FvH4_4g27210

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0001093
Physical Location & Seq
Forward (+)
28303105 .. 28304869
1765 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g27210.t1

Sequence Viewer

Length: 1014 bp
ATGGAAATCCCAGTAGTGAATAGAATAAGCCATTTCGAGGTAACCGCCTTGCCGATCCCGTCTTTCCCTTCTCAGAGTACTTTCTCCATTTCCGGAATCGAAAAGGTCTTTAGTGTCTGGACATGGGGTGCTTTAATACTCGCCGTAATAGCTTCCTTTGGCACCATCATCACTAGAGTCAACTTTGTTATCATCAGGAGGCTTCGCCACAACACCTCAGAGCCTATCTCCGTCTTAGAAGAAGATGATTTCTCCGATATCGACGACGACGATGACGACGCGTATTCCGTTTCTACGTCCTCGTCGGTTTTCGATGAGGAGGAGGAAGACGATGAGGACAGAAGAGATGAAGATTTTAAAGTCTTGGGATCCGGCCGTAATTTGAGGCTCCGCCGTGGCAGAAGTTTCGCCGGCTTGAACTTAAACCGGTTCTCCTGGTCCGACTTCGCCAGCGGCAAGAGCGTCGTGAACCTCTGGGACAACTTAGCTTTAGGATTGGACCATGATGACGACAGCGACGTCGACGAATATTCCGGCAGCGGCAGAGTCGTCTCCGTCTACGACATGAACAAGCAGAAGAAGATAAACTCCTTCTTCGCCGGAAGCTTCCAGGTCCCAGCGGCCGCCACGTCATCACGGTCCACGATTCTCACGGCGGACACGAACGCCTCCGGGCGCGTGGCGTTGGGCGTGTGGGACACGCGCGTGGGCAGCAGGATTCCGGCGATGATGGCCGAGTGGGGGCCTCAGATCGGGAGAATCGTCGGGGTGTCGTCTGGCGGCGTCGATAAAGTTTACGTCAAGGACGACGTTAGGGGCGCGTTAATGGTTGGTGACGTGAGGAAGGTGGGAGCTCCGTTACGAAACGTAACGGATGCTGACGTGAGCGATACGTGGTGGGACGCCGACGCGGTTATGGTCTCCGACGAGTGTTTTGATGAGGTTGCCGCCGCGAATGGTTGCGACTCGGCCGTGACTCGGTGTTGTAGTGCCGTTAGGTCTTATCTGTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

338

Amino Acids

36.86

Weight (kDa)

4.48

Isoelectric Point (pI)

37.0

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 518
AatII GACGTC 1 cut(s) 522
AccB1I GGYRCC 1 cut(s) 161
AccI GTMKAC 2 cut(s) 522, 558
AccII CGCG 7 cut(s) 281, 678, 703, 705, 821, 911, 953
AccIII TCCGGA 1 cut(s) 92
AclWI GGATC 3 cut(s) 49, 363, 376
AcoI YGGCCR 4 cut(s) 373, 621, 732, 969
AcyI GRCGYC 3 cut(s) 519, 783, 903
AfaI GTAC 1 cut(s) 79
AfiI CCNNNNNNNGG 4 cut(s) 37, 741, 752, 978
AflIII ACRYGT 1 cut(s) 279
AgeI ACCGGT 1 cut(s) 426
AgsI TTSAA 1 cut(s) 418
AjiI CACGTC 3 cut(s) 630, 838, 883
AjnI CCWGG 2 cut(s) 434, 609
AleI CACNNNNGTG 1 cut(s) 704
AluBI AGCT 4 cut(s) 152, 488, 606, 854
AluI AGCT 4 cut(s) 152, 488, 606, 854
Alw21I GWGCWC 1 cut(s) 856
Alw26I GTCTC 2 cut(s) 556, 925
AlwI GGATC 3 cut(s) 49, 363, 376
Aor13HI TCCGGA 1 cut(s) 92
AoxI GGCC 5 cut(s) 373, 621, 732, 743, 969
ApeKI GCWGC 2 cut(s) 537, 711
ArsI GACNNNNNNTTYG 2 cut(s) 917, 949
AsiGI ACCGGT 1 cut(s) 426
AspLEI GCGC 3 cut(s) 678, 705, 821
AspS9I GGNCC 5 cut(s) 438, 499, 613, 639, 743
AsuC2I CCSGG 1 cut(s) 673
AsuHPI GGTGA 1 cut(s) 845
AvaII GGWCC 4 cut(s) 438, 499, 613, 639
BamHI GGATCC 1 cut(s) 368
BanI GGYRCC 1 cut(s) 161
BanII GRGCYC 1 cut(s) 856
BbsI GAAGAC 1 cut(s) 333
Bbv12I GWGCWC 1 cut(s) 856
BbvI GCAGC 2 cut(s) 549, 723
BccI CCATC 2 cut(s) 173, 724
BceAI ACGGC 6 cut(s) 128, 360, 378, 669, 956, 977
BcgI CGANNNNNNTGC 4 cut(s) 388, 422, 445, 479
BciT130I CCWGG 2 cut(s) 436, 611
BcnI CCSGG 1 cut(s) 673
BcoDI GTCTC 2 cut(s) 556, 925
BfaI CTAG 1 cut(s) 174
BisI GCNGC 9 cut(s) 454, 538, 541, 621, 624, 712, 781, 948, 951
BlsI GCNGC 9 cut(s) 455, 539, 542, 622, 625, 713, 782, 949, 952
BmcAI AGTACT 1 cut(s) 79
Bme1390I CCNGG 3 cut(s) 436, 611, 673
Bme18I GGWCC 4 cut(s) 438, 499, 613, 639
BmgBI CACGTC 3 cut(s) 630, 838, 883
BmgT120I GGNCC 5 cut(s) 438, 499, 613, 639, 743
BmiI GGNNCC 5 cut(s) 163, 370, 389, 615, 744
BmrFI CCNGG 3 cut(s) 436, 611, 673
BmrI ACTGGG 1 cut(s) 5
BmsI GCATC 1 cut(s) 865
BmuI ACTGGG 1 cut(s) 5
BpiI GAAGAC 1 cut(s) 333
BplI GAGNNNNNCTC 2 cut(s) 212, 244
BpuMI CCSGG 1 cut(s) 673
BsaAI YACGTR 1 cut(s) 894
BsaHI GRCGYC 3 cut(s) 519, 783, 903
BsaI GGTCTC 1 cut(s) 925
BsaJI CCNNGG 1 cut(s) 394
BsaWI WCCGGW 2 cut(s) 92, 426
BsaXI ACNNNNNCTCC 2 cut(s) 416, 446
Bsc4I CCNNNNNNNGG 4 cut(s) 37, 741, 752, 978
Bse118I RCCGGY 2 cut(s) 410, 426
Bse1I ACTGG 1 cut(s) 11
BseAI TCCGGA 1 cut(s) 92
BseBI CCWGG 2 cut(s) 436, 611
BseDI CCNNGG 1 cut(s) 394
BseGI GGATG 1 cut(s) 880
BseLI CCNNNNNNNGG 4 cut(s) 37, 741, 752, 978
BseMII CTCAG 3 cut(s) 86, 231, 761
BseNI ACTGG 1 cut(s) 11
BseRI GAGGAG 2 cut(s) 332, 335
BseX3I CGGCCG 3 cut(s) 373, 621, 969
BseXI GCAGC 2 cut(s) 549, 723
BseYI CCCAGC 1 cut(s) 616
Bsh1236I CGCG 7 cut(s) 281, 678, 703, 705, 821, 911, 953
Bsh1285I CGRYCG 3 cut(s) 376, 624, 972
BshFI GGCC 5 cut(s) 375, 623, 734, 745, 971
BshNI GGYRCC 1 cut(s) 161
BshTI ACCGGT 1 cut(s) 426
BsiEI CGRYCG 3 cut(s) 376, 624, 972
BsiHKAI GWGCWC 1 cut(s) 856
BsiSI CCGG 8 cut(s) 93, 372, 411, 427, 534, 600, 672, 722
BslFI GGGAC 4 cut(s) 491, 599, 710, 914
BslI CCNNNNNNNGG 4 cut(s) 37, 741, 752, 978
BsmAI GTCTC 2 cut(s) 556, 925
BsmBI CGTCTC 1 cut(s) 556
BsmFI GGGAC 4 cut(s) 491, 599, 710, 914
BsnI GGCC 5 cut(s) 375, 623, 734, 745, 971
Bso31I GGTCTC 1 cut(s) 925
Bsp1286I GDGCHC 1 cut(s) 856
Bsp13I TCCGGA 1 cut(s) 92
Bsp143I GATC 3 cut(s) 54, 368, 750
BspANI GGCC 5 cut(s) 375, 623, 734, 745, 971
BspCNI CTCAG 3 cut(s) 85, 230, 760
BspEI TCCGGA 1 cut(s) 92
BspFNI CGCG 7 cut(s) 281, 678, 703, 705, 821, 911, 953
BspLI GGNNCC 5 cut(s) 163, 370, 389, 615, 744
BspPI GGATC 3 cut(s) 49, 363, 376
BspT107I GGYRCC 1 cut(s) 161
BspTNI GGTCTC 1 cut(s) 925
BsrFI RCCGGY 2 cut(s) 410, 426
BsrI ACTGG 1 cut(s) 11
BssAI RCCGGY 2 cut(s) 410, 426
BssECI CCNNGG 1 cut(s) 394
BssMI GATC 3 cut(s) 54, 368, 750
BssNI GRCGYC 3 cut(s) 519, 783, 903
Bst2UI CCWGG 2 cut(s) 436, 611
Bst4CI ACNGT 1 cut(s) 639
Bst6I CTCTTC 1 cut(s) 337
BstACI GRCGYC 3 cut(s) 519, 783, 903
BstBAI YACGTR 1 cut(s) 894
BstC8I GCNNGC 2 cut(s) 412, 451
BstDEI CTNAG 5 cut(s) 72, 217, 235, 484, 747
BstDSI CCRYGG 1 cut(s) 394
BstEII GGTNACC 1 cut(s) 40
BstF5I GGATG 1 cut(s) 880
BstFNI CGCG 7 cut(s) 281, 678, 703, 705, 821, 911, 953
BstHHI GCGC 3 cut(s) 678, 705, 821
BstKTI GATC 3 cut(s) 57, 371, 753
BstMAI GTCTC 2 cut(s) 556, 925
BstMBI GATC 3 cut(s) 54, 368, 750
BstMCI CGRYCG 3 cut(s) 376, 624, 972
BstMWI GCNNNNNNNGC 4 cut(s) 149, 459, 711, 731
BstNI CCWGG 2 cut(s) 436, 611
BstPI GGTNACC 1 cut(s) 40
BstSCI CCNGG 3 cut(s) 434, 609, 671
BstUI CGCG 7 cut(s) 281, 678, 703, 705, 821, 911, 953
BstV1I GCAGC 2 cut(s) 549, 723
BstV2I GAAGAC 1 cut(s) 333
BstX2I RGATCY 1 cut(s) 368
BstYI RGATCY 1 cut(s) 368
BstZI CGGCCG 3 cut(s) 373, 621, 969
BsuRI GGCC 5 cut(s) 375, 623, 734, 745, 971
BtgI CCRYGG 1 cut(s) 394
BtgZI GCGATG 1 cut(s) 740
BtrI CACGTC 3 cut(s) 630, 838, 883
BtsCI GGATG 1 cut(s) 880
Cac8I GCNNGC 2 cut(s) 412, 451
CciNI GCGGCCGC 1 cut(s) 621
CfoI GCGC 3 cut(s) 678, 705, 821
Cfr10I RCCGGY 2 cut(s) 410, 426
Cfr13I GGNCC 5 cut(s) 438, 499, 613, 639, 743
CseI GACGC 5 cut(s) 287, 451, 772, 911, 917
Csp6I GTAC 1 cut(s) 78
CspAI ACCGGT 1 cut(s) 426
CviAII CATG 3 cut(s) 123, 503, 565
CviQI GTAC 1 cut(s) 78
DdeI CTNAG 5 cut(s) 72, 217, 235, 484, 747
DpnI GATC 3 cut(s) 56, 370, 752
DpnII GATC 3 cut(s) 54, 368, 750
DraI TTTAAA 1 cut(s) 358
DrdI GACNNNNNNGTC 1 cut(s) 518
DseDI GACNNNNNNGTC 1 cut(s) 518
EaeI YGGCCR 4 cut(s) 373, 621, 732, 969
EagI CGGCCG 3 cut(s) 373, 621, 969
Eam1104I CTCTTC 1 cut(s) 337
EarI CTCTTC 1 cut(s) 337
EciI GGCGGA 2 cut(s) 380, 671
Ecl136II GAGCTC 1 cut(s) 854
EclXI CGGCCG 3 cut(s) 373, 621, 969
Eco24I GRGCYC 1 cut(s) 856
Eco31I GGTCTC 1 cut(s) 925
Eco32I GATATC 1 cut(s) 259
Eco47I GGWCC 4 cut(s) 438, 499, 613, 639
Eco52I CGGCCG 3 cut(s) 373, 621, 969
Eco53kI GAGCTC 1 cut(s) 854
Eco91I GGTNACC 1 cut(s) 40
EcoICRI GAGCTC 1 cut(s) 854
EcoO109I RGGNCCY 2 cut(s) 613, 743
EcoO65I GGTNACC 1 cut(s) 40
EcoRII CCWGG 2 cut(s) 434, 609
EcoRV GATATC 1 cut(s) 259
EcoT38I GRGCYC 1 cut(s) 856
Esp3I CGTCTC 1 cut(s) 556
FaeI CATG 3 cut(s) 126, 506, 568
FaiI YATR 5 cut(s) 124, 504, 566, 917, 1012
FaqI GGGAC 4 cut(s) 491, 599, 710, 914
FatI CATG 3 cut(s) 122, 502, 564
FblI GTMKAC 2 cut(s) 522, 558
Fnu4HI GCNGC 9 cut(s) 454, 538, 541, 621, 624, 712, 781, 948, 951
FokI GGATG 1 cut(s) 887
FriOI GRGCYC 1 cut(s) 856
Fsp4HI GCNGC 9 cut(s) 454, 538, 541, 621, 624, 712, 781, 948, 951
FspBI CTAG 1 cut(s) 174
GlaI GCGC 3 cut(s) 677, 704, 820
GluI GCNGC 9 cut(s) 454, 538, 541, 621, 624, 712, 781, 948, 951
GsaI CCCAGC 1 cut(s) 620
HaeIII GGCC 5 cut(s) 375, 623, 734, 745, 971
HapII CCGG 8 cut(s) 93, 372, 411, 427, 534, 600, 672, 722
HgaI GACGC 5 cut(s) 287, 451, 772, 911, 917
HhaI GCGC 3 cut(s) 678, 705, 821
Hin1I GRCGYC 3 cut(s) 519, 783, 903
Hin1II CATG 3 cut(s) 126, 506, 568
Hin6I GCGC 3 cut(s) 676, 703, 819
HinP1I GCGC 3 cut(s) 676, 703, 819
HincII GTYRAC 2 cut(s) 181, 523
HindII GTYRAC 2 cut(s) 181, 523
HindIII AAGCTT 1 cut(s) 604
HinfI GANTC 8 cut(s) 96, 177, 546, 646, 718, 759, 965, 976
HpaII CCGG 8 cut(s) 93, 372, 411, 427, 534, 600, 672, 722
HphI GGTGA 1 cut(s) 845
Hpy166II GTNNAC 6 cut(s) 181, 469, 523, 559, 642, 796
Hpy188I TCNGA 6 cut(s) 75, 220, 256, 442, 750, 925
Hpy188III TCNNGA 5 cut(s) 93, 118, 196, 466, 754
Hpy8I GTNNAC 6 cut(s) 181, 469, 523, 559, 642, 796
HpyAV CCTTC 3 cut(s) 78, 601, 838
HpyCH4III ACNGT 1 cut(s) 639
HpyCH4IV ACGT 9 cut(s) 296, 519, 629, 798, 810, 837, 867, 882, 893
HpyF10VI GCNNNNNNNGC 4 cut(s) 149, 459, 711, 731
HpyF3I CTNAG 5 cut(s) 72, 217, 235, 484, 747
HpySE526I ACGT 9 cut(s) 296, 519, 629, 798, 810, 837, 867, 882, 893
Hsp92I GRCGYC 3 cut(s) 519, 783, 903
Hsp92II CATG 3 cut(s) 126, 506, 568
HspAI GCGC 3 cut(s) 676, 703, 819
Kpn2I TCCGGA 1 cut(s) 92
KroI GCCGGC 1 cut(s) 410
KroNI GCCGGC 1 cut(s) 412
Kzo9I GATC 3 cut(s) 54, 368, 750
LmnI GCTCC 3 cut(s) 393, 851, 859
Lsp1109I GCAGC 2 cut(s) 549, 723
LweI GCATC 1 cut(s) 865
MaeI CTAG 1 cut(s) 174
MaeII ACGT 9 cut(s) 296, 519, 629, 798, 810, 837, 867, 882, 893
MaeIII GTNAC 5 cut(s) 40, 833, 858, 868, 973
MalI GATC 3 cut(s) 56, 370, 752
MboI GATC 3 cut(s) 54, 368, 750
MboII GAAGA 8 cut(s) 251, 254, 338, 354, 362, 586, 589, 592
MflI RGATCY 1 cut(s) 368
MhlI GDGCHC 1 cut(s) 856
MluCI AATT 1 cut(s) 379
MluI ACGCGT 1 cut(s) 279
MlyI GAGTC 4 cut(s) 186, 555, 959, 970
MmeI TCCRAC 2 cut(s) 465, 948
MroI TCCGGA 1 cut(s) 92
MroNI GCCGGC 1 cut(s) 410
MseI TTAA 4 cut(s) 134, 357, 422, 824
MslI CAYNNNNRTG 1 cut(s) 704
MspA1I CMGCKG 3 cut(s) 453, 540, 620
MspI CCGG 8 cut(s) 93, 372, 411, 427, 534, 600, 672, 722
MspR9I CCNGG 3 cut(s) 436, 611, 673
MvaI CCWGG 2 cut(s) 436, 611
MvnI CGCG 7 cut(s) 281, 678, 703, 705, 821, 911, 953
MwoI GCNNNNNNNGC 4 cut(s) 149, 459, 711, 731
NaeI GCCGGC 1 cut(s) 412
NciI CCSGG 1 cut(s) 673
NdeII GATC 3 cut(s) 54, 368, 750
NgoMIV GCCGGC 1 cut(s) 410
NlaIII CATG 3 cut(s) 126, 506, 568
NlaIV GGNNCC 5 cut(s) 163, 370, 389, 615, 744
NmeAIII GCCGAG 2 cut(s) 760, 947
NmuCI GTSAC 2 cut(s) 833, 973
NotI GCGGCCGC 1 cut(s) 621
OliI CACNNNNGTG 1 cut(s) 704
PcsI WCGNNNNNNNCGW 7 cut(s) 267, 273, 285, 516, 659, 759, 804
PdiI GCCGGC 1 cut(s) 412
PfeI GAWTC 4 cut(s) 96, 646, 718, 759
PinAI ACCGGT 1 cut(s) 426
PkrI GCNGC 9 cut(s) 455, 539, 542, 622, 625, 713, 782, 949, 952
PleI GAGTC 4 cut(s) 185, 554, 959, 970
PpsI GAGTC 4 cut(s) 185, 554, 959, 970
Ppu21I YACGTR 1 cut(s) 894
PpuMI RGGWCCY 1 cut(s) 613
Psp124BI GAGCTC 1 cut(s) 856
Psp5II RGGWCCY 1 cut(s) 613
Psp6I CCWGG 2 cut(s) 434, 609
PspEI GGTNACC 1 cut(s) 40
PspFI CCCAGC 1 cut(s) 616
PspGI CCWGG 2 cut(s) 434, 609
PspN4I GGNNCC 5 cut(s) 163, 370, 389, 615, 744
PspPI GGNCC 5 cut(s) 438, 499, 613, 639, 743
PspPPI RGGWCCY 1 cut(s) 613
PsuI RGATCY 1 cut(s) 368
RsaI GTAC 1 cut(s) 79
RsaNI GTAC 1 cut(s) 78
RseI CAYNNNNRTG 1 cut(s) 704
SacI GAGCTC 1 cut(s) 856
SalI GTCGAC 1 cut(s) 521
SaqAI TTAA 4 cut(s) 134, 357, 422, 824
SatI GCNGC 9 cut(s) 454, 538, 541, 621, 624, 712, 781, 948, 951
Sau3AI GATC 3 cut(s) 54, 368, 750
Sau96I GGNCC 5 cut(s) 438, 499, 613, 639, 743
ScaI AGTACT 1 cut(s) 79
SchI GAGTC 4 cut(s) 186, 555, 959, 970
ScrFI CCNGG 3 cut(s) 436, 611, 673
SduI GDGCHC 1 cut(s) 856
SfaNI GCATC 1 cut(s) 865
SgrDI CGTCGACG 1 cut(s) 521
SinI GGWCC 4 cut(s) 438, 499, 613, 639
SmiMI CAYNNNNRTG 1 cut(s) 704
Sse9I AATT 1 cut(s) 379
SspI AATATT 1 cut(s) 530
SspMI CTAG 1 cut(s) 174
SstI GAGCTC 1 cut(s) 856
StyD4I CCNGG 3 cut(s) 434, 609, 671
TaaI ACNGT 1 cut(s) 639
TaiI ACGT 9 cut(s) 299, 522, 632, 801, 813, 840, 870, 885, 896
TaqI TCGA 6 cut(s) 36, 99, 261, 312, 522, 786
TasI AATT 1 cut(s) 379
TatI WGTACW 1 cut(s) 77
TauI GCSGC 7 cut(s) 456, 543, 623, 626, 783, 950, 953
TfiI GAWTC 4 cut(s) 96, 646, 718, 759
Tru1I TTAA 4 cut(s) 134, 357, 422, 824
Tru9I TTAA 4 cut(s) 134, 357, 422, 824
TseFI GTSAC 2 cut(s) 833, 973
TseI GCWGC 2 cut(s) 537, 711
Tsp45I GTSAC 2 cut(s) 833, 973
TspDTI ATGAA 2 cut(s) 363, 581
TspGWI ACGGA 5 cut(s) 220, 277, 544, 846, 887
VpaK11BI GGWCC 4 cut(s) 438, 499, 613, 639
XmiI GTMKAC 2 cut(s) 522, 558
XspI CTAG 1 cut(s) 174
ZraI GACGTC 1 cut(s) 520
ZrmI AGTACT 1 cut(s) 79
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.