FvH4_7g09700

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0000821
Physical Location & Seq
Forward (+)
9314041 .. 9315748
1708 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g09700.t1

Sequence Viewer

Length: 1383 bp
ATGGTGGAACCAACCGATTCTGAGAATGAACAAAGCAAGAATGCGCCCGTATTATTTTCGAACGAATGGTCCATCTACAGGGTTCCCAGCCAACTCCGCAATGTAGATGAAGCAGCGTACACTCCTCAAACACTTTCAATAGGACCTTTCCACCATGGAAATCCAAAATTGAGGGACATGGAGGCTCATAAGAAGATATGCTATGAGAGGTTTCGCAATCGTACTTTGAAGACTGAGGAGGAGCTAAGGATCTTCATGATGGATCAGCAAAAAAGAATTCTAGATTGTTATGCAGGTACCTTTAAATGTGACATCGACTTTTTGAACGTCATCCTAGTTGACGCTTGCTTCATTATTGAACTCTTTTTGAGTAATTCTGAACAAGAACATCATAATGCAAATTACTATATAATGAGATCACCGTGGCTGAGGAAATCTGTGGAGAGGGACCTAATACTGTTTGAAAATCAGCTTCCTTATTTTCTTCTTCAAGAGCTATATGACTTCGCCACACCTATCTTTTCCGATGGCCATTCTGGCAATGGATACCAATCACAAGGACATGCCCATAACGAAAGCACCAGAAGTCAGCAGTGCTGCTTCCGTTGCTTCCGTCCCGGTGATTTGCAAGAGAACTCCATCCAATCTGAACCTTTCCAGCATCGCTTTCTTGAGCTTGCCTGCAAGTTCTTTAAGGAATACAGTAAGGGAAAATCTGTCAGGAATAGATTTAAGCCGAAACACTTCACTGATTTGGTTAGACACTTTCTTTGTCCATACAAGGAACTGTCGTGGGTAGAAAATTATGATGACCCTGTCAAAATTAAATACAATGCAAGAAAGCTGAAGGCTGCAGGAGTGAAGTTTATGCCACTTAGAGAACCTTTTGTTATAAAGGAAGACCCTAAGTTGACGTTAATTAACTTAGCATGTTCAATGCATAAGGAAATGAAGCTTACAAGACTTTGGGCAGAGTATGAGGCAGTAGGCATTTTCCGAAACACCATGGCCTTGGAACAGCTTATATATCCAAACAATCCTTATATTTGCGGTTATTTTTTGCTGATGAATCAACTCATCAAAACTGAGGAAGACGTGGAAGTTCTGATTGAAGATGGAGTAATCTTCAACTTGCTAGGCAGCAACAAAGCAGTGTTAAACATGATTAATTCATTGTGTGAGGAGATTAAGGAGAACCCATCCTGCTATGCTGAGATCTGTCAACACATTAATAAGCATTATAAAATGGTTTTGTATCACCCTTTGGTAAGGATGAGACAGGTTTACTTCAAGGATCTTTGGAGAGCTAGTATAGCTATACTAGGATTCGTTGTACTGGTTTTGACAGTTCTCACATCCCTTCGGACATTCAAACTGATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

461

Amino Acids

54.16

Weight (kDa)

7.54

Isoelectric Point (pI)

49.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 25 - 448 2.8e-87 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 893, 1242
Acc36I ACCTGC 1 cut(s) 284
Acc65I GGTACC 1 cut(s) 296
AccB1I GGYRCC 1 cut(s) 296
AciI CCGC 2 cut(s) 97, 1050
AclWI GGATC 3 cut(s) 257, 270, 1302
AcoI YGGCCR 1 cut(s) 529
AcsI RAATTY 1 cut(s) 276
AcuI CTGAAG 1 cut(s) 866
AfaI GTAC 4 cut(s) 119, 223, 298, 1335
AfiI CCNNNNNNNGG 1 cut(s) 78
AjiI CACGTC 1 cut(s) 1096
AloI GAACNNNNNNTCC 2 cut(s) 53, 85
AluBI AGCT 9 cut(s) 244, 472, 496, 676, 844, 955, 1021, 1307, 1316
AluI AGCT 9 cut(s) 244, 472, 496, 676, 844, 955, 1021, 1307, 1316
Alw26I GTCTC 1 cut(s) 1270
AlwI GGATC 3 cut(s) 257, 270, 1302
AoxI GGCC 2 cut(s) 529, 1008
ApeKI GCWGC 4 cut(s) 113, 597, 851, 1140
ApoI RAATTY 1 cut(s) 276
ArsI GACNNNNNNTTYG 4 cut(s) 53, 85, 753, 785
AseI ATTAAT 2 cut(s) 1167, 1230
Asp700I GAANNNNTTC 1 cut(s) 743
Asp718I GGTACC 1 cut(s) 296
AspLEI GCGC 1 cut(s) 46
AspS9I GGNCC 3 cut(s) 69, 143, 448
AsuC2I CCSGG 1 cut(s) 618
AsuHPI GGTGA 3 cut(s) 411, 632, 1250
AsuII TTCGAA 1 cut(s) 59
AvaII GGWCC 3 cut(s) 69, 143, 448
BalI TGGCCA 1 cut(s) 531
BanI GGYRCC 1 cut(s) 296
BbsI GAAGAC 3 cut(s) 236, 906, 1098
BbvCI CCTCAGC 1 cut(s) 428
BbvI GCAGC 4 cut(s) 125, 584, 838, 1152
BccI CCATC 6 cut(s) 80, 253, 521, 647, 1109, 1207
BciVI GTATCC 1 cut(s) 539
BcnI CCSGG 1 cut(s) 618
BcoDI GTCTC 1 cut(s) 1270
BfaI CTAG 5 cut(s) 281, 335, 1136, 1308, 1322
BfmI CTRYAG 2 cut(s) 76, 852
BfuAI ACCTGC 1 cut(s) 284
BfuI GTATCC 1 cut(s) 539
BglI GCCNNNNNGGC 1 cut(s) 537
BglII AGATCT 1 cut(s) 1215
BisI GCNGC 4 cut(s) 114, 598, 852, 1141
BlsI GCNGC 4 cut(s) 115, 599, 853, 1142
Bme1390I CCNGG 1 cut(s) 618
Bme18I GGWCC 3 cut(s) 69, 143, 448
BmgBI CACGTC 1 cut(s) 1096
BmgT120I GGNCC 3 cut(s) 69, 143, 448
BmiI GGNNCC 4 cut(s) 9, 84, 298, 449
BmrFI CCNGG 1 cut(s) 618
BmsI GCATC 1 cut(s) 670
BpiI GAAGAC 3 cut(s) 236, 906, 1098
Bpu10I CCTNAGC 2 cut(s) 245, 428
Bpu14I TTCGAA 1 cut(s) 59
BpuEI CTTGAG 1 cut(s) 692
BpuMI CCSGG 1 cut(s) 618
BsaBI GATNNNNATC 1 cut(s) 550
BsaJI CCNNGG 4 cut(s) 154, 422, 1005, 1011
BsaXI ACNNNNNCTCC 2 cut(s) 1294, 1324
Bsc4I CCNNNNNNNGG 1 cut(s) 78
Bse1I ACTGG 1 cut(s) 1341
Bse3DI GCAATG 2 cut(s) 106, 547
Bse8I GATNNNNATC 1 cut(s) 550
BseDI CCNNGG 4 cut(s) 154, 422, 1005, 1011
BseGI GGATG 5 cut(s) 330, 639, 1199, 1278, 1355
BseJI GATNNNNATC 1 cut(s) 550
BseLI CCNNNNNNNGG 1 cut(s) 78
BseMI GCAATG 2 cut(s) 106, 547
BseMII CTCAG 5 cut(s) 12, 225, 419, 1077, 1203
BseNI ACTGG 1 cut(s) 1341
BseRI GAGGAG 4 cut(s) 114, 251, 254, 1196
BseXI GCAGC 4 cut(s) 125, 584, 838, 1152
BseYI CCCAGC 1 cut(s) 86
BshFI GGCC 2 cut(s) 531, 1010
BshNI GGYRCC 1 cut(s) 296
BsiSI CCGG 1 cut(s) 618
BslFI GGGAC 3 cut(s) 188, 461, 600
BslI CCNNNNNNNGG 1 cut(s) 78
BsmAI GTCTC 1 cut(s) 1270
BsmFI GGGAC 3 cut(s) 188, 461, 600
BsmI GAATGC 1 cut(s) 46
BsnI GGCC 2 cut(s) 531, 1010
Bsp119I TTCGAA 1 cut(s) 59
Bsp143I GATC 5 cut(s) 249, 262, 416, 1215, 1294
Bsp19I CCATGG 2 cut(s) 154, 1005
BspACI CCGC 2 cut(s) 97, 1050
BspANI GGCC 2 cut(s) 531, 1010
BspCNI CTCAG 5 cut(s) 13, 226, 420, 1078, 1204
BspHI TCATGA 1 cut(s) 255
BspLI GGNNCC 4 cut(s) 9, 84, 298, 449
BspMAI CTGCAG 1 cut(s) 856
BspMI ACCTGC 1 cut(s) 284
BspPI GGATC 3 cut(s) 257, 270, 1302
BspT104I TTCGAA 1 cut(s) 59
BspT107I GGYRCC 1 cut(s) 296
BsrDI GCAATG 2 cut(s) 106, 547
BsrI ACTGG 1 cut(s) 1341
BssECI CCNNGG 4 cut(s) 154, 422, 1005, 1011
BssMI GATC 5 cut(s) 249, 262, 416, 1215, 1294
BssT1I CCWWGG 3 cut(s) 154, 1005, 1011
Bst4CI ACNGT 5 cut(s) 423, 459, 704, 789, 1348
BstBI TTCGAA 1 cut(s) 59
BstC8I GCNNGC 3 cut(s) 346, 678, 682
BstDEI CTNAG 9 cut(s) 21, 234, 245, 428, 875, 906, 925, 1086, 1212
BstDSI CCRYGG 3 cut(s) 154, 422, 1005
BstF5I GGATG 5 cut(s) 330, 639, 1199, 1278, 1355
BstHHI GCGC 1 cut(s) 46
BstKTI GATC 5 cut(s) 252, 265, 419, 1218, 1297
BstMAI GTCTC 1 cut(s) 1270
BstMBI GATC 5 cut(s) 249, 262, 416, 1215, 1294
BstMWI GCNNNNNNNGC 4 cut(s) 96, 537, 606, 1313
BstNSI RCATGY 2 cut(s) 566, 933
BstSCI CCNGG 1 cut(s) 616
BstSFI CTRYAG 2 cut(s) 76, 852
BstV1I GCAGC 4 cut(s) 125, 584, 838, 1152
BstV2I GAAGAC 3 cut(s) 236, 906, 1098
BstX2I RGATCY 3 cut(s) 249, 1215, 1294
BstXI CCANNNNNNTGG 1 cut(s) 1012
BstYI RGATCY 3 cut(s) 249, 1215, 1294
BsuI GTATCC 1 cut(s) 539
BsuRI GGCC 2 cut(s) 531, 1010
BtgI CCRYGG 3 cut(s) 154, 422, 1005
BtgZI GCGATG 1 cut(s) 647
BtrI CACGTC 1 cut(s) 1096
BtsCI GGATG 5 cut(s) 330, 639, 1199, 1278, 1355
BtsI GCAGTG 2 cut(s) 599, 1158
BtsIMutI CAGTG 3 cut(s) 599, 747, 1158
BveI ACCTGC 1 cut(s) 284
Cac8I GCNNGC 3 cut(s) 346, 678, 682
CciI TCATGA 1 cut(s) 255
CfoI GCGC 1 cut(s) 46
Cfr13I GGNCC 3 cut(s) 69, 143, 448
CseI GACGC 1 cut(s) 350
Csp6I GTAC 4 cut(s) 118, 222, 297, 1334
CviAII CATG 7 cut(s) 155, 178, 256, 563, 930, 1006, 1162
CviQI GTAC 4 cut(s) 118, 222, 297, 1334
DdeI CTNAG 9 cut(s) 21, 234, 245, 428, 875, 906, 925, 1086, 1212
DpnI GATC 5 cut(s) 251, 264, 418, 1217, 1296
DpnII GATC 5 cut(s) 249, 262, 416, 1215, 1294
DraI TTTAAA 1 cut(s) 304
EaeI YGGCCR 1 cut(s) 529
Eco130I CCWWGG 3 cut(s) 154, 1005, 1011
Eco47I GGWCC 3 cut(s) 69, 143, 448
Eco57I CTGAAG 1 cut(s) 866
EcoO109I RGGNCCY 2 cut(s) 143, 448
EcoRI GAATTC 1 cut(s) 276
EcoT14I CCWWGG 3 cut(s) 154, 1005, 1011
EcoT22I ATGCAT 1 cut(s) 942
ErhI CCWWGG 3 cut(s) 154, 1005, 1011
FaeI CATG 7 cut(s) 158, 181, 259, 566, 933, 1009, 1165
FaqI GGGAC 3 cut(s) 188, 461, 600
FatI CATG 7 cut(s) 154, 177, 255, 562, 929, 1005, 1161
Fnu4HI GCNGC 4 cut(s) 114, 598, 852, 1141
FokI GGATG 5 cut(s) 317, 626, 1186, 1285, 1342
Fsp4HI GCNGC 4 cut(s) 114, 598, 852, 1141
FspBI CTAG 5 cut(s) 281, 335, 1136, 1308, 1322
GlaI GCGC 1 cut(s) 45
GluI GCNGC 4 cut(s) 114, 598, 852, 1141
GsaI CCCAGC 1 cut(s) 90
HaeIII GGCC 2 cut(s) 531, 1010
HapII CCGG 1 cut(s) 618
HgaI GACGC 1 cut(s) 350
HhaI GCGC 1 cut(s) 46
Hin1II CATG 7 cut(s) 158, 181, 259, 566, 933, 1009, 1165
Hin6I GCGC 1 cut(s) 44
HinP1I GCGC 1 cut(s) 44
HincII GTYRAC 3 cut(s) 340, 912, 1223
HindII GTYRAC 3 cut(s) 340, 912, 1223
HindIII AAGCTT 1 cut(s) 953
HinfI GANTC 3 cut(s) 17, 1069, 1326
HpaII CCGG 1 cut(s) 618
HphI GGTGA 3 cut(s) 411, 632, 1250
Hpy166II GTNNAC 5 cut(s) 120, 340, 912, 1223, 1285
Hpy188I TCNGA 7 cut(s) 22, 379, 526, 649, 998, 1107, 1365
Hpy188III TCNNGA 5 cut(s) 256, 281, 491, 671, 721
Hpy8I GTNNAC 5 cut(s) 120, 340, 912, 1223, 1285
HpyAV CCTTC 2 cut(s) 841, 1370
HpyCH4III ACNGT 5 cut(s) 423, 459, 704, 789, 1348
HpyCH4IV ACGT 3 cut(s) 327, 914, 1095
HpyCH4V TGCA 7 cut(s) 293, 398, 628, 684, 836, 854, 940
HpyF10VI GCNNNNNNNGC 4 cut(s) 96, 537, 606, 1313
HpyF3I CTNAG 9 cut(s) 21, 234, 245, 428, 875, 906, 925, 1086, 1212
HpySE526I ACGT 3 cut(s) 327, 914, 1095
Hsp92II CATG 7 cut(s) 158, 181, 259, 566, 933, 1009, 1165
HspAI GCGC 1 cut(s) 44
KpnI GGTACC 1 cut(s) 300
Kzo9I GATC 5 cut(s) 249, 262, 416, 1215, 1294
LmnI GCTCC 1 cut(s) 241
Lsp1109I GCAGC 4 cut(s) 125, 584, 838, 1152
LweI GCATC 1 cut(s) 670
MaeI CTAG 5 cut(s) 281, 335, 1136, 1308, 1322
MaeII ACGT 3 cut(s) 327, 914, 1095
MaeIII GTNAC 1 cut(s) 308
MalI GATC 5 cut(s) 251, 264, 418, 1217, 1296
MboI GATC 5 cut(s) 249, 262, 416, 1215, 1294
MboII GAAGA 9 cut(s) 205, 241, 244, 476, 479, 911, 1103, 1117, 1124
MflI RGATCY 3 cut(s) 249, 1215, 1294
MlsI TGGCCA 1 cut(s) 531
MluCI AATT 8 cut(s) 167, 276, 373, 400, 802, 822, 918, 1168
MluNI TGGCCA 1 cut(s) 531
Mox20I TGGCCA 1 cut(s) 531
Mph1103I ATGCAT 1 cut(s) 942
MroXI GAANNNNTTC 1 cut(s) 743
MscI TGGCCA 1 cut(s) 531
MslI CAYNNNNRTG 1 cut(s) 393
Msp20I TGGCCA 1 cut(s) 531
MspI CCGG 1 cut(s) 618
MspR9I CCNGG 1 cut(s) 618
Mva1269I GAATGC 1 cut(s) 46
MwoI GCNNNNNNNGC 4 cut(s) 96, 537, 606, 1313
NciI CCSGG 1 cut(s) 618
NcoI CCATGG 2 cut(s) 154, 1005
NdeII GATC 5 cut(s) 249, 262, 416, 1215, 1294
NlaIII CATG 7 cut(s) 158, 181, 259, 566, 933, 1009, 1165
NlaIV GGNNCC 4 cut(s) 9, 84, 298, 449
NmuCI GTSAC 1 cut(s) 308
NsiI ATGCAT 1 cut(s) 942
NspI RCATGY 2 cut(s) 566, 933
NspV TTCGAA 1 cut(s) 59
PacI TTAATTAA 1 cut(s) 921
PagI TCATGA 1 cut(s) 255
PctI GAATGC 1 cut(s) 46
PdmI GAANNNNTTC 1 cut(s) 743
PfeI GAWTC 3 cut(s) 17, 1069, 1326
PflFI GACNNNGTC 1 cut(s) 815
PkrI GCNGC 4 cut(s) 115, 599, 853, 1142
PpuMI RGGWCCY 2 cut(s) 143, 448
PshBI ATTAAT 2 cut(s) 1167, 1230
PsiI TTATAA 2 cut(s) 893, 1242
Psp5II RGGWCCY 2 cut(s) 143, 448
PspFI CCCAGC 1 cut(s) 86
PspN4I GGNNCC 4 cut(s) 9, 84, 298, 449
PspPI GGNCC 3 cut(s) 69, 143, 448
PspPPI RGGWCCY 2 cut(s) 143, 448
PstI CTGCAG 1 cut(s) 856
PsuI RGATCY 3 cut(s) 249, 1215, 1294
PsyI GACNNNGTC 1 cut(s) 815
RsaI GTAC 4 cut(s) 119, 223, 298, 1335
RsaNI GTAC 4 cut(s) 118, 222, 297, 1334
RseI CAYNNNNRTG 1 cut(s) 393
SatI GCNGC 4 cut(s) 114, 598, 852, 1141
Sau3AI GATC 5 cut(s) 249, 262, 416, 1215, 1294
Sau96I GGNCC 3 cut(s) 69, 143, 448
ScrFI CCNGG 1 cut(s) 618
SfaNI GCATC 1 cut(s) 670
SfcI CTRYAG 2 cut(s) 76, 852
SfuI TTCGAA 1 cut(s) 59
SinI GGWCC 3 cut(s) 69, 143, 448
SmiMI CAYNNNNRTG 1 cut(s) 393
SmlI CTYRAG 1 cut(s) 671
SmoI CTYRAG 1 cut(s) 671
Sse9I AATT 8 cut(s) 167, 276, 373, 400, 802, 822, 918, 1168
SsiI CCGC 2 cut(s) 97, 1050
SspMI CTAG 5 cut(s) 281, 335, 1136, 1308, 1322
StyD4I CCNGG 1 cut(s) 616
StyI CCWWGG 3 cut(s) 154, 1005, 1011
TaaI ACNGT 5 cut(s) 423, 459, 704, 789, 1348
TaiI ACGT 3 cut(s) 330, 917, 1098
TaqI TCGA 2 cut(s) 59, 315
TasI AATT 8 cut(s) 167, 276, 373, 400, 802, 822, 918, 1168
TatI WGTACW 1 cut(s) 1333
TfiI GAWTC 3 cut(s) 17, 1069, 1326
TscAI CASTG 3 cut(s) 599, 754, 1158
TseFI GTSAC 1 cut(s) 308
TseI GCWGC 4 cut(s) 113, 597, 851, 1140
Tsp45I GTSAC 1 cut(s) 308
TspDTI ATGAA 7 cut(s) 42, 123, 244, 340, 965, 1082, 1161
TspGWI ACGGA 2 cut(s) 593, 602
TspRI CASTG 3 cut(s) 599, 754, 1158
Tth111I GACNNNGTC 1 cut(s) 815
VpaK11BI GGWCC 3 cut(s) 69, 143, 448
VspI ATTAAT 2 cut(s) 1167, 1230
XapI RAATTY 1 cut(s) 276
XbaI TCTAGA 1 cut(s) 280
XceI RCATGY 2 cut(s) 566, 933
XcmI CCANNNNNNNNNTGG 1 cut(s) 539
XmnI GAANNNNTTC 1 cut(s) 743
XspI CTAG 5 cut(s) 281, 335, 1136, 1308, 1322
Zsp2I ATGCAT 1 cut(s) 942
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.