Rmu_sc0002357.1_g000013

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002357.1
OG0000821
Physical Location & Seq
Reverse (-)
41250 .. 44786
3537 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002357.1_g000013.1.cds

Sequence Viewer

Length: 1713 bp
atggtagaacctcagagtgaacaaagagagaatgcacccgtattgttcttgcaggaatggtgtatctataggtttcccagccagctccgcaatgtagatgaagcagcatacactcctcaaacactttcaatagggcctttccaccatggaaatccaaaactgagggacatggaggcccataagaacaaatattatgagttgttttgcacacgttcttcgaaaagtgaggacgaactaaggagcttcatgatggatcatcgagaaacaattctaaattgttatgcaggtacctttaaatgtgagatcgactttttgaacctcatcctagttgacgcctgcttcataattgaactctttttgactaattctgaacaagaacaacataatgcaaattattatatactgagatcaccgtggctgaggaaaacggtggagagggacctgatactgttggaaaatcagcttccttattttcttcttcaaaagctatatgactttgccatacctaccttttccaatggccatcttggcaatggagaccaagcacaagttgggggacatgtccataaagatagcaccaacagtcagcaatgctgcttcccttgcttccggcccggtgattttcaagagaactccatccgatctgaacctattcaacatggctttcttgaacttacctgtaagttttttaaggcatacaccaagggaaaatctgtcgggaatggatttgagccaaaacacttcactgatttggttagacactttctatgcccgtgcaaggagctgttgtgggtcaaaaattatgatcagcccgtcaaaattaaataccatgcaaaaaagctgaaggttgcaggagtgaagtttatgccacttagagaaccatttgttataaaggaagaccctgtgttgacgtttaacccagtatgtttggcacataaggagttgaagcttacaagactttgggcagagtatgcggcagaatgcatttttcgaaacaccatggccttggaacaactgatgtatccagaaaagccttatatttgctgttatttttcgctgatgaatcaactcattaatactgaggaagatgtggatgtcctgattgaggatggagtaatcttcaacttgctaggcagcaacaaagcagtggtaaacatgatcagttcattgtgtgagcagattaaggaggatcactcctgctataacagcccaacaagaagaaagccaaagcaagtcctatcacgccaccttctgaccccgatccacgccgagagcagtaggactccagtcgtcgccgaccagtgcctcctgcatcgcttggaattccgcctatcaccaccgctgacgcctacacaccccacgtccgatacaaatcagatgaccgccaaaccaaagccagcgaaccagatccacgaggaccacgacccagccacttctgacaagactacctcctcccccgcgagccccccatcatgcacaacctcgccgacgccgccaactcacgaaccacaatctaggctagaaacgatccagtcaacatgccgattcgccgagacgcttatttctgtcccaacccccacctcccacctcaataggggtagcagatcagagatgagactagaaagacccatccgacgacgacgccatgaaggctcgctgttggacggaacaagaaaagatggcggctattcctcaagagagagagagagatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

570

Amino Acids

65.67

Weight (kDa)

7.29

Isoelectric Point (pI)

57.66

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 890
Acc36I ACCTGC 1 cut(s) 275
Acc65I GGTACC 1 cut(s) 287
AccB1I GGYRCC 1 cut(s) 287
AccII CGCG 1 cut(s) 1460
AciI CCGC 8 cut(s) 88, 974, 1327, 1340, 1383, 1458, 1493, 1683
AclWI GGATC 5 cut(s) 261, 1197, 1255, 1402, 1522
AcoI YGGCCR 1 cut(s) 520
AcsI RAATTY 1 cut(s) 1322
AcuI CTGAAG 1 cut(s) 863
AcyI GRCGYC 4 cut(s) 333, 1346, 1490, 1642
AfaI GTAC 1 cut(s) 289
AfiI CCNNNNNNNGG 3 cut(s) 778, 1105, 1594
AflIII ACRYGT 2 cut(s) 209, 559
AgsI TTSAA 9 cut(s) 129, 316, 350, 482, 626, 656, 671, 946, 1123
AjiI CACGTC 1 cut(s) 1362
AluBI AGCT 7 cut(s) 85, 243, 463, 487, 784, 841, 949
AluI AGCT 7 cut(s) 85, 243, 463, 487, 784, 841, 949
Alw26I GTCTC 3 cut(s) 531, 1547, 1609
AlwI GGATC 5 cut(s) 261, 1197, 1255, 1402, 1522
AoxI GGCC 5 cut(s) 134, 174, 520, 611, 1002
ApeKI GCWGC 3 cut(s) 104, 594, 1134
ApoI RAATTY 1 cut(s) 1322
AseI ATTAAT 1 cut(s) 1074
Asp700I GAANNNNTTC 2 cut(s) 267, 651
Asp718I GGTACC 1 cut(s) 287
AspS9I GGNCC 5 cut(s) 134, 175, 439, 612, 1417
AsuC2I CCSGG 1 cut(s) 615
AsuHPI GGTGA 3 cut(s) 402, 629, 1326
AsuII TTCGAA 2 cut(s) 218, 991
AvaII GGWCC 2 cut(s) 439, 1417
BaeI ACNNNNGTAYC 2 cut(s) 1004, 1037
BalI TGGCCA 1 cut(s) 522
BanI GGYRCC 1 cut(s) 287
BanII GRGCYC 1 cut(s) 1466
BauI CACGAG 1 cut(s) 1412
BbsI GAAGAC 1 cut(s) 903
BbvCI CCTCAGC 1 cut(s) 419
BbvI GCAGC 3 cut(s) 116, 581, 1146
BccI CCATC 7 cut(s) 244, 531, 644, 1103, 1477, 1637, 1673
BciVI GTATCC 1 cut(s) 1032
BclI TGATCA 2 cut(s) 805, 1158
BcnI CCSGG 1 cut(s) 615
BcoDI GTCTC 3 cut(s) 531, 1547, 1609
BfaI CTAG 5 cut(s) 326, 1130, 1515, 1520, 1619
BfmI CTRYAG 1 cut(s) 67
BfuAI ACCTGC 1 cut(s) 275
BfuI GTATCC 1 cut(s) 1032
BglI GCCNNNNNGGC 2 cut(s) 528, 1650
BisI GCNGC 6 cut(s) 105, 595, 975, 1135, 1493, 1684
BlsI GCNGC 6 cut(s) 106, 596, 976, 1136, 1494, 1685
Bme1390I CCNGG 1 cut(s) 615
Bme18I GGWCC 2 cut(s) 439, 1417
BmgBI CACGTC 1 cut(s) 1362
BmgT120I GGNCC 5 cut(s) 134, 175, 439, 612, 1417
BmiI GGNNCC 2 cut(s) 289, 440
BmrFI CCNGG 1 cut(s) 615
BmrI ACTGGG 1 cut(s) 914
BmsI GCATC 1 cut(s) 1321
BmuI ACTGGG 1 cut(s) 914
BoxI GACNNNNGTC 1 cut(s) 1286
BpiI GAAGAC 1 cut(s) 903
BplI GAGNNNNNCTC 2 cut(s) 1178, 1210
BpmI CTGGAG 1 cut(s) 1269
Bpu10I CCTNAGC 1 cut(s) 419
Bpu14I TTCGAA 2 cut(s) 218, 991
BpuEI CTTGAG 1 cut(s) 1678
BpuMI CCSGG 1 cut(s) 615
BsaBI GATNNNNATC 1 cut(s) 1371
BsaHI GRCGYC 4 cut(s) 333, 1346, 1490, 1642
BsaI GGTCTC 1 cut(s) 531
BsaJI CCNNGG 5 cut(s) 145, 413, 702, 999, 1005
BsaXI ACNNNNNCTCC 2 cut(s) 773, 803
Bsc4I CCNNNNNNNGG 3 cut(s) 778, 1105, 1594
Bse1I ACTGG 4 cut(s) 920, 1286, 1300, 1531
Bse3DI GCAATG 3 cut(s) 97, 538, 596
Bse8I GATNNNNATC 1 cut(s) 1371
BseDI CCNNGG 5 cut(s) 145, 413, 702, 999, 1005
BseGI GGATG 5 cut(s) 321, 636, 1099, 1114, 1629
BseJI GATNNNNATC 1 cut(s) 1371
BseLI CCNNNNNNNGG 3 cut(s) 778, 1105, 1594
BseMI GCAATG 3 cut(s) 97, 538, 596
BseMII CTCAG 5 cut(s) 26, 152, 395, 410, 1071
BseNI ACTGG 4 cut(s) 920, 1286, 1300, 1531
BseRI GAGGAG 2 cut(s) 105, 1441
BseXI GCAGC 3 cut(s) 116, 581, 1146
BseYI CCCAGC 2 cut(s) 77, 1426
Bsh1236I CGCG 1 cut(s) 1460
BshFI GGCC 5 cut(s) 136, 176, 522, 613, 1004
BshNI GGYRCC 1 cut(s) 287
BsiSI CCGG 2 cut(s) 610, 615
BslFI GGGAC 4 cut(s) 179, 452, 570, 1553
BslI CCNNNNNNNGG 3 cut(s) 778, 1105, 1594
BsmAI GTCTC 3 cut(s) 531, 1547, 1609
BsmBI CGTCTC 1 cut(s) 1547
BsmFI GGGAC 4 cut(s) 179, 452, 570, 1553
BsmI GAATGC 2 cut(s) 37, 986
BsnI GGCC 5 cut(s) 136, 176, 522, 613, 1004
Bso31I GGTCTC 1 cut(s) 531
Bsp119I TTCGAA 2 cut(s) 218, 991
Bsp1286I GDGCHC 1 cut(s) 1466
Bsp19I CCATGG 2 cut(s) 145, 999
BspACI CCGC 8 cut(s) 88, 974, 1327, 1340, 1383, 1458, 1493, 1683
BspANI GGCC 5 cut(s) 136, 176, 522, 613, 1004
BspCNI CTCAG 5 cut(s) 25, 153, 396, 411, 1072
BspFNI CGCG 1 cut(s) 1460
BspHI TCATGA 1 cut(s) 246
BspLI GGNNCC 2 cut(s) 289, 440
BspMI ACCTGC 1 cut(s) 275
BspPI GGATC 5 cut(s) 261, 1197, 1255, 1402, 1522
BspT104I TTCGAA 2 cut(s) 218, 991
BspT107I GGYRCC 1 cut(s) 287
BspTNI GGTCTC 1 cut(s) 531
BsrDI GCAATG 3 cut(s) 97, 538, 596
BsrI ACTGG 4 cut(s) 920, 1286, 1300, 1531
BssECI CCNNGG 5 cut(s) 145, 413, 702, 999, 1005
BssNI GRCGYC 4 cut(s) 333, 1346, 1490, 1642
BssSI CACGAG 1 cut(s) 1412
BssT1I CCWWGG 4 cut(s) 145, 702, 999, 1005
Bst2BI CACGAG 1 cut(s) 1412
Bst4CI ACNGT 4 cut(s) 414, 430, 450, 584
BstACI GRCGYC 4 cut(s) 333, 1346, 1490, 1642
BstAPI GCANNNNNTGC 1 cut(s) 971
BstBI TTCGAA 2 cut(s) 218, 991
BstC8I GCNNGC 5 cut(s) 83, 337, 1398, 1462, 1655
BstDEI CTNAG 7 cut(s) 12, 161, 236, 404, 419, 872, 1080
BstDSI CCRYGG 3 cut(s) 145, 413, 999
BstENI CCTNNNNNAGG 1 cut(s) 1103
BstF5I GGATG 5 cut(s) 321, 636, 1099, 1114, 1629
BstFNI CGCG 1 cut(s) 1460
BstMAI GTCTC 3 cut(s) 531, 1547, 1609
BstMWI GCNNNNNNNGC 7 cut(s) 87, 528, 603, 971, 1206, 1492, 1650
BstNSI RCATGY 2 cut(s) 563, 1542
BstPAI GACNNNNGTC 1 cut(s) 1286
BstSCI CCNGG 1 cut(s) 613
BstSFI CTRYAG 1 cut(s) 67
BstUI CGCG 1 cut(s) 1460
BstV1I GCAGC 3 cut(s) 116, 581, 1146
BstV2I GAAGAC 1 cut(s) 903
BstX2I RGATCY 1 cut(s) 1407
BstXI CCANNNNNNTGG 1 cut(s) 1006
BstYI RGATCY 1 cut(s) 1407
BsuI GTATCC 1 cut(s) 1032
BsuRI GGCC 5 cut(s) 136, 176, 522, 613, 1004
BtgI CCRYGG 3 cut(s) 145, 413, 999
BtgZI GCGATG 1 cut(s) 1298
BtrI CACGTC 1 cut(s) 1362
BtsCI GGATG 5 cut(s) 321, 636, 1099, 1114, 1629
BtsI GCAGTG 1 cut(s) 1152
BtsIMutI CAGTG 3 cut(s) 744, 1152, 1307
BveI ACCTGC 1 cut(s) 275
Cac8I GCNNGC 5 cut(s) 83, 337, 1398, 1462, 1655
CciI TCATGA 1 cut(s) 246
Cfr13I GGNCC 5 cut(s) 134, 175, 439, 612, 1417
CseI GACGC 5 cut(s) 341, 1354, 1498, 1564, 1650
Csp6I GTAC 1 cut(s) 288
CviQI GTAC 1 cut(s) 288
DdeI CTNAG 7 cut(s) 12, 161, 236, 404, 419, 872, 1080
DraI TTTAAA 1 cut(s) 295
EaeI YGGCCR 1 cut(s) 520
EciI GGCGGA 1 cut(s) 1316
Eco130I CCWWGG 4 cut(s) 145, 702, 999, 1005
Eco24I GRGCYC 1 cut(s) 1466
Eco31I GGTCTC 1 cut(s) 531
Eco47I GGWCC 2 cut(s) 439, 1417
Eco57I CTGAAG 1 cut(s) 863
EcoNI CCTNNNNNAGG 1 cut(s) 1103
EcoO109I RGGNCCY 2 cut(s) 134, 439
EcoRI GAATTC 1 cut(s) 1322
EcoT14I CCWWGG 4 cut(s) 145, 702, 999, 1005
EcoT22I ATGCAT 1 cut(s) 986
EcoT38I GRGCYC 1 cut(s) 1466
ErhI CCWWGG 4 cut(s) 145, 702, 999, 1005
Esp3I CGTCTC 1 cut(s) 1547
FaqI GGGAC 4 cut(s) 179, 452, 570, 1553
FauI CCCGC 1 cut(s) 1465
FbaI TGATCA 2 cut(s) 805, 1158
Fnu4HI GCNGC 6 cut(s) 105, 595, 975, 1135, 1493, 1684
FokI GGATG 5 cut(s) 308, 623, 1106, 1121, 1616
FriOI GRGCYC 1 cut(s) 1466
Fsp4HI GCNGC 6 cut(s) 105, 595, 975, 1135, 1493, 1684
FspBI CTAG 5 cut(s) 326, 1130, 1515, 1520, 1619
GluI GCNGC 6 cut(s) 105, 595, 975, 1135, 1493, 1684
GsaI CCCAGC 2 cut(s) 81, 1430
GsuI CTGGAG 1 cut(s) 1269
HaeIII GGCC 5 cut(s) 136, 176, 522, 613, 1004
HapII CCGG 2 cut(s) 610, 615
HgaI GACGC 5 cut(s) 341, 1354, 1498, 1564, 1650
Hin1I GRCGYC 4 cut(s) 333, 1346, 1490, 1642
HincII GTYRAC 3 cut(s) 331, 909, 1536
HindII GTYRAC 3 cut(s) 331, 909, 1536
HindIII AAGCTT 1 cut(s) 947
HinfI GANTC 3 cut(s) 1063, 1282, 1545
HpaII CCGG 2 cut(s) 610, 615
HphI GGTGA 3 cut(s) 402, 629, 1326
Hpy166II GTNNAC 5 cut(s) 20, 331, 909, 1153, 1536
Hpy188III TCNNGA 9 cut(s) 247, 260, 626, 668, 718, 1025, 1099, 1502, 1695
Hpy8I GTNNAC 5 cut(s) 20, 331, 909, 1153, 1536
Hpy99I CGWCG 5 cut(s) 1295, 1492, 1638, 1641, 1644
HpyAV CCTTC 3 cut(s) 838, 1259, 1643
HpyCH4III ACNGT 4 cut(s) 414, 430, 450, 584
HpyCH4IV ACGT 3 cut(s) 211, 911, 1361
HpyF10VI GCNNNNNNNGC 7 cut(s) 87, 528, 603, 971, 1206, 1492, 1650
HpyF3I CTNAG 7 cut(s) 12, 161, 236, 404, 419, 872, 1080
HpySE526I ACGT 3 cut(s) 211, 911, 1361
Hsp92I GRCGYC 4 cut(s) 333, 1346, 1490, 1642
KpnI GGTACC 1 cut(s) 291
Ksp22I TGATCA 2 cut(s) 805, 1158
LmnI GCTCC 3 cut(s) 90, 240, 781
Lsp1109I GCAGC 3 cut(s) 116, 581, 1146
LweI GCATC 1 cut(s) 1321
MaeI CTAG 5 cut(s) 326, 1130, 1515, 1520, 1619
MaeII ACGT 3 cut(s) 211, 911, 1361
MboII GAAGA 7 cut(s) 207, 467, 470, 908, 1097, 1111, 1230
MflI RGATCY 1 cut(s) 1407
MhlI GDGCHC 1 cut(s) 1466
MlsI TGGCCA 1 cut(s) 522
MluCI AATT 8 cut(s) 267, 274, 345, 364, 391, 799, 819, 1322
MluNI TGGCCA 1 cut(s) 522
MlyI GAGTC 1 cut(s) 1276
MmeI TCCRAC 3 cut(s) 432, 1641, 1657
Mox20I TGGCCA 1 cut(s) 522
Mph1103I ATGCAT 1 cut(s) 986
MroXI GAANNNNTTC 2 cut(s) 267, 651
MscI TGGCCA 1 cut(s) 522
MseI TTAA 6 cut(s) 294, 690, 822, 915, 1074, 1182
Msp20I TGGCCA 1 cut(s) 522
MspA1I CMGCKG 1 cut(s) 1342
MspI CCGG 2 cut(s) 610, 615
MspR9I CCNGG 1 cut(s) 615
Mva1269I GAATGC 2 cut(s) 37, 986
MvnI CGCG 1 cut(s) 1460
MwoI GCNNNNNNNGC 7 cut(s) 87, 528, 603, 971, 1206, 1492, 1650
NciI CCSGG 1 cut(s) 615
NcoI CCATGG 2 cut(s) 145, 999
NlaIV GGNNCC 2 cut(s) 289, 440
NmeAIII GCCGAG 2 cut(s) 1294, 1576
NsiI ATGCAT 1 cut(s) 986
NspI RCATGY 2 cut(s) 563, 1542
NspV TTCGAA 2 cut(s) 218, 991
PagI TCATGA 1 cut(s) 246
PciI ACATGT 1 cut(s) 559
PcsI WCGNNNNNNNCGW 1 cut(s) 1419
PctI GAATGC 2 cut(s) 37, 986
PdmI GAANNNNTTC 2 cut(s) 267, 651
PfeI GAWTC 2 cut(s) 1063, 1545
PkrI GCNGC 6 cut(s) 106, 596, 976, 1136, 1494, 1685
PleI GAGTC 1 cut(s) 1276
PpsI GAGTC 1 cut(s) 1276
PpuMI RGGWCCY 1 cut(s) 439
PscI ACATGT 1 cut(s) 559
PshAI GACNNNNGTC 1 cut(s) 1286
PshBI ATTAAT 1 cut(s) 1074
PsiI TTATAA 1 cut(s) 890
Psp5II RGGWCCY 1 cut(s) 439
PspFI CCCAGC 2 cut(s) 77, 1426
PspN4I GGNNCC 2 cut(s) 289, 440
PspPI GGNCC 5 cut(s) 134, 175, 439, 612, 1417
PspPPI RGGWCCY 1 cut(s) 439
PsuI RGATCY 1 cut(s) 1407
RsaI GTAC 1 cut(s) 289
RsaNI GTAC 1 cut(s) 288
SaqAI TTAA 6 cut(s) 294, 690, 822, 915, 1074, 1182
SatI GCNGC 6 cut(s) 105, 595, 975, 1135, 1493, 1684
Sau96I GGNCC 5 cut(s) 134, 175, 439, 612, 1417
SchI GAGTC 1 cut(s) 1276
ScrFI CCNGG 1 cut(s) 615
SduI GDGCHC 1 cut(s) 1466
SfaNI GCATC 1 cut(s) 1321
SfcI CTRYAG 1 cut(s) 67
SfuI TTCGAA 2 cut(s) 218, 991
SinI GGWCC 2 cut(s) 439, 1417
SmlI CTYRAG 1 cut(s) 1693
SmoI CTYRAG 1 cut(s) 1693
Sse9I AATT 8 cut(s) 267, 274, 345, 364, 391, 799, 819, 1322
SsiI CCGC 8 cut(s) 88, 974, 1327, 1340, 1383, 1458, 1493, 1683
SspI AATATT 1 cut(s) 191
SspMI CTAG 5 cut(s) 326, 1130, 1515, 1520, 1619
StyD4I CCNGG 1 cut(s) 613
StyI CCWWGG 4 cut(s) 145, 702, 999, 1005
TaaI ACNGT 4 cut(s) 414, 430, 450, 584
TaiI ACGT 3 cut(s) 214, 914, 1364
TaqI TCGA 4 cut(s) 218, 259, 306, 991
TasI AATT 8 cut(s) 267, 274, 345, 364, 391, 799, 819, 1322
TauI GCSGC 3 cut(s) 977, 1495, 1686
TfiI GAWTC 2 cut(s) 1063, 1545
Tru1I TTAA 6 cut(s) 294, 690, 822, 915, 1074, 1182
Tru9I TTAA 6 cut(s) 294, 690, 822, 915, 1074, 1182
TscAI CASTG 3 cut(s) 751, 1152, 1307
TseI GCWGC 3 cut(s) 104, 594, 1134
TspDTI ATGAA 6 cut(s) 114, 235, 331, 1076, 1155, 1662
TspGWI ACGGA 1 cut(s) 1680
TspRI CASTG 3 cut(s) 751, 1152, 1307
VpaK11BI GGWCC 2 cut(s) 439, 1417
VspI ATTAAT 1 cut(s) 1074
XagI CCTNNNNNAGG 1 cut(s) 1103
XapI RAATTY 1 cut(s) 1322
XceI RCATGY 2 cut(s) 563, 1542
XcmI CCANNNNNNNNNTGG 2 cut(s) 530, 548
XmnI GAANNNNTTC 2 cut(s) 267, 651
XspI CTAG 5 cut(s) 326, 1130, 1515, 1520, 1619
Zsp2I ATGCAT 1 cut(s) 986
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.