MD00G1041300.v1.1

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0000821
Physical Location & Seq
Reverse (-)
7344369 .. 7346385
2017 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1041300.v1.1.491

Sequence Viewer

Length: 1548 bp
ATGCATGTCATCTACTTACCACTAAACTTCTTTTTATCCTGCCAGGCCCAAAATTTTCATCAGGGGTGCAAGCACGAATTATCGCCGGAAGAACCTAAGACAACACCACAAATTGCGGAGACAAATGATCGCGAACAAGATTTCCATGACCATGTACTAGTCTTGGACCAGCCAGACAAGTGGTGTATCTACAGGGTCCCCAGCAAACTTCGCAAAGTAAACGAAGCAGCATACACTCCTCAGTTACTATCAATCGGCCTTTTCCACCATGGCAAGCCGGAACTCAAGGACATGGAGACCCACAAAAATATGTACTACAAGAATTTTTCCGCGCGTTGTTTGAAGAAGGACGTTGCACTAAAAAAATTCATCAAGGCTCGTCAAGAACACATTCTTCGTTGTTATGCAGGGGCCATTGAGCTTGACAAAGATCCTGTTGACATAATTGTGGTCGATGCCTGCTTCATCATTGAGCTCTTTTTGATGAACTCTGAAGAAACAGAAAATCATGAAAACGATTACATTTTAAGATCACCGTGGCTGAGGAAAGCTGTAGAGCAGGAACTAATACTGTTTGAAAACCAGCTTCCGTATTCTCTTCTTCAAGAGCTATATGACTTTGCGGTGCCTGCCTCCTGTTCCCCTCATTCCAAAGAAGCACAGGAACATGAACAGGCTCATAGAAACAGCAGTGCCAATCATGACCAGCAGGACTGCTTGCCATGCTACCCTCTGGTCCGCTTCGGTGATCTTTGTATAGATGTTCCAAAAGCCGAACCTGTCCCTGATTGTATAGATGTTCCAAAAGCCGAACCTGTCCCTGATCATCCCTTTCTTAAGCTTACCTGCGAGTTCTTCAAAGATTACAGCAAGGGAAAATCCGTGAATGGGGTAAAACCAAAACACTTCACTGATTTGGTAAGACATTTTCTATGTCCCCAAGAAGAAATGACATTTGAACATAATACTTGTCCTATAAAAAATATATATGCTGTAAGGAAGCTGAGGGCAGCAGGGGTGAAGTTTACGCCACTTCGAGAAGACGGATACTTTATCATAAAAGGAGACAAGCCTACTAAATGCAAGTTGAACTTGGCATGTTTTCGAAATATGAACTTGAAGCTTGCACAGTTTTGTGTCAAGGATGAGACAGAGTGCGTTATTCGAAACATCATGGCCTTGGAGCAGTTTTTGTACCCGGACAAGCCTTATATCTGCAATTACTTTATGCTCATGGATCAACTTGTGGACACTGTAGAAGATGTTGATTTGCTGGTTGAGAACAAAGTTATTGTTAACATGCTGGGCAGCAACGAAGCAGTGGCAAAGCTGGTTAATAGACTTTGCGAGCAGATTATGGAGGATAAATCCTGCTATGCTGACATCTGTAAACAGCTTAACAGGCACTATGAGAATTTTTGGAATCGTCATGTGGCGACCCTGAAACGAGTTTACTTCAAGGACCTTTGGACAGGCAGTTCGACTATACTGGGACTTTTCGTCCTCGTTTTCTCCATCATTGGAAGCATAAAGTCGCTCAAATCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

516

Amino Acids

59.74

Weight (kDa)

6.22

Isoelectric Point (pI)

42.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 63 - 504 1.7e-92 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 854
AccB1I GGYRCC 1 cut(s) 625
AccII CGCG 3 cut(s) 132, 332, 334
AciI CCGC 4 cut(s) 116, 330, 623, 739
AclWI GGATC 2 cut(s) 425, 1245
AcsI RAATTY 4 cut(s) 52, 322, 365, 1414
AcuI CTGAAG 1 cut(s) 513
AfaI GTAC 3 cut(s) 156, 314, 1196
AfiI CCNNNNNNNGG 1 cut(s) 888
AflII CTTAAG 1 cut(s) 836
AgsI TTSAA 8 cut(s) 343, 578, 605, 859, 959, 1090, 1120, 1459
AhdI GACNNNNNGTC 1 cut(s) 1499
AhlI ACTAGT 1 cut(s) 157
AjnI CCWGG 1 cut(s) 42
AloI GAACNNNNNNTCC 4 cut(s) 126, 158, 1462, 1494
Alw21I GWGCWC 1 cut(s) 477
Alw26I GTCTC 4 cut(s) 113, 290, 1059, 1142
AlwI GGATC 2 cut(s) 425, 1245
AoxI GGCC 4 cut(s) 45, 256, 411, 1176
ApeKI GCWGC 3 cut(s) 227, 1010, 1308
ApoI RAATTY 4 cut(s) 52, 322, 365, 1414
ArsI GACNNNNNNTTYG 2 cut(s) 1462, 1494
Asp700I GAANNNNTTC 1 cut(s) 390
AspLEI GCGC 1 cut(s) 334
AspS9I GGNCC 6 cut(s) 46, 166, 196, 411, 736, 1462
AsuC2I CCSGG 1 cut(s) 1199
AsuHPI GGTGA 3 cut(s) 525, 758, 1030
AsuII TTCGAA 2 cut(s) 1105, 1165
AvaII GGWCC 4 cut(s) 166, 196, 736, 1462
BanI GGYRCC 1 cut(s) 625
BanII GRGCYC 1 cut(s) 477
BbsI GAAGAC 1 cut(s) 1047
Bbv12I GWGCWC 1 cut(s) 477
BbvCI CCTCAGC 2 cut(s) 542, 1004
BbvI GCAGC 3 cut(s) 239, 1022, 1320
BccI CCATC 1 cut(s) 1523
BciT130I CCWGG 1 cut(s) 44
BciVI GTATCC 1 cut(s) 1040
BclI TGATCA 1 cut(s) 823
BcnI CCSGG 1 cut(s) 1199
BcoDI GTCTC 4 cut(s) 113, 290, 1059, 1142
BcuI ACTAGT 1 cut(s) 157
BfaI CTAG 1 cut(s) 158
BfmI CTRYAG 3 cut(s) 190, 552, 1254
BfrI CTTAAG 1 cut(s) 836
BfuAI ACCTGC 1 cut(s) 854
BfuI GTATCC 1 cut(s) 1040
BisI GCNGC 3 cut(s) 228, 1011, 1309
BlsI GCNGC 3 cut(s) 229, 1012, 1310
Bme1390I CCNGG 2 cut(s) 44, 1199
Bme18I GGWCC 4 cut(s) 166, 196, 736, 1462
BmeRI GACNNNNNGTC 1 cut(s) 1499
BmgT120I GGNCC 6 cut(s) 46, 166, 196, 411, 736, 1462
BmiI GGNNCC 4 cut(s) 197, 198, 412, 627
BmrFI CCNGG 2 cut(s) 44, 1199
BmrI ACTGGG 1 cut(s) 1499
BmsI GCATC 1 cut(s) 445
BmuI ACTGGG 1 cut(s) 1499
BpiI GAAGAC 1 cut(s) 1047
Bpu10I CCTNAGC 2 cut(s) 542, 1004
Bpu14I TTCGAA 2 cut(s) 1105, 1165
BpuEI CTTGAG 1 cut(s) 269
BpuMI CCSGG 1 cut(s) 1199
BsaI GGTCTC 1 cut(s) 290
BsaJI CCNNGG 3 cut(s) 268, 536, 1179
Bsc4I CCNNNNNNNGG 1 cut(s) 888
Bse1I ACTGG 1 cut(s) 1494
BseBI CCWGG 1 cut(s) 44
BseDI CCNNGG 3 cut(s) 268, 536, 1179
BseGI GGATG 2 cut(s) 826, 1150
BseLI CCNNNNNNNGG 1 cut(s) 888
BseMII CTCAG 3 cut(s) 254, 533, 995
BseNI ACTGG 1 cut(s) 1494
BseRI GAGGAG 1 cut(s) 228
BseXI GCAGC 3 cut(s) 239, 1022, 1320
BseYI CCCAGC 2 cut(s) 200, 1303
Bsh1236I CGCG 3 cut(s) 132, 332, 334
BshFI GGCC 4 cut(s) 47, 258, 413, 1178
BshNI GGYRCC 1 cut(s) 625
BsiHKAI GWGCWC 1 cut(s) 477
BsiSI CCGG 3 cut(s) 86, 278, 1199
BslFI GGGAC 5 cut(s) 182, 767, 803, 921, 1506
BslI CCNNNNNNNGG 1 cut(s) 888
BsmAI GTCTC 4 cut(s) 113, 290, 1059, 1142
BsmFI GGGAC 5 cut(s) 182, 767, 803, 921, 1506
BsnI GGCC 4 cut(s) 47, 258, 413, 1178
Bso31I GGTCTC 1 cut(s) 290
Bsp119I TTCGAA 2 cut(s) 1105, 1165
Bsp1286I GDGCHC 1 cut(s) 477
Bsp143I GATC 6 cut(s) 127, 430, 530, 748, 823, 1237
Bsp19I CCATGG 1 cut(s) 268
Bsp68I TCGCGA 1 cut(s) 132
BspACI CCGC 4 cut(s) 116, 330, 623, 739
BspANI GGCC 4 cut(s) 47, 258, 413, 1178
BspCNI CTCAG 3 cut(s) 253, 534, 996
BspFNI CGCG 3 cut(s) 132, 332, 334
BspHI TCATGA 2 cut(s) 508, 700
BspLI GGNNCC 4 cut(s) 197, 198, 412, 627
BspMI ACCTGC 1 cut(s) 854
BspPI GGATC 2 cut(s) 425, 1245
BspT104I TTCGAA 2 cut(s) 1105, 1165
BspT107I GGYRCC 1 cut(s) 625
BspTI CTTAAG 1 cut(s) 836
BspTNI GGTCTC 1 cut(s) 290
BsrI ACTGG 1 cut(s) 1494
BssECI CCNNGG 3 cut(s) 268, 536, 1179
BssMI GATC 6 cut(s) 127, 430, 530, 748, 823, 1237
BssT1I CCWWGG 2 cut(s) 268, 1179
Bst2UI CCWGG 1 cut(s) 44
Bst4CI ACNGT 4 cut(s) 537, 573, 1131, 1255
Bst6I CTCTTC 1 cut(s) 603
BstAFI CTTAAG 1 cut(s) 836
BstBI TTCGAA 2 cut(s) 1105, 1165
BstC8I GCNNGC 7 cut(s) 71, 275, 460, 630, 719, 1125, 1349
BstDEI CTNAG 4 cut(s) 96, 240, 542, 1004
BstDSI CCRYGG 2 cut(s) 268, 536
BstF5I GGATG 2 cut(s) 826, 1150
BstFNI CGCG 3 cut(s) 132, 332, 334
BstHHI GCGC 1 cut(s) 334
BstKTI GATC 6 cut(s) 130, 433, 533, 751, 826, 1240
BstMAI GTCTC 4 cut(s) 113, 290, 1059, 1142
BstMBI GATC 6 cut(s) 127, 430, 530, 748, 823, 1237
BstMWI GCNNNNNNNGC 4 cut(s) 210, 629, 723, 1402
BstNI CCWGG 1 cut(s) 44
BstNSI RCATGY 3 cut(s) 8, 1101, 1303
BstSCI CCNGG 2 cut(s) 42, 1197
BstSFI CTRYAG 3 cut(s) 190, 552, 1254
BstUI CGCG 3 cut(s) 132, 332, 334
BstV1I GCAGC 3 cut(s) 239, 1022, 1320
BstV2I GAAGAC 1 cut(s) 1047
BstX2I RGATCY 1 cut(s) 430
BstXI CCANNNNNNTGG 1 cut(s) 180
BstYI RGATCY 1 cut(s) 430
BsuI GTATCC 1 cut(s) 1040
BsuRI GGCC 4 cut(s) 47, 258, 413, 1178
BtgI CCRYGG 2 cut(s) 268, 536
BtsCI GGATG 2 cut(s) 826, 1150
BtsI GCAGTG 2 cut(s) 697, 1326
BtsIMutI CAGTG 4 cut(s) 697, 909, 1251, 1326
BtuMI TCGCGA 1 cut(s) 132
BveI ACCTGC 1 cut(s) 854
Cac8I GCNNGC 7 cut(s) 71, 275, 460, 630, 719, 1125, 1349
CciI TCATGA 2 cut(s) 508, 700
CfoI GCGC 1 cut(s) 334
Cfr13I GGNCC 6 cut(s) 46, 166, 196, 411, 736, 1462
Csp6I GTAC 3 cut(s) 155, 313, 1195
CviQI GTAC 3 cut(s) 155, 313, 1195
DdeI CTNAG 4 cut(s) 96, 240, 542, 1004
DpnI GATC 6 cut(s) 129, 432, 532, 750, 825, 1239
DpnII GATC 6 cut(s) 127, 430, 530, 748, 823, 1237
DriI GACNNNNNGTC 1 cut(s) 1499
Eam1104I CTCTTC 1 cut(s) 603
Eam1105I GACNNNNNGTC 1 cut(s) 1499
EarI CTCTTC 1 cut(s) 603
Ecl136II GAGCTC 1 cut(s) 475
Eco130I CCWWGG 2 cut(s) 268, 1179
Eco24I GRGCYC 1 cut(s) 477
Eco31I GGTCTC 1 cut(s) 290
Eco47I GGWCC 4 cut(s) 166, 196, 736, 1462
Eco53kI GAGCTC 1 cut(s) 475
Eco57I CTGAAG 1 cut(s) 513
EcoICRI GAGCTC 1 cut(s) 475
EcoO109I RGGNCCY 2 cut(s) 196, 1462
EcoRII CCWGG 1 cut(s) 42
EcoT14I CCWWGG 2 cut(s) 268, 1179
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 1 cut(s) 477
ErhI CCWWGG 2 cut(s) 268, 1179
FalI AAGNNNNNCTT 2 cut(s) 1076, 1108
FaqI GGGAC 5 cut(s) 182, 767, 803, 921, 1506
FbaI TGATCA 1 cut(s) 823
Fnu4HI GCNGC 3 cut(s) 228, 1011, 1309
FokI GGATG 2 cut(s) 813, 1157
FriOI GRGCYC 1 cut(s) 477
Fsp4HI GCNGC 3 cut(s) 228, 1011, 1309
FspBI CTAG 1 cut(s) 158
GlaI GCGC 1 cut(s) 333
GluI GCNGC 3 cut(s) 228, 1011, 1309
GsaI CCCAGC 2 cut(s) 204, 1307
HaeIII GGCC 4 cut(s) 47, 258, 413, 1178
HapII CCGG 3 cut(s) 86, 278, 1199
HhaI GCGC 1 cut(s) 334
Hin6I GCGC 1 cut(s) 332
HinP1I GCGC 1 cut(s) 332
HincII GTYRAC 2 cut(s) 439, 1297
HindII GTYRAC 2 cut(s) 439, 1297
HindIII AAGCTT 2 cut(s) 839, 1121
HinfI GANTC 1 cut(s) 1423
HpaI GTTAAC 1 cut(s) 1297
HpaII CCGG 3 cut(s) 86, 278, 1199
HphI GGTGA 3 cut(s) 525, 758, 1030
Hpy166II GTNNAC 7 cut(s) 220, 439, 1026, 1249, 1297, 1391, 1453
Hpy188I TCNGA 1 cut(s) 493
Hpy188III TCNNGA 6 cut(s) 131, 383, 509, 605, 701, 1037
Hpy8I GTNNAC 7 cut(s) 220, 439, 1026, 1249, 1297, 1391, 1453
HpyAV CCTTC 1 cut(s) 340
HpyCH4III ACNGT 4 cut(s) 537, 573, 1131, 1255
HpyCH4IV ACGT 1 cut(s) 351
HpyCH4V TGCA 7 cut(s) 4, 69, 356, 407, 1083, 1127, 1218
HpyF10VI GCNNNNNNNGC 4 cut(s) 210, 629, 723, 1402
HpyF3I CTNAG 4 cut(s) 96, 240, 542, 1004
HpySE526I ACGT 1 cut(s) 351
HspAI GCGC 1 cut(s) 332
KflI GGGWCCC 1 cut(s) 196
Ksp22I TGATCA 1 cut(s) 823
KspAI GTTAAC 1 cut(s) 1297
Kzo9I GATC 6 cut(s) 127, 430, 530, 748, 823, 1237
LmnI GCTCC 1 cut(s) 1183
Lsp1109I GCAGC 3 cut(s) 239, 1022, 1320
LweI GCATC 1 cut(s) 445
MaeI CTAG 1 cut(s) 158
MaeII ACGT 1 cut(s) 351
MaeIII GTNAC 1 cut(s) 243
MalI GATC 6 cut(s) 129, 432, 532, 750, 825, 1239
MboI GATC 6 cut(s) 127, 430, 530, 748, 823, 1237
MflI RGATCY 1 cut(s) 430
MhlI GDGCHC 1 cut(s) 477
MluCI AATT 8 cut(s) 52, 77, 111, 322, 365, 444, 1219, 1414
MnlI CCTC 8 cut(s) 249, 537, 643, 654, 741, 999, 1354, 1514
Mph1103I ATGCAT 1 cut(s) 6
MroXI GAANNNNTTC 1 cut(s) 390
MseI TTAA 5 cut(s) 527, 837, 1296, 1335, 1398
MslI CAYNNNNRTG 2 cut(s) 150, 446
MspCI CTTAAG 1 cut(s) 836
MspI CCGG 3 cut(s) 86, 278, 1199
MspR9I CCNGG 2 cut(s) 44, 1199
MvaI CCWGG 1 cut(s) 44
MvnI CGCG 3 cut(s) 132, 332, 334
MwoI GCNNNNNNNGC 4 cut(s) 210, 629, 723, 1402
NciI CCSGG 1 cut(s) 1199
NcoI CCATGG 1 cut(s) 268
NdeII GATC 6 cut(s) 127, 430, 530, 748, 823, 1237
NlaIV GGNNCC 4 cut(s) 197, 198, 412, 627
NruI TCGCGA 1 cut(s) 132
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 3 cut(s) 8, 1101, 1303
NspV TTCGAA 2 cut(s) 1105, 1165
PagI TCATGA 2 cut(s) 508, 700
PdmI GAANNNNTTC 1 cut(s) 390
PfeI GAWTC 1 cut(s) 1423
PkrI GCNGC 3 cut(s) 229, 1012, 1310
PpuMI RGGWCCY 2 cut(s) 196, 1462
Psp124BI GAGCTC 1 cut(s) 477
Psp5II RGGWCCY 2 cut(s) 196, 1462
Psp6I CCWGG 1 cut(s) 42
PspFI CCCAGC 2 cut(s) 200, 1303
PspGI CCWGG 1 cut(s) 42
PspN4I GGNNCC 4 cut(s) 197, 198, 412, 627
PspPI GGNCC 6 cut(s) 46, 166, 196, 411, 736, 1462
PspPPI RGGWCCY 2 cut(s) 196, 1462
PsuI RGATCY 1 cut(s) 430
RruI TCGCGA 1 cut(s) 132
RsaI GTAC 3 cut(s) 156, 314, 1196
RsaNI GTAC 3 cut(s) 155, 313, 1195
RseI CAYNNNNRTG 2 cut(s) 150, 446
SacI GAGCTC 1 cut(s) 477
SaqAI TTAA 5 cut(s) 527, 837, 1296, 1335, 1398
SatI GCNGC 3 cut(s) 228, 1011, 1309
Sau3AI GATC 6 cut(s) 127, 430, 530, 748, 823, 1237
Sau96I GGNCC 6 cut(s) 46, 166, 196, 411, 736, 1462
ScrFI CCNGG 2 cut(s) 44, 1199
SduI GDGCHC 1 cut(s) 477
SfaNI GCATC 1 cut(s) 445
SfcI CTRYAG 3 cut(s) 190, 552, 1254
SfuI TTCGAA 2 cut(s) 1105, 1165
SinI GGWCC 4 cut(s) 166, 196, 736, 1462
SmiMI CAYNNNNRTG 2 cut(s) 150, 446
SmlI CTYRAG 2 cut(s) 284, 836
SmoI CTYRAG 2 cut(s) 284, 836
SpeI ACTAGT 1 cut(s) 157
Sse9I AATT 8 cut(s) 52, 77, 111, 322, 365, 444, 1219, 1414
SsiI CCGC 4 cut(s) 116, 330, 623, 739
SspMI CTAG 1 cut(s) 158
SstI GAGCTC 1 cut(s) 477
StyD4I CCNGG 2 cut(s) 42, 1197
StyI CCWWGG 2 cut(s) 268, 1179
TaaI ACNGT 4 cut(s) 537, 573, 1131, 1255
TaiI ACGT 1 cut(s) 354
TaqI TCGA 5 cut(s) 453, 1036, 1105, 1165, 1481
TasI AATT 8 cut(s) 52, 77, 111, 322, 365, 444, 1219, 1414
TatI WGTACW 2 cut(s) 154, 312
TfiI GAWTC 1 cut(s) 1423
Tru1I TTAA 5 cut(s) 527, 837, 1296, 1335, 1398
Tru9I TTAA 5 cut(s) 527, 837, 1296, 1335, 1398
TscAI CASTG 4 cut(s) 697, 916, 1258, 1326
TseI GCWGC 3 cut(s) 227, 1010, 1308
TspDTI ATGAA 7 cut(s) 47, 358, 454, 500, 525, 684, 1127
TspGWI ACGGA 3 cut(s) 579, 871, 1059
TspRI CASTG 4 cut(s) 697, 916, 1258, 1326
Vha464I CTTAAG 1 cut(s) 836
VpaK11BI GGWCC 4 cut(s) 166, 196, 736, 1462
XapI RAATTY 4 cut(s) 52, 322, 365, 1414
XceI RCATGY 3 cut(s) 8, 1101, 1303
XmnI GAANNNNTTC 1 cut(s) 390
XspI CTAG 1 cut(s) 158
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.