Rroxscaffold_4G00308310

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000821
Physical Location & Seq
Forward (+)
29840892 .. 29843510
2619 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00308310.1

Sequence Viewer

Length: 1497 bp
ATGTTCGGATCGACCGAAGGCAAAACTGTCATTTTTCTTGAAAAAGGGTCAAAGTCGACTTCACAGCTGGAGGGTCAGAAAGCTAAGAGTGAAGCTGTACAAAAGTCGGACAAGCCGGAAAAGTGGTGTATCTATAGGGTTCCCAGCAAGCTGCGCCGTGTCAATGAAGCAGCATATACCCCTCAATTACTTTCAATTGGCCTTTTCCACCATGGCAAAGCAGAATTGAGGGACATGGAGGACCATAAAAAAAAATATGTGAATTACTTTTTTGACCGCACTTTGAAAGATAAGGCTGCAGTTAGAAGATTCATTGAAGAACGTCGAAAGAAAATTCTTCATTGTTATGCAGGGAAGATTGAGCTCGATGAAGGCACTGCTGTTGATATAATTCTAAATGATGCATGCTTCATCATTGAGCTCTTTCTGATGAAGTCCGAAAGAGAAAATGGTGATGAATACAAAAAGGATTACATATTAAGTGCACCTTGGATGTGGAAAGCTGCAGAGCAGGATCTGATACTGTTTGAAAATCAGCTTCCTTATTTTCTTCTTCAAGAACTATATGACTTTGCATTTCCCGGAGCGTCTTCCATTTCCCATCCTGGAAAAGAAAAACAATCACATAGATCCAGCACCAATAACAAGCGGAACTGCTTGGTTTGCTTCCAATACTGCTTCCCTTGCTCCTGTGGGATCCATCCCAACGATCGTCACGTTATAAAGATGGATCATAAGTCTGAGCCTGATCATCACGTTATAAAGATGGATCATGAGTCCGAGCCTGAACGTATTCATGATCGCTTTCTTGAGCTGGCCTGTAATTTTTTCAAAAATTACAGTAACGGAAAATCTGTTGGGGTAAAACCAAAACATTTCACTGATTTGGTAAGACATTTTATGTGTCCAGAAGAAGCAACGAAGTGGAAAGAAAATACTACCAATAGTCTATACAATATGAGAAAGCTGACGGCAGCAGGGGTTAACTTTATGCCATTTCCTAAAGATGGACGCTTTGTGGTGAAAGAAGGTAACATTTTAAAGTTAACATGTTTTAAAATGATGGACTTGAAGCTTTCACAGTTTTGGGTAAAGGATGAGACGGAATGTATTATTCGCAATGTCATGGCAGTTGAGCAGTTTCTGTATCCAGACCATGCTTATGTTTGCAATTACTTCTTGCTGATGGATAAACTTGTGGACACTGTGGAAGATGTGGATTTGCTGATTCGGAATAAAGTGATCATTAACATGCTAGGCAGCAACGAAGCAGTGGCGGAACTGGTGAACCACCTATGTGATCAGATTCTCGAAGAAAGATCCTGCTATGTTGATATTTGTAAAAAACTAAATGACCACTACGAGAATTCTTGGAACCGTTGCGTGACAACCCTGAAACGAGTTTACTTCAAGGATCTGTGGACAGGCAGTTCAACTTTTTTCGGGGTTGTTGTACTGATTTTCTCTATCATTGGATCCATCAAGTCATTCAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

498

Amino Acids

58.06

Weight (kDa)

8.17

Isoelectric Point (pI)

40.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 44 - 488 2e-92 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 722, 761
AccI GTMKAC 1 cut(s) 56
AciI CCGC 3 cut(s) 277, 649, 1277
AcsI RAATTY 2 cut(s) 333, 1366
AfaI GTAC 2 cut(s) 99, 1455
AfiI CCNNNNNNNGG 1 cut(s) 1007
AflIII ACRYGT 1 cut(s) 1049
AjnI CCWGG 1 cut(s) 604
AleI CACNNNNGTG 1 cut(s) 1296
AloI GAACNNNNNNTCC 2 cut(s) 1414, 1446
Alw21I GWGCWC 3 cut(s) 366, 423, 487
Alw26I GTCTC 1 cut(s) 1094
Alw44I GTGCAC 1 cut(s) 483
AlwNI CAGNNNCTG 2 cut(s) 517, 1144
AoxI GGCC 2 cut(s) 199, 816
ApaLI GTGCAC 1 cut(s) 483
ApeKI GCWGC 6 cut(s) 151, 170, 296, 503, 974, 1260
ApoI RAATTY 2 cut(s) 333, 1366
Asp700I GAANNNNTTC 1 cut(s) 792
AspLEI GCGC 1 cut(s) 156
AspS9I GGNCC 1 cut(s) 241
AsuC2I CCSGG 1 cut(s) 582
AsuHPI GGTGA 3 cut(s) 464, 1033, 1297
AvaII GGWCC 1 cut(s) 241
BaeGI GKGCMC 1 cut(s) 487
BamHI GGATCC 2 cut(s) 696, 1475
BanII GRGCYC 2 cut(s) 366, 423
BbsI GAAGAC 1 cut(s) 582
Bbv12I GWGCWC 3 cut(s) 366, 423, 487
BbvI GCAGC 6 cut(s) 138, 182, 283, 490, 986, 1272
BccI CCATC 8 cut(s) 609, 708, 721, 760, 1001, 1057, 1180, 1487
BceAI ACGGC 2 cut(s) 141, 987
BciT130I CCWGG 1 cut(s) 606
BciVI GTATCC 1 cut(s) 1158
BclI TGATCA 3 cut(s) 748, 1242, 1300
BcnI CCSGG 1 cut(s) 582
BcoDI GTCTC 1 cut(s) 1094
BfaI CTAG 1 cut(s) 1256
BfmI CTRYAG 3 cut(s) 133, 297, 504
BfuI GTATCC 1 cut(s) 1158
BisI GCNGC 6 cut(s) 152, 171, 297, 504, 975, 1261
BlsI GCNGC 6 cut(s) 153, 172, 298, 505, 976, 1262
Bme1390I CCNGG 2 cut(s) 582, 606
Bme18I GGWCC 1 cut(s) 241
BmgT120I GGNCC 1 cut(s) 241
BmiI GGNNCC 4 cut(s) 141, 698, 1376, 1477
BmrFI CCNGG 2 cut(s) 582, 606
BmsI GCATC 1 cut(s) 391
BpiI GAAGAC 1 cut(s) 582
BpmI CTGGAG 1 cut(s) 89
BpuEI CTTGAG 1 cut(s) 830
BpuMI CCSGG 1 cut(s) 582
BsaJI CCNNGG 2 cut(s) 211, 488
Bsc4I CCNNNNNNNGG 1 cut(s) 1007
Bse1I ACTGG 1 cut(s) 1287
Bse3DI GCAATG 1 cut(s) 1126
BseBI CCWGG 1 cut(s) 606
BseDI CCNNGG 2 cut(s) 211, 488
BseGI GGATG 4 cut(s) 498, 601, 700, 1102
BseLI CCNNNNNNNGG 1 cut(s) 1007
BseMI GCAATG 1 cut(s) 1126
BseMII CTCAG 1 cut(s) 732
BseNI ACTGG 1 cut(s) 1287
BseSI GKGCMC 1 cut(s) 487
BseXI GCAGC 6 cut(s) 138, 182, 283, 490, 986, 1272
BseYI CCCAGC 1 cut(s) 143
Bsh1285I CGRYCG 2 cut(s) 15, 712
BshFI GGCC 2 cut(s) 201, 818
BsiEI CGRYCG 2 cut(s) 15, 712
BsiHKAI GWGCWC 3 cut(s) 366, 423, 487
BsiSI CCGG 2 cut(s) 116, 582
BslFI GGGAC 1 cut(s) 245
BslI CCNNNNNNNGG 1 cut(s) 1007
BsmAI GTCTC 1 cut(s) 1094
BsmBI CGTCTC 1 cut(s) 1094
BsmFI GGGAC 1 cut(s) 245
BsnI GGCC 2 cut(s) 201, 818
Bsp1286I GDGCHC 3 cut(s) 366, 423, 487
Bsp1407I TGTACA 1 cut(s) 97
Bsp19I CCATGG 1 cut(s) 211
BspACI CCGC 3 cut(s) 277, 649, 1277
BspANI GGCC 2 cut(s) 201, 818
BspCNI CTCAG 1 cut(s) 733
BspHI TCATGA 2 cut(s) 772, 796
BspLI GGNNCC 4 cut(s) 141, 698, 1376, 1477
BspMAI CTGCAG 2 cut(s) 301, 508
BsrDI GCAATG 1 cut(s) 1126
BsrGI TGTACA 1 cut(s) 97
BsrI ACTGG 1 cut(s) 1287
BssECI CCNNGG 2 cut(s) 211, 488
BssT1I CCWWGG 2 cut(s) 211, 488
Bst2UI CCWGG 1 cut(s) 606
Bst4CI ACNGT 6 cut(s) 28, 525, 842, 1083, 1207, 1379
BstAUI TGTACA 1 cut(s) 97
BstC8I GCNNGC 3 cut(s) 149, 406, 816
BstDEI CTNAG 2 cut(s) 84, 741
BstDSI CCRYGG 1 cut(s) 211
BstF5I GGATG 4 cut(s) 498, 601, 700, 1102
BstHHI GCGC 1 cut(s) 156
BstMAI GTCTC 1 cut(s) 1094
BstMCI CGRYCG 2 cut(s) 15, 712
BstMWI GCNNNNNNNGC 3 cut(s) 153, 663, 684
BstNI CCWGG 1 cut(s) 606
BstNSI RCATGY 3 cut(s) 408, 1053, 1255
BstSCI CCNGG 2 cut(s) 580, 604
BstSFI CTRYAG 3 cut(s) 133, 297, 504
BstSLI GKGCMC 1 cut(s) 487
BstV1I GCAGC 6 cut(s) 138, 182, 283, 490, 986, 1272
BstV2I GAAGAC 1 cut(s) 582
BstX2I RGATCY 6 cut(s) 514, 629, 696, 1319, 1414, 1475
BstYI RGATCY 6 cut(s) 514, 629, 696, 1319, 1414, 1475
BsuI GTATCC 1 cut(s) 1158
BsuRI GGCC 2 cut(s) 201, 818
BtgI CCRYGG 1 cut(s) 211
BtsCI GGATG 4 cut(s) 498, 601, 700, 1102
BtsI GCAGTG 2 cut(s) 375, 1278
BtsIMutI CAGTG 4 cut(s) 375, 879, 1203, 1278
Cac8I GCNNGC 3 cut(s) 149, 406, 816
CaiI CAGNNNCTG 2 cut(s) 517, 1144
CciI TCATGA 2 cut(s) 772, 796
CfoI GCGC 1 cut(s) 156
Cfr13I GGNCC 1 cut(s) 241
CseI GACGC 2 cut(s) 576, 1020
Csp6I GTAC 2 cut(s) 98, 1454
CviAII CATG 9 cut(s) 212, 235, 405, 773, 797, 1050, 1126, 1157, 1252
CviQI GTAC 2 cut(s) 98, 1454
DdeI CTNAG 2 cut(s) 84, 741
DraI TTTAAA 2 cut(s) 1041, 1057
EciI GGCGGA 2 cut(s) 320, 1292
Ecl136II GAGCTC 2 cut(s) 364, 421
Eco130I CCWWGG 2 cut(s) 211, 488
Eco24I GRGCYC 2 cut(s) 366, 423
Eco47I GGWCC 1 cut(s) 241
Eco53kI GAGCTC 2 cut(s) 364, 421
EcoICRI GAGCTC 2 cut(s) 364, 421
EcoRI GAATTC 1 cut(s) 1366
EcoRII CCWGG 1 cut(s) 604
EcoT14I CCWWGG 2 cut(s) 211, 488
EcoT22I ATGCAT 1 cut(s) 406
EcoT38I GRGCYC 2 cut(s) 366, 423
ErhI CCWWGG 2 cut(s) 211, 488
Esp3I CGTCTC 1 cut(s) 1094
FaeI CATG 9 cut(s) 215, 238, 408, 776, 800, 1053, 1129, 1160, 1255
FalI AAGNNNNNCTT 2 cut(s) 472, 504
FaqI GGGAC 1 cut(s) 245
FatI CATG 9 cut(s) 211, 234, 404, 772, 796, 1049, 1125, 1156, 1251
FbaI TGATCA 3 cut(s) 748, 1242, 1300
FblI GTMKAC 1 cut(s) 56
Fnu4HI GCNGC 6 cut(s) 152, 171, 297, 504, 975, 1261
FokI GGATG 4 cut(s) 505, 588, 687, 1109
FriOI GRGCYC 2 cut(s) 366, 423
Fsp4HI GCNGC 6 cut(s) 152, 171, 297, 504, 975, 1261
FspBI CTAG 1 cut(s) 1256
GlaI GCGC 1 cut(s) 155
GluI GCNGC 6 cut(s) 152, 171, 297, 504, 975, 1261
GsaI CCCAGC 1 cut(s) 147
GsuI CTGGAG 1 cut(s) 89
HaeIII GGCC 2 cut(s) 201, 818
HapII CCGG 2 cut(s) 116, 582
HgaI GACGC 2 cut(s) 576, 1020
HhaI GCGC 1 cut(s) 156
Hin1II CATG 9 cut(s) 215, 238, 408, 776, 800, 1053, 1129, 1160, 1255
Hin6I GCGC 1 cut(s) 154
HinP1I GCGC 1 cut(s) 154
HincII GTYRAC 3 cut(s) 57, 985, 1047
HindII GTYRAC 3 cut(s) 57, 985, 1047
HindIII AAGCTT 1 cut(s) 1073
HinfI GANTC 4 cut(s) 309, 776, 1228, 1306
HpaI GTTAAC 2 cut(s) 985, 1047
HpaII CCGG 2 cut(s) 116, 582
HphI GGTGA 3 cut(s) 464, 1033, 1297
Hpy166II GTNNAC 8 cut(s) 57, 485, 985, 1047, 1201, 1288, 1405, 1422
Hpy188III TCNNGA 8 cut(s) 38, 557, 773, 797, 809, 908, 1151, 1310
Hpy8I GTNNAC 8 cut(s) 57, 485, 985, 1047, 1201, 1288, 1405, 1422
Hpy99I CGWCG 1 cut(s) 327
HpyAV CCTTC 3 cut(s) 11, 365, 1022
HpyCH4III ACNGT 6 cut(s) 28, 525, 842, 1083, 1207, 1379
HpyCH4IV ACGT 4 cut(s) 322, 717, 756, 790
HpyCH4V TGCA 7 cut(s) 299, 350, 404, 485, 506, 575, 1170
HpyF10VI GCNNNNNNNGC 3 cut(s) 153, 663, 684
HpyF3I CTNAG 2 cut(s) 84, 741
HpySE526I ACGT 4 cut(s) 322, 717, 756, 790
Hsp92II CATG 9 cut(s) 215, 238, 408, 776, 800, 1053, 1129, 1160, 1255
HspAI GCGC 1 cut(s) 154
Ksp22I TGATCA 3 cut(s) 748, 1242, 1300
KspAI GTTAAC 2 cut(s) 985, 1047
LmnI GCTCC 2 cut(s) 584, 692
Lsp1109I GCAGC 6 cut(s) 138, 182, 283, 490, 986, 1272
LweI GCATC 1 cut(s) 391
MaeI CTAG 1 cut(s) 1256
MaeII ACGT 4 cut(s) 322, 717, 756, 790
MaeIII GTNAC 4 cut(s) 713, 842, 1031, 1384
MfeI CAATTG 1 cut(s) 195
MflI RGATCY 6 cut(s) 514, 629, 696, 1319, 1414, 1475
MhlI GDGCHC 3 cut(s) 366, 423, 487
MlyI GAGTC 1 cut(s) 785
MmeI TCCRAC 1 cut(s) 87
MnlI CCTC 4 cut(s) 64, 192, 222, 232
Mph1103I ATGCAT 1 cut(s) 406
MroXI GAANNNNTTC 1 cut(s) 792
MseI TTAA 6 cut(s) 479, 984, 1040, 1046, 1056, 1248
MslI CAYNNNNRTG 4 cut(s) 345, 1161, 1250, 1296
MspA1I CMGCKG 1 cut(s) 67
MspI CCGG 2 cut(s) 116, 582
MspR9I CCNGG 2 cut(s) 582, 606
MunI CAATTG 1 cut(s) 195
MvaI CCWGG 1 cut(s) 606
MwoI GCNNNNNNNGC 3 cut(s) 153, 663, 684
NciI CCSGG 1 cut(s) 582
NcoI CCATGG 1 cut(s) 211
NlaIII CATG 9 cut(s) 215, 238, 408, 776, 800, 1053, 1129, 1160, 1255
NlaIV GGNNCC 4 cut(s) 141, 698, 1376, 1477
NmuCI GTSAC 2 cut(s) 713, 1384
NsiI ATGCAT 1 cut(s) 406
NspI RCATGY 3 cut(s) 408, 1053, 1255
OliI CACNNNNGTG 1 cut(s) 1296
PaeI GCATGC 1 cut(s) 408
PagI TCATGA 2 cut(s) 772, 796
PciI ACATGT 1 cut(s) 1049
PcsI WCGNNNNNNNCGW 2 cut(s) 12, 714
PctI GAATGC 2 cut(s) 858, 958
PdmI GAANNNNTTC 1 cut(s) 792
PfeI GAWTC 3 cut(s) 309, 1228, 1306
PfoI TCCNGGA 2 cut(s) 580, 604
PkrI GCNGC 6 cut(s) 153, 172, 298, 505, 976, 1262
Ple19I CGATCG 1 cut(s) 712
PleI GAGTC 1 cut(s) 784
PpsI GAGTC 1 cut(s) 784
PscI ACATGT 1 cut(s) 1049
PsiI TTATAA 2 cut(s) 722, 761
Psp124BI GAGCTC 2 cut(s) 366, 423
Psp6I CCWGG 1 cut(s) 604
PspFI CCCAGC 1 cut(s) 143
PspGI CCWGG 1 cut(s) 604
PspN4I GGNNCC 4 cut(s) 141, 698, 1376, 1477
PspPI GGNCC 1 cut(s) 241
PstI CTGCAG 2 cut(s) 301, 508
PstNI CAGNNNCTG 2 cut(s) 517, 1144
PsuI RGATCY 6 cut(s) 514, 629, 696, 1319, 1414, 1475
PvuI CGATCG 1 cut(s) 712
PvuII CAGCTG 1 cut(s) 67
RsaI GTAC 2 cut(s) 99, 1455
RsaNI GTAC 2 cut(s) 98, 1454
RseI CAYNNNNRTG 4 cut(s) 345, 1161, 1250, 1296
SacI GAGCTC 2 cut(s) 366, 423
SalI GTCGAC 1 cut(s) 55
SaqAI TTAA 6 cut(s) 479, 984, 1040, 1046, 1056, 1248
SatI GCNGC 6 cut(s) 152, 171, 297, 504, 975, 1261
Sau96I GGNCC 1 cut(s) 241
SchI GAGTC 1 cut(s) 785
ScrFI CCNGG 2 cut(s) 582, 606
SduI GDGCHC 3 cut(s) 366, 423, 487
SfaNI GCATC 1 cut(s) 391
SfcI CTRYAG 3 cut(s) 133, 297, 504
SinI GGWCC 1 cut(s) 241
SmiMI CAYNNNNRTG 4 cut(s) 345, 1161, 1250, 1296
SmlI CTYRAG 1 cut(s) 809
SmoI CTYRAG 1 cut(s) 809
SphI GCATGC 1 cut(s) 408
SsiI CCGC 3 cut(s) 277, 649, 1277
SspMI CTAG 1 cut(s) 1256
SstI GAGCTC 2 cut(s) 366, 423
StyD4I CCNGG 2 cut(s) 580, 604
StyI CCWWGG 2 cut(s) 211, 488
TaaI ACNGT 6 cut(s) 28, 525, 842, 1083, 1207, 1379
TaiI ACGT 4 cut(s) 325, 720, 759, 793
TaqI TCGA 5 cut(s) 11, 56, 325, 366, 1311
TaqII GACCGA 1 cut(s) 29
TatI WGTACW 2 cut(s) 97, 1453
TfiI GAWTC 3 cut(s) 309, 1228, 1306
Tru1I TTAA 6 cut(s) 479, 984, 1040, 1046, 1056, 1248
Tru9I TTAA 6 cut(s) 479, 984, 1040, 1046, 1056, 1248
TscAI CASTG 4 cut(s) 382, 886, 1210, 1278
TseFI GTSAC 2 cut(s) 713, 1384
TseI GCWGC 6 cut(s) 151, 170, 296, 503, 974, 1260
Tsp45I GTSAC 2 cut(s) 713, 1384
TspDTI ATGAA 8 cut(s) 180, 301, 329, 384, 400, 446, 471, 785
TspGWI ACGGA 2 cut(s) 861, 1118
TspRI CASTG 4 cut(s) 382, 886, 1210, 1278
VneI GTGCAC 1 cut(s) 483
VpaK11BI GGWCC 1 cut(s) 241
XapI RAATTY 2 cut(s) 333, 1366
XceI RCATGY 3 cut(s) 408, 1053, 1255
XmiI GTMKAC 1 cut(s) 56
XmnI GAANNNNTTC 1 cut(s) 792
XspI CTAG 1 cut(s) 1256
Zsp2I ATGCAT 1 cut(s) 406
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.