Prupe.2G125400_v2.0.a1

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0000821
Physical Location & Seq
Forward (+)
18213173 .. 18215599
2427 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G125400.2

Sequence Viewer

Length: 1482 bp
ATGGTTGACGAGAATGCGGACAACAGTGGACAAGAGTCCACCTTAATGTATCATTATCTCAGCGACAGAAGGACAAAGAGTACGTCACAAATCACTGAGAAAATAGAGAATGGAGAAGATTGCCAGGTACAAACTTTGGACACGCCAGACAAGTGGTGTATCTATCAGGTTCCCAGCAAACTCCGCAAAGTAAATGAAGCAGCATACACTCCTCAATTACTATCAATAGGCCCTTTCCACCATGGCAAACCAGAACTGAACAACATGGAGATCCATAAAAGGATATGTTATAAGAAGTTTCTTGAACGTTGTAAGAAGACTGAGGATGATTTAAAGCAATTTATCAACAAGCGTAAAAAAGAAGTTCTTAGTTGTTACGCAGGGACCATTAAGCTTTCCATAATCTCTGCAGACATAATTGCGGTTGATGCCTGCTTCATCATCGAGCTCTTTTTAAGGAACTACGAAAATCATGAAAATGATTATATATTGAGTTCACCATGGCTGAGGAAAGCTGTAGAGCAGGACTTGATACTGATTGAAAATCAGCTTCCTTATTTTCTTCTTCAAGAACTATATCAGAACTTTCCTGTGCATGTCTCTCCCCGCAAAAAAGAAGAAGAAGAACCGACTCATAGACAGAGCAGTACCAATGACTTCCAGTGCTGCTTGCCTTGCTCCTGGCGGATACCTTCCAATGATCTTTCCATAGAGATTGACAAAGCCAAACCTGCTGATGACGAAGCCAATCTTGCTCATTCTGTTCTCCTGCTTGAGCTTACCTGTGAGTTCTTCAAAGAACATACTAAGGGGAAATCCGTCGAGAATGGAGTACCTCAGCCGAAACATTTCACCGATTTGGTTAGGTATTTTCTATGTCCTGACGAACCAATGTCTTGTGAACATGGTGTCGAAAATATATATGCTGCAAAGAAGCTGAGGGCATCAGGGGTGAAGTTTACGCCACTTAAAAAGAAAACCTTCTTGACCATAGAGACAGATAAGGCCACTAAAGGTAAGCCCAACTTAGCATGTTTTAGTAATCTGAACTTGAAGCTTACGCCATTTAAGTTCAAGGATGAGACAGAATGGGTTGTTCGGAACATCATGGCCTTGGAGCAGTTTTTGTATCCAAACAATCCTTATATCTGCAATTACTTTTTGCTTATGGATCAGCTGGTGGACACTGTGGATGATGTGGATTTGCTGGTTGAGAACGAAGTAATTATTAACATGCTAGGCAGCAACAAAGCAGTGGCGAAGCTGGTTAATAGACTTTGCCAGCAGATCAACGAAGATAAATTCTGCTATTCTCATATCGGTCGACAGCTTAAAGTTCACTATGACAATATTTGGAACCGTAATGTGGCAACCCTGAAACGAGTTTACTTCAAGGATCTCTGGACGGGCAGTTCAACTGTACTTGGAGTTTTCGTCCTGCTTTTCTCGGTCATTGGAACCATTAAGTCTCTCAAGTCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

494

Amino Acids

57.01

Weight (kDa)

6.52

Isoelectric Point (pI)

41.28

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 291
Acc36I ACCTGC 1 cut(s) 739
AccI GTMKAC 1 cut(s) 1324
AciI CCGC 5 cut(s) 17, 184, 422, 607, 685
AclI AACGTT 1 cut(s) 307
AclWI GGATC 3 cut(s) 265, 1179, 1404
AcsI RAATTY 1 cut(s) 1301
AfaI GTAC 5 cut(s) 82, 129, 649, 834, 1422
AfiI CCNNNNNNNGG 1 cut(s) 1366
AgsI TTSAA 8 cut(s) 305, 542, 569, 796, 1054, 1075, 1393, 1416
AjnI CCWGG 2 cut(s) 123, 680
AloI GAACNNNNNNTCC 2 cut(s) 1396, 1428
Alw21I GWGCWC 1 cut(s) 450
Alw26I GTCTC 4 cut(s) 604, 989, 1076, 1473
AlwI GGATC 3 cut(s) 265, 1179, 1404
AoxI GGCC 3 cut(s) 229, 1005, 1110
ApeKI GCWGC 4 cut(s) 200, 666, 926, 1242
ApoI RAATTY 1 cut(s) 1301
ArsI GACNNNNNNTTYG 2 cut(s) 68, 100
Asp700I GAANNNNTTC 2 cut(s) 848, 980
AspS9I GGNCC 2 cut(s) 230, 384
AsuHPI GGTGA 3 cut(s) 489, 844, 964
AvaII GGWCC 1 cut(s) 384
BanII GRGCYC 1 cut(s) 450
BbsI GAAGAC 1 cut(s) 323
Bbv12I GWGCWC 1 cut(s) 450
BbvCI CCTCAGC 3 cut(s) 506, 837, 938
BbvI GCAGC 4 cut(s) 212, 653, 913, 1254
BciT130I CCWGG 2 cut(s) 125, 682
BciVI GTATCC 2 cut(s) 681, 1140
BcoDI GTCTC 4 cut(s) 604, 989, 1076, 1473
BfaI CTAG 1 cut(s) 1238
BfmI CTRYAG 2 cut(s) 408, 516
BfuAI ACCTGC 1 cut(s) 739
BfuI GTATCC 2 cut(s) 681, 1140
BisI GCNGC 4 cut(s) 201, 667, 927, 1243
BlsI GCNGC 4 cut(s) 202, 668, 928, 1244
Bme1390I CCNGG 2 cut(s) 125, 682
Bme18I GGWCC 1 cut(s) 384
BmgT120I GGNCC 2 cut(s) 230, 384
BmiI GGNNCC 4 cut(s) 171, 385, 1358, 1459
BmrFI CCNGG 2 cut(s) 125, 682
BmsI GCATC 2 cut(s) 418, 953
BoxI GACNNNNGTC 1 cut(s) 34
BpiI GAAGAC 1 cut(s) 323
Bpu10I CCTNAGC 3 cut(s) 506, 837, 938
BpuEI CTTGAG 2 cut(s) 794, 1457
BsaJI CCNNGG 3 cut(s) 241, 500, 1113
BsaXI ACNNNNNCTCC 2 cut(s) 1419, 1449
Bsc4I CCNNNNNNNGG 1 cut(s) 1366
Bse1I ACTGG 1 cut(s) 661
BseBI CCWGG 2 cut(s) 125, 682
BseDI CCNNGG 3 cut(s) 241, 500, 1113
BseGI GGATG 3 cut(s) 331, 1084, 1198
BseLI CCNNNNNNNGG 1 cut(s) 1366
BseMII CTCAG 6 cut(s) 73, 87, 312, 497, 851, 929
BseNI ACTGG 1 cut(s) 661
BseRI GAGGAG 1 cut(s) 201
BseXI GCAGC 4 cut(s) 212, 653, 913, 1254
BseYI CCCAGC 1 cut(s) 173
Bsh1285I CGRYCG 1 cut(s) 1324
BshFI GGCC 3 cut(s) 231, 1007, 1112
BsiEI CGRYCG 1 cut(s) 1324
BsiHKAI GWGCWC 1 cut(s) 450
BslFI GGGAC 1 cut(s) 397
BslI CCNNNNNNNGG 1 cut(s) 1366
BsmAI GTCTC 4 cut(s) 604, 989, 1076, 1473
BsmFI GGGAC 1 cut(s) 397
BsmI GAATGC 1 cut(s) 19
BsnI GGCC 3 cut(s) 231, 1007, 1112
Bsp1286I GDGCHC 1 cut(s) 450
Bsp143I GATC 5 cut(s) 270, 700, 1171, 1287, 1396
Bsp19I CCATGG 2 cut(s) 241, 500
BspACI CCGC 5 cut(s) 17, 184, 422, 607, 685
BspANI GGCC 3 cut(s) 231, 1007, 1112
BspCNI CTCAG 6 cut(s) 72, 88, 313, 498, 850, 930
BspHI TCATGA 1 cut(s) 472
BspLI GGNNCC 4 cut(s) 171, 385, 1358, 1459
BspMAI CTGCAG 1 cut(s) 412
BspMI ACCTGC 1 cut(s) 739
BspPI GGATC 3 cut(s) 265, 1179, 1404
BsrI ACTGG 1 cut(s) 661
BssECI CCNNGG 3 cut(s) 241, 500, 1113
BssMI GATC 5 cut(s) 270, 700, 1171, 1287, 1396
BssT1I CCWWGG 3 cut(s) 241, 500, 1113
Bst2UI CCWGG 2 cut(s) 125, 682
Bst4CI ACNGT 4 cut(s) 26, 1189, 1361, 1420
BstC8I GCNNGC 3 cut(s) 433, 671, 1283
BstDEI CTNAG 9 cut(s) 59, 96, 321, 368, 506, 807, 837, 938, 1027
BstDSI CCRYGG 2 cut(s) 241, 500
BstF5I GGATG 3 cut(s) 331, 1084, 1198
BstKTI GATC 5 cut(s) 273, 703, 1174, 1290, 1399
BstMAI GTCTC 4 cut(s) 604, 989, 1076, 1473
BstMBI GATC 5 cut(s) 270, 700, 1171, 1287, 1396
BstMCI CGRYCG 1 cut(s) 1324
BstMWI GCNNNNNNNGC 5 cut(s) 183, 428, 675, 731, 752
BstNI CCWGG 2 cut(s) 125, 682
BstNSI RCATGY 3 cut(s) 599, 1035, 1237
BstPAI GACNNNNGTC 1 cut(s) 34
BstSCI CCNGG 2 cut(s) 123, 680
BstSFI CTRYAG 2 cut(s) 408, 516
BstV1I GCAGC 4 cut(s) 212, 653, 913, 1254
BstV2I GAAGAC 1 cut(s) 323
BstX2I RGATCY 2 cut(s) 270, 1396
BstXI CCANNNNNNTGG 1 cut(s) 153
BstYI RGATCY 2 cut(s) 270, 1396
BsuI GTATCC 2 cut(s) 681, 1140
BsuRI GGCC 3 cut(s) 231, 1007, 1112
BtgI CCRYGG 2 cut(s) 241, 500
BtsCI GGATG 3 cut(s) 331, 1084, 1198
BtsI GCAGTG 1 cut(s) 1260
BtsIMutI CAGTG 5 cut(s) 31, 93, 668, 1185, 1260
BveI ACCTGC 1 cut(s) 739
Cac8I GCNNGC 3 cut(s) 433, 671, 1283
CciI TCATGA 1 cut(s) 472
Cfr13I GGNCC 2 cut(s) 230, 384
Csp6I GTAC 5 cut(s) 81, 128, 648, 833, 1421
CviAII CATG 9 cut(s) 242, 265, 473, 501, 596, 905, 1032, 1108, 1234
CviQI GTAC 5 cut(s) 81, 128, 648, 833, 1421
DdeI CTNAG 9 cut(s) 59, 96, 321, 368, 506, 807, 837, 938, 1027
DpnI GATC 5 cut(s) 272, 702, 1173, 1289, 1398
DpnII GATC 5 cut(s) 270, 700, 1171, 1287, 1396
DraI TTTAAA 1 cut(s) 333
EciI GGCGGA 1 cut(s) 700
Ecl136II GAGCTC 1 cut(s) 448
Eco130I CCWWGG 3 cut(s) 241, 500, 1113
Eco24I GRGCYC 1 cut(s) 450
Eco47I GGWCC 1 cut(s) 384
Eco53kI GAGCTC 1 cut(s) 448
EcoICRI GAGCTC 1 cut(s) 448
EcoO109I RGGNCCY 1 cut(s) 230
EcoRII CCWGG 2 cut(s) 123, 680
EcoT14I CCWWGG 3 cut(s) 241, 500, 1113
EcoT38I GRGCYC 1 cut(s) 450
ErhI CCWWGG 3 cut(s) 241, 500, 1113
FaeI CATG 9 cut(s) 245, 268, 476, 504, 599, 908, 1035, 1111, 1237
FalI AAGNNNNNCTT 8 cut(s) 351, 383, 735, 767, 965, 997, 1010, 1042
FaqI GGGAC 1 cut(s) 397
FatI CATG 9 cut(s) 241, 264, 472, 500, 595, 904, 1031, 1107, 1233
FauI CCCGC 1 cut(s) 614
FblI GTMKAC 1 cut(s) 1324
Fnu4HI GCNGC 4 cut(s) 201, 667, 927, 1243
FokI GGATG 3 cut(s) 338, 1091, 1205
FriOI GRGCYC 1 cut(s) 450
Fsp4HI GCNGC 4 cut(s) 201, 667, 927, 1243
FspBI CTAG 1 cut(s) 1238
GluI GCNGC 4 cut(s) 201, 667, 927, 1243
GsaI CCCAGC 1 cut(s) 177
HaeIII GGCC 3 cut(s) 231, 1007, 1112
Hin1II CATG 9 cut(s) 245, 268, 476, 504, 599, 908, 1035, 1111, 1237
HincII GTYRAC 2 cut(s) 7, 1325
HindII GTYRAC 2 cut(s) 7, 1325
HindIII AAGCTT 2 cut(s) 392, 1055
HinfI GANTC 2 cut(s) 35, 631
HphI GGTGA 3 cut(s) 489, 844, 964
Hpy188I TCNGA 3 cut(s) 582, 1047, 1101
Hpy188III TCNNGA 7 cut(s) 302, 473, 569, 823, 881, 985, 1402
Hpy99I CGWCG 1 cut(s) 824
HpyAV CCTTC 3 cut(s) 63, 702, 991
HpyCH4III ACNGT 4 cut(s) 26, 1189, 1361, 1420
HpyCH4IV ACGT 2 cut(s) 83, 307
HpyCH4V TGCA 4 cut(s) 410, 595, 929, 1152
HpyF10VI GCNNNNNNNGC 5 cut(s) 183, 428, 675, 731, 752
HpyF3I CTNAG 9 cut(s) 59, 96, 321, 368, 506, 807, 837, 938, 1027
HpySE526I ACGT 2 cut(s) 83, 307
Hsp92II CATG 9 cut(s) 245, 268, 476, 504, 599, 908, 1035, 1111, 1237
Kzo9I GATC 5 cut(s) 270, 700, 1171, 1287, 1396
LmnI GCTCC 2 cut(s) 683, 1117
Lsp1109I GCAGC 4 cut(s) 212, 653, 913, 1254
LweI GCATC 2 cut(s) 418, 953
MaeI CTAG 1 cut(s) 1238
MaeII ACGT 2 cut(s) 83, 307
MaeIII GTNAC 2 cut(s) 84, 374
MalI GATC 5 cut(s) 272, 702, 1173, 1289, 1398
MboI GATC 5 cut(s) 270, 700, 1171, 1287, 1396
MboII GAAGA 9 cut(s) 128, 328, 554, 557, 629, 632, 635, 784, 1307
MflI RGATCY 2 cut(s) 270, 1396
MhlI GDGCHC 1 cut(s) 450
MluCI AATT 6 cut(s) 215, 338, 417, 1153, 1224, 1301
MlyI GAGTC 2 cut(s) 44, 625
MnlI CCTC 5 cut(s) 222, 316, 501, 846, 933
MroXI GAANNNNTTC 2 cut(s) 848, 980
MslI CAYNNNNRTG 2 cut(s) 44, 477
MspA1I CMGCKG 1 cut(s) 1177
MspR9I CCNGG 2 cut(s) 125, 682
Mva1269I GAATGC 1 cut(s) 19
MvaI CCWGG 2 cut(s) 125, 682
MwoI GCNNNNNNNGC 5 cut(s) 183, 428, 675, 731, 752
NcoI CCATGG 2 cut(s) 241, 500
NdeII GATC 5 cut(s) 270, 700, 1171, 1287, 1396
NlaIII CATG 9 cut(s) 245, 268, 476, 504, 599, 908, 1035, 1111, 1237
NlaIV GGNNCC 4 cut(s) 171, 385, 1358, 1459
NmuCI GTSAC 1 cut(s) 84
NspI RCATGY 3 cut(s) 599, 1035, 1237
PagI TCATGA 1 cut(s) 472
PctI GAATGC 1 cut(s) 19
PdmI GAANNNNTTC 2 cut(s) 848, 980
PkrI GCNGC 4 cut(s) 202, 668, 928, 1244
PleI GAGTC 2 cut(s) 43, 625
PpsI GAGTC 2 cut(s) 43, 625
PshAI GACNNNNGTC 1 cut(s) 34
PsiI TTATAA 1 cut(s) 291
Psp124BI GAGCTC 1 cut(s) 450
Psp1406I AACGTT 1 cut(s) 307
Psp6I CCWGG 2 cut(s) 123, 680
PspFI CCCAGC 1 cut(s) 173
PspGI CCWGG 2 cut(s) 123, 680
PspN4I GGNNCC 4 cut(s) 171, 385, 1358, 1459
PspPI GGNCC 2 cut(s) 230, 384
PstI CTGCAG 1 cut(s) 412
PsuI RGATCY 2 cut(s) 270, 1396
PvuII CAGCTG 1 cut(s) 1177
RsaI GTAC 5 cut(s) 82, 129, 649, 834, 1422
RsaNI GTAC 5 cut(s) 81, 128, 648, 833, 1421
RseI CAYNNNNRTG 2 cut(s) 44, 477
SacI GAGCTC 1 cut(s) 450
SalI GTCGAC 1 cut(s) 1323
SatI GCNGC 4 cut(s) 201, 667, 927, 1243
Sau3AI GATC 5 cut(s) 270, 700, 1171, 1287, 1396
Sau96I GGNCC 2 cut(s) 230, 384
SchI GAGTC 2 cut(s) 44, 625
ScrFI CCNGG 2 cut(s) 125, 682
SduI GDGCHC 1 cut(s) 450
SfaNI GCATC 2 cut(s) 418, 953
SfcI CTRYAG 2 cut(s) 408, 516
SinI GGWCC 1 cut(s) 384
SmiMI CAYNNNNRTG 2 cut(s) 44, 477
SmlI CTYRAG 2 cut(s) 773, 1472
SmoI CTYRAG 2 cut(s) 773, 1472
Sse9I AATT 6 cut(s) 215, 338, 417, 1153, 1224, 1301
SsiI CCGC 5 cut(s) 17, 184, 422, 607, 685
SspI AATATT 1 cut(s) 1351
SspMI CTAG 1 cut(s) 1238
SstI GAGCTC 1 cut(s) 450
StyD4I CCNGG 2 cut(s) 123, 680
StyI CCWWGG 3 cut(s) 241, 500, 1113
TaaI ACNGT 4 cut(s) 26, 1189, 1361, 1420
TaiI ACGT 2 cut(s) 86, 310
TaqI TCGA 4 cut(s) 444, 822, 912, 1324
TaqII GACCGA 2 cut(s) 1310, 1438
TasI AATT 6 cut(s) 215, 338, 417, 1153, 1224, 1301
TatI WGTACW 1 cut(s) 1420
TscAI CASTG 5 cut(s) 31, 100, 668, 1192, 1260
TseFI GTSAC 1 cut(s) 84
TseI GCWGC 4 cut(s) 200, 666, 926, 1242
Tsp45I GTSAC 1 cut(s) 84
TspDTI ATGAA 3 cut(s) 210, 427, 489
TspGWI ACGGA 1 cut(s) 808
TspRI CASTG 5 cut(s) 31, 100, 668, 1192, 1260
VpaK11BI GGWCC 1 cut(s) 384
XapI RAATTY 1 cut(s) 1301
XceI RCATGY 3 cut(s) 599, 1035, 1237
XmiI GTMKAC 1 cut(s) 1324
XmnI GAANNNNTTC 2 cut(s) 848, 980
XspI CTAG 1 cut(s) 1238
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.