RchiOBHm_Chr1g0346611

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000821
Physical Location & Seq
Forward (+)
39063912 .. 39065237
1326 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ57287

Sequence Viewer

Length: 1326 bp
ATGGATGAACCGGACAAGTGGTGTATCTACAGGGTTCCTAGCAAACTCCGCAATGTAAATGAAGCAGCATACACTCCTCAGTTACTTTCCATCGGACTCTTCCACCATGGAAAACCAGAACTGGAGGACATGGAAACCCATAAGAAAATGTATACCGAGAATTTCTACTTGCGTTCTTGGAAAAGTAAGGATGATCTAAAAAGGTTCATCAGGGACCGTAAGGAAAAAATTCTGCGGTGTTATGCAGGGACTATCAAGATTCCCATCGAAAAGGATCCTGTGGAGATAATTTTAGTTGATGCCTGCTTCATCATTGAGCTCCTTTTGCTGAACTCCATAACAAAGAATCATGAAAATGATTACATATTAAGATCACCATGGCTGAGGAAAGCTGTTGAGCAGGAACTGATACTTTTCGAAAATCAGCTTCCTTATTCTCTTCTTGAGGACCTATATGAATTTGCTCAGCCCGTCCGTTTACATTACCAATCTCGCAAAGGACCACAAGCACAAGAACAGCCCCAGGAATACAGCACAGGTGATCAAGACAGTCCCCGTGATTCTTTAGAGATTGTCATCCATGATGCTAAACCAGTTCATAATAATTTTTTGGAGCTTGCCAGCAAGTTCTTCAAAGATCATAATGAGGGAAAGGTCATCACTAGTGGCGTAAAACCGAAACACTTCACGGATCTGGTAAGACATTTTATGTGTTCAGAACATAAGCAATGGAAACGTACTACCATAAAAAATGTTTACAATATGAAGAAGCTGGCGGCAGCAGGAGTGAACTTTGAACCAATAAAAGAAGATGCACGCCTTTTCATTGAACGAGATAAGGAAGCTGCTAGTAAATGTAAGTTGAACTTTACATGTTTTAATAATATGAACCTGAAGCTCACAGAATTACGTGTAAAGGATGACACAGAATGTATTGTTCGAAATGTTATGGCCTTGGAGCAGTTTGTGTACCCGAAGGAGACGTATGTCTGTAATTACTTTCTGCTAATGGATCAGCTTGTGGACACTGTGGAAGATGTCGATTTTTTGGTTGCCAGAAAAGTTATCGTTAACATGCTAGGCAGCAATGAAGCAGCGGCAAATATGATTAATGGACTTTGTGATCATATCATGGAAGAAAGCTCCTGCTATGTTGACATCTGTGAGGACCTTAACAAGCACTACGAGAGTTCTTGGAACCGTCATATTGCAACCCTGAAAAGAGTTTATTTCAAGGATCTTTGGACAGCCAGTTCAACTATACTCGGATCTGCTGTCCTGGTTTTCTCAATCATTGGATCCATTCAATCATTCAAAAGCTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

441

Amino Acids

51.51

Weight (kDa)

6.3

Isoelectric Point (pI)

46.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 9 - 428 2.6e-92 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 152
AciI CCGC 4 cut(s) 49, 235, 776, 1097
AclWI GGATC 8 cut(s) 269, 282, 699, 1020, 1245, 1276, 1293, 1306
AcsI RAATTY 3 cut(s) 160, 228, 458
AcuI CTGAAG 1 cut(s) 914
AfaI GTAC 2 cut(s) 739, 971
AflIII ACRYGT 2 cut(s) 872, 910
AgsI TTSAA 8 cut(s) 634, 797, 830, 865, 1234, 1257, 1307, 1315
AhlI ACTAGT 1 cut(s) 662
AjnI CCWGG 2 cut(s) 522, 1278
AloI GAACNNNNNNTCC 2 cut(s) 1237, 1269
Alw21I GWGCWC 1 cut(s) 321
Alw26I GTCTC 1 cut(s) 974
AlwI GGATC 8 cut(s) 269, 282, 699, 1020, 1245, 1276, 1293, 1306
AlwNI CAGNNNCTG 1 cut(s) 406
AoxI GGCC 1 cut(s) 951
ApeKI GCWGC 5 cut(s) 65, 779, 845, 1083, 1094
ApoI RAATTY 3 cut(s) 160, 228, 458
AseI ATTAAT 1 cut(s) 1110
Asp700I GAANNNNTTC 2 cut(s) 228, 683
AspS9I GGNCC 4 cut(s) 214, 448, 500, 1168
AsuHPI GGTGA 2 cut(s) 366, 551
AsuII TTCGAA 2 cut(s) 417, 940
AvaII GGWCC 4 cut(s) 214, 448, 500, 1168
BamHI GGATCC 2 cut(s) 274, 1298
BanII GRGCYC 1 cut(s) 321
Bbv12I GWGCWC 1 cut(s) 321
BbvCI CCTCAGC 1 cut(s) 383
BbvI GCAGC 5 cut(s) 77, 791, 832, 1095, 1106
BccI CCATC 2 cut(s) 98, 272
BciT130I CCWGG 2 cut(s) 524, 1280
BclI TGATCA 2 cut(s) 541, 1123
BcoDI GTCTC 1 cut(s) 974
BcuI ACTAGT 1 cut(s) 662
BfaI CTAG 4 cut(s) 39, 663, 849, 1079
BfmI CTRYAG 1 cut(s) 28
BisI GCNGC 7 cut(s) 66, 777, 780, 846, 1084, 1095, 1098
BlpI GCTNAGC 1 cut(s) 465
BlsI GCNGC 7 cut(s) 67, 778, 781, 847, 1085, 1096, 1099
Bme1390I CCNGG 2 cut(s) 524, 1280
Bme18I GGWCC 4 cut(s) 214, 448, 500, 1168
BmgT120I GGNCC 4 cut(s) 214, 448, 500, 1168
BmiI GGNNCC 5 cut(s) 36, 215, 276, 1199, 1300
BmrFI CCNGG 2 cut(s) 524, 1280
BmsI GCATC 3 cut(s) 289, 574, 802
BoxI GACNNNNGTC 1 cut(s) 986
BpmI CTGGAG 1 cut(s) 143
Bpu10I CCTNAGC 1 cut(s) 383
Bpu1102I GCTNAGC 1 cut(s) 465
Bpu14I TTCGAA 2 cut(s) 417, 940
BpuEI CTTGAG 1 cut(s) 464
BsaAI YACGTR 1 cut(s) 911
BsaBI GATNNNNATC 2 cut(s) 263, 575
BsaJI CCNNGG 4 cut(s) 106, 377, 522, 954
BsaWI WCCGGW 1 cut(s) 10
Bse1I ACTGG 3 cut(s) 126, 593, 1251
Bse3DI GCAATG 3 cut(s) 58, 734, 1093
Bse8I GATNNNNATC 2 cut(s) 263, 575
BseBI CCWGG 2 cut(s) 524, 1280
BseDI CCNNGG 4 cut(s) 106, 377, 522, 954
BseGI GGATG 4 cut(s) 10, 196, 576, 925
BseJI GATNNNNATC 2 cut(s) 263, 575
BseMI GCAATG 3 cut(s) 58, 734, 1093
BseMII CTCAG 3 cut(s) 92, 374, 479
BseNI ACTGG 3 cut(s) 126, 593, 1251
BseRI GAGGAG 1 cut(s) 66
BseXI GCAGC 5 cut(s) 77, 791, 832, 1095, 1106
BshFI GGCC 1 cut(s) 953
BsiHKAI GWGCWC 1 cut(s) 321
BsiSI CCGG 1 cut(s) 11
BslFI GGGAC 3 cut(s) 227, 262, 537
BsmAI GTCTC 1 cut(s) 974
BsmBI CGTCTC 1 cut(s) 974
BsmFI GGGAC 3 cut(s) 227, 262, 537
BsnI GGCC 1 cut(s) 953
Bsp119I TTCGAA 2 cut(s) 417, 940
Bsp1286I GDGCHC 1 cut(s) 321
Bsp1720I GCTNAGC 1 cut(s) 465
Bsp19I CCATGG 2 cut(s) 106, 377
BspACI CCGC 4 cut(s) 49, 235, 776, 1097
BspANI GGCC 1 cut(s) 953
BspCNI CTCAG 3 cut(s) 91, 375, 478
BspHI TCATGA 1 cut(s) 349
BspLI GGNNCC 5 cut(s) 36, 215, 276, 1199, 1300
BspPI GGATC 8 cut(s) 269, 282, 699, 1020, 1245, 1276, 1293, 1306
BspT104I TTCGAA 2 cut(s) 417, 940
BsrDI GCAATG 3 cut(s) 58, 734, 1093
BsrI ACTGG 3 cut(s) 126, 593, 1251
BssECI CCNNGG 4 cut(s) 106, 377, 522, 954
BssNAI GTATAC 1 cut(s) 153
BssT1I CCWWGG 3 cut(s) 106, 377, 954
Bst1107I GTATAC 1 cut(s) 153
Bst2UI CCWGG 2 cut(s) 524, 1280
Bst4CI ACNGT 4 cut(s) 218, 551, 1030, 1202
Bst6I CTCTTC 2 cut(s) 104, 444
BstBAI YACGTR 1 cut(s) 911
BstBI TTCGAA 2 cut(s) 417, 940
BstC8I GCNNGC 5 cut(s) 304, 618, 622, 774, 817
BstDEI CTNAG 3 cut(s) 78, 383, 465
BstDSI CCRYGG 2 cut(s) 106, 377
BstF5I GGATG 4 cut(s) 10, 196, 576, 925
BstMAI GTCTC 1 cut(s) 974
BstMWI GCNNNNNNNGC 2 cut(s) 48, 325
BstNI CCWGG 2 cut(s) 524, 1280
BstNSI RCATGY 2 cut(s) 876, 1078
BstPAI GACNNNNGTC 1 cut(s) 986
BstSCI CCNGG 2 cut(s) 522, 1278
BstSFI CTRYAG 1 cut(s) 28
BstV1I GCAGC 5 cut(s) 77, 791, 832, 1095, 1106
BstX2I RGATCY 5 cut(s) 274, 691, 1237, 1268, 1298
BstYI RGATCY 5 cut(s) 274, 691, 1237, 1268, 1298
BstZ17I GTATAC 1 cut(s) 153
BsuRI GGCC 1 cut(s) 953
BtgI CCRYGG 2 cut(s) 106, 377
BtsCI GGATG 4 cut(s) 10, 196, 576, 925
BtsIMutI CAGTG 1 cut(s) 1026
Cac8I GCNNGC 5 cut(s) 304, 618, 622, 774, 817
CaiI CAGNNNCTG 1 cut(s) 406
CciI TCATGA 1 cut(s) 349
Cfr13I GGNCC 4 cut(s) 214, 448, 500, 1168
Csp6I GTAC 2 cut(s) 738, 970
CviAII CATG 8 cut(s) 107, 130, 350, 378, 581, 873, 1075, 1132
CviQI GTAC 2 cut(s) 738, 970
DdeI CTNAG 3 cut(s) 78, 383, 465
Eam1104I CTCTTC 2 cut(s) 104, 444
EarI CTCTTC 2 cut(s) 104, 444
Ecl136II GAGCTC 1 cut(s) 319
Eco130I CCWWGG 3 cut(s) 106, 377, 954
Eco24I GRGCYC 1 cut(s) 321
Eco47I GGWCC 4 cut(s) 214, 448, 500, 1168
Eco53kI GAGCTC 1 cut(s) 319
Eco57I CTGAAG 1 cut(s) 914
EcoICRI GAGCTC 1 cut(s) 319
EcoO109I RGGNCCY 2 cut(s) 448, 1168
EcoRII CCWGG 2 cut(s) 522, 1278
EcoT14I CCWWGG 3 cut(s) 106, 377, 954
EcoT38I GRGCYC 1 cut(s) 321
ErhI CCWWGG 3 cut(s) 106, 377, 954
Esp3I CGTCTC 1 cut(s) 974
FaeI CATG 8 cut(s) 110, 133, 353, 381, 584, 876, 1078, 1135
FalI AAGNNNNNCTT 2 cut(s) 851, 883
FaqI GGGAC 3 cut(s) 227, 262, 537
FatI CATG 8 cut(s) 106, 129, 349, 377, 580, 872, 1074, 1131
FbaI TGATCA 2 cut(s) 541, 1123
FblI GTMKAC 1 cut(s) 152
Fnu4HI GCNGC 7 cut(s) 66, 777, 780, 846, 1084, 1095, 1098
FokI GGATG 4 cut(s) 17, 203, 563, 932
FriOI GRGCYC 1 cut(s) 321
Fsp4HI GCNGC 7 cut(s) 66, 777, 780, 846, 1084, 1095, 1098
FspBI CTAG 4 cut(s) 39, 663, 849, 1079
GluI GCNGC 7 cut(s) 66, 777, 780, 846, 1084, 1095, 1098
GsuI CTGGAG 1 cut(s) 143
HaeIII GGCC 1 cut(s) 953
HapII CCGG 1 cut(s) 11
Hin1II CATG 8 cut(s) 110, 133, 353, 381, 584, 876, 1078, 1135
HincII GTYRAC 2 cut(s) 1072, 1156
HindII GTYRAC 2 cut(s) 1072, 1156
HindIII AAGCTT 1 cut(s) 1318
HinfI GANTC 4 cut(s) 96, 259, 346, 560
HpaI GTTAAC 1 cut(s) 1072
HpaII CCGG 1 cut(s) 11
HphI GGTGA 2 cut(s) 366, 551
Hpy166II GTNNAC 8 cut(s) 153, 479, 757, 790, 970, 1024, 1072, 1156
Hpy188I TCNGA 3 cut(s) 95, 718, 1268
Hpy188III TCNNGA 4 cut(s) 256, 350, 443, 545
Hpy8I GTNNAC 8 cut(s) 153, 479, 757, 790, 970, 1024, 1072, 1156
HpyAV CCTTC 1 cut(s) 970
HpyCH4III ACNGT 4 cut(s) 218, 551, 1030, 1202
HpyCH4IV ACGT 3 cut(s) 736, 910, 983
HpyCH4V TGCA 3 cut(s) 245, 815, 1211
HpyF10VI GCNNNNNNNGC 2 cut(s) 48, 325
HpyF3I CTNAG 3 cut(s) 78, 383, 465
HpySE526I ACGT 3 cut(s) 736, 910, 983
Hsp92II CATG 8 cut(s) 110, 133, 353, 381, 584, 876, 1078, 1135
Ksp22I TGATCA 2 cut(s) 541, 1123
KspAI GTTAAC 1 cut(s) 1072
LmnI GCTCC 4 cut(s) 324, 613, 958, 1148
Lsp1109I GCAGC 5 cut(s) 77, 791, 832, 1095, 1106
LweI GCATC 3 cut(s) 289, 574, 802
MaeI CTAG 4 cut(s) 39, 663, 849, 1079
MaeII ACGT 3 cut(s) 736, 910, 983
MaeIII GTNAC 1 cut(s) 81
MboII GAAGA 7 cut(s) 91, 431, 622, 778, 821, 1046, 1148
MflI RGATCY 5 cut(s) 274, 691, 1237, 1268, 1298
MhlI GDGCHC 1 cut(s) 321
MluCI AATT 7 cut(s) 160, 228, 288, 458, 604, 905, 994
MlyI GAGTC 1 cut(s) 90
MnlI CCTC 6 cut(s) 87, 118, 378, 439, 640, 1159
MroXI GAANNNNTTC 2 cut(s) 228, 683
MseI TTAA 5 cut(s) 368, 879, 1071, 1110, 1173
MslI CAYNNNNRTG 1 cut(s) 354
MspA1I CMGCKG 1 cut(s) 1097
MspI CCGG 1 cut(s) 11
MspR9I CCNGG 2 cut(s) 524, 1280
MvaI CCWGG 2 cut(s) 524, 1280
MwoI GCNNNNNNNGC 2 cut(s) 48, 325
NcoI CCATGG 2 cut(s) 106, 377
NlaIII CATG 8 cut(s) 110, 133, 353, 381, 584, 876, 1078, 1135
NlaIV GGNNCC 5 cut(s) 36, 215, 276, 1199, 1300
NspI RCATGY 2 cut(s) 876, 1078
NspV TTCGAA 2 cut(s) 417, 940
PagI TCATGA 1 cut(s) 349
PciI ACATGT 1 cut(s) 872
PdmI GAANNNNTTC 2 cut(s) 228, 683
PfeI GAWTC 3 cut(s) 259, 346, 560
PkrI GCNGC 7 cut(s) 67, 778, 781, 847, 1085, 1096, 1099
PleI GAGTC 1 cut(s) 90
PpsI GAGTC 1 cut(s) 90
Ppu21I YACGTR 1 cut(s) 911
PpuMI RGGWCCY 2 cut(s) 448, 1168
PscI ACATGT 1 cut(s) 872
PshAI GACNNNNGTC 1 cut(s) 986
PshBI ATTAAT 1 cut(s) 1110
Psp124BI GAGCTC 1 cut(s) 321
Psp5II RGGWCCY 2 cut(s) 448, 1168
Psp6I CCWGG 2 cut(s) 522, 1278
PspGI CCWGG 2 cut(s) 522, 1278
PspN4I GGNNCC 5 cut(s) 36, 215, 276, 1199, 1300
PspPI GGNCC 4 cut(s) 214, 448, 500, 1168
PspPPI RGGWCCY 2 cut(s) 448, 1168
PstNI CAGNNNCTG 1 cut(s) 406
PsuI RGATCY 5 cut(s) 274, 691, 1237, 1268, 1298
RsaI GTAC 2 cut(s) 739, 971
RsaNI GTAC 2 cut(s) 738, 970
RseI CAYNNNNRTG 1 cut(s) 354
SacI GAGCTC 1 cut(s) 321
SaqAI TTAA 5 cut(s) 368, 879, 1071, 1110, 1173
SatI GCNGC 7 cut(s) 66, 777, 780, 846, 1084, 1095, 1098
Sau96I GGNCC 4 cut(s) 214, 448, 500, 1168
SchI GAGTC 1 cut(s) 90
ScrFI CCNGG 2 cut(s) 524, 1280
SduI GDGCHC 1 cut(s) 321
SfaNI GCATC 3 cut(s) 289, 574, 802
SfcI CTRYAG 1 cut(s) 28
SfuI TTCGAA 2 cut(s) 417, 940
SinI GGWCC 4 cut(s) 214, 448, 500, 1168
SmiMI CAYNNNNRTG 1 cut(s) 354
SmlI CTYRAG 1 cut(s) 443
SmoI CTYRAG 1 cut(s) 443
SpeI ACTAGT 1 cut(s) 662
Sse9I AATT 7 cut(s) 160, 228, 288, 458, 604, 905, 994
SsiI CCGC 4 cut(s) 49, 235, 776, 1097
SspMI CTAG 4 cut(s) 39, 663, 849, 1079
SstI GAGCTC 1 cut(s) 321
StyD4I CCNGG 2 cut(s) 522, 1278
StyI CCWWGG 3 cut(s) 106, 377, 954
TaaI ACNGT 4 cut(s) 218, 551, 1030, 1202
TaiI ACGT 3 cut(s) 739, 913, 986
TaqI TCGA 4 cut(s) 267, 417, 940, 1041
TasI AATT 7 cut(s) 160, 228, 288, 458, 604, 905, 994
TauI GCSGC 2 cut(s) 779, 1100
TfiI GAWTC 3 cut(s) 259, 346, 560
Tru1I TTAA 5 cut(s) 368, 879, 1071, 1110, 1173
Tru9I TTAA 5 cut(s) 368, 879, 1071, 1110, 1173
TscAI CASTG 1 cut(s) 1033
TseI GCWGC 5 cut(s) 65, 779, 845, 1083, 1094
TspGWI ACGGA 2 cut(s) 464, 704
TspRI CASTG 1 cut(s) 1033
VpaK11BI GGWCC 4 cut(s) 214, 448, 500, 1168
VspI ATTAAT 1 cut(s) 1110
XapI RAATTY 3 cut(s) 160, 228, 458
XceI RCATGY 2 cut(s) 876, 1078
XmiI GTMKAC 1 cut(s) 152
XmnI GAANNNNTTC 2 cut(s) 228, 683
XspI CTAG 4 cut(s) 39, 663, 849, 1079
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.