Rorug03G0318400

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0000821
Physical Location & Seq
Forward (+)
34404524 .. 34405636
1113 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0318400.1

Sequence Viewer

Length: 717 bp
ATGATGATGCTTCCACATGTTCTTCTGGCAAAGATGCTTTTGGGTTATAACGCGTGGAAGTATACAGATGTGGAGGCAATGAAAGAAAAGTTTACAAAGCTGCTATTGGGTGAAGATATTACAGGAGGGTACAAGGGCCTCAGCACTGCTTTAGCATTGTCAAATGCCATCACAAATCTAGCAGCAACGGTTTTTGGAGAGCTGTGGAAACTGGAACCACTATCTGAAGAGAGGAAAACAAAATGGCAAAGAGAAATGGACTGGTTACTCTCCCCTACCAACTATATGGTTGAGCTAGTTCCTTCCAGGCAAAATGGTGCTAATGGCCGGACAGTAGAGATTATGACTCCGAAAGCTCGTGCAGACATACACATCAATCTTCCAGCACTTAAGAAGTTGGACTCGATGCTTATTGAAACACTTGATTCAATGGTGGATACTGAGTTTGGGTATGTAGAAGGTGGTAGCAGGGCAGAAGGACGAAACAGAACTCCAGGTCAGAGCAAGAGATGGTGGCTCCCATCACCTCAGGTACCAGCTACTGGACTCTCTGATACAGCAAAGAATAAGTTACTTAAACAGAGTAAGGTGGTACATCAAGTATTCAAGGCTGCCAAAACCATCAACGAAAGTGTTCTTCTTGAAATGCATGTGCCAACCATTATCAGGGATGCACTTCCCAAGGTAGTGAAATTTTTTTCTGGTTTAGTATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

238

Amino Acids

26.63

Weight (kDa)

9.37

Isoelectric Point (pI)

43.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRONE PF03759 23 - 231 8.4e-101 PRONE (Plant-specific Rop nucleotide exchanger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 48
Acc65I GGTACC 1 cut(s) 532
AccB1I GGYRCC 1 cut(s) 532
AccB7I CCANNNNNTGG 1 cut(s) 542
AccI GTMKAC 1 cut(s) 62
AccII CGCG 1 cut(s) 53
AcoI YGGCCR 1 cut(s) 325
AcsI RAATTY 1 cut(s) 692
AcuI CTGAAG 1 cut(s) 246
AfaI GTAC 3 cut(s) 131, 534, 594
AfiI CCNNNNNNNGG 2 cut(s) 542, 666
AflII CTTAAG 1 cut(s) 389
AflIII ACRYGT 2 cut(s) 16, 51
AgsI TTSAA 4 cut(s) 416, 429, 607, 644
AjnI CCWGG 2 cut(s) 305, 493
AluBI AGCT 5 cut(s) 100, 202, 295, 356, 539
AluI AGCT 5 cut(s) 100, 202, 295, 356, 539
AlwNI CAGNNNCTG 1 cut(s) 542
AoxI GGCC 2 cut(s) 136, 325
ApeKI GCWGC 3 cut(s) 100, 182, 611
ApoI RAATTY 1 cut(s) 692
Asp700I GAANNNNTTC 1 cut(s) 633
Asp718I GGTACC 1 cut(s) 532
AspS9I GGNCC 1 cut(s) 136
AsuHPI GGTGA 2 cut(s) 122, 516
AxyI CCTNAGG 1 cut(s) 528
BanI GGYRCC 1 cut(s) 532
BauI CACGAG 1 cut(s) 357
BbvCI CCTCAGC 1 cut(s) 140
BbvI GCAGC 3 cut(s) 87, 194, 598
BccI CCATC 4 cut(s) 176, 504, 529, 629
BciT130I CCWGG 2 cut(s) 307, 495
BciVI GTATCC 1 cut(s) 430
BfaI CTAG 2 cut(s) 179, 296
BfrI CTTAAG 1 cut(s) 389
BfuI GTATCC 1 cut(s) 430
BisI GCNGC 3 cut(s) 101, 183, 612
BlsI GCNGC 3 cut(s) 102, 184, 613
Bme1390I CCNGG 2 cut(s) 307, 495
BmgT120I GGNCC 1 cut(s) 136
BmiI GGNNCC 3 cut(s) 216, 518, 534
BmrFI CCNGG 2 cut(s) 307, 495
BmsI GCATC 3 cut(s) 24, 396, 661
BpmI CTGGAG 1 cut(s) 477
Bpu10I CCTNAGC 1 cut(s) 140
BsaJI CCNNGG 1 cut(s) 681
Bsc4I CCNNNNNNNGG 2 cut(s) 542, 666
Bse1I ACTGG 3 cut(s) 216, 266, 547
Bse21I CCTNAGG 1 cut(s) 528
Bse3DI GCAATG 1 cut(s) 84
BseBI CCWGG 2 cut(s) 307, 495
BseDI CCNNGG 1 cut(s) 681
BseGI GGATG 1 cut(s) 676
BseLI CCNNNNNNNGG 2 cut(s) 542, 666
BseMI GCAATG 1 cut(s) 84
BseMII CTCAG 3 cut(s) 154, 432, 542
BseNI ACTGG 3 cut(s) 216, 266, 547
BseXI GCAGC 3 cut(s) 87, 194, 598
BsgI GTGCAG 1 cut(s) 381
Bsh1236I CGCG 1 cut(s) 53
BshFI GGCC 2 cut(s) 138, 327
BshNI GGYRCC 1 cut(s) 532
BsiSI CCGG 1 cut(s) 328
BslI CCNNNNNNNGG 2 cut(s) 542, 666
BsnI GGCC 2 cut(s) 138, 327
BspANI GGCC 2 cut(s) 138, 327
BspCNI CTCAG 3 cut(s) 153, 433, 541
BspFNI CGCG 1 cut(s) 53
BspLI GGNNCC 3 cut(s) 216, 518, 534
BspT107I GGYRCC 1 cut(s) 532
BspTI CTTAAG 1 cut(s) 389
BsrDI GCAATG 1 cut(s) 84
BsrI ACTGG 3 cut(s) 216, 266, 547
BssECI CCNNGG 1 cut(s) 681
BssNAI GTATAC 1 cut(s) 63
BssSI CACGAG 1 cut(s) 357
BssT1I CCWWGG 1 cut(s) 681
Bst1107I GTATAC 1 cut(s) 63
Bst2BI CACGAG 1 cut(s) 357
Bst2UI CCWGG 2 cut(s) 307, 495
Bst4CI ACNGT 2 cut(s) 190, 334
Bst6I CTCTTC 1 cut(s) 222
BstAFI CTTAAG 1 cut(s) 389
BstDEI CTNAG 3 cut(s) 140, 441, 528
BstF5I GGATG 1 cut(s) 676
BstFNI CGCG 1 cut(s) 53
BstNI CCWGG 2 cut(s) 307, 495
BstNSI RCATGY 2 cut(s) 20, 653
BstSCI CCNGG 2 cut(s) 305, 493
BstUI CGCG 1 cut(s) 53
BstV1I GCAGC 3 cut(s) 87, 194, 598
BstXI CCANNNNNNTGG 1 cut(s) 286
BstZ17I GTATAC 1 cut(s) 63
Bsu36I CCTNAGG 1 cut(s) 528
BsuI GTATCC 1 cut(s) 430
BsuRI GGCC 2 cut(s) 138, 327
BtsCI GGATG 1 cut(s) 676
BtsI GCAGTG 1 cut(s) 144
BtsIMutI CAGTG 1 cut(s) 144
CaiI CAGNNNCTG 1 cut(s) 542
Cfr13I GGNCC 1 cut(s) 136
Csp6I GTAC 3 cut(s) 130, 533, 593
CviAII CATG 2 cut(s) 17, 650
CviJI RGCY 9 cut(s) 100, 138, 202, 295, 327, 356, 517, 539, 611
CviKI_1 RGCY 9 cut(s) 100, 138, 202, 295, 327, 356, 517, 539, 611
CviQI GTAC 3 cut(s) 130, 533, 593
DdeI CTNAG 3 cut(s) 140, 441, 528
EaeI YGGCCR 1 cut(s) 325
Eam1104I CTCTTC 1 cut(s) 222
EarI CTCTTC 1 cut(s) 222
Eco130I CCWWGG 1 cut(s) 681
Eco57I CTGAAG 1 cut(s) 246
Eco81I CCTNAGG 1 cut(s) 528
EcoO109I RGGNCCY 1 cut(s) 136
EcoRII CCWGG 2 cut(s) 305, 493
EcoT14I CCWWGG 1 cut(s) 681
EcoT22I ATGCAT 1 cut(s) 651
ErhI CCWWGG 1 cut(s) 681
FaeI CATG 2 cut(s) 20, 653
FaiI YATR 9 cut(s) 18, 48, 63, 285, 287, 344, 368, 453, 651
FatI CATG 2 cut(s) 16, 649
FblI GTMKAC 1 cut(s) 62
Fnu4HI GCNGC 3 cut(s) 101, 183, 612
FokI GGATG 1 cut(s) 683
Fsp4HI GCNGC 3 cut(s) 101, 183, 612
FspBI CTAG 2 cut(s) 179, 296
GluI GCNGC 3 cut(s) 101, 183, 612
GsuI CTGGAG 1 cut(s) 477
HaeIII GGCC 2 cut(s) 138, 327
HapII CCGG 1 cut(s) 328
Hin1II CATG 2 cut(s) 20, 653
HinfI GANTC 4 cut(s) 346, 401, 425, 546
HpaII CCGG 1 cut(s) 328
HphI GGTGA 2 cut(s) 122, 516
Hpy166II GTNNAC 2 cut(s) 63, 93
Hpy188I TCNGA 4 cut(s) 226, 351, 501, 553
Hpy188III TCNNGA 2 cut(s) 641, 714
Hpy8I GTNNAC 2 cut(s) 63, 93
HpyAV CCTTC 3 cut(s) 312, 452, 470
HpyCH4III ACNGT 2 cut(s) 190, 334
HpyCH4V TGCA 3 cut(s) 362, 649, 674
HpyF3I CTNAG 3 cut(s) 140, 441, 528
Hsp92II CATG 2 cut(s) 20, 653
KpnI GGTACC 1 cut(s) 536
LmnI GCTCC 1 cut(s) 522
Lsp1109I GCAGC 3 cut(s) 87, 194, 598
LweI GCATC 3 cut(s) 24, 396, 661
MaeI CTAG 2 cut(s) 179, 296
MaeIII GTNAC 2 cut(s) 264, 570
MboII GAAGA 5 cut(s) 14, 125, 239, 371, 629
MluCI AATT 1 cut(s) 692
MluI ACGCGT 1 cut(s) 51
MlyI GAGTC 3 cut(s) 340, 395, 540
MmeI TCCRAC 1 cut(s) 378
MnlI CCTC 5 cut(s) 67, 119, 149, 225, 537
Mph1103I ATGCAT 1 cut(s) 651
MroXI GAANNNNTTC 1 cut(s) 633
MseI TTAA 2 cut(s) 390, 576
MspCI CTTAAG 1 cut(s) 389
MspI CCGG 1 cut(s) 328
MspR9I CCNGG 2 cut(s) 307, 495
MvaI CCWGG 2 cut(s) 307, 495
MvnI CGCG 1 cut(s) 53
NlaIII CATG 2 cut(s) 20, 653
NlaIV GGNNCC 3 cut(s) 216, 518, 534
NsiI ATGCAT 1 cut(s) 651
NspI RCATGY 2 cut(s) 20, 653
PciI ACATGT 1 cut(s) 16
PdmI GAANNNNTTC 1 cut(s) 633
PfeI GAWTC 1 cut(s) 425
PflMI CCANNNNNTGG 1 cut(s) 542
PkrI GCNGC 3 cut(s) 102, 184, 613
PleI GAGTC 3 cut(s) 340, 395, 540
PpsI GAGTC 3 cut(s) 340, 395, 540
PscI ACATGT 1 cut(s) 16
PsiI TTATAA 1 cut(s) 48
Psp6I CCWGG 2 cut(s) 305, 493
PspGI CCWGG 2 cut(s) 305, 493
PspN4I GGNNCC 3 cut(s) 216, 518, 534
PspPI GGNCC 1 cut(s) 136
PstNI CAGNNNCTG 1 cut(s) 542
RsaI GTAC 3 cut(s) 131, 534, 594
RsaNI GTAC 3 cut(s) 130, 533, 593
SaqAI TTAA 2 cut(s) 390, 576
SatI GCNGC 3 cut(s) 101, 183, 612
Sau96I GGNCC 1 cut(s) 136
SchI GAGTC 3 cut(s) 340, 395, 540
ScrFI CCNGG 2 cut(s) 307, 495
SfaNI GCATC 3 cut(s) 24, 396, 661
SmlI CTYRAG 1 cut(s) 389
SmoI CTYRAG 1 cut(s) 389
Sse9I AATT 1 cut(s) 692
SspMI CTAG 2 cut(s) 179, 296
StyD4I CCNGG 2 cut(s) 305, 493
StyI CCWWGG 1 cut(s) 681
TaaI ACNGT 2 cut(s) 190, 334
TaqI TCGA 1 cut(s) 404
TasI AATT 1 cut(s) 692
TfiI GAWTC 1 cut(s) 425
Tru1I TTAA 2 cut(s) 390, 576
Tru9I TTAA 2 cut(s) 390, 576
TscAI CASTG 1 cut(s) 151
TseI GCWGC 3 cut(s) 100, 182, 611
TspDTI ATGAA 1 cut(s) 95
TspRI CASTG 1 cut(s) 151
Van91I CCANNNNNTGG 1 cut(s) 542
Vha464I CTTAAG 1 cut(s) 389
XapI RAATTY 1 cut(s) 692
XceI RCATGY 2 cut(s) 20, 653
XmiI GTMKAC 1 cut(s) 62
XmnI GAANNNNTTC 1 cut(s) 633
XspI CTAG 2 cut(s) 179, 296
Zsp2I ATGCAT 1 cut(s) 651
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.