RLG00000028789

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0000821
Physical Location & Seq
Reverse (-)
28054941 .. 28062910
7970 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028789

Sequence Viewer

Length: 1212 bp
ATGCCCATCGTCGCCTCCAACTCTGTCGGCTCCCTGCCTGTCCTGAAACGCAGCATCGGCTGCTCCGGTGATGTGGAGGTGCTGTCCAGGCTAAGCCGAAGCCAGATGTTGCAAGTGGTGGTGAACTCGCCGGTGGTGCAGATGAGGATGTCGGATCTTGGTGGAGCAGCTGGCTTTGCTGAACAAACAGAGCGTCATCAGGTCTGCCATGGATCCCTCAGTTGCACCCAACGTCCGACCGATGCAGAGCTGAGCATCGTCAGCGAACATCGTCAGCTACTCGGTGACTGCGGAACGAAGTTTTTACTCCAATTCCGGTTTGCTGAGTTTCATTTCAATTGCAGCTTCTGGTCAGGGAAACGAAAGATGGGGGGAAGAAGAAGAAAAAAGGTATTGAGGACCGATGATGACCTCCCCGATTCTTTCCCCAGCACCTCCAGGCTTAAAGTCAAAGATAGAGGCAGATATCTAAAAAGGTCAAAGTCGACTTCACAGCTGGAGGGTCAGAAAACTAAGAGTGAAGCTGTACAAAAGTCCGACAAGCCGGAAAAGTGTAACGGAAAATCTGTTAGGGTAAAACCAAAACATTTCACTGATTTGGTAAGACATTTTATGTGTCCGGAAGAAAAAATGAAGTGGACAGAAAATACTACCAATAATCAATACAATATGAGAAAGCTGACGGCAGCAGGGGTTAACTTTATGCCATTTCCTAAAGATGGACGCTTTGTGGTGAAAGAAGGTAACATTTTAAAGTTAACATGTTTTAAAATGATGGACTTGAAGCTTTCACAGTTTTGTGTGAAGGATGAGACGGAATGTATTATTCGCAATGTCATGGCAGTTGAGCAGTTTCTGTATCCAGATGATGCTTATGTTTGCAATTACTTCTTGCTGATGGATAAACTTGTGGACACTGTGGAAGATGTGGATTTGCTGATTCGGAAAAAAGTGATCATTAACATGCTAGGCAGCAAGGAAGCAGTGGCGGAACTGGTGAACCACCTATGTGATCAGATTCTCGAAGAAAGATCCTGCTATGTTGATATTTGTAAAAACCTAAATGACCACTACGAGAATTCTTGGAACCGTTGCGTGACAACCCTGAAACGAGTTTACTTCAAGGATCTGTGGACAGGCAGTTCAACTTTTTTCGGGGTTGTTGTACTGATTTTCTCCATCATTGGATCCATTAAGTCATTCCAAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

404

Amino Acids

46.05

Weight (kDa)

9.19

Isoelectric Point (pI)

40.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 177 - 392 7.1e-47 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 485
AccIII TCCGGA 1 cut(s) 619
AciI CCGC 2 cut(s) 291, 989
AclWI GGATC 7 cut(s) 162, 207, 220, 1026, 1134, 1182, 1195
AcsI RAATTY 1 cut(s) 1078
AfaI GTAC 2 cut(s) 528, 1167
AfiI CCNNNNNNNGG 1 cut(s) 719
AflIII ACRYGT 1 cut(s) 761
AgsI TTSAA 4 cut(s) 337, 784, 1123, 1146
AjnI CCWGG 2 cut(s) 86, 437
AleI CACNNNNGTG 1 cut(s) 1008
AloI GAACNNNNNNTCC 2 cut(s) 1126, 1158
AluBI AGCT 8 cut(s) 170, 250, 277, 345, 496, 524, 679, 787
AluI AGCT 8 cut(s) 170, 250, 277, 345, 496, 524, 679, 787
Alw26I GTCTC 1 cut(s) 806
AlwI GGATC 7 cut(s) 162, 207, 220, 1026, 1134, 1182, 1195
AlwNI CAGNNNCTG 2 cut(s) 348, 856
Aor13HI TCCGGA 1 cut(s) 619
ApeKI GCWGC 6 cut(s) 51, 60, 167, 342, 686, 972
ApoI RAATTY 1 cut(s) 1078
ArsI GACNNNNNNTTYG 2 cut(s) 178, 210
AspS9I GGNCC 1 cut(s) 399
AsuHPI GGTGA 5 cut(s) 80, 133, 296, 745, 1009
AvaII GGWCC 1 cut(s) 399
BamHI GGATCC 2 cut(s) 212, 1187
BbvI GCAGC 6 cut(s) 47, 63, 179, 354, 698, 984
BccI CCATC 6 cut(s) 14, 361, 713, 769, 892, 1187
BceAI ACGGC 1 cut(s) 699
BciT130I CCWGG 2 cut(s) 88, 439
BciVI GTATCC 1 cut(s) 870
BclI TGATCA 2 cut(s) 954, 1012
BcoDI GTCTC 1 cut(s) 806
BfaI CTAG 1 cut(s) 968
BfuI GTATCC 1 cut(s) 870
BisI GCNGC 6 cut(s) 52, 61, 168, 343, 687, 973
BlpI GCTNAGC 2 cut(s) 92, 251
BlsI GCNGC 6 cut(s) 53, 62, 169, 344, 688, 974
Bme1390I CCNGG 2 cut(s) 88, 439
Bme18I GGWCC 1 cut(s) 399
BmgT120I GGNCC 1 cut(s) 399
BmiI GGNNCC 4 cut(s) 31, 214, 1088, 1189
BmrFI CCNGG 2 cut(s) 88, 439
BmsI GCATC 4 cut(s) 63, 232, 264, 859
BpmI CTGGAG 2 cut(s) 421, 518
Bpu1102I GCTNAGC 2 cut(s) 92, 251
BsaJI CCNNGG 1 cut(s) 208
BsaWI WCCGGW 3 cut(s) 65, 315, 619
BsaXI ACNNNNNCTCC 2 cut(s) 68, 98
Bsc4I CCNNNNNNNGG 1 cut(s) 719
Bse118I RCCGGY 1 cut(s) 130
Bse1I ACTGG 1 cut(s) 999
Bse3DI GCAATG 1 cut(s) 838
BseAI TCCGGA 1 cut(s) 619
BseBI CCWGG 2 cut(s) 88, 439
BseDI CCNNGG 1 cut(s) 208
BseGI GGATG 2 cut(s) 153, 814
BseLI CCNNNNNNNGG 1 cut(s) 719
BseMI GCAATG 1 cut(s) 838
BseMII CTCAG 3 cut(s) 232, 242, 315
BseNI ACTGG 1 cut(s) 999
BseXI GCAGC 6 cut(s) 47, 63, 179, 354, 698, 984
BseYI CCCAGC 1 cut(s) 428
BsgI GTGCAG 1 cut(s) 158
Bsh1285I CGRYCG 1 cut(s) 240
BsiEI CGRYCG 1 cut(s) 240
BsiSI CCGG 5 cut(s) 66, 131, 316, 545, 620
BslI CCNNNNNNNGG 1 cut(s) 719
BsmAI GTCTC 1 cut(s) 806
BsmBI CGTCTC 1 cut(s) 806
Bsp13I TCCGGA 1 cut(s) 619
Bsp1407I TGTACA 1 cut(s) 526
Bsp143I GATC 7 cut(s) 154, 212, 954, 1012, 1031, 1126, 1187
Bsp1720I GCTNAGC 2 cut(s) 92, 251
Bsp19I CCATGG 1 cut(s) 208
BspACI CCGC 2 cut(s) 291, 989
BspCNI CTCAG 3 cut(s) 231, 243, 316
BspEI TCCGGA 1 cut(s) 619
BspLI GGNNCC 4 cut(s) 31, 214, 1088, 1189
BspPI GGATC 7 cut(s) 162, 207, 220, 1026, 1134, 1182, 1195
BsrDI GCAATG 1 cut(s) 838
BsrFI RCCGGY 1 cut(s) 130
BsrGI TGTACA 1 cut(s) 526
BsrI ACTGG 1 cut(s) 999
BssAI RCCGGY 1 cut(s) 130
BssECI CCNNGG 1 cut(s) 208
BssMI GATC 7 cut(s) 154, 212, 954, 1012, 1031, 1126, 1187
BssT1I CCWWGG 1 cut(s) 208
Bst2UI CCWGG 2 cut(s) 88, 439
Bst4CI ACNGT 3 cut(s) 795, 919, 1091
BstAPI GCANNNNNTGC 1 cut(s) 60
BstAUI TGTACA 1 cut(s) 526
BstC8I GCNNGC 1 cut(s) 172
BstDEI CTNAG 5 cut(s) 92, 218, 251, 324, 513
BstDSI CCRYGG 1 cut(s) 208
BstF5I GGATG 2 cut(s) 153, 814
BstKTI GATC 7 cut(s) 157, 215, 957, 1015, 1034, 1129, 1190
BstMAI GTCTC 1 cut(s) 806
BstMBI GATC 7 cut(s) 154, 212, 954, 1012, 1031, 1126, 1187
BstMCI CGRYCG 1 cut(s) 240
BstMWI GCNNNNNNNGC 6 cut(s) 57, 60, 88, 136, 176, 261
BstNI CCWGG 2 cut(s) 88, 439
BstNSI RCATGY 2 cut(s) 765, 967
BstSCI CCNGG 2 cut(s) 86, 437
BstV1I GCAGC 6 cut(s) 47, 63, 179, 354, 698, 984
BstX2I RGATCY 5 cut(s) 154, 212, 1031, 1126, 1187
BstYI RGATCY 5 cut(s) 154, 212, 1031, 1126, 1187
BsuI GTATCC 1 cut(s) 870
BtgI CCRYGG 1 cut(s) 208
BtsCI GGATG 2 cut(s) 153, 814
BtsI GCAGTG 1 cut(s) 990
BtsIMutI CAGTG 3 cut(s) 591, 915, 990
Cac8I GCNNGC 1 cut(s) 172
CaiI CAGNNNCTG 2 cut(s) 348, 856
Cfr10I RCCGGY 1 cut(s) 130
Cfr13I GGNCC 1 cut(s) 399
CseI GACGC 2 cut(s) 182, 732
Csp6I GTAC 2 cut(s) 527, 1166
CviAII CATG 4 cut(s) 209, 762, 838, 964
CviQI GTAC 2 cut(s) 527, 1166
DdeI CTNAG 5 cut(s) 92, 218, 251, 324, 513
DpnI GATC 7 cut(s) 156, 214, 956, 1014, 1033, 1128, 1189
DpnII GATC 7 cut(s) 154, 212, 954, 1012, 1031, 1126, 1187
DraI TTTAAA 2 cut(s) 753, 769
EciI GGCGGA 1 cut(s) 1004
Eco130I CCWWGG 1 cut(s) 208
Eco32I GATATC 1 cut(s) 467
Eco47I GGWCC 1 cut(s) 399
EcoRI GAATTC 1 cut(s) 1078
EcoRII CCWGG 2 cut(s) 86, 437
EcoRV GATATC 1 cut(s) 467
EcoT14I CCWWGG 1 cut(s) 208
ErhI CCWWGG 1 cut(s) 208
Esp3I CGTCTC 1 cut(s) 806
FaeI CATG 4 cut(s) 212, 765, 841, 967
FatI CATG 4 cut(s) 208, 761, 837, 963
FbaI TGATCA 2 cut(s) 954, 1012
FblI GTMKAC 1 cut(s) 485
Fnu4HI GCNGC 6 cut(s) 52, 61, 168, 343, 687, 973
FokI GGATG 2 cut(s) 160, 821
Fsp4HI GCNGC 6 cut(s) 52, 61, 168, 343, 687, 973
FspBI CTAG 1 cut(s) 968
GluI GCNGC 6 cut(s) 52, 61, 168, 343, 687, 973
GsaI CCCAGC 1 cut(s) 432
GsuI CTGGAG 2 cut(s) 421, 518
HapII CCGG 5 cut(s) 66, 131, 316, 545, 620
HgaI GACGC 2 cut(s) 182, 732
Hin1II CATG 4 cut(s) 212, 765, 841, 967
HincII GTYRAC 3 cut(s) 486, 697, 759
HindII GTYRAC 3 cut(s) 486, 697, 759
HindIII AAGCTT 1 cut(s) 785
HinfI GANTC 3 cut(s) 419, 940, 1018
HpaI GTTAAC 2 cut(s) 697, 759
HpaII CCGG 5 cut(s) 66, 131, 316, 545, 620
HphI GGTGA 5 cut(s) 80, 133, 296, 745, 1009
Hpy166II GTNNAC 9 cut(s) 124, 486, 639, 697, 759, 913, 1000, 1117, 1134
Hpy188I TCNGA 6 cut(s) 154, 237, 507, 538, 945, 1017
Hpy188III TCNNGA 4 cut(s) 43, 620, 863, 1022
Hpy8I GTNNAC 9 cut(s) 124, 486, 639, 697, 759, 913, 1000, 1117, 1134
Hpy99I CGWCG 1 cut(s) 14
HpyAV CCTTC 2 cut(s) 734, 799
HpyCH4III ACNGT 3 cut(s) 795, 919, 1091
HpyCH4IV ACGT 1 cut(s) 232
HpyCH4V TGCA 6 cut(s) 112, 139, 225, 245, 342, 882
HpyF10VI GCNNNNNNNGC 6 cut(s) 57, 60, 88, 136, 176, 261
HpyF3I CTNAG 5 cut(s) 92, 218, 251, 324, 513
HpySE526I ACGT 1 cut(s) 232
Hsp92II CATG 4 cut(s) 212, 765, 841, 967
Kpn2I TCCGGA 1 cut(s) 619
Ksp22I TGATCA 2 cut(s) 954, 1012
KspAI GTTAAC 2 cut(s) 697, 759
Kzo9I GATC 7 cut(s) 154, 212, 954, 1012, 1031, 1126, 1187
LmnI GCTCC 3 cut(s) 35, 68, 164
Lsp1109I GCAGC 6 cut(s) 47, 63, 179, 354, 698, 984
LweI GCATC 4 cut(s) 63, 232, 264, 859
MaeI CTAG 1 cut(s) 968
MaeII ACGT 1 cut(s) 232
MaeIII GTNAC 4 cut(s) 284, 554, 743, 1096
MalI GATC 7 cut(s) 156, 214, 956, 1014, 1033, 1128, 1189
MboI GATC 7 cut(s) 154, 212, 954, 1012, 1031, 1126, 1187
MboII GAAGA 6 cut(s) 387, 390, 393, 635, 935, 1037
MfeI CAATTG 1 cut(s) 337
MflI RGATCY 5 cut(s) 154, 212, 1031, 1126, 1187
MluCI AATT 4 cut(s) 311, 337, 883, 1078
MmeI TCCRAC 4 cut(s) 42, 132, 260, 561
MnlI CCTC 9 cut(s) 25, 70, 138, 227, 390, 422, 445, 452, 493
MroI TCCGGA 1 cut(s) 619
MseI TTAA 7 cut(s) 444, 696, 752, 758, 768, 960, 1194
MslI CAYNNNNRTG 2 cut(s) 962, 1008
MspA1I CMGCKG 2 cut(s) 170, 496
MspI CCGG 5 cut(s) 66, 131, 316, 545, 620
MspR9I CCNGG 2 cut(s) 88, 439
MunI CAATTG 1 cut(s) 337
MvaI CCWGG 2 cut(s) 88, 439
MwoI GCNNNNNNNGC 6 cut(s) 57, 60, 88, 136, 176, 261
NcoI CCATGG 1 cut(s) 208
NdeII GATC 7 cut(s) 154, 212, 954, 1012, 1031, 1126, 1187
NlaIII CATG 4 cut(s) 212, 765, 841, 967
NlaIV GGNNCC 4 cut(s) 31, 214, 1088, 1189
NmuCI GTSAC 2 cut(s) 284, 1096
NspI RCATGY 2 cut(s) 765, 967
OliI CACNNNNGTG 1 cut(s) 1008
PciI ACATGT 1 cut(s) 761
PfeI GAWTC 3 cut(s) 419, 940, 1018
PkrI GCNGC 6 cut(s) 53, 62, 169, 344, 688, 974
PscI ACATGT 1 cut(s) 761
Psp6I CCWGG 2 cut(s) 86, 437
PspFI CCCAGC 1 cut(s) 428
PspGI CCWGG 2 cut(s) 86, 437
PspN4I GGNNCC 4 cut(s) 31, 214, 1088, 1189
PspPI GGNCC 1 cut(s) 399
PstNI CAGNNNCTG 2 cut(s) 348, 856
PsuI RGATCY 5 cut(s) 154, 212, 1031, 1126, 1187
PvuII CAGCTG 2 cut(s) 170, 496
RsaI GTAC 2 cut(s) 528, 1167
RsaNI GTAC 2 cut(s) 527, 1166
RseI CAYNNNNRTG 2 cut(s) 962, 1008
SalI GTCGAC 1 cut(s) 484
SaqAI TTAA 7 cut(s) 444, 696, 752, 758, 768, 960, 1194
SatI GCNGC 6 cut(s) 52, 61, 168, 343, 687, 973
Sau3AI GATC 7 cut(s) 154, 212, 954, 1012, 1031, 1126, 1187
Sau96I GGNCC 1 cut(s) 399
ScrFI CCNGG 2 cut(s) 88, 439
SfaNI GCATC 4 cut(s) 63, 232, 264, 859
SgrAI CRCCGGYG 1 cut(s) 130
SinI GGWCC 1 cut(s) 399
SmiMI CAYNNNNRTG 2 cut(s) 962, 1008
Sse9I AATT 4 cut(s) 311, 337, 883, 1078
SsiI CCGC 2 cut(s) 291, 989
SspMI CTAG 1 cut(s) 968
StyD4I CCNGG 2 cut(s) 86, 437
StyI CCWWGG 1 cut(s) 208
TaaI ACNGT 3 cut(s) 795, 919, 1091
TaiI ACGT 1 cut(s) 235
TaqI TCGA 2 cut(s) 485, 1023
TaqII GACCGA 2 cut(s) 254, 416
TasI AATT 4 cut(s) 311, 337, 883, 1078
TatI WGTACW 2 cut(s) 526, 1165
TfiI GAWTC 3 cut(s) 419, 940, 1018
Tru1I TTAA 7 cut(s) 444, 696, 752, 758, 768, 960, 1194
Tru9I TTAA 7 cut(s) 444, 696, 752, 758, 768, 960, 1194
TscAI CASTG 3 cut(s) 598, 922, 990
TseFI GTSAC 2 cut(s) 284, 1096
TseI GCWGC 6 cut(s) 51, 60, 167, 342, 686, 972
Tsp45I GTSAC 2 cut(s) 284, 1096
TspDTI ATGAA 2 cut(s) 320, 647
TspGWI ACGGA 2 cut(s) 573, 830
TspRI CASTG 3 cut(s) 598, 922, 990
VpaK11BI GGWCC 1 cut(s) 399
XapI RAATTY 1 cut(s) 1078
XceI RCATGY 2 cut(s) 765, 967
XmiI GTMKAC 1 cut(s) 485
XspI CTAG 1 cut(s) 968
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.