FvH4_7g09710

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0000821
Physical Location & Seq
Forward (+)
9317794 .. 9319077
1284 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g09710.t1

Sequence Viewer

Length: 1284 bp
ATGAATGGAGCAAGTGATCACGTCGCGATCAAGCGCACCGAAACAAAACCATTCTTGGACGAGTGGTGTATCTACAGGGTTCCCAGCAAACTGCGCAACGTAAATAAAGCATCATACACTCCTCAATTACTTTCGATAGGCCCTTTCCACTATGGCAGTCCAGAACTGAGGGACATGGAAAGCCATAAACAGAAGTATTATGAGAAATTTCTCAGACGGTGTTCAAAGAATGAGGCTGAGCTGAAAAGCTTGGTGAGCCAACATCAGGCCAGAATTCTTAGTTGTTATGCAGGTACCATCGAGTGCAGAGGCAAATTCGTGAACATGATTCTAGTTGATGCTTGCTTCATCATTGAACTATTTATAACAGACTCTGAAACAGAAAATCATCACATACTGAGGTCACCTTGGATGAGGAAAGCTGTAGAGACAGACTTGATACTGTTTGAAAATCAGATTCCTTATTATTTTCTTCAAGAGCTTTATGACTTTGCCATTTCCAATGCTGAACCTAACATTGCCGAGGACCTTACACCTGATCCACATCGATTCCGTACTCTTTCTTGTAAGTTTTTTAAAGAATATAACAAGAAGAAAAGAGTTCTCGGCAATGGAGTAAAGCTGAATCATTTCACTGATTTGGTTAGACTATTTGTGTGTACAGACAAAGTACAAGGTGACTGTGTCAGTAACCAAAAGAAGTGTAAATATGATGTGAGAAAGCTCAAGGCAGCAGGAGTAAAGTTTACGCACCTTGCAGAGCCCTTTGTAATAGAGTCTTTGTCAACAGGAGTTAGCAGCTGTAGATCCAAGTTAGCATGTTGTCTTACAACCAAGGAGTTGAAGCTTACACGTTTCTGGGCAACATATGATGCTGAGAGCGTCATAAGAAACATTATGGCCTTGGAACAACTCATCTACCCGAAAAAGGCTTATATTTGCAGCTATTTTTTGCTACTTAGTCAACTTGTCAACACTGTGGAAGACATAGATTTGCTGATTAAGGACGGAGTGGTCGAGAACTTGCTAGGCAGCAACAAATCGGTCGAAAAGCTTATTAACTCATTCTGTGTGCACATTGTGGAGGAAATATCCTGCTATTCGGATATCTATGACCAGCTTAATGAGCACTACGAGACCAGTTTATGCAACCGCCGTATGGCAACCCTGAAACAAGTTTACTTCAAGGATCTTTGGACAAGCAGTGCAACTATACTTGGACTGGTTGTCCTGGTTTTCTCAATTGTTGGTACTATCAATTCGCTCATCTATCACAAGCGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

428

Amino Acids

49.29

Weight (kDa)

8.55

Isoelectric Point (pI)

51.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 24 - 413 6.8e-87 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 365
AasI GACNNNNNNGTC 1 cut(s) 1013
Acc16I TGCGCA 1 cut(s) 95
Acc36I ACCTGC 1 cut(s) 281
Acc65I GGTACC 1 cut(s) 293
AccB1I GGYRCC 1 cut(s) 293
AccII CGCG 1 cut(s) 26
AciI CCGC 1 cut(s) 1153
AclWI GGATC 3 cut(s) 533, 801, 1197
AcsI RAATTY 3 cut(s) 206, 273, 314
AdeI CACNNNGTG 1 cut(s) 1081
AfaI GTAC 5 cut(s) 295, 556, 661, 672, 1252
AfiI CCNNNNNNNGG 3 cut(s) 265, 928, 1159
AflIII ACRYGT 1 cut(s) 851
AgsI TTSAA 6 cut(s) 225, 356, 449, 476, 844, 1186
AhdI GACNNNNNGTC 1 cut(s) 1226
AjiI CACGTC 1 cut(s) 22
AjnI CCWGG 1 cut(s) 1230
Alw21I GWGCWC 2 cut(s) 1077, 1131
Alw26I GTCTC 2 cut(s) 422, 1130
Alw44I GTGCAC 1 cut(s) 1073
AlwI GGATC 3 cut(s) 533, 801, 1197
AlwNI CAGNNNCTG 1 cut(s) 374
AoxI GGCC 3 cut(s) 139, 267, 900
ApaLI GTGCAC 1 cut(s) 1073
ApeKI GCWGC 4 cut(s) 731, 798, 942, 1032
ApoI RAATTY 3 cut(s) 206, 273, 314
Asp700I GAANNNNTTC 1 cut(s) 629
Asp718I GGTACC 1 cut(s) 293
AspLEI GCGC 2 cut(s) 36, 96
AspS9I GGNCC 2 cut(s) 140, 526
AsuHPI GGTGA 3 cut(s) 265, 396, 689
AvaII GGWCC 1 cut(s) 526
BaeGI GKGCMC 1 cut(s) 1077
BanI GGYRCC 1 cut(s) 293
BanII GRGCYC 1 cut(s) 765
BbsI GAAGAC 1 cut(s) 990
Bbv12I GWGCWC 2 cut(s) 1077, 1131
BbvI GCAGC 4 cut(s) 743, 810, 954, 1044
BccI CCATC 1 cut(s) 305
BceAI ACGGC 1 cut(s) 1140
BciT130I CCWGG 1 cut(s) 1232
BclI TGATCA 1 cut(s) 16
BcoDI GTCTC 2 cut(s) 422, 1130
BfaI CTAG 2 cut(s) 332, 1028
BfmI CTRYAG 3 cut(s) 73, 423, 802
BfuAI ACCTGC 1 cut(s) 281
BisI GCNGC 4 cut(s) 732, 799, 943, 1033
BlpI GCTNAGC 1 cut(s) 237
BlsI GCNGC 4 cut(s) 733, 800, 944, 1034
Bme1390I CCNGG 1 cut(s) 1232
Bme18I GGWCC 1 cut(s) 526
BmeRI GACNNNNNGTC 1 cut(s) 1226
BmgBI CACGTC 1 cut(s) 22
BmgT120I GGNCC 2 cut(s) 140, 526
BmiI GGNNCC 2 cut(s) 81, 295
BmrFI CCNGG 1 cut(s) 1232
BmsI GCATC 3 cut(s) 119, 328, 862
BpiI GAAGAC 1 cut(s) 990
Bpu1102I GCTNAGC 1 cut(s) 237
BpuEI CTTGAG 1 cut(s) 710
Bsa29I ATCGAT 1 cut(s) 547
BsaBI GATNNNNATC 1 cut(s) 543
BsaI GGTCTC 1 cut(s) 1130
BsaJI CCNNGG 4 cut(s) 407, 522, 834, 903
Bsc4I CCNNNNNNNGG 3 cut(s) 265, 928, 1159
Bse1I ACTGG 2 cut(s) 1140, 1227
Bse3DI GCAATG 2 cut(s) 516, 616
Bse8I GATNNNNATC 1 cut(s) 543
BseBI CCWGG 1 cut(s) 1232
BseCI ATCGAT 1 cut(s) 547
BseDI CCNNGG 4 cut(s) 407, 522, 834, 903
BseGI GGATG 1 cut(s) 417
BseJI GATNNNNATC 1 cut(s) 543
BseLI CCNNNNNNNGG 3 cut(s) 265, 928, 1159
BseMI GCAATG 2 cut(s) 516, 616
BseMII CTCAG 5 cut(s) 158, 226, 228, 389, 867
BseNI ACTGG 2 cut(s) 1140, 1227
BseRI GAGGAG 1 cut(s) 111
BseSI GKGCMC 1 cut(s) 1077
BseXI GCAGC 4 cut(s) 743, 810, 954, 1044
BseYI CCCAGC 1 cut(s) 83
BsgI GTGCAG 1 cut(s) 325
Bsh1236I CGCG 1 cut(s) 26
Bsh1285I CGRYCG 1 cut(s) 1047
BshFI GGCC 3 cut(s) 141, 269, 902
BshNI GGYRCC 1 cut(s) 293
BshVI ATCGAT 1 cut(s) 547
BsiEI CGRYCG 1 cut(s) 1047
BsiHKAI GWGCWC 2 cut(s) 1077, 1131
BslFI GGGAC 1 cut(s) 185
BslI CCNNNNNNNGG 3 cut(s) 265, 928, 1159
BsmAI GTCTC 2 cut(s) 422, 1130
BsmFI GGGAC 1 cut(s) 185
BsnI GGCC 3 cut(s) 141, 269, 902
Bso31I GGTCTC 1 cut(s) 1130
Bsp1286I GDGCHC 3 cut(s) 765, 1077, 1131
Bsp1407I TGTACA 1 cut(s) 659
Bsp143I GATC 5 cut(s) 16, 27, 538, 806, 1189
Bsp1720I GCTNAGC 1 cut(s) 237
Bsp68I TCGCGA 1 cut(s) 26
BspACI CCGC 1 cut(s) 1153
BspANI GGCC 3 cut(s) 141, 269, 902
BspCNI CTCAG 5 cut(s) 159, 225, 229, 390, 868
BspDI ATCGAT 1 cut(s) 547
BspFNI CGCG 1 cut(s) 26
BspLI GGNNCC 2 cut(s) 81, 295
BspMI ACCTGC 1 cut(s) 281
BspPI GGATC 3 cut(s) 533, 801, 1197
BspT107I GGYRCC 1 cut(s) 293
BspTNI GGTCTC 1 cut(s) 1130
BsrDI GCAATG 2 cut(s) 516, 616
BsrGI TGTACA 1 cut(s) 659
BsrI ACTGG 2 cut(s) 1140, 1227
BssECI CCNNGG 4 cut(s) 407, 522, 834, 903
BssMI GATC 5 cut(s) 16, 27, 538, 806, 1189
BssT1I CCWWGG 3 cut(s) 407, 834, 903
Bst2UI CCWGG 1 cut(s) 1232
Bst4CI ACNGT 4 cut(s) 219, 444, 683, 979
BstAUI TGTACA 1 cut(s) 659
BstC8I GCNNGC 1 cut(s) 343
BstDEI CTNAG 7 cut(s) 167, 212, 237, 278, 398, 876, 959
BstEII GGTNACC 1 cut(s) 402
BstF5I GGATG 1 cut(s) 417
BstFNI CGCG 1 cut(s) 26
BstHHI GCGC 2 cut(s) 36, 96
BstKTI GATC 5 cut(s) 19, 30, 541, 809, 1192
BstMAI GTCTC 2 cut(s) 422, 1130
BstMBI GATC 5 cut(s) 16, 27, 538, 806, 1189
BstMCI CGRYCG 1 cut(s) 1047
BstMWI GCNNNNNNNGC 3 cut(s) 93, 255, 1126
BstNI CCWGG 1 cut(s) 1232
BstNSI RCATGY 1 cut(s) 822
BstPI GGTNACC 1 cut(s) 402
BstSCI CCNGG 1 cut(s) 1230
BstSFI CTRYAG 3 cut(s) 73, 423, 802
BstSLI GKGCMC 1 cut(s) 1077
BstUI CGCG 1 cut(s) 26
BstV1I GCAGC 4 cut(s) 743, 810, 954, 1044
BstV2I GAAGAC 1 cut(s) 990
BstX2I RGATCY 2 cut(s) 806, 1189
BstYI RGATCY 2 cut(s) 806, 1189
Bsu15I ATCGAT 1 cut(s) 547
BsuRI GGCC 3 cut(s) 141, 269, 902
BsuTUI ATCGAT 1 cut(s) 547
BtrI CACGTC 1 cut(s) 22
BtsCI GGATG 1 cut(s) 417
BtsI GCAGTG 1 cut(s) 1210
BtsIMutI CAGTG 3 cut(s) 633, 975, 1210
BtuMI TCGCGA 1 cut(s) 26
BveI ACCTGC 1 cut(s) 281
Cac8I GCNNGC 1 cut(s) 343
CaiI CAGNNNCTG 1 cut(s) 374
CfoI GCGC 2 cut(s) 36, 96
Cfr13I GGNCC 2 cut(s) 140, 526
ClaI ATCGAT 1 cut(s) 547
CseI GACGC 1 cut(s) 871
Csp6I GTAC 5 cut(s) 294, 555, 660, 671, 1251
CviAII CATG 3 cut(s) 175, 325, 819
CviQI GTAC 5 cut(s) 294, 555, 660, 671, 1251
DdeI CTNAG 7 cut(s) 167, 212, 237, 278, 398, 876, 959
DpnI GATC 5 cut(s) 18, 29, 540, 808, 1191
DpnII GATC 5 cut(s) 16, 27, 538, 806, 1189
DraI TTTAAA 1 cut(s) 577
DraIII CACNNNGTG 1 cut(s) 1081
DrdI GACNNNNNNGTC 1 cut(s) 1013
DriI GACNNNNNGTC 1 cut(s) 1226
DseDI GACNNNNNNGTC 1 cut(s) 1013
Eam1105I GACNNNNNGTC 1 cut(s) 1226
Eco130I CCWWGG 3 cut(s) 407, 834, 903
Eco24I GRGCYC 1 cut(s) 765
Eco31I GGTCTC 1 cut(s) 1130
Eco32I GATATC 1 cut(s) 1108
Eco47I GGWCC 1 cut(s) 526
Eco91I GGTNACC 1 cut(s) 402
EcoO109I RGGNCCY 2 cut(s) 140, 526
EcoO65I GGTNACC 1 cut(s) 402
EcoRI GAATTC 1 cut(s) 273
EcoRII CCWGG 1 cut(s) 1230
EcoRV GATATC 1 cut(s) 1108
EcoT14I CCWWGG 3 cut(s) 407, 834, 903
EcoT38I GRGCYC 1 cut(s) 765
ErhI CCWWGG 3 cut(s) 407, 834, 903
FaeI CATG 3 cut(s) 178, 328, 822
FaqI GGGAC 1 cut(s) 185
FatI CATG 3 cut(s) 174, 324, 818
FauNDI CATATG 1 cut(s) 868
FbaI TGATCA 1 cut(s) 16
Fnu4HI GCNGC 4 cut(s) 732, 799, 943, 1033
FokI GGATG 1 cut(s) 424
FriOI GRGCYC 1 cut(s) 765
Fsp4HI GCNGC 4 cut(s) 732, 799, 943, 1033
FspBI CTAG 2 cut(s) 332, 1028
FspI TGCGCA 1 cut(s) 95
GlaI GCGC 2 cut(s) 35, 95
GluI GCNGC 4 cut(s) 732, 799, 943, 1033
GsaI CCCAGC 1 cut(s) 87
HaeIII GGCC 3 cut(s) 141, 269, 902
HgaI GACGC 1 cut(s) 871
HhaI GCGC 2 cut(s) 36, 96
Hin1II CATG 3 cut(s) 178, 328, 822
Hin6I GCGC 2 cut(s) 34, 94
HinP1I GCGC 2 cut(s) 34, 94
HincII GTYRAC 3 cut(s) 786, 965, 973
HindII GTYRAC 3 cut(s) 786, 965, 973
HindIII AAGCTT 3 cut(s) 247, 845, 1052
HinfI GANTC 6 cut(s) 328, 371, 457, 549, 625, 776
HphI GGTGA 3 cut(s) 265, 396, 689
Hpy166II GTNNAC 8 cut(s) 322, 660, 747, 786, 965, 973, 1075, 1180
Hpy188I TCNGA 4 cut(s) 215, 376, 456, 1105
Hpy188III TCNNGA 5 cut(s) 25, 161, 319, 476, 1018
Hpy8I GTNNAC 8 cut(s) 322, 660, 747, 786, 965, 973, 1075, 1180
Hpy99I CGWCG 1 cut(s) 26
HpyCH4III ACNGT 4 cut(s) 219, 444, 683, 979
HpyCH4IV ACGT 3 cut(s) 21, 99, 853
HpyCH4V TGCA 7 cut(s) 290, 306, 758, 942, 1075, 1149, 1208
HpyF10VI GCNNNNNNNGC 3 cut(s) 93, 255, 1126
HpyF3I CTNAG 7 cut(s) 167, 212, 237, 278, 398, 876, 959
HpySE526I ACGT 3 cut(s) 21, 99, 853
Hsp92II CATG 3 cut(s) 178, 328, 822
HspAI GCGC 2 cut(s) 34, 94
KpnI GGTACC 1 cut(s) 297
Ksp22I TGATCA 1 cut(s) 16
Kzo9I GATC 5 cut(s) 16, 27, 538, 806, 1189
LmnI GCTCC 1 cut(s) 8
Lsp1109I GCAGC 4 cut(s) 743, 810, 954, 1044
LweI GCATC 3 cut(s) 119, 328, 862
MaeI CTAG 2 cut(s) 332, 1028
MaeII ACGT 3 cut(s) 21, 99, 853
MaeIII GTNAC 3 cut(s) 402, 677, 689
MalI GATC 5 cut(s) 18, 29, 540, 808, 1191
MboI GATC 5 cut(s) 16, 27, 538, 806, 1189
MboII GAAGA 3 cut(s) 464, 604, 995
MfeI CAATTG 1 cut(s) 1242
MflI RGATCY 2 cut(s) 806, 1189
MhlI GDGCHC 3 cut(s) 765, 1077, 1131
MluCI AATT 6 cut(s) 125, 206, 273, 314, 1242, 1258
MlyI GAGTC 2 cut(s) 365, 785
MnlI CCTC 8 cut(s) 132, 162, 226, 302, 393, 408, 517, 1078
MroXI GAANNNNTTC 1 cut(s) 629
MseI TTAA 5 cut(s) 576, 1002, 1059, 1122, 1282
MspA1I CMGCKG 1 cut(s) 801
MspR9I CCNGG 1 cut(s) 1232
MunI CAATTG 1 cut(s) 1242
MvaI CCWGG 1 cut(s) 1232
MvnI CGCG 1 cut(s) 26
MwoI GCNNNNNNNGC 3 cut(s) 93, 255, 1126
NdeI CATATG 1 cut(s) 868
NdeII GATC 5 cut(s) 16, 27, 538, 806, 1189
NlaIII CATG 3 cut(s) 178, 328, 822
NlaIV GGNNCC 2 cut(s) 81, 295
NmeAIII GCCGAG 2 cut(s) 547, 585
NmuCI GTSAC 2 cut(s) 402, 677
NruI TCGCGA 1 cut(s) 26
NsbI TGCGCA 1 cut(s) 95
NspI RCATGY 1 cut(s) 822
PcsI WCGNNNNNNNCGW 1 cut(s) 1014
PdmI GAANNNNTTC 1 cut(s) 629
PfeI GAWTC 4 cut(s) 328, 457, 549, 625
PflFI GACNNNGTC 1 cut(s) 683
PkrI GCNGC 4 cut(s) 733, 800, 944, 1034
PleI GAGTC 2 cut(s) 365, 784
PpsI GAGTC 2 cut(s) 365, 784
PpuMI RGGWCCY 1 cut(s) 526
PsiI TTATAA 1 cut(s) 365
Psp5II RGGWCCY 1 cut(s) 526
Psp6I CCWGG 1 cut(s) 1230
PspEI GGTNACC 1 cut(s) 402
PspFI CCCAGC 1 cut(s) 83
PspGI CCWGG 1 cut(s) 1230
PspN4I GGNNCC 2 cut(s) 81, 295
PspPI GGNCC 2 cut(s) 140, 526
PspPPI RGGWCCY 1 cut(s) 526
PstNI CAGNNNCTG 1 cut(s) 374
PsuI RGATCY 2 cut(s) 806, 1189
PsyI GACNNNGTC 1 cut(s) 683
PvuII CAGCTG 1 cut(s) 801
RruI TCGCGA 1 cut(s) 26
RsaI GTAC 5 cut(s) 295, 556, 661, 672, 1252
RsaNI GTAC 5 cut(s) 294, 555, 660, 671, 1251
SaqAI TTAA 5 cut(s) 576, 1002, 1059, 1122, 1282
SatI GCNGC 4 cut(s) 732, 799, 943, 1033
Sau3AI GATC 5 cut(s) 16, 27, 538, 806, 1189
Sau96I GGNCC 2 cut(s) 140, 526
SchI GAGTC 2 cut(s) 365, 785
ScrFI CCNGG 1 cut(s) 1232
SduI GDGCHC 3 cut(s) 765, 1077, 1131
SfaNI GCATC 3 cut(s) 119, 328, 862
SfcI CTRYAG 3 cut(s) 73, 423, 802
SinI GGWCC 1 cut(s) 526
SmlI CTYRAG 1 cut(s) 725
SmoI CTYRAG 1 cut(s) 725
Sse9I AATT 6 cut(s) 125, 206, 273, 314, 1242, 1258
SsiI CCGC 1 cut(s) 1153
SspMI CTAG 2 cut(s) 332, 1028
StyD4I CCNGG 1 cut(s) 1230
StyI CCWWGG 3 cut(s) 407, 834, 903
TaaI ACNGT 4 cut(s) 219, 444, 683, 979
TaiI ACGT 3 cut(s) 24, 102, 856
TaqI TCGA 5 cut(s) 134, 300, 547, 1017, 1047
TaqII GACCGA 1 cut(s) 1033
TasI AATT 6 cut(s) 125, 206, 273, 314, 1242, 1258
TatI WGTACW 2 cut(s) 659, 670
TfiI GAWTC 4 cut(s) 328, 457, 549, 625
Tru1I TTAA 5 cut(s) 576, 1002, 1059, 1122, 1282
Tru9I TTAA 5 cut(s) 576, 1002, 1059, 1122, 1282
TscAI CASTG 3 cut(s) 640, 982, 1210
TseFI GTSAC 2 cut(s) 402, 677
TseI GCWGC 4 cut(s) 731, 798, 942, 1032
Tsp45I GTSAC 2 cut(s) 402, 677
TspDTI ATGAA 2 cut(s) 17, 337
TspGWI ACGGA 2 cut(s) 542, 1023
TspRI CASTG 3 cut(s) 640, 982, 1210
Tth111I GACNNNGTC 1 cut(s) 683
VneI GTGCAC 1 cut(s) 1073
VpaK11BI GGWCC 1 cut(s) 526
XapI RAATTY 3 cut(s) 206, 273, 314
XceI RCATGY 1 cut(s) 822
XmnI GAANNNNTTC 1 cut(s) 629
XspI CTAG 2 cut(s) 332, 1028
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.