pycom02g18340

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0000821
Physical Location & Seq
Reverse (-)
16574589 .. 16576258
1670 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g18340.1

Sequence Viewer

Length: 1407 bp
ATGAAATATCGACGGAAGAACCTAAGAAGGATAAAGACAACATCACAAATCGGGGAGAAAAATGATTGCGAACAAGATTTCCATGACCACGTACTAGTCTTGGACCAGCCAAACAAGTGGTGTATCTACAGGGTTCCCAGCAAACTTCGCAAAGTAAACGAAGCAGCATACACTCCTCAGTTACTATCAATCGGCCTTTTCCACTACGGCAAGCCGGAACTCAAGGACATGGAGACCCACAAAAATATGTACTACAAGAATTTTTCCGCGCGTTGTTCGAAGAAGGACTTTGAACTAAAAAAATTCATCAGGGCTCGTCAAGAACACATTCTTCGTTGTTATGCAGGGGCCATTGAGCTTGAAGAAGATCCTGTTGACATAATTGTGGTTGATGCCTGCTTCATCATTGAGCTCTTTTTGATGAACTCCTCTGAAACAAACAATCATGAAAACGATTACATTTTAAGATCACCGTGGATGAGGAAAGTTGTAGAGCAGGAACTAATATTGTTTGAAAACCAGCTTTCGTATTCTCTTCTTCAAGAGCTATATGACTTTGCGGTGCCAGCCTCCAGTTCCCCTCATTCCAAAGAACTCTCAGGTGATCATTGTATTGATGTTTCAGAAGCTGAACCTGTCTTTGATAGTACAGGTGTTCCAGAAGCTGAACCTGTCCCTGATCATCCCTTTCTCAAGCTTACCTGCGAGTTCTTCAAAGCTTACAGCAAGGGAAAATCCGTCAAGAATGGGTTAAAACCAAAACACTTCACTGATTTGGTAAGACATTTTCTCTGTCCCGAAGAAGAAATGACCTTTGTACATAATGCTTGTCCTACAAAAAATATATATGCTGTAAGGAAGCTGAAGGCAGCAGGGGTGAAGTTTACGCCACTTCATGAAGACGGATACTTTATCATAAAAGGAGACAAGCCTACTAAATGCAAGTTTAACTTGGCGTGTTTTCGAAATATGAACTTAAAGCTTGCACAATTTTGTGTCAAGGATGAGATAGAGTTCGTTATTCGAAACATCATGGCCTTGGAGCAGTTTTTGTACCCGGACAAGCCTTATATCTGCAATTACTTTTTGCTTATGGATCAACTTGTGGACACTGTAGAAGATGTTGATTTGCTGGTTGAGAACAAAGTTATTGTTAACATGCTGGGCAGCAACGAAGCAGTGGCAAAGCTGGTTAATAGACTTTGTGAGCAGATTATGGAGGATAAATCTTGCTATGCTGACGTCTGTAAACAGCTTAACAAGCACTATGAGAATTTCTGGAACCATCATGTGGCGACCCTGAAACGAGTTTACTTCAAGGATCTTTGGACAAGCAGTTCAACTATACTGGGACTTTTCGTTCTGGTTTTCTCCATCATTGGAAGCATAAAGTCGCTCAAATCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

469

Amino Acids

54.4

Weight (kDa)

6.96

Isoelectric Point (pI)

44.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 42 - 457 1.5e-90 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1245
Acc36I ACCTGC 1 cut(s) 710
AccB1I GGYRCC 1 cut(s) 562
AccB7I CCANNNNNTGG 1 cut(s) 1291
AccII CGCG 2 cut(s) 269, 271
AciI CCGC 2 cut(s) 267, 560
AclWI GGATC 3 cut(s) 362, 1104, 1329
AcsI RAATTY 3 cut(s) 259, 302, 1273
AcuI CTGAAG 1 cut(s) 884
AcyI GRCGYC 1 cut(s) 1242
AfaI GTAC 5 cut(s) 93, 251, 649, 819, 1055
AfiI CCNNNNNNNGG 1 cut(s) 1291
AgsI TTSAA 7 cut(s) 293, 362, 515, 542, 715, 1318, 1341
AhlI ACTAGT 1 cut(s) 94
AloI GAACNNNNNNTCC 6 cut(s) 63, 95, 1321, 1344, 1353, 1376
Alw21I GWGCWC 1 cut(s) 414
Alw26I GTCTC 2 cut(s) 227, 918
AlwI GGATC 3 cut(s) 362, 1104, 1329
AlwNI CAGNNNCTG 2 cut(s) 629, 665
AoxI GGCC 3 cut(s) 193, 348, 1035
ApeKI GCWGC 3 cut(s) 164, 869, 1167
ApoI RAATTY 3 cut(s) 259, 302, 1273
Asp700I GAANNNNTTC 1 cut(s) 327
AspLEI GCGC 1 cut(s) 271
AspS9I GGNCC 2 cut(s) 103, 348
AsuC2I CCSGG 1 cut(s) 1058
AsuHPI GGTGA 3 cut(s) 462, 614, 889
AsuII TTCGAA 3 cut(s) 278, 964, 1024
AvaII GGWCC 1 cut(s) 103
BanI GGYRCC 1 cut(s) 562
BanII GRGCYC 2 cut(s) 316, 414
BbsI GAAGAC 1 cut(s) 906
Bbv12I GWGCWC 1 cut(s) 414
BbvI GCAGC 3 cut(s) 176, 881, 1179
BccI CCATC 2 cut(s) 1293, 1382
BceAI ACGGC 1 cut(s) 223
BciVI GTATCC 1 cut(s) 899
BclI TGATCA 2 cut(s) 604, 679
BcnI CCSGG 1 cut(s) 1058
BcoDI GTCTC 2 cut(s) 227, 918
BcuI ACTAGT 1 cut(s) 94
BfaI CTAG 1 cut(s) 95
BfmI CTRYAG 2 cut(s) 127, 1113
BfuAI ACCTGC 1 cut(s) 710
BfuI GTATCC 1 cut(s) 899
BisI GCNGC 3 cut(s) 165, 870, 1168
BlsI GCNGC 3 cut(s) 166, 871, 1169
Bme1390I CCNGG 1 cut(s) 1058
Bme18I GGWCC 1 cut(s) 103
BmgT120I GGNCC 2 cut(s) 103, 348
BmiI GGNNCC 4 cut(s) 135, 349, 564, 1283
BmrFI CCNGG 1 cut(s) 1058
BmrI ACTGGG 1 cut(s) 1358
BmsI GCATC 1 cut(s) 382
BmuI ACTGGG 1 cut(s) 1358
BpiI GAAGAC 1 cut(s) 906
BpmI CTGGAG 1 cut(s) 556
Bpu14I TTCGAA 3 cut(s) 278, 964, 1024
BpuEI CTTGAG 2 cut(s) 206, 677
BpuMI CCSGG 1 cut(s) 1058
BsaAI YACGTR 1 cut(s) 91
BsaHI GRCGYC 1 cut(s) 1242
BsaI GGTCTC 1 cut(s) 227
BsaJI CCNNGG 2 cut(s) 473, 1038
Bsc4I CCNNNNNNNGG 1 cut(s) 1291
Bse1I ACTGG 2 cut(s) 573, 1353
BseDI CCNNGG 2 cut(s) 473, 1038
BseGI GGATG 3 cut(s) 483, 682, 1009
BseLI CCNNNNNNNGG 1 cut(s) 1291
BseMII CTCAG 2 cut(s) 191, 612
BseNI ACTGG 2 cut(s) 573, 1353
BseRI GAGGAG 2 cut(s) 165, 418
BseXI GCAGC 3 cut(s) 176, 881, 1179
BseYI CCCAGC 2 cut(s) 137, 1162
Bsh1236I CGCG 2 cut(s) 269, 271
BshFI GGCC 3 cut(s) 195, 350, 1037
BshNI GGYRCC 1 cut(s) 562
BsiHKAI GWGCWC 1 cut(s) 414
BsiSI CCGG 2 cut(s) 215, 1058
BslFI GGGAC 3 cut(s) 659, 780, 1365
BslI CCNNNNNNNGG 1 cut(s) 1291
BsmAI GTCTC 2 cut(s) 227, 918
BsmFI GGGAC 3 cut(s) 659, 780, 1365
BsnI GGCC 3 cut(s) 195, 350, 1037
Bso31I GGTCTC 1 cut(s) 227
Bsp119I TTCGAA 3 cut(s) 278, 964, 1024
Bsp1286I GDGCHC 2 cut(s) 316, 414
Bsp1407I TGTACA 1 cut(s) 817
Bsp143I GATC 6 cut(s) 367, 467, 604, 679, 1096, 1321
BspACI CCGC 2 cut(s) 267, 560
BspANI GGCC 3 cut(s) 195, 350, 1037
BspCNI CTCAG 2 cut(s) 190, 611
BspFNI CGCG 2 cut(s) 269, 271
BspHI TCATGA 2 cut(s) 445, 895
BspLI GGNNCC 4 cut(s) 135, 349, 564, 1283
BspMI ACCTGC 1 cut(s) 710
BspPI GGATC 3 cut(s) 362, 1104, 1329
BspT104I TTCGAA 3 cut(s) 278, 964, 1024
BspT107I GGYRCC 1 cut(s) 562
BspTNI GGTCTC 1 cut(s) 227
BsrGI TGTACA 1 cut(s) 817
BsrI ACTGG 2 cut(s) 573, 1353
BssECI CCNNGG 2 cut(s) 473, 1038
BssMI GATC 6 cut(s) 367, 467, 604, 679, 1096, 1321
BssNI GRCGYC 1 cut(s) 1242
BssT1I CCWWGG 1 cut(s) 1038
Bst4CI ACNGT 2 cut(s) 474, 1114
Bst6I CTCTTC 1 cut(s) 540
BstACI GRCGYC 1 cut(s) 1242
BstAUI TGTACA 1 cut(s) 817
BstBAI YACGTR 1 cut(s) 91
BstBI TTCGAA 3 cut(s) 278, 964, 1024
BstC8I GCNNGC 4 cut(s) 212, 397, 567, 984
BstDEI CTNAG 3 cut(s) 23, 177, 598
BstDSI CCRYGG 1 cut(s) 473
BstF5I GGATG 3 cut(s) 483, 682, 1009
BstFNI CGCG 2 cut(s) 269, 271
BstHHI GCGC 1 cut(s) 271
BstKTI GATC 6 cut(s) 370, 470, 607, 682, 1099, 1324
BstMAI GTCTC 2 cut(s) 227, 918
BstMBI GATC 6 cut(s) 367, 467, 604, 679, 1096, 1321
BstMWI GCNNNNNNNGC 3 cut(s) 147, 566, 1261
BstNSI RCATGY 1 cut(s) 1162
BstSCI CCNGG 1 cut(s) 1056
BstSFI CTRYAG 2 cut(s) 127, 1113
BstUI CGCG 2 cut(s) 269, 271
BstV1I GCAGC 3 cut(s) 176, 881, 1179
BstV2I GAAGAC 1 cut(s) 906
BstX2I RGATCY 2 cut(s) 367, 1321
BstXI CCANNNNNNTGG 1 cut(s) 117
BstYI RGATCY 2 cut(s) 367, 1321
BsuI GTATCC 1 cut(s) 899
BsuRI GGCC 3 cut(s) 195, 350, 1037
BtgI CCRYGG 1 cut(s) 473
BtsCI GGATG 3 cut(s) 483, 682, 1009
BtsI GCAGTG 1 cut(s) 1185
BtsIMutI CAGTG 3 cut(s) 768, 1110, 1185
BveI ACCTGC 1 cut(s) 710
Cac8I GCNNGC 4 cut(s) 212, 397, 567, 984
CaiI CAGNNNCTG 2 cut(s) 629, 665
CciI TCATGA 2 cut(s) 445, 895
CfoI GCGC 1 cut(s) 271
Cfr13I GGNCC 2 cut(s) 103, 348
Csp6I GTAC 5 cut(s) 92, 250, 648, 818, 1054
CviAII CATG 7 cut(s) 83, 229, 446, 896, 1033, 1159, 1289
CviQI GTAC 5 cut(s) 92, 250, 648, 818, 1054
DdeI CTNAG 3 cut(s) 23, 177, 598
DpnI GATC 6 cut(s) 369, 469, 606, 681, 1098, 1323
DpnII GATC 6 cut(s) 367, 467, 604, 679, 1096, 1321
Eam1104I CTCTTC 1 cut(s) 540
EarI CTCTTC 1 cut(s) 540
Ecl136II GAGCTC 1 cut(s) 412
Eco130I CCWWGG 1 cut(s) 1038
Eco24I GRGCYC 2 cut(s) 316, 414
Eco31I GGTCTC 1 cut(s) 227
Eco47I GGWCC 1 cut(s) 103
Eco53kI GAGCTC 1 cut(s) 412
Eco57I CTGAAG 1 cut(s) 884
EcoICRI GAGCTC 1 cut(s) 412
EcoT14I CCWWGG 1 cut(s) 1038
EcoT38I GRGCYC 2 cut(s) 316, 414
ErhI CCWWGG 1 cut(s) 1038
FaeI CATG 7 cut(s) 86, 232, 449, 899, 1036, 1162, 1292
FalI AAGNNNNNCTT 4 cut(s) 272, 304, 935, 967
FaqI GGGAC 3 cut(s) 659, 780, 1365
FatI CATG 7 cut(s) 82, 228, 445, 895, 1032, 1158, 1288
FbaI TGATCA 2 cut(s) 604, 679
Fnu4HI GCNGC 3 cut(s) 165, 870, 1168
FokI GGATG 3 cut(s) 490, 669, 1016
FriOI GRGCYC 2 cut(s) 316, 414
Fsp4HI GCNGC 3 cut(s) 165, 870, 1168
FspBI CTAG 1 cut(s) 95
GlaI GCGC 1 cut(s) 270
GluI GCNGC 3 cut(s) 165, 870, 1168
GsaI CCCAGC 2 cut(s) 141, 1166
GsuI CTGGAG 1 cut(s) 556
HaeIII GGCC 3 cut(s) 195, 350, 1037
HapII CCGG 2 cut(s) 215, 1058
HhaI GCGC 1 cut(s) 271
Hin1I GRCGYC 1 cut(s) 1242
Hin1II CATG 7 cut(s) 86, 232, 449, 899, 1036, 1162, 1292
Hin6I GCGC 1 cut(s) 269
HinP1I GCGC 1 cut(s) 269
HincII GTYRAC 2 cut(s) 376, 1156
HindII GTYRAC 2 cut(s) 376, 1156
HindIII AAGCTT 3 cut(s) 695, 717, 980
HpaI GTTAAC 1 cut(s) 1156
HpaII CCGG 2 cut(s) 215, 1058
HphI GGTGA 3 cut(s) 462, 614, 889
Hpy166II GTNNAC 7 cut(s) 157, 376, 885, 1108, 1156, 1250, 1312
Hpy188I TCNGA 2 cut(s) 433, 625
Hpy188III TCNNGA 8 cut(s) 320, 446, 542, 659, 742, 797, 896, 1279
Hpy8I GTNNAC 7 cut(s) 157, 376, 885, 1108, 1156, 1250, 1312
Hpy99I CGWCG 1 cut(s) 15
HpyAV CCTTC 3 cut(s) 21, 277, 859
HpyCH4III ACNGT 2 cut(s) 474, 1114
HpyCH4IV ACGT 2 cut(s) 90, 1242
HpyCH4V TGCA 4 cut(s) 344, 942, 986, 1077
HpyF10VI GCNNNNNNNGC 3 cut(s) 147, 566, 1261
HpyF3I CTNAG 3 cut(s) 23, 177, 598
HpySE526I ACGT 2 cut(s) 90, 1242
Hsp92I GRCGYC 1 cut(s) 1242
Hsp92II CATG 7 cut(s) 86, 232, 449, 899, 1036, 1162, 1292
HspAI GCGC 1 cut(s) 269
Ksp22I TGATCA 2 cut(s) 604, 679
KspAI GTTAAC 1 cut(s) 1156
Kzo9I GATC 6 cut(s) 367, 467, 604, 679, 1096, 1321
LmnI GCTCC 1 cut(s) 1042
Lsp1109I GCAGC 3 cut(s) 176, 881, 1179
LweI GCATC 1 cut(s) 382
MaeI CTAG 1 cut(s) 95
MaeII ACGT 2 cut(s) 90, 1242
MaeIII GTNAC 1 cut(s) 180
MalI GATC 6 cut(s) 369, 469, 606, 681, 1098, 1323
MboI GATC 6 cut(s) 367, 467, 604, 679, 1096, 1321
MflI RGATCY 2 cut(s) 367, 1321
MhlI GDGCHC 2 cut(s) 316, 414
MluCI AATT 6 cut(s) 259, 302, 381, 989, 1078, 1273
MnlI CCTC 6 cut(s) 186, 439, 474, 580, 591, 1213
MroXI GAANNNNTTC 1 cut(s) 327
MseI TTAA 7 cut(s) 464, 752, 948, 977, 1155, 1194, 1257
MslI CAYNNNNRTG 1 cut(s) 383
MspI CCGG 2 cut(s) 215, 1058
MspR9I CCNGG 1 cut(s) 1058
MvnI CGCG 2 cut(s) 269, 271
MwoI GCNNNNNNNGC 3 cut(s) 147, 566, 1261
NciI CCSGG 1 cut(s) 1058
NdeII GATC 6 cut(s) 367, 467, 604, 679, 1096, 1321
NlaIII CATG 7 cut(s) 86, 232, 449, 899, 1036, 1162, 1292
NlaIV GGNNCC 4 cut(s) 135, 349, 564, 1283
NspI RCATGY 1 cut(s) 1162
NspV TTCGAA 3 cut(s) 278, 964, 1024
PagI TCATGA 2 cut(s) 445, 895
PdmI GAANNNNTTC 1 cut(s) 327
PflMI CCANNNNNTGG 1 cut(s) 1291
PkrI GCNGC 3 cut(s) 166, 871, 1169
Ppu21I YACGTR 1 cut(s) 91
Psp124BI GAGCTC 1 cut(s) 414
PspFI CCCAGC 2 cut(s) 137, 1162
PspN4I GGNNCC 4 cut(s) 135, 349, 564, 1283
PspPI GGNCC 2 cut(s) 103, 348
PstNI CAGNNNCTG 2 cut(s) 629, 665
PsuI RGATCY 2 cut(s) 367, 1321
RsaI GTAC 5 cut(s) 93, 251, 649, 819, 1055
RsaNI GTAC 5 cut(s) 92, 250, 648, 818, 1054
RseI CAYNNNNRTG 1 cut(s) 383
SacI GAGCTC 1 cut(s) 414
SaqAI TTAA 7 cut(s) 464, 752, 948, 977, 1155, 1194, 1257
SatI GCNGC 3 cut(s) 165, 870, 1168
Sau3AI GATC 6 cut(s) 367, 467, 604, 679, 1096, 1321
Sau96I GGNCC 2 cut(s) 103, 348
ScrFI CCNGG 1 cut(s) 1058
SduI GDGCHC 2 cut(s) 316, 414
SfaNI GCATC 1 cut(s) 382
SfcI CTRYAG 2 cut(s) 127, 1113
SfuI TTCGAA 3 cut(s) 278, 964, 1024
SinI GGWCC 1 cut(s) 103
SmiMI CAYNNNNRTG 1 cut(s) 383
SmlI CTYRAG 2 cut(s) 221, 692
SmoI CTYRAG 2 cut(s) 221, 692
SpeI ACTAGT 1 cut(s) 94
Sse9I AATT 6 cut(s) 259, 302, 381, 989, 1078, 1273
SsiI CCGC 2 cut(s) 267, 560
SspI AATATT 1 cut(s) 507
SspMI CTAG 1 cut(s) 95
SstI GAGCTC 1 cut(s) 414
StyD4I CCNGG 1 cut(s) 1056
StyI CCWWGG 1 cut(s) 1038
TaaI ACNGT 2 cut(s) 474, 1114
TaiI ACGT 2 cut(s) 93, 1245
TaqI TCGA 4 cut(s) 10, 278, 964, 1024
TasI AATT 6 cut(s) 259, 302, 381, 989, 1078, 1273
TatI WGTACW 3 cut(s) 249, 647, 817
Tru1I TTAA 7 cut(s) 464, 752, 948, 977, 1155, 1194, 1257
Tru9I TTAA 7 cut(s) 464, 752, 948, 977, 1155, 1194, 1257
TscAI CASTG 3 cut(s) 775, 1117, 1185
TseI GCWGC 3 cut(s) 164, 869, 1167
TspDTI ATGAA 8 cut(s) 17, 295, 391, 437, 462, 884, 912, 986
TspGWI ACGGA 3 cut(s) 28, 727, 918
TspRI CASTG 3 cut(s) 775, 1117, 1185
Van91I CCANNNNNTGG 1 cut(s) 1291
VpaK11BI GGWCC 1 cut(s) 103
XapI RAATTY 3 cut(s) 259, 302, 1273
XceI RCATGY 1 cut(s) 1162
XmnI GAANNNNTTC 1 cut(s) 327
XspI CTAG 1 cut(s) 95
ZraI GACGTC 1 cut(s) 1243
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.