Rorug07G0204700

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0000466
Physical Location & Seq
Reverse (-)
17964755 .. 17966740
1986 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0204700.1

Sequence Viewer

Length: 1986 bp
ATGGCGCACGGTGATGATGAGAGACCGAGTCAAAAGCCCAAATTCTCTAATAACGACGATGGCAAAGCAACAGACTATAGCAAATTAGAATGGAAGCCTGACCACCCCAATCGGCCTCTGTGGATTTGTCCCGATGGTCGAGTACTCCTGGAAACTAACTCGGCTTTTTACGAACACGCTCAGGATTTTCTCATTGCCATTGCCGAACCAGTTCGCAGGGCAGAGTTGATCCACGAGTATAACTTGACCCCGGAGTCATTGTGTGCGGCTGCCTCAACTGGTGTCAAAACAGAAACCATTATTTCTGTCTTGAATAAGCTCTCGAAAACCGAGCTCACAAAACAACTCAATGATTTTATTCAGTCTTCTACTGCTAATAGCGGCAAAGCGAAGCTGGTCCTCAAGAACAATCGGTACTTTGTGGAATCTACATTCCCGGAGGTACTGAACACATTGCTTGAGGATAAACTTATATCTGAAGCAAAAATTAGTAGTATAACAAATAATGGCAAAGAATTCGAAATTGATCCTAACAAGGTTGAAGACATCATGAAACGTTGCTCGCAAGATGGTTTGAACTATCCCATGGTGGAGGAGTACGACTATAGAAACGATAATGTAAACCCCAATGTTGGTATGGAACTAAAGCCCCAAGCACGCGCGACGATACGACCATATCAGCTTAAGAGCTTGAGCACAATGTTTGGTAATGGTAGAGCGAGATCTGGTATAATTGTACTACCTTGCGGCGCAGGAAAATCCCTAGTTGGTGTTTCTACAGCCTCCACAATAGGAAAGAGCTGCCTTTGTTTAGCAACGAATGGTGTTTCAGTTGATCAGTGGGTCTATCAGTTCAAGTTGTGGTCAACCATTCAAGATGAACAGATTTGTCGTTTCAAATCTGACTGTAAAGAAGAAGCTGCAGAGATGTTAATCCGCCGCAAAAGCAATGCTGCTGGGGTGGTGGTTGTGACTACATATAGCATGCTTGGCTGTGGTGGCAATAGGTCCCAACAGTCTAAAAACATTATTGAGGAAATAAAAAATAGAGAATGGGGGTTGATTCTCATGGATGAGGTTCATGTAACTCCTGCTGAATCGTTTCGAAATGTCATCCGAATCACCAAATCACACTGCAAGCTTGGCCTCACGGCGACGCTCTTGAGAGAAGATGACAAGATATCAGATCTCAACTTCCTCATTGGACCCAAATTATATGAAGCAAACTGGTTGGATTTGGCGAAACGAGGATACATTGCAAATGTGCTGTGCGCTGAAGTAAGGTGTCAGATGACAAAGGAGTTCTTTGCCAAGTATAAGAAAGAAAACTCCTACGAAAGAAAGCAAGCACTTTATTTGATGAATCCAAACAAGTTTAGGATATGCGAGTATCTTGTACAGTTTCATGAGCAGCAGCGCCGTGGTGATAAGATCATAGTCTTTTCCGACAACCTCTTCACCCTCACTGAATATGCCACCAAGCTCCGAAAACCTATGATTTATGGTGCCACCACGCAGGCGGAGACGACAAAAGTTCTACAAGAGTTCAAAACAAACCCGGACGTAAACACCGTATTCATGTCTAAGGTGGGTGATAACTCCTTGGACATTCCCGAGGCAAATGTGATCATTGAGATTTCATGGCACGGCGGTTCGAGACGACAAGAAGCGCAGCGTCTTGGTCGGATTCTAAGGGCCAAGAAAAGTAAAGAGAAGTACAATGCATTCTTCTATTCCCTTGTGTCATCGGATACACGGGAGATGAAATTTGCAGCTAAAAGGCAGCAGTTTTTGGTCGATCAAGGTTATAGTTATAAGATTGTTACAACCTTGCAATTGCCTAATTTTCCTCCACTTGGATCAGGAGCTTTGAGCTATGACAGTCTTCAAGATCAACGCGCACTACTCAAGAAAGTGTTGAGCTTTAATGAGGCTATAGTTGGGGCCGAAGATCCACTAGTAGAAGATGTAGATGGCATGCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

661

Amino Acids

74.82

Weight (kDa)

8.35

Isoelectric Point (pI)

33.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Helicase_C_3 PF13625 40 - 165 4.5e-30 Helicase conserved C-terminal domain
ResIII PF04851 222 - 387 2.1e-16 Type III restriction enzyme, res subunit
ERCC3_RAD25_C PF16203 413 - 648 1.3e-103 ERCC3/RAD25/XPB C-terminal helicase
Helicase_C PF00271 463 - 566 1.2e-12 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000466)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g35831 FvH4_3g44041 FvH4_5g15070 FvH4_5g15070 FvH4_7g08891
malus_domestica MD12G1125500.v1.1
prunus_persica Prupe.2G112700_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0322841 RchiOBHm_Chr1g0323391 RchiOBHm_Chr1g0327991 RchiOBHm_Chr1g0328961 RchiOBHm_Chr5g0080091 RchiOBHm_Chr6g0244841 RchiOBHm_Chr6g0250491
rosa_laevigata RLG00000024005 RLG00000024011 RLG00000024013 RLG00000024016 RLG00000024020 RLG00000024393 RLG00000030320 RLG00000030327 RLG00000030347 RLG00000030348 RLG00000030353 RLG00000030374 RLG00000034792 RLG00000035452 RLG00000036087 RLG00000036760 RLG00000036762 RLG00000036764 RLG00000036766 RLG00000036814 RLG00000036815 RLG00000036818 RLG00000036827 RLG00000036828 RLG00000036830 RLG00000036831 RLG00000036833 RLG00000036835
rosa_multiflora Rmu_co8082032.1_g000001 Rmu_co8162460.1_g000001 Rmu_co8167342.1_g000001 Rmu_co8180788.1_g000001 Rmu_co8300873.1_g000001 Rmu_sc0000252.1_g000008 Rmu_sc0000588.1_g000050 Rmu_sc0000595.1_g000020 Rmu_sc0000749.1_g000016 Rmu_sc0000749.1_g000039 Rmu_sc0000908.1_g000020 Rmu_sc0001148.1_g000040 Rmu_sc0001148.1_g000041 Rmu_sc0001226.1_g000014 Rmu_sc0001639.1_g000003 Rmu_sc0002075.1_g000005 Rmu_sc0002132.1_g000044 Rmu_sc0002634.1_g000026 Rmu_sc0003418.1_g000010 Rmu_sc0007793.1_g000030 Rmu_sc0012920.1_g000001 Rmu_sc0016164.1_g000016 Rmu_sc0019190.1_g000006 Rmu_sc0019291.1_g000001 Rmu_sc0021169.1_g000003 Rmu_sc0040474.1_g000001
rosa_roxburghii Rroxscaffold_1G00003030 Rroxscaffold_1G00003240 Rroxscaffold_1G00003280 Rroxscaffold_1G00003530 Rroxscaffold_1G00003600 Rroxscaffold_1G00003920 Rroxscaffold_3G00235100 Rroxscaffold_3G00240330 Rroxscaffold_4G00326470 Rroxscaffold_4G00326760 Rroxscaffold_4G00327070 Rroxscaffold_6G00393840 Rroxscaffold_6G00408110 Rroxscaffold_6G00408180
rosa_rugosa Rorug01G0036300 Rorug01G0038900 Rorug02G0108200 Rorug05G0459600 Rorug07G0125600 Rorug07G0204700
rosa_samantha Rh1DG060500 Rh6BG033900
rosa_wichuraiana Rw0G008150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1815
AasI GACNNNNNNGTC 1 cut(s) 253
AccB1I GGYRCC 1 cut(s) 1505
AccII CGCG 3 cut(s) 660, 662, 1899
AciI CCGC 7 cut(s) 266, 381, 747, 937, 940, 1520, 1650
AclI AACGTT 1 cut(s) 556
AclWI GGATC 4 cut(s) 223, 521, 1867, 1946
AcsI RAATTY 3 cut(s) 41, 515, 1766
AcuI CTGAAG 2 cut(s) 498, 1296
AfaI GTAC 7 cut(s) 144, 416, 444, 599, 738, 1398, 1718
AfiI CCNNNNNNNGG 2 cut(s) 632, 1856
AflII CTTAAG 1 cut(s) 683
AgsI TTSAA 8 cut(s) 313, 542, 577, 856, 875, 898, 1549, 1889
AhlI ACTAGT 1 cut(s) 1957
AjnI CCWGG 1 cut(s) 147
Alw21I GWGCWC 2 cut(s) 336, 698
Alw26I GTCTC 3 cut(s) 16, 1517, 1651
AlwI GGATC 4 cut(s) 223, 521, 1867, 1946
Ama87I CYCGRG 1 cut(s) 1613
AoxI GGCC 4 cut(s) 113, 1144, 1695, 1944
ApeKI GCWGC 9 cut(s) 269, 801, 920, 953, 1411, 1414, 1672, 1772, 1783
ApoI RAATTY 3 cut(s) 41, 515, 1766
Asp700I GAANNNNTTC 2 cut(s) 210, 1101
AspLEI GCGC 7 cut(s) 7, 662, 752, 1274, 1419, 1672, 1901
AspS9I GGNCC 5 cut(s) 397, 1008, 1205, 1695, 1944
AsuC2I CCSGG 3 cut(s) 251, 437, 1559
AsuHPI GGTGA 5 cut(s) 23, 1114, 1436, 1450, 1604
AsuII TTCGAA 2 cut(s) 519, 1105
AvaI CYCGRG 1 cut(s) 1613
AvaII GGWCC 3 cut(s) 397, 1008, 1205
BanI GGYRCC 1 cut(s) 1505
BanII GRGCYC 1 cut(s) 336
BauI CACGAG 1 cut(s) 233
BbsI GAAGAC 3 cut(s) 357, 549, 1877
Bbv12I GWGCWC 2 cut(s) 336, 698
BbvI GCAGC 9 cut(s) 256, 788, 907, 940, 1423, 1426, 1684, 1784, 1795
BccI CCATC 4 cut(s) 53, 128, 563, 1967
BceAI ACGGC 3 cut(s) 1167, 1404, 1663
BcgI CGANNNNNNTGC 2 cut(s) 499, 533
BciT130I CCWGG 1 cut(s) 149
BciVI GTATCC 2 cut(s) 1244, 1744
BclI TGATCA 2 cut(s) 835, 1626
BcnI CCSGG 3 cut(s) 251, 437, 1559
BcoDI GTCTC 3 cut(s) 16, 1517, 1651
BcuI ACTAGT 1 cut(s) 1957
BfaI CTAG 2 cut(s) 764, 1958
BfmI CTRYAG 5 cut(s) 76, 604, 777, 921, 1935
BfoI RGCGCY 1 cut(s) 1420
BfrI CTTAAG 1 cut(s) 683
BfuI GTATCC 2 cut(s) 1244, 1744
BglII AGATCT 2 cut(s) 722, 1186
BmcAI AGTACT 1 cut(s) 144
Bme1390I CCNGG 4 cut(s) 149, 251, 437, 1559
Bme18I GGWCC 3 cut(s) 397, 1008, 1205
BmeT110I CYCGRG 1 cut(s) 1613
BmgT120I GGNCC 5 cut(s) 397, 1008, 1205, 1695, 1944
BmiI GGNNCC 4 cut(s) 1010, 1207, 1507, 1945
BmrFI CCNGG 4 cut(s) 149, 251, 437, 1559
BpiI GAAGAC 3 cut(s) 357, 549, 1877
Bpu10I CCTNAGC 1 cut(s) 180
Bpu14I TTCGAA 2 cut(s) 519, 1105
BpuEI CTTGAG 5 cut(s) 386, 479, 712, 1183, 1892
BpuMI CCSGG 3 cut(s) 251, 437, 1559
BsaBI GATNNNNATC 1 cut(s) 932
BsaI GGTCTC 1 cut(s) 16
BsaJI CCNNGG 5 cut(s) 249, 585, 1420, 1602, 1614
BsaXI ACNNNNNCTCC 2 cut(s) 245, 275
Bsc4I CCNNNNNNNGG 2 cut(s) 632, 1856
Bse1I ACTGG 3 cut(s) 209, 283, 1232
Bse3DI GCAATG 5 cut(s) 192, 198, 452, 955, 1254
Bse8I GATNNNNATC 1 cut(s) 932
BseBI CCWGG 1 cut(s) 149
BseDI CCNNGG 5 cut(s) 249, 585, 1420, 1602, 1614
BseGI GGATG 2 cut(s) 1078, 1113
BseJI GATNNNNATC 1 cut(s) 932
BseLI CCNNNNNNNGG 2 cut(s) 632, 1856
BseMI GCAATG 5 cut(s) 192, 198, 452, 955, 1254
BseMII CTCAG 1 cut(s) 194
BseNI ACTGG 3 cut(s) 209, 283, 1232
BseRI GAGGAG 1 cut(s) 608
BseXI GCAGC 9 cut(s) 256, 788, 907, 940, 1423, 1426, 1684, 1784, 1795
BseYI CCCAGC 1 cut(s) 956
Bsh1236I CGCG 3 cut(s) 660, 662, 1899
BshFI GGCC 4 cut(s) 115, 1146, 1697, 1946
BshNI GGYRCC 1 cut(s) 1505
BsiHKAI GWGCWC 2 cut(s) 336, 698
BsiHKCI CYCGRG 1 cut(s) 1613
BsiSI CCGG 3 cut(s) 251, 437, 1559
BslFI GGGAC 2 cut(s) 114, 994
BslI CCNNNNNNNGG 2 cut(s) 632, 1856
BsmAI GTCTC 3 cut(s) 16, 1517, 1651
BsmBI CGTCTC 2 cut(s) 1517, 1651
BsmFI GGGAC 2 cut(s) 114, 994
BsmI GAATGC 1 cut(s) 1724
BsnI GGCC 4 cut(s) 115, 1146, 1697, 1946
Bso31I GGTCTC 1 cut(s) 16
BsoBI CYCGRG 1 cut(s) 1613
Bsp119I TTCGAA 2 cut(s) 519, 1105
Bsp1286I GDGCHC 2 cut(s) 336, 698
Bsp1407I TGTACA 1 cut(s) 1396
Bsp19I CCATGG 1 cut(s) 585
BspACI CCGC 7 cut(s) 266, 381, 747, 937, 940, 1520, 1650
BspANI GGCC 4 cut(s) 115, 1146, 1697, 1946
BspCNI CTCAG 1 cut(s) 193
BspFNI CGCG 3 cut(s) 660, 662, 1899
BspHI TCATGA 2 cut(s) 549, 1405
BspLI GGNNCC 4 cut(s) 1010, 1207, 1507, 1945
BspMAI CTGCAG 1 cut(s) 925
BspPI GGATC 4 cut(s) 223, 521, 1867, 1946
BspT104I TTCGAA 2 cut(s) 519, 1105
BspT107I GGYRCC 1 cut(s) 1505
BspTI CTTAAG 1 cut(s) 683
BspTNI GGTCTC 1 cut(s) 16
BsrDI GCAATG 5 cut(s) 192, 198, 452, 955, 1254
BsrGI TGTACA 1 cut(s) 1396
BsrI ACTGG 3 cut(s) 209, 283, 1232
BssECI CCNNGG 5 cut(s) 249, 585, 1420, 1602, 1614
BssSI CACGAG 1 cut(s) 233
BssT1I CCWWGG 2 cut(s) 585, 1602
Bst2BI CACGAG 1 cut(s) 233
Bst2UI CCWGG 1 cut(s) 149
Bst4CI ACNGT 6 cut(s) 11, 908, 1017, 1401, 1573, 1883
Bst6I CTCTTC 1 cut(s) 1460
BstAFI CTTAAG 1 cut(s) 683
BstAUI TGTACA 1 cut(s) 1396
BstBI TTCGAA 2 cut(s) 519, 1105
BstC8I GCNNGC 7 cut(s) 563, 658, 986, 1139, 1347, 1518, 1979
BstDEI CTNAG 3 cut(s) 180, 1584, 1691
BstDSI CCRYGG 2 cut(s) 585, 1420
BstF5I GGATG 2 cut(s) 1078, 1113
BstFNI CGCG 3 cut(s) 660, 662, 1899
BstH2I RGCGCY 1 cut(s) 1420
BstHHI GCGC 7 cut(s) 7, 662, 752, 1274, 1419, 1672, 1901
BstMAI GTCTC 3 cut(s) 16, 1517, 1651
BstMWI GCNNNNNNNGC 4 cut(s) 945, 990, 999, 1143
BstNI CCWGG 1 cut(s) 149
BstNSI RCATGY 2 cut(s) 988, 1981
BstSCI CCNGG 4 cut(s) 147, 249, 435, 1557
BstSFI CTRYAG 5 cut(s) 76, 604, 777, 921, 1935
BstUI CGCG 3 cut(s) 660, 662, 1899
BstV1I GCAGC 9 cut(s) 256, 788, 907, 940, 1423, 1426, 1684, 1784, 1795
BstV2I GAAGAC 3 cut(s) 357, 549, 1877
BstX2I RGATCY 3 cut(s) 722, 1186, 1951
BstYI RGATCY 3 cut(s) 722, 1186, 1951
BsuI GTATCC 2 cut(s) 1244, 1744
BsuRI GGCC 4 cut(s) 115, 1146, 1697, 1946
BtgI CCRYGG 2 cut(s) 585, 1420
BtsCI GGATG 2 cut(s) 1078, 1113
BtsI GCAGTG 1 cut(s) 1132
BtsIMutI CAGTG 3 cut(s) 845, 1132, 1464
Cac8I GCNNGC 7 cut(s) 563, 658, 986, 1139, 1347, 1518, 1979
CciI TCATGA 2 cut(s) 549, 1405
CfoI GCGC 7 cut(s) 7, 662, 752, 1274, 1419, 1672, 1901
Cfr13I GGNCC 5 cut(s) 397, 1008, 1205, 1695, 1944
CseI GACGC 2 cut(s) 1165, 1664
Csp6I GTAC 7 cut(s) 143, 415, 443, 598, 737, 1397, 1717
CviAII CATG 9 cut(s) 550, 586, 985, 1069, 1082, 1406, 1579, 1641, 1978
CviQI GTAC 7 cut(s) 143, 415, 443, 598, 737, 1397, 1717
DdeI CTNAG 3 cut(s) 180, 1584, 1691
DrdI GACNNNNNNGTC 1 cut(s) 253
DseDI GACNNNNNNGTC 1 cut(s) 253
Eam1104I CTCTTC 1 cut(s) 1460
EarI CTCTTC 1 cut(s) 1460
EciI GGCGGA 2 cut(s) 926, 1535
Ecl136II GAGCTC 1 cut(s) 334
Eco130I CCWWGG 2 cut(s) 585, 1602
Eco24I GRGCYC 1 cut(s) 336
Eco31I GGTCTC 1 cut(s) 16
Eco32I GATATC 1 cut(s) 1182
Eco47I GGWCC 3 cut(s) 397, 1008, 1205
Eco53kI GAGCTC 1 cut(s) 334
Eco57I CTGAAG 2 cut(s) 498, 1296
Eco88I CYCGRG 1 cut(s) 1613
EcoICRI GAGCTC 1 cut(s) 334
EcoO109I RGGNCCY 1 cut(s) 1008
EcoRI GAATTC 1 cut(s) 515
EcoRII CCWGG 1 cut(s) 147
EcoRV GATATC 1 cut(s) 1182
EcoT14I CCWWGG 2 cut(s) 585, 1602
EcoT22I ATGCAT 2 cut(s) 1726, 1983
EcoT38I GRGCYC 1 cut(s) 336
ErhI CCWWGG 2 cut(s) 585, 1602
Esp3I CGTCTC 2 cut(s) 1517, 1651
FaeI CATG 9 cut(s) 553, 589, 988, 1072, 1085, 1409, 1582, 1644, 1981
FalI AAGNNNNNCTT 2 cut(s) 1289, 1321
FaqI GGGAC 2 cut(s) 114, 994
FatI CATG 9 cut(s) 549, 585, 984, 1068, 1081, 1405, 1578, 1640, 1977
FbaI TGATCA 2 cut(s) 835, 1626
FokI GGATG 2 cut(s) 1085, 1100
FriOI GRGCYC 1 cut(s) 336
FspBI CTAG 2 cut(s) 764, 1958
GlaI GCGC 7 cut(s) 6, 661, 751, 1273, 1418, 1671, 1900
GsaI CCCAGC 1 cut(s) 960
HaeII RGCGCY 1 cut(s) 1420
HaeIII GGCC 4 cut(s) 115, 1146, 1697, 1946
HapII CCGG 3 cut(s) 251, 437, 1559
HgaI GACGC 2 cut(s) 1165, 1664
HhaI GCGC 7 cut(s) 7, 662, 752, 1274, 1419, 1672, 1901
Hin1II CATG 9 cut(s) 553, 589, 988, 1072, 1085, 1409, 1582, 1644, 1981
Hin6I GCGC 7 cut(s) 5, 660, 750, 1272, 1417, 1670, 1899
HinP1I GCGC 7 cut(s) 5, 660, 750, 1272, 1417, 1670, 1899
HincII GTYRAC 1 cut(s) 867
HindII GTYRAC 1 cut(s) 867
HindIII AAGCTT 1 cut(s) 1139
HinfI GANTC 8 cut(s) 28, 254, 425, 1063, 1097, 1119, 1363, 1687
HpaII CCGG 3 cut(s) 251, 437, 1559
HphI GGTGA 5 cut(s) 23, 1114, 1436, 1450, 1604
Hpy166II GTNNAC 3 cut(s) 622, 867, 1567
Hpy188I TCNGA 9 cut(s) 478, 904, 1118, 1186, 1290, 1447, 1487, 1686, 1750
Hpy8I GTNNAC 3 cut(s) 622, 867, 1567
Hpy99I CGWCG 3 cut(s) 59, 667, 1159
HpyCH4III ACNGT 6 cut(s) 11, 908, 1017, 1401, 1573, 1883
HpyCH4IV ACGT 2 cut(s) 556, 1563
HpyCH4V TGCA 7 cut(s) 923, 1137, 1259, 1724, 1772, 1834, 1981
HpyF10VI GCNNNNNNNGC 4 cut(s) 945, 990, 999, 1143
HpyF3I CTNAG 3 cut(s) 180, 1584, 1691
HpySE526I ACGT 2 cut(s) 556, 1563
Hsp92II CATG 9 cut(s) 553, 589, 988, 1072, 1085, 1409, 1582, 1644, 1981
HspAI GCGC 7 cut(s) 5, 660, 750, 1272, 1417, 1670, 1899
Ksp22I TGATCA 2 cut(s) 835, 1626
LmnI GCTCC 2 cut(s) 1488, 1865
Lsp1109I GCAGC 9 cut(s) 256, 788, 907, 940, 1423, 1426, 1684, 1784, 1795
MaeI CTAG 2 cut(s) 764, 1958
MaeII ACGT 2 cut(s) 556, 1563
MaeIII GTNAC 3 cut(s) 970, 1084, 1822
MboII GAAGA 9 cut(s) 357, 554, 926, 1181, 1447, 1720, 1877, 1961, 1976
MfeI CAATTG 1 cut(s) 1835
MflI RGATCY 3 cut(s) 722, 1186, 1951
MhlI GDGCHC 2 cut(s) 336, 698
MlyI GAGTC 2 cut(s) 37, 263
MmeI TCCRAC 3 cut(s) 1212, 1470, 1664
Mph1103I ATGCAT 2 cut(s) 1726, 1983
MroXI GAANNNNTTC 2 cut(s) 210, 1101
MseI TTAA 3 cut(s) 684, 932, 1926
MslI CAYNNNNRTG 1 cut(s) 12
MspCI CTTAAG 1 cut(s) 683
MspI CCGG 3 cut(s) 251, 437, 1559
MspR9I CCNGG 4 cut(s) 149, 251, 437, 1559
MunI CAATTG 1 cut(s) 1835
Mva1269I GAATGC 1 cut(s) 1724
MvaI CCWGG 1 cut(s) 149
MvnI CGCG 3 cut(s) 660, 662, 1899
MwoI GCNNNNNNNGC 4 cut(s) 945, 990, 999, 1143
NciI CCSGG 3 cut(s) 251, 437, 1559
NcoI CCATGG 1 cut(s) 585
NlaIII CATG 9 cut(s) 553, 589, 988, 1072, 1085, 1409, 1582, 1644, 1981
NlaIV GGNNCC 4 cut(s) 1010, 1207, 1507, 1945
NmeAIII GCCGAG 1 cut(s) 140
NmuCI GTSAC 1 cut(s) 970
NsiI ATGCAT 2 cut(s) 1726, 1983
NspI RCATGY 2 cut(s) 988, 1981
NspV TTCGAA 2 cut(s) 519, 1105
PaeI GCATGC 2 cut(s) 988, 1981
PagI TCATGA 2 cut(s) 549, 1405
PcsI WCGNNNNNNNCGW 1 cut(s) 1569
PctI GAATGC 1 cut(s) 1724
PdmI GAANNNNTTC 2 cut(s) 210, 1101
PfeI GAWTC 6 cut(s) 425, 1063, 1097, 1119, 1363, 1687
PflFI GACNNNGTC 1 cut(s) 27
PfoI TCCNGGA 2 cut(s) 147, 435
PleI GAGTC 2 cut(s) 36, 262
PpsI GAGTC 2 cut(s) 36, 262
PpuMI RGGWCCY 1 cut(s) 1008
PsiI TTATAA 1 cut(s) 1815
Psp124BI GAGCTC 1 cut(s) 336
Psp1406I AACGTT 1 cut(s) 556
Psp5II RGGWCCY 1 cut(s) 1008
Psp6I CCWGG 1 cut(s) 147
PspFI CCCAGC 1 cut(s) 956
PspGI CCWGG 1 cut(s) 147
PspN4I GGNNCC 4 cut(s) 1010, 1207, 1507, 1945
PspPI GGNCC 5 cut(s) 397, 1008, 1205, 1695, 1944
PspPPI RGGWCCY 1 cut(s) 1008
PsrI GAACNNNNNNTAC 2 cut(s) 398, 430
PstI CTGCAG 1 cut(s) 925
PsuI RGATCY 3 cut(s) 722, 1186, 1951
PsyI GACNNNGTC 1 cut(s) 27
RsaI GTAC 7 cut(s) 144, 416, 444, 599, 738, 1398, 1718
RsaNI GTAC 7 cut(s) 143, 415, 443, 598, 737, 1397, 1717
RseI CAYNNNNRTG 1 cut(s) 12
SacI GAGCTC 1 cut(s) 336
SaqAI TTAA 3 cut(s) 684, 932, 1926
Sau96I GGNCC 5 cut(s) 397, 1008, 1205, 1695, 1944
ScaI AGTACT 1 cut(s) 144
SchI GAGTC 2 cut(s) 37, 263
ScrFI CCNGG 4 cut(s) 149, 251, 437, 1559
SduI GDGCHC 2 cut(s) 336, 698
SfcI CTRYAG 5 cut(s) 76, 604, 777, 921, 1935
SfuI TTCGAA 2 cut(s) 519, 1105
SinI GGWCC 3 cut(s) 397, 1008, 1205
SmiMI CAYNNNNRTG 1 cut(s) 12
SmlI CTYRAG 6 cut(s) 401, 458, 683, 691, 1162, 1907
SmoI CTYRAG 6 cut(s) 401, 458, 683, 691, 1162, 1907
SpeI ACTAGT 1 cut(s) 1957
SphI GCATGC 2 cut(s) 988, 1981
SsiI CCGC 7 cut(s) 266, 381, 747, 937, 940, 1520, 1650
SspMI CTAG 2 cut(s) 764, 1958
SstI GAGCTC 1 cut(s) 336
StyD4I CCNGG 4 cut(s) 147, 249, 435, 1557
StyI CCWWGG 2 cut(s) 585, 1602
TaaI ACNGT 6 cut(s) 11, 908, 1017, 1401, 1573, 1883
TaiI ACGT 2 cut(s) 559, 1566
TaqI TCGA 6 cut(s) 139, 323, 519, 1105, 1655, 1797
TaqII GACCGA 1 cut(s) 40
TatI WGTACW 4 cut(s) 142, 736, 1396, 1716
TauI GCSGC 4 cut(s) 269, 384, 750, 942
TfiI GAWTC 6 cut(s) 425, 1063, 1097, 1119, 1363, 1687
Tru1I TTAA 3 cut(s) 684, 932, 1926
Tru9I TTAA 3 cut(s) 684, 932, 1926
TscAI CASTG 3 cut(s) 845, 1139, 1471
TseFI GTSAC 1 cut(s) 970
TseI GCWGC 9 cut(s) 269, 801, 920, 953, 1411, 1414, 1672, 1772, 1783
Tsp45I GTSAC 1 cut(s) 970
TspDTI ATGAA 9 cut(s) 566, 894, 1070, 1233, 1376, 1394, 1567, 1629, 1778
TspRI CASTG 3 cut(s) 845, 1139, 1471
Tth111I GACNNNGTC 1 cut(s) 27
Vha464I CTTAAG 1 cut(s) 683
VpaK11BI GGWCC 3 cut(s) 397, 1008, 1205
XapI RAATTY 3 cut(s) 41, 515, 1766
XceI RCATGY 2 cut(s) 988, 1981
XcmI CCANNNNNNNNNTGG 1 cut(s) 634
XmnI GAANNNNTTC 2 cut(s) 210, 1101
XspI CTAG 2 cut(s) 764, 1958
ZrmI AGTACT 1 cut(s) 144
Zsp2I ATGCAT 2 cut(s) 1726, 1983
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.