Rroxscaffold_1G00003280

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000466
Physical Location & Seq
Forward (+)
3916118 .. 3916750
633 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00003280.1

Sequence Viewer

Length: 633 bp
ATGAAGCGGGGTCCAAATCCAATACGGAAGGATGATAATGATCCAGCATCTCTTCCTTCATCAGCAGCTGAAGCTTCATCATCATCACCTCCTCCTCCTAAGCAGAAGGATGATCCAGCATCTCTTCCTTCATCATCAGCTGAAGCATCATCATCATCACCACCTCCTCCTCCTCCTGGCCTGGAGTATGATGTGTTTTTGAGTTTCAGGGGTGCTGACACTCGCAAGGGTATCACATCTGATCTATACCATCAACTGCAGAGGAGTGGAATTAAAACATTCATGGATGATCCAGATCTTGAAGTTGGGGATGCTATTTCTCCCACTCTCATTGCAGCAATTGAACAATCACGGTTTGCAATTGTTGTTCTCTCGCGAGACTACGCTTCGTCTACGTGGTGTTTGGAGGAACTTGCAAAGATCTGTGAGTGCCTGAAAGATCAGAACAGAATTCTTCCACTCTTTTATCATGTGGAGCCCTCTGATATTCGATATCAGAAGAAAAGTTTCGAAGAAGCTTTCTCTAAACATGAAACCTCTGGACGATGCAGATCGGAGAAGGTGAAGCAATGGAGAGATGCTTTAAACAAAGTGGCCGATTTTTCTGGCTGGAATACGCAGGATTATAAGTAA

Protein Analysis

210

Amino Acids

23.32

Weight (kDa)

5.48

Isoelectric Point (pI)

65.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 63 - 208 1.8e-44 TIR domain
TIR_2 PF13676 65 - 163 1.2e-17 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000466)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g35831 FvH4_3g44041 FvH4_5g15070 FvH4_5g15070 FvH4_7g08891
malus_domestica MD12G1125500.v1.1
prunus_persica Prupe.2G112700_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0322841 RchiOBHm_Chr1g0323391 RchiOBHm_Chr1g0327991 RchiOBHm_Chr1g0328961 RchiOBHm_Chr5g0080091 RchiOBHm_Chr6g0244841 RchiOBHm_Chr6g0250491
rosa_laevigata RLG00000024005 RLG00000024011 RLG00000024013 RLG00000024016 RLG00000024020 RLG00000024393 RLG00000030320 RLG00000030327 RLG00000030347 RLG00000030348 RLG00000030353 RLG00000030374 RLG00000034792 RLG00000035452 RLG00000036087 RLG00000036760 RLG00000036762 RLG00000036764 RLG00000036766 RLG00000036814 RLG00000036815 RLG00000036818 RLG00000036827 RLG00000036828 RLG00000036830 RLG00000036831 RLG00000036833 RLG00000036835
rosa_multiflora Rmu_co8082032.1_g000001 Rmu_co8162460.1_g000001 Rmu_co8167342.1_g000001 Rmu_co8180788.1_g000001 Rmu_co8300873.1_g000001 Rmu_sc0000252.1_g000008 Rmu_sc0000588.1_g000050 Rmu_sc0000595.1_g000020 Rmu_sc0000749.1_g000016 Rmu_sc0000749.1_g000039 Rmu_sc0000908.1_g000020 Rmu_sc0001148.1_g000040 Rmu_sc0001148.1_g000041 Rmu_sc0001226.1_g000014 Rmu_sc0001639.1_g000003 Rmu_sc0002075.1_g000005 Rmu_sc0002132.1_g000044 Rmu_sc0002634.1_g000026 Rmu_sc0003418.1_g000010 Rmu_sc0007793.1_g000030 Rmu_sc0012920.1_g000001 Rmu_sc0016164.1_g000016 Rmu_sc0019190.1_g000006 Rmu_sc0019291.1_g000001 Rmu_sc0021169.1_g000003 Rmu_sc0040474.1_g000001
rosa_roxburghii Rroxscaffold_1G00003030 Rroxscaffold_1G00003240 Rroxscaffold_1G00003280 Rroxscaffold_1G00003530 Rroxscaffold_1G00003600 Rroxscaffold_1G00003920 Rroxscaffold_3G00235100 Rroxscaffold_3G00240330 Rroxscaffold_4G00326470 Rroxscaffold_4G00326760 Rroxscaffold_4G00327070 Rroxscaffold_6G00393840 Rroxscaffold_6G00408110 Rroxscaffold_6G00408180
rosa_rugosa Rorug01G0036300 Rorug01G0038900 Rorug02G0108200 Rorug05G0459600 Rorug07G0125600 Rorug07G0204700
rosa_samantha Rh1DG060500 Rh6BG033900
rosa_wichuraiana Rw0G008150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 627
AccI GTMKAC 1 cut(s) 392
AccII CGCG 1 cut(s) 376
AciI CCGC 1 cut(s) 7
AclWI GGATC 3 cut(s) 35, 107, 284
AcoI YGGCCR 1 cut(s) 594
AcsI RAATTY 1 cut(s) 450
AcuI CTGAAG 2 cut(s) 90, 162
AfiI CCNNNNNNNGG 1 cut(s) 176
AgsI TTSAA 2 cut(s) 302, 344
AjnI CCWGG 2 cut(s) 175, 180
AluBI AGCT 4 cut(s) 68, 74, 140, 518
AluI AGCT 4 cut(s) 68, 74, 140, 518
Alw26I GTCTC 1 cut(s) 372
AlwI GGATC 3 cut(s) 35, 107, 284
AlwNI CAGNNNCTG 1 cut(s) 68
AoxI GGCC 2 cut(s) 178, 594
ApeKI GCWGC 2 cut(s) 65, 335
ApoI RAATTY 1 cut(s) 450
Asp700I GAANNNNTTC 1 cut(s) 506
AspS9I GGNCC 1 cut(s) 11
AsuHPI GGTGA 3 cut(s) 78, 150, 574
AsuII TTCGAA 1 cut(s) 510
AvaII GGWCC 1 cut(s) 11
BanII GRGCYC 1 cut(s) 480
BbvI GCAGC 2 cut(s) 77, 347
BccI CCATC 1 cut(s) 258
BciT130I CCWGG 2 cut(s) 177, 182
BcoDI GTCTC 1 cut(s) 372
BfmI CTRYAG 1 cut(s) 257
BglII AGATCT 2 cut(s) 295, 420
BisI GCNGC 2 cut(s) 66, 336
BlsI GCNGC 2 cut(s) 67, 337
Bme1390I CCNGG 2 cut(s) 177, 182
Bme18I GGWCC 1 cut(s) 11
BmgT120I GGNCC 1 cut(s) 11
BmiI GGNNCC 2 cut(s) 12, 477
BmrFI CCNGG 2 cut(s) 177, 182
BmsI GCATC 6 cut(s) 56, 128, 155, 301, 536, 568
BpmI CTGGAG 1 cut(s) 203
Bpu10I CCTNAGC 1 cut(s) 99
Bpu14I TTCGAA 1 cut(s) 510
BsaAI YACGTR 1 cut(s) 396
BsaBI GATNNNNATC 3 cut(s) 39, 294, 550
Bsc4I CCNNNNNNNGG 1 cut(s) 176
Bse3DI GCAATG 2 cut(s) 330, 575
Bse8I GATNNNNATC 3 cut(s) 39, 294, 550
BseBI CCWGG 2 cut(s) 177, 182
BseGI GGATG 4 cut(s) 37, 115, 292, 316
BseJI GATNNNNATC 3 cut(s) 39, 294, 550
BseLI CCNNNNNNNGG 1 cut(s) 176
BseMI GCAATG 2 cut(s) 330, 575
BseRI GAGGAG 6 cut(s) 81, 84, 156, 159, 162, 277
BseXI GCAGC 2 cut(s) 77, 347
Bsh1236I CGCG 1 cut(s) 376
BshFI GGCC 2 cut(s) 180, 596
BslI CCNNNNNNNGG 1 cut(s) 176
BsmAI GTCTC 1 cut(s) 372
BsnI GGCC 2 cut(s) 180, 596
Bsp119I TTCGAA 1 cut(s) 510
Bsp1286I GDGCHC 1 cut(s) 480
Bsp143I GATC 8 cut(s) 40, 112, 241, 289, 295, 420, 439, 551
Bsp68I TCGCGA 1 cut(s) 376
BspACI CCGC 1 cut(s) 7
BspANI GGCC 2 cut(s) 180, 596
BspFNI CGCG 1 cut(s) 376
BspLI GGNNCC 2 cut(s) 12, 477
BspMAI CTGCAG 1 cut(s) 261
BspPI GGATC 3 cut(s) 35, 107, 284
BspT104I TTCGAA 1 cut(s) 510
BsrDI GCAATG 2 cut(s) 330, 575
BssMI GATC 8 cut(s) 40, 112, 241, 289, 295, 420, 439, 551
Bst2UI CCWGG 2 cut(s) 177, 182
Bst4CI ACNGT 1 cut(s) 354
Bst6I CTCTTC 2 cut(s) 57, 129
BstBAI YACGTR 1 cut(s) 396
BstBI TTCGAA 1 cut(s) 510
BstDEI CTNAG 1 cut(s) 99
BstF5I GGATG 4 cut(s) 37, 115, 292, 316
BstFNI CGCG 1 cut(s) 376
BstKTI GATC 8 cut(s) 43, 115, 244, 292, 298, 423, 442, 554
BstMAI GTCTC 1 cut(s) 372
BstMBI GATC 8 cut(s) 40, 112, 241, 289, 295, 420, 439, 551
BstMWI GCNNNNNNNGC 1 cut(s) 71
BstNI CCWGG 2 cut(s) 177, 182
BstSCI CCNGG 2 cut(s) 175, 180
BstSFI CTRYAG 1 cut(s) 257
BstUI CGCG 1 cut(s) 376
BstV1I GCAGC 2 cut(s) 77, 347
BstX2I RGATCY 2 cut(s) 295, 420
BstYI RGATCY 2 cut(s) 295, 420
BsuRI GGCC 2 cut(s) 180, 596
BtsCI GGATG 4 cut(s) 37, 115, 292, 316
BtuMI TCGCGA 1 cut(s) 376
CaiI CAGNNNCTG 1 cut(s) 68
Cfr13I GGNCC 1 cut(s) 11
CspCI CAANNNNNGTGG 2 cut(s) 313, 348
CviAII CATG 3 cut(s) 283, 470, 530
CviJI RGCY 8 cut(s) 68, 74, 140, 180, 478, 518, 596, 609
CviKI_1 RGCY 8 cut(s) 68, 74, 140, 180, 478, 518, 596, 609
DdeI CTNAG 1 cut(s) 99
DpnI GATC 8 cut(s) 42, 114, 243, 291, 297, 422, 441, 553
DpnII GATC 8 cut(s) 40, 112, 241, 289, 295, 420, 439, 551
DraI TTTAAA 1 cut(s) 585
EaeI YGGCCR 1 cut(s) 594
Eam1104I CTCTTC 2 cut(s) 57, 129
EarI CTCTTC 2 cut(s) 57, 129
Eco24I GRGCYC 1 cut(s) 480
Eco32I GATATC 1 cut(s) 494
Eco47I GGWCC 1 cut(s) 11
Eco57I CTGAAG 2 cut(s) 90, 162
EcoRI GAATTC 1 cut(s) 450
EcoRII CCWGG 2 cut(s) 175, 180
EcoRV GATATC 1 cut(s) 494
EcoT38I GRGCYC 1 cut(s) 480
FaeI CATG 3 cut(s) 286, 473, 533
FaiI YATR 6 cut(s) 189, 247, 284, 471, 531, 627
FatI CATG 3 cut(s) 282, 469, 529
FblI GTMKAC 1 cut(s) 392
Fnu4HI GCNGC 2 cut(s) 66, 336
FokI GGATG 4 cut(s) 44, 122, 299, 323
FriOI GRGCYC 1 cut(s) 480
Fsp4HI GCNGC 2 cut(s) 66, 336
GluI GCNGC 2 cut(s) 66, 336
GsuI CTGGAG 1 cut(s) 203
HaeIII GGCC 2 cut(s) 180, 596
Hin1II CATG 3 cut(s) 286, 473, 533
HindIII AAGCTT 2 cut(s) 72, 516
HphI GGTGA 3 cut(s) 78, 150, 574
Hpy166II GTNNAC 1 cut(s) 393
Hpy188I TCNGA 5 cut(s) 241, 444, 484, 498, 556
Hpy188III TCNNGA 4 cut(s) 293, 299, 375, 540
Hpy8I GTNNAC 1 cut(s) 393
HpyAV CCTTC 5 cut(s) 22, 66, 100, 138, 553
HpyCH4III ACNGT 1 cut(s) 354
HpyCH4IV ACGT 1 cut(s) 395
HpyCH4V TGCA 5 cut(s) 259, 335, 359, 416, 549
HpyF10VI GCNNNNNNNGC 1 cut(s) 71
HpyF3I CTNAG 1 cut(s) 99
HpySE526I ACGT 1 cut(s) 395
Hsp92II CATG 3 cut(s) 286, 473, 533
Kzo9I GATC 8 cut(s) 40, 112, 241, 289, 295, 420, 439, 551
LmnI GCTCC 1 cut(s) 475
Lsp1109I GCAGC 2 cut(s) 77, 347
LweI GCATC 6 cut(s) 56, 128, 155, 301, 536, 568
MaeII ACGT 1 cut(s) 395
MalI GATC 8 cut(s) 42, 114, 243, 291, 297, 422, 441, 553
MboI GATC 8 cut(s) 40, 112, 241, 289, 295, 420, 439, 551
MboII GAAGA 5 cut(s) 44, 116, 446, 511, 524
MfeI CAATTG 2 cut(s) 339, 360
MflI RGATCY 2 cut(s) 295, 420
MhlI GDGCHC 1 cut(s) 480
MluCI AATT 4 cut(s) 270, 339, 360, 450
MroXI GAANNNNTTC 1 cut(s) 506
MseI TTAA 2 cut(s) 273, 584
MspA1I CMGCKG 2 cut(s) 68, 140
MspR9I CCNGG 2 cut(s) 177, 182
MunI CAATTG 2 cut(s) 339, 360
MvaI CCWGG 2 cut(s) 177, 182
MvnI CGCG 1 cut(s) 376
MwoI GCNNNNNNNGC 1 cut(s) 71
NdeII GATC 8 cut(s) 40, 112, 241, 289, 295, 420, 439, 551
NlaIII CATG 3 cut(s) 286, 473, 533
NlaIV GGNNCC 2 cut(s) 12, 477
NruI TCGCGA 1 cut(s) 376
NspV TTCGAA 1 cut(s) 510
PdmI GAANNNNTTC 1 cut(s) 506
PkrI GCNGC 2 cut(s) 67, 337
Ppu21I YACGTR 1 cut(s) 396
PsiI TTATAA 1 cut(s) 627
Psp6I CCWGG 2 cut(s) 175, 180
PspGI CCWGG 2 cut(s) 175, 180
PspN4I GGNNCC 2 cut(s) 12, 477
PspPI GGNCC 1 cut(s) 11
PstI CTGCAG 1 cut(s) 261
PstNI CAGNNNCTG 1 cut(s) 68
PsuI RGATCY 2 cut(s) 295, 420
PvuII CAGCTG 2 cut(s) 68, 140
RruI TCGCGA 1 cut(s) 376
SaqAI TTAA 2 cut(s) 273, 584
SatI GCNGC 2 cut(s) 66, 336
Sau3AI GATC 8 cut(s) 40, 112, 241, 289, 295, 420, 439, 551
Sau96I GGNCC 1 cut(s) 11
ScrFI CCNGG 2 cut(s) 177, 182
SduI GDGCHC 1 cut(s) 480
SetI ASST 9 cut(s) 70, 76, 91, 142, 166, 398, 520, 539, 564
SfaNI GCATC 6 cut(s) 56, 128, 155, 301, 536, 568
SfcI CTRYAG 1 cut(s) 257
SfuI TTCGAA 1 cut(s) 510
SinI GGWCC 1 cut(s) 11
Sse9I AATT 4 cut(s) 270, 339, 360, 450
SsiI CCGC 1 cut(s) 7
StyD4I CCNGG 2 cut(s) 175, 180
TaaI ACNGT 1 cut(s) 354
TaiI ACGT 1 cut(s) 398
TaqI TCGA 2 cut(s) 490, 510
TasI AATT 4 cut(s) 270, 339, 360, 450
Tru1I TTAA 2 cut(s) 273, 584
Tru9I TTAA 2 cut(s) 273, 584
TseI GCWGC 2 cut(s) 65, 335
TspDTI ATGAA 6 cut(s) 17, 48, 66, 120, 271, 546
TspGWI ACGGA 1 cut(s) 40
VpaK11BI GGWCC 1 cut(s) 11
XapI RAATTY 1 cut(s) 450
XmiI GTMKAC 1 cut(s) 392
XmnI GAANNNNTTC 1 cut(s) 506
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.