Rorug02G0398300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0001093
Physical Location & Seq
Reverse (-)
50871734 .. 50873242
1509 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0398300.1

Sequence Viewer

Length: 1509 bp
ATGGGCGAGCCTCCTCCAGCCTGCCCGTGGAGTTTTCTTCAGCCATTCTCTGACACGAGATCTCCCTCTTCTACATCTGAACAGAAAATTCACAAGGCAAAGACCTTTGCATCCATTGTTGCAGGTTCAGAGGAAACCCAAGTTCCTATAAGCCAACTACCAGCGCCAACAATTCGTGGAGACACGGTTTATGTGAAGATCAATGAGAGGATCTATCAAGAACAATTAGCAGCCTGCCGAAATAATTTGATCGGTCGATTGCTACTCCGTAAGGGCACTATTCCATTGAAAACTGAAACTTTCAAGGCTTCTCTGCATACTCTTTGGCGTCCGTTGGGTCCGTGGCGTCTGGTCCCCATTGGGAAAGGGTATTTTGACATTCACTTCAACTCTGAAGCAGATATGCGCAAAGTTTGGGGCGGTGGGACTTGCACTCTCAAATCTGGAATTTTTCGTCTGTCTCAATGGAAACCTGATTTCAGGCCTGGTGATGTGCTTCCCCAAACGCATGCACAAATCTGGGTACGTATTTCAGGCCTTAGTCAAGAGTATTGGCACCAACAACACATTCTTGAAATTGCTCGGGGAGTGGGCACCCCGTTGCAATTGGATGCGGCAACTAAGGAACAACGATATGGATATTATGCTAGAGTGTTGGTGGATGTTGATCTGTTGGGTGCTCTTCCTACGTCCATTATGGTTGAAAGAGAACACCTTTGTTTTCCAGTAGGTATTGAGTATGAAAACCTACCTCCTCAGTGTTCTCATTGTGGTTTGATAGGGCACGAAAGGAATAATTGCAGGCAACTAAAGTCCAAGGAAGTTCCTGCGAAACGTAAAGAGGTTCGTCAAGAATATCGTATAAAGAATAAATTGCAGGAAACCCATGTTCAAGAGGCTAGCTTCTCTGACGCACATGCCATTAACGATATTGACGGTACTATTGTTACTTTGAATGCCATAGAGCAACCTTTGGCAGGTGATCAAGTTCTCCATTCCGGTAATGATCAAGTGGCTTCACCTAATGAGATTTCCATTCAGGCTCCCATCCAAAGTGATGTTGTTGTGGCTCTTGTAAATACTGTTTTGGAGGAAGCAGCGGCAGAGGTTATGGAGGAACAAGCTCAAAGGGTAATGGAGGAGAATAATTCTAACCTAGCCTCTAATGATGCATCTGATGCTTCGGACACAGATATTAGTGATTCACCTACCCGTACTTGGGGGGATATTTTTGAGGAGGAGAGGATTGAAGGTTTGATCAGAGAGGCTCCTTCTAGTGCTCCACCACGCCTTGGTCCACCTCCTGGTTTTGTCAAATCTAATGTGGCTTCAGAAATTGATGCGGTTGCAAGATTTGTTAATGGTGAGGATATTGATGGGTTTATTCCGGTTCTCACTAAATCACAAAAGAAGTTGGAGAAAAAAAAACAAAAAACCGCTAACGCTTCTCCAGATCGTAAAACTCCATACCCATCAAGGGTTCGAAACAAGCCCCTCCGTCTTAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

502

Amino Acids

55.97

Weight (kDa)

6.49

Isoelectric Point (pI)

47.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4283 PF14111 78 - 162 8.3e-07 Domain of unknown function (DUF4283)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 968
Acc16I TGCGCA 1 cut(s) 407
Acc36I ACCTGC 2 cut(s) 113, 968
AccB1I GGYRCC 2 cut(s) 555, 593
AccB7I CCANNNNNTGG 2 cut(s) 1292, 1304
AciI CCGC 5 cut(s) 420, 614, 1100, 1343, 1437
AclWI GGATC 1 cut(s) 218
AcsI RAATTY 2 cut(s) 87, 447
AcuI CTGAAG 3 cut(s) 23, 414, 1314
AcyI GRCGYC 2 cut(s) 328, 346
AfaI GTAC 3 cut(s) 525, 940, 1216
AfiI CCNNNNNNNGG 7 cut(s) 27, 977, 1218, 1219, 1292, 1304, 1477
AgsI TTSAA 8 cut(s) 289, 304, 388, 575, 704, 893, 955, 1250
AjnI CCWGG 2 cut(s) 484, 1303
AjuI GAANNNNNNNTTGG 2 cut(s) 552, 584
AluBI AGCT 2 cut(s) 903, 1124
AluI AGCT 2 cut(s) 903, 1124
Alw21I GWGCWC 2 cut(s) 682, 1282
Alw26I GTCTC 2 cut(s) 174, 465
AlwI GGATC 1 cut(s) 218
Ama87I CYCGRG 1 cut(s) 582
AoxI GGCC 2 cut(s) 482, 535
ApeKI GCWGC 2 cut(s) 230, 1097
ApoI RAATTY 2 cut(s) 87, 447
AspLEI GCGC 2 cut(s) 166, 408
AspS9I GGNCC 3 cut(s) 338, 352, 1295
AsuHPI GGTGA 5 cut(s) 500, 992, 1011, 1197, 1376
AsuII TTCGAA 1 cut(s) 1483
AsuNHI GCTAGC 1 cut(s) 899
AvaI CYCGRG 1 cut(s) 582
AvaII GGWCC 3 cut(s) 338, 352, 1295
BaeGI GKGCMC 3 cut(s) 278, 596, 786
BaeI ACNNNNGTAYC 2 cut(s) 930, 963
BanI GGYRCC 2 cut(s) 555, 593
BauI CACGAG 1 cut(s) 55
Bbv12I GWGCWC 2 cut(s) 682, 1282
BbvI GCAGC 2 cut(s) 242, 1109
BccI CCATC 3 cut(s) 1055, 1370, 1480
BciT130I CCWGG 2 cut(s) 486, 1305
BclI TGATCA 3 cut(s) 982, 1006, 1257
BcoDI GTCTC 2 cut(s) 174, 465
BfaI CTAG 4 cut(s) 648, 900, 1157, 1275
BfoI RGCGCY 1 cut(s) 167
BfuAI ACCTGC 2 cut(s) 113, 968
BglII AGATCT 1 cut(s) 59
BisI GCNGC 4 cut(s) 231, 615, 1098, 1101
BlsI GCNGC 4 cut(s) 232, 616, 1099, 1102
Bme1390I CCNGG 2 cut(s) 486, 1305
Bme18I GGWCC 3 cut(s) 338, 352, 1295
BmeT110I CYCGRG 1 cut(s) 582
BmgT120I GGNCC 3 cut(s) 338, 352, 1295
BmiI GGNNCC 6 cut(s) 339, 354, 557, 595, 1044, 1269
BmrFI CCNGG 2 cut(s) 486, 1305
BmsI GCATC 6 cut(s) 119, 601, 1159, 1168, 1181, 1330
BmtI GCTAGC 1 cut(s) 903
BpmI CTGGAG 1 cut(s) 1434
Bpu14I TTCGAA 1 cut(s) 1483
BsaAI YACGTR 1 cut(s) 527
BsaBI GATNNNNATC 1 cut(s) 666
BsaHI GRCGYC 2 cut(s) 328, 346
BsaJI CCNNGG 4 cut(s) 26, 341, 816, 1291
BsaWI WCCGGW 2 cut(s) 998, 1387
BsaXI ACNNNNNCTCC 4 cut(s) 46, 76, 171, 201
Bsc4I CCNNNNNNNGG 7 cut(s) 27, 977, 1218, 1219, 1292, 1304, 1477
Bse1I ACTGG 1 cut(s) 725
Bse8I GATNNNNATC 1 cut(s) 666
BseBI CCWGG 2 cut(s) 486, 1305
BseDI CCNNGG 4 cut(s) 26, 341, 816, 1291
BseGI GGATG 4 cut(s) 110, 616, 667, 1047
BseJI GATNNNNATC 1 cut(s) 666
BseLI CCNNNNNNNGG 7 cut(s) 27, 977, 1218, 1219, 1292, 1304, 1477
BseMII CTCAG 1 cut(s) 770
BseNI ACTGG 1 cut(s) 725
BseRI GAGGAG 4 cut(s) 744, 1154, 1250, 1253
BseSI GKGCMC 3 cut(s) 278, 596, 786
BseXI GCAGC 2 cut(s) 242, 1109
Bsh1285I CGRYCG 1 cut(s) 256
BshFI GGCC 2 cut(s) 484, 537
BshNI GGYRCC 2 cut(s) 555, 593
BsiEI CGRYCG 1 cut(s) 256
BsiHKAI GWGCWC 2 cut(s) 682, 1282
BsiHKCI CYCGRG 1 cut(s) 582
BsiSI CCGG 2 cut(s) 999, 1388
BslFI GGGAC 2 cut(s) 338, 439
BslI CCNNNNNNNGG 7 cut(s) 27, 977, 1218, 1219, 1292, 1304, 1477
BsmAI GTCTC 2 cut(s) 174, 465
BsmFI GGGAC 2 cut(s) 338, 439
BsmI GAATGC 1 cut(s) 961
BsnI GGCC 2 cut(s) 484, 537
BsoBI CYCGRG 1 cut(s) 582
Bsp119I TTCGAA 1 cut(s) 1483
Bsp1286I GDGCHC 5 cut(s) 278, 596, 682, 786, 1282
Bsp143I GATC 9 cut(s) 59, 198, 210, 249, 667, 982, 1006, 1257, 1453
BspACI CCGC 5 cut(s) 420, 614, 1100, 1343, 1437
BspANI GGCC 2 cut(s) 484, 537
BspCNI CTCAG 1 cut(s) 769
BspLI GGNNCC 6 cut(s) 339, 354, 557, 595, 1044, 1269
BspMI ACCTGC 2 cut(s) 113, 968
BspOI GCTAGC 1 cut(s) 903
BspPI GGATC 1 cut(s) 218
BspQI GCTCTTC 1 cut(s) 687
BspT104I TTCGAA 1 cut(s) 1483
BspT107I GGYRCC 2 cut(s) 555, 593
BsrI ACTGG 1 cut(s) 725
BssECI CCNNGG 4 cut(s) 26, 341, 816, 1291
BssMI GATC 9 cut(s) 59, 198, 210, 249, 667, 982, 1006, 1257, 1453
BssNI GRCGYC 2 cut(s) 328, 346
BssSI CACGAG 1 cut(s) 55
BssT1I CCWWGG 2 cut(s) 816, 1291
Bst2BI CACGAG 1 cut(s) 55
Bst2UI CCWGG 2 cut(s) 486, 1305
Bst4CI ACNGT 3 cut(s) 187, 938, 1084
Bst6I CTCTTC 2 cut(s) 73, 687
BstACI GRCGYC 2 cut(s) 328, 346
BstAPI GCANNNNNTGC 1 cut(s) 1178
BstBAI YACGTR 1 cut(s) 527
BstBI TTCGAA 1 cut(s) 1483
BstC8I GCNNGC 6 cut(s) 8, 22, 235, 510, 803, 901
BstDEI CTNAG 4 cut(s) 539, 621, 756, 1502
BstDSI CCRYGG 2 cut(s) 26, 341
BstENI CCTNNNNNAGG 1 cut(s) 975
BstF5I GGATG 4 cut(s) 110, 616, 667, 1047
BstH2I RGCGCY 1 cut(s) 167
BstHHI GCGC 2 cut(s) 166, 408
BstKTI GATC 9 cut(s) 62, 201, 213, 252, 670, 985, 1009, 1260, 1456
BstMAI GTCTC 2 cut(s) 174, 465
BstMBI GATC 9 cut(s) 59, 198, 210, 249, 667, 982, 1006, 1257, 1453
BstMCI CGRYCG 1 cut(s) 256
BstMWI GCNNNNNNNGC 1 cut(s) 1178
BstNI CCWGG 2 cut(s) 486, 1305
BstNSI RCATGY 2 cut(s) 512, 920
BstSCI CCNGG 2 cut(s) 484, 1303
BstSLI GKGCMC 3 cut(s) 278, 596, 786
BstSNI TACGTA 1 cut(s) 527
BstV1I GCAGC 2 cut(s) 242, 1109
BstX2I RGATCY 2 cut(s) 59, 210
BstYI RGATCY 2 cut(s) 59, 210
BsuRI GGCC 2 cut(s) 484, 537
BtgI CCRYGG 2 cut(s) 26, 341
BtsCI GGATG 4 cut(s) 110, 616, 667, 1047
BtsIMutI CAGTG 1 cut(s) 764
BveI ACCTGC 2 cut(s) 113, 968
Cac8I GCNNGC 6 cut(s) 8, 22, 235, 510, 803, 901
CfoI GCGC 2 cut(s) 166, 408
Cfr13I GGNCC 3 cut(s) 338, 352, 1295
CseI GACGC 3 cut(s) 317, 335, 920
Csp6I GTAC 3 cut(s) 524, 939, 1215
CviAII CATG 3 cut(s) 509, 887, 917
CviQI GTAC 3 cut(s) 524, 939, 1215
DdeI CTNAG 4 cut(s) 539, 621, 756, 1502
DpnI GATC 9 cut(s) 61, 200, 212, 251, 669, 984, 1008, 1259, 1455
DpnII GATC 9 cut(s) 59, 198, 210, 249, 667, 982, 1006, 1257, 1453
Eam1104I CTCTTC 2 cut(s) 73, 687
EarI CTCTTC 2 cut(s) 73, 687
Eco105I TACGTA 1 cut(s) 527
Eco130I CCWWGG 2 cut(s) 816, 1291
Eco147I AGGCCT 2 cut(s) 484, 537
Eco47I GGWCC 3 cut(s) 338, 352, 1295
Eco57I CTGAAG 3 cut(s) 23, 414, 1314
Eco88I CYCGRG 1 cut(s) 582
EcoNI CCTNNNNNAGG 1 cut(s) 975
EcoRII CCWGG 2 cut(s) 484, 1303
EcoT14I CCWWGG 2 cut(s) 816, 1291
EcoT22I ATGCAT 1 cut(s) 1174
ErhI CCWWGG 2 cut(s) 816, 1291
FaeI CATG 3 cut(s) 512, 890, 920
FaqI GGGAC 2 cut(s) 338, 439
FatI CATG 3 cut(s) 508, 886, 916
FbaI TGATCA 3 cut(s) 982, 1006, 1257
Fnu4HI GCNGC 4 cut(s) 231, 615, 1098, 1101
FokI GGATG 4 cut(s) 97, 623, 674, 1034
Fsp4HI GCNGC 4 cut(s) 231, 615, 1098, 1101
FspBI CTAG 4 cut(s) 648, 900, 1157, 1275
FspI TGCGCA 1 cut(s) 407
GlaI GCGC 2 cut(s) 165, 407
GluI GCNGC 4 cut(s) 231, 615, 1098, 1101
GsuI CTGGAG 1 cut(s) 1434
HaeII RGCGCY 1 cut(s) 167
HaeIII GGCC 2 cut(s) 484, 537
HapII CCGG 2 cut(s) 999, 1388
HgaI GACGC 3 cut(s) 317, 335, 920
HhaI GCGC 2 cut(s) 166, 408
Hin1I GRCGYC 2 cut(s) 328, 346
Hin1II CATG 3 cut(s) 512, 890, 920
Hin6I GCGC 2 cut(s) 164, 406
HinP1I GCGC 2 cut(s) 164, 406
HinfI GANTC 1 cut(s) 1202
HpaII CCGG 2 cut(s) 999, 1388
HphI GGTGA 5 cut(s) 500, 992, 1011, 1197, 1376
Hpy166II GTNNAC 1 cut(s) 1298
Hpy188I TCNGA 9 cut(s) 52, 79, 130, 394, 910, 1177, 1186, 1262, 1333
Hpy188III TCNNGA 7 cut(s) 218, 444, 545, 572, 851, 893, 1451
Hpy8I GTNNAC 1 cut(s) 1298
HpyAV CCTTC 2 cut(s) 1244, 1281
HpyCH4III ACNGT 3 cut(s) 187, 938, 1084
HpyCH4IV ACGT 3 cut(s) 526, 689, 835
HpyF10VI GCNNNNNNNGC 1 cut(s) 1178
HpyF3I CTNAG 4 cut(s) 539, 621, 756, 1502
HpySE526I ACGT 3 cut(s) 526, 689, 835
Hsp92I GRCGYC 2 cut(s) 328, 346
Hsp92II CATG 3 cut(s) 512, 890, 920
HspAI GCGC 2 cut(s) 164, 406
Ksp22I TGATCA 3 cut(s) 982, 1006, 1257
Kzo9I GATC 9 cut(s) 59, 198, 210, 249, 667, 982, 1006, 1257, 1453
LguI GCTCTTC 1 cut(s) 687
LmnI GCTCC 3 cut(s) 1048, 1273, 1285
Lsp1109I GCAGC 2 cut(s) 242, 1109
LweI GCATC 6 cut(s) 119, 601, 1159, 1168, 1181, 1330
MaeI CTAG 4 cut(s) 648, 900, 1157, 1275
MaeII ACGT 3 cut(s) 526, 689, 835
MaeIII GTNAC 1 cut(s) 946
MalI GATC 9 cut(s) 61, 200, 212, 251, 669, 984, 1008, 1259, 1455
MboI GATC 9 cut(s) 59, 198, 210, 249, 667, 982, 1006, 1257, 1453
MboII GAAGA 4 cut(s) 29, 60, 208, 674
MfeI CAATTG 1 cut(s) 605
MflI RGATCY 2 cut(s) 59, 210
MhlI GDGCHC 5 cut(s) 278, 596, 682, 786, 1282
MmeI TCCRAC 1 cut(s) 1395
Mph1103I ATGCAT 1 cut(s) 1174
MseI TTAA 2 cut(s) 924, 1359
MspA1I CMGCKG 1 cut(s) 1100
MspI CCGG 2 cut(s) 999, 1388
MspR9I CCNGG 2 cut(s) 486, 1305
MunI CAATTG 1 cut(s) 605
Mva1269I GAATGC 1 cut(s) 961
MvaI CCWGG 2 cut(s) 486, 1305
MwoI GCNNNNNNNGC 1 cut(s) 1178
NdeII GATC 9 cut(s) 59, 198, 210, 249, 667, 982, 1006, 1257, 1453
NheI GCTAGC 1 cut(s) 899
NlaIII CATG 3 cut(s) 512, 890, 920
NlaIV GGNNCC 6 cut(s) 339, 354, 557, 595, 1044, 1269
NsbI TGCGCA 1 cut(s) 407
NsiI ATGCAT 1 cut(s) 1174
NspI RCATGY 2 cut(s) 512, 920
NspV TTCGAA 1 cut(s) 1483
PaeI GCATGC 1 cut(s) 512
PaqCI CACCTGC 1 cut(s) 968
PceI AGGCCT 2 cut(s) 484, 537
PciSI GCTCTTC 1 cut(s) 687
PctI GAATGC 1 cut(s) 961
PfeI GAWTC 1 cut(s) 1202
PflMI CCANNNNNTGG 2 cut(s) 1292, 1304
PkrI GCNGC 4 cut(s) 232, 616, 1099, 1102
Ppu21I YACGTR 1 cut(s) 527
Psp6I CCWGG 2 cut(s) 484, 1303
PspGI CCWGG 2 cut(s) 484, 1303
PspN4I GGNNCC 6 cut(s) 339, 354, 557, 595, 1044, 1269
PspPI GGNCC 3 cut(s) 338, 352, 1295
PsuI RGATCY 2 cut(s) 59, 210
RsaI GTAC 3 cut(s) 525, 940, 1216
RsaNI GTAC 3 cut(s) 524, 939, 1215
SapI GCTCTTC 1 cut(s) 687
SaqAI TTAA 2 cut(s) 924, 1359
SatI GCNGC 4 cut(s) 231, 615, 1098, 1101
Sau3AI GATC 9 cut(s) 59, 198, 210, 249, 667, 982, 1006, 1257, 1453
Sau96I GGNCC 3 cut(s) 338, 352, 1295
ScrFI CCNGG 2 cut(s) 486, 1305
SduI GDGCHC 5 cut(s) 278, 596, 682, 786, 1282
SfaNI GCATC 6 cut(s) 119, 601, 1159, 1168, 1181, 1330
SfuI TTCGAA 1 cut(s) 1483
SinI GGWCC 3 cut(s) 338, 352, 1295
SnaBI TACGTA 1 cut(s) 527
SphI GCATGC 1 cut(s) 512
SseBI AGGCCT 2 cut(s) 484, 537
SsiI CCGC 5 cut(s) 420, 614, 1100, 1343, 1437
SspMI CTAG 4 cut(s) 648, 900, 1157, 1275
StuI AGGCCT 2 cut(s) 484, 537
StyD4I CCNGG 2 cut(s) 484, 1303
StyI CCWWGG 2 cut(s) 816, 1291
TaaI ACNGT 3 cut(s) 187, 938, 1084
TaiI ACGT 3 cut(s) 529, 692, 838
TaqI TCGA 2 cut(s) 256, 1483
TaqII GACCGA 1 cut(s) 242
TauI GCSGC 2 cut(s) 617, 1103
TfiI GAWTC 1 cut(s) 1202
Tru1I TTAA 2 cut(s) 924, 1359
Tru9I TTAA 2 cut(s) 924, 1359
TscAI CASTG 1 cut(s) 764
TseI GCWGC 2 cut(s) 230, 1097
TspDTI ATGAA 1 cut(s) 756
TspGWI ACGGA 4 cut(s) 257, 321, 330, 1487
TspRI CASTG 1 cut(s) 764
Van91I CCANNNNNTGG 2 cut(s) 1292, 1304
VpaK11BI GGWCC 3 cut(s) 338, 352, 1295
XagI CCTNNNNNAGG 1 cut(s) 975
XapI RAATTY 2 cut(s) 87, 447
XceI RCATGY 2 cut(s) 512, 920
XcmI CCANNNNNNNNNTGG 1 cut(s) 24
XspI CTAG 4 cut(s) 648, 900, 1157, 1275
Zsp2I ATGCAT 1 cut(s) 1174
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.