFI CCCAGC 1 cut(s) 137
PspGI CCWGG 1 cut(s) 1194
PspN4I GGNNCC 4 cut(s) 135, 349, 564, 1115
PspPI GGNCC 2 cut(s) 103, 348
PstNI CAGNNNCTG 2 cut(s) 629, 665
PsuI RGATCY 2 cut(s) 367, 1153
RsaI GTAC 5 cut(s) 93, 251, 649, 819, 1055
RsaNI GTAC 5 cut(s) 92, 250, 648, 818, 1054
RseI CAYNNNNRTG 1 cut(s) 383
SacI GAGCTC 1 cut(s) 414
SaqAI TTAA 5 cut(s) 464, 714, 948, 977, 1089
SatI GCNGC 3 cut(s) 165, 870, 1085
Sau3AI GATC 5 cut(s) 367, 467, 604, 682, 1153
Sau96I GGNCC 2 cut(s) 103, 348
SchI GAGTC 1 cut(s) 281
ScrFI CCNGG 2 cut(s) 1058, 1196
SduI GDGCHC 2 cut(s) 316, 414
SfaNI GCATC 1 cut(s) 382
SfcI CTRYAG 2 cut(s) 127, 489
SfuI TTCGAA 3 cut(s) 278, 964, 1024
SinI GGWCC 1 cut(s) 103
SmiMI CAYNNNNRTG 1 cut(s) 383
SmlI CTYRAG 2 cut(s) 221, 692
SmoI CTYRAG 2 cut(s) 221, 692
SpeI ACTAGT 1 cut(s) 94
Sse9I AATT 5 cut(s) 259, 302, 381, 989, 1105
SsiI CCGC 2 cut(s) 267, 560
SspI AATATT 1 cut(s) 507
SspMI CTAG 1 cut(s) 95
SstI GAGCTC 1 cut(s) 414
StyD4I CCNGG 2 cut(s) 1056, 1194
StyI CCWWGG 1 cut(s) 1038
TaaI ACNGT 1 cut(s) 474
TaiI ACGT 1 cut(s) 93
TaqI TCGA 5 cut(s) 10, 278, 964, 1024, 1172
TasI AATT 5 cut(s) 259, 302, 381, 989, 1105
TatI WGTACW 3 cut(s) 249, 647, 817
Tru1I TTAA 5 cut(s) 464, 714, 948, 977, 1089
Tru9I TTAA 5 cut(s) 464, 714, 948, 977, 1089
TscAI CASTG 1 cut(s) 775
TseI GCWGC 3 cut(s) 164, 869, 1084
TspDTI ATGAA 7 cut(s) 17, 295, 391, 462, 884, 912, 986
TspGWI ACGGA 4 cut(s) 28, 516, 727, 918
TspRI CASTG 1 cut(s) 775
Van91I CCANNNNNTGG 1 cut(s) 1123
VpaK11BI GGWCC 1 cut(s) 103
XapI RAATTY 3 cut(s) 259, 302, 1105
XceI RCATGY 1 cut(s) 705
XmnI GAANNNNTTC 1 cut(s) 327
XspI CTAG 1 cut(s) 95
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.