Rh4DG017100

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0000821
Physical Location & Seq
Reverse (-)
2606075 .. 2610327
4253 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG017100.1

Sequence Viewer

Length: 1377 bp
ATGGTAGAACCAACTGTTCAAAGCGAACAAATAGAGAATGCACCCGTATTGTTCTCGCAGGAATGGTGTATCTATAGGGTTCCCAGCCAGCTCCGCAATGTAGATGAAGCAGCATACACTCCTCAAACACTTTCAATAGGGCCTTTCCACCATGGAAATCCAAAACTGAGGGACATGGTGGCCCATAAGAACAAATATTATGAGTTGTTTTGCAGACGTTCTGGGAAAAGTGAGGATGAGCTAAGGAGCTTCATGATGGATCATCGAGAAACAGTTCTAAATTGTTATGCCGGTACCATTAATTGTGACATCGACTTTTTGAAACTCATCCTAGTTGACGCCTGCTTCATAATTGAACTCTTTTTGACTAATTCTGAACAAGAACAACATTGTGCACATTATTATATACTGAGATCACCGTGGCTGAGGAAAACGGTGGAGAGGGACCTGATACTGTTGGAAAATCAGCTTCCTTATTTTCTTCTTCAAAAGCTATATGACTTTGCCATACCTACCTTTTCCAATGGCCATCTTGGCAATGGAGACCAAGCACAAGGACATGCTCATAAAGATAGCACCAACAGTCAGCAATGCTGCTTCCCTTGCTTCCGGCCCGGTGATTTTCAAGAGAACCCCATCCAATCTGAACCTATTCAACATGGCTTTCTTGAACTTACCTGTAAGTTTTTTAAGGCATACACTAAGGGAAAATCTGTCAGGAATGGATTTGAGCCGAAACACTTCACTGATTTGGTTAGACACTTTCTATGTCCATACAAGGAACTGTTGTGGGTAAAAAATTATGATCAGCCCGTCAAAATTAAATACCATGCAACAAAGCTGAAGGTTGCAGGAGTGAAGTTTGTGCCACTTAGAGAACCATTTGTTATAAAGGAAGACCCTGTGTTGACGTTTAACTCAGCATGTTTGACACATAAGGAGTTGAAGCTTACAAGACTTTGGGCAGAGTATGAGGCAGGATGCATTTTTCGAAACACCATGGCCTTGGAACAACTTATGTATCCAGAAAAGCCTTATATTTGCAGTTATTTTTCGCTGATGAATCAACTCATTAACTCTGAGGAAGATGTGGATGTTCTGATTGAGGATGGAGTAATCTTCAACTTGCTAGGCAGCAACAAAGCAGTGGTAAACATGATTAGTTCCTTGTGTGAGCAGATTAAGGAGGATCACTCCTGCTATAGTGAGATCTGTCAAGACCTTAATAGTCATTATAAGACAGTTTGGTACCACCCCTTGGTAATAACGAGACAGGTTTACTTCAAGGATCTTTGGAGAGGCAGTACAGCTATACTAGGATTCGTTGTCCTAATTTTCACAATTCTTCAATCCCTTCAGGCATTCGAGTTGCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

458

Amino Acids

53.31

Weight (kDa)

6.11

Isoelectric Point (pI)

50.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 24 - 445 1e-87 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 890, 1236
Acc65I GGTACC 2 cut(s) 293, 1248
AccB1I GGYRCC 2 cut(s) 293, 1248
AccB7I CCANNNNNTGG 1 cut(s) 1258
AciI CCGC 1 cut(s) 94
AclWI GGATC 3 cut(s) 267, 1197, 1296
AcoI YGGCCR 1 cut(s) 526
AcuI CTGAAG 2 cut(s) 863, 1340
AcyI GRCGYC 1 cut(s) 339
AfaI GTAC 3 cut(s) 295, 1250, 1306
AfiI CCNNNNNNNGG 1 cut(s) 1258
AluBI AGCT 8 cut(s) 91, 241, 249, 469, 493, 841, 949, 1310
AluI AGCT 8 cut(s) 91, 241, 249, 469, 493, 841, 949, 1310
Alw21I GWGCWC 1 cut(s) 397
Alw26I GTCTC 2 cut(s) 537, 1264
Alw44I GTGCAC 1 cut(s) 393
AlwI GGATC 3 cut(s) 267, 1197, 1296
AoxI GGCC 5 cut(s) 140, 180, 526, 611, 1002
ApaLI GTGCAC 1 cut(s) 393
ApeKI GCWGC 3 cut(s) 110, 594, 1134
AseI ATTAAT 1 cut(s) 300
Asp700I GAANNNNTTC 3 cut(s) 273, 651, 740
Asp718I GGTACC 2 cut(s) 293, 1248
AspS9I GGNCC 4 cut(s) 140, 181, 445, 612
AsuC2I CCSGG 1 cut(s) 615
AsuHPI GGTGA 2 cut(s) 408, 629
AsuII TTCGAA 1 cut(s) 991
AvaII GGWCC 1 cut(s) 445
BaeGI GKGCMC 1 cut(s) 397
BaeI ACNNNNGTAYC 2 cut(s) 1004, 1037
BalI TGGCCA 1 cut(s) 528
BanI GGYRCC 2 cut(s) 293, 1248
BbsI GAAGAC 1 cut(s) 903
Bbv12I GWGCWC 1 cut(s) 397
BbvCI CCTCAGC 1 cut(s) 425
BbvI GCAGC 3 cut(s) 122, 581, 1146
BccI CCATC 4 cut(s) 250, 537, 644, 1103
BciVI GTATCC 1 cut(s) 1032
BclI TGATCA 1 cut(s) 805
BcnI CCSGG 1 cut(s) 615
BcoDI GTCTC 2 cut(s) 537, 1264
BfaI CTAG 3 cut(s) 332, 1130, 1316
BfmI CTRYAG 2 cut(s) 73, 1201
BfuI GTATCC 1 cut(s) 1032
BglI GCCNNNNNGGC 1 cut(s) 534
BglII AGATCT 1 cut(s) 1209
BisI GCNGC 3 cut(s) 111, 595, 1135
BlsI GCNGC 3 cut(s) 112, 596, 1136
Bme1390I CCNGG 1 cut(s) 615
Bme18I GGWCC 1 cut(s) 445
BmgT120I GGNCC 4 cut(s) 140, 181, 445, 612
BmiI GGNNCC 4 cut(s) 81, 295, 446, 1250
BmrFI CCNGG 1 cut(s) 615
BmsI GCATC 1 cut(s) 971
BpiI GAAGAC 1 cut(s) 903
BplI GAGNNNNNCTC 2 cut(s) 1178, 1210
Bpu10I CCTNAGC 2 cut(s) 242, 425
Bpu14I TTCGAA 1 cut(s) 991
BpuMI CCSGG 1 cut(s) 615
BsaHI GRCGYC 1 cut(s) 339
BsaI GGTCTC 1 cut(s) 537
BsaJI CCNNGG 5 cut(s) 151, 419, 999, 1005, 1257
BsaXI ACNNNNNCTCC 2 cut(s) 1288, 1318
Bsc4I CCNNNNNNNGG 1 cut(s) 1258
Bse118I RCCGGY 1 cut(s) 290
Bse3DI GCAATG 3 cut(s) 103, 544, 596
BseDI CCNNGG 5 cut(s) 151, 419, 999, 1005, 1257
BseGI GGATG 6 cut(s) 241, 327, 636, 986, 1099, 1114
BseLI CCNNNNNNNGG 1 cut(s) 1258
BseMI GCAATG 3 cut(s) 103, 544, 596
BseMII CTCAG 5 cut(s) 158, 401, 416, 933, 1071
BseRI GAGGAG 1 cut(s) 111
BseSI GKGCMC 1 cut(s) 397
BseXI GCAGC 3 cut(s) 122, 581, 1146
BseYI CCCAGC 1 cut(s) 83
BshFI GGCC 5 cut(s) 142, 182, 528, 613, 1004
BshNI GGYRCC 2 cut(s) 293, 1248
BsiHKAI GWGCWC 1 cut(s) 397
BsiSI CCGG 3 cut(s) 291, 610, 615
BslFI GGGAC 2 cut(s) 185, 458
BslI CCNNNNNNNGG 1 cut(s) 1258
BsmAI GTCTC 2 cut(s) 537, 1264
BsmFI GGGAC 2 cut(s) 185, 458
BsmI GAATGC 2 cut(s) 43, 1361
BsnI GGCC 5 cut(s) 142, 182, 528, 613, 1004
Bso31I GGTCTC 1 cut(s) 537
Bsp119I TTCGAA 1 cut(s) 991
Bsp1286I GDGCHC 1 cut(s) 397
Bsp143I GATC 6 cut(s) 259, 413, 805, 1189, 1209, 1288
Bsp19I CCATGG 2 cut(s) 151, 999
BspACI CCGC 1 cut(s) 94
BspANI GGCC 5 cut(s) 142, 182, 528, 613, 1004
BspCNI CTCAG 5 cut(s) 159, 402, 417, 932, 1072
BspHI TCATGA 1 cut(s) 252
BspLI GGNNCC 4 cut(s) 81, 295, 446, 1250
BspPI GGATC 3 cut(s) 267, 1197, 1296
BspT104I TTCGAA 1 cut(s) 991
BspT107I GGYRCC 2 cut(s) 293, 1248
BspTNI GGTCTC 1 cut(s) 537
BsrDI GCAATG 3 cut(s) 103, 544, 596
BsrFI RCCGGY 1 cut(s) 290
BssAI RCCGGY 1 cut(s) 290
BssECI CCNNGG 5 cut(s) 151, 419, 999, 1005, 1257
BssMI GATC 6 cut(s) 259, 413, 805, 1189, 1209, 1288
BssNI GRCGYC 1 cut(s) 339
BssT1I CCWWGG 4 cut(s) 151, 999, 1005, 1257
Bst4CI ACNGT 8 cut(s) 16, 274, 420, 436, 456, 584, 786, 1243
BstACI GRCGYC 1 cut(s) 339
BstBI TTCGAA 1 cut(s) 991
BstC8I GCNNGC 2 cut(s) 89, 343
BstDEI CTNAG 8 cut(s) 167, 242, 410, 425, 702, 872, 919, 1080
BstDSI CCRYGG 3 cut(s) 151, 419, 999
BstF5I GGATG 6 cut(s) 241, 327, 636, 986, 1099, 1114
BstKTI GATC 6 cut(s) 262, 416, 808, 1192, 1212, 1291
BstMAI GTCTC 2 cut(s) 537, 1264
BstMBI GATC 6 cut(s) 259, 413, 805, 1189, 1209, 1288
BstMWI GCNNNNNNNGC 3 cut(s) 93, 534, 603
BstNSI RCATGY 2 cut(s) 563, 927
BstSCI CCNGG 1 cut(s) 613
BstSFI CTRYAG 2 cut(s) 73, 1201
BstSLI GKGCMC 1 cut(s) 397
BstV1I GCAGC 3 cut(s) 122, 581, 1146
BstV2I GAAGAC 1 cut(s) 903
BstX2I RGATCY 2 cut(s) 1209, 1288
BstXI CCANNNNNNTGG 1 cut(s) 1006
BstYI RGATCY 2 cut(s) 1209, 1288
BsuI GTATCC 1 cut(s) 1032
BsuRI GGCC 5 cut(s) 142, 182, 528, 613, 1004
BtgI CCRYGG 3 cut(s) 151, 419, 999
BtsCI GGATG 6 cut(s) 241, 327, 636, 986, 1099, 1114
BtsI GCAGTG 1 cut(s) 1152
BtsIMutI CAGTG 2 cut(s) 744, 1152
Cac8I GCNNGC 2 cut(s) 89, 343
CciI TCATGA 1 cut(s) 252
Cfr10I RCCGGY 1 cut(s) 290
Cfr13I GGNCC 4 cut(s) 140, 181, 445, 612
CseI GACGC 1 cut(s) 347
Csp6I GTAC 3 cut(s) 294, 1249, 1305
CviAII CATG 9 cut(s) 152, 175, 253, 560, 659, 830, 924, 1000, 1156
CviQI GTAC 3 cut(s) 294, 1249, 1305
DdeI CTNAG 8 cut(s) 167, 242, 410, 425, 702, 872, 919, 1080
DpnI GATC 6 cut(s) 261, 415, 807, 1191, 1211, 1290
DpnII GATC 6 cut(s) 259, 413, 805, 1189, 1209, 1288
EaeI YGGCCR 1 cut(s) 526
Eco130I CCWWGG 4 cut(s) 151, 999, 1005, 1257
Eco31I GGTCTC 1 cut(s) 537
Eco47I GGWCC 1 cut(s) 445
Eco57I CTGAAG 2 cut(s) 863, 1340
EcoO109I RGGNCCY 2 cut(s) 140, 445
EcoT14I CCWWGG 4 cut(s) 151, 999, 1005, 1257
EcoT22I ATGCAT 1 cut(s) 986
ErhI CCWWGG 4 cut(s) 151, 999, 1005, 1257
FaeI CATG 9 cut(s) 155, 178, 256, 563, 662, 833, 927, 1003, 1159
FaqI GGGAC 2 cut(s) 185, 458
FatI CATG 9 cut(s) 151, 174, 252, 559, 658, 829, 923, 999, 1155
FbaI TGATCA 1 cut(s) 805
Fnu4HI GCNGC 3 cut(s) 111, 595, 1135
FokI GGATG 6 cut(s) 248, 314, 623, 993, 1106, 1121
Fsp4HI GCNGC 3 cut(s) 111, 595, 1135
FspBI CTAG 3 cut(s) 332, 1130, 1316
GluI GCNGC 3 cut(s) 111, 595, 1135
GsaI CCCAGC 1 cut(s) 87
HaeIII GGCC 5 cut(s) 142, 182, 528, 613, 1004
HapII CCGG 3 cut(s) 291, 610, 615
HgaI GACGC 1 cut(s) 347
Hin1I GRCGYC 1 cut(s) 339
Hin1II CATG 9 cut(s) 155, 178, 256, 563, 662, 833, 927, 1003, 1159
HincII GTYRAC 2 cut(s) 337, 909
HindII GTYRAC 2 cut(s) 337, 909
HindIII AAGCTT 1 cut(s) 947
HinfI GANTC 2 cut(s) 1063, 1320
HpaII CCGG 3 cut(s) 291, 610, 615
HphI GGTGA 2 cut(s) 408, 629
Hpy166II GTNNAC 5 cut(s) 337, 395, 909, 1153, 1279
Hpy188I TCNGA 4 cut(s) 376, 646, 1081, 1101
Hpy188III TCNNGA 7 cut(s) 253, 266, 626, 668, 718, 1025, 1217
Hpy8I GTNNAC 5 cut(s) 337, 395, 909, 1153, 1279
HpyAV CCTTC 2 cut(s) 838, 1364
HpyCH4III ACNGT 8 cut(s) 16, 274, 420, 436, 456, 584, 786, 1243
HpyCH4IV ACGT 2 cut(s) 217, 911
HpyCH4V TGCA 7 cut(s) 41, 213, 395, 833, 851, 984, 1044
HpyF10VI GCNNNNNNNGC 3 cut(s) 93, 534, 603
HpyF3I CTNAG 8 cut(s) 167, 242, 410, 425, 702, 872, 919, 1080
HpySE526I ACGT 2 cut(s) 217, 911
Hsp92I GRCGYC 1 cut(s) 339
Hsp92II CATG 9 cut(s) 155, 178, 256, 563, 662, 833, 927, 1003, 1159
KpnI GGTACC 2 cut(s) 297, 1252
Ksp22I TGATCA 1 cut(s) 805
Kzo9I GATC 6 cut(s) 259, 413, 805, 1189, 1209, 1288
LmnI GCTCC 2 cut(s) 96, 246
Lsp1109I GCAGC 3 cut(s) 122, 581, 1146
LweI GCATC 1 cut(s) 971
MaeI CTAG 3 cut(s) 332, 1130, 1316
MaeII ACGT 2 cut(s) 217, 911
MaeIII GTNAC 1 cut(s) 305
MalI GATC 6 cut(s) 261, 415, 807, 1191, 1211, 1290
MboI GATC 6 cut(s) 259, 413, 805, 1189, 1209, 1288
MboII GAAGA 6 cut(s) 473, 476, 908, 1097, 1111, 1337
MflI RGATCY 2 cut(s) 1209, 1288
MhlI GDGCHC 1 cut(s) 397
MlsI TGGCCA 1 cut(s) 528
MluCI AATT 8 cut(s) 280, 301, 351, 370, 799, 819, 1332, 1341
MluNI TGGCCA 1 cut(s) 528
MmeI TCCRAC 1 cut(s) 438
Mox20I TGGCCA 1 cut(s) 528
Mph1103I ATGCAT 1 cut(s) 986
MroXI GAANNNNTTC 3 cut(s) 273, 651, 740
MscI TGGCCA 1 cut(s) 528
MseI TTAA 8 cut(s) 300, 690, 822, 915, 1074, 1182, 1224, 1375
Msp20I TGGCCA 1 cut(s) 528
MspI CCGG 3 cut(s) 291, 610, 615
MspR9I CCNGG 1 cut(s) 615
Mva1269I GAATGC 2 cut(s) 43, 1361
MwoI GCNNNNNNNGC 3 cut(s) 93, 534, 603
NciI CCSGG 1 cut(s) 615
NcoI CCATGG 2 cut(s) 151, 999
NdeII GATC 6 cut(s) 259, 413, 805, 1189, 1209, 1288
NlaIII CATG 9 cut(s) 155, 178, 256, 563, 662, 833, 927, 1003, 1159
NlaIV GGNNCC 4 cut(s) 81, 295, 446, 1250
NmuCI GTSAC 1 cut(s) 305
NsiI ATGCAT 1 cut(s) 986
NspI RCATGY 2 cut(s) 563, 927
NspV TTCGAA 1 cut(s) 991
PagI TCATGA 1 cut(s) 252
PctI GAATGC 2 cut(s) 43, 1361
PdmI GAANNNNTTC 3 cut(s) 273, 651, 740
PfeI GAWTC 2 cut(s) 1063, 1320
PflMI CCANNNNNTGG 1 cut(s) 1258
PkrI GCNGC 3 cut(s) 112, 596, 1136
PpuMI RGGWCCY 1 cut(s) 445
PshBI ATTAAT 1 cut(s) 300
PsiI TTATAA 2 cut(s) 890, 1236
Psp5II RGGWCCY 1 cut(s) 445
PspFI CCCAGC 1 cut(s) 83
PspN4I GGNNCC 4 cut(s) 81, 295, 446, 1250
PspPI GGNCC 4 cut(s) 140, 181, 445, 612
PspPPI RGGWCCY 1 cut(s) 445
PsuI RGATCY 2 cut(s) 1209, 1288
RsaI GTAC 3 cut(s) 295, 1250, 1306
RsaNI GTAC 3 cut(s) 294, 1249, 1305
SaqAI TTAA 8 cut(s) 300, 690, 822, 915, 1074, 1182, 1224, 1375
SatI GCNGC 3 cut(s) 111, 595, 1135
Sau3AI GATC 6 cut(s) 259, 413, 805, 1189, 1209, 1288
Sau96I GGNCC 4 cut(s) 140, 181, 445, 612
ScrFI CCNGG 1 cut(s) 615
SduI GDGCHC 1 cut(s) 397
SfaNI GCATC 1 cut(s) 971
SfcI CTRYAG 2 cut(s) 73, 1201
SfuI TTCGAA 1 cut(s) 991
SinI GGWCC 1 cut(s) 445
Sse9I AATT 8 cut(s) 280, 301, 351, 370, 799, 819, 1332, 1341
SsiI CCGC 1 cut(s) 94
SspI AATATT 1 cut(s) 197
SspMI CTAG 3 cut(s) 332, 1130, 1316
StyD4I CCNGG 1 cut(s) 613
StyI CCWWGG 4 cut(s) 151, 999, 1005, 1257
TaaI ACNGT 8 cut(s) 16, 274, 420, 436, 456, 584, 786, 1243
TaiI ACGT 2 cut(s) 220, 914
TaqI TCGA 4 cut(s) 265, 312, 991, 1365
TasI AATT 8 cut(s) 280, 301, 351, 370, 799, 819, 1332, 1341
TatI WGTACW 1 cut(s) 1304
TfiI GAWTC 2 cut(s) 1063, 1320
Tru1I TTAA 8 cut(s) 300, 690, 822, 915, 1074, 1182, 1224, 1375
Tru9I TTAA 8 cut(s) 300, 690, 822, 915, 1074, 1182, 1224, 1375
TscAI CASTG 2 cut(s) 751, 1152
TseFI GTSAC 1 cut(s) 305
TseI GCWGC 3 cut(s) 110, 594, 1134
Tsp45I GTSAC 1 cut(s) 305
TspDTI ATGAA 4 cut(s) 120, 241, 337, 1076
TspRI CASTG 2 cut(s) 751, 1152
Van91I CCANNNNNTGG 1 cut(s) 1258
VneI GTGCAC 1 cut(s) 393
VpaK11BI GGWCC 1 cut(s) 445
VspI ATTAAT 1 cut(s) 300
XceI RCATGY 2 cut(s) 563, 927
XcmI CCANNNNNNNNNTGG 1 cut(s) 536
XmnI GAANNNNTTC 3 cut(s) 273, 651, 740
XspI CTAG 3 cut(s) 332, 1130, 1316
Zsp2I ATGCAT 1 cut(s) 986
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.