Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4B

Orthogroup ID

OG0000821

Physical Location & Seq

Genomic Coordinates Reverse (-)

2600912 .. 2603756

Gene Structure 2845 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4BG016600.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1377 bp

ATGGTAGAACCTCAGAGTGAACAAAGAGAGAATGCACCCGTATTGTTCTTGCAGGAATGGTGTATCTATAGGGTTCCCAGCCAGCTCCGCAATGTAGATGAAGCAGCATACACTCCTCAAACACTTTCAATAGGGCCTTTCCACCATGGAAATCCAAAACTGAGGGACATGGAGGCCCATAAGAACAAATATTATGAGTTGTTTTGCAGACGTTCTTCGAAAAGTGAGGACGAACTAAGGAGCTTCATGATGGATCATCGAGAAACAATTCTAAATTGTTATGCAGGTACCTTTAAATGTGAGATCGACTTTTTGAACCTCATCCTAGTTGACGCCTGCTTCATAATTGAACTCTTTTTGACTAATTCTGAACAAGAACAACATAATGCAAATTATTATATACTGAGATCACCGTGGCTGAGGAAAACGGTGGAGAGGGACCTGATACTGTTGGAAAATCAGCTTCCTTATTTTCTTCTTCAAAAGCTATATGACTTTGCCATACCTACCTTTTCCAATGGCCATCTTGGCAATGGAGACCAAGCACAAGTTGGGGGACATGTCCATAAAGATAGCACCAACAGTCAGCAATGCTGCTTCCCTTGTTTCCGGCCCGGTGATTTTCAAGAGAACTCCATCCGATCTGAACCTATTCAACATGGCTTTCTTGAACTTACCTGTAAGTTTTTTAAGGCATACACCAAGGGAAAATCTGTCGGGAATGGATTTGAGCCAAAACACTTCACTGATTTGGTTAGACACTTTCTATGCCCGTGCAAGGAGCTGTTGTGGGTCAAAAATTATGATCAGCCCGTCAAAATTAAATACCATGCAAAAAAGCTGAAGGTTGCAGGAGTGAAGTTTATGCCACTTAGAGAACCATTTGTTATAAAGGAAGACCCTGTGTTGACGTTTAACCCAGTATGTTTGGCACATAAGGAGTTGAAGCTTACAAGACTTTGGGCAGAGTATGAGGCAGAATGCATTTTTCGAAACACCATGGCCTTAGAACAACTGATGTATCCAGAAAAGCCTTATATTTGCTGTTATTTTTCGCTGATGAATCAACTCATTAATACTGAGGAAGATGTGGATGTCCTGATTGAGGATGGAGTAATCTTCAACTTGCTAGGCAGCAACAAAGCAGTGGTAAACATGATCAGTTCATTGTGTGAGCAGATTAAGGAGGATTACTCCTGCTATAGTGAGATCTGTCAAGACCTTAATAGTCATTATAAAACAGTTTTGTACCACCCCTTGGTAATAGTGAGACAGGTTTACTTCAAGGATCTTTGGAGAGGCAGTACAGCTATACTAGGATTCGTTGTCCTAATTTTCACAATTCTTCAATCCCTTCAGGCATTGGAGTTGCTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF247

Protein Analysis

458

Amino Acids

53.42

Weight (kDa)

6.04

Isoelectric Point (pI)

51.24

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DUF247	PF03140	22 - 445	6.5e-88	Plant protein of unknown function

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000821)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G65985 AT1G65985 AT1G67150 AT1G67150 AT1G67150 AT1G67150 AT2G28580 AT2G28580 AT2G28580 AT2G28580 AT2G28580 AT2G28580 AT2G44930 AT3G60470
fragaria_vesca	FvH4_7g09691 FvH4_7g09700 FvH4_7g09710
malus_domestica	MD00G1041300.v1.1
prunus_persica	Prupe.2G124600_v2.0.a1 Prupe.2G124800_v2.0.a1 Prupe.2G124900_v2.0.a1 Prupe.2G125100_v2.0.a1 Prupe.2G125200_v2.0.a1 Prupe.2G125200_v2.0.a1 Prupe.2G125200_v2.0.a1 Prupe.2G125400_v2.0.a1 Prupe.2G125400_v2.0.a1
pyrus_communis	pycom02g17910 pycom02g18260 pycom02g18340
rosa_chinensis	RchiOBHm_Chr1g0346611 RchiOBHm_Chr1g0346641 RchiOBHm_Chr1g0346651 RchiOBHm_Chr1g0346791 RchiOBHm_Chr4g0388531
rosa_laevigata	RLG00000010045 RLG00000010060 RLG00000028784 RLG00000028788 RLG00000028789
rosa_multiflora	Rmu_sc0001241.1_g000008 Rmu_sc0001241.1_g000009 Rmu_sc0002357.1_g000013 Rmu_sc0002640.1_g000020 Rmu_sc0005167.1_g000027 Rmu_sc0006463.1_g000009 Rmu_sc0006463.1_g000017
rosa_roxburghii	Rroxscaffold_4G00308200 Rroxscaffold_4G00308310 Rroxscaffold_4G00308390 Rroxscaffold_5G00334860 Rroxscaffold_5G00334990
rosa_rugosa	Rorug01G0181400 Rorug01G0181700 Rorug01G0182300 Rorug03G0318400
rosa_samantha	Rh1AG200700 Rh1BG166900 Rh1BG167000 Rh1BG167700 Rh1DG197800 Rh1DG198500 Rh4BG016600 Rh4DG017100
rosa_wichuraiana	Rw1G016810 Rw1G016820 Rw4G001580 Rw4G001590

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	890, 1236
Acc36I	ACCTGC	1 cut(s)	275
Acc65I	GGTACC	1 cut(s)	287
AccB1I	GGYRCC	1 cut(s)	287
AccB7I	CCANNNNNTGG	1 cut(s)	1258
AciI	CCGC	1 cut(s)	88
AclWI	GGATC	2 cut(s)	261, 1296
AcoI	YGGCCR	1 cut(s)	520
AcuI	CTGAAG	2 cut(s)	863, 1340
AcyI	GRCGYC	1 cut(s)	333
AfaI	GTAC	3 cut(s)	289, 1250, 1306
AfiI	CCNNNNNNNGG	3 cut(s)	778, 1105, 1258
AflIII	ACRYGT	1 cut(s)	559
AluBI	AGCT	8 cut(s)	85, 243, 463, 487, 784, 841, 949, 1310
AluI	AGCT	8 cut(s)	85, 243, 463, 487, 784, 841, 949, 1310
Alw26I	GTCTC	2 cut(s)	531, 1264
AlwI	GGATC	2 cut(s)	261, 1296
AoxI	GGCC	5 cut(s)	134, 174, 520, 611, 1002
ApeKI	GCWGC	3 cut(s)	104, 594, 1134
AseI	ATTAAT	1 cut(s)	1074
Asp700I	GAANNNNTTC	2 cut(s)	267, 651
Asp718I	GGTACC	1 cut(s)	287
AspS9I	GGNCC	4 cut(s)	134, 175, 439, 612
AsuC2I	CCSGG	1 cut(s)	615
AsuHPI	GGTGA	2 cut(s)	402, 629
AsuII	TTCGAA	2 cut(s)	218, 991
AvaII	GGWCC	1 cut(s)	439
BaeI	ACNNNNGTAYC	2 cut(s)	1004, 1037
BalI	TGGCCA	1 cut(s)	522
BanI	GGYRCC	1 cut(s)	287
BbsI	GAAGAC	1 cut(s)	903
BbvCI	CCTCAGC	1 cut(s)	419
BbvI	GCAGC	3 cut(s)	116, 581, 1146
BccI	CCATC	4 cut(s)	244, 531, 644, 1103
BciVI	GTATCC	1 cut(s)	1032
BclI	TGATCA	2 cut(s)	805, 1158
BcnI	CCSGG	1 cut(s)	615
BcoDI	GTCTC	2 cut(s)	531, 1264
BfaI	CTAG	3 cut(s)	326, 1130, 1316
BfmI	CTRYAG	2 cut(s)	67, 1201
BfuAI	ACCTGC	1 cut(s)	275
BfuI	GTATCC	1 cut(s)	1032
BglI	GCCNNNNNGGC	1 cut(s)	528
BglII	AGATCT	1 cut(s)	1209
BisI	GCNGC	3 cut(s)	105, 595, 1135
BlsI	GCNGC	3 cut(s)	106, 596, 1136
Bme1390I	CCNGG	1 cut(s)	615
Bme18I	GGWCC	1 cut(s)	439
BmgT120I	GGNCC	4 cut(s)	134, 175, 439, 612
BmiI	GGNNCC	3 cut(s)	75, 289, 440
BmrFI	CCNGG	1 cut(s)	615
BmrI	ACTGGG	1 cut(s)	914
BmuI	ACTGGG	1 cut(s)	914
BpiI	GAAGAC	1 cut(s)	903
BplI	GAGNNNNNCTC	2 cut(s)	1178, 1210
Bpu10I	CCTNAGC	1 cut(s)	419
Bpu14I	TTCGAA	2 cut(s)	218, 991
BpuMI	CCSGG	1 cut(s)	615
BsaHI	GRCGYC	1 cut(s)	333
BsaI	GGTCTC	1 cut(s)	531
BsaJI	CCNNGG	5 cut(s)	145, 413, 702, 999, 1257
BsaXI	ACNNNNNCTCC	4 cut(s)	773, 803, 1288, 1318
Bsc4I	CCNNNNNNNGG	3 cut(s)	778, 1105, 1258
Bse1I	ACTGG	1 cut(s)	920
Bse3DI	GCAATG	3 cut(s)	97, 538, 596
BseDI	CCNNGG	5 cut(s)	145, 413, 702, 999, 1257
BseGI	GGATG	4 cut(s)	321, 636, 1099, 1114
BseLI	CCNNNNNNNGG	3 cut(s)	778, 1105, 1258
BseMI	GCAATG	3 cut(s)	97, 538, 596
BseMII	CTCAG	5 cut(s)	26, 152, 395, 410, 1071
BseNI	ACTGG	1 cut(s)	920
BseRI	GAGGAG	1 cut(s)	105
BseXI	GCAGC	3 cut(s)	116, 581, 1146
BseYI	CCCAGC	1 cut(s)	77
BshFI	GGCC	5 cut(s)	136, 176, 522, 613, 1004
BshNI	GGYRCC	1 cut(s)	287
BsiSI	CCGG	2 cut(s)	610, 615
BslFI	GGGAC	3 cut(s)	179, 452, 570
BslI	CCNNNNNNNGG	3 cut(s)	778, 1105, 1258
BsmAI	GTCTC	2 cut(s)	531, 1264
BsmFI	GGGAC	3 cut(s)	179, 452, 570
BsmI	GAATGC	2 cut(s)	37, 986
BsnI	GGCC	5 cut(s)	136, 176, 522, 613, 1004
Bso31I	GGTCTC	1 cut(s)	531
Bsp119I	TTCGAA	2 cut(s)	218, 991
Bsp143I	GATC	8 cut(s)	253, 303, 407, 641, 805, 1158, 1209, 1288
Bsp19I	CCATGG	2 cut(s)	145, 999
BspACI	CCGC	1 cut(s)	88
BspANI	GGCC	5 cut(s)	136, 176, 522, 613, 1004
BspCNI	CTCAG	5 cut(s)	25, 153, 396, 411, 1072
BspHI	TCATGA	1 cut(s)	246
BspLI	GGNNCC	3 cut(s)	75, 289, 440
BspMI	ACCTGC	1 cut(s)	275
BspPI	GGATC	2 cut(s)	261, 1296
BspT104I	TTCGAA	2 cut(s)	218, 991
BspT107I	GGYRCC	1 cut(s)	287
BspTNI	GGTCTC	1 cut(s)	531
BsrDI	GCAATG	3 cut(s)	97, 538, 596
BsrI	ACTGG	1 cut(s)	920
BssECI	CCNNGG	5 cut(s)	145, 413, 702, 999, 1257
BssMI	GATC	8 cut(s)	253, 303, 407, 641, 805, 1158, 1209, 1288
BssNI	GRCGYC	1 cut(s)	333
BssT1I	CCWWGG	4 cut(s)	145, 702, 999, 1257
Bst4CI	ACNGT	5 cut(s)	414, 430, 450, 584, 1243
BstACI	GRCGYC	1 cut(s)	333
BstBI	TTCGAA	2 cut(s)	218, 991
BstC8I	GCNNGC	2 cut(s)	83, 337
BstDEI	CTNAG	8 cut(s)	12, 161, 236, 404, 419, 872, 1006, 1080
BstDSI	CCRYGG	3 cut(s)	145, 413, 999
BstENI	CCTNNNNNAGG	1 cut(s)	1103
BstF5I	GGATG	4 cut(s)	321, 636, 1099, 1114
BstKTI	GATC	8 cut(s)	256, 306, 410, 644, 808, 1161, 1212, 1291
BstMAI	GTCTC	2 cut(s)	531, 1264
BstMBI	GATC	8 cut(s)	253, 303, 407, 641, 805, 1158, 1209, 1288
BstMWI	GCNNNNNNNGC	2 cut(s)	87, 528
BstNSI	RCATGY	1 cut(s)	563
BstSCI	CCNGG	1 cut(s)	613
BstSFI	CTRYAG	2 cut(s)	67, 1201
BstV1I	GCAGC	3 cut(s)	116, 581, 1146
BstV2I	GAAGAC	1 cut(s)	903
BstX2I	RGATCY	2 cut(s)	1209, 1288
BstYI	RGATCY	2 cut(s)	1209, 1288
BsuI	GTATCC	1 cut(s)	1032
BsuRI	GGCC	5 cut(s)	136, 176, 522, 613, 1004
BtgI	CCRYGG	3 cut(s)	145, 413, 999
BtsCI	GGATG	4 cut(s)	321, 636, 1099, 1114
BtsI	GCAGTG	1 cut(s)	1152
BtsIMutI	CAGTG	2 cut(s)	744, 1152
BveI	ACCTGC	1 cut(s)	275
Cac8I	GCNNGC	2 cut(s)	83, 337
CciI	TCATGA	1 cut(s)	246
Cfr13I	GGNCC	4 cut(s)	134, 175, 439, 612
CseI	GACGC	1 cut(s)	341
Csp6I	GTAC	3 cut(s)	288, 1249, 1305
CviAII	CATG	8 cut(s)	146, 169, 247, 560, 659, 830, 1000, 1156
CviQI	GTAC	3 cut(s)	288, 1249, 1305
DdeI	CTNAG	8 cut(s)	12, 161, 236, 404, 419, 872, 1006, 1080
DpnI	GATC	8 cut(s)	255, 305, 409, 643, 807, 1160, 1211, 1290
DpnII	GATC	8 cut(s)	253, 303, 407, 641, 805, 1158, 1209, 1288
DraI	TTTAAA	1 cut(s)	295
EaeI	YGGCCR	1 cut(s)	520
Eco130I	CCWWGG	4 cut(s)	145, 702, 999, 1257
Eco31I	GGTCTC	1 cut(s)	531
Eco47I	GGWCC	1 cut(s)	439
Eco57I	CTGAAG	2 cut(s)	863, 1340
EcoNI	CCTNNNNNAGG	1 cut(s)	1103
EcoO109I	RGGNCCY	2 cut(s)	134, 439
EcoT14I	CCWWGG	4 cut(s)	145, 702, 999, 1257
EcoT22I	ATGCAT	1 cut(s)	986
ErhI	CCWWGG	4 cut(s)	145, 702, 999, 1257
FaeI	CATG	8 cut(s)	149, 172, 250, 563, 662, 833, 1003, 1159
FaqI	GGGAC	3 cut(s)	179, 452, 570
FatI	CATG	8 cut(s)	145, 168, 246, 559, 658, 829, 999, 1155
FbaI	TGATCA	2 cut(s)	805, 1158
Fnu4HI	GCNGC	3 cut(s)	105, 595, 1135
FokI	GGATG	4 cut(s)	308, 623, 1106, 1121
Fsp4HI	GCNGC	3 cut(s)	105, 595, 1135
FspBI	CTAG	3 cut(s)	326, 1130, 1316
GluI	GCNGC	3 cut(s)	105, 595, 1135
GsaI	CCCAGC	1 cut(s)	81
HaeIII	GGCC	5 cut(s)	136, 176, 522, 613, 1004
HapII	CCGG	2 cut(s)	610, 615
HgaI	GACGC	1 cut(s)	341
Hin1I	GRCGYC	1 cut(s)	333
Hin1II	CATG	8 cut(s)	149, 172, 250, 563, 662, 833, 1003, 1159
HincII	GTYRAC	2 cut(s)	331, 909
HindII	GTYRAC	2 cut(s)	331, 909
HindIII	AAGCTT	1 cut(s)	947
HinfI	GANTC	2 cut(s)	1063, 1320
HpaII	CCGG	2 cut(s)	610, 615
HphI	GGTGA	2 cut(s)	402, 629
Hpy166II	GTNNAC	5 cut(s)	20, 331, 909, 1153, 1279
Hpy188I	TCNGA	4 cut(s)	15, 370, 641, 646
Hpy188III	TCNNGA	8 cut(s)	247, 260, 626, 668, 718, 1025, 1099, 1217
Hpy8I	GTNNAC	5 cut(s)	20, 331, 909, 1153, 1279
HpyAV	CCTTC	2 cut(s)	838, 1364
HpyCH4III	ACNGT	5 cut(s)	414, 430, 450, 584, 1243
HpyCH4IV	ACGT	2 cut(s)	211, 911
HpyCH4V	TGCA	9 cut(s)	35, 52, 207, 284, 389, 777, 833, 851, 984
HpyF10VI	GCNNNNNNNGC	2 cut(s)	87, 528
HpyF3I	CTNAG	8 cut(s)	12, 161, 236, 404, 419, 872, 1006, 1080
HpySE526I	ACGT	2 cut(s)	211, 911
Hsp92I	GRCGYC	1 cut(s)	333
Hsp92II	CATG	8 cut(s)	149, 172, 250, 563, 662, 833, 1003, 1159
KpnI	GGTACC	1 cut(s)	291
Ksp22I	TGATCA	2 cut(s)	805, 1158
Kzo9I	GATC	8 cut(s)	253, 303, 407, 641, 805, 1158, 1209, 1288
LmnI	GCTCC	3 cut(s)	90, 240, 781
Lsp1109I	GCAGC	3 cut(s)	116, 581, 1146
MaeI	CTAG	3 cut(s)	326, 1130, 1316
MaeII	ACGT	2 cut(s)	211, 911
MalI	GATC	8 cut(s)	255, 305, 409, 643, 807, 1160, 1211, 1290
MboI	GATC	8 cut(s)	253, 303, 407, 641, 805, 1158, 1209, 1288
MboII	GAAGA	7 cut(s)	207, 467, 470, 908, 1097, 1111, 1337
MflI	RGATCY	2 cut(s)	1209, 1288
MlsI	TGGCCA	1 cut(s)	522
MluCI	AATT	9 cut(s)	267, 274, 345, 364, 391, 799, 819, 1332, 1341
MluNI	TGGCCA	1 cut(s)	522
MmeI	TCCRAC	1 cut(s)	432
Mox20I	TGGCCA	1 cut(s)	522
Mph1103I	ATGCAT	1 cut(s)	986
MroXI	GAANNNNTTC	2 cut(s)	267, 651
MscI	TGGCCA	1 cut(s)	522
MseI	TTAA	8 cut(s)	294, 690, 822, 915, 1074, 1182, 1224, 1375
Msp20I	TGGCCA	1 cut(s)	522
MspI	CCGG	2 cut(s)	610, 615
MspR9I	CCNGG	1 cut(s)	615
Mva1269I	GAATGC	2 cut(s)	37, 986
MwoI	GCNNNNNNNGC	2 cut(s)	87, 528
NciI	CCSGG	1 cut(s)	615
NcoI	CCATGG	2 cut(s)	145, 999
NdeII	GATC	8 cut(s)	253, 303, 407, 641, 805, 1158, 1209, 1288
NlaIII	CATG	8 cut(s)	149, 172, 250, 563, 662, 833, 1003, 1159
NlaIV	GGNNCC	3 cut(s)	75, 289, 440
NsiI	ATGCAT	1 cut(s)	986
NspI	RCATGY	1 cut(s)	563
NspV	TTCGAA	2 cut(s)	218, 991
PagI	TCATGA	1 cut(s)	246
PciI	ACATGT	1 cut(s)	559
PctI	GAATGC	2 cut(s)	37, 986
PdmI	GAANNNNTTC	2 cut(s)	267, 651
PfeI	GAWTC	2 cut(s)	1063, 1320
PflMI	CCANNNNNTGG	1 cut(s)	1258
PkrI	GCNGC	3 cut(s)	106, 596, 1136
PpuMI	RGGWCCY	1 cut(s)	439
PscI	ACATGT	1 cut(s)	559
PshBI	ATTAAT	1 cut(s)	1074
PsiI	TTATAA	2 cut(s)	890, 1236
Psp5II	RGGWCCY	1 cut(s)	439
PspFI	CCCAGC	1 cut(s)	77
PspN4I	GGNNCC	3 cut(s)	75, 289, 440
PspPI	GGNCC	4 cut(s)	134, 175, 439, 612
PspPPI	RGGWCCY	1 cut(s)	439
PsuI	RGATCY	2 cut(s)	1209, 1288
RsaI	GTAC	3 cut(s)	289, 1250, 1306
RsaNI	GTAC	3 cut(s)	288, 1249, 1305
SaqAI	TTAA	8 cut(s)	294, 690, 822, 915, 1074, 1182, 1224, 1375
SatI	GCNGC	3 cut(s)	105, 595, 1135
Sau3AI	GATC	8 cut(s)	253, 303, 407, 641, 805, 1158, 1209, 1288
Sau96I	GGNCC	4 cut(s)	134, 175, 439, 612
ScrFI	CCNGG	1 cut(s)	615
SfcI	CTRYAG	2 cut(s)	67, 1201
SfuI	TTCGAA	2 cut(s)	218, 991
SinI	GGWCC	1 cut(s)	439
Sse9I	AATT	9 cut(s)	267, 274, 345, 364, 391, 799, 819, 1332, 1341
SsiI	CCGC	1 cut(s)	88
SspI	AATATT	1 cut(s)	191
SspMI	CTAG	3 cut(s)	326, 1130, 1316
StyD4I	CCNGG	1 cut(s)	613
StyI	CCWWGG	4 cut(s)	145, 702, 999, 1257
TaaI	ACNGT	5 cut(s)	414, 430, 450, 584, 1243
TaiI	ACGT	2 cut(s)	214, 914
TaqI	TCGA	4 cut(s)	218, 259, 306, 991
TasI	AATT	9 cut(s)	267, 274, 345, 364, 391, 799, 819, 1332, 1341
TatI	WGTACW	1 cut(s)	1304
TfiI	GAWTC	2 cut(s)	1063, 1320
Tru1I	TTAA	8 cut(s)	294, 690, 822, 915, 1074, 1182, 1224, 1375
Tru9I	TTAA	8 cut(s)	294, 690, 822, 915, 1074, 1182, 1224, 1375
TscAI	CASTG	2 cut(s)	751, 1152
TseI	GCWGC	3 cut(s)	104, 594, 1134
TspDTI	ATGAA	5 cut(s)	114, 235, 331, 1076, 1155
TspRI	CASTG	2 cut(s)	751, 1152
Van91I	CCANNNNNTGG	1 cut(s)	1258
VpaK11BI	GGWCC	1 cut(s)	439
VspI	ATTAAT	1 cut(s)	1074
XagI	CCTNNNNNAGG	1 cut(s)	1103
XceI	RCATGY	1 cut(s)	563
XcmI	CCANNNNNNNNNTGG	2 cut(s)	530, 548
XmnI	GAANNNNTTC	2 cut(s)	267, 651
XspI	CTAG	3 cut(s)	326, 1130, 1316
Zsp2I	ATGCAT	1 cut(s)	986

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.