MD13G1040500.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0001093
Physical Location & Seq
Forward (+)
2751216 .. 2752127
912 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1040500.v1.1.491

Sequence Viewer

Length: 912 bp
ATGGAAATTCCGGTGCTCAACATAATTACTGATCTTCCGTCCCGCCTGACCTCGCTGCCGGACCCATCTTTTCTATGGCAGAGTTTGTCGTTTCCCGGAATCCAAAACGTCTCTCAAGCTTACAGTTTATGGACATGGGGAGCTCTAGTCCTCGCAATTCTAGCTTCCTTCGGCACCCTCATCGCTATAATCAACATTTTTGTCATCGGTCTCCGCCGCCTCCGGATATTAGACCCGCAGCCGCTTAATCAGCAGCTCCACGACGGCGAGTATTCCGACGTCAGTGACGACTACGATATCTGCTCTGCTTCTTCGGCGTCCTCCTTATCCGATGAAGATTTGGAACCCGAAATATCGTTCGACGACGAAAGTAATTCGATTGGGAGAGATGAACAATTCCACGTCAAAGGCTCGGGTTATTACGGCGACGGCGGAGTGCAGAATCGTAATTTTCGACTCCTCCGCCGCCGAAGCTTCGGAGGACATCGATTCTCGTGGTCCGACTTCACGGCGGGCAATAGCGTCGTAAAGCTCTGGGACACATCGTCGCCGGCCACGTTTTTGTCCGCGGACCCAGCCCCGTCGGGGCTCGTGTCTCTGGGTGTCTGGGACGCGCGCGTCGGATGCCGGATTCCGGCGATACTCGCGGAGTGGGGCCCGCAGATCGGGAGAATAGTTGGAGTGGCATCTGGTGGCGTGGAGAAAGTCTACGTCAAGGATGGCGTAACCGGCCATTTGACGGTCGGTGACGTGAGGAAAGTGGCGTCGCCGTTAGGGAGTGTAACGGAGGCTGACGTGGACGATACGTGGTGGGACGCCGACGCCGTTGTTGTGACGGACGAATCAGCGGTGAGTGGATGTGACTCGGCCGTGATACGGTGTTGTAGCGCGGTTAGGTCTTATCTGCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

304

Amino Acids

32.75

Weight (kDa)

4.51

Isoelectric Point (pI)

42.72

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 617
AatII GACGTC 1 cut(s) 282
AccB1I GGYRCC 1 cut(s) 173
AccI GTMKAC 1 cut(s) 708
AccII CGCG 6 cut(s) 569, 614, 616, 618, 647, 890
AccIII TCCGGA 1 cut(s) 222
AcoI YGGCCR 3 cut(s) 552, 730, 867
AcsI RAATTY 1 cut(s) 6
AcyI GRCGYC 5 cut(s) 279, 317, 764, 816, 822
AfiI CCNNNNNNNGG 5 cut(s) 585, 634, 665, 739, 876
AhdI GACNNNNNGTC 1 cut(s) 544
AjiI CACGTC 3 cut(s) 403, 751, 796
AluBI AGCT 6 cut(s) 119, 143, 164, 256, 474, 532
AluI AGCT 6 cut(s) 119, 143, 164, 256, 474, 532
Alw21I GWGCWC 2 cut(s) 18, 145
Alw26I GTCTC 3 cut(s) 115, 215, 600
Ama87I CYCGRG 1 cut(s) 412
Aor13HI TCCGGA 1 cut(s) 222
AoxI GGCC 4 cut(s) 552, 655, 730, 867
ApaI GGGCCC 1 cut(s) 659
ApeKI GCWGC 3 cut(s) 55, 238, 253
ApoI RAATTY 1 cut(s) 6
AspLEI GCGC 3 cut(s) 616, 618, 890
AspS9I GGNCC 5 cut(s) 61, 498, 571, 655, 656
AsuC2I CCSGG 1 cut(s) 96
AsuHPI GGTGA 2 cut(s) 758, 862
AvaI CYCGRG 1 cut(s) 412
AvaII GGWCC 3 cut(s) 61, 498, 571
BaeGI GKGCMC 1 cut(s) 659
BanI GGYRCC 1 cut(s) 173
BanII GRGCYC 3 cut(s) 145, 591, 659
BauI CACGAG 2 cut(s) 493, 590
Bbv12I GWGCWC 2 cut(s) 18, 145
BbvI GCAGC 3 cut(s) 42, 250, 265
BccI CCATC 2 cut(s) 73, 713
BceAI ACGGC 7 cut(s) 280, 439, 445, 525, 754, 809, 854
BcgI CGANNNNNNTGC 4 cut(s) 163, 197, 505, 539
BcnI CCSGG 1 cut(s) 96
BcoDI GTCTC 3 cut(s) 115, 215, 600
BfaI CTAG 2 cut(s) 146, 161
BisI GCNGC 6 cut(s) 56, 217, 239, 242, 254, 466
BlsI GCNGC 6 cut(s) 57, 218, 240, 243, 255, 467
Bme1390I CCNGG 1 cut(s) 96
Bme18I GGWCC 3 cut(s) 61, 498, 571
BmeRI GACNNNNNGTC 1 cut(s) 544
BmeT110I CYCGRG 1 cut(s) 412
BmgBI CACGTC 3 cut(s) 403, 751, 796
BmgT120I GGNCC 5 cut(s) 61, 498, 571, 655, 656
BmiI GGNNCC 6 cut(s) 63, 175, 345, 573, 656, 657
BmrFI CCNGG 1 cut(s) 96
BmsI GCATC 2 cut(s) 614, 695
BpuEI CTTGAG 1 cut(s) 99
BpuMI CCSGG 1 cut(s) 96
Bsa29I ATCGAT 1 cut(s) 487
BsaAI YACGTR 1 cut(s) 807
BsaHI GRCGYC 5 cut(s) 279, 317, 764, 816, 822
BsaI GGTCTC 1 cut(s) 215
BsaJI CCNNGG 1 cut(s) 567
BsaWI WCCGGW 2 cut(s) 10, 222
BsaXI ACNNNNNCTCC 2 cut(s) 132, 162
Bsc4I CCNNNNNNNGG 5 cut(s) 585, 634, 665, 739, 876
Bse118I RCCGGY 2 cut(s) 550, 728
BseAI TCCGGA 1 cut(s) 222
BseCI ATCGAT 1 cut(s) 487
BseDI CCNNGG 1 cut(s) 567
BseGI GGATG 3 cut(s) 629, 724, 863
BseLI CCNNNNNNNGG 5 cut(s) 585, 634, 665, 739, 876
BsePI GCGCGC 1 cut(s) 614
BseRI GAGGAG 1 cut(s) 449
BseSI GKGCMC 1 cut(s) 659
BseX3I CGGCCG 1 cut(s) 867
BseXI GCAGC 3 cut(s) 42, 250, 265
BseYI CCCAGC 1 cut(s) 574
BsgI GTGCAG 1 cut(s) 458
Bsh1236I CGCG 6 cut(s) 569, 614, 616, 618, 647, 890
Bsh1285I CGRYCG 2 cut(s) 744, 870
BshFI GGCC 4 cut(s) 554, 657, 732, 869
BshNI GGYRCC 1 cut(s) 173
BshVI ATCGAT 1 cut(s) 487
BsiEI CGRYCG 2 cut(s) 744, 870
BsiHKAI GWGCWC 2 cut(s) 18, 145
BsiHKCI CYCGRG 1 cut(s) 412
BsiSI CCGG 8 cut(s) 11, 59, 96, 223, 551, 628, 635, 729
BslFI GGGAC 4 cut(s) 25, 551, 623, 827
BslI CCNNNNNNNGG 5 cut(s) 585, 634, 665, 739, 876
BsmAI GTCTC 3 cut(s) 115, 215, 600
BsmBI CGTCTC 1 cut(s) 115
BsmFI GGGAC 4 cut(s) 25, 551, 623, 827
BsnI GGCC 4 cut(s) 554, 657, 732, 869
Bso31I GGTCTC 1 cut(s) 215
BsoBI CYCGRG 1 cut(s) 412
Bsp120I GGGCCC 1 cut(s) 655
Bsp1286I GDGCHC 4 cut(s) 18, 145, 591, 659
Bsp13I TCCGGA 1 cut(s) 222
Bsp143I GATC 2 cut(s) 31, 663
BspANI GGCC 4 cut(s) 554, 657, 732, 869
BspDI ATCGAT 1 cut(s) 487
BspEI TCCGGA 1 cut(s) 222
BspFNI CGCG 6 cut(s) 569, 614, 616, 618, 647, 890
BspLI GGNNCC 6 cut(s) 63, 175, 345, 573, 656, 657
BspT107I GGYRCC 1 cut(s) 173
BspTNI GGTCTC 1 cut(s) 215
BsrFI RCCGGY 2 cut(s) 550, 728
BssAI RCCGGY 2 cut(s) 550, 728
BssECI CCNNGG 1 cut(s) 567
BssHII GCGCGC 1 cut(s) 614
BssMI GATC 2 cut(s) 31, 663
BssNI GRCGYC 5 cut(s) 279, 317, 764, 816, 822
BssSI CACGAG 2 cut(s) 493, 590
Bst2BI CACGAG 2 cut(s) 493, 590
Bst4CI ACNGT 3 cut(s) 125, 742, 879
BstACI GRCGYC 5 cut(s) 279, 317, 764, 816, 822
BstBAI YACGTR 1 cut(s) 807
BstC8I GCNNGC 4 cut(s) 514, 552, 616, 659
BstDSI CCRYGG 1 cut(s) 567
BstF5I GGATG 3 cut(s) 629, 724, 863
BstFNI CGCG 6 cut(s) 569, 614, 616, 618, 647, 890
BstHHI GCGC 3 cut(s) 616, 618, 890
BstKTI GATC 2 cut(s) 34, 666
BstMAI GTCTC 3 cut(s) 115, 215, 600
BstMBI GATC 2 cut(s) 31, 663
BstMCI CGRYCG 2 cut(s) 744, 870
BstMWI GCNNNNNNNGC 8 cut(s) 161, 250, 314, 471, 575, 624, 644, 729
BstSCI CCNGG 1 cut(s) 94
BstSLI GKGCMC 1 cut(s) 659
BstUI CGCG 6 cut(s) 569, 614, 616, 618, 647, 890
BstV1I GCAGC 3 cut(s) 42, 250, 265
BstZI CGGCCG 1 cut(s) 867
Bsu15I ATCGAT 1 cut(s) 487
BsuRI GGCC 4 cut(s) 554, 657, 732, 869
BsuTUI ATCGAT 1 cut(s) 487
BtgI CCRYGG 1 cut(s) 567
BtgZI GCGATG 1 cut(s) 166
BtrI CACGTC 3 cut(s) 403, 751, 796
BtsCI GGATG 3 cut(s) 629, 724, 863
BtsIMutI CAGTG 1 cut(s) 289
Cac8I GCNNGC 4 cut(s) 514, 552, 616, 659
CfoI GCGC 3 cut(s) 616, 618, 890
Cfr10I RCCGGY 2 cut(s) 550, 728
Cfr13I GGNCC 5 cut(s) 61, 498, 571, 655, 656
Cfr42I CCGCGG 1 cut(s) 570
ClaI ATCGAT 1 cut(s) 487
CseI GACGC 7 cut(s) 306, 511, 607, 620, 753, 824, 830
CviAII CATG 1 cut(s) 135
DpnI GATC 2 cut(s) 33, 665
DpnII GATC 2 cut(s) 31, 663
DrdI GACNNNNNNGTC 1 cut(s) 617
DriI GACNNNNNGTC 1 cut(s) 544
DseDI GACNNNNNNGTC 1 cut(s) 617
EaeI YGGCCR 3 cut(s) 552, 730, 867
EagI CGGCCG 1 cut(s) 867
Eam1105I GACNNNNNGTC 1 cut(s) 544
EciI GGCGGA 3 cut(s) 203, 447, 452
Ecl136II GAGCTC 1 cut(s) 143
EclXI CGGCCG 1 cut(s) 867
Eco24I GRGCYC 3 cut(s) 145, 591, 659
Eco31I GGTCTC 1 cut(s) 215
Eco32I GATATC 1 cut(s) 298
Eco47I GGWCC 3 cut(s) 61, 498, 571
Eco52I CGGCCG 1 cut(s) 867
Eco53kI GAGCTC 1 cut(s) 143
Eco88I CYCGRG 1 cut(s) 412
EcoICRI GAGCTC 1 cut(s) 143
EcoO109I RGGNCCY 1 cut(s) 655
EcoRV GATATC 1 cut(s) 298
EcoT38I GRGCYC 3 cut(s) 145, 591, 659
Esp3I CGTCTC 1 cut(s) 115
FaeI CATG 1 cut(s) 138
FaiI YATR 5 cut(s) 23, 76, 130, 136, 188
FaqI GGGAC 4 cut(s) 25, 551, 623, 827
FatI CATG 1 cut(s) 134
FauI CCCGC 4 cut(s) 50, 243, 505, 666
FblI GTMKAC 1 cut(s) 708
Fnu4HI GCNGC 6 cut(s) 56, 217, 239, 242, 254, 466
FokI GGATG 3 cut(s) 636, 731, 870
FriOI GRGCYC 3 cut(s) 145, 591, 659
Fsp4HI GCNGC 6 cut(s) 56, 217, 239, 242, 254, 466
FspBI CTAG 2 cut(s) 146, 161
GlaI GCGC 3 cut(s) 615, 617, 889
GluI GCNGC 6 cut(s) 56, 217, 239, 242, 254, 466
GsaI CCCAGC 1 cut(s) 578
HaeIII GGCC 4 cut(s) 554, 657, 732, 869
HapII CCGG 8 cut(s) 11, 59, 96, 223, 551, 628, 635, 729
HgaI GACGC 7 cut(s) 306, 511, 607, 620, 753, 824, 830
HhaI GCGC 3 cut(s) 616, 618, 890
Hin1I GRCGYC 5 cut(s) 279, 317, 764, 816, 822
Hin1II CATG 1 cut(s) 138
Hin6I GCGC 3 cut(s) 614, 616, 888
HinP1I GCGC 3 cut(s) 614, 616, 888
HindIII AAGCTT 2 cut(s) 117, 472
HinfI GANTC 7 cut(s) 99, 442, 456, 489, 631, 842, 863
HpaII CCGG 8 cut(s) 11, 59, 96, 223, 551, 628, 635, 729
HphI GGTGA 2 cut(s) 758, 862
Hpy166II GTNNAC 2 cut(s) 709, 799
Hpy188I TCNGA 5 cut(s) 277, 331, 479, 502, 623
Hpy188III TCNNGA 2 cut(s) 223, 667
Hpy8I GTNNAC 2 cut(s) 709, 799
HpyAV CCTTC 1 cut(s) 178
HpyCH4III ACNGT 3 cut(s) 125, 742, 879
HpyCH4IV ACGT 8 cut(s) 108, 279, 402, 557, 711, 750, 795, 806
HpyCH4V TGCA 1 cut(s) 439
HpyF10VI GCNNNNNNNGC 8 cut(s) 161, 250, 314, 471, 575, 624, 644, 729
HpySE526I ACGT 8 cut(s) 108, 279, 402, 557, 711, 750, 795, 806
Hsp92I GRCGYC 5 cut(s) 279, 317, 764, 816, 822
Hsp92II CATG 1 cut(s) 138
HspAI GCGC 3 cut(s) 614, 616, 888
Kpn2I TCCGGA 1 cut(s) 222
KroI GCCGGC 1 cut(s) 550
KroNI GCCGGC 1 cut(s) 552
KspI CCGCGG 1 cut(s) 570
Kzo9I GATC 2 cut(s) 31, 663
LmnI GCTCC 2 cut(s) 140, 261
Lsp1109I GCAGC 3 cut(s) 42, 250, 265
LweI GCATC 2 cut(s) 614, 695
MaeI CTAG 2 cut(s) 146, 161
MaeII ACGT 8 cut(s) 108, 279, 402, 557, 711, 750, 795, 806
MaeIII GTNAC 6 cut(s) 284, 724, 746, 781, 832, 860
MalI GATC 2 cut(s) 33, 665
MauBI CGCGCGCG 1 cut(s) 614
MboI GATC 2 cut(s) 31, 663
MboII GAAGA 3 cut(s) 26, 303, 347
MhlI GDGCHC 4 cut(s) 18, 145, 591, 659
MluCI AATT 6 cut(s) 6, 24, 156, 373, 395, 448
MlyI GAGTC 2 cut(s) 450, 857
MmeI TCCRAC 4 cut(s) 300, 525, 601, 658
MnlI CCTC 9 cut(s) 61, 161, 188, 230, 331, 470, 473, 747, 781
MroI TCCGGA 1 cut(s) 222
MroNI GCCGGC 1 cut(s) 550
MseI TTAA 1 cut(s) 246
MspA1I CMGCKG 2 cut(s) 569, 848
MspI CCGG 8 cut(s) 11, 59, 96, 223, 551, 628, 635, 729
MspR9I CCNGG 1 cut(s) 96
MvnI CGCG 6 cut(s) 569, 614, 616, 618, 647, 890
MwoI GCNNNNNNNGC 8 cut(s) 161, 250, 314, 471, 575, 624, 644, 729
NaeI GCCGGC 1 cut(s) 552
NciI CCSGG 1 cut(s) 96
NdeII GATC 2 cut(s) 31, 663
NgoMIV GCCGGC 1 cut(s) 550
NlaIII CATG 1 cut(s) 138
NlaIV GGNNCC 6 cut(s) 63, 175, 345, 573, 656, 657
NmeAIII GCCGAG 1 cut(s) 845
NmuCI GTSAC 4 cut(s) 284, 746, 832, 860
PauI GCGCGC 1 cut(s) 614
PcsI WCGNNNNNNNCGW 4 cut(s) 285, 451, 554, 822
PdiI GCCGGC 1 cut(s) 552
PfeI GAWTC 5 cut(s) 99, 442, 489, 631, 842
PfoI TCCNGGA 1 cut(s) 94
PkrI GCNGC 6 cut(s) 57, 218, 240, 243, 255, 467
PleI GAGTC 2 cut(s) 450, 857
PpsI GAGTC 2 cut(s) 450, 857
Ppu21I YACGTR 1 cut(s) 807
Psp124BI GAGCTC 1 cut(s) 145
PspFI CCCAGC 1 cut(s) 574
PspN4I GGNNCC 6 cut(s) 63, 175, 345, 573, 656, 657
PspOMI GGGCCC 1 cut(s) 655
PspPI GGNCC 5 cut(s) 61, 498, 571, 655, 656
PteI GCGCGC 1 cut(s) 614
SacI GAGCTC 1 cut(s) 145
SacII CCGCGG 1 cut(s) 570
SaqAI TTAA 1 cut(s) 246
SatI GCNGC 6 cut(s) 56, 217, 239, 242, 254, 466
Sau3AI GATC 2 cut(s) 31, 663
Sau96I GGNCC 5 cut(s) 61, 498, 571, 655, 656
SchI GAGTC 2 cut(s) 450, 857
ScrFI CCNGG 1 cut(s) 96
SduI GDGCHC 4 cut(s) 18, 145, 591, 659
SfaNI GCATC 2 cut(s) 614, 695
Sfr303I CCGCGG 1 cut(s) 570
SgrBI CCGCGG 1 cut(s) 570
SinI GGWCC 3 cut(s) 61, 498, 571
SmlI CTYRAG 1 cut(s) 114
SmoI CTYRAG 1 cut(s) 114
Sse9I AATT 6 cut(s) 6, 24, 156, 373, 395, 448
SspMI CTAG 2 cut(s) 146, 161
SstI GAGCTC 1 cut(s) 145
StyD4I CCNGG 1 cut(s) 94
TaaI ACNGT 3 cut(s) 125, 742, 879
TaiI ACGT 8 cut(s) 111, 282, 405, 560, 714, 753, 798, 809
TaqI TCGA 4 cut(s) 360, 377, 454, 487
TaqII GACCGA 1 cut(s) 197
TasI AATT 6 cut(s) 6, 24, 156, 373, 395, 448
TauI GCSGC 3 cut(s) 219, 244, 468
TfiI GAWTC 5 cut(s) 99, 442, 489, 631, 842
Tru1I TTAA 1 cut(s) 246
Tru9I TTAA 1 cut(s) 246
TscAI CASTG 1 cut(s) 289
TseFI GTSAC 4 cut(s) 284, 746, 832, 860
TseI GCWGC 3 cut(s) 55, 238, 253
Tsp45I GTSAC 4 cut(s) 284, 746, 832, 860
TspDTI ATGAA 2 cut(s) 348, 405
TspGWI ACGGA 3 cut(s) 27, 800, 851
TspRI CASTG 1 cut(s) 289
VpaK11BI GGWCC 3 cut(s) 61, 498, 571
XapI RAATTY 1 cut(s) 6
XcmI CCANNNNNNNNNTGG 1 cut(s) 72
XmiI GTMKAC 1 cut(s) 708
XspI CTAG 2 cut(s) 146, 161
ZraI GACGTC 1 cut(s) 280
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.