Rh4BG344900

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0001093
Physical Location & Seq
Forward (+)
50763606 .. 50765628
2023 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG344900.1

Sequence Viewer

Length: 1017 bp
ATGGAAATCCCAGTAGTGAATAGAATAAGCGATTTCGAGCTGCCCAGCCCGTCTTTCCAGTCGCAGGCTTTCTCCATTTCCGGAATCGAAAAGGTCTACAGTGTCTGGACATGGGGCGCTCTAATACTCGCCGTAATAGCTTCCTTCGGCACCATCATCACTAGAATCAACTTTGTTATTATCCGACGGCTCCGCCACAAGACCGCCGAGCCTCTCTGCCTCCGAGAAGAAGACGATTTCTCCGATATCGACGACGACGATGACGAAACATATTCCGTTTCCACGTCATCGTCGGTTTCCGACGAGGAGGACGAGGAAGAAGAGGACGACAGAAGAGACGAAGATTTCAATGTCTCGGGAGCTGACCGGGGACGGACCCGTAATTTGAGGCTCCGCCGTGGCAGAAGCTTCGCGGGCTTGAACTTAAATCGGTTCTCGTGGTCCGACTTCGCCAGCGGCAAGAGCGTCGTGAAGCTCTGGGACAACTTAGCTTTAGGATTGGACCTTGATGACGACAGCGACGACGAATATTCCGGCACCGGCAGAACCGTCTCCGTCTACGACATGAACAAACAGAAGAAGATAAACTCCTTCTTCGCCGGCAGCTTCCAGGTCCCGGCGGCCGCAACGTCTTCGCGGTCCACGATTCTCACGGCTGCTGACACGAACGCCTCCGGGCGCGTAGCCTTGGGCGTGTGGGACACCCGCGTGGGCAGCAGGATCCCGGCGATGCTCGCCGAGTGGGGCCCGCAGATCGGGAGAATCGTCGGAGTGTCCTCGGGCGGCGTCGACAAGGTTTACGTCAAGGATGACGTTACGGGGGCGGTAATGGTCGGTGACGTGAGGAAAGTGGGAGCTCCGTTACGAAACGTAACGGATGCTGACGTGAGCGATACGTGGTGGGACGCCGATGCGGTTATGGTCTCGGACGAGTGTTTTGATGAGTCGGCCGCCGCGAGTGGTTGCGACTCGGCCGTGACTCGGTGTTGTAGCGCGGTTAGGTCTTATCTGTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

338

Amino Acids

36.99

Weight (kDa)

4.46

Isoelectric Point (pI)

38.72

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 149, 536
AccI GTMKAC 3 cut(s) 96, 558, 789
AccII CGCG 6 cut(s) 413, 637, 681, 708, 956, 995
AccIII TCCGGA 1 cut(s) 80
AclWI GGATC 2 cut(s) 715, 728
AcoI YGGCCR 3 cut(s) 621, 948, 972
AcyI GRCGYC 2 cut(s) 786, 906
AfiI CCNNNNNNNGG 4 cut(s) 64, 616, 755, 981
AgsI TTSAA 2 cut(s) 349, 421
AjiI CACGTC 3 cut(s) 285, 841, 886
AjnI CCWGG 1 cut(s) 609
AleI CACNNNNGTG 1 cut(s) 707
AluBI AGCT 8 cut(s) 40, 140, 362, 408, 475, 491, 606, 857
AluI AGCT 8 cut(s) 40, 140, 362, 408, 475, 491, 606, 857
Alw21I GWGCWC 1 cut(s) 859
Alw26I GTCTC 4 cut(s) 330, 358, 556, 928
AlwI GGATC 2 cut(s) 715, 728
AlwNI CAGNNNCTG 1 cut(s) 105
Ama87I CYCGRG 2 cut(s) 355, 778
Aor13HI TCCGGA 1 cut(s) 80
AoxI GGCC 4 cut(s) 621, 745, 948, 972
ApaI GGGCCC 1 cut(s) 749
ApeKI GCWGC 4 cut(s) 40, 603, 656, 714
ArsI GACNNNNNNTTYG 4 cut(s) 367, 399, 920, 952
AspLEI GCGC 3 cut(s) 119, 681, 995
AspS9I GGNCC 7 cut(s) 375, 441, 502, 613, 639, 745, 746
AsuC2I CCSGG 4 cut(s) 368, 617, 676, 725
AsuHPI GGTGA 1 cut(s) 848
AvaI CYCGRG 2 cut(s) 355, 778
AvaII GGWCC 5 cut(s) 375, 441, 502, 613, 639
BaeGI GKGCMC 1 cut(s) 749
BamHI GGATCC 1 cut(s) 720
BanI GGYRCC 2 cut(s) 149, 536
BanII GRGCYC 2 cut(s) 749, 859
BauI CACGAG 1 cut(s) 436
BbsI GAAGAC 2 cut(s) 237, 624
Bbv12I GWGCWC 1 cut(s) 859
BbvI GCAGC 4 cut(s) 27, 615, 643, 726
BccI CCATC 1 cut(s) 161
BceAI ACGGC 5 cut(s) 116, 203, 381, 669, 959
BcgI CGANNNNNNTGC 6 cut(s) 391, 425, 448, 482, 615, 649
BciT130I CCWGG 1 cut(s) 611
BcnI CCSGG 4 cut(s) 368, 617, 676, 725
BcoDI GTCTC 4 cut(s) 330, 358, 556, 928
BfaI CTAG 1 cut(s) 162
BfmI CTRYAG 1 cut(s) 97
BfoI RGCGCY 1 cut(s) 120
Bme1390I CCNGG 5 cut(s) 368, 611, 617, 676, 725
Bme18I GGWCC 5 cut(s) 375, 441, 502, 613, 639
BmeT110I CYCGRG 2 cut(s) 355, 778
BmgBI CACGTC 3 cut(s) 285, 841, 886
BmgT120I GGNCC 7 cut(s) 375, 441, 502, 613, 639, 745, 746
BmiI GGNNCC 9 cut(s) 151, 191, 377, 392, 538, 615, 722, 746, 747
BmrFI CCNGG 5 cut(s) 368, 611, 617, 676, 725
BmrI ACTGGG 1 cut(s) 5
BmsI GCATC 3 cut(s) 720, 868, 901
BmuI ACTGGG 1 cut(s) 5
BpiI GAAGAC 2 cut(s) 237, 624
BpuMI CCSGG 4 cut(s) 368, 617, 676, 725
BsaAI YACGTR 1 cut(s) 897
BsaHI GRCGYC 2 cut(s) 786, 906
BsaI GGTCTC 1 cut(s) 928
BsaJI CCNNGG 4 cut(s) 367, 397, 687, 777
BsaWI WCCGGW 1 cut(s) 80
BsaXI ACNNNNNCTCC 2 cut(s) 224, 254
Bsc4I CCNNNNNNNGG 4 cut(s) 64, 616, 755, 981
Bse118I RCCGGY 2 cut(s) 539, 599
Bse1I ACTGG 2 cut(s) 11, 58
BseAI TCCGGA 1 cut(s) 80
BseBI CCWGG 1 cut(s) 611
BseDI CCNNGG 4 cut(s) 367, 397, 687, 777
BseGI GGATG 2 cut(s) 814, 883
BseLI CCNNNNNNNGG 4 cut(s) 64, 616, 755, 981
BseNI ACTGG 2 cut(s) 11, 58
BseRI GAGGAG 1 cut(s) 320
BseSI GKGCMC 1 cut(s) 749
BseX3I CGGCCG 3 cut(s) 621, 948, 972
BseXI GCAGC 4 cut(s) 27, 615, 643, 726
BseYI CCCAGC 1 cut(s) 44
Bsh1236I CGCG 6 cut(s) 413, 637, 681, 708, 956, 995
Bsh1285I CGRYCG 3 cut(s) 624, 951, 975
BshFI GGCC 4 cut(s) 623, 747, 950, 974
BshNI GGYRCC 2 cut(s) 149, 536
BsiEI CGRYCG 3 cut(s) 624, 951, 975
BsiHKAI GWGCWC 1 cut(s) 859
BsiHKCI CYCGRG 2 cut(s) 355, 778
BsiSI CCGG 8 cut(s) 81, 367, 534, 540, 600, 617, 675, 725
BslFI GGGAC 5 cut(s) 384, 494, 599, 713, 917
BslI CCNNNNNNNGG 4 cut(s) 64, 616, 755, 981
BsmAI GTCTC 4 cut(s) 330, 358, 556, 928
BsmBI CGTCTC 2 cut(s) 330, 556
BsmFI GGGAC 5 cut(s) 384, 494, 599, 713, 917
BsnI GGCC 4 cut(s) 623, 747, 950, 974
Bso31I GGTCTC 1 cut(s) 928
BsoBI CYCGRG 2 cut(s) 355, 778
Bsp120I GGGCCC 1 cut(s) 745
Bsp1286I GDGCHC 2 cut(s) 749, 859
Bsp13I TCCGGA 1 cut(s) 80
Bsp143I GATC 2 cut(s) 720, 753
BspANI GGCC 4 cut(s) 623, 747, 950, 974
BspEI TCCGGA 1 cut(s) 80
BspFNI CGCG 6 cut(s) 413, 637, 681, 708, 956, 995
BspLI GGNNCC 9 cut(s) 151, 191, 377, 392, 538, 615, 722, 746, 747
BspPI GGATC 2 cut(s) 715, 728
BspT107I GGYRCC 2 cut(s) 149, 536
BspTNI GGTCTC 1 cut(s) 928
BsrFI RCCGGY 2 cut(s) 539, 599
BsrI ACTGG 2 cut(s) 11, 58
BssAI RCCGGY 2 cut(s) 539, 599
BssECI CCNNGG 4 cut(s) 367, 397, 687, 777
BssMI GATC 2 cut(s) 720, 753
BssNI GRCGYC 2 cut(s) 786, 906
BssSI CACGAG 1 cut(s) 436
BssT1I CCWWGG 1 cut(s) 687
Bst2BI CACGAG 1 cut(s) 436
Bst2UI CCWGG 1 cut(s) 611
Bst4CI ACNGT 2 cut(s) 101, 550
Bst6I CTCTTC 2 cut(s) 315, 328
BstACI GRCGYC 2 cut(s) 786, 906
BstBAI YACGTR 1 cut(s) 897
BstC8I GCNNGC 6 cut(s) 66, 415, 454, 601, 735, 749
BstDEI CTNAG 1 cut(s) 487
BstDSI CCRYGG 1 cut(s) 397
BstF5I GGATG 2 cut(s) 814, 883
BstFNI CGCG 6 cut(s) 413, 637, 681, 708, 956, 995
BstH2I RGCGCY 1 cut(s) 120
BstHHI GCGC 3 cut(s) 119, 681, 995
BstKTI GATC 2 cut(s) 723, 756
BstMAI GTCTC 4 cut(s) 330, 358, 556, 928
BstMBI GATC 2 cut(s) 720, 753
BstMCI CGRYCG 3 cut(s) 624, 951, 975
BstMWI GCNNNNNNNGC 5 cut(s) 137, 414, 462, 714, 734
BstNI CCWGG 1 cut(s) 611
BstSCI CCNGG 5 cut(s) 366, 609, 615, 674, 723
BstSFI CTRYAG 1 cut(s) 97
BstSLI GKGCMC 1 cut(s) 749
BstUI CGCG 6 cut(s) 413, 637, 681, 708, 956, 995
BstV1I GCAGC 4 cut(s) 27, 615, 643, 726
BstV2I GAAGAC 2 cut(s) 237, 624
BstX2I RGATCY 1 cut(s) 720
BstYI RGATCY 1 cut(s) 720
BstZI CGGCCG 3 cut(s) 621, 948, 972
BsuRI GGCC 4 cut(s) 623, 747, 950, 974
BtgI CCRYGG 1 cut(s) 397
BtgZI GCGATG 1 cut(s) 743
BtrI CACGTC 3 cut(s) 285, 841, 886
BtsCI GGATG 2 cut(s) 814, 883
BtsIMutI CAGTG 1 cut(s) 106
Cac8I GCNNGC 6 cut(s) 66, 415, 454, 601, 735, 749
CaiI CAGNNNCTG 1 cut(s) 105
CciNI GCGGCCGC 1 cut(s) 621
CfoI GCGC 3 cut(s) 119, 681, 995
Cfr10I RCCGGY 2 cut(s) 539, 599
Cfr13I GGNCC 7 cut(s) 375, 441, 502, 613, 639, 745, 746
CseI GACGC 3 cut(s) 454, 775, 914
CviAII CATG 2 cut(s) 111, 565
DdeI CTNAG 1 cut(s) 487
DpnI GATC 2 cut(s) 722, 755
DpnII GATC 2 cut(s) 720, 753
EaeI YGGCCR 3 cut(s) 621, 948, 972
EagI CGGCCG 3 cut(s) 621, 948, 972
Eam1104I CTCTTC 2 cut(s) 315, 328
EarI CTCTTC 2 cut(s) 315, 328
EciI GGCGGA 2 cut(s) 182, 383
Ecl136II GAGCTC 1 cut(s) 857
EclXI CGGCCG 3 cut(s) 621, 948, 972
Eco130I CCWWGG 1 cut(s) 687
Eco24I GRGCYC 2 cut(s) 749, 859
Eco31I GGTCTC 1 cut(s) 928
Eco32I GATATC 1 cut(s) 247
Eco47I GGWCC 5 cut(s) 375, 441, 502, 613, 639
Eco52I CGGCCG 3 cut(s) 621, 948, 972
Eco53kI GAGCTC 1 cut(s) 857
Eco88I CYCGRG 2 cut(s) 355, 778
EcoICRI GAGCTC 1 cut(s) 857
EcoO109I RGGNCCY 2 cut(s) 613, 745
EcoRII CCWGG 1 cut(s) 609
EcoRV GATATC 1 cut(s) 247
EcoT14I CCWWGG 1 cut(s) 687
EcoT38I GRGCYC 2 cut(s) 749, 859
ErhI CCWWGG 1 cut(s) 687
Esp3I CGTCTC 2 cut(s) 330, 556
FaeI CATG 2 cut(s) 114, 568
FaiI YATR 5 cut(s) 112, 271, 566, 920, 1015
FaqI GGGAC 5 cut(s) 384, 494, 599, 713, 917
FatI CATG 2 cut(s) 110, 564
FauI CCCGC 3 cut(s) 406, 713, 756
FblI GTMKAC 3 cut(s) 96, 558, 789
FokI GGATG 2 cut(s) 821, 890
FriOI GRGCYC 2 cut(s) 749, 859
FspBI CTAG 1 cut(s) 162
GlaI GCGC 3 cut(s) 118, 680, 994
GsaI CCCAGC 1 cut(s) 48
HaeII RGCGCY 1 cut(s) 120
HaeIII GGCC 4 cut(s) 623, 747, 950, 974
HapII CCGG 8 cut(s) 81, 367, 534, 540, 600, 617, 675, 725
HgaI GACGC 3 cut(s) 454, 775, 914
HhaI GCGC 3 cut(s) 119, 681, 995
Hin1I GRCGYC 2 cut(s) 786, 906
Hin1II CATG 2 cut(s) 114, 568
Hin6I GCGC 3 cut(s) 117, 679, 993
HinP1I GCGC 3 cut(s) 117, 679, 993
HincII GTYRAC 1 cut(s) 790
HindII GTYRAC 1 cut(s) 790
HindIII AAGCTT 1 cut(s) 406
HinfI GANTC 7 cut(s) 84, 165, 646, 762, 944, 968, 979
HpaII CCGG 8 cut(s) 81, 367, 534, 540, 600, 617, 675, 725
HphI GGTGA 1 cut(s) 848
Hpy166II GTNNAC 5 cut(s) 97, 559, 642, 790, 799
Hpy188I TCNGA 7 cut(s) 185, 224, 244, 301, 445, 770, 928
Hpy188III TCNNGA 5 cut(s) 81, 106, 357, 469, 757
Hpy8I GTNNAC 5 cut(s) 97, 559, 642, 790, 799
HpyAV CCTTC 2 cut(s) 154, 601
HpyCH4III ACNGT 2 cut(s) 101, 550
HpyCH4IV ACGT 8 cut(s) 284, 629, 801, 813, 840, 870, 885, 896
HpyF10VI GCNNNNNNNGC 5 cut(s) 137, 414, 462, 714, 734
HpyF3I CTNAG 1 cut(s) 487
HpySE526I ACGT 8 cut(s) 284, 629, 801, 813, 840, 870, 885, 896
Hsp92I GRCGYC 2 cut(s) 786, 906
Hsp92II CATG 2 cut(s) 114, 568
HspAI GCGC 3 cut(s) 117, 679, 993
Kpn2I TCCGGA 1 cut(s) 80
KroI GCCGGC 1 cut(s) 599
KroNI GCCGGC 1 cut(s) 601
Kzo9I GATC 2 cut(s) 720, 753
LmnI GCTCC 5 cut(s) 195, 359, 396, 854, 862
Lsp1109I GCAGC 4 cut(s) 27, 615, 643, 726
LweI GCATC 3 cut(s) 720, 868, 901
MaeI CTAG 1 cut(s) 162
MaeII ACGT 8 cut(s) 284, 629, 801, 813, 840, 870, 885, 896
MaeIII GTNAC 5 cut(s) 814, 836, 861, 871, 976
MalI GATC 2 cut(s) 722, 755
MboI GATC 2 cut(s) 720, 753
MflI RGATCY 1 cut(s) 720
MhlI GDGCHC 2 cut(s) 749, 859
MluCI AATT 1 cut(s) 382
MlyI GAGTC 3 cut(s) 953, 962, 973
MmeI TCCRAC 4 cut(s) 208, 324, 468, 748
MroI TCCGGA 1 cut(s) 80
MroNI GCCGGC 1 cut(s) 599
MseI TTAA 1 cut(s) 425
MslI CAYNNNNRTG 1 cut(s) 707
MspA1I CMGCKG 1 cut(s) 456
MspI CCGG 8 cut(s) 81, 367, 534, 540, 600, 617, 675, 725
MspR9I CCNGG 5 cut(s) 368, 611, 617, 676, 725
MvaI CCWGG 1 cut(s) 611
MvnI CGCG 6 cut(s) 413, 637, 681, 708, 956, 995
MwoI GCNNNNNNNGC 5 cut(s) 137, 414, 462, 714, 734
NaeI GCCGGC 1 cut(s) 601
NciI CCSGG 4 cut(s) 368, 617, 676, 725
NdeII GATC 2 cut(s) 720, 753
NgoMIV GCCGGC 1 cut(s) 599
NlaIII CATG 2 cut(s) 114, 568
NlaIV GGNNCC 9 cut(s) 151, 191, 377, 392, 538, 615, 722, 746, 747
NmeAIII GCCGAG 3 cut(s) 232, 763, 950
NmuCI GTSAC 2 cut(s) 836, 976
NotI GCGGCCGC 1 cut(s) 621
OliI CACNNNNGTG 1 cut(s) 707
PcsI WCGNNNNNNNCGW 8 cut(s) 240, 255, 261, 309, 519, 641, 762, 953
PdiI GCCGGC 1 cut(s) 601
PfeI GAWTC 4 cut(s) 84, 165, 646, 762
PleI GAGTC 3 cut(s) 952, 962, 973
PpsI GAGTC 3 cut(s) 952, 962, 973
Ppu21I YACGTR 1 cut(s) 897
PpuMI RGGWCCY 1 cut(s) 613
Psp124BI GAGCTC 1 cut(s) 859
Psp5II RGGWCCY 1 cut(s) 613
Psp6I CCWGG 1 cut(s) 609
PspFI CCCAGC 1 cut(s) 44
PspGI CCWGG 1 cut(s) 609
PspN4I GGNNCC 9 cut(s) 151, 191, 377, 392, 538, 615, 722, 746, 747
PspOMI GGGCCC 1 cut(s) 745
PspPI GGNCC 7 cut(s) 375, 441, 502, 613, 639, 745, 746
PspPPI RGGWCCY 1 cut(s) 613
PstNI CAGNNNCTG 1 cut(s) 105
PsuI RGATCY 1 cut(s) 720
RseI CAYNNNNRTG 1 cut(s) 707
SacI GAGCTC 1 cut(s) 859
SalI GTCGAC 1 cut(s) 788
SaqAI TTAA 1 cut(s) 425
Sau3AI GATC 2 cut(s) 720, 753
Sau96I GGNCC 7 cut(s) 375, 441, 502, 613, 639, 745, 746
SchI GAGTC 3 cut(s) 953, 962, 973
ScrFI CCNGG 5 cut(s) 368, 611, 617, 676, 725
SduI GDGCHC 2 cut(s) 749, 859
SfaNI GCATC 3 cut(s) 720, 868, 901
SfcI CTRYAG 1 cut(s) 97
SinI GGWCC 5 cut(s) 375, 441, 502, 613, 639
SmiMI CAYNNNNRTG 1 cut(s) 707
Sse9I AATT 1 cut(s) 382
SspI AATATT 1 cut(s) 530
SspMI CTAG 1 cut(s) 162
SstI GAGCTC 1 cut(s) 859
StyD4I CCNGG 5 cut(s) 366, 609, 615, 674, 723
StyI CCWWGG 1 cut(s) 687
TaaI ACNGT 2 cut(s) 101, 550
TaiI ACGT 8 cut(s) 287, 632, 804, 816, 843, 873, 888, 899
TaqI TCGA 4 cut(s) 36, 87, 249, 789
TasI AATT 1 cut(s) 382
TauI GCSGC 6 cut(s) 459, 623, 626, 786, 953, 956
TfiI GAWTC 4 cut(s) 84, 165, 646, 762
Tru1I TTAA 1 cut(s) 425
Tru9I TTAA 1 cut(s) 425
TscAI CASTG 1 cut(s) 106
TseFI GTSAC 2 cut(s) 836, 976
TseI GCWGC 4 cut(s) 40, 603, 656, 714
Tsp45I GTSAC 2 cut(s) 836, 976
TspDTI ATGAA 1 cut(s) 581
TspGWI ACGGA 5 cut(s) 265, 388, 544, 849, 890
TspRI CASTG 1 cut(s) 106
VpaK11BI GGWCC 5 cut(s) 375, 441, 502, 613, 639
XmiI GTMKAC 3 cut(s) 96, 558, 789
XspI CTAG 1 cut(s) 162
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.