AT3G60470

Plant protein of unknown function

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0000821
Physical Location & Seq
Forward (+)
22344758 .. 22347121
2364 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G60470.1

Sequence Viewer

Length: 1623 bp
ATGAACCGAAGGTTGAATCATGAACCGGACACGGTCCTTGAGTTAAACGAATCGACTCCTGGGATGTGGTGGTTTCCTACAAATCCTGAGCATCGTTGCATCTACCGAGTCCCTGACCGTCTACGTCGTGTAAATCCAGAAGCTTACACGCCTCAATTACTCCTCATTGGACCTCTTCACCATTCTTTAAAATTTCAAGCTCTCAACTCTCGTGGAGATATCACAAACACAAAGTTAATGGGCTACTTAAACATGGAGGAGCATAAGAAGATTTACCTTGCGAGATTCTCAGAAAGGCTTGCAGGTCAAAAAACGATTGATGAACTCAGAAGAAGAATCAAAGAAGAAGAAGATATAATAAGGGCAAGCTACTCTGAGTCAACTGCCTGGATTGAATCTCCAAAGTTCGTGGACATGATCCTACACGACTCTGTTTTCATAATAGAGTTCCTTTTAAGGAGTAAAGATTACACCTTACTTGAGGACCAAGGATCATGCATCAAGAAAACATGGGATCCTATATTTGAGCAGCCTTGTCTTCAAACTACTGTGGATGAAGACCTCATTTTGCTCGAGAATCAACTTCCTTACTTCATCCTCGAGAAACTCTTCGATCCAATCGTTCCAATACTCCGCCCAAACCAAACACTCCGCCAGATAGTCATCACTTACTTCAGATTCCAAGGTAATATCGGAGTTGACTCCAGATTTAGACATTTCACTGATCTATTCCGATGTGTCCGCGTGGAGACACTTAGGCCAAAGATGCCAAAGAATAAATTCAGATATTTAGTTGGTCTGTTCCGATTTGTCCATTTGGGAACACACGGGTCAAGAGAATTAGAAAAGAGTCGTCCTCAAGAACATAAAGCTCTTCGCATGGAGAAGACACATGGGTCAGGAGAACCAACAAAGAGCACATTCAGATATATAGCTGATCTTTTCCGGTGTGTTCCGATGGAGACACTAGGTTCGAAAAGAGAAGAAAAGAGTCGTCTTGAACGACTCAAAACTCATCGCATGGAGGAGACGTCACATGGTCATGGCTCAAAAAAAACAAAAGAGCTACCGATCATCAAACACATGTATAACGCGGCAAAGCTACAAAGTGCAGGAGTGAAGTTCAAGGCCGTGACAGATGAGTTCTCGATAGATGTGACATTTGAGAATGGTTGTTTGAAGATACCTTGTTTGTGGGTTCCTGATGATGCAGAGATCACACTGAGAAATATAATGGCGCTTGAGCAATGCCATTACCCTTTCAAAGCATACGTCTGTAACTTCGTCAGTTTCTTGGATTTCCTCATCGACACTGATAAAGACGTGGACTTACTCATGGAGAACGGGATACTAAACAACTGGGGGGAGAAGAGTTCTGTGGCGGTGGCGGAGATGGTGAACACGCTATTTTCGGGGGTGGTGGAGAGTCGTTCTTACTATGCTGGTATAGCGTCTAGAGTCAACGCCTATTATGAAAATCCAGTCAACAGGTCACGCACCATCCTCGGACGCCAGTATTTCGGTAATCTATGGAGAGGTACCGCTACTGTCGCCGCTGCGCTTCTTTTGGTGATGACTCTTATCCAGACGGTGGCTTCTATTCTTCAGGTTATGCAAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

540

Amino Acids

62.78

Weight (kDa)

8.76

Isoelectric Point (pI)

43.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 34 - 525 4.9e-104 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 895
AatII GACGTC 1 cut(s) 1034
Acc36I ACCTGC 1 cut(s) 293
Acc65I GGTACC 1 cut(s) 1538
AccB1I GGYRCC 1 cut(s) 1538
AccB7I CCANNNNNTGG 1 cut(s) 1591
AccI GTMKAC 1 cut(s) 121
AccII CGCG 2 cut(s) 744, 1094
AciI CCGC 8 cut(s) 634, 652, 742, 1094, 1382, 1388, 1542, 1554
AclWI GGATC 5 cut(s) 412, 499, 509, 522, 608
AcsI RAATTY 2 cut(s) 191, 779
AcuI CTGAAG 2 cut(s) 658, 1589
AcyI GRCGYC 2 cut(s) 1031, 1510
AfaI GTAC 1 cut(s) 1540
AfiI CCNNNNNNNGG 1 cut(s) 1591
AflIII ACRYGT 1 cut(s) 1083
AgsI TTSAA 8 cut(s) 16, 197, 395, 542, 1001, 1126, 1180, 1264
AjiI CACGTC 1 cut(s) 1324
AjnI CCWGG 2 cut(s) 58, 386
AluBI AGCT 7 cut(s) 143, 200, 369, 872, 935, 1066, 1102
AluI AGCT 7 cut(s) 143, 200, 369, 872, 935, 1066, 1102
Alw21I GWGCWC 1 cut(s) 920
Alw26I GTCTC 3 cut(s) 743, 956, 1022
AlwI GGATC 5 cut(s) 412, 499, 509, 522, 608
Ama87I CYCGRG 2 cut(s) 572, 599
AoxI GGCC 2 cut(s) 758, 1128
ApeKI GCWGC 2 cut(s) 529, 1556
ApoI RAATTY 2 cut(s) 191, 779
ArsI GACNNNNNNTTYG 4 cut(s) 955, 987, 1257, 1289
Asp700I GAANNNNTTC 2 cut(s) 608, 779
Asp718I GGTACC 1 cut(s) 1538
AspLEI GCGC 2 cut(s) 1240, 1561
AspS9I GGNCC 3 cut(s) 34, 170, 484
AsuHPI GGTGA 3 cut(s) 170, 1408, 1582
AsuII TTCGAA 1 cut(s) 974
AvaI CYCGRG 2 cut(s) 572, 599
AvaII GGWCC 3 cut(s) 34, 170, 484
BamHI GGATCC 1 cut(s) 514
BanI GGYRCC 1 cut(s) 1538
BauI CACGAG 1 cut(s) 210
BbsI GAAGAC 3 cut(s) 530, 564, 893
Bbv12I GWGCWC 1 cut(s) 920
BbvI GCAGC 2 cut(s) 541, 1543
BccI CCATC 3 cut(s) 952, 1387, 1508
BceAI ACGGC 1 cut(s) 1115
BcgI CGANNNNNNTGC 2 cut(s) 1486, 1520
BciT130I CCWGG 2 cut(s) 60, 388
BciVI GTATCC 1 cut(s) 1341
BcoDI GTCTC 3 cut(s) 743, 956, 1022
BfaI CTAG 2 cut(s) 968, 1455
BfoI RGCGCY 1 cut(s) 1241
BfuAI ACCTGC 1 cut(s) 293
BfuI GTATCC 1 cut(s) 1341
BisI GCNGC 4 cut(s) 530, 1095, 1554, 1557
BlsI GCNGC 4 cut(s) 531, 1096, 1555, 1558
Bme1390I CCNGG 2 cut(s) 60, 388
Bme18I GGWCC 3 cut(s) 34, 170, 484
BmeT110I CYCGRG 2 cut(s) 572, 599
BmgBI CACGTC 1 cut(s) 1324
BmgT120I GGNCC 3 cut(s) 34, 170, 484
BmiI GGNNCC 3 cut(s) 516, 1200, 1540
BmrFI CCNGG 2 cut(s) 60, 388
BmrI ACTGGG 1 cut(s) 1369
BmsI GCATC 5 cut(s) 100, 108, 507, 756, 1198
BmuI ACTGGG 1 cut(s) 1369
BpiI GAAGAC 3 cut(s) 530, 564, 893
BplI GAGNNNNNCTC 2 cut(s) 841, 873
BpmI CTGGAG 1 cut(s) 688
Bpu10I CCTNAGC 1 cut(s) 87
Bpu14I TTCGAA 1 cut(s) 974
BpuEI CTTGAG 4 cut(s) 59, 500, 843, 1262
BsaBI GATNNNNATC 1 cut(s) 662
BsaHI GRCGYC 2 cut(s) 1031, 1510
BsaJI CCNNGG 4 cut(s) 59, 487, 682, 1504
BsaWI WCCGGW 2 cut(s) 25, 945
BsaXI ACNNNNNCTCC 2 cut(s) 1016, 1046
Bsc4I CCNNNNNNNGG 1 cut(s) 1591
Bse1I ACTGG 3 cut(s) 1364, 1481, 1513
Bse3DI GCAATG 1 cut(s) 1253
Bse8I GATNNNNATC 1 cut(s) 662
BseBI CCWGG 2 cut(s) 60, 388
BseDI CCNNGG 4 cut(s) 59, 487, 682, 1504
BseGI GGATG 4 cut(s) 69, 559, 594, 1500
BseJI GATNNNNATC 1 cut(s) 662
BseLI CCNNNNNNNGG 1 cut(s) 1591
BseMI GCAATG 1 cut(s) 1253
BseMII CTCAG 5 cut(s) 78, 303, 340, 366, 1214
BseNI ACTGG 3 cut(s) 1364, 1481, 1513
BseRI GAGGAG 3 cut(s) 152, 272, 1040
BseXI GCAGC 2 cut(s) 541, 1543
BsgI GTGCAG 1 cut(s) 1131
Bsh1236I CGCG 2 cut(s) 744, 1094
BshFI GGCC 2 cut(s) 760, 1130
BshNI GGYRCC 1 cut(s) 1538
BsiHKAI GWGCWC 1 cut(s) 920
BsiHKCI CYCGRG 2 cut(s) 572, 599
BsiSI CCGG 2 cut(s) 26, 946
BslFI GGGAC 1 cut(s) 95
BslI CCNNNNNNNGG 1 cut(s) 1591
BsmAI GTCTC 3 cut(s) 743, 956, 1022
BsmBI CGTCTC 1 cut(s) 1022
BsmFI GGGAC 1 cut(s) 95
BsnI GGCC 2 cut(s) 760, 1130
BsoBI CYCGRG 2 cut(s) 572, 599
Bsp119I TTCGAA 1 cut(s) 974
Bsp1286I GDGCHC 1 cut(s) 920
Bsp143I GATC 8 cut(s) 417, 491, 514, 613, 724, 937, 1071, 1215
BspACI CCGC 8 cut(s) 634, 652, 742, 1094, 1382, 1388, 1542, 1554
BspANI GGCC 2 cut(s) 760, 1130
BspCNI CTCAG 5 cut(s) 79, 302, 339, 367, 1215
BspFNI CGCG 2 cut(s) 744, 1094
BspHI TCATGA 1 cut(s) 19
BspLI GGNNCC 3 cut(s) 516, 1200, 1540
BspMI ACCTGC 1 cut(s) 293
BspPI GGATC 5 cut(s) 412, 499, 509, 522, 608
BspQI GCTCTTC 1 cut(s) 879
BspT104I TTCGAA 1 cut(s) 974
BspT107I GGYRCC 1 cut(s) 1538
BsrDI GCAATG 1 cut(s) 1253
BsrI ACTGG 3 cut(s) 1364, 1481, 1513
BssECI CCNNGG 4 cut(s) 59, 487, 682, 1504
BssMI GATC 8 cut(s) 417, 491, 514, 613, 724, 937, 1071, 1215
BssNI GRCGYC 2 cut(s) 1031, 1510
BssSI CACGAG 1 cut(s) 210
BssT1I CCWWGG 2 cut(s) 487, 682
Bst2BI CACGAG 1 cut(s) 210
Bst2UI CCWGG 2 cut(s) 60, 388
Bst4CI ACNGT 5 cut(s) 34, 119, 550, 1549, 1591
Bst6I CTCTTC 4 cut(s) 180, 614, 879, 1364
BstACI GRCGYC 2 cut(s) 1031, 1510
BstBI TTCGAA 1 cut(s) 974
BstC8I GCNNGC 2 cut(s) 300, 367
BstDEI CTNAG 6 cut(s) 87, 289, 326, 375, 755, 1223
BstF5I GGATG 4 cut(s) 69, 559, 594, 1500
BstFNI CGCG 2 cut(s) 744, 1094
BstH2I RGCGCY 1 cut(s) 1241
BstHHI GCGC 2 cut(s) 1240, 1561
BstKTI GATC 8 cut(s) 420, 494, 517, 616, 727, 940, 1074, 1218
BstMAI GTCTC 3 cut(s) 743, 956, 1022
BstMBI GATC 8 cut(s) 417, 491, 514, 613, 724, 937, 1071, 1215
BstMWI GCNNNNNNNGC 3 cut(s) 766, 1448, 1550
BstNI CCWGG 2 cut(s) 60, 388
BstNSI RCATGY 1 cut(s) 1087
BstSCI CCNGG 2 cut(s) 58, 386
BstUI CGCG 2 cut(s) 744, 1094
BstV1I GCAGC 2 cut(s) 541, 1543
BstV2I GAAGAC 3 cut(s) 530, 564, 893
BstX2I RGATCY 1 cut(s) 514
BstYI RGATCY 1 cut(s) 514
BsuI GTATCC 1 cut(s) 1341
BsuRI GGCC 2 cut(s) 760, 1130
BtgZI GCGATG 1 cut(s) 1001
BtrI CACGTC 1 cut(s) 1324
BtsCI GGATG 4 cut(s) 69, 559, 594, 1500
BtsIMutI CAGTG 3 cut(s) 720, 1220, 1311
BveI ACCTGC 1 cut(s) 293
Cac8I GCNNGC 2 cut(s) 300, 367
CciI TCATGA 1 cut(s) 19
CfoI GCGC 2 cut(s) 1240, 1561
Cfr13I GGNCC 3 cut(s) 34, 170, 484
CseI GACGC 2 cut(s) 1440, 1518
Csp6I GTAC 1 cut(s) 1539
CspCI CAANNNNNGTGG 4 cut(s) 193, 228, 390, 425
CviQI GTAC 1 cut(s) 1539
DdeI CTNAG 6 cut(s) 87, 289, 326, 375, 755, 1223
DpnI GATC 8 cut(s) 419, 493, 516, 615, 726, 939, 1073, 1217
DpnII GATC 8 cut(s) 417, 491, 514, 613, 724, 937, 1071, 1215
DraI TTTAAA 1 cut(s) 189
DrdI GACNNNNNNGTC 1 cut(s) 895
DseDI GACNNNNNNGTC 1 cut(s) 895
Eam1104I CTCTTC 4 cut(s) 180, 614, 879, 1364
EarI CTCTTC 4 cut(s) 180, 614, 879, 1364
EciI GGCGGA 3 cut(s) 623, 641, 1403
Eco130I CCWWGG 2 cut(s) 487, 682
Eco32I GATATC 1 cut(s) 220
Eco47I GGWCC 3 cut(s) 34, 170, 484
Eco57I CTGAAG 2 cut(s) 658, 1589
Eco88I CYCGRG 2 cut(s) 572, 599
EcoRII CCWGG 2 cut(s) 58, 386
EcoRV GATATC 1 cut(s) 220
EcoT14I CCWWGG 2 cut(s) 487, 682
EcoT22I ATGCAT 1 cut(s) 500
ErhI CCWWGG 2 cut(s) 487, 682
Esp3I CGTCTC 1 cut(s) 1022
FaqI GGGAC 1 cut(s) 95
FblI GTMKAC 1 cut(s) 121
Fnu4HI GCNGC 4 cut(s) 530, 1095, 1554, 1557
FokI GGATG 4 cut(s) 76, 566, 581, 1487
Fsp4HI GCNGC 4 cut(s) 530, 1095, 1554, 1557
FspBI CTAG 2 cut(s) 968, 1455
GlaI GCGC 2 cut(s) 1239, 1560
GluI GCNGC 4 cut(s) 530, 1095, 1554, 1557
GsuI CTGGAG 1 cut(s) 688
HaeII RGCGCY 1 cut(s) 1241
HaeIII GGCC 2 cut(s) 760, 1130
HapII CCGG 2 cut(s) 26, 946
HgaI GACGC 2 cut(s) 1440, 1518
HhaI GCGC 2 cut(s) 1240, 1561
Hin1I GRCGYC 2 cut(s) 1031, 1510
Hin6I GCGC 2 cut(s) 1238, 1559
HinP1I GCGC 2 cut(s) 1238, 1559
HincII GTYRAC 4 cut(s) 381, 700, 1462, 1486
HindII GTYRAC 4 cut(s) 381, 700, 1462, 1486
HindIII AAGCTT 1 cut(s) 141
HpaII CCGG 2 cut(s) 26, 946
HphI GGTGA 3 cut(s) 170, 1408, 1582
Hpy166II GTNNAC 8 cut(s) 122, 381, 412, 700, 1327, 1399, 1462, 1486
Hpy8I GTNNAC 8 cut(s) 122, 381, 412, 700, 1327, 1399, 1462, 1486
Hpy99I CGWCG 1 cut(s) 129
HpyAV CCTTC 1 cut(s) 3
HpyCH4III ACNGT 5 cut(s) 34, 119, 550, 1549, 1591
HpyCH4IV ACGT 4 cut(s) 124, 1031, 1272, 1323
HpyCH4V TGCA 6 cut(s) 99, 302, 498, 1112, 1211, 1615
HpyF10VI GCNNNNNNNGC 3 cut(s) 766, 1448, 1550
HpyF3I CTNAG 6 cut(s) 87, 289, 326, 375, 755, 1223
HpySE526I ACGT 4 cut(s) 124, 1031, 1272, 1323
Hsp92I GRCGYC 2 cut(s) 1031, 1510
HspAI GCGC 2 cut(s) 1238, 1559
KpnI GGTACC 1 cut(s) 1542
Kzo9I GATC 8 cut(s) 417, 491, 514, 613, 724, 937, 1071, 1215
LguI GCTCTTC 1 cut(s) 879
LmnI GCTCC 1 cut(s) 259
Lsp1109I GCAGC 2 cut(s) 541, 1543
LweI GCATC 5 cut(s) 100, 108, 507, 756, 1198
MaeI CTAG 2 cut(s) 968, 1455
MaeII ACGT 4 cut(s) 124, 1031, 1272, 1323
MaeIII GTNAC 5 cut(s) 1032, 1132, 1156, 1277, 1491
MalI GATC 8 cut(s) 419, 493, 516, 615, 726, 939, 1073, 1217
MboI GATC 8 cut(s) 417, 491, 514, 613, 724, 937, 1071, 1215
MflI RGATCY 1 cut(s) 514
MhlI GDGCHC 1 cut(s) 920
MluCI AATT 4 cut(s) 155, 191, 779, 839
Mph1103I ATGCAT 1 cut(s) 500
MroXI GAANNNNTTC 2 cut(s) 608, 779
MseI TTAA 5 cut(s) 44, 188, 236, 248, 455
MslI CAYNNNNRTG 1 cut(s) 1041
MspA1I CMGCKG 1 cut(s) 1556
MspI CCGG 2 cut(s) 26, 946
MspR9I CCNGG 2 cut(s) 60, 388
MvaI CCWGG 2 cut(s) 60, 388
MvnI CGCG 2 cut(s) 744, 1094
MwoI GCNNNNNNNGC 3 cut(s) 766, 1448, 1550
NdeII GATC 8 cut(s) 417, 491, 514, 613, 724, 937, 1071, 1215
NlaIV GGNNCC 3 cut(s) 516, 1200, 1540
NmuCI GTSAC 4 cut(s) 1032, 1132, 1156, 1491
NsiI ATGCAT 1 cut(s) 500
NspI RCATGY 1 cut(s) 1087
NspV TTCGAA 1 cut(s) 974
PaeR7I CTCGAG 2 cut(s) 572, 599
PagI TCATGA 1 cut(s) 19
PciI ACATGT 1 cut(s) 1083
PciSI GCTCTTC 1 cut(s) 879
PcsI WCGNNNNNNNCGW 2 cut(s) 618, 1000
PdmI GAANNNNTTC 2 cut(s) 608, 779
PfeI GAWTC 7 cut(s) 16, 50, 285, 336, 395, 577, 678
PflFI GACNNNGTC 1 cut(s) 32
PflMI CCANNNNNTGG 1 cut(s) 1591
PkrI GCNGC 4 cut(s) 531, 1096, 1555, 1558
PscI ACATGT 1 cut(s) 1083
Psp6I CCWGG 2 cut(s) 58, 386
PspGI CCWGG 2 cut(s) 58, 386
PspN4I GGNNCC 3 cut(s) 516, 1200, 1540
PspPI GGNCC 3 cut(s) 34, 170, 484
PsuI RGATCY 1 cut(s) 514
PsyI GACNNNGTC 1 cut(s) 32
RsaI GTAC 1 cut(s) 1540
RsaNI GTAC 1 cut(s) 1539
RseI CAYNNNNRTG 1 cut(s) 1041
SapI GCTCTTC 1 cut(s) 879
SaqAI TTAA 5 cut(s) 44, 188, 236, 248, 455
SatI GCNGC 4 cut(s) 530, 1095, 1554, 1557
Sau3AI GATC 8 cut(s) 417, 491, 514, 613, 724, 937, 1071, 1215
Sau96I GGNCC 3 cut(s) 34, 170, 484
ScrFI CCNGG 2 cut(s) 60, 388
SduI GDGCHC 1 cut(s) 920
SfaNI GCATC 5 cut(s) 100, 108, 507, 756, 1198
Sfr274I CTCGAG 2 cut(s) 572, 599
SfuI TTCGAA 1 cut(s) 974
SinI GGWCC 3 cut(s) 34, 170, 484
SlaI CTCGAG 2 cut(s) 572, 599
SmiMI CAYNNNNRTG 1 cut(s) 1041
SmlI CTYRAG 6 cut(s) 38, 479, 572, 599, 858, 1241
SmoI CTYRAG 6 cut(s) 38, 479, 572, 599, 858, 1241
Sse9I AATT 4 cut(s) 155, 191, 779, 839
SsiI CCGC 8 cut(s) 634, 652, 742, 1094, 1382, 1388, 1542, 1554
SspMI CTAG 2 cut(s) 968, 1455
StyD4I CCNGG 2 cut(s) 58, 386
StyI CCWWGG 2 cut(s) 487, 682
TaaI ACNGT 5 cut(s) 34, 119, 550, 1549, 1591
TaiI ACGT 4 cut(s) 127, 1034, 1275, 1326
TaqI TCGA 7 cut(s) 53, 573, 600, 612, 974, 1148, 1308
TasI AATT 4 cut(s) 155, 191, 779, 839
TauI GCSGC 2 cut(s) 1097, 1556
TfiI GAWTC 7 cut(s) 16, 50, 285, 336, 395, 577, 678
Tru1I TTAA 5 cut(s) 44, 188, 236, 248, 455
Tru9I TTAA 5 cut(s) 44, 188, 236, 248, 455
TscAI CASTG 3 cut(s) 727, 1227, 1318
TseFI GTSAC 4 cut(s) 1032, 1132, 1156, 1491
TseI GCWGC 2 cut(s) 529, 1556
Tsp45I GTSAC 4 cut(s) 1032, 1132, 1156, 1491
TspDTI ATGAA 7 cut(s) 17, 36, 336, 427, 570, 583, 1488
TspRI CASTG 3 cut(s) 727, 1227, 1318
Tth111I GACNNNGTC 1 cut(s) 32
Van91I CCANNNNNTGG 1 cut(s) 1591
VpaK11BI GGWCC 3 cut(s) 34, 170, 484
XapI RAATTY 2 cut(s) 191, 779
XbaI TCTAGA 1 cut(s) 1454
XceI RCATGY 1 cut(s) 1087
XhoI CTCGAG 2 cut(s) 572, 599
XmiI GTMKAC 1 cut(s) 121
XmnI GAANNNNTTC 2 cut(s) 608, 779
XspI CTAG 2 cut(s) 968, 1455
ZraI GACGTC 1 cut(s) 1032
Zsp2I ATGCAT 1 cut(s) 500
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.