MD16G1041500.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0001093
Physical Location & Seq
Forward (+)
2921465 .. 2922388
924 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1041500.v1.1.491

Sequence Viewer

Length: 924 bp
ATGGAAATCCCAGTGCTCAATAGAATTACAGATCTTCCTTCCCGCCTGACCTCCTTGCCGGACCCATCTTTTCTTTCCCAGAGTTTATCGATTCCCGGAATCCAAAACGTCTCTCAAGCTTACAGTTTCTGGAAATGGGGAGCTCTAATCCTCGCAATTCTTGCTTCCTTCGGCACCCTCATCACTAAAATCAACATTTTTGCCATCAGTCTCCGCCGCCTCCGGTTATTGGCCCCGCAGCCGCTTTCCCAGCAGCGCCACGACGGCGATTACTCCGACGTCAGCGATGACGACGATATCTGCTCTGTTTCGTCAGCTTCCTCCGTATCCGATGACGAATCGGAATCCGAAATATCGTACGACGACGAAAGCAGAACGATTGGGAGAGATGAACATTTCCACGTCAGAGGCTCTGGGTATTACGGCGACGACGGAGAGCAGAATCGTAACTTGGGACTCCGCCTCCGCAGAAGCTTCGGAGGCCATCGATTCTCATGGTCTGACTTCGCGGCCGGCAAAAGCGTCGTCAAGCTCTGGGACTCGTCGTCTCCGTCTACGTTTCTGTCTGCTGACTCGGGCGTGTCGGGGCGCGTGTCGCTAGGTGTTTGGGACGCGCGCGTCGGATGTCGGATTCCGGCGATACTCGCTGATTGGGGCCCGCAGCTCGGGAGAATGGTTGGAGTGGCGTCAGGCGTCGACGATAAGGTTTACGTCAAGGATGGCGTAACCGGTAAAGTGACGATTGGTGACGTGAGGAAAGTGGCGTCGCCGTTAGGGAATGTAACGGGAGCAGACTTGGATGATACGTGGTGGGACGCTGACGGGGTTGTTGTGACGGACGAGCGTTTTGACGAGTCAGCCATGCGTGGATGTGACTCGGCCGTCACGCGGTGCTGTAGCGCTGTTAGGTCTTATCTGTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

308

Amino Acids

33.37

Weight (kDa)

4.84

Isoelectric Point (pI)

39.47

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 617, 881
AatII GACGTC 1 cut(s) 282
AccB1I GGYRCC 1 cut(s) 173
AccI GTMKAC 2 cut(s) 554, 696
AccII CGCG 6 cut(s) 509, 591, 614, 616, 618, 889
AcoI YGGCCR 2 cut(s) 510, 879
AcyI GRCGYC 4 cut(s) 279, 686, 693, 764
AdeI CACNNNGTG 1 cut(s) 891
AfaI GTAC 1 cut(s) 359
AfeI AGCGCT 1 cut(s) 901
AfiI CCNNNNNNNGG 3 cut(s) 229, 665, 888
AgeI ACCGGT 1 cut(s) 728
AhdI GACNNNNNGTC 1 cut(s) 544
AjiI CACGTC 2 cut(s) 403, 751
AjuI GAANNNNNNNTTGG 2 cut(s) 434, 466
AluBI AGCT 6 cut(s) 119, 143, 317, 474, 532, 664
AluI AGCT 6 cut(s) 119, 143, 317, 474, 532, 664
Alw21I GWGCWC 2 cut(s) 18, 145
Alw26I GTCTC 3 cut(s) 115, 215, 552
AlwNI CAGNNNCTG 1 cut(s) 129
Ama87I CYCGRG 2 cut(s) 574, 665
Aor51HI AGCGCT 1 cut(s) 901
AoxI GGCC 5 cut(s) 231, 481, 510, 655, 879
ApaI GGGCCC 1 cut(s) 659
ApeKI GCWGC 3 cut(s) 238, 253, 661
ArsI GACNNNNNNTTYG 4 cut(s) 510, 542, 830, 862
AsiGI ACCGGT 1 cut(s) 728
AspLEI GCGC 5 cut(s) 258, 591, 616, 618, 902
AspS9I GGNCC 4 cut(s) 61, 232, 655, 656
AsuC2I CCSGG 1 cut(s) 96
AsuHPI GGTGA 1 cut(s) 758
AvaI CYCGRG 2 cut(s) 574, 665
AvaII GGWCC 1 cut(s) 61
BaeGI GKGCMC 1 cut(s) 659
BanI GGYRCC 1 cut(s) 173
BanII GRGCYC 2 cut(s) 145, 659
Bbv12I GWGCWC 2 cut(s) 18, 145
BbvI GCAGC 3 cut(s) 250, 265, 673
BccI CCATC 4 cut(s) 73, 212, 492, 713
BceAI ACGGC 4 cut(s) 280, 439, 754, 866
BcgI CGANNNNNNTGC 4 cut(s) 457, 491, 505, 539
BciVI GTATCC 1 cut(s) 337
BcnI CCSGG 1 cut(s) 96
BcoDI GTCTC 3 cut(s) 115, 215, 552
BfaI CTAG 1 cut(s) 599
BfmI CTRYAG 1 cut(s) 895
BfoI RGCGCY 2 cut(s) 259, 903
BfuI GTATCC 1 cut(s) 337
BglI GCCNNNNNGGC 1 cut(s) 264
BglII AGATCT 1 cut(s) 31
BisI GCNGC 6 cut(s) 217, 239, 242, 254, 510, 662
BlsI GCNGC 6 cut(s) 218, 240, 243, 255, 511, 663
Bme1390I CCNGG 1 cut(s) 96
Bme18I GGWCC 1 cut(s) 61
BmeRI GACNNNNNGTC 1 cut(s) 544
BmeT110I CYCGRG 2 cut(s) 574, 665
BmgBI CACGTC 2 cut(s) 403, 751
BmgT120I GGNCC 4 cut(s) 61, 232, 655, 656
BmiI GGNNCC 5 cut(s) 63, 175, 234, 656, 657
BmrFI CCNGG 1 cut(s) 96
BmrI ACTGGG 1 cut(s) 5
BmuI ACTGGG 1 cut(s) 5
BpuEI CTTGAG 1 cut(s) 99
BpuMI CCSGG 1 cut(s) 96
Bsa29I ATCGAT 2 cut(s) 89, 487
BsaAI YACGTR 1 cut(s) 807
BsaHI GRCGYC 4 cut(s) 279, 686, 693, 764
BsaWI WCCGGW 2 cut(s) 222, 728
BsaXI ACNNNNNCTCC 2 cut(s) 447, 477
Bsc4I CCNNNNNNNGG 3 cut(s) 229, 665, 888
Bse118I RCCGGY 2 cut(s) 512, 728
Bse1I ACTGG 1 cut(s) 11
BseCI ATCGAT 2 cut(s) 89, 487
BseGI GGATG 4 cut(s) 629, 724, 805, 875
BseLI CCNNNNNNNGG 3 cut(s) 229, 665, 888
BseNI ACTGG 1 cut(s) 11
BsePI GCGCGC 1 cut(s) 614
BseSI GKGCMC 1 cut(s) 659
BseX3I CGGCCG 2 cut(s) 510, 879
BseXI GCAGC 3 cut(s) 250, 265, 673
BseYI CCCAGC 1 cut(s) 249
Bsh1236I CGCG 6 cut(s) 509, 591, 614, 616, 618, 889
Bsh1285I CGRYCG 2 cut(s) 513, 882
BshFI GGCC 5 cut(s) 233, 483, 512, 657, 881
BshNI GGYRCC 1 cut(s) 173
BshTI ACCGGT 1 cut(s) 728
BshVI ATCGAT 2 cut(s) 89, 487
BsiEI CGRYCG 2 cut(s) 513, 882
BsiHKAI GWGCWC 2 cut(s) 18, 145
BsiHKCI CYCGRG 2 cut(s) 574, 665
BsiSI CCGG 6 cut(s) 59, 96, 223, 513, 635, 729
BsiWI CGTACG 1 cut(s) 357
BslFI GGGAC 4 cut(s) 468, 551, 623, 827
BslI CCNNNNNNNGG 3 cut(s) 229, 665, 888
BsmAI GTCTC 3 cut(s) 115, 215, 552
BsmBI CGTCTC 2 cut(s) 115, 552
BsmFI GGGAC 4 cut(s) 468, 551, 623, 827
BsnI GGCC 5 cut(s) 233, 483, 512, 657, 881
BsoBI CYCGRG 2 cut(s) 574, 665
Bsp120I GGGCCC 1 cut(s) 655
Bsp1286I GDGCHC 3 cut(s) 18, 145, 659
Bsp143I GATC 1 cut(s) 31
BspANI GGCC 5 cut(s) 233, 483, 512, 657, 881
BspDI ATCGAT 2 cut(s) 89, 487
BspFNI CGCG 6 cut(s) 509, 591, 614, 616, 618, 889
BspLI GGNNCC 5 cut(s) 63, 175, 234, 656, 657
BspT107I GGYRCC 1 cut(s) 173
BsrFI RCCGGY 2 cut(s) 512, 728
BsrI ACTGG 1 cut(s) 11
BssAI RCCGGY 2 cut(s) 512, 728
BssHII GCGCGC 1 cut(s) 614
BssMI GATC 1 cut(s) 31
BssNI GRCGYC 4 cut(s) 279, 686, 693, 764
Bst4CI ACNGT 1 cut(s) 125
BstACI GRCGYC 4 cut(s) 279, 686, 693, 764
BstAPI GCANNNNNTGC 1 cut(s) 161
BstBAI YACGTR 1 cut(s) 807
BstC8I GCNNGC 3 cut(s) 514, 616, 659
BstF5I GGATG 4 cut(s) 629, 724, 805, 875
BstFNI CGCG 6 cut(s) 509, 591, 614, 616, 618, 889
BstH2I RGCGCY 2 cut(s) 259, 903
BstHHI GCGC 5 cut(s) 258, 591, 616, 618, 902
BstKTI GATC 1 cut(s) 34
BstMAI GTCTC 3 cut(s) 115, 215, 552
BstMBI GATC 1 cut(s) 31
BstMCI CGRYCG 2 cut(s) 513, 882
BstMWI GCNNNNNNNGC 6 cut(s) 161, 250, 264, 480, 595, 644
BstSCI CCNGG 1 cut(s) 94
BstSFI CTRYAG 1 cut(s) 895
BstSLI GKGCMC 1 cut(s) 659
BstUI CGCG 6 cut(s) 509, 591, 614, 616, 618, 889
BstV1I GCAGC 3 cut(s) 250, 265, 673
BstX2I RGATCY 1 cut(s) 31
BstYI RGATCY 1 cut(s) 31
BstZI CGGCCG 2 cut(s) 510, 879
Bsu15I ATCGAT 2 cut(s) 89, 487
BsuI GTATCC 1 cut(s) 337
BsuRI GGCC 5 cut(s) 233, 483, 512, 657, 881
BsuTUI ATCGAT 2 cut(s) 89, 487
BtgZI GCGATG 1 cut(s) 300
BtrI CACGTC 2 cut(s) 403, 751
BtsCI GGATG 4 cut(s) 629, 724, 805, 875
BtsIMutI CAGTG 1 cut(s) 18
Cac8I GCNNGC 3 cut(s) 514, 616, 659
CaiI CAGNNNCTG 1 cut(s) 129
CfoI GCGC 5 cut(s) 258, 591, 616, 618, 902
Cfr10I RCCGGY 2 cut(s) 512, 728
Cfr13I GGNCC 4 cut(s) 61, 232, 655, 656
ClaI ATCGAT 2 cut(s) 89, 487
CseI GACGC 7 cut(s) 511, 607, 620, 675, 682, 753, 824
Csp6I GTAC 1 cut(s) 358
CspAI ACCGGT 1 cut(s) 728
CviAII CATG 2 cut(s) 495, 862
CviQI GTAC 1 cut(s) 358
DpnI GATC 1 cut(s) 33
DpnII GATC 1 cut(s) 31
DraIII CACNNNGTG 1 cut(s) 891
DrdI GACNNNNNNGTC 2 cut(s) 617, 881
DriI GACNNNNNGTC 1 cut(s) 544
DseDI GACNNNNNNGTC 2 cut(s) 617, 881
EaeI YGGCCR 2 cut(s) 510, 879
EagI CGGCCG 2 cut(s) 510, 879
Eam1105I GACNNNNNGTC 1 cut(s) 544
EciI GGCGGA 2 cut(s) 203, 449
Ecl136II GAGCTC 1 cut(s) 143
EclXI CGGCCG 2 cut(s) 510, 879
Eco24I GRGCYC 2 cut(s) 145, 659
Eco32I GATATC 1 cut(s) 298
Eco47I GGWCC 1 cut(s) 61
Eco47III AGCGCT 1 cut(s) 901
Eco52I CGGCCG 2 cut(s) 510, 879
Eco53kI GAGCTC 1 cut(s) 143
Eco88I CYCGRG 2 cut(s) 574, 665
EcoICRI GAGCTC 1 cut(s) 143
EcoO109I RGGNCCY 1 cut(s) 655
EcoRV GATATC 1 cut(s) 298
EcoT38I GRGCYC 2 cut(s) 145, 659
Esp3I CGTCTC 2 cut(s) 115, 552
FaeI CATG 2 cut(s) 498, 865
FaiI YATR 2 cut(s) 496, 863
FaqI GGGAC 4 cut(s) 468, 551, 623, 827
FatI CATG 2 cut(s) 494, 861
FauI CCCGC 3 cut(s) 50, 243, 666
FblI GTMKAC 2 cut(s) 554, 696
Fnu4HI GCNGC 6 cut(s) 217, 239, 242, 254, 510, 662
FokI GGATG 4 cut(s) 636, 731, 812, 882
FriOI GRGCYC 2 cut(s) 145, 659
Fsp4HI GCNGC 6 cut(s) 217, 239, 242, 254, 510, 662
FspBI CTAG 1 cut(s) 599
GlaI GCGC 5 cut(s) 257, 590, 615, 617, 901
GluI GCNGC 6 cut(s) 217, 239, 242, 254, 510, 662
GsaI CCCAGC 1 cut(s) 253
HaeII RGCGCY 2 cut(s) 259, 903
HaeIII GGCC 5 cut(s) 233, 483, 512, 657, 881
HapII CCGG 6 cut(s) 59, 96, 223, 513, 635, 729
HgaI GACGC 7 cut(s) 511, 607, 620, 675, 682, 753, 824
HhaI GCGC 5 cut(s) 258, 591, 616, 618, 902
Hin1I GRCGYC 4 cut(s) 279, 686, 693, 764
Hin1II CATG 2 cut(s) 498, 865
Hin6I GCGC 5 cut(s) 256, 589, 614, 616, 900
HinP1I GCGC 5 cut(s) 256, 589, 614, 616, 900
HincII GTYRAC 1 cut(s) 697
HindII GTYRAC 1 cut(s) 697
HindIII AAGCTT 2 cut(s) 117, 472
HpaII CCGG 6 cut(s) 59, 96, 223, 513, 635, 729
HphI GGTGA 1 cut(s) 758
Hpy166II GTNNAC 3 cut(s) 555, 697, 709
Hpy188I TCNGA 9 cut(s) 277, 331, 343, 349, 407, 479, 502, 623, 630
Hpy188III TCNNGA 2 cut(s) 130, 667
Hpy8I GTNNAC 3 cut(s) 555, 697, 709
HpyAV CCTTC 2 cut(s) 48, 178
HpyCH4III ACNGT 1 cut(s) 125
HpyCH4IV ACGT 7 cut(s) 108, 279, 402, 557, 711, 750, 806
HpyF10VI GCNNNNNNNGC 6 cut(s) 161, 250, 264, 480, 595, 644
HpySE526I ACGT 7 cut(s) 108, 279, 402, 557, 711, 750, 806
Hsp92I GRCGYC 4 cut(s) 279, 686, 693, 764
Hsp92II CATG 2 cut(s) 498, 865
HspAI GCGC 5 cut(s) 256, 589, 614, 616, 900
KroI GCCGGC 1 cut(s) 512
KroNI GCCGGC 1 cut(s) 514
Kzo9I GATC 1 cut(s) 31
LmnI GCTCC 2 cut(s) 140, 788
Lsp1109I GCAGC 3 cut(s) 250, 265, 673
MaeI CTAG 1 cut(s) 599
MaeII ACGT 7 cut(s) 108, 279, 402, 557, 711, 750, 806
MaeIII GTNAC 8 cut(s) 446, 724, 736, 746, 781, 832, 872, 883
MalI GATC 1 cut(s) 33
MauBI CGCGCGCG 1 cut(s) 614
MboI GATC 1 cut(s) 31
MboII GAAGA 1 cut(s) 26
MflI RGATCY 1 cut(s) 31
MhlI GDGCHC 3 cut(s) 18, 145, 659
MluCI AATT 2 cut(s) 24, 156
MlyI GAGTC 5 cut(s) 450, 533, 566, 863, 869
MmeI TCCRAC 4 cut(s) 300, 601, 608, 658
MnlI CCTC 9 cut(s) 61, 161, 188, 230, 331, 401, 473, 473, 747
MroNI GCCGGC 1 cut(s) 512
MspI CCGG 6 cut(s) 59, 96, 223, 513, 635, 729
MspR9I CCNGG 1 cut(s) 96
MvnI CGCG 6 cut(s) 509, 591, 614, 616, 618, 889
MwoI GCNNNNNNNGC 6 cut(s) 161, 250, 264, 480, 595, 644
NaeI GCCGGC 1 cut(s) 514
NciI CCSGG 1 cut(s) 96
NdeII GATC 1 cut(s) 31
NgoMIV GCCGGC 1 cut(s) 512
NlaIII CATG 2 cut(s) 498, 865
NlaIV GGNNCC 5 cut(s) 63, 175, 234, 656, 657
NmeAIII GCCGAG 1 cut(s) 857
NmuCI GTSAC 5 cut(s) 736, 746, 832, 872, 883
PauI GCGCGC 1 cut(s) 614
PcsI WCGNNNNNNNCGW 1 cut(s) 548
PdiI GCCGGC 1 cut(s) 514
PfeI GAWTC 7 cut(s) 91, 99, 338, 344, 442, 489, 631
Pfl23II CGTACG 1 cut(s) 357
PfoI TCCNGGA 1 cut(s) 94
PinAI ACCGGT 1 cut(s) 728
PkrI GCNGC 6 cut(s) 218, 240, 243, 255, 511, 663
PleI GAGTC 5 cut(s) 450, 533, 566, 862, 869
PpsI GAGTC 5 cut(s) 450, 533, 566, 862, 869
Ppu21I YACGTR 1 cut(s) 807
Psp124BI GAGCTC 1 cut(s) 145
PspFI CCCAGC 1 cut(s) 249
PspLI CGTACG 1 cut(s) 357
PspN4I GGNNCC 5 cut(s) 63, 175, 234, 656, 657
PspOMI GGGCCC 1 cut(s) 655
PspPI GGNCC 4 cut(s) 61, 232, 655, 656
PstNI CAGNNNCTG 1 cut(s) 129
PsuI RGATCY 1 cut(s) 31
PteI GCGCGC 1 cut(s) 614
RsaI GTAC 1 cut(s) 359
RsaNI GTAC 1 cut(s) 358
SacI GAGCTC 1 cut(s) 145
SalI GTCGAC 1 cut(s) 695
SatI GCNGC 6 cut(s) 217, 239, 242, 254, 510, 662
Sau3AI GATC 1 cut(s) 31
Sau96I GGNCC 4 cut(s) 61, 232, 655, 656
SchI GAGTC 5 cut(s) 450, 533, 566, 863, 869
ScrFI CCNGG 1 cut(s) 96
SduI GDGCHC 3 cut(s) 18, 145, 659
SfcI CTRYAG 1 cut(s) 895
SgrDI CGTCGACG 1 cut(s) 695
SinI GGWCC 1 cut(s) 61
SmlI CTYRAG 1 cut(s) 114
SmoI CTYRAG 1 cut(s) 114
Sse9I AATT 2 cut(s) 24, 156
SspMI CTAG 1 cut(s) 599
SstI GAGCTC 1 cut(s) 145
StyD4I CCNGG 1 cut(s) 94
TaaI ACNGT 1 cut(s) 125
TaiI ACGT 7 cut(s) 111, 282, 405, 560, 714, 753, 809
TaqI TCGA 3 cut(s) 89, 487, 696
TasI AATT 2 cut(s) 24, 156
TauI GCSGC 3 cut(s) 219, 244, 512
TfiI GAWTC 7 cut(s) 91, 99, 338, 344, 442, 489, 631
TscAI CASTG 1 cut(s) 18
TseFI GTSAC 5 cut(s) 736, 746, 832, 872, 883
TseI GCWGC 3 cut(s) 238, 253, 661
Tsp45I GTSAC 5 cut(s) 736, 746, 832, 872, 883
TspDTI ATGAA 1 cut(s) 405
TspGWI ACGGA 4 cut(s) 313, 447, 540, 851
TspRI CASTG 1 cut(s) 18
VpaK11BI GGWCC 1 cut(s) 61
XmiI GTMKAC 2 cut(s) 554, 696
XspI CTAG 1 cut(s) 599
ZraI GACGTC 1 cut(s) 280
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.