AT1G68440

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0001093
Physical Location & Seq
Forward (+)
25657757 .. 25659484
1728 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G68440.1

Sequence Viewer

Length: 921 bp
ATGGAGGTACCGGTGATTAATAGAATAAGAGATTTCGAAGTTGGTATAAACTCGATTAATGATCCTTCGTTTCTTTCTCGATCTGTTGCTGTTTCCGGAATCGGAAAGCTACACCAAGCTTATGGTTTTTGGAAATGGGGAGCTTTGATTATCGCATTTCTAGCTTATTTCACCAACTTTGTTAGTAAACTCAACAGTCTAGTTGTTAGGTTAAGAAAAATAGATGTCTCTGTTTCCTCTCCAACTCTTTTTGATGATTACGATAGCGATTCCGATGTTTCTTGTTCCTCTACCGTCTCCTCTGACGATGAAAAAGACGAAGAAGATGAGGCTGATGATGAAGATGAGGATGTTGACTCGATCTTTAATCGAAGACGAGTCAACGGAGGTTTCCGCGTTAGAGGATCTGATTATTACGATGATGATGATGATCAGGGAGACAATGGTAATTGCACATGGATGGGGAGGCGATATAGTGGTAGTTTTGGAGATTTGTTCTCATGGCCTGATCTTGGTGGGATTGGTTCGAGTGGAGTCGTGAAGCTTTGGGATCATTTAGATATCGATGGCGATGATCATGAGAATGTTGTGGCAACGTTTCTCAAGAACTATAACTCAACCTCGTCACCGTTTTTTTGGGCGGCGGAGAAAAAAGGTGTTGACGCCGTTAAAGTGAAGGCGTGTGATCCACGCGCCGGTTTTCGGATGCCGGCTTTGCTCGCGGAGTGGAGGCAGCCGGGGCGGTTGTTAGGGAATATAATCGGAGTTGATACCGGTGGTGTGGAGAAAGTATACGTCAGGGATGATGTTAGCGGAGAGATAGCCGTGGGAGATTTGAGGAAGTTTAACGGTGTGTTGACGGACTTGACGGAATGTGAGGCTGAGACTTGGTGGGACGCTGACGTCCTTATCTCGGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

306

Amino Acids

33.92

Weight (kDa)

4.36

Isoelectric Point (pI)

34.77

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 902
AatII GACGTC 1 cut(s) 906
Acc65I GGTACC 1 cut(s) 7
AccB1I GGYRCC 1 cut(s) 7
AccI GTMKAC 1 cut(s) 792
AccII CGCG 3 cut(s) 396, 693, 722
AccIII TCCGGA 1 cut(s) 95
AciI CCGC 6 cut(s) 394, 641, 644, 722, 742, 813
AclI AACGTT 1 cut(s) 596
AclWI GGATC 4 cut(s) 56, 412, 558, 680
AcyI GRCGYC 2 cut(s) 663, 903
AfaI GTAC 1 cut(s) 9
AfiI CCNNNNNNNGG 4 cut(s) 512, 695, 702, 913
AgeI ACCGGT 2 cut(s) 10, 773
AluBI AGCT 5 cut(s) 109, 119, 143, 164, 544
AluI AGCT 5 cut(s) 109, 119, 143, 164, 544
Alw26I GTCTC 4 cut(s) 232, 301, 432, 878
AlwI GGATC 4 cut(s) 56, 412, 558, 680
Ama87I CYCGRG 1 cut(s) 913
Aor13HI TCCGGA 1 cut(s) 95
AoxI GGCC 1 cut(s) 503
ApeKI GCWGC 1 cut(s) 733
AseI ATTAAT 2 cut(s) 18, 57
AsiGI ACCGGT 2 cut(s) 10, 773
Asp718I GGTACC 1 cut(s) 7
AspLEI GCGC 1 cut(s) 695
AsuC2I CCSGG 1 cut(s) 738
AsuHPI GGTGA 3 cut(s) 25, 163, 618
AsuII TTCGAA 1 cut(s) 36
AvaI CYCGRG 1 cut(s) 913
BanI GGYRCC 1 cut(s) 7
BbsI GAAGAC 1 cut(s) 379
BbvI GCAGC 1 cut(s) 745
BccI CCATC 2 cut(s) 454, 560
BceAI ACGGC 2 cut(s) 650, 809
BclI TGATCA 2 cut(s) 430, 574
BcnI CCSGG 1 cut(s) 738
BcoDI GTCTC 4 cut(s) 232, 301, 432, 878
BfaI CTAG 2 cut(s) 161, 200
BisI GCNGC 2 cut(s) 642, 734
BlsI GCNGC 2 cut(s) 643, 735
Bme1390I CCNGG 1 cut(s) 738
BmeT110I CYCGRG 1 cut(s) 913
BmiI GGNNCC 1 cut(s) 9
BmrFI CCNGG 1 cut(s) 738
BmsI GCATC 1 cut(s) 696
BpiI GAAGAC 1 cut(s) 379
Bpu14I TTCGAA 1 cut(s) 36
BpuEI CTTGAG 1 cut(s) 587
BpuMI CCSGG 1 cut(s) 738
Bsa29I ATCGAT 1 cut(s) 564
BsaBI GATNNNNATC 1 cut(s) 429
BsaHI GRCGYC 2 cut(s) 663, 903
BsaJI CCNNGG 2 cut(s) 737, 825
BsaWI WCCGGW 3 cut(s) 10, 95, 773
Bsc4I CCNNNNNNNGG 4 cut(s) 512, 695, 702, 913
Bse118I RCCGGY 4 cut(s) 10, 695, 709, 773
Bse8I GATNNNNATC 1 cut(s) 429
BseAI TCCGGA 1 cut(s) 95
BseCI ATCGAT 1 cut(s) 564
BseDI CCNNGG 2 cut(s) 737, 825
BseGI GGATG 4 cut(s) 355, 465, 711, 808
BseJI GATNNNNATC 1 cut(s) 429
BseLI CCNNNNNNNGG 4 cut(s) 512, 695, 702, 913
BseMII CTCAG 1 cut(s) 873
BseRI GAGGAG 1 cut(s) 289
BseXI GCAGC 1 cut(s) 745
Bsh1236I CGCG 3 cut(s) 396, 693, 722
BshFI GGCC 1 cut(s) 505
BshNI GGYRCC 1 cut(s) 7
BshTI ACCGGT 2 cut(s) 10, 773
BshVI ATCGAT 1 cut(s) 564
BsiHKCI CYCGRG 1 cut(s) 913
BsiSI CCGG 6 cut(s) 11, 96, 696, 710, 737, 774
BslFI GGGAC 1 cut(s) 908
BslI CCNNNNNNNGG 4 cut(s) 512, 695, 702, 913
BsmAI GTCTC 4 cut(s) 232, 301, 432, 878
BsmBI CGTCTC 1 cut(s) 301
BsmFI GGGAC 1 cut(s) 908
BsnI GGCC 1 cut(s) 505
BsoBI CYCGRG 1 cut(s) 913
Bsp119I TTCGAA 1 cut(s) 36
Bsp13I TCCGGA 1 cut(s) 95
Bsp143I GATC 9 cut(s) 61, 80, 360, 404, 430, 508, 550, 574, 685
BspACI CCGC 6 cut(s) 394, 641, 644, 722, 742, 813
BspANI GGCC 1 cut(s) 505
BspCNI CTCAG 1 cut(s) 874
BspDI ATCGAT 1 cut(s) 564
BspEI TCCGGA 1 cut(s) 95
BspFNI CGCG 3 cut(s) 396, 693, 722
BspHI TCATGA 1 cut(s) 577
BspLI GGNNCC 1 cut(s) 9
BspPI GGATC 4 cut(s) 56, 412, 558, 680
BspT104I TTCGAA 1 cut(s) 36
BspT107I GGYRCC 1 cut(s) 7
BsrFI RCCGGY 4 cut(s) 10, 695, 709, 773
BssAI RCCGGY 4 cut(s) 10, 695, 709, 773
BssECI CCNNGG 2 cut(s) 737, 825
BssMI GATC 9 cut(s) 61, 80, 360, 404, 430, 508, 550, 574, 685
BssNAI GTATAC 1 cut(s) 793
BssNI GRCGYC 2 cut(s) 663, 903
Bst1107I GTATAC 1 cut(s) 793
Bst4CI ACNGT 4 cut(s) 197, 295, 630, 851
BstACI GRCGYC 2 cut(s) 663, 903
BstBI TTCGAA 1 cut(s) 36
BstC8I GCNNGC 2 cut(s) 711, 720
BstDEI CTNAG 1 cut(s) 882
BstDSI CCRYGG 1 cut(s) 825
BstF5I GGATG 4 cut(s) 355, 465, 711, 808
BstFNI CGCG 3 cut(s) 396, 693, 722
BstHHI GCGC 1 cut(s) 695
BstKTI GATC 9 cut(s) 64, 83, 363, 407, 433, 511, 553, 577, 688
BstMAI GTCTC 4 cut(s) 232, 301, 432, 878
BstMBI GATC 9 cut(s) 61, 80, 360, 404, 430, 508, 550, 574, 685
BstMWI GCNNNNNNNGC 4 cut(s) 161, 715, 719, 739
BstSCI CCNGG 1 cut(s) 736
BstUI CGCG 3 cut(s) 396, 693, 722
BstV1I GCAGC 1 cut(s) 745
BstV2I GAAGAC 1 cut(s) 379
BstX2I RGATCY 1 cut(s) 404
BstXI CCANNNNNNTGG 1 cut(s) 122
BstYI RGATCY 1 cut(s) 404
BstZ17I GTATAC 1 cut(s) 793
Bsu15I ATCGAT 1 cut(s) 564
BsuRI GGCC 1 cut(s) 505
BsuTUI ATCGAT 1 cut(s) 564
BtgI CCRYGG 1 cut(s) 825
BtgZI GCGATG 1 cut(s) 585
BtsCI GGATG 4 cut(s) 355, 465, 711, 808
Cac8I GCNNGC 2 cut(s) 711, 720
CciI TCATGA 1 cut(s) 577
CfoI GCGC 1 cut(s) 695
Cfr10I RCCGGY 4 cut(s) 10, 695, 709, 773
ClaI ATCGAT 1 cut(s) 564
CseI GACGC 2 cut(s) 671, 905
Csp6I GTAC 1 cut(s) 8
CspAI ACCGGT 2 cut(s) 10, 773
CviAII CATG 3 cut(s) 456, 501, 578
CviQI GTAC 1 cut(s) 8
DdeI CTNAG 1 cut(s) 882
DpnI GATC 9 cut(s) 63, 82, 362, 406, 432, 510, 552, 576, 687
DpnII GATC 9 cut(s) 61, 80, 360, 404, 430, 508, 550, 574, 685
DrdI GACNNNNNNGTC 1 cut(s) 902
DseDI GACNNNNNNGTC 1 cut(s) 902
EciI GGCGGA 1 cut(s) 659
Eco32I GATATC 1 cut(s) 562
Eco88I CYCGRG 1 cut(s) 913
EcoRV GATATC 1 cut(s) 562
Esp3I CGTCTC 1 cut(s) 301
FaeI CATG 3 cut(s) 459, 504, 581
FaiI YATR 9 cut(s) 47, 123, 457, 474, 502, 579, 612, 758, 793
FaqI GGGAC 1 cut(s) 908
FatI CATG 3 cut(s) 455, 500, 577
FbaI TGATCA 2 cut(s) 430, 574
FblI GTMKAC 1 cut(s) 792
Fnu4HI GCNGC 2 cut(s) 642, 734
FokI GGATG 4 cut(s) 362, 472, 718, 815
Fsp4HI GCNGC 2 cut(s) 642, 734
FspBI CTAG 2 cut(s) 161, 200
GlaI GCGC 1 cut(s) 694
GluI GCNGC 2 cut(s) 642, 734
HaeIII GGCC 1 cut(s) 505
HapII CCGG 6 cut(s) 11, 96, 696, 710, 737, 774
HgaI GACGC 2 cut(s) 671, 905
HhaI GCGC 1 cut(s) 695
Hin1I GRCGYC 2 cut(s) 663, 903
Hin1II CATG 3 cut(s) 459, 504, 581
Hin6I GCGC 1 cut(s) 693
HinP1I GCGC 1 cut(s) 693
HincII GTYRAC 4 cut(s) 355, 382, 661, 858
HindII GTYRAC 4 cut(s) 355, 382, 661, 858
HindIII AAGCTT 2 cut(s) 117, 542
HinfI GANTC 5 cut(s) 99, 269, 356, 378, 534
HpaII CCGG 6 cut(s) 11, 96, 696, 710, 737, 774
HphI GGTGA 3 cut(s) 25, 163, 618
Hpy166II GTNNAC 6 cut(s) 188, 355, 382, 661, 793, 858
Hpy188I TCNGA 6 cut(s) 104, 274, 304, 409, 705, 764
Hpy188III TCNNGA 5 cut(s) 78, 96, 538, 578, 604
Hpy8I GTNNAC 6 cut(s) 188, 355, 382, 661, 793, 858
HpyAV CCTTC 2 cut(s) 75, 670
HpyCH4III ACNGT 4 cut(s) 197, 295, 630, 851
HpyCH4IV ACGT 3 cut(s) 596, 795, 903
HpyCH4V TGCA 1 cut(s) 453
HpyF10VI GCNNNNNNNGC 4 cut(s) 161, 715, 719, 739
HpyF3I CTNAG 1 cut(s) 882
HpySE526I ACGT 3 cut(s) 596, 795, 903
Hsp92I GRCGYC 2 cut(s) 663, 903
Hsp92II CATG 3 cut(s) 459, 504, 581
HspAI GCGC 1 cut(s) 693
Kpn2I TCCGGA 1 cut(s) 95
KpnI GGTACC 1 cut(s) 11
KroI GCCGGC 1 cut(s) 709
KroNI GCCGGC 1 cut(s) 711
Ksp22I TGATCA 2 cut(s) 430, 574
Kzo9I GATC 9 cut(s) 61, 80, 360, 404, 430, 508, 550, 574, 685
LmnI GCTCC 1 cut(s) 140
LpnPI CCDG 9 cut(s) 24, 109, 419, 519, 709, 723, 750, 784, 787
Lsp1109I GCAGC 1 cut(s) 745
LweI GCATC 1 cut(s) 696
MaeI CTAG 2 cut(s) 161, 200
MaeII ACGT 3 cut(s) 596, 795, 903
MaeIII GTNAC 1 cut(s) 624
MalI GATC 9 cut(s) 63, 82, 362, 406, 432, 510, 552, 576, 687
MboI GATC 9 cut(s) 61, 80, 360, 404, 430, 508, 550, 574, 685
MboII GAAGA 4 cut(s) 332, 335, 353, 384
MflI RGATCY 1 cut(s) 404
MluCI AATT 1 cut(s) 448
MlyI GAGTC 3 cut(s) 350, 387, 543
MmeI TCCRAC 1 cut(s) 266
MroI TCCGGA 1 cut(s) 95
MroNI GCCGGC 1 cut(s) 709
MseI TTAA 6 cut(s) 18, 57, 212, 366, 669, 846
MslI CAYNNNNRTG 2 cut(s) 458, 582
MspI CCGG 6 cut(s) 11, 96, 696, 710, 737, 774
MspR9I CCNGG 1 cut(s) 738
MvnI CGCG 3 cut(s) 396, 693, 722
MwoI GCNNNNNNNGC 4 cut(s) 161, 715, 719, 739
NaeI GCCGGC 1 cut(s) 711
NciI CCSGG 1 cut(s) 738
NdeII GATC 9 cut(s) 61, 80, 360, 404, 430, 508, 550, 574, 685
NgoMIV GCCGGC 1 cut(s) 709
NlaIII CATG 3 cut(s) 459, 504, 581
NlaIV GGNNCC 1 cut(s) 9
NmuCI GTSAC 1 cut(s) 624
NspV TTCGAA 1 cut(s) 36
PagI TCATGA 1 cut(s) 577
PdiI GCCGGC 1 cut(s) 711
PfeI GAWTC 2 cut(s) 99, 269
PinAI ACCGGT 2 cut(s) 10, 773
PkrI GCNGC 2 cut(s) 643, 735
PleI GAGTC 3 cut(s) 350, 386, 542
PpsI GAGTC 3 cut(s) 350, 386, 542
PshBI ATTAAT 2 cut(s) 18, 57
Psp1406I AACGTT 1 cut(s) 596
PspN4I GGNNCC 1 cut(s) 9
PsuI RGATCY 1 cut(s) 404
RsaI GTAC 1 cut(s) 9
RsaNI GTAC 1 cut(s) 8
RseI CAYNNNNRTG 2 cut(s) 458, 582
SaqAI TTAA 6 cut(s) 18, 57, 212, 366, 669, 846
SatI GCNGC 2 cut(s) 642, 734
Sau3AI GATC 9 cut(s) 61, 80, 360, 404, 430, 508, 550, 574, 685
SchI GAGTC 3 cut(s) 350, 387, 543
ScrFI CCNGG 1 cut(s) 738
SfaNI GCATC 1 cut(s) 696
SfuI TTCGAA 1 cut(s) 36
SmiMI CAYNNNNRTG 2 cut(s) 458, 582
SmlI CTYRAG 1 cut(s) 602
SmoI CTYRAG 1 cut(s) 602
Sse9I AATT 1 cut(s) 448
SsiI CCGC 6 cut(s) 394, 641, 644, 722, 742, 813
SspMI CTAG 2 cut(s) 161, 200
StyD4I CCNGG 1 cut(s) 736
TaaI ACNGT 4 cut(s) 197, 295, 630, 851
TaiI ACGT 3 cut(s) 599, 798, 906
TaqI TCGA 7 cut(s) 36, 53, 79, 359, 370, 527, 564
TasI AATT 1 cut(s) 448
TauI GCSGC 1 cut(s) 644
TfiI GAWTC 2 cut(s) 99, 269
Tru1I TTAA 6 cut(s) 18, 57, 212, 366, 669, 846
Tru9I TTAA 6 cut(s) 18, 57, 212, 366, 669, 846
TseFI GTSAC 1 cut(s) 624
TseI GCWGC 1 cut(s) 733
Tsp45I GTSAC 1 cut(s) 624
TspDTI ATGAA 2 cut(s) 324, 354
TspGWI ACGGA 3 cut(s) 399, 875, 884
VspI ATTAAT 2 cut(s) 18, 57
XmiI GTMKAC 1 cut(s) 792
XspI CTAG 2 cut(s) 161, 200
ZraI GACGTC 1 cut(s) 904
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.