RLG00000036835

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000466
Physical Location & Seq
Reverse (-)
85514504 .. 85515235
732 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000036835

Sequence Viewer

Length: 732 bp
ATGTTGTTGGCGATCATTGTGGTAATTGTGACATGGATCGGTGCCATAGCTACTATTAAGTGGATATGGACTCTACTGAAGCATTGGTTCGGTTTCATGGTTGAAAGGCGTGAACCAATATCTCAGGATAATCAAGAATTGATTGACATCGCTACTGAGCCCGAGAGAGAAGAAGAAGAAAAGATGTGGGATCTCAGTGACTCTCCTGATCAGGGAAACGATGGCACCACCTCGGCAGCCGCACCGGTTGCTGCTTCTTCTTCTCTATCATCAGCTGTTGAGTGGAAGTATGATGTGTTTTTGAGTTTCAGGGGTCCTGACACTCGCAAGGGTATCACATTTGAACTATACGATCGACTGCAGAGGAGGGGAATTATTGCATTCATGGATGATCGAGATCTTGAAGTAGGGGATGTTATTTCTCCAACTCTCTTAAGAGCAATTAGAGAATCAAGGTTTGCAATCGTTGTTCTCTCGCAAAATTATGCTTCTTCTACTTGGTGTTTGGAGGAACTTAGAGAGATTTGTCTATGCATGGAAGACAACAGGATTTTGCCACTTTTTTATCATGTTAATCCTACTGATGTTCGATATCAGAAGAGGAGTTTCGAAGATGCTTTCTCTAAGCATGAAACCTCTGCGCGACATGAATCAGAGAAGGTGAAGCAATGGAAAGCTGCTTTAACCAAAGTGGCCAATATCTCTGGGTGGAATACAGATGATCATAAGTAA

Protein Analysis

244

Amino Acids

28.0

Weight (kDa)

5.08

Isoelectric Point (pI)

52.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 96 - 241 5e-45 TIR domain
TIR_2 PF13676 99 - 207 3e-16 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000466)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g35831 FvH4_3g44041 FvH4_5g15070 FvH4_5g15070 FvH4_7g08891
malus_domestica MD12G1125500.v1.1
prunus_persica Prupe.2G112700_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0322841 RchiOBHm_Chr1g0323391 RchiOBHm_Chr1g0327991 RchiOBHm_Chr1g0328961 RchiOBHm_Chr5g0080091 RchiOBHm_Chr6g0244841 RchiOBHm_Chr6g0250491
rosa_laevigata RLG00000024005 RLG00000024011 RLG00000024013 RLG00000024016 RLG00000024020 RLG00000024393 RLG00000030320 RLG00000030327 RLG00000030347 RLG00000030348 RLG00000030353 RLG00000030374 RLG00000034792 RLG00000035452 RLG00000036087 RLG00000036760 RLG00000036762 RLG00000036764 RLG00000036766 RLG00000036814 RLG00000036815 RLG00000036818 RLG00000036827 RLG00000036828 RLG00000036830 RLG00000036831 RLG00000036833 RLG00000036835
rosa_multiflora Rmu_co8082032.1_g000001 Rmu_co8162460.1_g000001 Rmu_co8167342.1_g000001 Rmu_co8180788.1_g000001 Rmu_co8300873.1_g000001 Rmu_sc0000252.1_g000008 Rmu_sc0000588.1_g000050 Rmu_sc0000595.1_g000020 Rmu_sc0000749.1_g000016 Rmu_sc0000749.1_g000039 Rmu_sc0000908.1_g000020 Rmu_sc0001148.1_g000040 Rmu_sc0001148.1_g000041 Rmu_sc0001226.1_g000014 Rmu_sc0001639.1_g000003 Rmu_sc0002075.1_g000005 Rmu_sc0002132.1_g000044 Rmu_sc0002634.1_g000026 Rmu_sc0003418.1_g000010 Rmu_sc0007793.1_g000030 Rmu_sc0012920.1_g000001 Rmu_sc0016164.1_g000016 Rmu_sc0019190.1_g000006 Rmu_sc0019291.1_g000001 Rmu_sc0021169.1_g000003 Rmu_sc0040474.1_g000001
rosa_roxburghii Rroxscaffold_1G00003030 Rroxscaffold_1G00003240 Rroxscaffold_1G00003280 Rroxscaffold_1G00003530 Rroxscaffold_1G00003600 Rroxscaffold_1G00003920 Rroxscaffold_3G00235100 Rroxscaffold_3G00240330 Rroxscaffold_4G00326470 Rroxscaffold_4G00326760 Rroxscaffold_4G00327070 Rroxscaffold_6G00393840 Rroxscaffold_6G00408110 Rroxscaffold_6G00408180
rosa_rugosa Rorug01G0036300 Rorug01G0038900 Rorug02G0108200 Rorug05G0459600 Rorug07G0125600 Rorug07G0204700
rosa_samantha Rh1DG060500 Rh6BG033900
rosa_wichuraiana Rw0G008150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 41, 224
AccII CGCG 1 cut(s) 643
AciI CCGC 1 cut(s) 240
AclWI GGATC 2 cut(s) 44, 198
AcoI YGGCCR 1 cut(s) 693
AcuI CTGAAG 1 cut(s) 98
AfiI CCNNNNNNNGG 1 cut(s) 212
AflII CTTAAG 1 cut(s) 433
AgeI ACCGGT 1 cut(s) 244
AgsI TTSAA 3 cut(s) 104, 344, 404
AluBI AGCT 3 cut(s) 50, 275, 677
AluI AGCT 3 cut(s) 50, 275, 677
AlwI GGATC 2 cut(s) 44, 198
Ama87I CYCGRG 1 cut(s) 161
AoxI GGCC 1 cut(s) 693
ApeKI GCWGC 3 cut(s) 236, 251, 677
AsiGI ACCGGT 1 cut(s) 244
AspLEI GCGC 1 cut(s) 643
AspS9I GGNCC 1 cut(s) 314
AsuHPI GGTGA 1 cut(s) 673
AsuII TTCGAA 1 cut(s) 609
AvaI CYCGRG 1 cut(s) 161
AvaII GGWCC 1 cut(s) 314
BalI TGGCCA 1 cut(s) 695
BanI GGYRCC 2 cut(s) 41, 224
BanII GRGCYC 1 cut(s) 162
BbsI GAAGAC 1 cut(s) 546
BbvI GCAGC 3 cut(s) 238, 248, 664
BccI CCATC 1 cut(s) 215
BclI TGATCA 2 cut(s) 208, 721
BfmI CTRYAG 1 cut(s) 359
BfrI CTTAAG 1 cut(s) 433
BglII AGATCT 1 cut(s) 397
BisI GCNGC 4 cut(s) 237, 240, 252, 678
BlsI GCNGC 4 cut(s) 238, 241, 253, 679
Bme18I GGWCC 1 cut(s) 314
BmeT110I CYCGRG 1 cut(s) 161
BmgT120I GGNCC 1 cut(s) 314
BmiI GGNNCC 3 cut(s) 43, 226, 315
BmsI GCATC 1 cut(s) 604
BpiI GAAGAC 1 cut(s) 546
Bpu14I TTCGAA 1 cut(s) 609
BsaBI GATNNNNATC 2 cut(s) 146, 396
BsaJI CCNNGG 1 cut(s) 231
BsaWI WCCGGW 1 cut(s) 244
Bsc4I CCNNNNNNNGG 1 cut(s) 212
Bse118I RCCGGY 1 cut(s) 244
Bse3DI GCAATG 1 cut(s) 674
Bse8I GATNNNNATC 2 cut(s) 146, 396
BseDI CCNNGG 1 cut(s) 231
BseGI GGATG 2 cut(s) 394, 418
BseJI GATNNNNATC 2 cut(s) 146, 396
BseLI CCNNNNNNNGG 1 cut(s) 212
BseMI GCAATG 1 cut(s) 674
BseMII CTCAG 3 cut(s) 137, 147, 208
BseRI GAGGAG 2 cut(s) 379, 616
BseXI GCAGC 3 cut(s) 238, 248, 664
Bsh1236I CGCG 1 cut(s) 643
Bsh1285I CGRYCG 1 cut(s) 355
BshFI GGCC 1 cut(s) 695
BshNI GGYRCC 2 cut(s) 41, 224
BshTI ACCGGT 1 cut(s) 244
BsiEI CGRYCG 1 cut(s) 355
BsiHKCI CYCGRG 1 cut(s) 161
BsiSI CCGG 1 cut(s) 245
BslI CCNNNNNNNGG 1 cut(s) 212
BsmI GAATGC 1 cut(s) 380
BsnI GGCC 1 cut(s) 695
BsoBI CYCGRG 1 cut(s) 161
Bsp119I TTCGAA 1 cut(s) 609
Bsp1286I GDGCHC 1 cut(s) 162
Bsp143I GATC 8 cut(s) 12, 36, 190, 208, 352, 391, 397, 721
BspACI CCGC 1 cut(s) 240
BspANI GGCC 1 cut(s) 695
BspCNI CTCAG 3 cut(s) 136, 148, 207
BspFNI CGCG 1 cut(s) 643
BspLI GGNNCC 3 cut(s) 43, 226, 315
BspMAI CTGCAG 1 cut(s) 363
BspPI GGATC 2 cut(s) 44, 198
BspT104I TTCGAA 1 cut(s) 609
BspT107I GGYRCC 2 cut(s) 41, 224
BspTI CTTAAG 1 cut(s) 433
BsrDI GCAATG 1 cut(s) 674
BsrFI RCCGGY 1 cut(s) 244
BssAI RCCGGY 1 cut(s) 244
BssECI CCNNGG 1 cut(s) 231
BssMI GATC 8 cut(s) 12, 36, 190, 208, 352, 391, 397, 721
Bst6I CTCTTC 1 cut(s) 593
BstAFI CTTAAG 1 cut(s) 433
BstAPI GCANNNNNTGC 1 cut(s) 248
BstBI TTCGAA 1 cut(s) 609
BstDEI CTNAG 5 cut(s) 123, 156, 194, 515, 624
BstF5I GGATG 2 cut(s) 394, 418
BstFNI CGCG 1 cut(s) 643
BstHHI GCGC 1 cut(s) 643
BstKTI GATC 8 cut(s) 15, 39, 193, 211, 355, 394, 400, 724
BstMBI GATC 8 cut(s) 12, 36, 190, 208, 352, 391, 397, 721
BstMCI CGRYCG 1 cut(s) 355
BstMWI GCNNNNNNNGC 1 cut(s) 248
BstSFI CTRYAG 1 cut(s) 359
BstUI CGCG 1 cut(s) 643
BstV1I GCAGC 3 cut(s) 238, 248, 664
BstV2I GAAGAC 1 cut(s) 546
BstX2I RGATCY 2 cut(s) 190, 397
BstYI RGATCY 2 cut(s) 190, 397
BsuRI GGCC 1 cut(s) 695
BtgZI GCGATG 1 cut(s) 133
BtsCI GGATG 2 cut(s) 394, 418
BtsIMutI CAGTG 1 cut(s) 202
CfoI GCGC 1 cut(s) 643
Cfr10I RCCGGY 1 cut(s) 244
Cfr13I GGNCC 1 cut(s) 314
CspAI ACCGGT 1 cut(s) 244
CviAII CATG 7 cut(s) 33, 97, 385, 535, 569, 629, 647
CviJI RGCY 6 cut(s) 50, 160, 239, 275, 677, 695
CviKI_1 RGCY 6 cut(s) 50, 160, 239, 275, 677, 695
DdeI CTNAG 5 cut(s) 123, 156, 194, 515, 624
DpnI GATC 8 cut(s) 14, 38, 192, 210, 354, 393, 399, 723
DpnII GATC 8 cut(s) 12, 36, 190, 208, 352, 391, 397, 721
EaeI YGGCCR 1 cut(s) 693
Eam1104I CTCTTC 1 cut(s) 593
EarI CTCTTC 1 cut(s) 593
Eco24I GRGCYC 1 cut(s) 162
Eco32I GATATC 1 cut(s) 593
Eco47I GGWCC 1 cut(s) 314
Eco57I CTGAAG 1 cut(s) 98
Eco88I CYCGRG 1 cut(s) 161
EcoO109I RGGNCCY 1 cut(s) 314
EcoRV GATATC 1 cut(s) 593
EcoT22I ATGCAT 1 cut(s) 536
EcoT38I GRGCYC 1 cut(s) 162
FaeI CATG 7 cut(s) 36, 100, 388, 538, 572, 632, 650
FatI CATG 7 cut(s) 32, 96, 384, 534, 568, 628, 646
FbaI TGATCA 2 cut(s) 208, 721
Fnu4HI GCNGC 4 cut(s) 237, 240, 252, 678
FokI GGATG 2 cut(s) 401, 425
FriOI GRGCYC 1 cut(s) 162
Fsp4HI GCNGC 4 cut(s) 237, 240, 252, 678
GlaI GCGC 1 cut(s) 642
GluI GCNGC 4 cut(s) 237, 240, 252, 678
HaeIII GGCC 1 cut(s) 695
HapII CCGG 1 cut(s) 245
HhaI GCGC 1 cut(s) 643
Hin1II CATG 7 cut(s) 36, 100, 388, 538, 572, 632, 650
Hin6I GCGC 1 cut(s) 641
HinP1I GCGC 1 cut(s) 641
HinfI GANTC 4 cut(s) 70, 200, 449, 650
HpaII CCGG 1 cut(s) 245
HphI GGTGA 1 cut(s) 673
Hpy166II GTNNAC 1 cut(s) 113
Hpy188I TCNGA 2 cut(s) 597, 655
Hpy188III TCNNGA 6 cut(s) 125, 134, 206, 317, 395, 401
Hpy8I GTNNAC 1 cut(s) 113
HpyAV CCTTC 1 cut(s) 652
HpyCH4V TGCA 4 cut(s) 361, 380, 461, 534
HpyF10VI GCNNNNNNNGC 1 cut(s) 248
HpyF3I CTNAG 5 cut(s) 123, 156, 194, 515, 624
Hsp92II CATG 7 cut(s) 36, 100, 388, 538, 572, 632, 650
HspAI GCGC 1 cut(s) 641
Ksp22I TGATCA 2 cut(s) 208, 721
Kzo9I GATC 8 cut(s) 12, 36, 190, 208, 352, 391, 397, 721
LpnPI CCDG 8 cut(s) 110, 197, 219, 258, 295, 330, 532, 690
Lsp1109I GCAGC 3 cut(s) 238, 248, 664
LweI GCATC 1 cut(s) 604
MaeIII GTNAC 2 cut(s) 28, 197
MalI GATC 8 cut(s) 14, 38, 192, 210, 354, 393, 399, 723
MboI GATC 8 cut(s) 12, 36, 190, 208, 352, 391, 397, 721
MboII GAAGA 9 cut(s) 182, 185, 188, 249, 252, 483, 551, 610, 623
MflI RGATCY 2 cut(s) 190, 397
MhlI GDGCHC 1 cut(s) 162
MlsI TGGCCA 1 cut(s) 695
MluCI AATT 5 cut(s) 24, 137, 372, 441, 481
MluNI TGGCCA 1 cut(s) 695
MlyI GAGTC 2 cut(s) 64, 194
MmeI TCCRAC 1 cut(s) 449
MnlI CCTC 6 cut(s) 241, 357, 360, 502, 594, 646
Mox20I TGGCCA 1 cut(s) 695
Mph1103I ATGCAT 1 cut(s) 536
MscI TGGCCA 1 cut(s) 695
MseI TTAA 4 cut(s) 57, 434, 573, 683
Msp20I TGGCCA 1 cut(s) 695
MspA1I CMGCKG 1 cut(s) 275
MspCI CTTAAG 1 cut(s) 433
MspI CCGG 1 cut(s) 245
Mva1269I GAATGC 1 cut(s) 380
MvnI CGCG 1 cut(s) 643
MwoI GCNNNNNNNGC 1 cut(s) 248
NdeII GATC 8 cut(s) 12, 36, 190, 208, 352, 391, 397, 721
NlaIII CATG 7 cut(s) 36, 100, 388, 538, 572, 632, 650
NlaIV GGNNCC 3 cut(s) 43, 226, 315
NmeAIII GCCGAG 1 cut(s) 212
NmuCI GTSAC 2 cut(s) 28, 197
NsiI ATGCAT 1 cut(s) 536
NspV TTCGAA 1 cut(s) 609
PctI GAATGC 1 cut(s) 380
PfeI GAWTC 2 cut(s) 449, 650
PinAI ACCGGT 1 cut(s) 244
PkrI GCNGC 4 cut(s) 238, 241, 253, 679
Ple19I CGATCG 1 cut(s) 355
PleI GAGTC 2 cut(s) 64, 194
PpsI GAGTC 2 cut(s) 64, 194
PpuMI RGGWCCY 1 cut(s) 314
Psp5II RGGWCCY 1 cut(s) 314
PspN4I GGNNCC 3 cut(s) 43, 226, 315
PspPI GGNCC 1 cut(s) 314
PspPPI RGGWCCY 1 cut(s) 314
PstI CTGCAG 1 cut(s) 363
PsuI RGATCY 2 cut(s) 190, 397
PvuI CGATCG 1 cut(s) 355
PvuII CAGCTG 1 cut(s) 275
SaqAI TTAA 4 cut(s) 57, 434, 573, 683
SatI GCNGC 4 cut(s) 237, 240, 252, 678
Sau3AI GATC 8 cut(s) 12, 36, 190, 208, 352, 391, 397, 721
Sau96I GGNCC 1 cut(s) 314
SchI GAGTC 2 cut(s) 64, 194
SduI GDGCHC 1 cut(s) 162
SetI ASST 7 cut(s) 52, 233, 277, 458, 638, 663, 679
SfaNI GCATC 1 cut(s) 604
SfcI CTRYAG 1 cut(s) 359
SfuI TTCGAA 1 cut(s) 609
SinI GGWCC 1 cut(s) 314
SmlI CTYRAG 1 cut(s) 433
SmoI CTYRAG 1 cut(s) 433
Sse9I AATT 5 cut(s) 24, 137, 372, 441, 481
SsiI CCGC 1 cut(s) 240
TaqI TCGA 4 cut(s) 355, 394, 589, 609
TasI AATT 5 cut(s) 24, 137, 372, 441, 481
TauI GCSGC 1 cut(s) 242
TfiI GAWTC 2 cut(s) 449, 650
Tru1I TTAA 4 cut(s) 57, 434, 573, 683
Tru9I TTAA 4 cut(s) 57, 434, 573, 683
TscAI CASTG 1 cut(s) 202
TseFI GTSAC 2 cut(s) 28, 197
TseI GCWGC 3 cut(s) 236, 251, 677
Tsp45I GTSAC 2 cut(s) 28, 197
TspDTI ATGAA 4 cut(s) 85, 373, 645, 663
TspRI CASTG 1 cut(s) 202
Vha464I CTTAAG 1 cut(s) 433
VpaK11BI GGWCC 1 cut(s) 314
Zsp2I ATGCAT 1 cut(s) 536
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.