pycom02g17910

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0000821
Physical Location & Seq
Forward (+)
16165392 .. 16165940
549 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g17910.1

Sequence Viewer

Length: 549 bp
ATGACTTTGCGGTGCCAGCCTCCAGTTCCCCTCATTCCAAAGAACGCTCAGGTGATCATTGTATTGATGTTTCAGAAGCTGAACATCTCCTTGATAGTACAGATGTCCCAGAAGCTGAACCTGTCACTGATCGATCCCTTTCTCAAGCTTACCTGCGAGTTCTTCAAAGATTACAGCAAGGGAAAATCCGTCAAGAATGTGGTAAAACCAAAACACTTCACTGATTTGGTCAGACATTTTCTCTGTCCCGAAGAAGAAATGACCTTTGTACATAATGCTTGTCCTATAAAAAATATATATGCTGTAAGGAAGCTCAAGGCAGCAGGGGTGAAGTTTACGCCACTTCATGAAGACGGATACTTTATCATAAAAGGAGACAAGCCTACTAAATGCAAGTTTAACTTGGCGTGTTTTCGAAATATGAACTTAAAGCTTGCACAATTTTGTGTCAAGGATGAGACAGAGTTCGTTATTCGAAACATCATGGCCTTGGAGCAGTTTTTGTACCCGGACAATCCTTATGAGGCGACCCTGAAACAAGCACTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

183

Amino Acids

21.04

Weight (kDa)

9.3

Isoelectric Point (pI)

34.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 47 - 170 1e-14 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 161
AccB1I GGYRCC 1 cut(s) 12
AciI CCGC 1 cut(s) 10
AclWI GGATC 1 cut(s) 128
AfaI GTAC 3 cut(s) 99, 270, 506
AgsI TTSAA 1 cut(s) 166
AluBI AGCT 5 cut(s) 79, 115, 148, 313, 433
AluI AGCT 5 cut(s) 79, 115, 148, 313, 433
Alw26I GTCTC 2 cut(s) 369, 452
AlwI GGATC 1 cut(s) 128
AlwNI CAGNNNCTG 2 cut(s) 79, 115
AoxI GGCC 1 cut(s) 486
ApeKI GCWGC 1 cut(s) 320
AsuC2I CCSGG 1 cut(s) 509
AsuHPI GGTGA 2 cut(s) 64, 340
AsuII TTCGAA 2 cut(s) 415, 475
BanI GGYRCC 1 cut(s) 12
BbsI GAAGAC 1 cut(s) 357
BbvI GCAGC 1 cut(s) 332
BciVI GTATCC 1 cut(s) 350
BclI TGATCA 1 cut(s) 54
BcnI CCSGG 1 cut(s) 509
BcoDI GTCTC 2 cut(s) 369, 452
BfuAI ACCTGC 1 cut(s) 161
BfuI GTATCC 1 cut(s) 350
BisI GCNGC 1 cut(s) 321
BlsI GCNGC 1 cut(s) 322
Bme1390I CCNGG 1 cut(s) 509
BmiI GGNNCC 1 cut(s) 14
BmrFI CCNGG 1 cut(s) 509
BpiI GAAGAC 1 cut(s) 357
BpmI CTGGAG 1 cut(s) 6
Bpu10I CCTNAGC 1 cut(s) 48
Bpu14I TTCGAA 2 cut(s) 415, 475
BpuEI CTTGAG 2 cut(s) 128, 299
BpuMI CCSGG 1 cut(s) 509
Bsa29I ATCGAT 1 cut(s) 132
BsaJI CCNNGG 1 cut(s) 489
Bse1I ACTGG 1 cut(s) 23
BseCI ATCGAT 1 cut(s) 132
BseDI CCNNGG 1 cut(s) 489
BseGI GGATG 1 cut(s) 460
BseMII CTCAG 1 cut(s) 62
BseNI ACTGG 1 cut(s) 23
BseXI GCAGC 1 cut(s) 332
BshFI GGCC 1 cut(s) 488
BshNI GGYRCC 1 cut(s) 12
BshVI ATCGAT 1 cut(s) 132
BsiSI CCGG 1 cut(s) 509
BslFI GGGAC 2 cut(s) 91, 231
BsmAI GTCTC 2 cut(s) 369, 452
BsmFI GGGAC 2 cut(s) 91, 231
BsnI GGCC 1 cut(s) 488
Bsp119I TTCGAA 2 cut(s) 415, 475
Bsp1407I TGTACA 1 cut(s) 268
Bsp143I GATC 3 cut(s) 54, 129, 133
BspACI CCGC 1 cut(s) 10
BspANI GGCC 1 cut(s) 488
BspCNI CTCAG 1 cut(s) 61
BspDI ATCGAT 1 cut(s) 132
BspHI TCATGA 1 cut(s) 346
BspLI GGNNCC 1 cut(s) 14
BspMI ACCTGC 1 cut(s) 161
BspPI GGATC 1 cut(s) 128
BspT104I TTCGAA 2 cut(s) 415, 475
BspT107I GGYRCC 1 cut(s) 12
BsrGI TGTACA 1 cut(s) 268
BsrI ACTGG 1 cut(s) 23
BssECI CCNNGG 1 cut(s) 489
BssMI GATC 3 cut(s) 54, 129, 133
BssT1I CCWWGG 1 cut(s) 489
BstAUI TGTACA 1 cut(s) 268
BstBI TTCGAA 2 cut(s) 415, 475
BstC8I GCNNGC 2 cut(s) 17, 435
BstDEI CTNAG 1 cut(s) 48
BstF5I GGATG 1 cut(s) 460
BstKTI GATC 3 cut(s) 57, 132, 136
BstMAI GTCTC 2 cut(s) 369, 452
BstMBI GATC 3 cut(s) 54, 129, 133
BstMWI GCNNNNNNNGC 1 cut(s) 16
BstSCI CCNGG 1 cut(s) 507
BstV1I GCAGC 1 cut(s) 332
BstV2I GAAGAC 1 cut(s) 357
Bsu15I ATCGAT 1 cut(s) 132
BsuI GTATCC 1 cut(s) 350
BsuRI GGCC 1 cut(s) 488
BsuTUI ATCGAT 1 cut(s) 132
BtsCI GGATG 1 cut(s) 460
BtsIMutI CAGTG 2 cut(s) 125, 219
BveI ACCTGC 1 cut(s) 161
Cac8I GCNNGC 2 cut(s) 17, 435
CaiI CAGNNNCTG 2 cut(s) 79, 115
CciI TCATGA 1 cut(s) 346
ClaI ATCGAT 1 cut(s) 132
Csp6I GTAC 3 cut(s) 98, 269, 505
CviAII CATG 2 cut(s) 347, 484
CviJI RGCY 8 cut(s) 19, 79, 115, 148, 313, 382, 433, 488
CviKI_1 RGCY 8 cut(s) 19, 79, 115, 148, 313, 382, 433, 488
CviQI GTAC 3 cut(s) 98, 269, 505
DdeI CTNAG 1 cut(s) 48
DpnI GATC 3 cut(s) 56, 131, 135
DpnII GATC 3 cut(s) 54, 129, 133
Eco130I CCWWGG 1 cut(s) 489
EcoT14I CCWWGG 1 cut(s) 489
ErhI CCWWGG 1 cut(s) 489
FaeI CATG 2 cut(s) 350, 487
FalI AAGNNNNNCTT 2 cut(s) 386, 418
FaqI GGGAC 2 cut(s) 91, 231
FatI CATG 2 cut(s) 346, 483
FbaI TGATCA 1 cut(s) 54
Fnu4HI GCNGC 1 cut(s) 321
FokI GGATG 1 cut(s) 467
Fsp4HI GCNGC 1 cut(s) 321
GluI GCNGC 1 cut(s) 321
GsuI CTGGAG 1 cut(s) 6
HaeIII GGCC 1 cut(s) 488
HapII CCGG 1 cut(s) 509
Hin1II CATG 2 cut(s) 350, 487
HindIII AAGCTT 2 cut(s) 146, 431
HpaII CCGG 1 cut(s) 509
HphI GGTGA 2 cut(s) 64, 340
Hpy166II GTNNAC 1 cut(s) 336
Hpy188I TCNGA 2 cut(s) 75, 233
Hpy188III TCNNGA 3 cut(s) 193, 248, 347
Hpy8I GTNNAC 1 cut(s) 336
HpyCH4V TGCA 2 cut(s) 393, 437
HpyF10VI GCNNNNNNNGC 1 cut(s) 16
HpyF3I CTNAG 1 cut(s) 48
Hsp92II CATG 2 cut(s) 350, 487
Ksp22I TGATCA 1 cut(s) 54
Kzo9I GATC 3 cut(s) 54, 129, 133
LmnI GCTCC 1 cut(s) 493
LpnPI CCDG 9 cut(s) 29, 35, 36, 122, 134, 166, 309, 522, 545
Lsp1109I GCAGC 1 cut(s) 332
MaeIII GTNAC 1 cut(s) 123
MalI GATC 3 cut(s) 56, 131, 135
MboI GATC 3 cut(s) 54, 129, 133
MboII GAAGA 4 cut(s) 154, 263, 266, 362
MluCI AATT 1 cut(s) 440
MnlI CCTC 3 cut(s) 30, 41, 517
MseI TTAA 2 cut(s) 399, 428
MspI CCGG 1 cut(s) 509
MspR9I CCNGG 1 cut(s) 509
MwoI GCNNNNNNNGC 1 cut(s) 16
NciI CCSGG 1 cut(s) 509
NdeII GATC 3 cut(s) 54, 129, 133
NlaIII CATG 2 cut(s) 350, 487
NlaIV GGNNCC 1 cut(s) 14
NmuCI GTSAC 1 cut(s) 123
NspV TTCGAA 2 cut(s) 415, 475
PagI TCATGA 1 cut(s) 346
PkrI GCNGC 1 cut(s) 322
PspN4I GGNNCC 1 cut(s) 14
PstNI CAGNNNCTG 2 cut(s) 79, 115
RsaI GTAC 3 cut(s) 99, 270, 506
RsaNI GTAC 3 cut(s) 98, 269, 505
SaqAI TTAA 2 cut(s) 399, 428
SatI GCNGC 1 cut(s) 321
Sau3AI GATC 3 cut(s) 54, 129, 133
ScrFI CCNGG 1 cut(s) 509
SetI ASST 9 cut(s) 54, 81, 117, 123, 150, 155, 266, 315, 435
SfuI TTCGAA 2 cut(s) 415, 475
SmlI CTYRAG 2 cut(s) 143, 314
SmoI CTYRAG 2 cut(s) 143, 314
Sse9I AATT 1 cut(s) 440
SsiI CCGC 1 cut(s) 10
StyD4I CCNGG 1 cut(s) 507
StyI CCWWGG 1 cut(s) 489
TaqI TCGA 3 cut(s) 132, 415, 475
TasI AATT 1 cut(s) 440
TatI WGTACW 2 cut(s) 97, 268
Tru1I TTAA 2 cut(s) 399, 428
Tru9I TTAA 2 cut(s) 399, 428
TscAI CASTG 2 cut(s) 132, 226
TseFI GTSAC 1 cut(s) 123
TseI GCWGC 1 cut(s) 320
Tsp45I GTSAC 1 cut(s) 123
TspDTI ATGAA 3 cut(s) 335, 363, 437
TspGWI ACGGA 2 cut(s) 178, 369
TspRI CASTG 2 cut(s) 132, 226
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.