Rorug04G0021800

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0001093
Physical Location & Seq
Forward (+)
3122425 .. 3123997
1573 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0021800.1

Sequence Viewer

Length: 1392 bp
ATGGATTTCAGAATGGATGCTAGAGAAATAGATAGAAATAAAGAATTATTTGAGAGAAAGGGAAGGCTACATGCGCCTGTTATTCATGATGGGAGAACGACTGTGACAATTTCAGAGGCAGGGTACCAATACGGATTAGAAGAATGCAAACACATGTTATTGGGTCGTCTCTATATGGCAGCCAAGGATAAACCCTATTCTCCATCAGACTTGTATCGTAAGTTGGGATTGGTTTGGGGTGATATTGGCCATTGGAGAATTATTCCCATGGGCAAGGGATACTTCTCTTTCAGCTTTACATCTGATGCGGCTTGTTCTAGGGTTTGGGAGAAGGGTGCAATTGCCCTTAAGCCTGGGATACTGAGGTTTATGAGGTGGGCACCCAATTTTCCCTTGCAAATCAAAAGAACACCAATGCTCAGGGTCTTGGTTGATATTGATTTATCTTCTGATCCTCCACATGAATTGGCTGTTCGTCGCAACAACGGAGATATTGTTGTGATTGAAGTTGGATACAAGCGTCTCCCTGATCTTTGCTCGCATTGTGGCAATGTTGGGCACAACGTTACAACTTGTAAGCTTCTACGAAATCATGCAGCTTCTGGTCAGACTGTGATTGAGATGGGTCAAGGTCAATCGTGTAAGCGCCGTCGACGGAAGACAAAACAAGTATTTGTTGCAAAGAATACTGAGCAAGTGGATAAGGGAAAGAAGATGGTTGTTGCTCCTATGCCAAGTGTGAATGCAGATGCTAGAGAGGGTCCTTCATTGGCACGAGCATCTCCTCCTCTTGCTACAGCAGTGGTTGTTGATTCGGAATTGAATAGGGTTATGGATATTGTTGAACAAACTCCAATTGTTGAGAAGGTTACTGCTCCAACTACTAGTCAATCTGTTGTTGAGCAAGCCATTGTTCCAATAGTACAGGAGATAGGACAAATTATCTCTAGCCCAGTTGCAAGTTCAGTTCCAACAAACTCTCCAGGTAATGTTAATGTCAATATTTCTACTGGTAATTGCTTTGAGATTCTTTCTCCAATGCAGCAAGGAAAGAACATTGTTGTAGATGAGCAAGAGGAAGTTGGAGAGCGCAATGACAATTTGGTTGGCAATAATAATACAAATTCTACTTTGGTTGAATCTATTGCTCATCAAGCTAAGTTCTGTTGGGGTGATTTGGAAAATGAAGAACCCCTTGGTCATCATACATATGCTCAGGCTGAGATAAGTCCGGTGTGTTCTTGGTTTGATGAGGGTGAAGCAATTCTGGCAGACGAGAATCTTCTTAAGTCTCTCTCAAAGTCTCAAAATAAAAAATTGAAGACCAAGAGAGATTCACGTGATCCTTACCCTACCCTCAATCGAACGCCCGTTGTGCGTCTTGATCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

463

Amino Acids

51.47

Weight (kDa)

8.27

Isoelectric Point (pI)

48.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4283 PF14111 47 - 130 4.4e-07 Domain of unknown function (DUF4283)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 123
AccB1I GGYRCC 2 cut(s) 123, 379
AccI GTMKAC 1 cut(s) 652
AciI CCGC 1 cut(s) 308
AclI AACGTT 1 cut(s) 564
AclWI GGATC 2 cut(s) 446, 1337
AcoI YGGCCR 1 cut(s) 247
AcsI RAATTY 1 cut(s) 1123
AcvI CACGTG 1 cut(s) 1340
AfaI GTAC 2 cut(s) 125, 924
AflII CTTAAG 2 cut(s) 347, 1286
AflIII ACRYGT 1 cut(s) 153
AgsI TTSAA 5 cut(s) 506, 823, 845, 1139, 1321
AhlI ACTAGT 1 cut(s) 884
AjnI CCWGG 2 cut(s) 352, 982
AjuI GAANNNNNNNTTGG 2 cut(s) 1179, 1211
AluBI AGCT 4 cut(s) 294, 580, 599, 1157
AluI AGCT 4 cut(s) 294, 580, 599, 1157
Alw26I GTCTC 4 cut(s) 173, 527, 1296, 1308
AlwI GGATC 2 cut(s) 446, 1337
AlwNI CAGNNNCTG 1 cut(s) 602
AoxI GGCC 1 cut(s) 247
ApeKI GCWGC 3 cut(s) 179, 596, 1042
ApoI RAATTY 1 cut(s) 1123
Asp700I GAANNNNTTC 1 cut(s) 1263
Asp718I GGTACC 1 cut(s) 123
AspLEI GCGC 3 cut(s) 76, 648, 1092
AspS9I GGNCC 1 cut(s) 761
AsuHPI GGTGA 3 cut(s) 251, 1184, 1268
AvaII GGWCC 1 cut(s) 761
BaeGI GKGCMC 2 cut(s) 382, 561
BaeI ACNNNNGTAYC 4 cut(s) 350, 383, 505, 538
BalI TGGCCA 1 cut(s) 249
BanI GGYRCC 2 cut(s) 123, 379
BauI CACGAG 1 cut(s) 774
BbrPI CACGTG 1 cut(s) 1340
BbsI GAAGAC 2 cut(s) 665, 1328
BbvI GCAGC 3 cut(s) 191, 608, 1054
BccI CCATC 4 cut(s) 83, 211, 616, 709
BceAI ACGGC 1 cut(s) 633
BciT130I CCWGG 2 cut(s) 354, 984
BciVI GTATCC 3 cut(s) 272, 351, 506
BcoDI GTCTC 4 cut(s) 173, 527, 1296, 1308
BcuI ACTAGT 1 cut(s) 884
BfaI CTAG 5 cut(s) 21, 318, 753, 885, 948
BfmI CTRYAG 1 cut(s) 795
BfoI RGCGCY 1 cut(s) 649
BfrI CTTAAG 2 cut(s) 347, 1286
BfuI GTATCC 3 cut(s) 272, 351, 506
BisI GCNGC 4 cut(s) 180, 309, 597, 1043
BlsI GCNGC 4 cut(s) 181, 310, 598, 1044
Bme1390I CCNGG 2 cut(s) 354, 984
Bme18I GGWCC 1 cut(s) 761
BmgT120I GGNCC 1 cut(s) 761
BmiI GGNNCC 3 cut(s) 125, 381, 762
BmrFI CCNGG 2 cut(s) 354, 984
BmrI ACTGGG 1 cut(s) 947
BmsI GCATC 4 cut(s) 7, 295, 739, 788
BmuI ACTGGG 1 cut(s) 947
BpiI GAAGAC 2 cut(s) 665, 1328
BpmI CTGGAG 1 cut(s) 966
Bpu10I CCTNAGC 2 cut(s) 419, 1215
BsaAI YACGTR 1 cut(s) 1340
BsaJI CCNNGG 4 cut(s) 183, 267, 353, 1195
BsaWI WCCGGW 1 cut(s) 1231
BsaXI ACNNNNNCTCC 4 cut(s) 480, 510, 964, 994
Bse1I ACTGG 2 cut(s) 953, 1015
Bse3DI GCAATG 2 cut(s) 556, 1099
BseBI CCWGG 2 cut(s) 354, 984
BseDI CCNNGG 4 cut(s) 183, 267, 353, 1195
BseGI GGATG 1 cut(s) 22
BseMI GCAATG 2 cut(s) 556, 1099
BseMII CTCAG 5 cut(s) 353, 433, 681, 1212, 1229
BseNI ACTGG 2 cut(s) 953, 1015
BseRI GAGGAG 2 cut(s) 774, 777
BseSI GKGCMC 2 cut(s) 382, 561
BseXI GCAGC 3 cut(s) 191, 608, 1054
BshFI GGCC 1 cut(s) 249
BshNI GGYRCC 2 cut(s) 123, 379
BsiSI CCGG 1 cut(s) 1232
BsmAI GTCTC 4 cut(s) 173, 527, 1296, 1308
BsmBI CGTCTC 2 cut(s) 173, 527
BsmI GAATGC 2 cut(s) 149, 748
BsnI GGCC 1 cut(s) 249
Bsp1286I GDGCHC 2 cut(s) 382, 561
Bsp143I GATC 4 cut(s) 451, 529, 1342, 1384
Bsp19I CCATGG 1 cut(s) 267
BspACI CCGC 1 cut(s) 308
BspANI GGCC 1 cut(s) 249
BspCNI CTCAG 5 cut(s) 354, 432, 682, 1213, 1228
BspHI TCATGA 1 cut(s) 85
BspLI GGNNCC 3 cut(s) 125, 381, 762
BspPI GGATC 2 cut(s) 446, 1337
BspT107I GGYRCC 2 cut(s) 123, 379
BspTI CTTAAG 2 cut(s) 347, 1286
BsrDI GCAATG 2 cut(s) 556, 1099
BsrI ACTGG 2 cut(s) 953, 1015
BssECI CCNNGG 4 cut(s) 183, 267, 353, 1195
BssMI GATC 4 cut(s) 451, 529, 1342, 1384
BssSI CACGAG 1 cut(s) 774
BssT1I CCWWGG 3 cut(s) 183, 267, 1195
Bst2BI CACGAG 1 cut(s) 774
Bst2UI CCWGG 2 cut(s) 354, 984
Bst4CI ACNGT 2 cut(s) 103, 613
BstAFI CTTAAG 2 cut(s) 347, 1286
BstBAI YACGTR 1 cut(s) 1340
BstC8I GCNNGC 2 cut(s) 539, 906
BstDEI CTNAG 6 cut(s) 362, 419, 690, 1158, 1215, 1221
BstDSI CCRYGG 1 cut(s) 267
BstF5I GGATG 1 cut(s) 22
BstH2I RGCGCY 1 cut(s) 649
BstHHI GCGC 3 cut(s) 76, 648, 1092
BstKTI GATC 4 cut(s) 454, 532, 1345, 1387
BstMAI GTCTC 4 cut(s) 173, 527, 1296, 1308
BstMBI GATC 4 cut(s) 451, 529, 1342, 1384
BstMWI GCNNNNNNNGC 4 cut(s) 73, 1154, 1268, 1375
BstNI CCWGG 2 cut(s) 354, 984
BstNSI RCATGY 2 cut(s) 74, 157
BstSCI CCNGG 2 cut(s) 352, 982
BstSFI CTRYAG 1 cut(s) 795
BstSLI GKGCMC 2 cut(s) 382, 561
BstV1I GCAGC 3 cut(s) 191, 608, 1054
BstV2I GAAGAC 2 cut(s) 665, 1328
BsuI GTATCC 3 cut(s) 272, 351, 506
BsuRI GGCC 1 cut(s) 249
BtgI CCRYGG 1 cut(s) 267
BtsCI GGATG 1 cut(s) 22
BtsI GCAGTG 1 cut(s) 807
BtsIMutI CAGTG 1 cut(s) 807
Cac8I GCNNGC 2 cut(s) 539, 906
CaiI CAGNNNCTG 1 cut(s) 602
CciI TCATGA 1 cut(s) 85
CfoI GCGC 3 cut(s) 76, 648, 1092
Cfr13I GGNCC 1 cut(s) 761
CseI GACGC 2 cut(s) 509, 1367
Csp6I GTAC 2 cut(s) 124, 923
CspCI CAANNNNNGTGG 2 cut(s) 447, 482
CviAII CATG 6 cut(s) 71, 86, 154, 268, 461, 593
CviQI GTAC 2 cut(s) 124, 923
DdeI CTNAG 6 cut(s) 362, 419, 690, 1158, 1215, 1221
DpnI GATC 4 cut(s) 453, 531, 1344, 1386
DpnII GATC 4 cut(s) 451, 529, 1342, 1384
EaeI YGGCCR 1 cut(s) 247
Eco130I CCWWGG 3 cut(s) 183, 267, 1195
Eco47I GGWCC 1 cut(s) 761
Eco72I CACGTG 1 cut(s) 1340
EcoO109I RGGNCCY 1 cut(s) 761
EcoRII CCWGG 2 cut(s) 352, 982
EcoT14I CCWWGG 3 cut(s) 183, 267, 1195
ErhI CCWWGG 3 cut(s) 183, 267, 1195
Esp3I CGTCTC 2 cut(s) 173, 527
FaeI CATG 6 cut(s) 74, 89, 157, 271, 464, 596
FalI AAGNNNNNCTT 4 cut(s) 266, 298, 1179, 1211
FatI CATG 6 cut(s) 70, 85, 153, 267, 460, 592
FauNDI CATATG 1 cut(s) 1210
FblI GTMKAC 1 cut(s) 652
Fnu4HI GCNGC 4 cut(s) 180, 309, 597, 1043
FokI GGATG 1 cut(s) 29
Fsp4HI GCNGC 4 cut(s) 180, 309, 597, 1043
FspBI CTAG 5 cut(s) 21, 318, 753, 885, 948
GlaI GCGC 3 cut(s) 75, 647, 1091
GluI GCNGC 4 cut(s) 180, 309, 597, 1043
GsuI CTGGAG 1 cut(s) 966
HaeII RGCGCY 1 cut(s) 649
HaeIII GGCC 1 cut(s) 249
HapII CCGG 1 cut(s) 1232
HgaI GACGC 2 cut(s) 509, 1367
HhaI GCGC 3 cut(s) 76, 648, 1092
Hin1II CATG 6 cut(s) 74, 89, 157, 271, 464, 596
Hin6I GCGC 3 cut(s) 74, 646, 1090
HinP1I GCGC 3 cut(s) 74, 646, 1090
HincII GTYRAC 1 cut(s) 653
HindII GTYRAC 1 cut(s) 653
HindIII AAGCTT 1 cut(s) 578
HinfI GANTC 5 cut(s) 812, 1027, 1139, 1279, 1334
HpaII CCGG 1 cut(s) 1232
HphI GGTGA 3 cut(s) 251, 1184, 1268
Hpy166II GTNNAC 1 cut(s) 653
Hpy188I TCNGA 7 cut(s) 11, 115, 208, 304, 451, 609, 817
Hpy188III TCNNGA 2 cut(s) 86, 1382
Hpy8I GTNNAC 1 cut(s) 653
Hpy99I CGWCG 3 cut(s) 480, 654, 657
HpyAV CCTTC 4 cut(s) 57, 325, 774, 859
HpyCH4III ACNGT 2 cut(s) 103, 613
HpyCH4IV ACGT 2 cut(s) 564, 1339
HpyCH4V TGCA 8 cut(s) 147, 338, 397, 596, 680, 746, 959, 1042
HpyF10VI GCNNNNNNNGC 4 cut(s) 73, 1154, 1268, 1375
HpyF3I CTNAG 6 cut(s) 362, 419, 690, 1158, 1215, 1221
HpySE526I ACGT 2 cut(s) 564, 1339
Hsp92II CATG 6 cut(s) 74, 89, 157, 271, 464, 596
HspAI GCGC 3 cut(s) 74, 646, 1090
KpnI GGTACC 1 cut(s) 127
Kzo9I GATC 4 cut(s) 451, 529, 1342, 1384
LmnI GCTCC 2 cut(s) 730, 880
Lsp1109I GCAGC 3 cut(s) 191, 608, 1054
LweI GCATC 4 cut(s) 7, 295, 739, 788
MaeI CTAG 5 cut(s) 21, 318, 753, 885, 948
MaeII ACGT 2 cut(s) 564, 1339
MaeIII GTNAC 3 cut(s) 103, 565, 868
MalI GATC 4 cut(s) 453, 531, 1344, 1386
MboI GATC 4 cut(s) 451, 529, 1342, 1384
MboII GAAGA 7 cut(s) 152, 438, 670, 724, 1199, 1274, 1333
MfeI CAATTG 2 cut(s) 339, 855
MhlI GDGCHC 2 cut(s) 382, 561
MlsI TGGCCA 1 cut(s) 249
MluNI TGGCCA 1 cut(s) 249
MmeI TCCRAC 4 cut(s) 490, 902, 995, 1063
Mox20I TGGCCA 1 cut(s) 249
MroXI GAANNNNTTC 1 cut(s) 1263
MscI TGGCCA 1 cut(s) 249
MseI TTAA 3 cut(s) 348, 993, 1287
MslI CAYNNNNRTG 1 cut(s) 1209
Msp20I TGGCCA 1 cut(s) 249
MspCI CTTAAG 2 cut(s) 347, 1286
MspI CCGG 1 cut(s) 1232
MspR9I CCNGG 2 cut(s) 354, 984
MunI CAATTG 2 cut(s) 339, 855
Mva1269I GAATGC 2 cut(s) 149, 748
MvaI CCWGG 2 cut(s) 354, 984
MwoI GCNNNNNNNGC 4 cut(s) 73, 1154, 1268, 1375
NcoI CCATGG 1 cut(s) 267
NdeI CATATG 1 cut(s) 1210
NdeII GATC 4 cut(s) 451, 529, 1342, 1384
NlaIII CATG 6 cut(s) 74, 89, 157, 271, 464, 596
NlaIV GGNNCC 3 cut(s) 125, 381, 762
NmuCI GTSAC 1 cut(s) 103
NspI RCATGY 2 cut(s) 74, 157
PagI TCATGA 1 cut(s) 85
PciI ACATGT 1 cut(s) 153
PctI GAATGC 2 cut(s) 149, 748
PdmI GAANNNNTTC 1 cut(s) 1263
PfeI GAWTC 5 cut(s) 812, 1027, 1139, 1279, 1334
PkrI GCNGC 4 cut(s) 181, 310, 598, 1044
PmaCI CACGTG 1 cut(s) 1340
PmlI CACGTG 1 cut(s) 1340
Ppu21I YACGTR 1 cut(s) 1340
PpuMI RGGWCCY 1 cut(s) 761
PscI ACATGT 1 cut(s) 153
Psp1406I AACGTT 1 cut(s) 564
Psp5II RGGWCCY 1 cut(s) 761
Psp6I CCWGG 2 cut(s) 352, 982
PspCI CACGTG 1 cut(s) 1340
PspGI CCWGG 2 cut(s) 352, 982
PspN4I GGNNCC 3 cut(s) 125, 381, 762
PspPI GGNCC 1 cut(s) 761
PspPPI RGGWCCY 1 cut(s) 761
PstNI CAGNNNCTG 1 cut(s) 602
RsaI GTAC 2 cut(s) 125, 924
RsaNI GTAC 2 cut(s) 124, 923
RseI CAYNNNNRTG 1 cut(s) 1209
SalI GTCGAC 1 cut(s) 651
SaqAI TTAA 3 cut(s) 348, 993, 1287
SatI GCNGC 4 cut(s) 180, 309, 597, 1043
Sau3AI GATC 4 cut(s) 451, 529, 1342, 1384
Sau96I GGNCC 1 cut(s) 761
ScrFI CCNGG 2 cut(s) 354, 984
SduI GDGCHC 2 cut(s) 382, 561
SfaNI GCATC 4 cut(s) 7, 295, 739, 788
SfcI CTRYAG 1 cut(s) 795
SgrDI CGTCGACG 1 cut(s) 651
SinI GGWCC 1 cut(s) 761
SmiMI CAYNNNNRTG 1 cut(s) 1209
SmlI CTYRAG 2 cut(s) 347, 1286
SmoI CTYRAG 2 cut(s) 347, 1286
SpeI ACTAGT 1 cut(s) 884
SsiI CCGC 1 cut(s) 308
SspI AATATT 1 cut(s) 1003
SspMI CTAG 5 cut(s) 21, 318, 753, 885, 948
StyD4I CCNGG 2 cut(s) 352, 982
StyI CCWWGG 3 cut(s) 183, 267, 1195
TaaI ACNGT 2 cut(s) 103, 613
TaiI ACGT 2 cut(s) 567, 1342
TaqI TCGA 2 cut(s) 652, 1363
TatI WGTACW 1 cut(s) 922
TauI GCSGC 1 cut(s) 311
TfiI GAWTC 5 cut(s) 812, 1027, 1139, 1279, 1334
Tru1I TTAA 3 cut(s) 348, 993, 1287
Tru9I TTAA 3 cut(s) 348, 993, 1287
TscAI CASTG 1 cut(s) 807
TseFI GTSAC 1 cut(s) 103
TseI GCWGC 3 cut(s) 179, 596, 1042
Tsp45I GTSAC 1 cut(s) 103
TspDTI ATGAA 4 cut(s) 74, 477, 756, 1200
TspGWI ACGGA 3 cut(s) 147, 501, 670
TspRI CASTG 1 cut(s) 807
Vha464I CTTAAG 2 cut(s) 347, 1286
VpaK11BI GGWCC 1 cut(s) 761
XapI RAATTY 1 cut(s) 1123
XceI RCATGY 2 cut(s) 74, 157
XmiI GTMKAC 1 cut(s) 652
XmnI GAANNNNTTC 1 cut(s) 1263
XspI CTAG 5 cut(s) 21, 318, 753, 885, 948
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.