RLG00000036830

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000466
Physical Location & Seq
Reverse (-)
85364802 .. 85367551
2750 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000036830

Sequence Viewer

Length: 615 bp
ATGTGGGATCTCAGTGACTCTCCTGATCAGGGAAACAATGGCACCACCTCGGCCGCCGTGCCGGTTGCTTCTTCCTCTTCTCTATCATCAGCTGTTGAGTGGAAGTATGATGTACTTTTGAGTTTCAGGGGTCCTGACACTCGCAAGGGTATCACATTTGAACTATACGATCGACTGCAGAGGAGGGGAATTAAAACATTCATGGATGATCGAAATCTTGAAGTAGGGGATGTTATTTCTCCAATTCTCTTAGCAGCAATTAGAGAATCAAGGTTTGCAATTGTTGTTCTCTCACAAAATTATGCTTCTTCTACTTGGTGTTTGGAGGAACGTAGAGAGATTTGTCTATACATGAAAGACCACAGTAGAATCCTGCCCCTTTTTTATCAGGTTGATCCTACTGATGTGCGATATCAGAAGAGGAGTTTTGAAGAAGCTTTCACTAGGCATGAAACCTCTGGACAACATGAATCAGAGAAGGTGGAAAAGTGGAAAGCTGCTTTAAACAAAGTGGCCAATATCTCTGGGTGGAATACAAATGATCATAATCGATCAAGAGGTGCTTTGATGAAAGTTATTGTGAATTGGAATCCATGTACATTGCACGTTCGCTAA

Protein Analysis

205

Amino Acids

23.4

Weight (kDa)

7.74

Isoelectric Point (pI)

53.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 35 - 183 2.5e-43 TIR domain
TIR_2 PF13676 38 - 133 1.6e-14 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000466)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g35831 FvH4_3g44041 FvH4_5g15070 FvH4_5g15070 FvH4_7g08891
malus_domestica MD12G1125500.v1.1
prunus_persica Prupe.2G112700_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0322841 RchiOBHm_Chr1g0323391 RchiOBHm_Chr1g0327991 RchiOBHm_Chr1g0328961 RchiOBHm_Chr5g0080091 RchiOBHm_Chr6g0244841 RchiOBHm_Chr6g0250491
rosa_laevigata RLG00000024005 RLG00000024011 RLG00000024013 RLG00000024016 RLG00000024020 RLG00000024393 RLG00000030320 RLG00000030327 RLG00000030347 RLG00000030348 RLG00000030353 RLG00000030374 RLG00000034792 RLG00000035452 RLG00000036087 RLG00000036760 RLG00000036762 RLG00000036764 RLG00000036766 RLG00000036814 RLG00000036815 RLG00000036818 RLG00000036827 RLG00000036828 RLG00000036830 RLG00000036831 RLG00000036833 RLG00000036835
rosa_multiflora Rmu_co8082032.1_g000001 Rmu_co8162460.1_g000001 Rmu_co8167342.1_g000001 Rmu_co8180788.1_g000001 Rmu_co8300873.1_g000001 Rmu_sc0000252.1_g000008 Rmu_sc0000588.1_g000050 Rmu_sc0000595.1_g000020 Rmu_sc0000749.1_g000016 Rmu_sc0000749.1_g000039 Rmu_sc0000908.1_g000020 Rmu_sc0001148.1_g000040 Rmu_sc0001148.1_g000041 Rmu_sc0001226.1_g000014 Rmu_sc0001639.1_g000003 Rmu_sc0002075.1_g000005 Rmu_sc0002132.1_g000044 Rmu_sc0002634.1_g000026 Rmu_sc0003418.1_g000010 Rmu_sc0007793.1_g000030 Rmu_sc0012920.1_g000001 Rmu_sc0016164.1_g000016 Rmu_sc0019190.1_g000006 Rmu_sc0019291.1_g000001 Rmu_sc0021169.1_g000003 Rmu_sc0040474.1_g000001
rosa_roxburghii Rroxscaffold_1G00003030 Rroxscaffold_1G00003240 Rroxscaffold_1G00003280 Rroxscaffold_1G00003530 Rroxscaffold_1G00003600 Rroxscaffold_1G00003920 Rroxscaffold_3G00235100 Rroxscaffold_3G00240330 Rroxscaffold_4G00326470 Rroxscaffold_4G00326760 Rroxscaffold_4G00327070 Rroxscaffold_6G00393840 Rroxscaffold_6G00408110 Rroxscaffold_6G00408180
rosa_rugosa Rorug01G0036300 Rorug01G0038900 Rorug02G0108200 Rorug05G0459600 Rorug07G0125600 Rorug07G0204700
rosa_samantha Rh1DG060500 Rh6BG033900
rosa_wichuraiana Rw0G008150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 41
AciI CCGC 1 cut(s) 54
AclWI GGATC 2 cut(s) 15, 389
AcoI YGGCCR 2 cut(s) 51, 513
AfaI GTAC 2 cut(s) 114, 598
AfiI CCNNNNNNNGG 1 cut(s) 29
AgsI TTSAA 3 cut(s) 161, 221, 431
AluBI AGCT 3 cut(s) 92, 437, 497
AluI AGCT 3 cut(s) 92, 437, 497
AlwI GGATC 2 cut(s) 15, 389
AoxI GGCC 2 cut(s) 51, 513
ApeKI GCWGC 2 cut(s) 254, 497
AspS9I GGNCC 1 cut(s) 131
AvaII GGWCC 1 cut(s) 131
BalI TGGCCA 1 cut(s) 515
BanI GGYRCC 1 cut(s) 41
BbvI GCAGC 2 cut(s) 266, 484
BceAI ACGGC 1 cut(s) 41
BclI TGATCA 2 cut(s) 25, 541
BfaI CTAG 1 cut(s) 444
BfmI CTRYAG 1 cut(s) 176
BisI GCNGC 3 cut(s) 54, 255, 498
BlsI GCNGC 3 cut(s) 55, 256, 499
Bme18I GGWCC 1 cut(s) 131
BmgT120I GGNCC 1 cut(s) 131
BmiI GGNNCC 2 cut(s) 43, 132
Bsa29I ATCGAT 1 cut(s) 550
BsaBI GATNNNNATC 2 cut(s) 213, 546
BsaJI CCNNGG 1 cut(s) 48
Bsc4I CCNNNNNNNGG 1 cut(s) 29
Bse118I RCCGGY 1 cut(s) 61
Bse3DI GCAATG 1 cut(s) 599
Bse8I GATNNNNATC 2 cut(s) 213, 546
BseCI ATCGAT 1 cut(s) 550
BseDI CCNNGG 1 cut(s) 48
BseGI GGATG 2 cut(s) 211, 235
BseJI GATNNNNATC 2 cut(s) 213, 546
BseLI CCNNNNNNNGG 1 cut(s) 29
BseMI GCAATG 1 cut(s) 599
BseMII CTCAG 1 cut(s) 25
BseRI GAGGAG 2 cut(s) 196, 436
BseX3I CGGCCG 1 cut(s) 51
BseXI GCAGC 2 cut(s) 266, 484
Bsh1285I CGRYCG 2 cut(s) 54, 172
BshFI GGCC 2 cut(s) 53, 515
BshNI GGYRCC 1 cut(s) 41
BshVI ATCGAT 1 cut(s) 550
BsiEI CGRYCG 2 cut(s) 54, 172
BsiSI CCGG 1 cut(s) 62
BslI CCNNNNNNNGG 1 cut(s) 29
BsnI GGCC 2 cut(s) 53, 515
Bsp1407I TGTACA 1 cut(s) 596
Bsp143I GATC 7 cut(s) 7, 25, 169, 208, 394, 541, 551
BspACI CCGC 1 cut(s) 54
BspANI GGCC 2 cut(s) 53, 515
BspCNI CTCAG 1 cut(s) 24
BspDI ATCGAT 1 cut(s) 550
BspLI GGNNCC 2 cut(s) 43, 132
BspMAI CTGCAG 1 cut(s) 180
BspPI GGATC 2 cut(s) 15, 389
BspT107I GGYRCC 1 cut(s) 41
BsrDI GCAATG 1 cut(s) 599
BsrFI RCCGGY 1 cut(s) 61
BsrGI TGTACA 1 cut(s) 596
BssAI RCCGGY 1 cut(s) 61
BssECI CCNNGG 1 cut(s) 48
BssMI GATC 7 cut(s) 7, 25, 169, 208, 394, 541, 551
Bst4CI ACNGT 1 cut(s) 365
Bst6I CTCTTC 2 cut(s) 82, 413
BstAUI TGTACA 1 cut(s) 596
BstDEI CTNAG 2 cut(s) 11, 250
BstF5I GGATG 2 cut(s) 211, 235
BstKTI GATC 7 cut(s) 10, 28, 172, 211, 397, 544, 554
BstMBI GATC 7 cut(s) 7, 25, 169, 208, 394, 541, 551
BstMCI CGRYCG 2 cut(s) 54, 172
BstSFI CTRYAG 1 cut(s) 176
BstV1I GCAGC 2 cut(s) 266, 484
BstX2I RGATCY 1 cut(s) 7
BstYI RGATCY 1 cut(s) 7
BstZI CGGCCG 1 cut(s) 51
Bsu15I ATCGAT 1 cut(s) 550
BsuRI GGCC 2 cut(s) 53, 515
BsuTUI ATCGAT 1 cut(s) 550
BtsCI GGATG 2 cut(s) 211, 235
BtsIMutI CAGTG 1 cut(s) 19
Cfr10I RCCGGY 1 cut(s) 61
Cfr13I GGNCC 1 cut(s) 131
ClaI ATCGAT 1 cut(s) 550
Csp6I GTAC 2 cut(s) 113, 597
CviAII CATG 5 cut(s) 202, 352, 449, 467, 594
CviJI RGCY 5 cut(s) 53, 92, 437, 497, 515
CviKI_1 RGCY 5 cut(s) 53, 92, 437, 497, 515
CviQI GTAC 2 cut(s) 113, 597
DdeI CTNAG 2 cut(s) 11, 250
DpnI GATC 7 cut(s) 9, 27, 171, 210, 396, 543, 553
DpnII GATC 7 cut(s) 7, 25, 169, 208, 394, 541, 551
DraI TTTAAA 1 cut(s) 504
EaeI YGGCCR 2 cut(s) 51, 513
EagI CGGCCG 1 cut(s) 51
Eam1104I CTCTTC 2 cut(s) 82, 413
EarI CTCTTC 2 cut(s) 82, 413
EclXI CGGCCG 1 cut(s) 51
Eco32I GATATC 1 cut(s) 413
Eco47I GGWCC 1 cut(s) 131
Eco52I CGGCCG 1 cut(s) 51
EcoO109I RGGNCCY 1 cut(s) 131
EcoRV GATATC 1 cut(s) 413
FaeI CATG 5 cut(s) 205, 355, 452, 470, 597
FalI AAGNNNNNCTT 2 cut(s) 547, 579
FatI CATG 5 cut(s) 201, 351, 448, 466, 593
FbaI TGATCA 2 cut(s) 25, 541
Fnu4HI GCNGC 3 cut(s) 54, 255, 498
FokI GGATG 2 cut(s) 218, 242
Fsp4HI GCNGC 3 cut(s) 54, 255, 498
FspBI CTAG 1 cut(s) 444
GluI GCNGC 3 cut(s) 54, 255, 498
HaeIII GGCC 2 cut(s) 53, 515
HapII CCGG 1 cut(s) 62
Hin1II CATG 5 cut(s) 205, 355, 452, 470, 597
HindIII AAGCTT 1 cut(s) 435
HinfI GANTC 5 cut(s) 17, 266, 369, 470, 589
HpaII CCGG 1 cut(s) 62
Hpy188I TCNGA 2 cut(s) 417, 475
Hpy188III TCNNGA 5 cut(s) 23, 134, 218, 459, 555
HpyAV CCTTC 1 cut(s) 472
HpyCH4III ACNGT 1 cut(s) 365
HpyCH4IV ACGT 2 cut(s) 331, 606
HpyCH4V TGCA 3 cut(s) 178, 278, 604
HpyF3I CTNAG 2 cut(s) 11, 250
HpySE526I ACGT 2 cut(s) 331, 606
Hsp92II CATG 5 cut(s) 205, 355, 452, 470, 597
Ksp22I TGATCA 2 cut(s) 25, 541
Kzo9I GATC 7 cut(s) 7, 25, 169, 208, 394, 541, 551
LpnPI CCDG 9 cut(s) 14, 36, 75, 112, 147, 374, 386, 444, 510
Lsp1109I GCAGC 2 cut(s) 266, 484
MaeI CTAG 1 cut(s) 444
MaeII ACGT 2 cut(s) 331, 606
MaeIII GTNAC 1 cut(s) 14
MalI GATC 7 cut(s) 9, 27, 171, 210, 396, 543, 553
MboI GATC 7 cut(s) 7, 25, 169, 208, 394, 541, 551
MboII GAAGA 5 cut(s) 63, 69, 300, 430, 443
MfeI CAATTG 1 cut(s) 279
MflI RGATCY 1 cut(s) 7
MlsI TGGCCA 1 cut(s) 515
MluCI AATT 6 cut(s) 189, 243, 258, 279, 298, 583
MluNI TGGCCA 1 cut(s) 515
MlyI GAGTC 1 cut(s) 11
MnlI CCTC 8 cut(s) 58, 85, 174, 177, 319, 414, 466, 551
Mox20I TGGCCA 1 cut(s) 515
MscI TGGCCA 1 cut(s) 515
MseI TTAA 2 cut(s) 192, 503
Msp20I TGGCCA 1 cut(s) 515
MspA1I CMGCKG 1 cut(s) 92
MspI CCGG 1 cut(s) 62
MunI CAATTG 1 cut(s) 279
NdeII GATC 7 cut(s) 7, 25, 169, 208, 394, 541, 551
NlaIII CATG 5 cut(s) 205, 355, 452, 470, 597
NlaIV GGNNCC 2 cut(s) 43, 132
NmeAIII GCCGAG 1 cut(s) 29
NmuCI GTSAC 1 cut(s) 14
PfeI GAWTC 4 cut(s) 266, 369, 470, 589
PkrI GCNGC 3 cut(s) 55, 256, 499
Ple19I CGATCG 1 cut(s) 172
PleI GAGTC 1 cut(s) 11
PpsI GAGTC 1 cut(s) 11
PpuMI RGGWCCY 1 cut(s) 131
Psp5II RGGWCCY 1 cut(s) 131
PspN4I GGNNCC 2 cut(s) 43, 132
PspPI GGNCC 1 cut(s) 131
PspPPI RGGWCCY 1 cut(s) 131
PstI CTGCAG 1 cut(s) 180
PsuI RGATCY 1 cut(s) 7
PvuI CGATCG 1 cut(s) 172
PvuII CAGCTG 1 cut(s) 92
RsaI GTAC 2 cut(s) 114, 598
RsaNI GTAC 2 cut(s) 113, 597
SaqAI TTAA 2 cut(s) 192, 503
SatI GCNGC 3 cut(s) 54, 255, 498
Sau3AI GATC 7 cut(s) 7, 25, 169, 208, 394, 541, 551
Sau96I GGNCC 1 cut(s) 131
SchI GAGTC 1 cut(s) 11
SfcI CTRYAG 1 cut(s) 176
SinI GGWCC 1 cut(s) 131
Sse9I AATT 6 cut(s) 189, 243, 258, 279, 298, 583
SsiI CCGC 1 cut(s) 54
SspMI CTAG 1 cut(s) 444
TaaI ACNGT 1 cut(s) 365
TaiI ACGT 2 cut(s) 334, 609
TaqI TCGA 3 cut(s) 172, 211, 550
TasI AATT 6 cut(s) 189, 243, 258, 279, 298, 583
TatI WGTACW 2 cut(s) 112, 596
TauI GCSGC 1 cut(s) 56
TfiI GAWTC 4 cut(s) 266, 369, 470, 589
Tru1I TTAA 2 cut(s) 192, 503
Tru9I TTAA 2 cut(s) 192, 503
TscAI CASTG 1 cut(s) 19
TseFI GTSAC 1 cut(s) 14
TseI GCWGC 2 cut(s) 254, 497
Tsp45I GTSAC 1 cut(s) 14
TspDTI ATGAA 5 cut(s) 190, 368, 465, 483, 584
TspRI CASTG 1 cut(s) 19
VpaK11BI GGWCC 1 cut(s) 131
XspI CTAG 1 cut(s) 444
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.