Rroxscaffold_4G00308200

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000821
Physical Location & Seq
Forward (+)
29710815 .. 29712316
1502 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00308200.1

Sequence Viewer

Length: 1425 bp
ATGATTTTGTCAAAGTCGACTTCACAGCTGGAGGGTCTTAAAACTGGGATTGAAGCTTTACGAAGGTCGGACAAGCTGGACAAGTGGTGTATCTATAGGGTTCCCAGTAAACTGCGTGGTGTCAATGAAGCAGCATATACCCCCCAATTACTTTCAGTTGGCCTTTTCCACCATGGCAAAGCAGAACTGAAGGACATGGAGAACCATAAAACAGAATATGTGGAGTACTTTTTTGAGCGCACTCTGAAAGATAAGGCTGCAGTAAGAAGCTTCATTGAAGATCATAAAGAGAAAATTCTTCATTGTTATGCAGGGACAATTGAGCTCGGTGAAGGCATTGACCCTGTTGATATAATTCTAGTTGATGCATGCTTCATCATCGAGCTCTTTCTGAGGAGGTCTGGAAAAGAAAATGGTGATAAATACACAAAGGATTACATATTAAGTACACCTTGGGTGTGGAAAGCTACAGAGCAGGATCTGATACTGTTTGAAAATCAGCTTCCTTATTTTCTTCTTCAAGAACTATATGACTATGCATTTCCCGGAGCGCCTTCCATTTCCCATGCTGGAAAAGAAATACAATCACATAAATCCAGCACCAATAACAAGCGGAACTGCTTGGTTTGCTTCCAGGACTGCTTACCTTGCTACTGTTGGATCCATCCCAATGATCGTCATGTTATAAAGATGGATCATGAGTCCGAGCCTGAACGTATTCATGATCACTTTCTTGAGCTGGCCTGTAATTTTTTCAAAAATTACAGTGAGGGAAAATCTGTTAGGGTAAAACCAAAACATTTCACTGATTTGGTAAGATATTTTATGTGTCCAGAAGAAGCAATGGAGTGGAAAAAAAATACTACCAATTGTCAATACAATATGAGAATGCTGACGGCAGCAGGGGTTAACTTTATGCCATTTCCTAAAGCAGGACGCTTTGTGGTAAAAGAAGGTAAAAGTTTCAAGTTAACAATTTTTAAAGGAATGGATTTGAAGCTTTCACAGTTTTGTGTAAAGGATGAGACCGAATGTATTATTCGCAATGTCATGGCAGTTGAGCAGTTTCTGTATCCAGACGATGCTTATGTTTGCAATTACTTCTTGCTGATGGATAAACTTGTGGACACTGTTGAAGATGTGGATTTTCTGATTCATAAAAAAGTGATCGTTAACATGCTAGGCAGCAACAAAGCAGTGGTGGACCTGGTGAATCACCTATGTAATCAGATTCTCCGAGAAAGATCGTGCTATGTTCATATCTGTAAAGAACTAAATGAGCACTATGAGAATCGTTGGAACTGTCGCTTGACAAACCTGAAACAAGTTTACTTCAAGGATATGTGGACAGGCAGTTCAACTATTTTCGGAATTGTTGTTATGGGATTCTCTATCATTGGATCCATTAATTCATTCAAAAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

474

Amino Acids

55.22

Weight (kDa)

7.22

Isoelectric Point (pI)

39.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 31 - 461 4.3e-88 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 686
AccI GTMKAC 1 cut(s) 17
AciI CCGC 1 cut(s) 613
AclWI GGATC 6 cut(s) 486, 655, 668, 702, 1395, 1408
AcsI RAATTY 1 cut(s) 294
AcuI CTGAAG 1 cut(s) 209
AfaI GTAC 2 cut(s) 227, 448
AfiI CCNNNNNNNGG 1 cut(s) 932
AjnI CCWGG 2 cut(s) 633, 1206
AloI GAACNNNNNNTCC 2 cut(s) 1339, 1371
Alw21I GWGCWC 3 cut(s) 327, 387, 1284
Alw26I GTCTC 1 cut(s) 1019
AlwI GGATC 6 cut(s) 486, 655, 668, 702, 1395, 1408
AlwNI CAGNNNCTG 2 cut(s) 481, 1069
AoxI GGCC 2 cut(s) 160, 741
ApeKI GCWGC 4 cut(s) 131, 257, 899, 1185
ApoI RAATTY 1 cut(s) 294
AseI ATTAAT 1 cut(s) 1407
Asp700I GAANNNNTTC 1 cut(s) 717
AspLEI GCGC 2 cut(s) 240, 553
AspS9I GGNCC 1 cut(s) 1204
AsuC2I CCSGG 1 cut(s) 546
AsuHPI GGTGA 4 cut(s) 341, 428, 1208, 1222
AvaII GGWCC 1 cut(s) 1204
BamHI GGATCC 2 cut(s) 660, 1400
BanII GRGCYC 2 cut(s) 327, 387
Bbv12I GWGCWC 3 cut(s) 327, 387, 1284
BbvI GCAGC 4 cut(s) 143, 244, 911, 1197
BccI CCATC 3 cut(s) 672, 685, 1105
BceAI ACGGC 1 cut(s) 912
BciT130I CCWGG 2 cut(s) 635, 1208
BciVI GTATCC 1 cut(s) 1083
BclI TGATCA 1 cut(s) 724
BcnI CCSGG 1 cut(s) 546
BcoDI GTCTC 1 cut(s) 1019
BfaI CTAG 2 cut(s) 359, 1181
BfmI CTRYAG 3 cut(s) 94, 258, 468
BfoI RGCGCY 1 cut(s) 554
BfuI GTATCC 1 cut(s) 1083
BisI GCNGC 4 cut(s) 132, 258, 900, 1186
BlsI GCNGC 4 cut(s) 133, 259, 901, 1187
BmcAI AGTACT 1 cut(s) 227
Bme1390I CCNGG 3 cut(s) 546, 635, 1208
Bme18I GGWCC 1 cut(s) 1204
BmgT120I GGNCC 1 cut(s) 1204
BmiI GGNNCC 3 cut(s) 102, 662, 1402
BmrFI CCNGG 3 cut(s) 546, 635, 1208
BmrI ACTGGG 2 cut(s) 54, 99
BmsI GCATC 2 cut(s) 355, 1072
BmuI ACTGGG 2 cut(s) 54, 99
BpmI CTGGAG 1 cut(s) 50
BpuEI CTTGAG 1 cut(s) 755
BpuMI CCSGG 1 cut(s) 546
BsaI GGTCTC 1 cut(s) 1019
BsaJI CCNNGG 2 cut(s) 172, 452
Bsc4I CCNNNNNNNGG 1 cut(s) 932
Bse1I ACTGG 2 cut(s) 49, 105
Bse3DI GCAATG 2 cut(s) 849, 1051
BseBI CCWGG 2 cut(s) 635, 1208
BseDI CCNNGG 2 cut(s) 172, 452
BseGI GGATG 2 cut(s) 664, 1027
BseLI CCNNNNNNNGG 1 cut(s) 932
BseMI GCAATG 2 cut(s) 849, 1051
BseMII CTCAG 1 cut(s) 383
BseNI ACTGG 2 cut(s) 49, 105
BseRI GAGGAG 1 cut(s) 409
BseXI GCAGC 4 cut(s) 143, 244, 911, 1197
BshFI GGCC 2 cut(s) 162, 743
BsiHKAI GWGCWC 3 cut(s) 327, 387, 1284
BsiSI CCGG 1 cut(s) 546
BslFI GGGAC 1 cut(s) 328
BslI CCNNNNNNNGG 1 cut(s) 932
BsmAI GTCTC 1 cut(s) 1019
BsmFI GGGAC 1 cut(s) 328
BsmI GAATGC 1 cut(s) 894
BsnI GGCC 2 cut(s) 162, 743
Bso31I GGTCTC 1 cut(s) 1019
Bsp1286I GDGCHC 3 cut(s) 327, 387, 1284
Bsp143I GATC 9 cut(s) 280, 478, 660, 673, 694, 724, 1167, 1244, 1400
Bsp19I CCATGG 1 cut(s) 172
BspACI CCGC 1 cut(s) 613
BspANI GGCC 2 cut(s) 162, 743
BspCNI CTCAG 1 cut(s) 384
BspHI TCATGA 2 cut(s) 697, 721
BspLI GGNNCC 3 cut(s) 102, 662, 1402
BspMAI CTGCAG 1 cut(s) 262
BspPI GGATC 6 cut(s) 486, 655, 668, 702, 1395, 1408
BspTNI GGTCTC 1 cut(s) 1019
BsrDI GCAATG 2 cut(s) 849, 1051
BsrI ACTGG 2 cut(s) 49, 105
BssECI CCNNGG 2 cut(s) 172, 452
BssMI GATC 9 cut(s) 280, 478, 660, 673, 694, 724, 1167, 1244, 1400
BssT1I CCWWGG 2 cut(s) 172, 452
Bst2UI CCWGG 2 cut(s) 635, 1208
Bst4CI ACNGT 6 cut(s) 489, 656, 767, 1008, 1132, 1304
BstC8I GCNNGC 2 cut(s) 370, 741
BstDEI CTNAG 1 cut(s) 392
BstDSI CCRYGG 1 cut(s) 172
BstENI CCTNNNNNAGG 1 cut(s) 930
BstF5I GGATG 2 cut(s) 664, 1027
BstH2I RGCGCY 1 cut(s) 554
BstHHI GCGC 2 cut(s) 240, 553
BstKTI GATC 9 cut(s) 283, 481, 663, 676, 697, 727, 1170, 1247, 1403
BstMAI GTCTC 1 cut(s) 1019
BstMBI GATC 9 cut(s) 280, 478, 660, 673, 694, 724, 1167, 1244, 1400
BstMWI GCNNNNNNNGC 2 cut(s) 627, 648
BstNI CCWGG 2 cut(s) 635, 1208
BstNSI RCATGY 2 cut(s) 372, 1180
BstSCI CCNGG 3 cut(s) 544, 633, 1206
BstSFI CTRYAG 3 cut(s) 94, 258, 468
BstV1I GCAGC 4 cut(s) 143, 244, 911, 1197
BstX2I RGATCY 3 cut(s) 478, 660, 1400
BstYI RGATCY 3 cut(s) 478, 660, 1400
BsuI GTATCC 1 cut(s) 1083
BsuRI GGCC 2 cut(s) 162, 743
BtgI CCRYGG 1 cut(s) 172
BtsCI GGATG 2 cut(s) 664, 1027
BtsI GCAGTG 1 cut(s) 1203
BtsIMutI CAGTG 4 cut(s) 772, 804, 1128, 1203
Cac8I GCNNGC 2 cut(s) 370, 741
CaiI CAGNNNCTG 2 cut(s) 481, 1069
CciI TCATGA 2 cut(s) 697, 721
CfoI GCGC 2 cut(s) 240, 553
Cfr13I GGNCC 1 cut(s) 1204
CseI GACGC 1 cut(s) 945
CsiI ACCWGGT 1 cut(s) 1206
Csp6I GTAC 2 cut(s) 226, 447
CviAII CATG 9 cut(s) 173, 196, 369, 566, 680, 698, 722, 1051, 1177
CviQI GTAC 2 cut(s) 226, 447
DdeI CTNAG 1 cut(s) 392
DpnI GATC 9 cut(s) 282, 480, 662, 675, 696, 726, 1169, 1246, 1402
DpnII GATC 9 cut(s) 280, 478, 660, 673, 694, 724, 1167, 1244, 1400
DraI TTTAAA 1 cut(s) 982
Ecl136II GAGCTC 2 cut(s) 325, 385
Eco130I CCWWGG 2 cut(s) 172, 452
Eco24I GRGCYC 2 cut(s) 327, 387
Eco31I GGTCTC 1 cut(s) 1019
Eco47I GGWCC 1 cut(s) 1204
Eco53kI GAGCTC 2 cut(s) 325, 385
Eco57I CTGAAG 1 cut(s) 209
EcoICRI GAGCTC 2 cut(s) 325, 385
EcoNI CCTNNNNNAGG 1 cut(s) 930
EcoRII CCWGG 2 cut(s) 633, 1206
EcoT14I CCWWGG 2 cut(s) 172, 452
EcoT22I ATGCAT 2 cut(s) 370, 541
EcoT38I GRGCYC 2 cut(s) 327, 387
ErhI CCWWGG 2 cut(s) 172, 452
FaeI CATG 9 cut(s) 176, 199, 372, 569, 683, 701, 725, 1054, 1180
FalI AAGNNNNNCTT 2 cut(s) 436, 468
FaqI GGGAC 1 cut(s) 328
FatI CATG 9 cut(s) 172, 195, 368, 565, 679, 697, 721, 1050, 1176
FbaI TGATCA 1 cut(s) 724
FblI GTMKAC 1 cut(s) 17
Fnu4HI GCNGC 4 cut(s) 132, 258, 900, 1186
FokI GGATG 2 cut(s) 651, 1034
FriOI GRGCYC 2 cut(s) 327, 387
Fsp4HI GCNGC 4 cut(s) 132, 258, 900, 1186
FspBI CTAG 2 cut(s) 359, 1181
GlaI GCGC 2 cut(s) 239, 552
GluI GCNGC 4 cut(s) 132, 258, 900, 1186
GsuI CTGGAG 1 cut(s) 50
HaeII RGCGCY 1 cut(s) 554
HaeIII GGCC 2 cut(s) 162, 743
HapII CCGG 1 cut(s) 546
HgaI GACGC 1 cut(s) 945
HhaI GCGC 2 cut(s) 240, 553
Hin1II CATG 9 cut(s) 176, 199, 372, 569, 683, 701, 725, 1054, 1180
Hin6I GCGC 2 cut(s) 238, 551
HinP1I GCGC 2 cut(s) 238, 551
HincII GTYRAC 4 cut(s) 18, 910, 972, 1174
HindII GTYRAC 4 cut(s) 18, 910, 972, 1174
HindIII AAGCTT 3 cut(s) 54, 268, 998
HinfI GANTC 6 cut(s) 701, 1153, 1213, 1231, 1291, 1386
HpaI GTTAAC 3 cut(s) 910, 972, 1174
HpaII CCGG 1 cut(s) 546
HphI GGTGA 4 cut(s) 341, 428, 1208, 1222
Hpy188I TCNGA 9 cut(s) 70, 246, 393, 483, 706, 1152, 1230, 1238, 1370
Hpy188III TCNNGA 7 cut(s) 402, 521, 698, 722, 734, 833, 1076
HpyAV CCTTC 5 cut(s) 57, 184, 326, 564, 947
HpyCH4III ACNGT 6 cut(s) 489, 656, 767, 1008, 1132, 1304
HpyCH4IV ACGT 1 cut(s) 715
HpyCH4V TGCA 5 cut(s) 260, 311, 368, 539, 1095
HpyF10VI GCNNNNNNNGC 2 cut(s) 627, 648
HpyF3I CTNAG 1 cut(s) 392
HpySE526I ACGT 1 cut(s) 715
Hsp92II CATG 9 cut(s) 176, 199, 372, 569, 683, 701, 725, 1054, 1180
HspAI GCGC 2 cut(s) 238, 551
Ksp22I TGATCA 1 cut(s) 724
KspAI GTTAAC 3 cut(s) 910, 972, 1174
Kzo9I GATC 9 cut(s) 280, 478, 660, 673, 694, 724, 1167, 1244, 1400
LmnI GCTCC 1 cut(s) 548
Lsp1109I GCAGC 4 cut(s) 143, 244, 911, 1197
LweI GCATC 2 cut(s) 355, 1072
MabI ACCWGGT 1 cut(s) 1206
MaeI CTAG 2 cut(s) 359, 1181
MaeII ACGT 1 cut(s) 715
MalI GATC 9 cut(s) 282, 480, 662, 675, 696, 726, 1169, 1246, 1402
MboI GATC 9 cut(s) 280, 478, 660, 673, 694, 724, 1167, 1244, 1400
MboII GAAGA 6 cut(s) 290, 290, 506, 509, 848, 1148
MfeI CAATTG 2 cut(s) 318, 868
MflI RGATCY 3 cut(s) 478, 660, 1400
MhlI GDGCHC 3 cut(s) 327, 387, 1284
MlyI GAGTC 1 cut(s) 710
MmeI TCCRAC 3 cut(s) 48, 638, 1277
MnlI CCTC 4 cut(s) 25, 387, 390, 763
Mph1103I ATGCAT 2 cut(s) 370, 541
MroXI GAANNNNTTC 1 cut(s) 717
MseI TTAA 7 cut(s) 39, 443, 909, 971, 981, 1173, 1407
MslI CAYNNNNRTG 2 cut(s) 306, 669
MspA1I CMGCKG 1 cut(s) 28
MspI CCGG 1 cut(s) 546
MspR9I CCNGG 3 cut(s) 546, 635, 1208
MunI CAATTG 2 cut(s) 318, 868
Mva1269I GAATGC 1 cut(s) 894
MvaI CCWGG 2 cut(s) 635, 1208
MwoI GCNNNNNNNGC 2 cut(s) 627, 648
NciI CCSGG 1 cut(s) 546
NcoI CCATGG 1 cut(s) 172
NdeII GATC 9 cut(s) 280, 478, 660, 673, 694, 724, 1167, 1244, 1400
NlaIII CATG 9 cut(s) 176, 199, 372, 569, 683, 701, 725, 1054, 1180
NlaIV GGNNCC 3 cut(s) 102, 662, 1402
NsiI ATGCAT 2 cut(s) 370, 541
NspI RCATGY 2 cut(s) 372, 1180
PaeI GCATGC 1 cut(s) 372
PagI TCATGA 2 cut(s) 697, 721
PctI GAATGC 1 cut(s) 894
PdmI GAANNNNTTC 1 cut(s) 717
PfeI GAWTC 5 cut(s) 1153, 1213, 1231, 1291, 1386
PfoI TCCNGGA 2 cut(s) 544, 633
PkrI GCNGC 4 cut(s) 133, 259, 901, 1187
PleI GAGTC 1 cut(s) 709
PpsI GAGTC 1 cut(s) 709
PshBI ATTAAT 1 cut(s) 1407
PsiI TTATAA 1 cut(s) 686
Psp124BI GAGCTC 2 cut(s) 327, 387
Psp6I CCWGG 2 cut(s) 633, 1206
PspGI CCWGG 2 cut(s) 633, 1206
PspN4I GGNNCC 3 cut(s) 102, 662, 1402
PspPI GGNCC 1 cut(s) 1204
PstI CTGCAG 1 cut(s) 262
PstNI CAGNNNCTG 2 cut(s) 481, 1069
PsuI RGATCY 3 cut(s) 478, 660, 1400
PvuII CAGCTG 1 cut(s) 28
RsaI GTAC 2 cut(s) 227, 448
RsaNI GTAC 2 cut(s) 226, 447
RseI CAYNNNNRTG 2 cut(s) 306, 669
SacI GAGCTC 2 cut(s) 327, 387
SalI GTCGAC 1 cut(s) 16
SaqAI TTAA 7 cut(s) 39, 443, 909, 971, 981, 1173, 1407
SatI GCNGC 4 cut(s) 132, 258, 900, 1186
Sau3AI GATC 9 cut(s) 280, 478, 660, 673, 694, 724, 1167, 1244, 1400
Sau96I GGNCC 1 cut(s) 1204
ScaI AGTACT 1 cut(s) 227
SchI GAGTC 1 cut(s) 710
ScrFI CCNGG 3 cut(s) 546, 635, 1208
SduI GDGCHC 3 cut(s) 327, 387, 1284
SexAI ACCWGGT 1 cut(s) 1206
SfaNI GCATC 2 cut(s) 355, 1072
SfcI CTRYAG 3 cut(s) 94, 258, 468
SinI GGWCC 1 cut(s) 1204
SmiMI CAYNNNNRTG 2 cut(s) 306, 669
SmlI CTYRAG 1 cut(s) 734
SmoI CTYRAG 1 cut(s) 734
SphI GCATGC 1 cut(s) 372
SsiI CCGC 1 cut(s) 613
SspMI CTAG 2 cut(s) 359, 1181
SstI GAGCTC 2 cut(s) 327, 387
StyD4I CCNGG 3 cut(s) 544, 633, 1206
StyI CCWWGG 2 cut(s) 172, 452
TaaI ACNGT 6 cut(s) 489, 656, 767, 1008, 1132, 1304
TaiI ACGT 1 cut(s) 718
TaqI TCGA 2 cut(s) 17, 381
TaqII GACCGA 1 cut(s) 1043
TatI WGTACW 2 cut(s) 225, 446
TfiI GAWTC 5 cut(s) 1153, 1213, 1231, 1291, 1386
Tru1I TTAA 7 cut(s) 39, 443, 909, 971, 981, 1173, 1407
Tru9I TTAA 7 cut(s) 39, 443, 909, 971, 981, 1173, 1407
TscAI CASTG 4 cut(s) 772, 811, 1135, 1203
TseI GCWGC 4 cut(s) 131, 257, 899, 1185
TspDTI ATGAA 8 cut(s) 141, 262, 290, 364, 710, 1145, 1247, 1401
TspRI CASTG 4 cut(s) 772, 811, 1135, 1203
VpaK11BI GGWCC 1 cut(s) 1204
VspI ATTAAT 1 cut(s) 1407
XagI CCTNNNNNAGG 1 cut(s) 930
XapI RAATTY 1 cut(s) 294
XceI RCATGY 2 cut(s) 372, 1180
XmiI GTMKAC 1 cut(s) 17
XmnI GAANNNNTTC 1 cut(s) 717
XspI CTAG 2 cut(s) 359, 1181
ZrmI AGTACT 1 cut(s) 227
Zsp2I ATGCAT 2 cut(s) 370, 541
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.