Rroxscaffold_5G00334860

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0000821
Physical Location & Seq
Reverse (-)
2190753 .. 2192436
1684 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00334860.1

Sequence Viewer

Length: 1329 bp
ATGGTAGAACCAACTGTTCAAAGCGAACAAATAGAGAATGCACCCGTATTGTTCTCGCAGGAATGGTGTATCTATAGGGTTCCCAGCCAGCTCCGCAATGTAGATGAAGCAGCATACACTCCTCAAACACTTTCAATAGGGCCTTTCCACCATGGAAATCCAAAACTGAGGGACATGGTGGCCCATAAGAACAAATATTATGAGTTGTTTTGCAGACGTTCTGGGAAAAGTGAGGATGAGCTAAGGAGCTTCATGATGGATCATCGAGAAACAGTTCTAAATTGTTATGCCGGTACCATTAATTGTGACATCGACTTTTTGAAACTCATCCTAGTTGACGCCTGCTTCATAATTGAACTCTTTTTGACTAATTCTGAACAAGAACAACATTGTGCACATTATTATATACTGAGATCACCGTGGCTGAGGAAAACGGTGGAGAGGGACCTGATACTGTTGGAAAATCAGCTTCCTTATTTTCTTCTTCAAAAGCTATATGACTTTGCCATACCTACCTTTTCCAATGGCCATCTTGGCAATGGAGACCAAGCACAAGGACATGCTCATAAAGATAGCACCAACAGTCAGCAATGCTGCTTCCCTTGCTTCCGGCCCGGTGATTTTCAAGAGAACCCCATCCAATCTGAACCTATTCAACATGGCTTTCTTGAACTTACCTGTAAGTTTTTTAAGGCATACACTAAGGGAAAATCTGTCAGGAATGGATTTGAGCCGAAACACTTCACTGATTTGGTTAGACACTTTCTATGTCCATACAAGGAACTGTTGTGGGTAAAAAATTATGATCAGCCCGTCAAAATTAAATACCATGCAACAAAGCTGAAGGTTGCAGGAGTGAAGTTTGTGCCACTTAGAGAACCATTTGTTATAAAGGAAGACCCTGTGTTGACGTTTAACTCAGCATGTTTGACACATAAGGAGTTGAAGCTTACAAGACTTTGGGCAGAGTATGAGGCAGGATGCATTTTTCGAAACACCATGGCCTTGGAACAACTTATGTATCCAGAAAAGCCTTATATTTGCAGTTATTTTTCGCTGATGAATCAACTCATTAACTCGGAGGAAGATGTGGATGTTCTGATTGAGGATGGAGTAATCTTCAACTTGCTAGGCAGCAACAAAGCAGTGGTAAACATGATTAGTTCCTTGTGTGAGCAGATTAAGGAGGATCACTCCTGCTATAGTGAGATCTGTCAAGACCTTAATAGTCATTATAAGACAGTTTGGCATTCGAGTTGCTTTAATTACGCTCCGTGCATGGAAGCCGTACCTGATCATCTATCAGCTAGCTCCACACTGCTGATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

442

Amino Acids

51.22

Weight (kDa)

5.91

Isoelectric Point (pI)

52.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 24 - 419 7.4e-83 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 890, 1236
Acc65I GGTACC 1 cut(s) 293
AccB1I GGYRCC 1 cut(s) 293
AciI CCGC 1 cut(s) 94
AclWI GGATC 2 cut(s) 267, 1197
AcoI YGGCCR 1 cut(s) 526
AcuI CTGAAG 1 cut(s) 863
AcyI GRCGYC 1 cut(s) 339
AfaI GTAC 2 cut(s) 295, 1290
AluBI AGCT 9 cut(s) 91, 241, 249, 469, 493, 841, 949, 1307, 1311
AluI AGCT 9 cut(s) 91, 241, 249, 469, 493, 841, 949, 1307, 1311
Alw21I GWGCWC 1 cut(s) 397
Alw26I GTCTC 1 cut(s) 537
Alw44I GTGCAC 1 cut(s) 393
AlwI GGATC 2 cut(s) 267, 1197
AoxI GGCC 5 cut(s) 140, 180, 526, 611, 1002
ApaLI GTGCAC 1 cut(s) 393
ApeKI GCWGC 3 cut(s) 110, 594, 1134
AseI ATTAAT 1 cut(s) 300
Asp700I GAANNNNTTC 3 cut(s) 273, 651, 740
Asp718I GGTACC 1 cut(s) 293
AspS9I GGNCC 4 cut(s) 140, 181, 445, 612
AsuC2I CCSGG 1 cut(s) 615
AsuHPI GGTGA 2 cut(s) 408, 629
AsuII TTCGAA 1 cut(s) 991
AsuNHI GCTAGC 1 cut(s) 1307
AvaII GGWCC 1 cut(s) 445
BaeGI GKGCMC 1 cut(s) 397
BaeI ACNNNNGTAYC 2 cut(s) 1004, 1037
BalI TGGCCA 1 cut(s) 528
BanI GGYRCC 1 cut(s) 293
BbsI GAAGAC 1 cut(s) 903
Bbv12I GWGCWC 1 cut(s) 397
BbvCI CCTCAGC 1 cut(s) 425
BbvI GCAGC 3 cut(s) 122, 581, 1146
BccI CCATC 4 cut(s) 250, 537, 644, 1103
BceAI ACGGC 1 cut(s) 1271
BciVI GTATCC 1 cut(s) 1032
BclI TGATCA 2 cut(s) 805, 1294
BcnI CCSGG 1 cut(s) 615
BcoDI GTCTC 1 cut(s) 537
BfaI CTAG 3 cut(s) 332, 1130, 1308
BfmI CTRYAG 2 cut(s) 73, 1201
BfuI GTATCC 1 cut(s) 1032
BglI GCCNNNNNGGC 1 cut(s) 534
BglII AGATCT 1 cut(s) 1209
BisI GCNGC 3 cut(s) 111, 595, 1135
BlsI GCNGC 3 cut(s) 112, 596, 1136
Bme1390I CCNGG 1 cut(s) 615
Bme18I GGWCC 1 cut(s) 445
BmgT120I GGNCC 4 cut(s) 140, 181, 445, 612
BmiI GGNNCC 3 cut(s) 81, 295, 446
BmrFI CCNGG 1 cut(s) 615
BmsI GCATC 1 cut(s) 971
BmtI GCTAGC 1 cut(s) 1311
BpiI GAAGAC 1 cut(s) 903
BplI GAGNNNNNCTC 2 cut(s) 1178, 1210
Bpu10I CCTNAGC 2 cut(s) 242, 425
Bpu14I TTCGAA 1 cut(s) 991
BpuMI CCSGG 1 cut(s) 615
BsaHI GRCGYC 1 cut(s) 339
BsaI GGTCTC 1 cut(s) 537
BsaJI CCNNGG 4 cut(s) 151, 419, 999, 1005
Bse118I RCCGGY 1 cut(s) 290
Bse3DI GCAATG 3 cut(s) 103, 544, 596
BseDI CCNNGG 4 cut(s) 151, 419, 999, 1005
BseGI GGATG 6 cut(s) 241, 327, 636, 986, 1099, 1114
BseMI GCAATG 3 cut(s) 103, 544, 596
BseMII CTCAG 4 cut(s) 158, 401, 416, 933
BseRI GAGGAG 1 cut(s) 111
BseSI GKGCMC 1 cut(s) 397
BseXI GCAGC 3 cut(s) 122, 581, 1146
BseYI CCCAGC 1 cut(s) 83
BshFI GGCC 5 cut(s) 142, 182, 528, 613, 1004
BshNI GGYRCC 1 cut(s) 293
BsiHKAI GWGCWC 1 cut(s) 397
BsiSI CCGG 3 cut(s) 291, 610, 615
BslFI GGGAC 2 cut(s) 185, 458
BsmAI GTCTC 1 cut(s) 537
BsmFI GGGAC 2 cut(s) 185, 458
BsmI GAATGC 2 cut(s) 43, 1249
BsnI GGCC 5 cut(s) 142, 182, 528, 613, 1004
Bso31I GGTCTC 1 cut(s) 537
Bsp119I TTCGAA 1 cut(s) 991
Bsp1286I GDGCHC 1 cut(s) 397
Bsp143I GATC 6 cut(s) 259, 413, 805, 1189, 1209, 1294
Bsp19I CCATGG 2 cut(s) 151, 999
BspACI CCGC 1 cut(s) 94
BspANI GGCC 5 cut(s) 142, 182, 528, 613, 1004
BspCNI CTCAG 4 cut(s) 159, 402, 417, 932
BspHI TCATGA 1 cut(s) 252
BspLI GGNNCC 3 cut(s) 81, 295, 446
BspOI GCTAGC 1 cut(s) 1311
BspPI GGATC 2 cut(s) 267, 1197
BspT104I TTCGAA 1 cut(s) 991
BspT107I GGYRCC 1 cut(s) 293
BspTNI GGTCTC 1 cut(s) 537
BsrDI GCAATG 3 cut(s) 103, 544, 596
BsrFI RCCGGY 1 cut(s) 290
BssAI RCCGGY 1 cut(s) 290
BssECI CCNNGG 4 cut(s) 151, 419, 999, 1005
BssMI GATC 6 cut(s) 259, 413, 805, 1189, 1209, 1294
BssNI GRCGYC 1 cut(s) 339
BssT1I CCWWGG 3 cut(s) 151, 999, 1005
Bst4CI ACNGT 8 cut(s) 16, 274, 420, 436, 456, 584, 786, 1243
BstACI GRCGYC 1 cut(s) 339
BstBI TTCGAA 1 cut(s) 991
BstC8I GCNNGC 3 cut(s) 89, 343, 1309
BstDEI CTNAG 7 cut(s) 167, 242, 410, 425, 702, 872, 919
BstDSI CCRYGG 3 cut(s) 151, 419, 999
BstF5I GGATG 6 cut(s) 241, 327, 636, 986, 1099, 1114
BstKTI GATC 6 cut(s) 262, 416, 808, 1192, 1212, 1297
BstMAI GTCTC 1 cut(s) 537
BstMBI GATC 6 cut(s) 259, 413, 805, 1189, 1209, 1294
BstMWI GCNNNNNNNGC 3 cut(s) 93, 534, 603
BstNSI RCATGY 2 cut(s) 563, 927
BstSCI CCNGG 1 cut(s) 613
BstSFI CTRYAG 2 cut(s) 73, 1201
BstSLI GKGCMC 1 cut(s) 397
BstV1I GCAGC 3 cut(s) 122, 581, 1146
BstV2I GAAGAC 1 cut(s) 903
BstX2I RGATCY 1 cut(s) 1209
BstXI CCANNNNNNTGG 1 cut(s) 1006
BstYI RGATCY 1 cut(s) 1209
BsuI GTATCC 1 cut(s) 1032
BsuRI GGCC 5 cut(s) 142, 182, 528, 613, 1004
BtgI CCRYGG 3 cut(s) 151, 419, 999
BtsCI GGATG 6 cut(s) 241, 327, 636, 986, 1099, 1114
BtsI GCAGTG 2 cut(s) 1152, 1316
BtsIMutI CAGTG 3 cut(s) 744, 1152, 1316
Cac8I GCNNGC 3 cut(s) 89, 343, 1309
CciI TCATGA 1 cut(s) 252
Cfr10I RCCGGY 1 cut(s) 290
Cfr13I GGNCC 4 cut(s) 140, 181, 445, 612
CseI GACGC 1 cut(s) 347
Csp6I GTAC 2 cut(s) 294, 1289
CviQI GTAC 2 cut(s) 294, 1289
DdeI CTNAG 7 cut(s) 167, 242, 410, 425, 702, 872, 919
DpnI GATC 6 cut(s) 261, 415, 807, 1191, 1211, 1296
DpnII GATC 6 cut(s) 259, 413, 805, 1189, 1209, 1294
EaeI YGGCCR 1 cut(s) 526
Eco130I CCWWGG 3 cut(s) 151, 999, 1005
Eco31I GGTCTC 1 cut(s) 537
Eco47I GGWCC 1 cut(s) 445
Eco57I CTGAAG 1 cut(s) 863
EcoO109I RGGNCCY 2 cut(s) 140, 445
EcoT14I CCWWGG 3 cut(s) 151, 999, 1005
EcoT22I ATGCAT 1 cut(s) 986
ErhI CCWWGG 3 cut(s) 151, 999, 1005
FaqI GGGAC 2 cut(s) 185, 458
FbaI TGATCA 2 cut(s) 805, 1294
Fnu4HI GCNGC 3 cut(s) 111, 595, 1135
FokI GGATG 6 cut(s) 248, 314, 623, 993, 1106, 1121
Fsp4HI GCNGC 3 cut(s) 111, 595, 1135
FspBI CTAG 3 cut(s) 332, 1130, 1308
GluI GCNGC 3 cut(s) 111, 595, 1135
GsaI CCCAGC 1 cut(s) 87
HaeIII GGCC 5 cut(s) 142, 182, 528, 613, 1004
HapII CCGG 3 cut(s) 291, 610, 615
HgaI GACGC 1 cut(s) 347
Hin1I GRCGYC 1 cut(s) 339
HincII GTYRAC 2 cut(s) 337, 909
HindII GTYRAC 2 cut(s) 337, 909
HindIII AAGCTT 1 cut(s) 947
HinfI GANTC 1 cut(s) 1063
HpaII CCGG 3 cut(s) 291, 610, 615
HphI GGTGA 2 cut(s) 408, 629
Hpy166II GTNNAC 4 cut(s) 337, 395, 909, 1153
Hpy188I TCNGA 4 cut(s) 376, 646, 1081, 1101
Hpy188III TCNNGA 7 cut(s) 253, 266, 626, 668, 718, 1025, 1217
Hpy8I GTNNAC 4 cut(s) 337, 395, 909, 1153
HpyAV CCTTC 1 cut(s) 838
HpyCH4III ACNGT 8 cut(s) 16, 274, 420, 436, 456, 584, 786, 1243
HpyCH4IV ACGT 2 cut(s) 217, 911
HpyCH4V TGCA 8 cut(s) 41, 213, 395, 833, 851, 984, 1044, 1278
HpyF10VI GCNNNNNNNGC 3 cut(s) 93, 534, 603
HpyF3I CTNAG 7 cut(s) 167, 242, 410, 425, 702, 872, 919
HpySE526I ACGT 2 cut(s) 217, 911
Hsp92I GRCGYC 1 cut(s) 339
KpnI GGTACC 1 cut(s) 297
Ksp22I TGATCA 2 cut(s) 805, 1294
Kzo9I GATC 6 cut(s) 259, 413, 805, 1189, 1209, 1294
LmnI GCTCC 4 cut(s) 96, 246, 1276, 1316
Lsp1109I GCAGC 3 cut(s) 122, 581, 1146
LweI GCATC 1 cut(s) 971
MaeI CTAG 3 cut(s) 332, 1130, 1308
MaeII ACGT 2 cut(s) 217, 911
MaeIII GTNAC 1 cut(s) 305
MalI GATC 6 cut(s) 261, 415, 807, 1191, 1211, 1296
MboI GATC 6 cut(s) 259, 413, 805, 1189, 1209, 1294
MboII GAAGA 5 cut(s) 473, 476, 908, 1097, 1111
MflI RGATCY 1 cut(s) 1209
MhlI GDGCHC 1 cut(s) 397
MlsI TGGCCA 1 cut(s) 528
MluCI AATT 7 cut(s) 280, 301, 351, 370, 799, 819, 1264
MluNI TGGCCA 1 cut(s) 528
MmeI TCCRAC 1 cut(s) 438
MnlI CCTC 9 cut(s) 132, 162, 226, 420, 435, 967, 1075, 1099, 1180
Mox20I TGGCCA 1 cut(s) 528
Mph1103I ATGCAT 1 cut(s) 986
MroXI GAANNNNTTC 3 cut(s) 273, 651, 740
MscI TGGCCA 1 cut(s) 528
MseI TTAA 8 cut(s) 300, 690, 822, 915, 1074, 1182, 1224, 1263
Msp20I TGGCCA 1 cut(s) 528
MspI CCGG 3 cut(s) 291, 610, 615
MspR9I CCNGG 1 cut(s) 615
Mva1269I GAATGC 2 cut(s) 43, 1249
MwoI GCNNNNNNNGC 3 cut(s) 93, 534, 603
NciI CCSGG 1 cut(s) 615
NcoI CCATGG 2 cut(s) 151, 999
NdeII GATC 6 cut(s) 259, 413, 805, 1189, 1209, 1294
NheI GCTAGC 1 cut(s) 1307
NlaIV GGNNCC 3 cut(s) 81, 295, 446
NmuCI GTSAC 1 cut(s) 305
NsiI ATGCAT 1 cut(s) 986
NspI RCATGY 2 cut(s) 563, 927
NspV TTCGAA 1 cut(s) 991
PagI TCATGA 1 cut(s) 252
PctI GAATGC 2 cut(s) 43, 1249
PdmI GAANNNNTTC 3 cut(s) 273, 651, 740
PfeI GAWTC 1 cut(s) 1063
PkrI GCNGC 3 cut(s) 112, 596, 1136
PpuMI RGGWCCY 1 cut(s) 445
PshBI ATTAAT 1 cut(s) 300
PsiI TTATAA 2 cut(s) 890, 1236
Psp5II RGGWCCY 1 cut(s) 445
PspFI CCCAGC 1 cut(s) 83
PspN4I GGNNCC 3 cut(s) 81, 295, 446
PspPI GGNCC 4 cut(s) 140, 181, 445, 612
PspPPI RGGWCCY 1 cut(s) 445
PsuI RGATCY 1 cut(s) 1209
RsaI GTAC 2 cut(s) 295, 1290
RsaNI GTAC 2 cut(s) 294, 1289
SaqAI TTAA 8 cut(s) 300, 690, 822, 915, 1074, 1182, 1224, 1263
SatI GCNGC 3 cut(s) 111, 595, 1135
Sau3AI GATC 6 cut(s) 259, 413, 805, 1189, 1209, 1294
Sau96I GGNCC 4 cut(s) 140, 181, 445, 612
ScrFI CCNGG 1 cut(s) 615
SduI GDGCHC 1 cut(s) 397
SfaNI GCATC 1 cut(s) 971
SfcI CTRYAG 2 cut(s) 73, 1201
SfuI TTCGAA 1 cut(s) 991
SinI GGWCC 1 cut(s) 445
Sse9I AATT 7 cut(s) 280, 301, 351, 370, 799, 819, 1264
SsiI CCGC 1 cut(s) 94
SspI AATATT 1 cut(s) 197
SspMI CTAG 3 cut(s) 332, 1130, 1308
StyD4I CCNGG 1 cut(s) 613
StyI CCWWGG 3 cut(s) 151, 999, 1005
TaaI ACNGT 8 cut(s) 16, 274, 420, 436, 456, 584, 786, 1243
TaiI ACGT 2 cut(s) 220, 914
TaqI TCGA 4 cut(s) 265, 312, 991, 1253
TasI AATT 7 cut(s) 280, 301, 351, 370, 799, 819, 1264
TfiI GAWTC 1 cut(s) 1063
Tru1I TTAA 8 cut(s) 300, 690, 822, 915, 1074, 1182, 1224, 1263
Tru9I TTAA 8 cut(s) 300, 690, 822, 915, 1074, 1182, 1224, 1263
TscAI CASTG 3 cut(s) 751, 1152, 1323
TseFI GTSAC 1 cut(s) 305
TseI GCWGC 3 cut(s) 110, 594, 1134
Tsp45I GTSAC 1 cut(s) 305
TspDTI ATGAA 4 cut(s) 120, 241, 337, 1076
TspGWI ACGGA 1 cut(s) 1263
TspRI CASTG 3 cut(s) 751, 1152, 1323
VneI GTGCAC 1 cut(s) 393
VpaK11BI GGWCC 1 cut(s) 445
VspI ATTAAT 1 cut(s) 300
XceI RCATGY 2 cut(s) 563, 927
XcmI CCANNNNNNNNNTGG 1 cut(s) 536
XmnI GAANNNNTTC 3 cut(s) 273, 651, 740
XspI CTAG 3 cut(s) 332, 1130, 1308
Zsp2I ATGCAT 1 cut(s) 986
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.