FvH4_7g09691

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0000821
Physical Location & Seq
Forward (+)
9311410 .. 9313104
1695 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g09691.t1

Sequence Viewer

Length: 1509 bp
ATGTCGAAAATACAGTTTTTGAGTTTTGCCGTGTTGCCGTGTCAGTATGTATCGTGTCGCCATATCCGTGTCCATGTCCGTGTCCGTGCGTGTCCGTGCAACATAGATTATGAGTCCAAGTCGACTTCACAGAAAACTGGGAGTGGAACTGTACTACTGGCGGACGATCCTGACAAGTGGTGTATCTATAGGGTTCCCAGCAAACTGCGCAATGTGAATAAAGAAGCATACACCCCCCAACTAGTTTCAATTGGCCTTTTCCACCATGGCAAAGCAGAACTGCAAGATATGCAGAACCATAAGAAAAAATATATGGATTACTTTTTTAAGCGCCCTGATTTGAGAGATATGGATGCACTAAGAAGCTTTATTGAGGATCGTAAAAACAAGATTCTTCATTGTTATGCAGGGACGATTGAGCTTGGTGACACTGACCTTGTTGAGATGATTTTAGTTGATGCATGCTTCATTATTGAGCTCTTTCTGATGAAGTACGAGCGAGAAAATGGTGGCGAGTGCACGAAGGATTACATATTAAGTTCACCTTGGGTGCGGAAAGCTGCAGAGCAGGATCTGATTCTGTTTGAAAATCAGCTTCCTTATTTTCTTCTCCAAGAACTCTATGACTTTGCATTTGGAGCGTCTTCCATTTCCCATCCTGTAAAAGAAATACAGTCACATAAATCCAGCACCAATAACAAGCGAAACTGCTTCCAGAAGTGCTTCCCTTGCTACTGTGGGATCCATCCCAATGATCATCATGTTATGAAGATGGATCATGATGAGTCCGAGCCTGAACTATACCATGACAAATTTCTTGAGCTGGCCTGTAATTTCTTCAACAACTACAGTAAAGGAAAATCAGTTAAGGTAAAACCGAAACATTTCACTGATTTGATAAGACATTTTATGTGTCCAGAAGAAAAAATGTCGTGGAAGGAAAATACTACCAATAGTATGTATCGTATGAGAAAGCTGACCGCGGCAGGGGTGAACTTTATGCCATTTCCTAAAGACGGACGCTTTGTGGTGAAAGAAGGTAACATCTTATCATCATTAACATGTTTCAAAACGATGGACTTGAAGCTATCACATTTCTGTGTAAAGGATGAGACGGAATGTATCATCCGCAATGTCATGGCAGCTGAGCAGTTTCTGTATCCAGACGATGCTTATGTTTGCAATTACTTCTTGCTGATGGATAAACTTGTGGACACTGTGGAAGATGTAGAATTTCTGATTCGGCAAAAGGTGATCGTTAACATGCTAGGCAGCGAAGAAGCAGTGGTGAACCTGGTGAATCACCTTTGTGATCATATTCTCGAAGAAAGATCGTGCTATGTTGATATCTGTAAAAAACTGAACAAGCACTATGAGAATTCTTGGAACCGTCATGTGACAACCTTGAGACGAGTTTACTTCAAGGATCTCTGGACAAGCAGCTCAACATTCTTCGGAATTGTTGTTCTTGTTTTCTCTATCATTGGATCCATCAAGTCATTCAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

503

Amino Acids

58.65

Weight (kDa)

7.76

Isoelectric Point (pI)

48.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 62 - 492 4.2e-94 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 209
AccI GTMKAC 1 cut(s) 122
AccII CGCG 1 cut(s) 983
AciI CCGC 5 cut(s) 161, 553, 981, 983, 1129
AclWI GGATC 9 cut(s) 161, 384, 579, 736, 749, 783, 1434, 1482, 1495
AcsI RAATTY 3 cut(s) 812, 1232, 1378
AfaI GTAC 2 cut(s) 153, 494
AfiI CCNNNNNNNGG 2 cut(s) 987, 1016
AflIII ACRYGT 1 cut(s) 1061
AgsI TTSAA 7 cut(s) 249, 587, 841, 1069, 1084, 1423, 1504
AhlI ACTAGT 1 cut(s) 241
AjnI CCWGG 1 cut(s) 1293
AleI CACNNNNGTG 1 cut(s) 1308
AloI GAACNNNNNNTCC 2 cut(s) 1449, 1481
Alw21I GWGCWC 2 cut(s) 480, 521
Alw26I GTCTC 2 cut(s) 1106, 1402
Alw44I GTGCAC 1 cut(s) 517
AlwI GGATC 9 cut(s) 161, 384, 579, 736, 749, 783, 1434, 1482, 1495
AlwNI CAGNNNCTG 2 cut(s) 574, 1156
AoxI GGCC 2 cut(s) 253, 825
ApaLI GTGCAC 1 cut(s) 517
ApeKI GCWGC 4 cut(s) 560, 1142, 1272, 1440
ApoI RAATTY 3 cut(s) 812, 1232, 1378
ArsI GACNNNNNNTTYG 4 cut(s) 617, 649, 1007, 1039
Asp700I GAANNNNTTC 2 cut(s) 722, 884
AspLEI GCGC 2 cut(s) 210, 333
AsuHPI GGTGA 8 cut(s) 437, 534, 1003, 1042, 1264, 1295, 1300, 1309
BaeGI GKGCMC 1 cut(s) 521
BamHI GGATCC 2 cut(s) 741, 1487
BanII GRGCYC 1 cut(s) 480
BbsI GAAGAC 1 cut(s) 636
Bbv12I GWGCWC 2 cut(s) 480, 521
BbvI GCAGC 4 cut(s) 547, 1154, 1284, 1452
BccI CCATC 6 cut(s) 663, 753, 766, 1069, 1192, 1499
BceAI ACGGC 2 cut(s) 14, 22
BciT130I CCWGG 1 cut(s) 1295
BciVI GTATCC 1 cut(s) 1170
BclI TGATCA 2 cut(s) 754, 1312
BcoDI GTCTC 2 cut(s) 1106, 1402
BcuI ACTAGT 1 cut(s) 241
BfaI CTAG 2 cut(s) 242, 1268
BfmI CTRYAG 3 cut(s) 187, 561, 847
BfoI RGCGCY 1 cut(s) 334
BfuI GTATCC 1 cut(s) 1170
BisI GCNGC 5 cut(s) 561, 984, 1143, 1273, 1441
BlpI GCTNAGC 1 cut(s) 1146
BlsI GCNGC 5 cut(s) 562, 985, 1144, 1274, 1442
Bme1390I CCNGG 1 cut(s) 1295
BmiI GGNNCC 4 cut(s) 195, 743, 1388, 1489
BmrFI CCNGG 1 cut(s) 1295
BmrI ACTGGG 1 cut(s) 147
BmsI GCATC 3 cut(s) 343, 448, 1159
BmuI ACTGGG 1 cut(s) 147
BpiI GAAGAC 1 cut(s) 636
Bpu1102I GCTNAGC 1 cut(s) 1146
BpuEI CTTGAG 2 cut(s) 839, 1426
BsaJI CCNNGG 3 cut(s) 265, 545, 981
Bsc4I CCNNNNNNNGG 2 cut(s) 987, 1016
Bse1I ACTGG 2 cut(s) 142, 162
Bse3DI GCAATG 2 cut(s) 217, 1138
BseBI CCWGG 1 cut(s) 1295
BseDI CCNNGG 3 cut(s) 265, 545, 981
BseGI GGATG 5 cut(s) 358, 655, 745, 1114, 1125
BseLI CCNNNNNNNGG 2 cut(s) 987, 1016
BseMI GCAATG 2 cut(s) 217, 1138
BseMII CTCAG 1 cut(s) 1137
BseNI ACTGG 2 cut(s) 142, 162
BseSI GKGCMC 1 cut(s) 521
BseXI GCAGC 4 cut(s) 547, 1154, 1284, 1452
BseYI CCCAGC 1 cut(s) 197
Bsh1236I CGCG 1 cut(s) 983
BshFI GGCC 2 cut(s) 255, 827
BsiHKAI GWGCWC 2 cut(s) 480, 521
BslFI GGGAC 1 cut(s) 424
BslI CCNNNNNNNGG 2 cut(s) 987, 1016
BsmAI GTCTC 2 cut(s) 1106, 1402
BsmBI CGTCTC 2 cut(s) 1106, 1402
BsmFI GGGAC 1 cut(s) 424
BsnI GGCC 2 cut(s) 255, 827
Bsp1286I GDGCHC 2 cut(s) 480, 521
Bsp1720I GCTNAGC 1 cut(s) 1146
Bsp19I CCATGG 1 cut(s) 265
BspACI CCGC 5 cut(s) 161, 553, 981, 983, 1129
BspANI GGCC 2 cut(s) 255, 827
BspCNI CTCAG 1 cut(s) 1138
BspFNI CGCG 1 cut(s) 983
BspHI TCATGA 1 cut(s) 778
BspLI GGNNCC 4 cut(s) 195, 743, 1388, 1489
BspMAI CTGCAG 1 cut(s) 565
BspPI GGATC 9 cut(s) 161, 384, 579, 736, 749, 783, 1434, 1482, 1495
BsrDI GCAATG 2 cut(s) 217, 1138
BsrI ACTGG 2 cut(s) 142, 162
BssECI CCNNGG 3 cut(s) 265, 545, 981
BssT1I CCWWGG 2 cut(s) 265, 545
Bst2UI CCWGG 1 cut(s) 1295
Bst4CI ACNGT 7 cut(s) 15, 151, 675, 737, 851, 1219, 1391
BstAPI GCANNNNNTGC 1 cut(s) 289
BstC8I GCNNGC 2 cut(s) 463, 825
BstDEI CTNAG 2 cut(s) 359, 1146
BstDSI CCRYGG 2 cut(s) 265, 981
BstF5I GGATG 5 cut(s) 358, 655, 745, 1114, 1125
BstFNI CGCG 1 cut(s) 983
BstH2I RGCGCY 1 cut(s) 334
BstHHI GCGC 2 cut(s) 210, 333
BstMAI GTCTC 2 cut(s) 1106, 1402
BstMWI GCNNNNNNNGC 4 cut(s) 207, 289, 638, 729
BstNI CCWGG 1 cut(s) 1295
BstNSI RCATGY 3 cut(s) 465, 1065, 1267
BstSCI CCNGG 1 cut(s) 1293
BstSFI CTRYAG 3 cut(s) 187, 561, 847
BstSLI GKGCMC 1 cut(s) 521
BstUI CGCG 1 cut(s) 983
BstV1I GCAGC 4 cut(s) 547, 1154, 1284, 1452
BstV2I GAAGAC 1 cut(s) 636
BstX2I RGATCY 4 cut(s) 571, 741, 1426, 1487
BstYI RGATCY 4 cut(s) 571, 741, 1426, 1487
BsuI GTATCC 1 cut(s) 1170
BsuRI GGCC 2 cut(s) 255, 827
BtgI CCRYGG 2 cut(s) 265, 981
BtsCI GGATG 5 cut(s) 358, 655, 745, 1114, 1125
BtsI GCAGTG 1 cut(s) 1290
BtsIMutI CAGTG 4 cut(s) 429, 888, 1215, 1290
Cac8I GCNNGC 2 cut(s) 463, 825
CaiI CAGNNNCTG 2 cut(s) 574, 1156
CciI TCATGA 1 cut(s) 778
CfoI GCGC 2 cut(s) 210, 333
Cfr42I CCGCGG 1 cut(s) 984
CseI GACGC 2 cut(s) 630, 1029
CsiI ACCWGGT 1 cut(s) 1293
Csp6I GTAC 2 cut(s) 152, 493
CviQI GTAC 2 cut(s) 152, 493
DdeI CTNAG 2 cut(s) 359, 1146
EciI GGCGGA 1 cut(s) 176
Ecl136II GAGCTC 1 cut(s) 478
Eco130I CCWWGG 2 cut(s) 265, 545
Eco24I GRGCYC 1 cut(s) 480
Eco32I GATATC 1 cut(s) 1348
Eco53kI GAGCTC 1 cut(s) 478
EcoICRI GAGCTC 1 cut(s) 478
EcoRI GAATTC 1 cut(s) 1378
EcoRII CCWGG 1 cut(s) 1293
EcoRV GATATC 1 cut(s) 1348
EcoT14I CCWWGG 2 cut(s) 265, 545
EcoT22I ATGCAT 1 cut(s) 463
EcoT38I GRGCYC 1 cut(s) 480
ErhI CCWWGG 2 cut(s) 265, 545
Esp3I CGTCTC 2 cut(s) 1106, 1402
FalI AAGNNNNNCTT 2 cut(s) 529, 561
FaqI GGGAC 1 cut(s) 424
FbaI TGATCA 2 cut(s) 754, 1312
FblI GTMKAC 1 cut(s) 122
Fnu4HI GCNGC 5 cut(s) 561, 984, 1143, 1273, 1441
FokI GGATG 5 cut(s) 365, 642, 732, 1112, 1121
FriOI GRGCYC 1 cut(s) 480
Fsp4HI GCNGC 5 cut(s) 561, 984, 1143, 1273, 1441
FspBI CTAG 2 cut(s) 242, 1268
FspI TGCGCA 1 cut(s) 209
GlaI GCGC 2 cut(s) 209, 332
GluI GCNGC 5 cut(s) 561, 984, 1143, 1273, 1441
GsaI CCCAGC 1 cut(s) 201
HaeII RGCGCY 1 cut(s) 334
HaeIII GGCC 2 cut(s) 255, 827
HgaI GACGC 2 cut(s) 630, 1029
HhaI GCGC 2 cut(s) 210, 333
Hin6I GCGC 2 cut(s) 208, 331
HinP1I GCGC 2 cut(s) 208, 331
HincII GTYRAC 2 cut(s) 123, 1261
HindII GTYRAC 2 cut(s) 123, 1261
HindIII AAGCTT 1 cut(s) 364
HinfI GANTC 6 cut(s) 113, 391, 577, 785, 1240, 1300
HpaI GTTAAC 1 cut(s) 1261
HphI GGTGA 8 cut(s) 437, 534, 1003, 1042, 1264, 1295, 1300, 1309
Hpy166II GTNNAC 8 cut(s) 123, 519, 542, 994, 1213, 1261, 1291, 1417
Hpy188I TCNGA 5 cut(s) 486, 576, 790, 1239, 1457
Hpy188III TCNNGA 8 cut(s) 170, 715, 779, 818, 917, 1163, 1322, 1432
Hpy8I GTNNAC 8 cut(s) 123, 519, 542, 994, 1213, 1261, 1291, 1417
HpyAV CCTTC 3 cut(s) 517, 931, 1031
HpyCH4III ACNGT 7 cut(s) 15, 151, 675, 737, 851, 1219, 1391
HpyF10VI GCNNNNNNNGC 4 cut(s) 207, 289, 638, 729
HpyF3I CTNAG 2 cut(s) 359, 1146
HspAI GCGC 2 cut(s) 208, 331
Ksp22I TGATCA 2 cut(s) 754, 1312
KspAI GTTAAC 1 cut(s) 1261
KspI CCGCGG 1 cut(s) 984
LmnI GCTCC 1 cut(s) 638
Lsp1109I GCAGC 4 cut(s) 547, 1154, 1284, 1452
LweI GCATC 3 cut(s) 343, 448, 1159
MabI ACCWGGT 1 cut(s) 1293
MaeI CTAG 2 cut(s) 242, 1268
MaeIII GTNAC 4 cut(s) 425, 675, 1040, 1396
MfeI CAATTG 1 cut(s) 249
MflI RGATCY 4 cut(s) 571, 741, 1426, 1487
MhlI GDGCHC 2 cut(s) 480, 521
MluCI AATT 7 cut(s) 249, 812, 832, 1183, 1232, 1378, 1458
MlyI GAGTC 2 cut(s) 122, 794
MnlI CCTC 1 cut(s) 367
Mph1103I ATGCAT 1 cut(s) 463
MroXI GAANNNNTTC 2 cut(s) 722, 884
MseI TTAA 5 cut(s) 327, 536, 867, 1058, 1260
MslI CAYNNNNRTG 7 cut(s) 66, 78, 402, 750, 1060, 1098, 1308
MspA1I CMGCKG 2 cut(s) 983, 1145
MspR9I CCNGG 1 cut(s) 1295
MunI CAATTG 1 cut(s) 249
MvaI CCWGG 1 cut(s) 1295
MvnI CGCG 1 cut(s) 983
MwoI GCNNNNNNNGC 4 cut(s) 207, 289, 638, 729
NcoI CCATGG 1 cut(s) 265
NlaIV GGNNCC 4 cut(s) 195, 743, 1388, 1489
NmuCI GTSAC 3 cut(s) 425, 675, 1396
NsbI TGCGCA 1 cut(s) 209
NsiI ATGCAT 1 cut(s) 463
NspI RCATGY 3 cut(s) 465, 1065, 1267
OliI CACNNNNGTG 1 cut(s) 1308
PaeI GCATGC 1 cut(s) 465
PagI TCATGA 1 cut(s) 778
PciI ACATGT 1 cut(s) 1061
PcsI WCGNNNNNNNCGW 1 cut(s) 64
PdmI GAANNNNTTC 2 cut(s) 722, 884
PfeI GAWTC 4 cut(s) 391, 577, 1240, 1300
PkrI GCNGC 5 cut(s) 562, 985, 1144, 1274, 1442
PleI GAGTC 2 cut(s) 121, 793
PpsI GAGTC 2 cut(s) 121, 793
PscI ACATGT 1 cut(s) 1061
Psp124BI GAGCTC 1 cut(s) 480
Psp6I CCWGG 1 cut(s) 1293
PspFI CCCAGC 1 cut(s) 197
PspGI CCWGG 1 cut(s) 1293
PspN4I GGNNCC 4 cut(s) 195, 743, 1388, 1489
PstI CTGCAG 1 cut(s) 565
PstNI CAGNNNCTG 2 cut(s) 574, 1156
PsuI RGATCY 4 cut(s) 571, 741, 1426, 1487
PvuII CAGCTG 1 cut(s) 1145
RsaI GTAC 2 cut(s) 153, 494
RsaNI GTAC 2 cut(s) 152, 493
RseI CAYNNNNRTG 7 cut(s) 66, 78, 402, 750, 1060, 1098, 1308
SacI GAGCTC 1 cut(s) 480
SacII CCGCGG 1 cut(s) 984
SalI GTCGAC 1 cut(s) 121
SaqAI TTAA 5 cut(s) 327, 536, 867, 1058, 1260
SatI GCNGC 5 cut(s) 561, 984, 1143, 1273, 1441
SchI GAGTC 2 cut(s) 122, 794
ScrFI CCNGG 1 cut(s) 1295
SduI GDGCHC 2 cut(s) 480, 521
SexAI ACCWGGT 1 cut(s) 1293
SfaNI GCATC 3 cut(s) 343, 448, 1159
SfcI CTRYAG 3 cut(s) 187, 561, 847
Sfr303I CCGCGG 1 cut(s) 984
SgrBI CCGCGG 1 cut(s) 984
SmiMI CAYNNNNRTG 7 cut(s) 66, 78, 402, 750, 1060, 1098, 1308
SmlI CTYRAG 2 cut(s) 818, 1405
SmoI CTYRAG 2 cut(s) 818, 1405
SpeI ACTAGT 1 cut(s) 241
SphI GCATGC 1 cut(s) 465
Sse9I AATT 7 cut(s) 249, 812, 832, 1183, 1232, 1378, 1458
SsiI CCGC 5 cut(s) 161, 553, 981, 983, 1129
SspMI CTAG 2 cut(s) 242, 1268
SstI GAGCTC 1 cut(s) 480
StyD4I CCNGG 1 cut(s) 1293
StyI CCWWGG 2 cut(s) 265, 545
TaaI ACNGT 7 cut(s) 15, 151, 675, 737, 851, 1219, 1391
TaqI TCGA 3 cut(s) 5, 122, 1323
TasI AATT 7 cut(s) 249, 812, 832, 1183, 1232, 1378, 1458
TatI WGTACW 1 cut(s) 151
TauI GCSGC 1 cut(s) 986
TfiI GAWTC 4 cut(s) 391, 577, 1240, 1300
Tru1I TTAA 5 cut(s) 327, 536, 867, 1058, 1260
Tru9I TTAA 5 cut(s) 327, 536, 867, 1058, 1260
TscAI CASTG 4 cut(s) 436, 895, 1222, 1290
TseFI GTSAC 3 cut(s) 425, 675, 1396
TseI GCWGC 4 cut(s) 560, 1142, 1272, 1440
Tsp45I GTSAC 3 cut(s) 425, 675, 1396
TspDTI ATGAA 4 cut(s) 386, 457, 503, 782
TspGWI ACGGA 6 cut(s) 56, 68, 74, 84, 1032, 1130
TspRI CASTG 4 cut(s) 436, 895, 1222, 1290
VneI GTGCAC 1 cut(s) 517
XapI RAATTY 3 cut(s) 812, 1232, 1378
XceI RCATGY 3 cut(s) 465, 1065, 1267
XmiI GTMKAC 1 cut(s) 122
XmnI GAANNNNTTC 2 cut(s) 722, 884
XspI CTAG 2 cut(s) 242, 1268
Zsp2I ATGCAT 1 cut(s) 463
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.