Rorug02G0108200

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000466
Physical Location & Seq
Forward (+)
9224685 .. 9229388
4704 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0108200.1

Sequence Viewer

Length: 2559 bp
ATGAAGCACCACCACCAGCACCATGCCGCCACCTGGAAACTCATCATACTCCTCCTCCTCCTCATCCTCGGCTCCACAGGCCCAAACGCCGCCGTTTCGGCCTCCGCGCTTCAGCGATCACAGCCGAACCACAACGCCGGCCTCTCTCGCTCTCCCAGCCGATCGCTCCTCTCCCATCCACCGCCTAAGCGTAGCACTCAATTTGTGGCGGTGCCGGAGGGGAAGTTACGGTTAGTGGACACTGATTCCGGTGCAGTAATTTATACTGTGGATTTAGGGGCGCCGCTGTACAGTTCATACCAGGCTCCTCAAGGCAAAGATAACAATAGGTTCCACGTAGATGTTGATGAAGATGGCGAGGTCTGTTTGGTCAGTAATGATATCAAACAGAACCTGCATATTCCAATATCAGAGTTTGTCCACAAGACGCCTTATACATTAGAGGATGGATCAGTTACACTTGGATCGAAGAAGACCACTACCTTTGTGATTGATCCGATGACCGGAAGGACAATTCACACATACACTTCATCTCGTGGAGAGGAGCAGGGTGTTCTGTACCCTGGTTTTATTAAAGGCATTAAGCAATGGATCAAGCCTGTTCCAACCAATCCAAATGCTGCTAAGGCGCTACTATATGTCACGGTGACAGAGTATTCCTTGAAGGCATACTTTCCACATTCAAATGAAGTCTCATGGAATTTGACATATTCTGAAATTGGAGTTATTTCATTTTGTTCAGATATTGAGAGTCCGGTTAGTGGGGCCTTTGGTTCTGAAGCAGGCAGTGATATTGTTTTACCGTTATCATGTCAATCAAAAATAAGAGTCCGCCATCAAAATCGTATATCGATTGAACCATCTAGACATGGCAGATTGTTAGAGGAACATAGCACAGATAAGATCCTCTCAGATCCCACTTCAAATGCAATGCATGTTGTGAGAAAATCTCCTAAAGGCATGTTAGTCATGCTTTTTGATTGGTCACGAGTTTTGACTCTCACTTTCTCCTTTGTCATCCTCATGGGCTTTGTTATATACTGCTATACTTATGTAGTTAAAAGGCAACAGCTCAGTAATTCTGATTCAAATACTGCACCTTCCAAGAGAAGGAAGAGTCGGAAATCAGAAAAGAATGCTAGTGGCATTGGTGAAGAAAAGGAGAAACATGTATCATCTCAGGATGAGGAAGCTTTAGCACACTCTGAGGGTGATAACAAAACATTGTTCCTTGATAAACTATTTTATGGTGCTGCAGTCGGTCGCAGGATTGGTAAACTACTTGTATCAAATACAGAGATCGCCAAGGGAAGCAATGGCACCATTGTCCTGGAGGGAGTTTATGAAGGTCGACCTGTTGCAGTCAAACGCCTTGTTCTAGCTCATCATGACGTGGCTTTTAAAGAAATTCAGAATCTTATTGCATCTGACCAACATCAGAACATAGTCCGATGGTACGGTGTGGAGTACGATCGAGATTTTGTCTATCTCTCTCTGGAGCGTTGTATTTGCAACTTGGATGATTTGATACAAATTTACTCAAATTCCGGTGAAAATCCTGTAGCTGGTGAGGAATATGCTATGATTCAAAATAAAGTTCGCTTGTCAACGGTAAAGAATCTTATGCCGAACGTTAATTTGTGGAAAGAAAATGGTCTATTGTCCCCCCTACTGTTGAAATTGATGAGGGATGTCGTCTGTGGGCTTGTGCATTTACATGAATTGGGGATAATTCATAGGGACTTAAAGCCTCAAAATGTTTTGTTAATTAAAGAAAGTTCATTTTGTGCAAAGCTTTCTGACATGGGCATCAGCAAGCGACTTATTGGGGACATGTCTTCTTTGGGTTCTGGTAGTTCGGGTTGGCAAGCACCTGAACAGCTTCTTCACGGGCGGCAAACACGTGCAGTGGATTTGTTTAGTTTAGGTTGTGTCCTCTTCTTTTGCATCACTGGTGGTAGACATCCATTTGGTGATCGCCTCGAACGTGATATAAATATTGTGAAGAACCAAATGGATCTCTTCTTGGTGGAGTATATTCCGGAAGCTTTGGATCTTATATCTCGATTGTTAAATCGTGACCCTGAATTGAGGCCAAAGGCTTTGGAGGTGTTGCACCATCCTCTTTTCTGGAGCTCTGAGTTGAGACTCTCATTCCTGCGAGACTATAGTGACAGGGTAGAACTGGAAGATAGACAGGCTAACTCTGATCTTCTGAAAGCCCTAGAAAGTACCGCGCCCGTGGCTTTGGGTGGGAAATGGAATGAAAAGATGGATCCTGCATTCCTCACTAGCATTGGCCATTACAGGCGTTATAAGTTTGACAGTGTTCGAGACTTACTGCGAGTCATGCGGAACAAATATAATCACTATAGAGAGCTCCCAAAGGAAATCCAGGAACTCCTAGGTCCAGTGCCCGAAGGATATGATCACTACTTTGCAAGTCGGTTCCCAAAACTTCTGATTGAAGTATACAAAGTTGCGTGCAAACATTGTAGAGAAGAGGAATGGTTTCAGAAGTATTTCAAAAGCACTGCCAATGAGTCCTCGGGACTCTAG

Protein Analysis

852

Amino Acids

96.01

Weight (kDa)

8.37

Isoelectric Point (pI)

45.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 431 - 704 9.2e-23 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 432 - 710 7.6e-34 Protein kinase domain
Ribonuc_2-5A PF06479 716 - 842 1.5e-46 Ribonuclease 2-5A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000466)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g35831 FvH4_3g44041 FvH4_5g15070 FvH4_5g15070 FvH4_7g08891
malus_domestica MD12G1125500.v1.1
prunus_persica Prupe.2G112700_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0322841 RchiOBHm_Chr1g0323391 RchiOBHm_Chr1g0327991 RchiOBHm_Chr1g0328961 RchiOBHm_Chr5g0080091 RchiOBHm_Chr6g0244841 RchiOBHm_Chr6g0250491
rosa_laevigata RLG00000024005 RLG00000024011 RLG00000024013 RLG00000024016 RLG00000024020 RLG00000024393 RLG00000030320 RLG00000030327 RLG00000030347 RLG00000030348 RLG00000030353 RLG00000030374 RLG00000034792 RLG00000035452 RLG00000036087 RLG00000036760 RLG00000036762 RLG00000036764 RLG00000036766 RLG00000036814 RLG00000036815 RLG00000036818 RLG00000036827 RLG00000036828 RLG00000036830 RLG00000036831 RLG00000036833 RLG00000036835
rosa_multiflora Rmu_co8082032.1_g000001 Rmu_co8162460.1_g000001 Rmu_co8167342.1_g000001 Rmu_co8180788.1_g000001 Rmu_co8300873.1_g000001 Rmu_sc0000252.1_g000008 Rmu_sc0000588.1_g000050 Rmu_sc0000595.1_g000020 Rmu_sc0000749.1_g000016 Rmu_sc0000749.1_g000039 Rmu_sc0000908.1_g000020 Rmu_sc0001148.1_g000040 Rmu_sc0001148.1_g000041 Rmu_sc0001226.1_g000014 Rmu_sc0001639.1_g000003 Rmu_sc0002075.1_g000005 Rmu_sc0002132.1_g000044 Rmu_sc0002634.1_g000026 Rmu_sc0003418.1_g000010 Rmu_sc0007793.1_g000030 Rmu_sc0012920.1_g000001 Rmu_sc0016164.1_g000016 Rmu_sc0019190.1_g000006 Rmu_sc0019291.1_g000001 Rmu_sc0021169.1_g000003 Rmu_sc0040474.1_g000001
rosa_roxburghii Rroxscaffold_1G00003030 Rroxscaffold_1G00003240 Rroxscaffold_1G00003280 Rroxscaffold_1G00003530 Rroxscaffold_1G00003600 Rroxscaffold_1G00003920 Rroxscaffold_3G00235100 Rroxscaffold_3G00240330 Rroxscaffold_4G00326470 Rroxscaffold_4G00326760 Rroxscaffold_4G00327070 Rroxscaffold_6G00393840 Rroxscaffold_6G00408110 Rroxscaffold_6G00408180
rosa_rugosa Rorug01G0036300 Rorug01G0038900 Rorug02G0108200 Rorug05G0459600 Rorug07G0125600 Rorug07G0204700
rosa_samantha Rh1DG060500 Rh6BG033900
rosa_wichuraiana Rw0G008150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2316
Acc36I ACCTGC 1 cut(s) 402
AccB1I GGYRCC 3 cut(s) 211, 280, 1319
AccI GTMKAC 3 cut(s) 1351, 1960, 2472
AccII CGCG 2 cut(s) 107, 2237
AccIII TCCGGA 1 cut(s) 2041
AclI AACGTT 1 cut(s) 1632
AcoI YGGCCR 1 cut(s) 2299
AcsI RAATTY 4 cut(s) 700, 1407, 1533, 1543
AcuI CTGAAG 2 cut(s) 95, 798
AcvI CACGTG 1 cut(s) 1904
AcyI GRCGYC 2 cut(s) 281, 428
AfaI GTAC 5 cut(s) 290, 560, 1457, 1469, 2233
AfiI CCNNNNNNNGG 5 cut(s) 33, 503, 761, 1110, 1565
AflIII ACRYGT 3 cut(s) 1168, 1833, 1901
AgsI TTSAA 9 cut(s) 664, 684, 857, 924, 1089, 1589, 1678, 2468, 2527
AjiI CACGTC 1 cut(s) 1393
AjnI CCWGG 5 cut(s) 32, 300, 562, 1329, 2394
AluBI AGCT 9 cut(s) 1072, 1193, 1382, 1565, 1795, 1882, 2048, 2136, 2380
AluI AGCT 9 cut(s) 1072, 1193, 1382, 1565, 1795, 1882, 2048, 2136, 2380
Alw21I GWGCWC 2 cut(s) 2138, 2382
Alw26I GTCTC 4 cut(s) 697, 2140, 2157, 2328
AlwNI CAGNNNCTG 1 cut(s) 394
Ama87I CYCGRG 1 cut(s) 2548
Aor13HI TCCGGA 1 cut(s) 2041
AoxI GGCC 6 cut(s) 79, 99, 139, 765, 2093, 2299
ApeKI GCWGC 2 cut(s) 620, 1253
ApoI RAATTY 4 cut(s) 700, 1407, 1533, 1543
Asp700I GAANNNNTTC 3 cut(s) 1881, 2511, 2522
AspA2I CCTAGG 1 cut(s) 2404
AspLEI GCGC 4 cut(s) 109, 283, 631, 2239
AspS9I GGNCC 3 cut(s) 80, 765, 2408
AsuHPI GGTGA 6 cut(s) 658, 1163, 1223, 1562, 1580, 1985
AvaI CYCGRG 1 cut(s) 2548
AvaII GGWCC 1 cut(s) 2408
AvrII CCTAGG 1 cut(s) 2404
BaeGI GKGCMC 1 cut(s) 2418
BalI TGGCCA 1 cut(s) 2301
BamHI GGATCC 1 cut(s) 2275
BanI GGYRCC 3 cut(s) 211, 280, 1319
BanII GRGCYC 2 cut(s) 2138, 2382
BauI CACGAG 2 cut(s) 534, 987
BbrPI CACGTG 1 cut(s) 1904
BbsI GAAGAC 2 cut(s) 479, 1830
Bbv12I GWGCWC 2 cut(s) 2138, 2382
BbvI GCAGC 2 cut(s) 607, 1240
BccI CCATC 8 cut(s) 183, 347, 440, 843, 868, 1446, 2127, 2266
BceAI ACGGC 1 cut(s) 77
BciT130I CCWGG 5 cut(s) 34, 302, 564, 1331, 2396
BclI TGATCA 1 cut(s) 2428
BcoDI GTCTC 4 cut(s) 697, 2140, 2157, 2328
BfaI CTAG 7 cut(s) 864, 1140, 1379, 2225, 2292, 2405, 2557
BfmI CTRYAG 4 cut(s) 1254, 1560, 2167, 2371
BfoI RGCGCY 2 cut(s) 284, 632
BfuAI ACCTGC 1 cut(s) 402
BglI GCCNNNNNGGC 1 cut(s) 98
BisI GCNGC 6 cut(s) 27, 90, 284, 621, 1254, 1895
BlnI CCTAGG 1 cut(s) 2404
BlsI GCNGC 6 cut(s) 28, 91, 285, 622, 1255, 1896
Bme1390I CCNGG 5 cut(s) 34, 302, 564, 1331, 2396
Bme18I GGWCC 1 cut(s) 2408
BmeT110I CYCGRG 1 cut(s) 2548
BmgBI CACGTC 1 cut(s) 1393
BmgT120I GGNCC 3 cut(s) 80, 765, 2408
BmiI GGNNCC 9 cut(s) 73, 213, 282, 306, 332, 766, 1321, 2277, 2450
BmrFI CCNGG 5 cut(s) 34, 302, 564, 1331, 2396
BmsI GCATC 3 cut(s) 1433, 1818, 1956
BpiI GAAGAC 2 cut(s) 479, 1830
BplI GAGNNNNNCTC 2 cut(s) 934, 966
BpmI CTGGAG 3 cut(s) 1352, 1517, 2152
Bpu10I CCTNAGC 2 cut(s) 186, 624
BpuEI CTTGAG 1 cut(s) 294
Bsa29I ATCGAT 1 cut(s) 851
BsaAI YACGTR 2 cut(s) 337, 1904
BsaHI GRCGYC 2 cut(s) 281, 428
BsaJI CCNNGG 6 cut(s) 67, 562, 1305, 2241, 2404, 2547
BsaWI WCCGGW 5 cut(s) 248, 503, 754, 1547, 2041
BsaXI ACNNNNNCTCC 4 cut(s) 39, 69, 209, 239
Bsc4I CCNNNNNNNGG 5 cut(s) 33, 503, 761, 1110, 1565
Bse118I RCCGGY 1 cut(s) 137
Bse1I ACTGG 3 cut(s) 1957, 2190, 2411
Bse3DI GCAATG 3 cut(s) 593, 936, 1321
BseAI TCCGGA 1 cut(s) 2041
BseBI CCWGG 5 cut(s) 34, 302, 564, 1331, 2396
BseCI ATCGAT 1 cut(s) 851
BseDI CCNNGG 6 cut(s) 67, 562, 1305, 2241, 2404, 2547
BseGI GGATG 9 cut(s) 63, 175, 451, 1017, 1189, 1525, 1696, 1963, 2119
BseLI CCNNNNNNNGG 5 cut(s) 33, 503, 761, 1110, 1565
BseMI GCAATG 3 cut(s) 593, 936, 1321
BseMII CTCAG 5 cut(s) 924, 1087, 1193, 1197, 2130
BseNI ACTGG 3 cut(s) 1957, 2190, 2411
BseRI GAGGAG 7 cut(s) 41, 44, 47, 50, 158, 297, 557
BseSI GKGCMC 1 cut(s) 2418
BseXI GCAGC 2 cut(s) 607, 1240
BseYI CCCAGC 1 cut(s) 155
BsgI GTGCAG 3 cut(s) 273, 1080, 1926
Bsh1236I CGCG 2 cut(s) 107, 2237
Bsh1285I CGRYCG 3 cut(s) 164, 1264, 1474
BshFI GGCC 6 cut(s) 81, 101, 141, 767, 2095, 2301
BshNI GGYRCC 3 cut(s) 211, 280, 1319
BshVI ATCGAT 1 cut(s) 851
BsiEI CGRYCG 3 cut(s) 164, 1264, 1474
BsiHKAI GWGCWC 2 cut(s) 2138, 2382
BsiHKCI CYCGRG 1 cut(s) 2548
BsiSI CCGG 7 cut(s) 138, 215, 249, 504, 755, 1548, 2042
BslFI GGGAC 3 cut(s) 1648, 1754, 1844
BslI CCNNNNNNNGG 5 cut(s) 33, 503, 761, 1110, 1565
BsmAI GTCTC 4 cut(s) 697, 2140, 2157, 2328
BsmFI GGGAC 3 cut(s) 1648, 1754, 1844
BsmI GAATGC 2 cut(s) 1141, 2282
BsnI GGCC 6 cut(s) 81, 101, 141, 767, 2095, 2301
BsoBI CYCGRG 1 cut(s) 2548
Bsp1286I GDGCHC 3 cut(s) 2138, 2382, 2418
Bsp13I TCCGGA 1 cut(s) 2041
Bsp1407I TGTACA 1 cut(s) 288
BspANI GGCC 6 cut(s) 81, 101, 141, 767, 2095, 2301
BspCNI CTCAG 5 cut(s) 923, 1086, 1192, 1198, 2131
BspDI ATCGAT 1 cut(s) 851
BspEI TCCGGA 1 cut(s) 2041
BspFNI CGCG 2 cut(s) 107, 2237
BspHI TCATGA 1 cut(s) 1387
BspLI GGNNCC 9 cut(s) 73, 213, 282, 306, 332, 766, 1321, 2277, 2450
BspMAI CTGCAG 1 cut(s) 1258
BspMI ACCTGC 1 cut(s) 402
BspT107I GGYRCC 3 cut(s) 211, 280, 1319
BsrDI GCAATG 3 cut(s) 593, 936, 1321
BsrFI RCCGGY 1 cut(s) 137
BsrGI TGTACA 1 cut(s) 288
BsrI ACTGG 3 cut(s) 1957, 2190, 2411
BssAI RCCGGY 1 cut(s) 137
BssECI CCNNGG 6 cut(s) 67, 562, 1305, 2241, 2404, 2547
BssNAI GTATAC 1 cut(s) 2473
BssNI GRCGYC 2 cut(s) 281, 428
BssSI CACGAG 2 cut(s) 534, 987
BssT1I CCWWGG 2 cut(s) 1305, 2404
Bst1107I GTATAC 1 cut(s) 2473
Bst2BI CACGAG 2 cut(s) 534, 987
Bst2UI CCWGG 5 cut(s) 34, 302, 564, 1331, 2396
Bst4CI ACNGT 9 cut(s) 231, 268, 293, 646, 804, 1460, 1612, 1674, 2327
Bst6I CTCTTC 4 cut(s) 1109, 1943, 2027, 2496
BstACI GRCGYC 2 cut(s) 281, 428
BstAUI TGTACA 1 cut(s) 288
BstBAI YACGTR 2 cut(s) 337, 1904
BstC8I GCNNGC 5 cut(s) 139, 784, 1817, 1869, 2485
BstDEI CTNAG 7 cut(s) 186, 624, 910, 1073, 1179, 1206, 2139
BstDSI CCRYGG 1 cut(s) 2241
BstF5I GGATG 9 cut(s) 63, 175, 451, 1017, 1189, 1525, 1696, 1963, 2119
BstFNI CGCG 2 cut(s) 107, 2237
BstH2I RGCGCY 2 cut(s) 284, 632
BstHHI GCGC 4 cut(s) 109, 283, 631, 2239
BstMAI GTCTC 4 cut(s) 697, 2140, 2157, 2328
BstMCI CGRYCG 3 cut(s) 164, 1264, 1474
BstMWI GCNNNNNNNGC 8 cut(s) 78, 98, 121, 147, 156, 626, 2243, 2350
BstNI CCWGG 5 cut(s) 34, 302, 564, 1331, 2396
BstNSI RCATGY 4 cut(s) 938, 964, 1172, 1837
BstSCI CCNGG 5 cut(s) 32, 300, 562, 1329, 2394
BstSFI CTRYAG 4 cut(s) 1254, 1560, 2167, 2371
BstSLI GKGCMC 1 cut(s) 2418
BstUI CGCG 2 cut(s) 107, 2237
BstV1I GCAGC 2 cut(s) 607, 1240
BstV2I GAAGAC 2 cut(s) 479, 1830
BstX2I RGATCY 5 cut(s) 903, 913, 2017, 2053, 2275
BstXI CCANNNNNNTGG 1 cut(s) 1330
BstYI RGATCY 5 cut(s) 903, 913, 2017, 2053, 2275
BstZ17I GTATAC 1 cut(s) 2473
Bsu15I ATCGAT 1 cut(s) 851
BsuRI GGCC 6 cut(s) 81, 101, 141, 767, 2095, 2301
BsuTUI ATCGAT 1 cut(s) 851
BtgI CCRYGG 1 cut(s) 2241
BtrI CACGTC 1 cut(s) 1393
BtsCI GGATG 9 cut(s) 63, 175, 451, 1017, 1189, 1525, 1696, 1963, 2119
BtsI GCAGTG 3 cut(s) 793, 1914, 2532
BtsIMutI CAGTG 7 cut(s) 240, 793, 1914, 1950, 2332, 2418, 2532
BveI ACCTGC 1 cut(s) 402
Cac8I GCNNGC 5 cut(s) 139, 784, 1817, 1869, 2485
CaiI CAGNNNCTG 1 cut(s) 394
CciI TCATGA 1 cut(s) 1387
CfoI GCGC 4 cut(s) 109, 283, 631, 2239
Cfr10I RCCGGY 1 cut(s) 137
Cfr13I GGNCC 3 cut(s) 80, 765, 2408
ClaI ATCGAT 1 cut(s) 851
CseI GACGC 1 cut(s) 436
Csp6I GTAC 5 cut(s) 289, 559, 1456, 1468, 2232
CspCI CAANNNNNGTGG 2 cut(s) 466, 501
CviQI GTAC 5 cut(s) 289, 559, 1456, 1468, 2232
DdeI CTNAG 7 cut(s) 186, 624, 910, 1073, 1179, 1206, 2139
DinI GGCGCC 1 cut(s) 282
DraI TTTAAA 1 cut(s) 1402
EaeI YGGCCR 1 cut(s) 2299
Eam1104I CTCTTC 4 cut(s) 1109, 1943, 2027, 2496
EarI CTCTTC 4 cut(s) 1109, 1943, 2027, 2496
EciI GGCGGA 1 cut(s) 821
Ecl136II GAGCTC 2 cut(s) 2136, 2380
Eco130I CCWWGG 2 cut(s) 1305, 2404
Eco24I GRGCYC 2 cut(s) 2138, 2382
Eco32I GATATC 1 cut(s) 382
Eco47I GGWCC 1 cut(s) 2408
Eco53kI GAGCTC 2 cut(s) 2136, 2380
Eco57I CTGAAG 2 cut(s) 95, 798
Eco72I CACGTG 1 cut(s) 1904
Eco88I CYCGRG 1 cut(s) 2548
EcoICRI GAGCTC 2 cut(s) 2136, 2380
EcoO109I RGGNCCY 1 cut(s) 765
EcoRII CCWGG 5 cut(s) 32, 300, 562, 1329, 2394
EcoRV GATATC 1 cut(s) 382
EcoT14I CCWWGG 2 cut(s) 1305, 2404
EcoT22I ATGCAT 1 cut(s) 936
EcoT38I GRGCYC 2 cut(s) 2138, 2382
EgeI GGCGCC 1 cut(s) 282
EheI GGCGCC 1 cut(s) 282
ErhI CCWWGG 2 cut(s) 1305, 2404
FalI AAGNNNNNCTT 2 cut(s) 656, 688
FaqI GGGAC 3 cut(s) 1648, 1754, 1844
FbaI TGATCA 1 cut(s) 2428
FblI GTMKAC 3 cut(s) 1351, 1960, 2472
Fnu4HI GCNGC 6 cut(s) 27, 90, 284, 621, 1254, 1895
FokI GGATG 9 cut(s) 50, 162, 458, 1004, 1196, 1532, 1703, 1950, 2106
FriOI GRGCYC 2 cut(s) 2138, 2382
Fsp4HI GCNGC 6 cut(s) 27, 90, 284, 621, 1254, 1895
FspBI CTAG 7 cut(s) 864, 1140, 1379, 2225, 2292, 2405, 2557
GlaI GCGC 4 cut(s) 108, 282, 630, 2238
GluI GCNGC 6 cut(s) 27, 90, 284, 621, 1254, 1895
GsaI CCCAGC 1 cut(s) 159
GsuI CTGGAG 3 cut(s) 1352, 1517, 2152
HaeII RGCGCY 2 cut(s) 284, 632
HaeIII GGCC 6 cut(s) 81, 101, 141, 767, 2095, 2301
HapII CCGG 7 cut(s) 138, 215, 249, 504, 755, 1548, 2042
HgaI GACGC 1 cut(s) 436
HhaI GCGC 4 cut(s) 109, 283, 631, 2239
Hin1I GRCGYC 2 cut(s) 281, 428
Hin6I GCGC 4 cut(s) 107, 281, 629, 2237
HinP1I GCGC 4 cut(s) 107, 281, 629, 2237
HincII GTYRAC 2 cut(s) 1352, 1608
HindII GTYRAC 2 cut(s) 1352, 1608
HindIII AAGCTT 3 cut(s) 1191, 1793, 2046
HpaII CCGG 7 cut(s) 138, 215, 249, 504, 755, 1548, 2042
HphI GGTGA 6 cut(s) 658, 1163, 1223, 1562, 1580, 1985
Hpy166II GTNNAC 7 cut(s) 238, 421, 1277, 1352, 1608, 1961, 2473
Hpy8I GTNNAC 7 cut(s) 238, 421, 1277, 1352, 1608, 1961, 2473
HpyAV CCTTC 6 cut(s) 501, 658, 1104, 1110, 1340, 2414
HpyCH4III ACNGT 9 cut(s) 231, 268, 293, 646, 804, 1460, 1612, 1674, 2327
HpyCH4IV ACGT 5 cut(s) 336, 1392, 1632, 1903, 1987
HpyF10VI GCNNNNNNNGC 8 cut(s) 78, 98, 121, 147, 156, 626, 2243, 2350
HpyF3I CTNAG 7 cut(s) 186, 624, 910, 1073, 1179, 1206, 2139
HpySE526I ACGT 5 cut(s) 336, 1392, 1632, 1903, 1987
Hsp92I GRCGYC 2 cut(s) 281, 428
HspAI GCGC 4 cut(s) 107, 281, 629, 2237
KasI GGCGCC 1 cut(s) 280
Kpn2I TCCGGA 1 cut(s) 2041
KroI GCCGGC 1 cut(s) 137
KroNI GCCGGC 1 cut(s) 139
Ksp22I TGATCA 1 cut(s) 2428
LmnI GCTCC 7 cut(s) 77, 171, 310, 544, 1498, 2133, 2385
Lsp1109I GCAGC 2 cut(s) 607, 1240
LweI GCATC 3 cut(s) 1433, 1818, 1956
MaeI CTAG 7 cut(s) 864, 1140, 1379, 2225, 2292, 2405, 2557
MaeII ACGT 5 cut(s) 336, 1392, 1632, 1903, 1987
MaeIII GTNAC 7 cut(s) 225, 454, 640, 646, 984, 2078, 2171
MflI RGATCY 5 cut(s) 903, 913, 2017, 2053, 2275
MhlI GDGCHC 3 cut(s) 2138, 2382, 2418
MlsI TGGCCA 1 cut(s) 2301
MluNI TGGCCA 1 cut(s) 2301
Mly113I GGCGCC 1 cut(s) 281
MlyI GAGTC 8 cut(s) 760, 837, 991, 1126, 2142, 2355, 2547, 2552
MmeI TCCRAC 2 cut(s) 629, 1100
Mox20I TGGCCA 1 cut(s) 2301
Mph1103I ATGCAT 1 cut(s) 936
MroI TCCGGA 1 cut(s) 2041
MroNI GCCGGC 1 cut(s) 137
MroXI GAANNNNTTC 3 cut(s) 1881, 2511, 2522
MscI TGGCCA 1 cut(s) 2301
MseI TTAA 9 cut(s) 573, 582, 1059, 1401, 1635, 1745, 1766, 1770, 2072
MslI CAYNNNNRTG 4 cut(s) 339, 684, 1022, 1716
Msp20I TGGCCA 1 cut(s) 2301
MspA1I CMGCKG 1 cut(s) 286
MspI CCGG 7 cut(s) 138, 215, 249, 504, 755, 1548, 2042
MspR9I CCNGG 5 cut(s) 34, 302, 564, 1331, 2396
Mva1269I GAATGC 2 cut(s) 1141, 2282
MvaI CCWGG 5 cut(s) 34, 302, 564, 1331, 2396
MvnI CGCG 2 cut(s) 107, 2237
MwoI GCNNNNNNNGC 8 cut(s) 78, 98, 121, 147, 156, 626, 2243, 2350
NaeI GCCGGC 1 cut(s) 139
NarI GGCGCC 1 cut(s) 281
NgoMIV GCCGGC 1 cut(s) 137
NlaIV GGNNCC 9 cut(s) 73, 213, 282, 306, 332, 766, 1321, 2277, 2450
NmeAIII GCCGAG 1 cut(s) 48
NmuCI GTSAC 5 cut(s) 640, 646, 984, 2078, 2171
NsiI ATGCAT 1 cut(s) 936
NspI RCATGY 4 cut(s) 938, 964, 1172, 1837
PacI TTAATTAA 1 cut(s) 1770
PagI TCATGA 1 cut(s) 1387
PciI ACATGT 2 cut(s) 1168, 1833
PcsI WCGNNNNNNNCGW 1 cut(s) 1984
PctI GAATGC 2 cut(s) 1141, 2282
PdiI GCCGGC 1 cut(s) 139
PdmI GAANNNNTTC 3 cut(s) 1881, 2511, 2522
PfeI GAWTC 5 cut(s) 245, 1085, 1414, 1585, 1618
PfoI TCCNGGA 2 cut(s) 1329, 2394
PkrI GCNGC 6 cut(s) 28, 91, 285, 622, 1255, 1896
Ple19I CGATCG 2 cut(s) 164, 1474
PleI GAGTC 8 cut(s) 759, 836, 991, 1125, 2142, 2354, 2547, 2551
PluTI GGCGCC 1 cut(s) 284
PmaCI CACGTG 1 cut(s) 1904
PmlI CACGTG 1 cut(s) 1904
PpsI GAGTC 8 cut(s) 759, 836, 991, 1125, 2142, 2354, 2547, 2551
Ppu21I YACGTR 2 cut(s) 337, 1904
PscI ACATGT 2 cut(s) 1168, 1833
PsiI TTATAA 1 cut(s) 2316
Psp124BI GAGCTC 2 cut(s) 2138, 2382
Psp1406I AACGTT 1 cut(s) 1632
Psp6I CCWGG 5 cut(s) 32, 300, 562, 1329, 2394
PspCI CACGTG 1 cut(s) 1904
PspFI CCCAGC 1 cut(s) 155
PspGI CCWGG 5 cut(s) 32, 300, 562, 1329, 2394
PspN4I GGNNCC 9 cut(s) 73, 213, 282, 306, 332, 766, 1321, 2277, 2450
PspPI GGNCC 3 cut(s) 80, 765, 2408
PstI CTGCAG 1 cut(s) 1258
PstNI CAGNNNCTG 1 cut(s) 394
PsuI RGATCY 5 cut(s) 903, 913, 2017, 2053, 2275
PvuI CGATCG 2 cut(s) 164, 1474
RsaI GTAC 5 cut(s) 290, 560, 1457, 1469, 2233
RsaNI GTAC 5 cut(s) 289, 559, 1456, 1468, 2232
RseI CAYNNNNRTG 4 cut(s) 339, 684, 1022, 1716
SacI GAGCTC 2 cut(s) 2138, 2382
SalI GTCGAC 1 cut(s) 1350
SaqAI TTAA 9 cut(s) 573, 582, 1059, 1401, 1635, 1745, 1766, 1770, 2072
SatI GCNGC 6 cut(s) 27, 90, 284, 621, 1254, 1895
Sau96I GGNCC 3 cut(s) 80, 765, 2408
SchI GAGTC 8 cut(s) 760, 837, 991, 1126, 2142, 2355, 2547, 2552
ScrFI CCNGG 5 cut(s) 34, 302, 564, 1331, 2396
SduI GDGCHC 3 cut(s) 2138, 2382, 2418
SfaNI GCATC 3 cut(s) 1433, 1818, 1956
SfcI CTRYAG 4 cut(s) 1254, 1560, 2167, 2371
SfoI GGCGCC 1 cut(s) 282
SinI GGWCC 1 cut(s) 2408
SmiMI CAYNNNNRTG 4 cut(s) 339, 684, 1022, 1716
SmlI CTYRAG 1 cut(s) 309
SmoI CTYRAG 1 cut(s) 309
SspDI GGCGCC 1 cut(s) 280
SspI AATATT 1 cut(s) 1999
SspMI CTAG 7 cut(s) 864, 1140, 1379, 2225, 2292, 2405, 2557
SstI GAGCTC 2 cut(s) 2138, 2382
StyD4I CCNGG 5 cut(s) 32, 300, 562, 1329, 2394
StyI CCWWGG 2 cut(s) 1305, 2404
TaaI ACNGT 9 cut(s) 231, 268, 293, 646, 804, 1460, 1612, 1674, 2327
TaiI ACGT 5 cut(s) 339, 1395, 1635, 1906, 1990
TaqI TCGA 7 cut(s) 467, 851, 1351, 1474, 1983, 2065, 2332
TaqII GACCGA 1 cut(s) 1250
TatI WGTACW 1 cut(s) 288
TauI GCSGC 4 cut(s) 29, 92, 286, 1897
TfiI GAWTC 5 cut(s) 245, 1085, 1414, 1585, 1618
Tru1I TTAA 9 cut(s) 573, 582, 1059, 1401, 1635, 1745, 1766, 1770, 2072
Tru9I TTAA 9 cut(s) 573, 582, 1059, 1401, 1635, 1745, 1766, 1770, 2072
TscAI CASTG 7 cut(s) 247, 793, 1914, 1957, 2332, 2418, 2539
TseFI GTSAC 5 cut(s) 640, 646, 984, 2078, 2171
TseI GCWGC 2 cut(s) 620, 1253
Tsp45I GTSAC 5 cut(s) 640, 646, 984, 2078, 2171
TspRI CASTG 7 cut(s) 247, 793, 1914, 1957, 2332, 2418, 2539
VpaK11BI GGWCC 1 cut(s) 2408
XapI RAATTY 4 cut(s) 700, 1407, 1533, 1543
XbaI TCTAGA 1 cut(s) 863
XceI RCATGY 4 cut(s) 938, 964, 1172, 1837
XmaJI CCTAGG 1 cut(s) 2404
XmiI GTMKAC 3 cut(s) 1351, 1960, 2472
XmnI GAANNNNTTC 3 cut(s) 1881, 2511, 2522
XspI CTAG 7 cut(s) 864, 1140, 1379, 2225, 2292, 2405, 2557
Zsp2I ATGCAT 1 cut(s) 936
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.