Rorug04G0169800

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0001093
Physical Location & Seq
Forward (+)
29911507 .. 29912115
609 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0169800.1

Sequence Viewer

Length: 510 bp
ATGAAGAATACCGAGCTATGCCATAGCCGCCGACCTCAGAAGCAACATGAGAAGGAAGACGACGATGATCAGAAACCCTACCTTGTACAATTGCCAGACCATATAATTGCCGATATCTCCTGCAAAATCCCAATCAAGACCCTCATGAAATGCAGGCATGTCTGCAAATCTTTGCGATCTTTGTTCGCAGACCCGCAATTCACCGAAGACCTATTCTCTCGAACACGCCCTTGCCATTTGGTCTCGAGCCTCAATTCGGATAGGTACTTCTTGGTCGACTTCAACGAGCGGAGGCCAAACGGCGTCGCGGCACTGGATCTTTCTATAGACTCAGCGACTCCTAGCAGCGCAATCGTGGTGGGATCTTGCAAGGGCTTGGTCTGCTTATACAAGATGTACTGCCCTTACTGTTTTTACATATGCAATCCAATGACTGGTGATTCCAGTTTCCTTTATTGGCCTTTATCTCTGGAGGAGGAGTTAGCTCCTGTAGAGCAAGGGCGTAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

169

Amino Acids

19.33

Weight (kDa)

6.19

Isoelectric Point (pI)

60.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 30 - 68 2e-07 F-box domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 434
AccBSI CCGCTC 1 cut(s) 289
AccI GTMKAC 1 cut(s) 276
AccII CGCG 1 cut(s) 308
AciI CCGC 4 cut(s) 28, 194, 289, 308
AclWI GGATC 2 cut(s) 324, 370
AcyI GRCGYC 1 cut(s) 303
AfaI GTAC 3 cut(s) 87, 266, 398
AfiI CCNNNNNNNGG 2 cut(s) 256, 434
AgsI TTSAA 1 cut(s) 283
AluBI AGCT 3 cut(s) 16, 485, 507
AluI AGCT 3 cut(s) 16, 485, 507
Alw26I GTCTC 1 cut(s) 247
AlwI GGATC 2 cut(s) 324, 370
Ama87I CYCGRG 1 cut(s) 244
AoxI GGCC 2 cut(s) 293, 458
ApeKI GCWGC 1 cut(s) 345
AspLEI GCGC 1 cut(s) 350
AsuHPI GGTGA 2 cut(s) 193, 449
AvaI CYCGRG 1 cut(s) 244
BbsI GAAGAC 2 cut(s) 63, 213
BbvI GCAGC 1 cut(s) 357
BceAI ACGGC 1 cut(s) 316
BcgI CGANNNNNNTGC 2 cut(s) 334, 368
BclI TGATCA 1 cut(s) 67
BcoDI GTCTC 1 cut(s) 247
BfaI CTAG 2 cut(s) 342, 508
BfmI CTRYAG 2 cut(s) 324, 489
BisI GCNGC 3 cut(s) 28, 309, 346
BlsI GCNGC 3 cut(s) 29, 310, 347
BmeT110I CYCGRG 1 cut(s) 244
BpiI GAAGAC 2 cut(s) 63, 213
BpmI CTGGAG 1 cut(s) 491
BsaHI GRCGYC 1 cut(s) 303
BsaI GGTCTC 1 cut(s) 247
BsaXI ACNNNNNCTCC 2 cut(s) 464, 494
Bsc4I CCNNNNNNNGG 2 cut(s) 256, 434
Bse1I ACTGG 3 cut(s) 318, 439, 444
BseLI CCNNNNNNNGG 2 cut(s) 256, 434
BseMII CTCAG 2 cut(s) 50, 345
BseNI ACTGG 3 cut(s) 318, 439, 444
BseRI GAGGAG 2 cut(s) 488, 491
BseXI GCAGC 1 cut(s) 357
Bsh1236I CGCG 1 cut(s) 308
BshFI GGCC 2 cut(s) 295, 460
BsiHKCI CYCGRG 1 cut(s) 244
BslI CCNNNNNNNGG 2 cut(s) 256, 434
BsmAI GTCTC 1 cut(s) 247
BsnI GGCC 2 cut(s) 295, 460
Bso31I GGTCTC 1 cut(s) 247
BsoBI CYCGRG 1 cut(s) 244
Bsp1407I TGTACA 1 cut(s) 85
Bsp143I GATC 4 cut(s) 67, 176, 316, 362
BspACI CCGC 4 cut(s) 28, 194, 289, 308
BspANI GGCC 2 cut(s) 295, 460
BspCNI CTCAG 2 cut(s) 49, 344
BspFNI CGCG 1 cut(s) 308
BspHI TCATGA 1 cut(s) 144
BspPI GGATC 2 cut(s) 324, 370
BspTNI GGTCTC 1 cut(s) 247
BsrBI CCGCTC 1 cut(s) 289
BsrGI TGTACA 1 cut(s) 85
BsrI ACTGG 3 cut(s) 318, 439, 444
BssMI GATC 4 cut(s) 67, 176, 316, 362
BssNI GRCGYC 1 cut(s) 303
Bst4CI ACNGT 1 cut(s) 410
BstACI GRCGYC 1 cut(s) 303
BstAUI TGTACA 1 cut(s) 85
BstC8I GCNNGC 1 cut(s) 155
BstDEI CTNAG 2 cut(s) 36, 331
BstFNI CGCG 1 cut(s) 308
BstHHI GCGC 1 cut(s) 350
BstKTI GATC 4 cut(s) 70, 179, 319, 365
BstMAI GTCTC 1 cut(s) 247
BstMBI GATC 4 cut(s) 67, 176, 316, 362
BstMWI GCNNNNNNNGC 2 cut(s) 27, 381
BstNSI RCATGY 1 cut(s) 161
BstSFI CTRYAG 2 cut(s) 324, 489
BstUI CGCG 1 cut(s) 308
BstV1I GCAGC 1 cut(s) 357
BstV2I GAAGAC 2 cut(s) 63, 213
BstX2I RGATCY 2 cut(s) 316, 362
BstYI RGATCY 2 cut(s) 316, 362
BsuRI GGCC 2 cut(s) 295, 460
BtsIMutI CAGTG 1 cut(s) 311
Cac8I GCNNGC 1 cut(s) 155
CciI TCATGA 1 cut(s) 144
CfoI GCGC 1 cut(s) 350
CseI GACGC 1 cut(s) 292
Csp6I GTAC 3 cut(s) 86, 265, 397
CspCI CAANNNNNGTGG 2 cut(s) 339, 374
CviAII CATG 3 cut(s) 47, 145, 158
CviJI RGCY 8 cut(s) 16, 27, 249, 295, 375, 460, 485, 507
CviKI_1 RGCY 8 cut(s) 16, 27, 249, 295, 375, 460, 485, 507
CviQI GTAC 3 cut(s) 86, 265, 397
DdeI CTNAG 2 cut(s) 36, 331
DpnI GATC 4 cut(s) 69, 178, 318, 364
DpnII GATC 4 cut(s) 67, 176, 316, 362
Eco31I GGTCTC 1 cut(s) 247
Eco32I GATATC 1 cut(s) 115
Eco88I CYCGRG 1 cut(s) 244
EcoRV GATATC 1 cut(s) 115
FaeI CATG 3 cut(s) 50, 148, 161
FatI CATG 3 cut(s) 46, 144, 157
FauI CCCGC 1 cut(s) 201
FauNDI CATATG 1 cut(s) 419
FbaI TGATCA 1 cut(s) 67
FblI GTMKAC 1 cut(s) 276
Fnu4HI GCNGC 3 cut(s) 28, 309, 346
Fsp4HI GCNGC 3 cut(s) 28, 309, 346
FspBI CTAG 2 cut(s) 342, 508
GlaI GCGC 1 cut(s) 349
GluI GCNGC 3 cut(s) 28, 309, 346
GsuI CTGGAG 1 cut(s) 491
HaeIII GGCC 2 cut(s) 295, 460
HgaI GACGC 1 cut(s) 292
HhaI GCGC 1 cut(s) 350
Hin1I GRCGYC 1 cut(s) 303
Hin1II CATG 3 cut(s) 50, 148, 161
Hin6I GCGC 1 cut(s) 348
HinP1I GCGC 1 cut(s) 348
HincII GTYRAC 1 cut(s) 277
HindII GTYRAC 1 cut(s) 277
HinfI GANTC 3 cut(s) 329, 337, 440
HphI GGTGA 2 cut(s) 193, 449
Hpy166II GTNNAC 1 cut(s) 277
Hpy188I TCNGA 3 cut(s) 39, 72, 259
Hpy188III TCNNGA 5 cut(s) 136, 145, 219, 244, 470
Hpy8I GTNNAC 1 cut(s) 277
Hpy99I CGWCG 2 cut(s) 65, 308
HpyAV CCTTC 1 cut(s) 46
HpyCH4III ACNGT 1 cut(s) 410
HpyCH4V TGCA 5 cut(s) 123, 153, 165, 369, 423
HpyF10VI GCNNNNNNNGC 2 cut(s) 27, 381
HpyF3I CTNAG 2 cut(s) 36, 331
Hsp92I GRCGYC 1 cut(s) 303
Hsp92II CATG 3 cut(s) 50, 148, 161
HspAI GCGC 1 cut(s) 348
Ksp22I TGATCA 1 cut(s) 67
Kzo9I GATC 4 cut(s) 67, 176, 316, 362
LmnI GCTCC 1 cut(s) 490
LpnPI CCDG 8 cut(s) 108, 133, 139, 299, 420, 455, 457, 501
Lsp1109I GCAGC 1 cut(s) 357
MaeI CTAG 2 cut(s) 342, 508
MalI GATC 4 cut(s) 69, 178, 318, 364
MbiI CCGCTC 1 cut(s) 289
MboI GATC 4 cut(s) 67, 176, 316, 362
MboII GAAGA 3 cut(s) 16, 68, 218
MfeI CAATTG 1 cut(s) 89
MflI RGATCY 2 cut(s) 316, 362
MluCI AATT 4 cut(s) 89, 105, 197, 253
MlyI GAGTC 2 cut(s) 323, 331
MnlI CCTC 6 cut(s) 45, 152, 260, 285, 466, 469
MunI CAATTG 1 cut(s) 89
MvnI CGCG 1 cut(s) 308
MwoI GCNNNNNNNGC 2 cut(s) 27, 381
NdeI CATATG 1 cut(s) 419
NdeII GATC 4 cut(s) 67, 176, 316, 362
NlaIII CATG 3 cut(s) 50, 148, 161
NspI RCATGY 1 cut(s) 161
PaeR7I CTCGAG 1 cut(s) 244
PagI TCATGA 1 cut(s) 144
PcsI WCGNNNNNNNCGW 1 cut(s) 282
PfeI GAWTC 1 cut(s) 440
PflMI CCANNNNNTGG 1 cut(s) 434
PkrI GCNGC 3 cut(s) 29, 310, 347
PleI GAGTC 2 cut(s) 323, 331
PpsI GAGTC 2 cut(s) 323, 331
PsuI RGATCY 2 cut(s) 316, 362
RsaI GTAC 3 cut(s) 87, 266, 398
RsaNI GTAC 3 cut(s) 86, 265, 397
SalI GTCGAC 1 cut(s) 275
SatI GCNGC 3 cut(s) 28, 309, 346
Sau3AI GATC 4 cut(s) 67, 176, 316, 362
SchI GAGTC 2 cut(s) 323, 331
SetI ASST 7 cut(s) 18, 37, 84, 213, 266, 487, 509
SfcI CTRYAG 2 cut(s) 324, 489
Sfr274I CTCGAG 1 cut(s) 244
SlaI CTCGAG 1 cut(s) 244
SmlI CTYRAG 1 cut(s) 244
SmoI CTYRAG 1 cut(s) 244
Sse9I AATT 4 cut(s) 89, 105, 197, 253
SsiI CCGC 4 cut(s) 28, 194, 289, 308
SspMI CTAG 2 cut(s) 342, 508
TaaI ACNGT 1 cut(s) 410
TaqI TCGA 3 cut(s) 220, 245, 276
TasI AATT 4 cut(s) 89, 105, 197, 253
TatI WGTACW 2 cut(s) 85, 396
TauI GCSGC 2 cut(s) 30, 311
TfiI GAWTC 1 cut(s) 440
TscAI CASTG 1 cut(s) 318
TseI GCWGC 1 cut(s) 345
TspDTI ATGAA 2 cut(s) 17, 161
TspRI CASTG 1 cut(s) 318
Van91I CCANNNNNTGG 1 cut(s) 434
XceI RCATGY 1 cut(s) 161
XhoI CTCGAG 1 cut(s) 244
XmiI GTMKAC 1 cut(s) 276
XspI CTAG 2 cut(s) 342, 508
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.