Rh5DG431200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0001093
Physical Location & Seq
Forward (+)
68969708 .. 68970028
321 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG431200.1

Sequence Viewer

Length: 321 bp
ATGCCGTCGCCGTACGAGTTGGGGTTGACGTTGGTGACGGAGAAGATGAAGCAGTGGAGAAAGAGGCGGGTGATTTGGTTCAGGGTCGAAGAATCGGACCGGGTTGTTGTAGATGACGGTGCCTGCACTGGCGGTGGGGACGGGGAGCTCACGGGAGAGGCCAACAACGCCGTTTGGGAGGTGGAAGTCGTGGAGGAAGGTGAGGTTGCGGAAGTTAAAAAGGAGGAGAAGTCGAAGCTGAGGTTCTCGGCTAGGGAGAAAGAAGAAGAGGGAGAGGAGAGAGAAAGCAAGAAGCTTTATTGGGTTCATGATATGGCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

106

Amino Acids

12.25

Weight (kDa)

4.82

Isoelectric Point (pI)

57.69

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 119
AciI CCGC 3 cut(s) 67, 132, 209
AfaI GTAC 1 cut(s) 14
AluBI AGCT 3 cut(s) 148, 238, 295
AluI AGCT 3 cut(s) 148, 238, 295
Alw21I GWGCWC 1 cut(s) 150
AoxI GGCC 1 cut(s) 159
AspS9I GGNCC 1 cut(s) 97
AsuC2I CCSGG 1 cut(s) 101
AsuHPI GGTGA 3 cut(s) 46, 82, 212
AvaII GGWCC 1 cut(s) 97
BanI GGYRCC 1 cut(s) 119
BanII GRGCYC 1 cut(s) 150
Bbv12I GWGCWC 1 cut(s) 150
BbvCI CCTCAGC 1 cut(s) 239
BceAI ACGGC 1 cut(s) 155
BcnI CCSGG 1 cut(s) 101
BfaI CTAG 1 cut(s) 252
Bme1390I CCNGG 1 cut(s) 101
Bme18I GGWCC 1 cut(s) 97
BmgT120I GGNCC 1 cut(s) 97
BmiI GGNNCC 1 cut(s) 121
BmrFI CCNGG 1 cut(s) 101
Bpu10I CCTNAGC 1 cut(s) 239
BpuMI CCSGG 1 cut(s) 101
BsaXI ACNNNNNCTCC 2 cut(s) 215, 245
Bse1I ACTGG 1 cut(s) 133
BseMII CTCAG 1 cut(s) 230
BseNI ACTGG 1 cut(s) 133
BseRI GAGGAG 2 cut(s) 239, 290
BsgI GTGCAG 1 cut(s) 109
BshFI GGCC 1 cut(s) 161
BshNI GGYRCC 1 cut(s) 119
BsiHKAI GWGCWC 1 cut(s) 150
BsiSI CCGG 1 cut(s) 100
BsiWI CGTACG 1 cut(s) 12
BslFI GGGAC 1 cut(s) 152
BsmFI GGGAC 1 cut(s) 152
BsnI GGCC 1 cut(s) 161
Bsp1286I GDGCHC 1 cut(s) 150
BspACI CCGC 3 cut(s) 67, 132, 209
BspANI GGCC 1 cut(s) 161
BspCNI CTCAG 1 cut(s) 231
BspHI TCATGA 1 cut(s) 307
BspLI GGNNCC 1 cut(s) 121
BspT107I GGYRCC 1 cut(s) 119
BsrI ACTGG 1 cut(s) 133
Bst4CI ACNGT 1 cut(s) 119
Bst6I CTCTTC 1 cut(s) 261
BstC8I GCNNGC 1 cut(s) 124
BstDEI CTNAG 1 cut(s) 239
BstMWI GCNNNNNNNGC 1 cut(s) 167
BstSCI CCNGG 1 cut(s) 99
BsuRI GGCC 1 cut(s) 161
BtsI GCAGTG 1 cut(s) 59
BtsIMutI CAGTG 2 cut(s) 59, 126
Cac8I GCNNGC 1 cut(s) 124
CciI TCATGA 1 cut(s) 307
Cfr13I GGNCC 1 cut(s) 97
CpoI CGGWCCG 1 cut(s) 97
Csp6I GTAC 1 cut(s) 13
CspI CGGWCCG 1 cut(s) 97
CviAII CATG 1 cut(s) 308
CviJI RGCY 5 cut(s) 148, 161, 238, 251, 295
CviKI_1 RGCY 5 cut(s) 148, 161, 238, 251, 295
CviQI GTAC 1 cut(s) 13
DdeI CTNAG 1 cut(s) 239
Eam1104I CTCTTC 1 cut(s) 261
EarI CTCTTC 1 cut(s) 261
Ecl136II GAGCTC 1 cut(s) 148
Eco24I GRGCYC 1 cut(s) 150
Eco47I GGWCC 1 cut(s) 97
Eco53kI GAGCTC 1 cut(s) 148
EcoICRI GAGCTC 1 cut(s) 148
EcoT38I GRGCYC 1 cut(s) 150
FaeI CATG 1 cut(s) 311
FaiI YATR 2 cut(s) 309, 314
FaqI GGGAC 1 cut(s) 152
FatI CATG 1 cut(s) 307
FauI CCCGC 1 cut(s) 60
FriOI GRGCYC 1 cut(s) 150
FspBI CTAG 1 cut(s) 252
HaeIII GGCC 1 cut(s) 161
HapII CCGG 1 cut(s) 100
Hin1II CATG 1 cut(s) 311
HincII GTYRAC 1 cut(s) 27
HindII GTYRAC 1 cut(s) 27
HindIII AAGCTT 1 cut(s) 293
HinfI GANTC 1 cut(s) 92
HpaII CCGG 1 cut(s) 100
HphI GGTGA 3 cut(s) 46, 82, 212
Hpy166II GTNNAC 1 cut(s) 27
Hpy188I TCNGA 1 cut(s) 97
Hpy188III TCNNGA 1 cut(s) 308
Hpy8I GTNNAC 1 cut(s) 27
Hpy99I CGWCG 1 cut(s) 10
HpyAV CCTTC 1 cut(s) 191
HpyCH4III ACNGT 1 cut(s) 119
HpyCH4IV ACGT 1 cut(s) 29
HpyCH4V TGCA 1 cut(s) 126
HpyF10VI GCNNNNNNNGC 1 cut(s) 167
HpyF3I CTNAG 1 cut(s) 239
HpySE526I ACGT 1 cut(s) 29
Hsp92II CATG 1 cut(s) 311
LmnI GCTCC 1 cut(s) 145
LpnPI CCDG 4 cut(s) 67, 113, 114, 136
MaeI CTAG 1 cut(s) 252
MaeII ACGT 1 cut(s) 29
MaeIII GTNAC 1 cut(s) 34
MboII GAAGA 4 cut(s) 55, 101, 275, 278
MhlI GDGCHC 1 cut(s) 150
MnlI CCTC 9 cut(s) 57, 151, 172, 187, 196, 217, 234, 262, 268
MseI TTAA 1 cut(s) 216
MspI CCGG 1 cut(s) 100
MspR9I CCNGG 1 cut(s) 101
MwoI GCNNNNNNNGC 1 cut(s) 167
NciI CCSGG 1 cut(s) 101
NlaIII CATG 1 cut(s) 311
NlaIV GGNNCC 1 cut(s) 121
NmeAIII GCCGAG 1 cut(s) 227
NmuCI GTSAC 1 cut(s) 34
PagI TCATGA 1 cut(s) 307
PfeI GAWTC 1 cut(s) 92
Pfl23II CGTACG 1 cut(s) 12
Psp124BI GAGCTC 1 cut(s) 150
PspLI CGTACG 1 cut(s) 12
PspN4I GGNNCC 1 cut(s) 121
PspPI GGNCC 1 cut(s) 97
RsaI GTAC 1 cut(s) 14
RsaNI GTAC 1 cut(s) 13
Rsr2I CGGWCCG 1 cut(s) 97
RsrII CGGWCCG 1 cut(s) 97
SacI GAGCTC 1 cut(s) 150
SaqAI TTAA 1 cut(s) 216
Sau96I GGNCC 1 cut(s) 97
ScrFI CCNGG 1 cut(s) 101
SduI GDGCHC 1 cut(s) 150
SetI ASST 8 cut(s) 32, 150, 183, 202, 207, 240, 245, 297
SinI GGWCC 1 cut(s) 97
SsiI CCGC 3 cut(s) 67, 132, 209
SspMI CTAG 1 cut(s) 252
SstI GAGCTC 1 cut(s) 150
StyD4I CCNGG 1 cut(s) 99
TaaI ACNGT 1 cut(s) 119
TaiI ACGT 1 cut(s) 32
TaqI TCGA 2 cut(s) 87, 233
TfiI GAWTC 1 cut(s) 92
Tru1I TTAA 1 cut(s) 216
Tru9I TTAA 1 cut(s) 216
TscAI CASTG 2 cut(s) 59, 133
TseFI GTSAC 1 cut(s) 34
Tsp45I GTSAC 1 cut(s) 34
TspDTI ATGAA 2 cut(s) 62, 296
TspGWI ACGGA 1 cut(s) 53
TspRI CASTG 2 cut(s) 59, 133
VpaK11BI GGWCC 1 cut(s) 97
XspI CTAG 1 cut(s) 252
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.